MD10G1066400.v1.1

Protein SHI RELATED SEQUENCE

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0009392
Physical Location & Seq
Forward (+)
9092326 .. 9093733
1408 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1066400.v1.1.491

Sequence Viewer

Length: 690 bp
ATGGTGAGGCAAGGAAGAGGAGGAAGAGAAGAAGAAATAATGGGTTCCAGATGCCAAGACTGTGGGAATCAATCAAAGAAGGAGTGTGTGTACATGAGATGCAGGACTTGCTGTAAGAGCAAAGGGTTTCACTGCCAAACTCACTTCAAAAGTACTTGGGTTCCTGTCCACAGAAGGCGCCACGCTCATCACCAACAGCTTCCTTCTATTGATCCACAGCACCCACCTCACCTTCATCAAGGGCACAACTACAACCCTAGGAGGACAGCAGCTGGACACGTCATCACCAATTCCTCTTCATCTGGGCAAAATCAACAGGTTAACAATTTTCCAGCAGAAGTGAATTCCATGGCCAATTTTCGCTGTGTTCGAGTGCGCTCCGTGGAGGATACGGTCAATCAGCAGGCTTATCAGACAAGTATGGTAATCGGGGGGCATGTATTCAAAGGTATACTTTATGATCAAGGCCCTGATTACACCACTGCAGCTGGCCAAAGTTCCAGCTCCCAGCATGTAATGAATAAGCAAACTAGTAATTTTATTAATGCTACTGCTTCTGCTTCAAATATTACTGCTTCCACTCCATCAGCTGCTGCAGATCATCAAGTGCCACATCATCATCAACTTCCTTCTTCTTATCCGTTGATTCCTTTTAATGCATTCATGCCTGGTACGCAATTTTTCCTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF702

Protein Analysis

230

Amino Acids

25.52

Weight (kDa)

9.36

Isoelectric Point (pI)

51.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF702 PF05142 15 - 158 7.9e-53 Domain of unknown function (DUF702)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 177
AccI GTMKAC 1 cut(s) 451
AclWI GGATC 1 cut(s) 206
AcoI YGGCCR 2 cut(s) 351, 490
AcsI RAATTY 1 cut(s) 343
AcyI GRCGYC 1 cut(s) 178
AfaI GTAC 3 cut(s) 92, 154, 673
AflIII ACRYGT 1 cut(s) 277
AgsI TTSAA 3 cut(s) 148, 445, 564
AhlI ACTAGT 1 cut(s) 530
AjiI CACGTC 1 cut(s) 280
AjnI CCWGG 1 cut(s) 667
AluBI AGCT 5 cut(s) 199, 272, 488, 504, 590
AluI AGCT 5 cut(s) 199, 272, 488, 504, 590
AlwI GGATC 1 cut(s) 206
AlwNI CAGNNNCTG 2 cut(s) 272, 593
AoxI GGCC 3 cut(s) 351, 466, 490
ApeKI GCWGC 4 cut(s) 269, 485, 590, 593
ApoI RAATTY 1 cut(s) 343
AseI ATTAAT 1 cut(s) 543
AspA2I CCTAGG 1 cut(s) 257
AspLEI GCGC 2 cut(s) 180, 378
AspS9I GGNCC 1 cut(s) 467
AsuHPI GGTGA 4 cut(s) 16, 182, 221, 277
AvrII CCTAGG 1 cut(s) 257
BaeGI GKGCMC 1 cut(s) 246
BalI TGGCCA 2 cut(s) 353, 492
BanI GGYRCC 1 cut(s) 177
BbvI GCAGC 4 cut(s) 281, 497, 577, 580
BccI CCATC 1 cut(s) 592
BciT130I CCWGG 1 cut(s) 669
BciVI GTATCC 1 cut(s) 382
BclI TGATCA 1 cut(s) 460
BcuI ACTAGT 1 cut(s) 530
BfaI CTAG 2 cut(s) 258, 531
BfmI CTRYAG 2 cut(s) 483, 594
BfoI RGCGCY 1 cut(s) 181
BfuI GTATCC 1 cut(s) 382
BisI GCNGC 4 cut(s) 270, 486, 591, 594
BlnI CCTAGG 1 cut(s) 257
BlsI GCNGC 4 cut(s) 271, 487, 592, 595
BmcAI AGTACT 1 cut(s) 154
Bme1390I CCNGG 1 cut(s) 669
BmgBI CACGTC 1 cut(s) 280
BmgT120I GGNCC 1 cut(s) 467
BmiI GGNNCC 3 cut(s) 46, 162, 179
BmrFI CCNGG 1 cut(s) 669
BmsI GCATC 2 cut(s) 41, 89
BsaHI GRCGYC 1 cut(s) 178
BsaJI CCNNGG 3 cut(s) 257, 348, 381
BsaXI ACNNNNNCTCC 2 cut(s) 74, 104
BseBI CCWGG 1 cut(s) 669
BseDI CCNNGG 3 cut(s) 257, 348, 381
BseRI GAGGAG 1 cut(s) 33
BseSI GKGCMC 1 cut(s) 246
BseXI GCAGC 4 cut(s) 281, 497, 577, 580
BseYI CCCAGC 1 cut(s) 507
BshFI GGCC 3 cut(s) 353, 468, 492
BshNI GGYRCC 1 cut(s) 177
BsmI GAATGC 1 cut(s) 659
BsnI GGCC 3 cut(s) 353, 468, 492
Bsp1286I GDGCHC 1 cut(s) 246
Bsp1407I TGTACA 1 cut(s) 90
Bsp143I GATC 3 cut(s) 211, 460, 598
Bsp19I CCATGG 1 cut(s) 348
BspANI GGCC 3 cut(s) 353, 468, 492
BspLI GGNNCC 3 cut(s) 46, 162, 179
BspMAI CTGCAG 2 cut(s) 487, 598
BspPI GGATC 1 cut(s) 206
BspT107I GGYRCC 1 cut(s) 177
BsrGI TGTACA 1 cut(s) 90
BssECI CCNNGG 3 cut(s) 257, 348, 381
BssMI GATC 3 cut(s) 211, 460, 598
BssNAI GTATAC 1 cut(s) 452
BssNI GRCGYC 1 cut(s) 178
BssT1I CCWWGG 2 cut(s) 257, 348
Bst1107I GTATAC 1 cut(s) 452
Bst2UI CCWGG 1 cut(s) 669
Bst4CI ACNGT 2 cut(s) 62, 394
Bst6I CTCTTC 3 cut(s) 10, 19, 301
BstACI GRCGYC 1 cut(s) 178
BstAPI GCANNNNNTGC 1 cut(s) 108
BstAUI TGTACA 1 cut(s) 90
BstC8I GCNNGC 2 cut(s) 405, 490
BstDSI CCRYGG 2 cut(s) 348, 381
BstH2I RGCGCY 1 cut(s) 181
BstHHI GCGC 2 cut(s) 180, 378
BstKTI GATC 3 cut(s) 214, 463, 601
BstMBI GATC 3 cut(s) 211, 460, 598
BstMWI GCNNNNNNNGC 3 cut(s) 108, 117, 673
BstNI CCWGG 1 cut(s) 669
BstNSI RCATGY 2 cut(s) 440, 515
BstSCI CCNGG 1 cut(s) 667
BstSFI CTRYAG 2 cut(s) 483, 594
BstSLI GKGCMC 1 cut(s) 246
BstV1I GCAGC 4 cut(s) 281, 497, 577, 580
BstXI CCANNNNNNTGG 1 cut(s) 62
BstZ17I GTATAC 1 cut(s) 452
BsuI GTATCC 1 cut(s) 382
BsuRI GGCC 3 cut(s) 353, 468, 492
BtgI CCRYGG 2 cut(s) 348, 381
BtrI CACGTC 1 cut(s) 280
BtsI GCAGTG 2 cut(s) 130, 480
BtsIMutI CAGTG 2 cut(s) 130, 480
Cac8I GCNNGC 2 cut(s) 405, 490
CaiI CAGNNNCTG 2 cut(s) 272, 593
CfoI GCGC 2 cut(s) 180, 378
Cfr13I GGNCC 1 cut(s) 467
Csp6I GTAC 3 cut(s) 91, 153, 672
CviAII CATG 5 cut(s) 94, 349, 437, 512, 664
CviJI RGCY 9 cut(s) 199, 272, 353, 407, 468, 488, 492, 504, 590
CviKI_1 RGCY 9 cut(s) 199, 272, 353, 407, 468, 488, 492, 504, 590
CviQI GTAC 3 cut(s) 91, 153, 672
DinI GGCGCC 1 cut(s) 179
DpnI GATC 3 cut(s) 213, 462, 600
DpnII GATC 3 cut(s) 211, 460, 598
EaeI YGGCCR 2 cut(s) 351, 490
Eam1104I CTCTTC 3 cut(s) 10, 19, 301
EarI CTCTTC 3 cut(s) 10, 19, 301
Eco130I CCWWGG 2 cut(s) 257, 348
EcoO109I RGGNCCY 1 cut(s) 467
EcoRI GAATTC 1 cut(s) 343
EcoRII CCWGG 1 cut(s) 667
EcoT14I CCWWGG 2 cut(s) 257, 348
EcoT22I ATGCAT 1 cut(s) 661
EgeI GGCGCC 1 cut(s) 179
EheI GGCGCC 1 cut(s) 179
ErhI CCWWGG 2 cut(s) 257, 348
FaeI CATG 5 cut(s) 97, 352, 440, 515, 667
FaiI YATR 8 cut(s) 95, 350, 422, 438, 452, 459, 513, 665
FalI AAGNNNNNCTT 2 cut(s) 438, 470
FatI CATG 5 cut(s) 93, 348, 436, 511, 663
FbaI TGATCA 1 cut(s) 460
FblI GTMKAC 1 cut(s) 451
Fnu4HI GCNGC 4 cut(s) 270, 486, 591, 594
Fsp4HI GCNGC 4 cut(s) 270, 486, 591, 594
FspBI CTAG 2 cut(s) 258, 531
GlaI GCGC 2 cut(s) 179, 377
GluI GCNGC 4 cut(s) 270, 486, 591, 594
GsaI CCCAGC 1 cut(s) 511
HaeII RGCGCY 1 cut(s) 181
HaeIII GGCC 3 cut(s) 353, 468, 492
HhaI GCGC 2 cut(s) 180, 378
Hin1I GRCGYC 1 cut(s) 178
Hin1II CATG 5 cut(s) 97, 352, 440, 515, 667
Hin6I GCGC 2 cut(s) 178, 376
HinP1I GCGC 2 cut(s) 178, 376
HincII GTYRAC 1 cut(s) 322
HindII GTYRAC 1 cut(s) 322
HinfI GANTC 2 cut(s) 67, 646
HpaI GTTAAC 1 cut(s) 322
HphI GGTGA 4 cut(s) 16, 182, 221, 277
Hpy166II GTNNAC 4 cut(s) 91, 169, 322, 452
Hpy188I TCNGA 1 cut(s) 414
Hpy188III TCNNGA 1 cut(s) 48
Hpy8I GTNNAC 4 cut(s) 91, 169, 322, 452
HpyAV CCTTC 5 cut(s) 73, 168, 213, 242, 639
HpyCH4III ACNGT 2 cut(s) 62, 394
HpyCH4IV ACGT 1 cut(s) 279
HpyCH4V TGCA 4 cut(s) 102, 485, 596, 659
HpyF10VI GCNNNNNNNGC 3 cut(s) 108, 117, 673
HpySE526I ACGT 1 cut(s) 279
Hsp92I GRCGYC 1 cut(s) 178
Hsp92II CATG 5 cut(s) 97, 352, 440, 515, 667
HspAI GCGC 2 cut(s) 178, 376
KasI GGCGCC 1 cut(s) 177
Ksp22I TGATCA 1 cut(s) 460
KspAI GTTAAC 1 cut(s) 322
Kzo9I GATC 3 cut(s) 211, 460, 598
LmnI GCTCC 2 cut(s) 383, 509
Lsp1109I GCAGC 4 cut(s) 281, 497, 577, 580
LweI GCATC 2 cut(s) 41, 89
MaeI CTAG 2 cut(s) 258, 531
MaeII ACGT 1 cut(s) 279
MalI GATC 3 cut(s) 213, 462, 600
MboI GATC 3 cut(s) 211, 460, 598
MboII GAAGA 6 cut(s) 27, 36, 41, 44, 288, 624
MhlI GDGCHC 1 cut(s) 246
MlsI TGGCCA 2 cut(s) 353, 492
MluCI AATT 6 cut(s) 289, 325, 343, 355, 535, 677
MluNI TGGCCA 2 cut(s) 353, 492
Mly113I GGCGCC 1 cut(s) 178
MnlI CCTC 6 cut(s) 11, 14, 237, 255, 304, 379
Mox20I TGGCCA 2 cut(s) 353, 492
Mph1103I ATGCAT 1 cut(s) 661
MscI TGGCCA 2 cut(s) 353, 492
MseI TTAA 4 cut(s) 321, 543, 654, 688
Msp20I TGGCCA 2 cut(s) 353, 492
MspA1I CMGCKG 3 cut(s) 272, 488, 590
MspR9I CCNGG 1 cut(s) 669
Mva1269I GAATGC 1 cut(s) 659
MvaI CCWGG 1 cut(s) 669
MwoI GCNNNNNNNGC 3 cut(s) 108, 117, 673
NarI GGCGCC 1 cut(s) 178
NcoI CCATGG 1 cut(s) 348
NdeII GATC 3 cut(s) 211, 460, 598
NlaIII CATG 5 cut(s) 97, 352, 440, 515, 667
NlaIV GGNNCC 3 cut(s) 46, 162, 179
NsiI ATGCAT 1 cut(s) 661
NspI RCATGY 2 cut(s) 440, 515
PcsI WCGNNNNNNNCGW 1 cut(s) 367
PctI GAATGC 1 cut(s) 659
PfeI GAWTC 2 cut(s) 67, 646
PkrI GCNGC 4 cut(s) 271, 487, 592, 595
PluTI GGCGCC 1 cut(s) 181
PshBI ATTAAT 1 cut(s) 543
Psp6I CCWGG 1 cut(s) 667
PspFI CCCAGC 1 cut(s) 507
PspGI CCWGG 1 cut(s) 667
PspN4I GGNNCC 3 cut(s) 46, 162, 179
PspPI GGNCC 1 cut(s) 467
PstI CTGCAG 2 cut(s) 487, 598
PstNI CAGNNNCTG 2 cut(s) 272, 593
PvuII CAGCTG 3 cut(s) 272, 488, 590
RsaI GTAC 3 cut(s) 92, 154, 673
RsaNI GTAC 3 cut(s) 91, 153, 672
SaqAI TTAA 4 cut(s) 321, 543, 654, 688
SatI GCNGC 4 cut(s) 270, 486, 591, 594
Sau3AI GATC 3 cut(s) 211, 460, 598
Sau96I GGNCC 1 cut(s) 467
ScaI AGTACT 1 cut(s) 154
ScrFI CCNGG 1 cut(s) 669
SduI GDGCHC 1 cut(s) 246
SfaNI GCATC 2 cut(s) 41, 89
SfcI CTRYAG 2 cut(s) 483, 594
SfoI GGCGCC 1 cut(s) 179
SpeI ACTAGT 1 cut(s) 530
Sse9I AATT 6 cut(s) 289, 325, 343, 355, 535, 677
SspDI GGCGCC 1 cut(s) 177
SspI AATATT 1 cut(s) 568
SspMI CTAG 2 cut(s) 258, 531
StyD4I CCNGG 1 cut(s) 667
StyI CCWWGG 2 cut(s) 257, 348
TaaI ACNGT 2 cut(s) 62, 394
TaiI ACGT 1 cut(s) 282
TaqI TCGA 1 cut(s) 370
TasI AATT 6 cut(s) 289, 325, 343, 355, 535, 677
TatI WGTACW 2 cut(s) 90, 152
TfiI GAWTC 2 cut(s) 67, 646
Tru1I TTAA 4 cut(s) 321, 543, 654, 688
Tru9I TTAA 4 cut(s) 321, 543, 654, 688
TscAI CASTG 2 cut(s) 137, 487
TseI GCWGC 4 cut(s) 269, 485, 590, 593
TspDTI ATGAA 4 cut(s) 224, 288, 533, 652
TspGWI ACGGA 2 cut(s) 370, 630
TspRI CASTG 2 cut(s) 137, 487
VspI ATTAAT 1 cut(s) 543
XapI RAATTY 1 cut(s) 343
XceI RCATGY 2 cut(s) 440, 515
XmaJI CCTAGG 1 cut(s) 257
XmiI GTMKAC 1 cut(s) 451
XspI CTAG 2 cut(s) 258, 531
ZrmI AGTACT 1 cut(s) 154
Zsp2I ATGCAT 1 cut(s) 661
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.