Prupe.8G088300_v2.0.a1

Protein SHI RELATED SEQUENCE

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp08
OG0009392
Physical Location & Seq
Forward (+)
12137883 .. 12139434
1552 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.8G088300.1

Sequence Viewer

Length: 705 bp
ATGGTGAAGCGAGGAGGAGGAGAAGGAGGAGTGATAATGGCTTCAAGATGCCAAGACTGTGGGAACCAAGCAAAGAAGGAGTGTGTGTACATGAGATGCAGGACTTGCTGTAAGAGCAAAGGGTTTCACTGCCAAACCCACGTCAAAAGCACTTGGGTTCCGGTCTACAGAAGACGCCAACAGCAGCTTCCTGCAGCAGCTCTTCTTTCTCAACAGCACCTTCAGCTTTATCATGAACAAGGACATCATCACCAAAACCCTAAGAGGCCAGCTGAACACCTCACCAATCCGTCTTCCTCAACAAGGCTAGATCAAGAGGTGAATTTTCCAGCTGAAGTGAATTCCATGGCAACTTTTAAGTGTGTTCGAGTGAGCTCAGTGGAAGACATGGTTGATCAGCACGCTTATCAGACAAGCGTGGTGATCGGAGGGCATGTATTTAAGGGAATTCTCTATGATCAAGGTCCTGATCATCATCATCAAAATTGTTACACCACTGTAGGCCAAAGCTCCAGCTCCCATCATGTATTGTTAAATAGGAAAACTAGTAATTTTATTAATGCTGCTGCAAATACTGCTTCCACTTCATCAGCTCCTGATCCTCCTGCAGATCAATTACCACATCATCATCCTCCTTCTTCATACCCATTGATTCCCTTTAATGCTTTCATGCCTGGTACGCAATTTTTCATTAACCCAAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF702

Protein Analysis

235

Amino Acids

26.06

Weight (kDa)

8.99

Isoelectric Point (pI)

46.4

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 165
AclWI GGATC 1 cut(s) 593
AcsI RAATTY 3 cut(s) 322, 340, 447
AcuI CTGAAG 2 cut(s) 206, 354
AcyI GRCGYC 1 cut(s) 175
AfaI GTAC 2 cut(s) 89, 679
AfiI CCNNNNNNNGG 1 cut(s) 303
AgsI TTSAA 1 cut(s) 45
AhlI ACTAGT 1 cut(s) 545
AjiI CACGTC 1 cut(s) 142
AjnI CCWGG 1 cut(s) 673
AjuI GAANNNNNNNTTGG 2 cut(s) 171, 203
AluBI AGCT 9 cut(s) 187, 200, 226, 272, 332, 375, 510, 516, 593
AluI AGCT 9 cut(s) 187, 200, 226, 272, 332, 375, 510, 516, 593
Alw21I GWGCWC 1 cut(s) 377
AlwI GGATC 1 cut(s) 593
AlwNI CAGNNNCTG 1 cut(s) 596
AoxI GGCC 2 cut(s) 266, 502
ApeKI GCWGC 5 cut(s) 184, 194, 197, 563, 566
ApoI RAATTY 3 cut(s) 322, 340, 447
AseI ATTAAT 1 cut(s) 558
AspS9I GGNCC 1 cut(s) 464
AsuHPI GGTGA 5 cut(s) 16, 242, 274, 331, 433
AvaII GGWCC 1 cut(s) 464
BanII GRGCYC 1 cut(s) 377
BbsI GAAGAC 3 cut(s) 178, 285, 390
Bbv12I GWGCWC 1 cut(s) 377
BbvI GCAGC 5 cut(s) 196, 206, 209, 550, 553
BccI CCATC 1 cut(s) 528
BciT130I CCWGG 1 cut(s) 675
BclI TGATCA 3 cut(s) 394, 457, 469
BcuI ACTAGT 1 cut(s) 545
BfaI CTAG 2 cut(s) 308, 546
BfmI CTRYAG 4 cut(s) 166, 192, 498, 606
BisI GCNGC 5 cut(s) 185, 195, 198, 564, 567
BlsI GCNGC 5 cut(s) 186, 196, 199, 565, 568
Bme1390I CCNGG 1 cut(s) 675
Bme18I GGWCC 1 cut(s) 464
BmgBI CACGTC 1 cut(s) 142
BmgT120I GGNCC 1 cut(s) 464
BmiI GGNNCC 2 cut(s) 65, 159
BmrFI CCNGG 1 cut(s) 675
BmsI GCATC 2 cut(s) 38, 86
BpiI GAAGAC 3 cut(s) 178, 285, 390
BpmI CTGGAG 1 cut(s) 496
BsaBI GATNNNNATC 1 cut(s) 474
BsaHI GRCGYC 1 cut(s) 175
BsaJI CCNNGG 1 cut(s) 345
BsaWI WCCGGW 1 cut(s) 160
BsaXI ACNNNNNCTCC 4 cut(s) 71, 101, 420, 450
Bsc4I CCNNNNNNNGG 1 cut(s) 303
Bse8I GATNNNNATC 1 cut(s) 474
BseBI CCWGG 1 cut(s) 675
BseDI CCNNGG 1 cut(s) 345
BseGI GGATG 1 cut(s) 628
BseJI GATNNNNATC 1 cut(s) 474
BseLI CCNNNNNNNGG 1 cut(s) 303
BseMII CTCAG 1 cut(s) 390
BseRI GAGGAG 4 cut(s) 27, 30, 33, 42
BseXI GCAGC 5 cut(s) 196, 206, 209, 550, 553
BshFI GGCC 2 cut(s) 268, 504
BsiHKAI GWGCWC 1 cut(s) 377
BsiSI CCGG 1 cut(s) 161
BslI CCNNNNNNNGG 1 cut(s) 303
BsnI GGCC 2 cut(s) 268, 504
Bsp1286I GDGCHC 1 cut(s) 377
Bsp1407I TGTACA 1 cut(s) 87
Bsp143I GATC 7 cut(s) 310, 394, 423, 457, 469, 598, 610
Bsp19I CCATGG 1 cut(s) 345
BspANI GGCC 2 cut(s) 268, 504
BspCNI CTCAG 1 cut(s) 389
BspHI TCATGA 1 cut(s) 232
BspLI GGNNCC 2 cut(s) 65, 159
BspMAI CTGCAG 2 cut(s) 196, 610
BspPI GGATC 1 cut(s) 593
BspQI GCTCTTC 1 cut(s) 207
BsrGI TGTACA 1 cut(s) 87
BssECI CCNNGG 1 cut(s) 345
BssMI GATC 7 cut(s) 310, 394, 423, 457, 469, 598, 610
BssNI GRCGYC 1 cut(s) 175
BssT1I CCWWGG 1 cut(s) 345
Bst2UI CCWGG 1 cut(s) 675
Bst4CI ACNGT 2 cut(s) 59, 499
Bst6I CTCTTC 1 cut(s) 207
BstACI GRCGYC 1 cut(s) 175
BstAPI GCANNNNNTGC 2 cut(s) 105, 575
BstAUI TGTACA 1 cut(s) 87
BstC8I GCNNGC 2 cut(s) 270, 402
BstDEI CTNAG 2 cut(s) 261, 376
BstDSI CCRYGG 1 cut(s) 345
BstENI CCTNNNNNAGG 1 cut(s) 301
BstF5I GGATG 1 cut(s) 628
BstKTI GATC 7 cut(s) 313, 397, 426, 460, 472, 601, 613
BstMBI GATC 7 cut(s) 310, 394, 423, 457, 469, 598, 610
BstMWI GCNNNNNNNGC 5 cut(s) 105, 114, 223, 575, 679
BstNI CCWGG 1 cut(s) 675
BstNSI RCATGY 1 cut(s) 437
BstSCI CCNGG 1 cut(s) 673
BstSFI CTRYAG 4 cut(s) 166, 192, 498, 606
BstV1I GCAGC 5 cut(s) 196, 206, 209, 550, 553
BstV2I GAAGAC 3 cut(s) 178, 285, 390
BstXI CCANNNNNNTGG 1 cut(s) 59
BsuRI GGCC 2 cut(s) 268, 504
BtgI CCRYGG 1 cut(s) 345
BtrI CACGTC 1 cut(s) 142
BtsCI GGATG 1 cut(s) 628
BtsI GCAGTG 1 cut(s) 127
BtsIMutI CAGTG 3 cut(s) 127, 384, 495
Cac8I GCNNGC 2 cut(s) 270, 402
CaiI CAGNNNCTG 1 cut(s) 596
CciI TCATGA 1 cut(s) 232
Cfr13I GGNCC 1 cut(s) 464
CseI GACGC 1 cut(s) 183
Csp6I GTAC 2 cut(s) 88, 678
CviAII CATG 7 cut(s) 91, 233, 346, 388, 434, 524, 670
CviQI GTAC 2 cut(s) 88, 678
DdeI CTNAG 2 cut(s) 261, 376
DpnI GATC 7 cut(s) 312, 396, 425, 459, 471, 600, 612
DpnII GATC 7 cut(s) 310, 394, 423, 457, 469, 598, 610
Eam1104I CTCTTC 1 cut(s) 207
EarI CTCTTC 1 cut(s) 207
Ecl136II GAGCTC 1 cut(s) 375
Eco130I CCWWGG 1 cut(s) 345
Eco24I GRGCYC 1 cut(s) 377
Eco47I GGWCC 1 cut(s) 464
Eco53kI GAGCTC 1 cut(s) 375
Eco57I CTGAAG 2 cut(s) 206, 354
EcoICRI GAGCTC 1 cut(s) 375
EcoNI CCTNNNNNAGG 1 cut(s) 301
EcoO109I RGGNCCY 1 cut(s) 464
EcoRI GAATTC 2 cut(s) 340, 447
EcoRII CCWGG 1 cut(s) 673
EcoT14I CCWWGG 1 cut(s) 345
EcoT38I GRGCYC 1 cut(s) 377
ErhI CCWWGG 1 cut(s) 345
FaeI CATG 7 cut(s) 94, 236, 349, 391, 437, 527, 673
FaiI YATR 9 cut(s) 92, 234, 347, 389, 435, 456, 525, 643, 671
FatI CATG 7 cut(s) 90, 232, 345, 387, 433, 523, 669
FbaI TGATCA 3 cut(s) 394, 457, 469
FblI GTMKAC 1 cut(s) 165
Fnu4HI GCNGC 5 cut(s) 185, 195, 198, 564, 567
FokI GGATG 1 cut(s) 615
FriOI GRGCYC 1 cut(s) 377
Fsp4HI GCNGC 5 cut(s) 185, 195, 198, 564, 567
FspBI CTAG 2 cut(s) 308, 546
GluI GCNGC 5 cut(s) 185, 195, 198, 564, 567
GsuI CTGGAG 1 cut(s) 496
HaeIII GGCC 2 cut(s) 268, 504
HapII CCGG 1 cut(s) 161
HgaI GACGC 1 cut(s) 183
Hin1I GRCGYC 1 cut(s) 175
Hin1II CATG 7 cut(s) 94, 236, 349, 391, 437, 527, 673
HinfI GANTC 1 cut(s) 652
HpaII CCGG 1 cut(s) 161
HphI GGTGA 5 cut(s) 16, 242, 274, 331, 433
Hpy166II GTNNAC 2 cut(s) 88, 166
Hpy188I TCNGA 2 cut(s) 411, 428
Hpy188III TCNNGA 5 cut(s) 45, 233, 314, 467, 596
Hpy8I GTNNAC 2 cut(s) 88, 166
HpyAV CCTTC 4 cut(s) 17, 70, 230, 645
HpyCH4III ACNGT 2 cut(s) 59, 499
HpyCH4IV ACGT 1 cut(s) 141
HpyCH4V TGCA 4 cut(s) 99, 194, 569, 608
HpyF10VI GCNNNNNNNGC 5 cut(s) 105, 114, 223, 575, 679
HpyF3I CTNAG 2 cut(s) 261, 376
HpySE526I ACGT 1 cut(s) 141
Hsp92I GRCGYC 1 cut(s) 175
Hsp92II CATG 7 cut(s) 94, 236, 349, 391, 437, 527, 673
Ksp22I TGATCA 3 cut(s) 394, 457, 469
Kzo9I GATC 7 cut(s) 310, 394, 423, 457, 469, 598, 610
LguI GCTCTTC 1 cut(s) 207
LmnI GCTCC 3 cut(s) 515, 521, 598
Lsp1109I GCAGC 5 cut(s) 196, 206, 209, 550, 553
LweI GCATC 2 cut(s) 38, 86
MaeI CTAG 2 cut(s) 308, 546
MaeII ACGT 1 cut(s) 141
MaeIII GTNAC 1 cut(s) 488
MalI GATC 7 cut(s) 312, 396, 425, 459, 471, 600, 612
MboI GATC 7 cut(s) 310, 394, 423, 457, 469, 598, 610
MboII GAAGA 5 cut(s) 183, 194, 285, 395, 630
MhlI GDGCHC 1 cut(s) 377
MluCI AATT 7 cut(s) 322, 340, 447, 484, 550, 614, 683
MseI TTAA 6 cut(s) 357, 441, 533, 558, 660, 693
MspA1I CMGCKG 2 cut(s) 272, 332
MspI CCGG 1 cut(s) 161
MspR9I CCNGG 1 cut(s) 675
MvaI CCWGG 1 cut(s) 675
MwoI GCNNNNNNNGC 5 cut(s) 105, 114, 223, 575, 679
NcoI CCATGG 1 cut(s) 345
NdeII GATC 7 cut(s) 310, 394, 423, 457, 469, 598, 610
NlaIII CATG 7 cut(s) 94, 236, 349, 391, 437, 527, 673
NlaIV GGNNCC 2 cut(s) 65, 159
NspI RCATGY 1 cut(s) 437
PagI TCATGA 1 cut(s) 232
PciSI GCTCTTC 1 cut(s) 207
PfeI GAWTC 1 cut(s) 652
PkrI GCNGC 5 cut(s) 186, 196, 199, 565, 568
PpuMI RGGWCCY 1 cut(s) 464
PshBI ATTAAT 1 cut(s) 558
Psp124BI GAGCTC 1 cut(s) 377
Psp5II RGGWCCY 1 cut(s) 464
Psp6I CCWGG 1 cut(s) 673
PspGI CCWGG 1 cut(s) 673
PspN4I GGNNCC 2 cut(s) 65, 159
PspPI GGNCC 1 cut(s) 464
PspPPI RGGWCCY 1 cut(s) 464
PstI CTGCAG 2 cut(s) 196, 610
PstNI CAGNNNCTG 1 cut(s) 596
PvuII CAGCTG 2 cut(s) 272, 332
RsaI GTAC 2 cut(s) 89, 679
RsaNI GTAC 2 cut(s) 88, 678
SacI GAGCTC 1 cut(s) 377
SapI GCTCTTC 1 cut(s) 207
SaqAI TTAA 6 cut(s) 357, 441, 533, 558, 660, 693
SatI GCNGC 5 cut(s) 185, 195, 198, 564, 567
Sau3AI GATC 7 cut(s) 310, 394, 423, 457, 469, 598, 610
Sau96I GGNCC 1 cut(s) 464
ScrFI CCNGG 1 cut(s) 675
SduI GDGCHC 1 cut(s) 377
SfaNI GCATC 2 cut(s) 38, 86
SfcI CTRYAG 4 cut(s) 166, 192, 498, 606
SinI GGWCC 1 cut(s) 464
SpeI ACTAGT 1 cut(s) 545
Sse9I AATT 7 cut(s) 322, 340, 447, 484, 550, 614, 683
SspMI CTAG 2 cut(s) 308, 546
SstI GAGCTC 1 cut(s) 377
StyD4I CCNGG 1 cut(s) 673
StyI CCWWGG 1 cut(s) 345
TaaI ACNGT 2 cut(s) 59, 499
TaiI ACGT 1 cut(s) 144
TaqI TCGA 1 cut(s) 367
TasI AATT 7 cut(s) 322, 340, 447, 484, 550, 614, 683
TatI WGTACW 1 cut(s) 87
TfiI GAWTC 1 cut(s) 652
Tru1I TTAA 6 cut(s) 357, 441, 533, 558, 660, 693
Tru9I TTAA 6 cut(s) 357, 441, 533, 558, 660, 693
TscAI CASTG 3 cut(s) 134, 384, 502
TseI GCWGC 5 cut(s) 184, 194, 197, 563, 566
TspDTI ATGAA 5 cut(s) 249, 576, 630, 658, 679
TspGWI ACGGA 1 cut(s) 279
TspRI CASTG 3 cut(s) 134, 384, 502
VpaK11BI GGWCC 1 cut(s) 464
VspI ATTAAT 1 cut(s) 558
XagI CCTNNNNNAGG 1 cut(s) 301
XapI RAATTY 3 cut(s) 322, 340, 447
XceI RCATGY 1 cut(s) 437
XmiI GTMKAC 1 cut(s) 165
XspI CTAG 2 cut(s) 308, 546
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.