Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0009560

Physical Location & Seq

Genomic Coordinates Forward (+)

24122934 .. 24126330

Gene Structure 3397 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1119500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1611 bp

ATGGGCAGCTCCGAAGACCACAACAAGATTCCCATCTCCTTCTACGACTCATCCTCTCCCTCCACCCCACTCCTCTCCAAACCCCCAATCGAAGACCCGCCACCGCCGCCATCCCCTCCGCAATCTTACGCCGACCCACCGGAATCCGATTCCGACCCGACCCAGTACCTCCAGATCTCCTACAACTACGGCCCCCGCCATTTCAAAGACCTCCCCTTTCTATTCCTCTTCCTCCTCTTCGTCCTCGCCACCTTCGCCATCGGAATCTTCGCCATTGTCCACCGCAACCAAAATTACTCCGTCCTCTCCTCCTTCGCCTACGACTCCGACTCCGCCTCCTGCGTCAAAAACGAAACTTTGACCCGTCTCCCCAACTGGGTCGGACCCACTTTCTCTTCTTCCTCGTCCTCTCTGCTCAAAGATTTGACATGGACCCTCGTGATTACGTTGATTCTAAGCCTGCCCATTTGCTTCAGTCTGCTAATTCTCCTCAAACACTACGCCAAGCAAGTCGTCTATATCTTCCTCCCCTTCTTCATCGTCTTCCCAATTTTCTTCAACGTGTACTGGTTCGTCGCCTGCACTCTCAGCTCGACCTGCAGCGACGCTCTCCCTTTAGCTTACAGAATTTTGATTCTGATTTTCGTGTTTTTGGTAATTGGGGTCGTTGTGTGGATTCTTGTGGTGAATTGGCACCGGATTGAGCTCACTGTGAGCATAATTGGGGTCGCTTCAGATGCGCTTTCGCAGAACTTGGGCTTGTTCGGGGTCCTGCCATGTTTGACATTCGGATTGGTGGTTTACTACGCACCCATTGTTGTGTTCTTGGTGTATGCCAGGTTCAATGGGAAGATTGAGCCGCGGGAATCGCAGGGAGTGTATAGTTGTGTGTGGAAGCAGGATGGTTGGGTGCCTGCTTATTACGCATTGGCGATTTTGACAATGTTGTGGTCGCTGACGACGATGGTGGAAGCTCAGGTGTATGTGATGAGTGGGACTGTTGCTCAGTGGTACTTTACTAAGGAGGATTCAACTCCGAGGCGCATTATTAGACGCTCGTTAAGAAATGCGTTTGGGCCCAACTTTGGCACAGTATGCCTATCTGGATTACTTTTATGTGTTGTTCGAGTTGTCCGTGCCATGGTTGATAGTGCAAAACAAGACGGTTCACCTGGGATGTTCACAATCAACTTTGCGGCAATAACTGGTGAAGCTTACTGCTCATCTGCAAAAATGACATATGAGCTTCTAAAGCGCAATCTTCTCTCTGCAGTTTTTGTGGAAACCATCTCCACTCGTCTATTGGCTGGAATTGTTTTTGTACTCTCAGCAATATATGCAATTGTGGTCTGCGCTATCTTACAAGCTGTGAGCAGTCTTGGGGTTGATTCGTACCTTGTGGCAGTTCTGGCGTGGGTGCTGCTGATCGTGATACTTGGTTTCTTTGTCCATGTGCTGGACAATGTAATTGACACCGTATACGTGTGCTATGCCATAGATAGGGATAGAGGGGAGGTTTGTAAACCGGAAGTTCACGAGGTTTACGTTCACTTACCCATTAGTCGAAACCATAGACCATCTTCTATGATCTCCAGAACTCTGGGTGTGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Choline_transpo

Protein Analysis

537

Amino Acids

59.71

Weight (kDa)

6.74

Isoelectric Point (pI)

49.8

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Choline_transpo	PF04515	214 - 386	1.9e-38	Plasma-membrane choline transporter
Choline_transpo	PF04515	395 - 502	2.3e-20	Plasma-membrane choline transporter

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009560)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G38640
fragaria_vesca	FvH4_2g01450 FvH4_2g01450
malus_domestica	MD05G1119500.v1.1 MD10G1123200.v1.1
prunus_persica	Prupe.8G163700_v2.0.a1 Prupe.8G163700_v2.0.a1
pyrus_communis	pycom05g11350 pycom10g10550
rosa_chinensis	RchiOBHm_Chr6g0244091
rosa_laevigata	RLG00000015383 RLG00000018378
rosa_multiflora	Rmu_co8225484.1_g000001 Rmu_sc0024622.1_g000001
rosa_roxburghii	Rroxscaffold_7G00216080
rosa_rugosa	Rorug05G0507300
rosa_samantha	Rh3BG287100 Rh6AG021800 Rh6BG017500 Rh6CG014200 Rh6DG015400
rosa_wichuraiana	Rw6G001790

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	605
AccB1I	GGYRCC	2 cut(s)	693, 910
AccB7I	CCANNNNNTGG	1 cut(s)	1456
AccI	GTMKAC	1 cut(s)	1479
AccII	CGCG	1 cut(s)	862
AcsI	RAATTY	1 cut(s)	627
AcuI	CTGAAG	2 cut(s)	457, 717
AfaI	GTAC	5 cut(s)	167, 566, 1013, 1323, 1394
AfiI	CCNNNNNNNGG	5 cut(s)	376, 1085, 1141, 1456, 1500
AflIII	ACRYGT	2 cut(s)	561, 1482
AgsI	TTSAA	4 cut(s)	205, 559, 844, 1032
AjnI	CCWGG	2 cut(s)	836, 1171
AluBI	AGCT	8 cut(s)	9, 591, 620, 706, 974, 1214, 1246, 1367
AluI	AGCT	8 cut(s)	9, 591, 620, 706, 974, 1214, 1246, 1367
Alw21I	GWGCWC	1 cut(s)	708
Alw26I	GTCTC	1 cut(s)	371
AoxI	GGCC	2 cut(s)	190, 1076
ApaI	GGGCCC	1 cut(s)	1080
ApeKI	GCWGC	3 cut(s)	6, 600, 1420
ApoI	RAATTY	1 cut(s)	627
AspLEI	GCGC	4 cut(s)	742, 1044, 1257, 1355
AspS9I	GGNCC	6 cut(s)	191, 383, 432, 769, 1076, 1077
AsuHPI	GGTGA	3 cut(s)	697, 1161, 1220
AvaII	GGWCC	3 cut(s)	383, 432, 769
BaeGI	GKGCMC	1 cut(s)	1080
BanI	GGYRCC	2 cut(s)	693, 910
BanII	GRGCYC	2 cut(s)	708, 1080
BauI	CACGAG	2 cut(s)	437, 1535
BbsI	GAAGAC	3 cut(s)	21, 99, 535
Bbv12I	GWGCWC	1 cut(s)	708
BbvI	GCAGC	3 cut(s)	18, 612, 1407
BccI	CCATC	7 cut(s)	41, 118, 266, 896, 958, 1295, 1585
BceAI	ACGGC	1 cut(s)	205
BciT130I	CCWGG	2 cut(s)	838, 1173
BcoDI	GTCTC	1 cut(s)	371
BfmI	CTRYAG	2 cut(s)	598, 1269
BfuAI	ACCTGC	1 cut(s)	605
BglII	AGATCT	1 cut(s)	174
BisI	GCNGC	6 cut(s)	7, 107, 601, 860, 1197, 1421
BlsI	GCNGC	6 cut(s)	8, 108, 602, 861, 1198, 1422
Bme1390I	CCNGG	2 cut(s)	838, 1173
Bme18I	GGWCC	3 cut(s)	383, 432, 769
BmgT120I	GGNCC	6 cut(s)	191, 383, 432, 769, 1076, 1077
BmiI	GGNNCC	7 cut(s)	193, 385, 434, 695, 770, 912, 1078
BmrFI	CCNGG	2 cut(s)	838, 1173
BmrI	ACTGGG	2 cut(s)	157, 385
BmsI	GCATC	1 cut(s)	727
BmuI	ACTGGG	2 cut(s)	157, 385
BpiI	GAAGAC	3 cut(s)	21, 99, 535
BpmI	CTGGAG	2 cut(s)	155, 1576
Bpu10I	CCTNAGC	1 cut(s)	975
BsaAI	YACGTR	1 cut(s)	1483
BsaBI	GATNNNNATC	1 cut(s)	32
BsaJI	CCNNGG	4 cut(s)	860, 1037, 1140, 1172
BsaWI	WCCGGW	3 cut(s)	139, 696, 1525
BsaXI	ACNNNNNCTCC	4 cut(s)	314, 320, 344, 350
Bsc4I	CCNNNNNNNGG	5 cut(s)	376, 1085, 1141, 1456, 1500
Bse1I	ACTGG	4 cut(s)	163, 380, 572, 1210
Bse8I	GATNNNNATC	1 cut(s)	32
BseBI	CCWGG	2 cut(s)	838, 1173
BseDI	CCNNGG	4 cut(s)	860, 1037, 1140, 1172
BseGI	GGATG	4 cut(s)	50, 110, 907, 1182
BseJI	GATNNNNATC	1 cut(s)	32
BseLI	CCNNNNNNNGG	5 cut(s)	376, 1085, 1141, 1456, 1500
BseMII	CTCAG	4 cut(s)	601, 989, 1019, 1341
BseNI	ACTGG	4 cut(s)	163, 380, 572, 1210
BseRI	GAGGAG	4 cut(s)	62, 224, 298, 479
BseSI	GKGCMC	1 cut(s)	1080
BseXI	GCAGC	3 cut(s)	18, 612, 1407
BsgI	GTGCAG	1 cut(s)	565
Bsh1236I	CGCG	1 cut(s)	862
BshFI	GGCC	2 cut(s)	192, 1078
BshNI	GGYRCC	2 cut(s)	693, 910
BsiHKAI	GWGCWC	1 cut(s)	708
BsiSI	CCGG	3 cut(s)	140, 697, 1526
BslFI	GGGAC	1 cut(s)	1009
BslI	CCNNNNNNNGG	5 cut(s)	376, 1085, 1141, 1456, 1500
BsmAI	GTCTC	1 cut(s)	371
BsmBI	CGTCTC	1 cut(s)	371
BsmFI	GGGAC	1 cut(s)	1009
BsnI	GGCC	2 cut(s)	192, 1078
Bsp120I	GGGCCC	1 cut(s)	1076
Bsp1286I	GDGCHC	2 cut(s)	708, 1080
Bsp143I	GATC	3 cut(s)	174, 1425, 1587
Bsp19I	CCATGG	1 cut(s)	1140
BspANI	GGCC	2 cut(s)	192, 1078
BspCNI	CTCAG	4 cut(s)	600, 988, 1018, 1340
BspFNI	CGCG	1 cut(s)	862
BspLI	GGNNCC	7 cut(s)	193, 385, 434, 695, 770, 912, 1078
BspMAI	CTGCAG	2 cut(s)	602, 1273
BspMI	ACCTGC	1 cut(s)	605
BspT107I	GGYRCC	2 cut(s)	693, 910
BsrI	ACTGG	4 cut(s)	163, 380, 572, 1210
BssECI	CCNNGG	4 cut(s)	860, 1037, 1140, 1172
BssMI	GATC	3 cut(s)	174, 1425, 1587
BssNAI	GTATAC	1 cut(s)	1480
BssSI	CACGAG	2 cut(s)	437, 1535
BssT1I	CCWWGG	1 cut(s)	1140
Bst1107I	GTATAC	1 cut(s)	1480
Bst2BI	CACGAG	2 cut(s)	437, 1535
Bst2UI	CCWGG	2 cut(s)	838, 1173
Bst4CI	ACNGT	5 cut(s)	712, 1000, 1093, 1166, 1477
Bst6I	CTCTTC	3 cut(s)	233, 242, 400
BstAPI	GCANNNNNTGC	2 cut(s)	1095, 1337
BstBAI	YACGTR	1 cut(s)	1483
BstC8I	GCNNGC	3 cut(s)	461, 580, 915
BstDEI	CTNAG	6 cut(s)	455, 587, 975, 1005, 1020, 1327
BstDSI	CCRYGG	2 cut(s)	860, 1140
BstF5I	GGATG	4 cut(s)	50, 110, 907, 1182
BstFNI	CGCG	1 cut(s)	862
BstHHI	GCGC	4 cut(s)	742, 1044, 1257, 1355
BstKTI	GATC	3 cut(s)	177, 1428, 1590
BstMAI	GTCTC	1 cut(s)	371
BstMBI	GATC	3 cut(s)	174, 1425, 1587
BstNI	CCWGG	2 cut(s)	838, 1173
BstSCI	CCNGG	2 cut(s)	836, 1171
BstSFI	CTRYAG	2 cut(s)	598, 1269
BstSLI	GKGCMC	1 cut(s)	1080
BstUI	CGCG	1 cut(s)	862
BstV1I	GCAGC	3 cut(s)	18, 612, 1407
BstV2I	GAAGAC	3 cut(s)	21, 99, 535
BstX2I	RGATCY	1 cut(s)	174
BstXI	CCANNNNNNTGG	1 cut(s)	1600
BstYI	RGATCY	1 cut(s)	174
BstZ17I	GTATAC	1 cut(s)	1480
BsuRI	GGCC	2 cut(s)	192, 1078
BtgI	CCRYGG	2 cut(s)	860, 1140
BtsCI	GGATG	4 cut(s)	50, 110, 907, 1182
BtsIMutI	CAGTG	2 cut(s)	708, 1013
BveI	ACCTGC	1 cut(s)	605
Cac8I	GCNNGC	3 cut(s)	461, 580, 915
CfoI	GCGC	4 cut(s)	742, 1044, 1257, 1355
Cfr13I	GGNCC	6 cut(s)	191, 383, 432, 769, 1076, 1077
Cfr42I	CCGCGG	1 cut(s)	863
CseI	GACGC	3 cut(s)	331, 614, 1062
Csp6I	GTAC	5 cut(s)	166, 565, 1012, 1322, 1393
CviAII	CATG	4 cut(s)	429, 777, 1141, 1451
CviQI	GTAC	5 cut(s)	166, 565, 1012, 1322, 1393
DdeI	CTNAG	6 cut(s)	455, 587, 975, 1005, 1020, 1327
DpnI	GATC	3 cut(s)	176, 1427, 1589
DpnII	GATC	3 cut(s)	174, 1425, 1587
Eam1104I	CTCTTC	3 cut(s)	233, 242, 400
EarI	CTCTTC	3 cut(s)	233, 242, 400
EciI	GGCGGA	1 cut(s)	322
Ecl136II	GAGCTC	1 cut(s)	706
Eco130I	CCWWGG	1 cut(s)	1140
Eco24I	GRGCYC	2 cut(s)	708, 1080
Eco47I	GGWCC	3 cut(s)	383, 432, 769
Eco53kI	GAGCTC	1 cut(s)	706
Eco57I	CTGAAG	2 cut(s)	457, 717
EcoICRI	GAGCTC	1 cut(s)	706
EcoO109I	RGGNCCY	1 cut(s)	769
EcoRII	CCWGG	2 cut(s)	836, 1171
EcoT14I	CCWWGG	1 cut(s)	1140
EcoT38I	GRGCYC	2 cut(s)	708, 1080
ErhI	CCWWGG	1 cut(s)	1140
Esp3I	CGTCTC	1 cut(s)	371
FaeI	CATG	4 cut(s)	432, 780, 1144, 1454
FaqI	GGGAC	1 cut(s)	1009
FatI	CATG	4 cut(s)	428, 776, 1140, 1450
FauI	CCCGC	3 cut(s)	105, 203, 855
FauNDI	CATATG	1 cut(s)	1240
FblI	GTMKAC	1 cut(s)	1479
Fnu4HI	GCNGC	6 cut(s)	7, 107, 601, 860, 1197, 1421
FokI	GGATG	4 cut(s)	37, 97, 914, 1189
FriOI	GRGCYC	2 cut(s)	708, 1080
Fsp4HI	GCNGC	6 cut(s)	7, 107, 601, 860, 1197, 1421
GlaI	GCGC	4 cut(s)	741, 1043, 1256, 1354
GluI	GCNGC	6 cut(s)	7, 107, 601, 860, 1197, 1421
GsuI	CTGGAG	2 cut(s)	155, 1576
HaeIII	GGCC	2 cut(s)	192, 1078
HapII	CCGG	3 cut(s)	140, 697, 1526
HgaI	GACGC	3 cut(s)	331, 614, 1062
HhaI	GCGC	4 cut(s)	742, 1044, 1257, 1355
Hin1II	CATG	4 cut(s)	432, 780, 1144, 1454
Hin6I	GCGC	4 cut(s)	740, 1042, 1255, 1353
HinP1I	GCGC	4 cut(s)	740, 1042, 1255, 1353
HindIII	AAGCTT	1 cut(s)	1212
HpaII	CCGG	3 cut(s)	140, 697, 1526
HphI	GGTGA	3 cut(s)	697, 1161, 1220
Hpy188III	TCNNGA	6 cut(s)	172, 439, 1104, 1429, 1535, 1593
Hpy99I	CGWCG	3 cut(s)	578, 608, 964
HpyAV	CCTTC	4 cut(s)	49, 262, 322, 541
HpyCH4III	ACNGT	5 cut(s)	712, 1000, 1093, 1166, 1477
HpyCH4IV	ACGT	4 cut(s)	446, 561, 1482, 1545
HpyCH4V	TGCA	6 cut(s)	582, 600, 1154, 1229, 1271, 1340
HpyF3I	CTNAG	6 cut(s)	455, 587, 975, 1005, 1020, 1327
HpySE526I	ACGT	4 cut(s)	446, 561, 1482, 1545
Hsp92II	CATG	4 cut(s)	432, 780, 1144, 1454
HspAI	GCGC	4 cut(s)	740, 1042, 1255, 1353
KspI	CCGCGG	1 cut(s)	863
Kzo9I	GATC	3 cut(s)	174, 1425, 1587
LmnI	GCTCC	1 cut(s)	14
Lsp1109I	GCAGC	3 cut(s)	18, 612, 1407
LweI	GCATC	1 cut(s)	727
MaeII	ACGT	4 cut(s)	446, 561, 1482, 1545
MalI	GATC	3 cut(s)	176, 1427, 1589
MboI	GATC	3 cut(s)	174, 1425, 1587
MfeI	CAATTG	1 cut(s)	1341
MflI	RGATCY	1 cut(s)	174
MhlI	GDGCHC	2 cut(s)	708, 1080
MlyI	GAGTC	3 cut(s)	41, 317, 323
MmeI	TCCRAC	3 cut(s)	177, 351, 361
MseI	TTAA	1 cut(s)	1061
MslI	CAYNNNNRTG	1 cut(s)	818
MspA1I	CMGCKG	1 cut(s)	862
MspI	CCGG	3 cut(s)	140, 697, 1526
MspR9I	CCNGG	2 cut(s)	838, 1173
MunI	CAATTG	1 cut(s)	1341
MvaI	CCWGG	2 cut(s)	838, 1173
MvnI	CGCG	1 cut(s)	862
NcoI	CCATGG	1 cut(s)	1140
NdeI	CATATG	1 cut(s)	1240
NdeII	GATC	3 cut(s)	174, 1425, 1587
NlaIII	CATG	4 cut(s)	432, 780, 1144, 1454
NlaIV	GGNNCC	7 cut(s)	193, 385, 434, 695, 770, 912, 1078
PcsI	WCGNNNNNNNCGW	3 cut(s)	959, 1132, 1542
PflMI	CCANNNNNTGG	1 cut(s)	1456
PkrI	GCNGC	6 cut(s)	8, 108, 602, 861, 1198, 1422
PleI	GAGTC	3 cut(s)	41, 317, 323
PpsI	GAGTC	3 cut(s)	41, 317, 323
Ppu21I	YACGTR	1 cut(s)	1483
PpuMI	RGGWCCY	1 cut(s)	769
Psp124BI	GAGCTC	1 cut(s)	708
Psp5II	RGGWCCY	1 cut(s)	769
Psp6I	CCWGG	2 cut(s)	836, 1171
PspGI	CCWGG	2 cut(s)	836, 1171
PspN4I	GGNNCC	7 cut(s)	193, 385, 434, 695, 770, 912, 1078
PspOMI	GGGCCC	1 cut(s)	1076
PspPI	GGNCC	6 cut(s)	191, 383, 432, 769, 1076, 1077
PspPPI	RGGWCCY	1 cut(s)	769
PsrI	GAACNNNNNNTAC	2 cut(s)	824, 856
PstI	CTGCAG	2 cut(s)	602, 1273
PsuI	RGATCY	1 cut(s)	174
RsaI	GTAC	5 cut(s)	167, 566, 1013, 1323, 1394
RsaNI	GTAC	5 cut(s)	166, 565, 1012, 1322, 1393
RseI	CAYNNNNRTG	1 cut(s)	818
SacI	GAGCTC	1 cut(s)	708
SacII	CCGCGG	1 cut(s)	863
SaqAI	TTAA	1 cut(s)	1061
SatI	GCNGC	6 cut(s)	7, 107, 601, 860, 1197, 1421
Sau3AI	GATC	3 cut(s)	174, 1425, 1587
Sau96I	GGNCC	6 cut(s)	191, 383, 432, 769, 1076, 1077
SchI	GAGTC	3 cut(s)	41, 317, 323
ScrFI	CCNGG	2 cut(s)	838, 1173
SduI	GDGCHC	2 cut(s)	708, 1080
SfaNI	GCATC	1 cut(s)	727
SfcI	CTRYAG	2 cut(s)	598, 1269
Sfr303I	CCGCGG	1 cut(s)	863
SgrBI	CCGCGG	1 cut(s)	863
SinI	GGWCC	3 cut(s)	383, 432, 769
SmiMI	CAYNNNNRTG	1 cut(s)	818
SstI	GAGCTC	1 cut(s)	708
StyD4I	CCNGG	2 cut(s)	836, 1171
StyI	CCWWGG	1 cut(s)	1140
TaaI	ACNGT	5 cut(s)	712, 1000, 1093, 1166, 1477
TaiI	ACGT	4 cut(s)	449, 564, 1485, 1548
TaqI	TCGA	4 cut(s)	90, 593, 1126, 1564
TatI	WGTACW	2 cut(s)	564, 1321
TauI	GCSGC	3 cut(s)	109, 862, 1199
Tru1I	TTAA	1 cut(s)	1061
Tru9I	TTAA	1 cut(s)	1061
TscAI	CASTG	2 cut(s)	715, 1013
TseI	GCWGC	3 cut(s)	6, 600, 1420
TspDTI	ATGAA	1 cut(s)	526
TspGWI	ACGGA	2 cut(s)	289, 1124
TspRI	CASTG	2 cut(s)	715, 1013
Van91I	CCANNNNNTGG	1 cut(s)	1456
VpaK11BI	GGWCC	3 cut(s)	383, 432, 769
XapI	RAATTY	1 cut(s)	627
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1300
XmiI	GTMKAC	1 cut(s)	1479

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.