Rw6G001790

Belongs to the CTL (choline transporter-like) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0009560
Physical Location & Seq
Reverse (-)
2815493 .. 2819001
3509 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G001790.1

Sequence Viewer

Length: 1605 bp
ATGAGCAGCCCCGAAGAACCCAGCAAGCCCATCTCCCTCTACGACTCGTCGTCCACTCCCCTCCTCTCCAAACCCGACGACCCGCCACCTCCTCCCTCCCCTTACTCCGACGAACCCGACTCCGACGCGACCCAGTACCTCCAGATCTCCTACAACCACGACCCGCGTCCCTTCAAGGACCTCCCTTTCTCCATCCTCTTCGTCCTCCTCGTCCTCGCCACCTTCGGCGTCGGAATCTTCGCCGTCTTCCACCGCAACGCCGACTACTCCAGCCTCTCCTCCTACGTATACGACTCCGATTCCACCTCCTGCGTCAACAACGCTTCTTTGACTCATGGCCTCGCCAACTGGGACCCTCTCAGTTCATCGTCATCTTTTCTAGAGGGTTTGACATGGACCCTAGTCATCACCTTAATCTTGAGCTTGCCCATTTGGTTCGGTCTGCTTTGGCTGCTCAAGAATTATGCTAAACAAGTCGTCTATGCTTCTCTCCCGTTCTTCATTTTGTTTCCGATTTTTTTCGACGTGTATTGGTTTGTTGCCTGCACAATCAGCTCAAGTTGCAGTGACTCTTTCCCTTTGGTTTACAGAATTTTGGTCCTGGTGTTTATTTTGTTAGTAATTGGAGTGATTTTGTGGATATTTGTGGCGAATCGGCATCGAATCGAGCTTACTATACAAATAATTGGGGTGGCATCGGATGCGCTTTCGAGGAATTTGGGTCTGTTTGGGGTTCTGCCTTTGTTGACTCTCGGGTTGGTGGTGTACTATGCACCCATTGTGGTGTTCTTGGTGTTTGCTAGGCTGAATGGGAAGATTGCGGCGGAAGAATCCGATGAGGTGTATAGTTGTGTGTGGAAGCAGGATTCTTGGGTCCCGGCTTATTATGCATTGGCGATTTTGACAATGTTGTGGTCTCTGACGATTATGGTGGAATCTCAGGTGTATGTGATTAGTGGGACTGTTGCTCAGTGGTACTTCACCAAGGAGGACTCGACTCCAAAGCGGGTTATTAGAAGTTCTTTGAGAAATGCCTTTGGCCCATCCTTTGGCACTGTGTGTCTATCTGGCTTACTTATATGTGTTGTTCGAATGGTGCGTACAATTGTTGATGGTGCAAGAAAAGAGGGTTCAGGCGGGATACTGAATCTTATGTTACGTTGCTGTGTTAATGCCTTGTTGTCAGCAATTGATTTTCTCAACAAGTTCACAATCATCTTTGCTGCAATAACTGGTGAATCTTACTGTTCATCTGCAAGGATGACGTATGAGCTTCTAAAACGTAATCTTCTTTCTGCGGTTTTTGTGGAAACCATCTCCACTCGACTTTTGGCTGGAATCATCTTCGTACTCTCAGCACTATATGCAATTGTGGCATGCGCTATCTTAAAGGCCGTGAGCAATCTTGGGGTTGATGCGTACTTTGTGGCTGCATTGGCCTGGGTGCTGCTGATCTTGGTACTGGGTTTCTTTGTCCATGTGCTGGATAATGTGATCGACACTGTGTACGTTTGTAAACAAGACGTTCATGAAGTTTATGTTAACTTACCAATTAGTAGAAATCATAGACCTTCATCTATGATCACCAGAACTCTGGGTGTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Choline_transpo

Protein Analysis

534

Amino Acids

59.19

Weight (kDa)

5.83

Isoelectric Point (pI)

45.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Choline_transpo PF04515 203 - 505 3.7e-70 Plasma-membrane choline transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 1049, 1483
AccI GTMKAC 1 cut(s) 288
AccII CGCG 2 cut(s) 128, 166
AciI CCGC 8 cut(s) 83, 164, 253, 821, 824, 1006, 1137, 1298
AcsI RAATTY 2 cut(s) 591, 715
AcyI GRCGYC 1 cut(s) 228
AdeI CACNNNGTG 1 cut(s) 1059
AfaI GTAC 8 cut(s) 137, 767, 977, 1102, 1350, 1421, 1461, 1508
AfiI CCNNNNNNNGG 2 cut(s) 1049, 1483
AflIII ACRYGT 1 cut(s) 525
AgsI TTSAA 1 cut(s) 175
AhdI GACNNNNNGTC 1 cut(s) 49
AjiI CACGTC 1 cut(s) 526
AjnI CCWGG 2 cut(s) 600, 1439
AluBI AGCT 4 cut(s) 423, 555, 670, 1273
AluI AGCT 4 cut(s) 423, 555, 670, 1273
Alw26I GTCTC 1 cut(s) 921
Ama87I CYCGRG 1 cut(s) 752
AoxI GGCC 4 cut(s) 337, 1039, 1392, 1437
ApeKI GCWGC 5 cut(s) 6, 451, 1223, 1430, 1447
ApoI RAATTY 2 cut(s) 591, 715
AspLEI GCGC 2 cut(s) 706, 1382
AspS9I GGNCC 6 cut(s) 178, 352, 396, 598, 874, 1040
AsuC2I CCSGG 1 cut(s) 878
AsuHPI GGTGA 4 cut(s) 400, 973, 1247, 1576
AsuII TTCGAA 1 cut(s) 1090
AvaI CYCGRG 1 cut(s) 752
AvaII GGWCC 5 cut(s) 178, 352, 396, 598, 874
BbsI GAAGAC 1 cut(s) 238
BbvI GCAGC 5 cut(s) 18, 438, 1210, 1417, 1434
BccI CCATC 5 cut(s) 38, 200, 1051, 1106, 1322
BceAI ACGGC 2 cut(s) 227, 1379
BciT130I CCWGG 2 cut(s) 602, 1441
BciVI GTATCC 1 cut(s) 1134
BclI TGATCA 1 cut(s) 1581
BcnI CCSGG 1 cut(s) 878
BcoDI GTCTC 1 cut(s) 921
BfaI CTAG 3 cut(s) 380, 401, 801
BfuI GTATCC 1 cut(s) 1134
BglII AGATCT 1 cut(s) 144
BisI GCNGC 6 cut(s) 7, 452, 822, 1224, 1431, 1448
BlsI GCNGC 6 cut(s) 8, 453, 823, 1225, 1432, 1449
Bme1390I CCNGG 3 cut(s) 602, 878, 1441
Bme18I GGWCC 5 cut(s) 178, 352, 396, 598, 874
BmeRI GACNNNNNGTC 1 cut(s) 49
BmeT110I CYCGRG 1 cut(s) 752
BmgBI CACGTC 1 cut(s) 526
BmgT120I GGNCC 6 cut(s) 178, 352, 396, 598, 874, 1040
BmiI GGNNCC 5 cut(s) 353, 354, 398, 875, 876
BmrFI CCNGG 3 cut(s) 602, 878, 1441
BmrI ACTGGG 3 cut(s) 127, 358, 1472
BmsI GCATC 4 cut(s) 667, 691, 704, 1405
BmuI ACTGGG 3 cut(s) 127, 358, 1472
BoxI GACNNNNGTC 2 cut(s) 165, 401
BpiI GAAGAC 1 cut(s) 238
BpmI CTGGAG 2 cut(s) 125, 253
Bpu14I TTCGAA 1 cut(s) 1090
BpuEI CTTGAG 3 cut(s) 439, 440, 541
BpuMI CCSGG 1 cut(s) 878
BsaAI YACGTR 1 cut(s) 286
BsaHI GRCGYC 1 cut(s) 228
BsaI GGTCTC 1 cut(s) 921
BsaJI CCNNGG 2 cut(s) 984, 1440
Bsc4I CCNNNNNNNGG 2 cut(s) 1049, 1483
Bse1I ACTGG 4 cut(s) 133, 353, 1237, 1467
BseBI CCWGG 2 cut(s) 602, 1441
BseDI CCNNGG 2 cut(s) 984, 1440
BseGI GGATG 4 cut(s) 192, 706, 1043, 1266
BseLI CCNNNNNNNGG 2 cut(s) 1049, 1483
BseMII CTCAG 4 cut(s) 373, 953, 983, 1368
BseNI ACTGG 4 cut(s) 133, 353, 1237, 1467
BseRI GAGGAG 4 cut(s) 53, 81, 197, 268
BseXI GCAGC 5 cut(s) 18, 438, 1210, 1417, 1434
BseYI CCCAGC 1 cut(s) 20
BsgI GTGCAG 1 cut(s) 529
Bsh1236I CGCG 2 cut(s) 128, 166
BshFI GGCC 4 cut(s) 339, 1041, 1394, 1439
BsiHKCI CYCGRG 1 cut(s) 752
BsiSI CCGG 1 cut(s) 878
BslFI GGGAC 4 cut(s) 153, 365, 860, 973
BslI CCNNNNNNNGG 2 cut(s) 1049, 1483
BsmAI GTCTC 1 cut(s) 921
BsmFI GGGAC 4 cut(s) 153, 365, 860, 973
BsnI GGCC 4 cut(s) 339, 1041, 1394, 1439
Bso31I GGTCTC 1 cut(s) 921
BsoBI CYCGRG 1 cut(s) 752
Bsp119I TTCGAA 1 cut(s) 1090
Bsp143I GATC 4 cut(s) 144, 1452, 1494, 1581
BspACI CCGC 8 cut(s) 83, 164, 253, 821, 824, 1006, 1137, 1298
BspANI GGCC 4 cut(s) 339, 1041, 1394, 1439
BspCNI CTCAG 4 cut(s) 372, 952, 982, 1367
BspFNI CGCG 2 cut(s) 128, 166
BspHI TCATGA 1 cut(s) 1528
BspLI GGNNCC 5 cut(s) 353, 354, 398, 875, 876
BspT104I TTCGAA 1 cut(s) 1090
BspTNI GGTCTC 1 cut(s) 921
BsrI ACTGG 4 cut(s) 133, 353, 1237, 1467
BssECI CCNNGG 2 cut(s) 984, 1440
BssMI GATC 4 cut(s) 144, 1452, 1494, 1581
BssNAI GTATAC 1 cut(s) 289
BssNI GRCGYC 1 cut(s) 228
BssT1I CCWWGG 1 cut(s) 984
Bst1107I GTATAC 1 cut(s) 289
Bst2UI CCWGG 2 cut(s) 602, 1441
Bst4CI ACNGT 4 cut(s) 964, 1057, 1247, 1504
Bst6I CTCTTC 1 cut(s) 203
BstACI GRCGYC 1 cut(s) 228
BstAPI GCANNNNNTGC 2 cut(s) 701, 1364
BstBAI YACGTR 1 cut(s) 286
BstBI TTCGAA 1 cut(s) 1090
BstC8I GCNNGC 4 cut(s) 26, 425, 544, 1378
BstDEI CTNAG 4 cut(s) 359, 939, 969, 1354
BstF5I GGATG 4 cut(s) 192, 706, 1043, 1266
BstFNI CGCG 2 cut(s) 128, 166
BstHHI GCGC 2 cut(s) 706, 1382
BstKTI GATC 4 cut(s) 147, 1455, 1497, 1584
BstMAI GTCTC 1 cut(s) 921
BstMBI GATC 4 cut(s) 144, 1452, 1494, 1581
BstMWI GCNNNNNNNGC 8 cut(s) 451, 552, 561, 701, 887, 1364, 1373, 1436
BstNI CCWGG 2 cut(s) 602, 1441
BstNSI RCATGY 1 cut(s) 1380
BstPAI GACNNNNGTC 2 cut(s) 165, 401
BstSCI CCNGG 3 cut(s) 600, 876, 1439
BstSNI TACGTA 1 cut(s) 286
BstUI CGCG 2 cut(s) 128, 166
BstV1I GCAGC 5 cut(s) 18, 438, 1210, 1417, 1434
BstV2I GAAGAC 1 cut(s) 238
BstX2I RGATCY 1 cut(s) 144
BstXI CCANNNNNNTGG 1 cut(s) 1594
BstYI RGATCY 1 cut(s) 144
BstZ17I GTATAC 1 cut(s) 289
BsuI GTATCC 1 cut(s) 1134
BsuRI GGCC 4 cut(s) 339, 1041, 1394, 1439
BtrI CACGTC 1 cut(s) 526
BtsCI GGATG 4 cut(s) 192, 706, 1043, 1266
BtsI GCAGTG 1 cut(s) 571
BtsIMutI CAGTG 4 cut(s) 571, 977, 1053, 1500
Cac8I GCNNGC 4 cut(s) 26, 425, 544, 1378
CciI TCATGA 1 cut(s) 1528
CfoI GCGC 2 cut(s) 706, 1382
Cfr13I GGNCC 6 cut(s) 178, 352, 396, 598, 874, 1040
CseI GACGC 4 cut(s) 134, 155, 217, 301
Csp6I GTAC 8 cut(s) 136, 766, 976, 1101, 1349, 1420, 1460, 1507
CviAII CATG 5 cut(s) 335, 393, 1377, 1478, 1529
CviQI GTAC 8 cut(s) 136, 766, 976, 1101, 1349, 1420, 1460, 1507
DdeI CTNAG 4 cut(s) 359, 939, 969, 1354
DpnI GATC 4 cut(s) 146, 1454, 1496, 1583
DpnII GATC 4 cut(s) 144, 1452, 1494, 1581
DraIII CACNNNGTG 1 cut(s) 1059
DriI GACNNNNNGTC 1 cut(s) 49
Eam1104I CTCTTC 1 cut(s) 203
Eam1105I GACNNNNNGTC 1 cut(s) 49
EarI CTCTTC 1 cut(s) 203
EciI GGCGGA 1 cut(s) 839
Eco105I TACGTA 1 cut(s) 286
Eco130I CCWWGG 1 cut(s) 984
Eco31I GGTCTC 1 cut(s) 921
Eco47I GGWCC 5 cut(s) 178, 352, 396, 598, 874
Eco88I CYCGRG 1 cut(s) 752
EcoO109I RGGNCCY 3 cut(s) 178, 352, 874
EcoRII CCWGG 2 cut(s) 600, 1439
EcoT14I CCWWGG 1 cut(s) 984
EcoT22I ATGCAT 1 cut(s) 892
ErhI CCWWGG 1 cut(s) 984
FaeI CATG 5 cut(s) 338, 396, 1380, 1481, 1532
FaqI GGGAC 4 cut(s) 153, 365, 860, 973
FatI CATG 5 cut(s) 334, 392, 1376, 1477, 1528
FauI CCCGC 4 cut(s) 90, 171, 999, 1130
FbaI TGATCA 1 cut(s) 1581
FblI GTMKAC 1 cut(s) 288
Fnu4HI GCNGC 6 cut(s) 7, 452, 822, 1224, 1431, 1448
FokI GGATG 4 cut(s) 179, 713, 1030, 1273
Fsp4HI GCNGC 6 cut(s) 7, 452, 822, 1224, 1431, 1448
FspBI CTAG 3 cut(s) 380, 401, 801
GlaI GCGC 2 cut(s) 705, 1381
GluI GCNGC 6 cut(s) 7, 452, 822, 1224, 1431, 1448
GsaI CCCAGC 1 cut(s) 24
GsuI CTGGAG 2 cut(s) 125, 253
HaeIII GGCC 4 cut(s) 339, 1041, 1394, 1439
HapII CCGG 1 cut(s) 878
HgaI GACGC 4 cut(s) 134, 155, 217, 301
HhaI GCGC 2 cut(s) 706, 1382
Hin1I GRCGYC 1 cut(s) 228
Hin1II CATG 5 cut(s) 338, 396, 1380, 1481, 1532
Hin6I GCGC 2 cut(s) 704, 1380
HinP1I GCGC 2 cut(s) 704, 1380
HincII GTYRAC 3 cut(s) 316, 747, 1543
HindII GTYRAC 3 cut(s) 316, 747, 1543
HpaI GTTAAC 1 cut(s) 1543
HpaII CCGG 1 cut(s) 878
HphI GGTGA 4 cut(s) 400, 973, 1247, 1576
Hpy188I TCNGA 8 cut(s) 109, 124, 233, 298, 513, 700, 835, 921
Hpy188III TCNNGA 5 cut(s) 142, 380, 418, 457, 1529
Hpy99I CGWCG 6 cut(s) 52, 80, 113, 128, 233, 527
HpyAV CCTTC 3 cut(s) 181, 232, 1581
HpyCH4III ACNGT 4 cut(s) 964, 1057, 1247, 1504
HpyCH4IV ACGT 7 cut(s) 285, 525, 1159, 1265, 1282, 1509, 1524
HpyCH4V TGCA 9 cut(s) 546, 564, 773, 890, 1118, 1226, 1256, 1367, 1433
HpyF10VI GCNNNNNNNGC 8 cut(s) 451, 552, 561, 701, 887, 1364, 1373, 1436
HpyF3I CTNAG 4 cut(s) 359, 939, 969, 1354
HpySE526I ACGT 7 cut(s) 285, 525, 1159, 1265, 1282, 1509, 1524
Hsp92I GRCGYC 1 cut(s) 228
Hsp92II CATG 5 cut(s) 338, 396, 1380, 1481, 1532
HspAI GCGC 2 cut(s) 704, 1380
KflI GGGWCCC 2 cut(s) 352, 874
Ksp22I TGATCA 1 cut(s) 1581
KspAI GTTAAC 1 cut(s) 1543
Kzo9I GATC 4 cut(s) 144, 1452, 1494, 1581
Lsp1109I GCAGC 5 cut(s) 18, 438, 1210, 1417, 1434
LweI GCATC 4 cut(s) 667, 691, 704, 1405
MaeI CTAG 3 cut(s) 380, 401, 801
MaeII ACGT 7 cut(s) 285, 525, 1159, 1265, 1282, 1509, 1524
MaeIII GTNAC 2 cut(s) 566, 1155
MalI GATC 4 cut(s) 146, 1454, 1496, 1583
MboI GATC 4 cut(s) 144, 1452, 1494, 1581
MboII GAAGA 9 cut(s) 26, 190, 229, 238, 490, 826, 839, 1280, 1336
MfeI CAATTG 3 cut(s) 1104, 1188, 1368
MflI RGATCY 1 cut(s) 144
MluCI AATT 9 cut(s) 460, 591, 621, 684, 715, 1104, 1188, 1368, 1551
MlyI GAGTC 8 cut(s) 38, 113, 287, 325, 563, 742, 986, 991
MmeI TCCRAC 3 cut(s) 132, 147, 211
Mph1103I ATGCAT 1 cut(s) 892
MseI TTAA 4 cut(s) 413, 1170, 1388, 1542
MslI CAYNNNNRTG 1 cut(s) 782
MspI CCGG 1 cut(s) 878
MspR9I CCNGG 3 cut(s) 602, 878, 1441
MunI CAATTG 3 cut(s) 1104, 1188, 1368
MvaI CCWGG 2 cut(s) 602, 1441
MvnI CGCG 2 cut(s) 128, 166
MwoI GCNNNNNNNGC 8 cut(s) 451, 552, 561, 701, 887, 1364, 1373, 1436
NciI CCSGG 1 cut(s) 878
NdeII GATC 4 cut(s) 144, 1452, 1494, 1581
NlaIII CATG 5 cut(s) 338, 396, 1380, 1481, 1532
NlaIV GGNNCC 5 cut(s) 353, 354, 398, 875, 876
NmuCI GTSAC 1 cut(s) 566
NsiI ATGCAT 1 cut(s) 892
NspI RCATGY 1 cut(s) 1380
NspV TTCGAA 1 cut(s) 1090
PaeI GCATGC 1 cut(s) 1380
PagI TCATGA 1 cut(s) 1528
PcsI WCGNNNNNNNCGW 2 cut(s) 207, 1096
PflMI CCANNNNNTGG 2 cut(s) 1049, 1483
PkrI GCNGC 6 cut(s) 8, 453, 823, 1225, 1432, 1449
PleI GAGTC 8 cut(s) 38, 113, 287, 325, 563, 742, 986, 991
PpsI GAGTC 8 cut(s) 38, 113, 287, 325, 563, 742, 986, 991
Ppu21I YACGTR 1 cut(s) 286
PpuMI RGGWCCY 3 cut(s) 178, 352, 874
PshAI GACNNNNGTC 2 cut(s) 165, 401
Psp5II RGGWCCY 3 cut(s) 178, 352, 874
Psp6I CCWGG 2 cut(s) 600, 1439
PspFI CCCAGC 1 cut(s) 20
PspGI CCWGG 2 cut(s) 600, 1439
PspN4I GGNNCC 5 cut(s) 353, 354, 398, 875, 876
PspPI GGNCC 6 cut(s) 178, 352, 396, 598, 874, 1040
PspPPI RGGWCCY 3 cut(s) 178, 352, 874
PsuI RGATCY 1 cut(s) 144
RsaI GTAC 8 cut(s) 137, 767, 977, 1102, 1350, 1421, 1461, 1508
RsaNI GTAC 8 cut(s) 136, 766, 976, 1101, 1349, 1420, 1460, 1507
RseI CAYNNNNRTG 1 cut(s) 782
SaqAI TTAA 4 cut(s) 413, 1170, 1388, 1542
SatI GCNGC 6 cut(s) 7, 452, 822, 1224, 1431, 1448
Sau3AI GATC 4 cut(s) 144, 1452, 1494, 1581
Sau96I GGNCC 6 cut(s) 178, 352, 396, 598, 874, 1040
SchI GAGTC 8 cut(s) 38, 113, 287, 325, 563, 742, 986, 991
ScrFI CCNGG 3 cut(s) 602, 878, 1441
SfaNI GCATC 4 cut(s) 667, 691, 704, 1405
SfuI TTCGAA 1 cut(s) 1090
SinI GGWCC 5 cut(s) 178, 352, 396, 598, 874
SmiMI CAYNNNNRTG 1 cut(s) 782
SmlI CTYRAG 3 cut(s) 418, 455, 556
SmoI CTYRAG 3 cut(s) 418, 455, 556
SnaBI TACGTA 1 cut(s) 286
SphI GCATGC 1 cut(s) 1380
Sse9I AATT 9 cut(s) 460, 591, 621, 684, 715, 1104, 1188, 1368, 1551
SsiI CCGC 8 cut(s) 83, 164, 253, 821, 824, 1006, 1137, 1298
SspMI CTAG 3 cut(s) 380, 401, 801
StyD4I CCNGG 3 cut(s) 600, 876, 1439
StyI CCWWGG 1 cut(s) 984
TaaI ACNGT 4 cut(s) 964, 1057, 1247, 1504
TaiI ACGT 7 cut(s) 288, 528, 1162, 1268, 1285, 1512, 1527
TaqI TCGA 8 cut(s) 522, 661, 666, 710, 995, 1090, 1324, 1497
TaqII GACCGA 1 cut(s) 428
TasI AATT 9 cut(s) 460, 591, 621, 684, 715, 1104, 1188, 1368, 1551
TatI WGTACW 1 cut(s) 765
TauI GCSGC 1 cut(s) 824
Tru1I TTAA 4 cut(s) 413, 1170, 1388, 1542
Tru9I TTAA 4 cut(s) 413, 1170, 1388, 1542
TscAI CASTG 4 cut(s) 571, 977, 1060, 1507
TseFI GTSAC 1 cut(s) 566
TseI GCWGC 5 cut(s) 6, 451, 1223, 1430, 1447
Tsp45I GTSAC 1 cut(s) 566
TspDTI ATGAA 6 cut(s) 354, 490, 1239, 1517, 1545, 1563
TspRI CASTG 4 cut(s) 571, 977, 1060, 1507
Van91I CCANNNNNTGG 2 cut(s) 1049, 1483
VpaK11BI GGWCC 5 cut(s) 178, 352, 396, 598, 874
XapI RAATTY 2 cut(s) 591, 715
XbaI TCTAGA 1 cut(s) 379
XceI RCATGY 1 cut(s) 1380
XcmI CCANNNNNNNNNTGG 1 cut(s) 1327
XmiI GTMKAC 1 cut(s) 288
XspI CTAG 3 cut(s) 380, 401, 801
Zsp2I ATGCAT 1 cut(s) 892
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.