Rh6AG021800

Belongs to the CTL (choline transporter-like) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0009560
Physical Location & Seq
Reverse (-)
2402037 .. 2405544
3508 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG021800.1

Sequence Viewer

Length: 1638 bp
ATGGGCAGCCCCGAAGAACCCAGCAAGCCCATCTCCCTCTACGACTCGTCGTCCACTCCCCTCCTCTCCAAACCCGACGACCCACCACCTCCTCCCTCCCCCTACTCCGACGAACCCGACTCCGACGCGGCCCAGTACCTCCAGATCTCCTACAACCACGACCCGCGTCCCTTCAAGGACCTCCCTTTCTCCATCCTCTTCGTCCTCCTCGTCCTCGCCACCTTCGGCGTCGGAATCTTCGCCGTCTTCCACCGCAACACCGACTACTCCAGCCTCTCCTCCTACGAATACGACTCCGATTCCACCTCCTGCGTCAAGAACGCTTCTTTGACTCATGGCCTCACCAACTGGGCGCCTCTCAGTTCATCGTCATCTTTTCTAAAGGGTCTGACATGGACCCTAGTCGTCACCTTAATCTTGAGCTTGCCCATTTGCTTCAGTCTGCTTTGGCTGCTCAAGAATTATGCTAAACAAGTCGTCTATGCTTCTCTCCCGTTCTTCATTTTGTTTCCGATTTTTTTCGACGTGTATTGGTTTGTTGCCTGCACAATCAGCTCAAGTTGCAGTGAATCTTTCCCTTTGGTTTACAGAATTTTGGTCCTGGTGTTTATTTTGTTAGTAATTGGAGTGATTTTGTGGATATTTGTGGCGAATCGGCATCGAATTGAGCTTACCATACAAATAATTGGGGTGGCATCGGATGCGCTTTCGAGGAATTTGGGTCTGTTTGGGGTTCTGCCTTTGTTGACTTTCGGGTTGGTGGTGTACTATGCACCCATTGTGGTGTTCTTGGTGTTTGCTAGGCTGAATGGGAAGATTGCGGTGGAAGAATCCGATGAGGTGTATAGTTGTGTGTGGAAGCAGGATTCTTGGGTCCCGGCTTATTATGCATTGGCGATTTTGACAATGTTGTGGTCTCTGACGATTATGGTGGAATCTCAGGTGTATGTGATTAGTGGGACTGTTGCTCAGTGGTACTTCACCAAGGAGGACTCAACTCCAAAGCGGGTTATTAGAAGTTCTTTGAGAAATGCCTTTGGCCCATCCTTTGGCACTGTGTGTCTATCTGGCTTACTTATATGTGTTGTTCGAATGGTACGTACAATTGTTGATGGTGCAAGAAAAGAGGGTTCAGGCGGGATACTGAATCTTATGTTACGTTGCTGTGTTAATGCCTTGCTGTCAGCAATTGATTTTCTCAACAAGTTCACAATCATCTTTGCTGCAATAACTGGTGAATCTTACTGTTCATCTGCAAGGATGACGTATGAGCTTCTAAAACGTAATCTTCTTTCTGCGGTTTTTGTGGAAACCATCTCCACTCGACTTTTGGCTGGAATCATCTTCGTACTCTCAGCACTATATGCAATTGTGGCATGCGCTATCTTAAAGGCCGTGAGCAATCTTGGGGTTGATGCGTACTTTGTGGCTGCATTGGCCTGGGTGCTGCTGATCTTGGTACTGGGTTTCTTTGTCCACGTGCTGGATAATGTGATCGACACTGTGTACGTGTGCTACGCCATAGATAGGGACAGAGGGGAAGTTTGTAAACAAGACGTTCATGAAGTTTATGTTAACTTACCAATTAGTAGAAATCATAGACCTTCATCTATGATCACCAGAACTCTGGGTGTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Choline_transpo

Protein Analysis

545

Amino Acids

60.48

Weight (kDa)

6.19

Isoelectric Point (pI)

43.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Choline_transpo PF04515 203 - 511 9.8e-73 Plasma-membrane choline transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 352
AccB7I CCANNNNNTGG 2 cut(s) 1049, 1483
AccII CGCG 2 cut(s) 128, 166
AciI CCGC 7 cut(s) 128, 164, 253, 821, 1006, 1137, 1298
AcsI RAATTY 2 cut(s) 591, 715
AcuI CTGAAG 1 cut(s) 421
AcvI CACGTG 1 cut(s) 1480
AcyI GRCGYC 2 cut(s) 228, 353
AdeI CACNNNGTG 1 cut(s) 1059
AfaI GTAC 9 cut(s) 137, 767, 977, 1098, 1102, 1350, 1421, 1461, 1508
AfiI CCNNNNNNNGG 3 cut(s) 1049, 1483, 1527
AflIII ACRYGT 2 cut(s) 525, 1509
AgsI TTSAA 1 cut(s) 175
AhdI GACNNNNNGTC 1 cut(s) 49
AjiI CACGTC 1 cut(s) 526
AjnI CCWGG 2 cut(s) 600, 1439
AluBI AGCT 4 cut(s) 423, 555, 670, 1273
AluI AGCT 4 cut(s) 423, 555, 670, 1273
Alw26I GTCTC 1 cut(s) 921
AoxI GGCC 5 cut(s) 129, 337, 1039, 1392, 1437
ApeKI GCWGC 5 cut(s) 6, 451, 1223, 1430, 1447
ApoI RAATTY 2 cut(s) 591, 715
AspLEI GCGC 3 cut(s) 355, 706, 1382
AspS9I GGNCC 6 cut(s) 130, 178, 396, 598, 874, 1040
AsuC2I CCSGG 1 cut(s) 878
AsuHPI GGTGA 5 cut(s) 334, 400, 973, 1247, 1609
AsuII TTCGAA 1 cut(s) 1090
AvaII GGWCC 4 cut(s) 178, 396, 598, 874
BanI GGYRCC 1 cut(s) 352
BbrPI CACGTG 1 cut(s) 1480
BbsI GAAGAC 1 cut(s) 238
BbvI GCAGC 5 cut(s) 18, 438, 1210, 1417, 1434
BccI CCATC 5 cut(s) 38, 200, 1051, 1106, 1322
BceAI ACGGC 2 cut(s) 227, 1379
BciT130I CCWGG 2 cut(s) 602, 1441
BciVI GTATCC 1 cut(s) 1134
BclI TGATCA 1 cut(s) 1614
BcnI CCSGG 1 cut(s) 878
BcoDI GTCTC 1 cut(s) 921
BfaI CTAG 2 cut(s) 401, 801
BfoI RGCGCY 1 cut(s) 356
BfuI GTATCC 1 cut(s) 1134
BglII AGATCT 1 cut(s) 144
BisI GCNGC 6 cut(s) 7, 129, 452, 1224, 1431, 1448
BlsI GCNGC 6 cut(s) 8, 130, 453, 1225, 1432, 1449
Bme1390I CCNGG 3 cut(s) 602, 878, 1441
Bme18I GGWCC 4 cut(s) 178, 396, 598, 874
BmeRI GACNNNNNGTC 1 cut(s) 49
BmgBI CACGTC 1 cut(s) 526
BmgT120I GGNCC 6 cut(s) 130, 178, 396, 598, 874, 1040
BmiI GGNNCC 4 cut(s) 354, 398, 875, 876
BmrFI CCNGG 3 cut(s) 602, 878, 1441
BmrI ACTGGG 3 cut(s) 127, 358, 1472
BmsI GCATC 4 cut(s) 667, 691, 704, 1405
BmuI ACTGGG 3 cut(s) 127, 358, 1472
BoxI GACNNNNGTC 2 cut(s) 165, 401
BpiI GAAGAC 1 cut(s) 238
BpmI CTGGAG 2 cut(s) 125, 253
Bpu14I TTCGAA 1 cut(s) 1090
BpuEI CTTGAG 3 cut(s) 439, 440, 541
BpuMI CCSGG 1 cut(s) 878
BsaAI YACGTR 3 cut(s) 1100, 1480, 1510
BsaHI GRCGYC 2 cut(s) 228, 353
BsaI GGTCTC 1 cut(s) 921
BsaJI CCNNGG 2 cut(s) 984, 1440
Bsc4I CCNNNNNNNGG 3 cut(s) 1049, 1483, 1527
Bse1I ACTGG 4 cut(s) 133, 353, 1237, 1467
BseBI CCWGG 2 cut(s) 602, 1441
BseDI CCNNGG 2 cut(s) 984, 1440
BseGI GGATG 4 cut(s) 192, 706, 1043, 1266
BseLI CCNNNNNNNGG 3 cut(s) 1049, 1483, 1527
BseMII CTCAG 4 cut(s) 373, 953, 983, 1368
BseNI ACTGG 4 cut(s) 133, 353, 1237, 1467
BseRI GAGGAG 4 cut(s) 53, 81, 197, 268
BseXI GCAGC 5 cut(s) 18, 438, 1210, 1417, 1434
BseYI CCCAGC 1 cut(s) 20
BsgI GTGCAG 1 cut(s) 529
Bsh1236I CGCG 2 cut(s) 128, 166
BshFI GGCC 5 cut(s) 131, 339, 1041, 1394, 1439
BshNI GGYRCC 1 cut(s) 352
BsiSI CCGG 1 cut(s) 878
BslFI GGGAC 4 cut(s) 153, 860, 973, 1544
BslI CCNNNNNNNGG 3 cut(s) 1049, 1483, 1527
BsmAI GTCTC 1 cut(s) 921
BsmFI GGGAC 4 cut(s) 153, 860, 973, 1544
BsnI GGCC 5 cut(s) 131, 339, 1041, 1394, 1439
Bso31I GGTCTC 1 cut(s) 921
Bsp119I TTCGAA 1 cut(s) 1090
Bsp143I GATC 4 cut(s) 144, 1452, 1494, 1614
BspACI CCGC 7 cut(s) 128, 164, 253, 821, 1006, 1137, 1298
BspANI GGCC 5 cut(s) 131, 339, 1041, 1394, 1439
BspCNI CTCAG 4 cut(s) 372, 952, 982, 1367
BspFNI CGCG 2 cut(s) 128, 166
BspHI TCATGA 1 cut(s) 1561
BspLI GGNNCC 4 cut(s) 354, 398, 875, 876
BspT104I TTCGAA 1 cut(s) 1090
BspT107I GGYRCC 1 cut(s) 352
BspTNI GGTCTC 1 cut(s) 921
BsrI ACTGG 4 cut(s) 133, 353, 1237, 1467
BssECI CCNNGG 2 cut(s) 984, 1440
BssMI GATC 4 cut(s) 144, 1452, 1494, 1614
BssNI GRCGYC 2 cut(s) 228, 353
BssT1I CCWWGG 1 cut(s) 984
Bst2UI CCWGG 2 cut(s) 602, 1441
Bst4CI ACNGT 4 cut(s) 964, 1057, 1247, 1504
Bst6I CTCTTC 1 cut(s) 203
BstACI GRCGYC 2 cut(s) 228, 353
BstAPI GCANNNNNTGC 2 cut(s) 701, 1364
BstBAI YACGTR 3 cut(s) 1100, 1480, 1510
BstBI TTCGAA 1 cut(s) 1090
BstC8I GCNNGC 4 cut(s) 26, 425, 544, 1378
BstDEI CTNAG 4 cut(s) 359, 939, 969, 1354
BstF5I GGATG 4 cut(s) 192, 706, 1043, 1266
BstFNI CGCG 2 cut(s) 128, 166
BstH2I RGCGCY 1 cut(s) 356
BstHHI GCGC 3 cut(s) 355, 706, 1382
BstKTI GATC 4 cut(s) 147, 1455, 1497, 1617
BstMAI GTCTC 1 cut(s) 921
BstMBI GATC 4 cut(s) 144, 1452, 1494, 1614
BstMWI GCNNNNNNNGC 8 cut(s) 451, 552, 561, 701, 887, 1364, 1373, 1436
BstNI CCWGG 2 cut(s) 602, 1441
BstNSI RCATGY 1 cut(s) 1380
BstPAI GACNNNNGTC 2 cut(s) 165, 401
BstSCI CCNGG 3 cut(s) 600, 876, 1439
BstSNI TACGTA 1 cut(s) 1100
BstUI CGCG 2 cut(s) 128, 166
BstV1I GCAGC 5 cut(s) 18, 438, 1210, 1417, 1434
BstV2I GAAGAC 1 cut(s) 238
BstX2I RGATCY 1 cut(s) 144
BstXI CCANNNNNNTGG 1 cut(s) 1627
BstYI RGATCY 1 cut(s) 144
BsuI GTATCC 1 cut(s) 1134
BsuRI GGCC 5 cut(s) 131, 339, 1041, 1394, 1439
BtrI CACGTC 1 cut(s) 526
BtsCI GGATG 4 cut(s) 192, 706, 1043, 1266
BtsI GCAGTG 1 cut(s) 571
BtsIMutI CAGTG 4 cut(s) 571, 977, 1053, 1500
Cac8I GCNNGC 4 cut(s) 26, 425, 544, 1378
CciI TCATGA 1 cut(s) 1561
CfoI GCGC 3 cut(s) 355, 706, 1382
Cfr13I GGNCC 6 cut(s) 130, 178, 396, 598, 874, 1040
CseI GACGC 4 cut(s) 134, 155, 217, 301
Csp6I GTAC 9 cut(s) 136, 766, 976, 1097, 1101, 1349, 1420, 1460, 1507
CviAII CATG 4 cut(s) 335, 393, 1377, 1562
CviQI GTAC 9 cut(s) 136, 766, 976, 1097, 1101, 1349, 1420, 1460, 1507
DdeI CTNAG 4 cut(s) 359, 939, 969, 1354
DinI GGCGCC 1 cut(s) 354
DpnI GATC 4 cut(s) 146, 1454, 1496, 1616
DpnII GATC 4 cut(s) 144, 1452, 1494, 1614
DraIII CACNNNGTG 1 cut(s) 1059
DriI GACNNNNNGTC 1 cut(s) 49
Eam1104I CTCTTC 1 cut(s) 203
Eam1105I GACNNNNNGTC 1 cut(s) 49
EarI CTCTTC 1 cut(s) 203
Eco105I TACGTA 1 cut(s) 1100
Eco130I CCWWGG 1 cut(s) 984
Eco31I GGTCTC 1 cut(s) 921
Eco47I GGWCC 4 cut(s) 178, 396, 598, 874
Eco57I CTGAAG 1 cut(s) 421
Eco72I CACGTG 1 cut(s) 1480
EcoO109I RGGNCCY 2 cut(s) 178, 874
EcoRII CCWGG 2 cut(s) 600, 1439
EcoT14I CCWWGG 1 cut(s) 984
EcoT22I ATGCAT 1 cut(s) 892
EgeI GGCGCC 1 cut(s) 354
EheI GGCGCC 1 cut(s) 354
ErhI CCWWGG 1 cut(s) 984
FaeI CATG 4 cut(s) 338, 396, 1380, 1565
FaqI GGGAC 4 cut(s) 153, 860, 973, 1544
FatI CATG 4 cut(s) 334, 392, 1376, 1561
FauI CCCGC 3 cut(s) 171, 999, 1130
FbaI TGATCA 1 cut(s) 1614
Fnu4HI GCNGC 6 cut(s) 7, 129, 452, 1224, 1431, 1448
FokI GGATG 4 cut(s) 179, 713, 1030, 1273
Fsp4HI GCNGC 6 cut(s) 7, 129, 452, 1224, 1431, 1448
FspBI CTAG 2 cut(s) 401, 801
GlaI GCGC 3 cut(s) 354, 705, 1381
GluI GCNGC 6 cut(s) 7, 129, 452, 1224, 1431, 1448
GsaI CCCAGC 1 cut(s) 24
GsuI CTGGAG 2 cut(s) 125, 253
HaeII RGCGCY 1 cut(s) 356
HaeIII GGCC 5 cut(s) 131, 339, 1041, 1394, 1439
HapII CCGG 1 cut(s) 878
HgaI GACGC 4 cut(s) 134, 155, 217, 301
HhaI GCGC 3 cut(s) 355, 706, 1382
Hin1I GRCGYC 2 cut(s) 228, 353
Hin1II CATG 4 cut(s) 338, 396, 1380, 1565
Hin6I GCGC 3 cut(s) 353, 704, 1380
HinP1I GCGC 3 cut(s) 353, 704, 1380
HincII GTYRAC 2 cut(s) 747, 1576
HindII GTYRAC 2 cut(s) 747, 1576
HpaI GTTAAC 1 cut(s) 1576
HpaII CCGG 1 cut(s) 878
HphI GGTGA 5 cut(s) 334, 400, 973, 1247, 1609
Hpy166II GTNNAC 9 cut(s) 54, 586, 747, 766, 1209, 1477, 1507, 1550, 1576
Hpy188I TCNGA 9 cut(s) 109, 124, 233, 298, 390, 513, 700, 835, 921
Hpy188III TCNNGA 5 cut(s) 142, 316, 418, 457, 1562
Hpy8I GTNNAC 9 cut(s) 54, 586, 747, 766, 1209, 1477, 1507, 1550, 1576
Hpy99I CGWCG 6 cut(s) 52, 80, 113, 128, 233, 527
HpyAV CCTTC 3 cut(s) 181, 232, 1614
HpyCH4III ACNGT 4 cut(s) 964, 1057, 1247, 1504
HpyCH4IV ACGT 8 cut(s) 525, 1099, 1159, 1265, 1282, 1479, 1509, 1557
HpyCH4V TGCA 9 cut(s) 546, 564, 773, 890, 1118, 1226, 1256, 1367, 1433
HpyF10VI GCNNNNNNNGC 8 cut(s) 451, 552, 561, 701, 887, 1364, 1373, 1436
HpyF3I CTNAG 4 cut(s) 359, 939, 969, 1354
HpySE526I ACGT 8 cut(s) 525, 1099, 1159, 1265, 1282, 1479, 1509, 1557
Hsp92I GRCGYC 2 cut(s) 228, 353
Hsp92II CATG 4 cut(s) 338, 396, 1380, 1565
HspAI GCGC 3 cut(s) 353, 704, 1380
KasI GGCGCC 1 cut(s) 352
KflI GGGWCCC 1 cut(s) 874
Ksp22I TGATCA 1 cut(s) 1614
KspAI GTTAAC 1 cut(s) 1576
Kzo9I GATC 4 cut(s) 144, 1452, 1494, 1614
Lsp1109I GCAGC 5 cut(s) 18, 438, 1210, 1417, 1434
LweI GCATC 4 cut(s) 667, 691, 704, 1405
MaeI CTAG 2 cut(s) 401, 801
MaeII ACGT 8 cut(s) 525, 1099, 1159, 1265, 1282, 1479, 1509, 1557
MaeIII GTNAC 2 cut(s) 406, 1155
MalI GATC 4 cut(s) 146, 1454, 1496, 1616
MboI GATC 4 cut(s) 144, 1452, 1494, 1614
MboII GAAGA 9 cut(s) 26, 190, 229, 238, 490, 826, 839, 1280, 1336
MfeI CAATTG 3 cut(s) 1104, 1188, 1368
MflI RGATCY 1 cut(s) 144
Mly113I GGCGCC 1 cut(s) 353
MlyI GAGTC 5 cut(s) 38, 113, 287, 325, 986
MmeI TCCRAC 3 cut(s) 132, 147, 211
Mph1103I ATGCAT 1 cut(s) 892
MseI TTAA 4 cut(s) 413, 1170, 1388, 1575
MslI CAYNNNNRTG 1 cut(s) 782
MspI CCGG 1 cut(s) 878
MspR9I CCNGG 3 cut(s) 602, 878, 1441
MunI CAATTG 3 cut(s) 1104, 1188, 1368
MvaI CCWGG 2 cut(s) 602, 1441
MvnI CGCG 2 cut(s) 128, 166
MwoI GCNNNNNNNGC 8 cut(s) 451, 552, 561, 701, 887, 1364, 1373, 1436
NarI GGCGCC 1 cut(s) 353
NciI CCSGG 1 cut(s) 878
NdeII GATC 4 cut(s) 144, 1452, 1494, 1614
NlaIII CATG 4 cut(s) 338, 396, 1380, 1565
NlaIV GGNNCC 4 cut(s) 354, 398, 875, 876
NmuCI GTSAC 1 cut(s) 406
NsiI ATGCAT 1 cut(s) 892
NspI RCATGY 1 cut(s) 1380
NspV TTCGAA 1 cut(s) 1090
PaeI GCATGC 1 cut(s) 1380
PagI TCATGA 1 cut(s) 1561
PcsI WCGNNNNNNNCGW 2 cut(s) 207, 1096
PflMI CCANNNNNTGG 2 cut(s) 1049, 1483
PkrI GCNGC 6 cut(s) 8, 130, 453, 1225, 1432, 1449
PleI GAGTC 5 cut(s) 38, 113, 287, 325, 986
PluTI GGCGCC 1 cut(s) 356
PmaCI CACGTG 1 cut(s) 1480
PmlI CACGTG 1 cut(s) 1480
PpsI GAGTC 5 cut(s) 38, 113, 287, 325, 986
Ppu21I YACGTR 3 cut(s) 1100, 1480, 1510
PpuMI RGGWCCY 2 cut(s) 178, 874
PshAI GACNNNNGTC 2 cut(s) 165, 401
Psp5II RGGWCCY 2 cut(s) 178, 874
Psp6I CCWGG 2 cut(s) 600, 1439
PspCI CACGTG 1 cut(s) 1480
PspFI CCCAGC 1 cut(s) 20
PspGI CCWGG 2 cut(s) 600, 1439
PspN4I GGNNCC 4 cut(s) 354, 398, 875, 876
PspPI GGNCC 6 cut(s) 130, 178, 396, 598, 874, 1040
PspPPI RGGWCCY 2 cut(s) 178, 874
PsuI RGATCY 1 cut(s) 144
RsaI GTAC 9 cut(s) 137, 767, 977, 1098, 1102, 1350, 1421, 1461, 1508
RsaNI GTAC 9 cut(s) 136, 766, 976, 1097, 1101, 1349, 1420, 1460, 1507
RseI CAYNNNNRTG 1 cut(s) 782
SaqAI TTAA 4 cut(s) 413, 1170, 1388, 1575
SatI GCNGC 6 cut(s) 7, 129, 452, 1224, 1431, 1448
Sau3AI GATC 4 cut(s) 144, 1452, 1494, 1614
Sau96I GGNCC 6 cut(s) 130, 178, 396, 598, 874, 1040
SchI GAGTC 5 cut(s) 38, 113, 287, 325, 986
ScrFI CCNGG 3 cut(s) 602, 878, 1441
SfaNI GCATC 4 cut(s) 667, 691, 704, 1405
SfoI GGCGCC 1 cut(s) 354
SfuI TTCGAA 1 cut(s) 1090
SinI GGWCC 4 cut(s) 178, 396, 598, 874
SmiMI CAYNNNNRTG 1 cut(s) 782
SmlI CTYRAG 3 cut(s) 418, 455, 556
SmoI CTYRAG 3 cut(s) 418, 455, 556
SnaBI TACGTA 1 cut(s) 1100
SphI GCATGC 1 cut(s) 1380
SsiI CCGC 7 cut(s) 128, 164, 253, 821, 1006, 1137, 1298
SspDI GGCGCC 1 cut(s) 352
SspMI CTAG 2 cut(s) 401, 801
StyD4I CCNGG 3 cut(s) 600, 876, 1439
StyI CCWWGG 1 cut(s) 984
TaaI ACNGT 4 cut(s) 964, 1057, 1247, 1504
TaiI ACGT 8 cut(s) 528, 1102, 1162, 1268, 1285, 1482, 1512, 1560
TaqI TCGA 6 cut(s) 522, 661, 710, 1090, 1324, 1497
TatI WGTACW 1 cut(s) 765
TauI GCSGC 1 cut(s) 131
Tru1I TTAA 4 cut(s) 413, 1170, 1388, 1575
Tru9I TTAA 4 cut(s) 413, 1170, 1388, 1575
TscAI CASTG 4 cut(s) 571, 977, 1060, 1507
TseFI GTSAC 1 cut(s) 406
TseI GCWGC 5 cut(s) 6, 451, 1223, 1430, 1447
Tsp45I GTSAC 1 cut(s) 406
TspDTI ATGAA 6 cut(s) 354, 490, 1239, 1550, 1578, 1596
TspRI CASTG 4 cut(s) 571, 977, 1060, 1507
Van91I CCANNNNNTGG 2 cut(s) 1049, 1483
VpaK11BI GGWCC 4 cut(s) 178, 396, 598, 874
XapI RAATTY 2 cut(s) 591, 715
XceI RCATGY 1 cut(s) 1380
XcmI CCANNNNNNNNNTGG 1 cut(s) 1327
XspI CTAG 2 cut(s) 401, 801
Zsp2I ATGCAT 1 cut(s) 892
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.