pycom05g11350

Belongs to the CTL (choline transporter-like) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0009560
Physical Location & Seq
Forward (+)
14891153 .. 14894374
3222 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g11350.1

Sequence Viewer

Length: 1728 bp
ATGGGCAGCTCCGAAGACCACAACAAGACGCCCACCTCCTTCTACGACTCATCCTCTCCCTCCACCCCACTCCTCTCCAAACCCCCAATCGAAGACCCGCCACCGCCGCCGTCCCCTCCGCCATCTTACGCCGACCCACCAGAATCCGATTCCGACCCGACCCAGTACCTCCAGATCTCCTACAACTACGGCCCCCGCCATTTCAAAGACCTCCCCTTTCTATTCCTCTTCCTCCTCTTCGTCCTCGCCACCTTCGCCATCGGAATCTTCGCCATTGTCCACCGCAACCAAAATTACTCCGCCCTATCCTCCTTCGCCTACGACTCCGACTCCGCCTCCTGCGTCAAAAACGAAACTTTGACCCGTCTCCCCAACTGGGTCGGACCCACTTTCTCTTCTTCCTCGTCCTCTCTGCTCAAAGATTTGACGTGGACCCTCGTGATTACGTTGATTCTAAGCCTGCCCATTTGCTTCAGTCTGCTAATTCTCCTCAAACACTACGCCAAGCAAGTCGTCTATATCTTCCTCCCCTTCTTCATCGTCTTCCCAATTTTCTTCAACGTGTACTGGTTCGTCGCCTGCACTCTCAGCTCGACCTGCAGCGACGCTCTCCCTTTAGCTTACAGAATTTTGATTCTGATTTTCGTGTTTTTGTTAATTGGGGTCGTTGTGTGGATTCTTGTGGTGAATTGGCACCGGATTGAGCTCACTGTGAGCATAATTGGGGTCGCTTCAGATGCGCTTTCGCGGAACTTGGGCTTGTTCGGGGTCCTGCCGTGTTTGACATTCGGATTGGTGGTTTACTACGCACCCATTGTTGTGTTCTTGGTGTATGCCAGGTTCAATGGGAAGATTGCGTCACGGGAATCGCAGGGAGCGTATAGTTGTGTGTGGAAGCAGGATGGTTGGGTGCCTGCTTATTACGCATTGGCGATTTTGACAATGTTGTGGTCGCTGACGACGATGGTGGAAGCTCAGGTGTATGTGATTAGTGGGACTGTTGCTCAGTGGTACTTTACTAAGGAGGATTCAACTCCGAGGCGCATTATAAGACGCTCGTTAAGAAATGCGTTTGGGCCCAACTTTGGCACAGTATGCCTATCTGGATTACTTTTATGTGTTGTTCGAGTTGTCCGTGCCATGGTTGATGGTGCAAGACAAGACGGTTCACCTGGGATGGTGTATTTTCTAATGCGCTGCTGTGTTAATGCCTTATTTTCGGCAATTGATTTTCTCAACAAGTTCACAATCAACTTTGCGGCAATAACTGGTGAAGCTTACTGCTCATCTGCAAAAATGACATATGAGCTTCTAAAGCGCAATCTTCTCTCCGCAGTTTTTGTGGAGACCATCTCCACTCGTCTATTGGCTGGAATTGTTTTTGTACTCTCAGCATTATATGCAATTGTGGTCTGCGCTATCTTACAAGCTGTGACCAGTCTTGGGGTTGATTCGTACTTTGTGGCAGTTCTGGCGTGGGTGCTGATGATCGTGATACTTGGTTTCTTTGTCCATGTGCTGGACAATGTAATTGACACCGTATACGTGTGCTATGCCATAGATAGGGATAGAGGGGAGGTTTGTAAACCGGAAGTTCACGAGGTTTACGTTCACTTACCCATTAGTCGAAACCATAGACCATCTTCTAGATCTCCAGAACTCTGGGTGTGTAAATTAATCTTTGAATTACAATCTTGTGGTGTTGATTTGTTCCTTGTATCAAGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Choline_transpo

Protein Analysis

576

Amino Acids

64.17

Weight (kDa)

7.06

Isoelectric Point (pI)

48.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Choline_transpo PF04515 214 - 523 2.2e-68 Plasma-membrane choline transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1049
Acc36I ACCTGC 1 cut(s) 605
AccB1I GGYRCC 2 cut(s) 693, 910
AccB7I CCANNNNNTGG 1 cut(s) 1519
AccI GTMKAC 1 cut(s) 1542
AccII CGCG 1 cut(s) 748
AcsI RAATTY 1 cut(s) 627
AcuI CTGAAG 2 cut(s) 457, 717
AcyI GRCGYC 1 cut(s) 29
AfaI GTAC 5 cut(s) 167, 566, 1013, 1386, 1457
AfiI CCNNNNNNNGG 6 cut(s) 376, 1085, 1141, 1443, 1519, 1563
AflIII ACRYGT 2 cut(s) 561, 1545
AgsI TTSAA 5 cut(s) 205, 559, 844, 1032, 1685
AjiI CACGTC 1 cut(s) 429
AjnI CCWGG 2 cut(s) 836, 1171
AluBI AGCT 8 cut(s) 9, 591, 620, 706, 974, 1277, 1309, 1430
AluI AGCT 8 cut(s) 9, 591, 620, 706, 974, 1277, 1309, 1430
Alw21I GWGCWC 1 cut(s) 708
Alw26I GTCTC 2 cut(s) 371, 1340
AoxI GGCC 2 cut(s) 190, 1076
ApaI GGGCCC 1 cut(s) 1080
ApeKI GCWGC 3 cut(s) 6, 600, 1197
ApoI RAATTY 1 cut(s) 627
AseI ATTAAT 1 cut(s) 1676
AspLEI GCGC 5 cut(s) 742, 1044, 1197, 1320, 1418
AspS9I GGNCC 6 cut(s) 191, 383, 432, 769, 1076, 1077
AsuHPI GGTGA 3 cut(s) 697, 1161, 1283
AvaII GGWCC 3 cut(s) 383, 432, 769
BaeGI GKGCMC 1 cut(s) 1080
BanI GGYRCC 2 cut(s) 693, 910
BanII GRGCYC 2 cut(s) 708, 1080
BauI CACGAG 2 cut(s) 437, 1598
BbsI GAAGAC 3 cut(s) 21, 99, 535
Bbv12I GWGCWC 1 cut(s) 708
BbvI GCAGC 3 cut(s) 18, 612, 1184
BccI CCATC 8 cut(s) 130, 266, 896, 958, 1142, 1171, 1358, 1648
BceAI ACGGC 3 cut(s) 94, 205, 760
BciT130I CCWGG 2 cut(s) 838, 1173
BcoDI GTCTC 2 cut(s) 371, 1340
BfaI CTAG 1 cut(s) 1647
BfmI CTRYAG 1 cut(s) 598
BfuAI ACCTGC 1 cut(s) 605
BglII AGATCT 2 cut(s) 174, 1649
BisI GCNGC 5 cut(s) 7, 107, 601, 1198, 1260
BlsI GCNGC 5 cut(s) 8, 108, 602, 1199, 1261
Bme1390I CCNGG 2 cut(s) 838, 1173
Bme18I GGWCC 3 cut(s) 383, 432, 769
BmgBI CACGTC 1 cut(s) 429
BmgT120I GGNCC 6 cut(s) 191, 383, 432, 769, 1076, 1077
BmiI GGNNCC 7 cut(s) 193, 385, 434, 695, 770, 912, 1078
BmrFI CCNGG 2 cut(s) 838, 1173
BmrI ACTGGG 2 cut(s) 157, 385
BmsI GCATC 1 cut(s) 727
BmuI ACTGGG 2 cut(s) 157, 385
BpiI GAAGAC 3 cut(s) 21, 99, 535
BplI GAGNNNNNCTC 2 cut(s) 1337, 1369
BpmI CTGGAG 2 cut(s) 155, 1638
Bpu10I CCTNAGC 1 cut(s) 975
BsaAI YACGTR 1 cut(s) 1546
BsaHI GRCGYC 1 cut(s) 29
BsaI GGTCTC 1 cut(s) 1340
BsaJI CCNNGG 3 cut(s) 1037, 1140, 1172
BsaWI WCCGGW 2 cut(s) 696, 1588
BsaXI ACNNNNNCTCC 4 cut(s) 314, 320, 344, 350
Bsc4I CCNNNNNNNGG 6 cut(s) 376, 1085, 1141, 1443, 1519, 1563
Bse1I ACTGG 5 cut(s) 163, 380, 572, 1273, 1437
BseBI CCWGG 2 cut(s) 838, 1173
BseDI CCNNGG 3 cut(s) 1037, 1140, 1172
BseGI GGATG 3 cut(s) 50, 907, 1182
BseLI CCNNNNNNNGG 6 cut(s) 376, 1085, 1141, 1443, 1519, 1563
BseMII CTCAG 4 cut(s) 601, 989, 1019, 1404
BseNI ACTGG 5 cut(s) 163, 380, 572, 1273, 1437
BseRI GAGGAG 3 cut(s) 62, 224, 479
BseSI GKGCMC 1 cut(s) 1080
BseXI GCAGC 3 cut(s) 18, 612, 1184
BsgI GTGCAG 1 cut(s) 565
Bsh1236I CGCG 1 cut(s) 748
BshFI GGCC 2 cut(s) 192, 1078
BshNI GGYRCC 2 cut(s) 693, 910
BsiHKAI GWGCWC 1 cut(s) 708
BsiSI CCGG 2 cut(s) 697, 1589
BslFI GGGAC 2 cut(s) 97, 1009
BslI CCNNNNNNNGG 6 cut(s) 376, 1085, 1141, 1443, 1519, 1563
BsmAI GTCTC 2 cut(s) 371, 1340
BsmBI CGTCTC 1 cut(s) 371
BsmFI GGGAC 2 cut(s) 97, 1009
BsnI GGCC 2 cut(s) 192, 1078
Bso31I GGTCTC 1 cut(s) 1340
Bsp120I GGGCCC 1 cut(s) 1076
Bsp1286I GDGCHC 2 cut(s) 708, 1080
Bsp143I GATC 3 cut(s) 174, 1488, 1649
Bsp19I CCATGG 1 cut(s) 1140
BspANI GGCC 2 cut(s) 192, 1078
BspCNI CTCAG 4 cut(s) 600, 988, 1018, 1403
BspFNI CGCG 1 cut(s) 748
BspLI GGNNCC 7 cut(s) 193, 385, 434, 695, 770, 912, 1078
BspMAI CTGCAG 1 cut(s) 602
BspMI ACCTGC 1 cut(s) 605
BspT107I GGYRCC 2 cut(s) 693, 910
BspTNI GGTCTC 1 cut(s) 1340
BsrI ACTGG 5 cut(s) 163, 380, 572, 1273, 1437
BssECI CCNNGG 3 cut(s) 1037, 1140, 1172
BssMI GATC 3 cut(s) 174, 1488, 1649
BssNAI GTATAC 1 cut(s) 1543
BssNI GRCGYC 1 cut(s) 29
BssSI CACGAG 2 cut(s) 437, 1598
BssT1I CCWWGG 1 cut(s) 1140
Bst1107I GTATAC 1 cut(s) 1543
Bst2BI CACGAG 2 cut(s) 437, 1598
Bst2UI CCWGG 2 cut(s) 838, 1173
Bst4CI ACNGT 5 cut(s) 712, 1000, 1093, 1166, 1540
Bst6I CTCTTC 3 cut(s) 233, 242, 400
BstACI GRCGYC 1 cut(s) 29
BstAPI GCANNNNNTGC 2 cut(s) 1095, 1400
BstBAI YACGTR 1 cut(s) 1546
BstC8I GCNNGC 3 cut(s) 461, 580, 915
BstDEI CTNAG 6 cut(s) 455, 587, 975, 1005, 1020, 1390
BstDSI CCRYGG 1 cut(s) 1140
BstF5I GGATG 3 cut(s) 50, 907, 1182
BstFNI CGCG 1 cut(s) 748
BstHHI GCGC 5 cut(s) 742, 1044, 1197, 1320, 1418
BstKTI GATC 3 cut(s) 177, 1491, 1652
BstMAI GTCTC 2 cut(s) 371, 1340
BstMBI GATC 3 cut(s) 174, 1488, 1649
BstNI CCWGG 2 cut(s) 838, 1173
BstSCI CCNGG 2 cut(s) 836, 1171
BstSFI CTRYAG 1 cut(s) 598
BstSLI GKGCMC 1 cut(s) 1080
BstUI CGCG 1 cut(s) 748
BstV1I GCAGC 3 cut(s) 18, 612, 1184
BstV2I GAAGAC 3 cut(s) 21, 99, 535
BstX2I RGATCY 2 cut(s) 174, 1649
BstXI CCANNNNNNTGG 1 cut(s) 1662
BstYI RGATCY 2 cut(s) 174, 1649
BstZ17I GTATAC 1 cut(s) 1543
BsuRI GGCC 2 cut(s) 192, 1078
BtgI CCRYGG 1 cut(s) 1140
BtrI CACGTC 1 cut(s) 429
BtsCI GGATG 3 cut(s) 50, 907, 1182
BtsIMutI CAGTG 2 cut(s) 708, 1013
BveI ACCTGC 1 cut(s) 605
Cac8I GCNNGC 3 cut(s) 461, 580, 915
CfoI GCGC 5 cut(s) 742, 1044, 1197, 1320, 1418
Cfr13I GGNCC 6 cut(s) 191, 383, 432, 769, 1076, 1077
CseI GACGC 5 cut(s) 37, 331, 614, 846, 1062
Csp6I GTAC 5 cut(s) 166, 565, 1012, 1385, 1456
CviAII CATG 2 cut(s) 1141, 1514
CviQI GTAC 5 cut(s) 166, 565, 1012, 1385, 1456
DdeI CTNAG 6 cut(s) 455, 587, 975, 1005, 1020, 1390
DpnI GATC 3 cut(s) 176, 1490, 1651
DpnII GATC 3 cut(s) 174, 1488, 1649
Eam1104I CTCTTC 3 cut(s) 233, 242, 400
EarI CTCTTC 3 cut(s) 233, 242, 400
EciI GGCGGA 3 cut(s) 108, 289, 322
Ecl136II GAGCTC 1 cut(s) 706
Eco130I CCWWGG 1 cut(s) 1140
Eco24I GRGCYC 2 cut(s) 708, 1080
Eco31I GGTCTC 1 cut(s) 1340
Eco47I GGWCC 3 cut(s) 383, 432, 769
Eco53kI GAGCTC 1 cut(s) 706
Eco57I CTGAAG 2 cut(s) 457, 717
EcoICRI GAGCTC 1 cut(s) 706
EcoO109I RGGNCCY 1 cut(s) 769
EcoRII CCWGG 2 cut(s) 836, 1171
EcoT14I CCWWGG 1 cut(s) 1140
EcoT38I GRGCYC 2 cut(s) 708, 1080
ErhI CCWWGG 1 cut(s) 1140
Esp3I CGTCTC 1 cut(s) 371
FaeI CATG 2 cut(s) 1144, 1517
FaqI GGGAC 2 cut(s) 97, 1009
FatI CATG 2 cut(s) 1140, 1513
FauI CCCGC 2 cut(s) 105, 203
FauNDI CATATG 1 cut(s) 1303
FblI GTMKAC 1 cut(s) 1542
Fnu4HI GCNGC 5 cut(s) 7, 107, 601, 1198, 1260
FokI GGATG 3 cut(s) 37, 914, 1189
FriOI GRGCYC 2 cut(s) 708, 1080
Fsp4HI GCNGC 5 cut(s) 7, 107, 601, 1198, 1260
FspBI CTAG 1 cut(s) 1647
GlaI GCGC 5 cut(s) 741, 1043, 1196, 1319, 1417
GluI GCNGC 5 cut(s) 7, 107, 601, 1198, 1260
GsuI CTGGAG 2 cut(s) 155, 1638
HaeIII GGCC 2 cut(s) 192, 1078
HapII CCGG 2 cut(s) 697, 1589
HgaI GACGC 5 cut(s) 37, 331, 614, 846, 1062
HhaI GCGC 5 cut(s) 742, 1044, 1197, 1320, 1418
Hin1I GRCGYC 1 cut(s) 29
Hin1II CATG 2 cut(s) 1144, 1517
Hin6I GCGC 5 cut(s) 740, 1042, 1195, 1318, 1416
HinP1I GCGC 5 cut(s) 740, 1042, 1195, 1318, 1416
HindIII AAGCTT 1 cut(s) 1275
HpaII CCGG 2 cut(s) 697, 1589
HphI GGTGA 3 cut(s) 697, 1161, 1283
Hpy188III TCNNGA 7 cut(s) 172, 439, 1104, 1492, 1598, 1647, 1655
Hpy99I CGWCG 3 cut(s) 578, 608, 964
HpyAV CCTTC 4 cut(s) 49, 262, 322, 541
HpyCH4III ACNGT 5 cut(s) 712, 1000, 1093, 1166, 1540
HpyCH4IV ACGT 5 cut(s) 428, 446, 561, 1545, 1608
HpyCH4V TGCA 5 cut(s) 582, 600, 1154, 1292, 1403
HpyF3I CTNAG 6 cut(s) 455, 587, 975, 1005, 1020, 1390
HpySE526I ACGT 5 cut(s) 428, 446, 561, 1545, 1608
Hsp92I GRCGYC 1 cut(s) 29
Hsp92II CATG 2 cut(s) 1144, 1517
HspAI GCGC 5 cut(s) 740, 1042, 1195, 1318, 1416
Kzo9I GATC 3 cut(s) 174, 1488, 1649
LmnI GCTCC 2 cut(s) 14, 875
Lsp1109I GCAGC 3 cut(s) 18, 612, 1184
LweI GCATC 1 cut(s) 727
MaeI CTAG 1 cut(s) 1647
MaeII ACGT 5 cut(s) 428, 446, 561, 1545, 1608
MaeIII GTNAC 2 cut(s) 858, 1432
MalI GATC 3 cut(s) 176, 1490, 1651
MboI GATC 3 cut(s) 174, 1488, 1649
MfeI CAATTG 2 cut(s) 1224, 1404
MflI RGATCY 2 cut(s) 174, 1649
MhlI GDGCHC 2 cut(s) 708, 1080
MlyI GAGTC 3 cut(s) 41, 317, 323
MmeI TCCRAC 3 cut(s) 177, 351, 361
MseI TTAA 4 cut(s) 656, 1061, 1206, 1676
MslI CAYNNNNRTG 1 cut(s) 818
MspI CCGG 2 cut(s) 697, 1589
MspR9I CCNGG 2 cut(s) 838, 1173
MunI CAATTG 2 cut(s) 1224, 1404
MvaI CCWGG 2 cut(s) 838, 1173
MvnI CGCG 1 cut(s) 748
NcoI CCATGG 1 cut(s) 1140
NdeI CATATG 1 cut(s) 1303
NdeII GATC 3 cut(s) 174, 1488, 1649
NlaIII CATG 2 cut(s) 1144, 1517
NlaIV GGNNCC 7 cut(s) 193, 385, 434, 695, 770, 912, 1078
NmuCI GTSAC 2 cut(s) 858, 1432
PcsI WCGNNNNNNNCGW 4 cut(s) 875, 959, 1132, 1605
PfeI GAWTC 9 cut(s) 143, 149, 264, 451, 634, 676, 866, 1028, 1451
PflMI CCANNNNNTGG 1 cut(s) 1519
PkrI GCNGC 5 cut(s) 8, 108, 602, 1199, 1261
PleI GAGTC 3 cut(s) 41, 317, 323
PpsI GAGTC 3 cut(s) 41, 317, 323
Ppu21I YACGTR 1 cut(s) 1546
PpuMI RGGWCCY 1 cut(s) 769
PshBI ATTAAT 1 cut(s) 1676
PsiI TTATAA 1 cut(s) 1049
Psp124BI GAGCTC 1 cut(s) 708
Psp5II RGGWCCY 1 cut(s) 769
Psp6I CCWGG 2 cut(s) 836, 1171
PspGI CCWGG 2 cut(s) 836, 1171
PspN4I GGNNCC 7 cut(s) 193, 385, 434, 695, 770, 912, 1078
PspOMI GGGCCC 1 cut(s) 1076
PspPI GGNCC 6 cut(s) 191, 383, 432, 769, 1076, 1077
PspPPI RGGWCCY 1 cut(s) 769
PsrI GAACNNNNNNTAC 2 cut(s) 824, 856
PstI CTGCAG 1 cut(s) 602
PsuI RGATCY 2 cut(s) 174, 1649
RsaI GTAC 5 cut(s) 167, 566, 1013, 1386, 1457
RsaNI GTAC 5 cut(s) 166, 565, 1012, 1385, 1456
RseI CAYNNNNRTG 1 cut(s) 818
SacI GAGCTC 1 cut(s) 708
SaqAI TTAA 4 cut(s) 656, 1061, 1206, 1676
SatI GCNGC 5 cut(s) 7, 107, 601, 1198, 1260
Sau3AI GATC 3 cut(s) 174, 1488, 1649
Sau96I GGNCC 6 cut(s) 191, 383, 432, 769, 1076, 1077
SchI GAGTC 3 cut(s) 41, 317, 323
ScrFI CCNGG 2 cut(s) 838, 1173
SduI GDGCHC 2 cut(s) 708, 1080
SfaNI GCATC 1 cut(s) 727
SfcI CTRYAG 1 cut(s) 598
SinI GGWCC 3 cut(s) 383, 432, 769
SmiMI CAYNNNNRTG 1 cut(s) 818
SspMI CTAG 1 cut(s) 1647
SstI GAGCTC 1 cut(s) 708
StyD4I CCNGG 2 cut(s) 836, 1171
StyI CCWWGG 1 cut(s) 1140
TaaI ACNGT 5 cut(s) 712, 1000, 1093, 1166, 1540
TaiI ACGT 5 cut(s) 431, 449, 564, 1548, 1611
TaqI TCGA 4 cut(s) 90, 593, 1126, 1627
TatI WGTACW 2 cut(s) 564, 1384
TauI GCSGC 2 cut(s) 109, 1262
TfiI GAWTC 9 cut(s) 143, 149, 264, 451, 634, 676, 866, 1028, 1451
Tru1I TTAA 4 cut(s) 656, 1061, 1206, 1676
Tru9I TTAA 4 cut(s) 656, 1061, 1206, 1676
TscAI CASTG 2 cut(s) 715, 1013
TseFI GTSAC 2 cut(s) 858, 1432
TseI GCWGC 3 cut(s) 6, 600, 1197
Tsp45I GTSAC 2 cut(s) 858, 1432
TspDTI ATGAA 1 cut(s) 526
TspGWI ACGGA 1 cut(s) 1124
TspRI CASTG 2 cut(s) 715, 1013
Van91I CCANNNNNTGG 1 cut(s) 1519
VpaK11BI GGWCC 3 cut(s) 383, 432, 769
VspI ATTAAT 1 cut(s) 1676
XapI RAATTY 1 cut(s) 627
XbaI TCTAGA 1 cut(s) 1646
XcmI CCANNNNNNNNNTGG 1 cut(s) 1363
XmiI GTMKAC 1 cut(s) 1542
XspI CTAG 1 cut(s) 1647
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.