MD10G1123200.v1.1

Belongs to the CTL (choline transporter-like) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0009560
Physical Location & Seq
Forward (+)
20058144 .. 20061557
3414 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1123200.v1.1.491

Sequence Viewer

Length: 1665 bp
ATGGGCAGCTCCGAAGAACCCAACAAGCCCATCTTCTACGACTCATCCTCTCCCTCCACCCCCCTCCTCTCCAAACCCCCAATCGAAAACCCACCACCGCCACCGTCCCCTCCGCCATCTTACGCCGACCCACCGGAATCCGATGCCGACCCGACCCAGTACCTCCAGATCTCCTACAACCACGGCCCCCGCCATTTCAAAGACCTCCCCTTTCTAATCCTCTTCCTCCTCTTTGTGCTCGCCACCTTCGCCGTCGGAATCTTCGCCGTCGTCCACCGCAACCACAATTACACCGTCCTCTCCTCCTTCTCCTACGACTCGGACTCCGCCTCCTGCGTCAAAAACGAAACTTTGACCCGTCTCCCCAACTGGGTCGGACCCACTTTCTCCTCTTCCTCCTCTCTGCTCAAAGATTTGACATGGACCCTCGTGATTACGTTGATTCTAAGCCTGCCCATTTGCTTCAGTCTGCTTATTCTCCTCAAACACTACGCCAAGCAAGTCGTCTACGTCTTCCTCCCCTTCTTCATCGTCTTCCCAATCTTCTTCAACGTCTACTGGTTCGTCGCCTGCACTCTCAGCTCCACCTGCAGTGACGCTCTCCCTTTAGTTTACAGAATTTTGATTCTGATCTTCGTGTTTTTGGTAATTGGGGTCATTGTGTGGATTCTTGTGGTGAATTGGCATCGGATTGAGCTCACTGTGAGCATAATCGGGGTGGCATCCCATGCGCTTTCACGGAACTTGGGCTTGTTCGGGGTTCTGCCGTGTTTGACATTAGGATTGGTGGTTTACTATGCGCCCATTGTTGTGTTCTTGGTCTATGCCAGGTTCAATGGGAAGATTGCGCCGCGGGAATCGAATGGAGTGTATAGTTGTGTGTGGAAGCAGGATGGTTGGGTGCCTGCTTATTACGCATTGGCGATTTTGACAATGTTGTGGTCGTTTACGACGATGGTGGAAGCTCAGGTGTATGTGATTAGTGGGACTGTTGCTCAGTGGTATTTCACTAAGGAGGATTCAACTCCGAAGCGGATTATCAGGAGCTCTTTAAGAAATGCTTTTGGCCCCAACTTTGGCACAGTATGCCTATCTGGATTACTTTTATCTGTTGTTCGAGTTGTTCGTGCCATGGTCGATGGTGCAAGACAAGACGGTTCACCTGGGATGATGTATTTTTTAATGCGCTGCTGTGTTAATGCCTTATTTTCAGCGATTGATTTTCTCAACAAGTTCACTATCAACTTTGCGGCAATAACTGGTGAAGCTTACTGCTCATCTGCAAAAATGACATATGAGCTTCTAAAGCGTAATCTTCTCTCTGCGGTTTTTGTGGAAACCATCTCCACTCGTCTATTGGCTGGAATTGTTTTTGTACTCTCAGCGATATATGCAATCGTGGTCTGCGCTATCTTACAAGCTGCGACCAGTCTTGGGGTTGATTCGTACTTTGTGGCAGTTCTGGCGTGGGTGCTACTGATCGTGGTACTGTGTTTCTTTGTTCATGTGCTGGACAATGTAGTTGACACCGTGTATGTGTGCTACGCCATAGATAGGGATAGAGGAGAGGTTTGTAAACAGGAAGTTCACGAGGTTTACATTCACTTACCCATTAGTCGAAACCATAGATCATCTTCTATGATCTCCAGAACTCTGGGTGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Choline_transpo

Protein Analysis

555

Amino Acids

61.73

Weight (kDa)

8.12

Isoelectric Point (pI)

44.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Choline_transpo PF04515 211 - 520 1.4e-67 Plasma-membrane choline transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 596
Acc36I ACCTGC 1 cut(s) 596
AccB1I GGYRCC 1 cut(s) 901
AccI GTMKAC 2 cut(s) 507, 555
AccII CGCG 1 cut(s) 853
AcsI RAATTY 1 cut(s) 618
AcuI CTGAAG 1 cut(s) 448
AfaI GTAC 4 cut(s) 161, 1377, 1448, 1488
AfiI CCNNNNNNNGG 4 cut(s) 370, 1076, 1434, 1554
AgsI TTSAA 4 cut(s) 199, 550, 835, 1023
AjnI CCWGG 2 cut(s) 827, 1162
AluBI AGCT 8 cut(s) 9, 582, 697, 965, 1047, 1268, 1300, 1421
AluI AGCT 8 cut(s) 9, 582, 697, 965, 1047, 1268, 1300, 1421
Alw21I GWGCWC 3 cut(s) 240, 699, 1049
Alw26I GTCTC 1 cut(s) 365
AoxI GGCC 2 cut(s) 184, 1066
ApeKI GCWGC 3 cut(s) 6, 1188, 1421
ApoI RAATTY 1 cut(s) 618
AspLEI GCGC 5 cut(s) 733, 802, 850, 1188, 1409
AspS9I GGNCC 4 cut(s) 185, 377, 423, 1067
AsuHPI GGTGA 3 cut(s) 688, 1152, 1274
AvaII GGWCC 2 cut(s) 377, 423
BanI GGYRCC 1 cut(s) 901
BanII GRGCYC 2 cut(s) 699, 1049
BauI CACGAG 2 cut(s) 428, 1589
BbsI GAAGAC 2 cut(s) 505, 526
Bbv12I GWGCWC 3 cut(s) 240, 699, 1049
BbvI GCAGC 3 cut(s) 18, 1175, 1408
BccI CCATC 6 cut(s) 38, 124, 887, 949, 1133, 1349
BceAI ACGGC 4 cut(s) 199, 236, 251, 751
BciT130I CCWGG 2 cut(s) 829, 1164
BcoDI GTCTC 1 cut(s) 365
BfmI CTRYAG 1 cut(s) 589
BfuAI ACCTGC 1 cut(s) 596
BglII AGATCT 1 cut(s) 168
BisI GCNGC 5 cut(s) 7, 851, 1189, 1251, 1422
BlsI GCNGC 5 cut(s) 8, 852, 1190, 1252, 1423
Bme1390I CCNGG 2 cut(s) 829, 1164
Bme18I GGWCC 2 cut(s) 377, 423
BmgT120I GGNCC 4 cut(s) 185, 377, 423, 1067
BmiI GGNNCC 5 cut(s) 187, 379, 425, 903, 1069
BmrFI CCNGG 2 cut(s) 829, 1164
BmrI ACTGGG 2 cut(s) 151, 379
BmsI GCATC 3 cut(s) 133, 694, 731
BmuI ACTGGG 2 cut(s) 151, 379
BpiI GAAGAC 2 cut(s) 505, 526
BpmI CTGGAG 2 cut(s) 149, 1630
Bpu10I CCTNAGC 1 cut(s) 966
BsaBI GATNNNNATC 1 cut(s) 629
BsaJI CCNNGG 4 cut(s) 181, 851, 1131, 1163
BsaWI WCCGGW 1 cut(s) 133
BsaXI ACNNNNNCTCC 4 cut(s) 308, 314, 338, 344
Bsc4I CCNNNNNNNGG 4 cut(s) 370, 1076, 1434, 1554
Bse1I ACTGG 5 cut(s) 157, 374, 563, 1264, 1428
Bse8I GATNNNNATC 1 cut(s) 629
BseBI CCWGG 2 cut(s) 829, 1164
BseDI CCNNGG 4 cut(s) 181, 851, 1131, 1163
BseGI GGATG 4 cut(s) 44, 722, 898, 1173
BseJI GATNNNNATC 1 cut(s) 629
BseLI CCNNNNNNNGG 4 cut(s) 370, 1076, 1434, 1554
BseMII CTCAG 4 cut(s) 592, 980, 1010, 1395
BseNI ACTGG 5 cut(s) 157, 374, 563, 1264, 1428
BseRI GAGGAG 7 cut(s) 56, 218, 292, 379, 388, 470, 1578
BseXI GCAGC 3 cut(s) 18, 1175, 1408
BsgI GTGCAG 1 cut(s) 556
Bsh1236I CGCG 1 cut(s) 853
BshFI GGCC 2 cut(s) 186, 1068
BshNI GGYRCC 1 cut(s) 901
BsiHKAI GWGCWC 3 cut(s) 240, 699, 1049
BsiSI CCGG 1 cut(s) 134
BslFI GGGAC 2 cut(s) 91, 1000
BslI CCNNNNNNNGG 4 cut(s) 370, 1076, 1434, 1554
BsmAI GTCTC 1 cut(s) 365
BsmBI CGTCTC 1 cut(s) 365
BsmFI GGGAC 2 cut(s) 91, 1000
BsnI GGCC 2 cut(s) 186, 1068
Bsp1286I GDGCHC 3 cut(s) 240, 699, 1049
Bsp143I GATC 5 cut(s) 168, 630, 1479, 1628, 1641
Bsp19I CCATGG 1 cut(s) 1131
BspANI GGCC 2 cut(s) 186, 1068
BspCNI CTCAG 4 cut(s) 591, 979, 1009, 1394
BspFNI CGCG 1 cut(s) 853
BspLI GGNNCC 5 cut(s) 187, 379, 425, 903, 1069
BspMAI CTGCAG 1 cut(s) 593
BspMI ACCTGC 1 cut(s) 596
BspT107I GGYRCC 1 cut(s) 901
BsrI ACTGG 5 cut(s) 157, 374, 563, 1264, 1428
BssECI CCNNGG 4 cut(s) 181, 851, 1131, 1163
BssMI GATC 5 cut(s) 168, 630, 1479, 1628, 1641
BssSI CACGAG 2 cut(s) 428, 1589
BssT1I CCWWGG 1 cut(s) 1131
Bst2BI CACGAG 2 cut(s) 428, 1589
Bst2UI CCWGG 2 cut(s) 829, 1164
Bst4CI ACNGT 8 cut(s) 105, 295, 703, 991, 1084, 1157, 1491, 1531
Bst6I CTCTTC 2 cut(s) 227, 397
BstAPI GCANNNNNTGC 2 cut(s) 728, 1086
BstC8I GCNNGC 4 cut(s) 240, 452, 571, 906
BstDEI CTNAG 6 cut(s) 446, 578, 966, 996, 1011, 1381
BstDSI CCRYGG 3 cut(s) 181, 851, 1131
BstF5I GGATG 4 cut(s) 44, 722, 898, 1173
BstFNI CGCG 1 cut(s) 853
BstHHI GCGC 5 cut(s) 733, 802, 850, 1188, 1409
BstKTI GATC 5 cut(s) 171, 633, 1482, 1631, 1644
BstMAI GTCTC 1 cut(s) 365
BstMBI GATC 5 cut(s) 168, 630, 1479, 1628, 1641
BstMWI GCNNNNNNNGC 9 cut(s) 248, 579, 588, 728, 914, 1086, 1306, 1391, 1463
BstNI CCWGG 2 cut(s) 829, 1164
BstSCI CCNGG 2 cut(s) 827, 1162
BstSFI CTRYAG 1 cut(s) 589
BstUI CGCG 1 cut(s) 853
BstV1I GCAGC 3 cut(s) 18, 1175, 1408
BstV2I GAAGAC 2 cut(s) 505, 526
BstX2I RGATCY 1 cut(s) 168
BstXI CCANNNNNNTGG 1 cut(s) 1654
BstYI RGATCY 1 cut(s) 168
BsuRI GGCC 2 cut(s) 186, 1068
BtgI CCRYGG 3 cut(s) 181, 851, 1131
BtsCI GGATG 4 cut(s) 44, 722, 898, 1173
BtsI GCAGTG 1 cut(s) 598
BtsIMutI CAGTG 3 cut(s) 598, 699, 1004
BveI ACCTGC 1 cut(s) 596
Cac8I GCNNGC 4 cut(s) 240, 452, 571, 906
CfoI GCGC 5 cut(s) 733, 802, 850, 1188, 1409
Cfr13I GGNCC 4 cut(s) 185, 377, 423, 1067
Cfr42I CCGCGG 1 cut(s) 854
CseI GACGC 2 cut(s) 325, 605
Csp6I GTAC 4 cut(s) 160, 1376, 1447, 1487
CviAII CATG 4 cut(s) 420, 728, 1132, 1505
CviQI GTAC 4 cut(s) 160, 1376, 1447, 1487
DdeI CTNAG 6 cut(s) 446, 578, 966, 996, 1011, 1381
DpnI GATC 5 cut(s) 170, 632, 1481, 1630, 1643
DpnII GATC 5 cut(s) 168, 630, 1479, 1628, 1641
Eam1104I CTCTTC 2 cut(s) 227, 397
EarI CTCTTC 2 cut(s) 227, 397
EciI GGCGGA 2 cut(s) 102, 316
Ecl136II GAGCTC 2 cut(s) 697, 1047
Eco130I CCWWGG 1 cut(s) 1131
Eco24I GRGCYC 2 cut(s) 699, 1049
Eco47I GGWCC 2 cut(s) 377, 423
Eco53kI GAGCTC 2 cut(s) 697, 1047
Eco57I CTGAAG 1 cut(s) 448
EcoICRI GAGCTC 2 cut(s) 697, 1047
EcoRII CCWGG 2 cut(s) 827, 1162
EcoT14I CCWWGG 1 cut(s) 1131
EcoT38I GRGCYC 2 cut(s) 699, 1049
ErhI CCWWGG 1 cut(s) 1131
Esp3I CGTCTC 1 cut(s) 365
FaeI CATG 4 cut(s) 423, 731, 1135, 1508
FalI AAGNNNNNCTT 4 cut(s) 17, 49, 1045, 1077
FaqI GGGAC 2 cut(s) 91, 1000
FatI CATG 4 cut(s) 419, 727, 1131, 1504
FauI CCCGC 2 cut(s) 197, 846
FauNDI CATATG 1 cut(s) 1294
FblI GTMKAC 2 cut(s) 507, 555
Fnu4HI GCNGC 5 cut(s) 7, 851, 1189, 1251, 1422
FokI GGATG 4 cut(s) 31, 709, 905, 1180
FriOI GRGCYC 2 cut(s) 699, 1049
Fsp4HI GCNGC 5 cut(s) 7, 851, 1189, 1251, 1422
GlaI GCGC 5 cut(s) 732, 801, 849, 1187, 1408
GluI GCNGC 5 cut(s) 7, 851, 1189, 1251, 1422
GsuI CTGGAG 2 cut(s) 149, 1630
HaeIII GGCC 2 cut(s) 186, 1068
HapII CCGG 1 cut(s) 134
HgaI GACGC 2 cut(s) 325, 605
HhaI GCGC 5 cut(s) 733, 802, 850, 1188, 1409
Hin1II CATG 4 cut(s) 423, 731, 1135, 1508
Hin6I GCGC 5 cut(s) 731, 800, 848, 1186, 1407
HinP1I GCGC 5 cut(s) 731, 800, 848, 1186, 1407
HincII GTYRAC 1 cut(s) 1525
HindII GTYRAC 1 cut(s) 1525
HindIII AAGCTT 1 cut(s) 1266
HpaII CCGG 1 cut(s) 134
HphI GGTGA 3 cut(s) 688, 1152, 1274
Hpy188I TCNGA 8 cut(s) 13, 142, 257, 322, 377, 630, 690, 1029
Hpy188III TCNNGA 6 cut(s) 166, 430, 1042, 1095, 1589, 1647
Hpy99I CGWCG 4 cut(s) 257, 272, 569, 955
HpyAV CCTTC 3 cut(s) 256, 316, 532
HpyCH4III ACNGT 8 cut(s) 105, 295, 703, 991, 1084, 1157, 1491, 1531
HpyCH4IV ACGT 3 cut(s) 437, 510, 552
HpyCH4V TGCA 5 cut(s) 573, 591, 1145, 1283, 1394
HpyF10VI GCNNNNNNNGC 9 cut(s) 248, 579, 588, 728, 914, 1086, 1306, 1391, 1463
HpyF3I CTNAG 6 cut(s) 446, 578, 966, 996, 1011, 1381
HpySE526I ACGT 3 cut(s) 437, 510, 552
Hsp92II CATG 4 cut(s) 423, 731, 1135, 1508
HspAI GCGC 5 cut(s) 731, 800, 848, 1186, 1407
KspI CCGCGG 1 cut(s) 854
Kzo9I GATC 5 cut(s) 168, 630, 1479, 1628, 1641
LmnI GCTCC 3 cut(s) 14, 587, 1044
Lsp1109I GCAGC 3 cut(s) 18, 1175, 1408
LweI GCATC 3 cut(s) 133, 694, 731
MaeII ACGT 3 cut(s) 437, 510, 552
MaeIII GTNAC 1 cut(s) 593
MalI GATC 5 cut(s) 170, 632, 1481, 1630, 1643
MboI GATC 5 cut(s) 168, 630, 1479, 1628, 1641
MflI RGATCY 1 cut(s) 168
MhlI GDGCHC 3 cut(s) 240, 699, 1049
MluCI AATT 5 cut(s) 286, 618, 648, 679, 1365
MlyI GAGTC 3 cut(s) 35, 311, 317
MmeI TCCRAC 2 cut(s) 235, 355
MseI TTAA 3 cut(s) 1052, 1181, 1197
MslI CAYNNNNRTG 1 cut(s) 809
MspA1I CMGCKG 1 cut(s) 853
MspI CCGG 1 cut(s) 134
MspR9I CCNGG 2 cut(s) 829, 1164
MvaI CCWGG 2 cut(s) 829, 1164
MvnI CGCG 1 cut(s) 853
MwoI GCNNNNNNNGC 9 cut(s) 248, 579, 588, 728, 914, 1086, 1306, 1391, 1463
NcoI CCATGG 1 cut(s) 1131
NdeI CATATG 1 cut(s) 1294
NdeII GATC 5 cut(s) 168, 630, 1479, 1628, 1641
NlaIII CATG 4 cut(s) 423, 731, 1135, 1508
NlaIV GGNNCC 5 cut(s) 187, 379, 425, 903, 1069
NmuCI GTSAC 1 cut(s) 593
PaqCI CACCTGC 1 cut(s) 596
PcsI WCGNNNNNNNCGW 2 cut(s) 950, 1123
PfeI GAWTC 8 cut(s) 137, 258, 442, 625, 667, 857, 1019, 1442
PkrI GCNGC 5 cut(s) 8, 852, 1190, 1252, 1423
PleI GAGTC 3 cut(s) 35, 311, 317
PpsI GAGTC 3 cut(s) 35, 311, 317
Psp124BI GAGCTC 2 cut(s) 699, 1049
Psp6I CCWGG 2 cut(s) 827, 1162
PspGI CCWGG 2 cut(s) 827, 1162
PspN4I GGNNCC 5 cut(s) 187, 379, 425, 903, 1069
PspPI GGNCC 4 cut(s) 185, 377, 423, 1067
PstI CTGCAG 1 cut(s) 593
PsuI RGATCY 1 cut(s) 168
RsaI GTAC 4 cut(s) 161, 1377, 1448, 1488
RsaNI GTAC 4 cut(s) 160, 1376, 1447, 1487
RseI CAYNNNNRTG 1 cut(s) 809
SacI GAGCTC 2 cut(s) 699, 1049
SacII CCGCGG 1 cut(s) 854
SaqAI TTAA 3 cut(s) 1052, 1181, 1197
SatI GCNGC 5 cut(s) 7, 851, 1189, 1251, 1422
Sau3AI GATC 5 cut(s) 168, 630, 1479, 1628, 1641
Sau96I GGNCC 4 cut(s) 185, 377, 423, 1067
SchI GAGTC 3 cut(s) 35, 311, 317
ScrFI CCNGG 2 cut(s) 829, 1164
SduI GDGCHC 3 cut(s) 240, 699, 1049
SfaNI GCATC 3 cut(s) 133, 694, 731
SfcI CTRYAG 1 cut(s) 589
Sfr303I CCGCGG 1 cut(s) 854
SgrBI CCGCGG 1 cut(s) 854
SinI GGWCC 2 cut(s) 377, 423
SmiMI CAYNNNNRTG 1 cut(s) 809
Sse9I AATT 5 cut(s) 286, 618, 648, 679, 1365
SstI GAGCTC 2 cut(s) 699, 1049
StyD4I CCNGG 2 cut(s) 827, 1162
StyI CCWWGG 1 cut(s) 1131
TaaI ACNGT 8 cut(s) 105, 295, 703, 991, 1084, 1157, 1491, 1531
TaiI ACGT 3 cut(s) 440, 513, 555
TaqI TCGA 5 cut(s) 84, 860, 1117, 1137, 1618
TasI AATT 5 cut(s) 286, 618, 648, 679, 1365
TatI WGTACW 1 cut(s) 1375
TauI GCSGC 2 cut(s) 853, 1253
TfiI GAWTC 8 cut(s) 137, 258, 442, 625, 667, 857, 1019, 1442
Tru1I TTAA 3 cut(s) 1052, 1181, 1197
Tru9I TTAA 3 cut(s) 1052, 1181, 1197
TscAI CASTG 3 cut(s) 598, 706, 1004
TseFI GTSAC 1 cut(s) 593
TseI GCWGC 3 cut(s) 6, 1188, 1421
Tsp45I GTSAC 1 cut(s) 593
TspDTI ATGAA 2 cut(s) 517, 1493
TspGWI ACGGA 1 cut(s) 754
TspRI CASTG 3 cut(s) 598, 706, 1004
VpaK11BI GGWCC 2 cut(s) 377, 423
XapI RAATTY 1 cut(s) 618
XcmI CCANNNNNNNNNTGG 1 cut(s) 1354
XmiI GTMKAC 2 cut(s) 507, 555
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.