MD05G1128500.v1.1

Coiled-coil domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0008369
Physical Location & Seq
Forward (+)
25241027 .. 25243920
2894 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1128500.v1.1.491

Sequence Viewer

Length: 690 bp
ATGCCGAAAAAGATGGGTTTGAATAGCAAGGCGGAGGCCGCCAAGGCTCGGAAGAGCTCCACCGAAGCCGAGCGCAAGGAGCGCGAGGCTCGCGAGAAGGAGGAGCAGTACTGGCGCGAGGCCGAGGGCAGCAAGTCCCGCGCGGCAAAGAAGCGCGAGGAAGAAGCCGAGAAGCGAGCGGAGGCCGCCGCGCGGAAAGCCGAGGCGCGCCGATTGGCGGAGCTGGAGGAGAAGGAACTCGAGAAGGCGGGGAAGAAGCCCGATAAGAAGGTCGGGCGAGTCACGATCCCGGTTCCAAAGGTGACCGAGGCCGAGCTGAGACGGCGGAAAGAGGAGGAGCAGGCGGCGTTGGAGAAGAAATCGGATGAGGTGAAGAAGAAGCAGAGCCGTACGGCAGAGGAGGAGGAGTATGAGAGGATGGTGGTGGTGGAGAATAAGAATCGGGATGATTCGATTATTGAGGCGAGGACGGTGGAGGAGGCGGTTGCGCAGATGTCGGTGGCGGAGAGCTTGCCGGTGGATCGCCACCCGGAAAGGAGGCTCAAGGCTTCGTTTAAGGCTTTTGAAGAAGCTGAGCTCCCCAGGCTGAAGGAAGAGAAACCAGGTCTTACGCACAATCAATACAAGGACATGATATGGAAGCTGTGGAAGAAATCTCCGGATAACCCTCTAAATCAGCTTGCTGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ccdc124

Protein Analysis

230

Amino Acids

26.58

Weight (kDa)

9.08

Isoelectric Point (pI)

59.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ccdc124 PF06244 147 - 226 7e-34 Coiled-coil domain-containing protein 124 /Oxs1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 489
AccBSI CCGCTC 1 cut(s) 179
AccII CGCG 9 cut(s) 84, 93, 117, 141, 143, 156, 191, 193, 208
AccIII TCCGGA 1 cut(s) 658
AclWI GGATC 2 cut(s) 280, 528
AcuI CTGAAG 1 cut(s) 608
AfaI GTAC 2 cut(s) 110, 391
AfiI CCNNNNNNNGG 3 cut(s) 48, 192, 217
AgsI TTSAA 2 cut(s) 22, 566
AjnI CCWGG 2 cut(s) 581, 601
AluBI AGCT 8 cut(s) 57, 223, 316, 510, 572, 577, 643, 679
AluI AGCT 8 cut(s) 57, 223, 316, 510, 572, 577, 643, 679
Alw21I GWGCWC 2 cut(s) 59, 579
Alw26I GTCTC 1 cut(s) 313
AlwI GGATC 2 cut(s) 280, 528
Ama87I CYCGRG 1 cut(s) 239
Aor13HI TCCGGA 1 cut(s) 658
AoxI GGCC 4 cut(s) 36, 120, 183, 309
ApeKI GCWGC 1 cut(s) 129
AscI GGCGCGCC 1 cut(s) 206
AspLEI GCGC 9 cut(s) 75, 84, 117, 143, 156, 193, 208, 210, 490
AsuC2I CCSGG 2 cut(s) 290, 530
AsuHPI GGTGA 2 cut(s) 313, 382
AvaI CYCGRG 1 cut(s) 239
BanII GRGCYC 2 cut(s) 59, 579
Bbv12I GWGCWC 2 cut(s) 59, 579
BbvI GCAGC 1 cut(s) 141
BccI CCATC 2 cut(s) 7, 412
BceAI ACGGC 3 cut(s) 338, 372, 408
BciT130I CCWGG 2 cut(s) 583, 603
BcnI CCSGG 2 cut(s) 290, 530
BcoDI GTCTC 1 cut(s) 313
BglI GCCNNNNNGGC 1 cut(s) 44
BisI GCNGC 6 cut(s) 39, 130, 144, 186, 189, 345
BlpI GCTNAGC 1 cut(s) 573
BlsI GCNGC 6 cut(s) 40, 131, 145, 187, 190, 346
BmcAI AGTACT 1 cut(s) 110
Bme1390I CCNGG 4 cut(s) 290, 530, 583, 603
BmeT110I CYCGRG 1 cut(s) 239
BmiI GGNNCC 1 cut(s) 294
BmrFI CCNGG 4 cut(s) 290, 530, 583, 603
BpmI CTGGAG 1 cut(s) 245
Bpu1102I GCTNAGC 1 cut(s) 573
BpuEI CTTGAG 1 cut(s) 527
BpuMI CCSGG 2 cut(s) 290, 530
BsaJI CCNNGG 5 cut(s) 42, 123, 201, 306, 581
BsaWI WCCGGW 1 cut(s) 658
BsaXI ACNNNNNCTCC 4 cut(s) 92, 122, 392, 422
Bsc4I CCNNNNNNNGG 3 cut(s) 48, 192, 217
Bse118I RCCGGY 1 cut(s) 514
Bse1I ACTGG 1 cut(s) 116
BseAI TCCGGA 1 cut(s) 658
BseBI CCWGG 2 cut(s) 583, 603
BseDI CCNNGG 5 cut(s) 42, 123, 201, 306, 581
BseGI GGATG 3 cut(s) 370, 423, 451
BseLI CCNNNNNNNGG 3 cut(s) 48, 192, 217
BseMII CTCAG 3 cut(s) 308, 564, 675
BseNI ACTGG 1 cut(s) 116
BsePI GCGCGC 1 cut(s) 206
BseRI GAGGAG 8 cut(s) 116, 242, 347, 350, 413, 416, 419, 491
BseXI GCAGC 1 cut(s) 141
Bsh1236I CGCG 9 cut(s) 84, 93, 117, 141, 143, 156, 191, 193, 208
BshFI GGCC 4 cut(s) 38, 122, 185, 311
BsiHKAI GWGCWC 2 cut(s) 59, 579
BsiHKCI CYCGRG 1 cut(s) 239
BsiSI CCGG 4 cut(s) 290, 515, 530, 659
BsiWI CGTACG 1 cut(s) 389
BslFI GGGAC 1 cut(s) 121
BslI CCNNNNNNNGG 3 cut(s) 48, 192, 217
BsmAI GTCTC 1 cut(s) 313
BsmBI CGTCTC 1 cut(s) 313
BsmFI GGGAC 1 cut(s) 121
BsnI GGCC 4 cut(s) 38, 122, 185, 311
BsoBI CYCGRG 1 cut(s) 239
Bsp1286I GDGCHC 2 cut(s) 59, 579
Bsp13I TCCGGA 1 cut(s) 658
Bsp143I GATC 2 cut(s) 285, 520
Bsp1720I GCTNAGC 1 cut(s) 573
Bsp68I TCGCGA 1 cut(s) 93
BspANI GGCC 4 cut(s) 38, 122, 185, 311
BspCNI CTCAG 3 cut(s) 309, 565, 676
BspEI TCCGGA 1 cut(s) 658
BspFNI CGCG 9 cut(s) 84, 93, 117, 141, 143, 156, 191, 193, 208
BspLI GGNNCC 1 cut(s) 294
BspPI GGATC 2 cut(s) 280, 528
BspQI GCTCTTC 1 cut(s) 47
BsrBI CCGCTC 1 cut(s) 179
BsrFI RCCGGY 1 cut(s) 514
BsrI ACTGG 1 cut(s) 116
BssAI RCCGGY 1 cut(s) 514
BssECI CCNNGG 5 cut(s) 42, 123, 201, 306, 581
BssHII GCGCGC 1 cut(s) 206
BssMI GATC 2 cut(s) 285, 520
BssT1I CCWWGG 1 cut(s) 42
Bst2UI CCWGG 2 cut(s) 583, 603
Bst4CI ACNGT 1 cut(s) 472
Bst6I CTCTTC 2 cut(s) 47, 588
BstC8I GCNNGC 6 cut(s) 91, 177, 208, 342, 512, 681
BstDEI CTNAG 3 cut(s) 317, 573, 684
BstEII GGTNACC 1 cut(s) 301
BstF5I GGATG 3 cut(s) 370, 423, 451
BstFNI CGCG 9 cut(s) 84, 93, 117, 141, 143, 156, 191, 193, 208
BstHHI GCGC 9 cut(s) 75, 84, 117, 143, 156, 193, 208, 210, 490
BstKTI GATC 2 cut(s) 288, 523
BstMAI GTCTC 1 cut(s) 313
BstMBI GATC 2 cut(s) 285, 520
BstNI CCWGG 2 cut(s) 583, 603
BstPI GGTNACC 1 cut(s) 301
BstSCI CCNGG 4 cut(s) 288, 528, 581, 601
BstUI CGCG 9 cut(s) 84, 93, 117, 141, 143, 156, 191, 193, 208
BstV1I GCAGC 1 cut(s) 141
BsuRI GGCC 4 cut(s) 38, 122, 185, 311
BtsCI GGATG 3 cut(s) 370, 423, 451
BtuMI TCGCGA 1 cut(s) 93
Cac8I GCNNGC 6 cut(s) 91, 177, 208, 342, 512, 681
CfoI GCGC 9 cut(s) 75, 84, 117, 143, 156, 193, 208, 210, 490
Cfr10I RCCGGY 1 cut(s) 514
CsiI ACCWGGT 1 cut(s) 601
Csp6I GTAC 2 cut(s) 109, 390
CviAII CATG 1 cut(s) 631
CviQI GTAC 2 cut(s) 109, 390
DdeI CTNAG 3 cut(s) 317, 573, 684
DpnI GATC 2 cut(s) 287, 522
DpnII GATC 2 cut(s) 285, 520
Eam1104I CTCTTC 2 cut(s) 47, 588
EarI CTCTTC 2 cut(s) 47, 588
EciI GGCGGA 4 cut(s) 47, 233, 340, 518
Ecl136II GAGCTC 2 cut(s) 57, 577
Eco130I CCWWGG 1 cut(s) 42
Eco24I GRGCYC 2 cut(s) 59, 579
Eco53kI GAGCTC 2 cut(s) 57, 577
Eco57I CTGAAG 1 cut(s) 608
Eco88I CYCGRG 1 cut(s) 239
Eco91I GGTNACC 1 cut(s) 301
EcoICRI GAGCTC 2 cut(s) 57, 577
EcoO65I GGTNACC 1 cut(s) 301
EcoRII CCWGG 2 cut(s) 581, 601
EcoT14I CCWWGG 1 cut(s) 42
EcoT38I GRGCYC 2 cut(s) 59, 579
ErhI CCWWGG 1 cut(s) 42
Esp3I CGTCTC 1 cut(s) 313
FaeI CATG 1 cut(s) 634
FaiI YATR 3 cut(s) 411, 632, 637
FaqI GGGAC 1 cut(s) 121
FatI CATG 1 cut(s) 630
FauI CCCGC 2 cut(s) 146, 241
Fnu4HI GCNGC 6 cut(s) 39, 130, 144, 186, 189, 345
FokI GGATG 3 cut(s) 377, 430, 458
FriOI GRGCYC 2 cut(s) 59, 579
Fsp4HI GCNGC 6 cut(s) 39, 130, 144, 186, 189, 345
FspI TGCGCA 1 cut(s) 489
GlaI GCGC 9 cut(s) 74, 83, 116, 142, 155, 192, 207, 209, 489
GluI GCNGC 6 cut(s) 39, 130, 144, 186, 189, 345
GsuI CTGGAG 1 cut(s) 245
HaeIII GGCC 4 cut(s) 38, 122, 185, 311
HapII CCGG 4 cut(s) 290, 515, 530, 659
HhaI GCGC 9 cut(s) 75, 84, 117, 143, 156, 193, 208, 210, 490
Hin1II CATG 1 cut(s) 634
Hin6I GCGC 9 cut(s) 73, 82, 115, 141, 154, 191, 206, 208, 488
HinP1I GCGC 9 cut(s) 73, 82, 115, 141, 154, 191, 206, 208, 488
HinfI GANTC 3 cut(s) 279, 439, 449
HpaII CCGG 4 cut(s) 290, 515, 530, 659
HphI GGTGA 2 cut(s) 313, 382
Hpy188I TCNGA 2 cut(s) 51, 364
Hpy188III TCNNGA 5 cut(s) 92, 241, 283, 443, 659
HpyAV CCTTC 5 cut(s) 91, 226, 238, 262, 583
HpyCH4III ACNGT 1 cut(s) 472
HpyF3I CTNAG 3 cut(s) 317, 573, 684
Hsp92II CATG 1 cut(s) 634
HspAI GCGC 9 cut(s) 73, 82, 115, 141, 154, 191, 206, 208, 488
Kpn2I TCCGGA 1 cut(s) 658
Kzo9I GATC 2 cut(s) 285, 520
LguI GCTCTTC 1 cut(s) 47
LmnI GCTCC 6 cut(s) 62, 79, 103, 220, 337, 582
Lsp1109I GCAGC 1 cut(s) 141
MabI ACCWGGT 1 cut(s) 601
MaeIII GTNAC 2 cut(s) 280, 301
MalI GATC 2 cut(s) 287, 522
MbiI CCGCTC 1 cut(s) 179
MboI GATC 2 cut(s) 285, 520
MboII GAAGA 9 cut(s) 64, 173, 265, 367, 385, 388, 578, 605, 661
MhlI GDGCHC 2 cut(s) 59, 579
MlyI GAGTC 1 cut(s) 288
MmeI TCCRAC 1 cut(s) 330
MroI TCCGGA 1 cut(s) 658
MseI TTAA 1 cut(s) 555
MspI CCGG 4 cut(s) 290, 515, 530, 659
MspR9I CCNGG 4 cut(s) 290, 530, 583, 603
MvaI CCWGG 2 cut(s) 583, 603
MvnI CGCG 9 cut(s) 84, 93, 117, 141, 143, 156, 191, 193, 208
NciI CCSGG 2 cut(s) 290, 530
NdeII GATC 2 cut(s) 285, 520
NlaIII CATG 1 cut(s) 634
NlaIV GGNNCC 1 cut(s) 294
NmeAIII GCCGAG 5 cut(s) 94, 148, 193, 226, 337
NmuCI GTSAC 2 cut(s) 280, 301
NruI TCGCGA 1 cut(s) 93
NsbI TGCGCA 1 cut(s) 489
PaeR7I CTCGAG 1 cut(s) 239
PalAI GGCGCGCC 1 cut(s) 206
PauI GCGCGC 1 cut(s) 206
PciSI GCTCTTC 1 cut(s) 47
PfeI GAWTC 2 cut(s) 439, 449
Pfl23II CGTACG 1 cut(s) 389
PkrI GCNGC 6 cut(s) 40, 131, 145, 187, 190, 346
PleI GAGTC 1 cut(s) 287
PpsI GAGTC 1 cut(s) 287
Psp124BI GAGCTC 2 cut(s) 59, 579
Psp6I CCWGG 2 cut(s) 581, 601
PspEI GGTNACC 1 cut(s) 301
PspGI CCWGG 2 cut(s) 581, 601
PspLI CGTACG 1 cut(s) 389
PspN4I GGNNCC 1 cut(s) 294
PteI GCGCGC 1 cut(s) 206
RruI TCGCGA 1 cut(s) 93
RsaI GTAC 2 cut(s) 110, 391
RsaNI GTAC 2 cut(s) 109, 390
SacI GAGCTC 2 cut(s) 59, 579
SapI GCTCTTC 1 cut(s) 47
SaqAI TTAA 1 cut(s) 555
SatI GCNGC 6 cut(s) 39, 130, 144, 186, 189, 345
Sau3AI GATC 2 cut(s) 285, 520
ScaI AGTACT 1 cut(s) 110
SchI GAGTC 1 cut(s) 288
ScrFI CCNGG 4 cut(s) 290, 530, 583, 603
SduI GDGCHC 2 cut(s) 59, 579
SexAI ACCWGGT 1 cut(s) 601
Sfr274I CTCGAG 1 cut(s) 239
SgsI GGCGCGCC 1 cut(s) 206
SlaI CTCGAG 1 cut(s) 239
SmlI CTYRAG 2 cut(s) 239, 542
SmoI CTYRAG 2 cut(s) 239, 542
SstI GAGCTC 2 cut(s) 59, 579
StyD4I CCNGG 4 cut(s) 288, 528, 581, 601
StyI CCWWGG 1 cut(s) 42
TaaI ACNGT 1 cut(s) 472
TaqI TCGA 2 cut(s) 240, 452
TaqII GACCGA 1 cut(s) 320
TatI WGTACW 1 cut(s) 108
TauI GCSGC 5 cut(s) 41, 146, 188, 191, 347
TfiI GAWTC 2 cut(s) 439, 449
Tru1I TTAA 1 cut(s) 555
Tru9I TTAA 1 cut(s) 555
TseFI GTSAC 2 cut(s) 280, 301
TseI GCWGC 1 cut(s) 129
Tsp45I GTSAC 2 cut(s) 280, 301
XhoI CTCGAG 1 cut(s) 239
ZrmI AGTACT 1 cut(s) 110
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.