Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0012525

Physical Location & Seq

Genomic Coordinates Reverse (-)

28265893 .. 28269051

Gene Structure 3159 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1152000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 822 bp

ATGGCTATTCTCTCTGCTCAATTCGCCTCTGCAGTAGCACTGATTGCGGTGGGCGCTTCTCTGTGCCTGATCGATGCCAAGCAAGCCGGAATCGGATTCGAAATCGGAAGCAGAGGAGGAGGAGGGCAGAGGGAGTTCGATTACTTCAACTTGGCCCTGCAATGGCCTGGCACTTTCTGTCAGCGCACCCGCCATTGTTGCTCCTCCAATGCTTGCTGCCGCGGCTCAAATGCTCCAACCATGTTTACAATCCATGGATTGTGGCCTGACTACAATGATGGAACTTGGCCTGCCTGTTGCACACGGAAAACCTTTGATGATAAGGAGATCTCAACATTGCACGATGCTTTAGAGAAATACTGGCCATCTTTAAGCTGTGGTAAACCATCATCCTGCCATGGTGGAAAAGGATCATTTTGGGGTCATGAGGTGAAGCACGGAACTTGCTCCTCTCCAGTAGTTGGAGATGAATACAATTACTTTTTGACAACCCTCAATGTCTATTTTAAGTACAATGTCACTCAAATCCTGAATGAAGCAGGATATGTACCATCCAATACTGAAAAATATCCTCTTGGAGGCATTGTTTCTGCTATTCAGAATGCTTTCCGGGCAACCCCGAGGTTGGTTTGCAAAAAAGGGGCCTTGGAGGAACTTCATCTATGCTTCTACAAGGATTTCCAGCCTCGGGATTGTCTGGTTGGATCTGGCAGTCTAAGTGACAAGTTAGCTTCAAGTAGCTCATGTCCCAGTTTTGTCAGCATACCAGCATATGCATCATTGGGGCTCGGCGGTGGTGAAGCTGAGATTTCAAGTGCCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004518 GO:0004519 GO:0004521 GO:0004540 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005783 GO:0006139 GO:0006401 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009892 GO:0009894 GO:0009895 GO:0009987 GO:0010168 GO:0010506 GO:0010507 GO:0012505 GO:0016070 GO:0016072 GO:0016075 GO:0016787 GO:0016788 GO:0019222 GO:0019439 GO:0031323 GO:0031324 GO:0031329 GO:0031330 GO:0034641 GO:0034655 GO:0034660 GO:0034661 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044248 GO:0044260 GO:0044265 GO:0044270 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0046700 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0065007 GO:0071704 GO:0090304 GO:0090305 GO:0090501 GO:0090502 GO:0140098 GO:1901360 GO:1901361 GO:1901575

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Ribonuclease_T2

Protein Analysis

274

Amino Acids

29.34

Weight (kDa)

6.63

Isoelectric Point (pI)

39.9

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Ribonuclease_T2	PF00445	46 - 232	1.5e-46	Ribonuclease T2 family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0012525)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G39780 AT2G39780
fragaria_vesca	FvH4_2g17310 FvH4_2g17310
malus_domestica	MD05G1152000.v1.1 MD10G1146300.v1.1
prunus_persica	Prupe.8G184700_v2.0.a1
pyrus_communis	pycom05g14660 pycom10g12620
rosa_chinensis	RchiOBHm_Chr6g0281821
rosa_laevigata	RLG00000012926
rosa_multiflora	Rmu_sc0001992.1_g000009 Rmu_ssc0000126.1_g000039
rosa_roxburghii	Rroxscaffold_7G00186750
rosa_rugosa	Rorug06G0143700 Rorug06G0143800
rosa_samantha	Rh6BG260100 Rh6DG251100
rosa_wichuraiana	Rw6G022260

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB7I	CCANNNNNTGG	1 cut(s)	461
AccII	CGCG	1 cut(s)	222
AciI	CCGC	5 cut(s)	47, 190, 220, 222, 792
AclWI	GGATC	2 cut(s)	418, 712
AcoI	YGGCCR	1 cut(s)	362
AfaI	GTAC	2 cut(s)	512, 549
AfiI	CCNNNNNNNGG	5 cut(s)	162, 461, 578, 625, 688
AgsI	TTSAA	3 cut(s)	148, 735, 813
AjnI	CCWGG	1 cut(s)	166
AluBI	AGCT	4 cut(s)	375, 731, 741, 803
AluI	AGCT	4 cut(s)	375, 731, 741, 803
AlwI	GGATC	2 cut(s)	418, 712
Ama87I	CYCGRG	2 cut(s)	619, 687
AoxI	GGCC	6 cut(s)	153, 164, 263, 287, 362, 642
ApeKI	GCWGC	1 cut(s)	216
Asp700I	GAANNNNTTC	1 cut(s)	605
AspLEI	GCGC	2 cut(s)	56, 186
AspS9I	GGNCC	2 cut(s)	154, 642
AsuC2I	CCSGG	1 cut(s)	611
AsuHPI	GGTGA	2 cut(s)	442, 809
AsuII	TTCGAA	1 cut(s)	99
AvaI	CYCGRG	2 cut(s)	619, 687
BalI	TGGCCA	1 cut(s)	364
BanII	GRGCYC	1 cut(s)	789
BbvI	GCAGC	1 cut(s)	203
BccI	CCATC	4 cut(s)	272, 373, 394, 559
BciT130I	CCWGG	1 cut(s)	168
BcnI	CCSGG	1 cut(s)	611
BfmI	CTRYAG	1 cut(s)	30
BfoI	RGCGCY	1 cut(s)	57
BglII	AGATCT	1 cut(s)	327
BisI	GCNGC	3 cut(s)	217, 220, 223
BlsI	GCNGC	3 cut(s)	218, 221, 224
Bme1390I	CCNGG	2 cut(s)	168, 611
BmeT110I	CYCGRG	2 cut(s)	619, 687
BmgT120I	GGNCC	2 cut(s)	154, 642
BmiI	GGNNCC	1 cut(s)	643
BmrFI	CCNGG	2 cut(s)	168, 611
BmrI	ACTGGG	1 cut(s)	744
BmsI	GCATC	3 cut(s)	64, 334, 785
BmuI	ACTGGG	1 cut(s)	744
BpmI	CTGGAG	1 cut(s)	438
Bpu14I	TTCGAA	1 cut(s)	99
BpuMI	CCSGG	1 cut(s)	611
Bsa29I	ATCGAT	1 cut(s)	72
BsaJI	CCNNGG	6 cut(s)	220, 253, 397, 620, 645, 686
BsaXI	ACNNNNNCTCC	2 cut(s)	570, 600
Bsc4I	CCNNNNNNNGG	5 cut(s)	162, 461, 578, 625, 688
Bse1I	ACTGG	3 cut(s)	365, 455, 750
Bse3DI	GCAATG	2 cut(s)	167, 335
BseBI	CCWGG	1 cut(s)	168
BseCI	ATCGAT	1 cut(s)	72
BseDI	CCNNGG	6 cut(s)	220, 253, 397, 620, 645, 686
BseGI	GGATG	2 cut(s)	389, 551
BseLI	CCNNNNNNNGG	5 cut(s)	162, 461, 578, 625, 688
BseMI	GCAATG	2 cut(s)	167, 335
BseMII	CTCAG	1 cut(s)	795
BseNI	ACTGG	3 cut(s)	365, 455, 750
BseRI	GAGGAG	5 cut(s)	129, 132, 135, 193, 439
BseXI	GCAGC	1 cut(s)	203
Bsh1236I	CGCG	1 cut(s)	222
BshFI	GGCC	6 cut(s)	155, 166, 265, 289, 364, 644
BshVI	ATCGAT	1 cut(s)	72
BsiHKCI	CYCGRG	2 cut(s)	619, 687
BsiSI	CCGG	2 cut(s)	87, 610
BslFI	GGGAC	1 cut(s)	732
BslI	CCNNNNNNNGG	5 cut(s)	162, 461, 578, 625, 688
BsmFI	GGGAC	1 cut(s)	732
BsmI	GAATGC	1 cut(s)	607
BsnI	GGCC	6 cut(s)	155, 166, 265, 289, 364, 644
BsoBI	CYCGRG	2 cut(s)	619, 687
Bsp119I	TTCGAA	1 cut(s)	99
Bsp1286I	GDGCHC	1 cut(s)	789
Bsp143I	GATC	4 cut(s)	69, 327, 410, 704
Bsp19I	CCATGG	2 cut(s)	253, 397
BspACI	CCGC	5 cut(s)	47, 190, 220, 222, 792
BspANI	GGCC	6 cut(s)	155, 166, 265, 289, 364, 644
BspCNI	CTCAG	1 cut(s)	796
BspDI	ATCGAT	1 cut(s)	72
BspFNI	CGCG	1 cut(s)	222
BspHI	TCATGA	1 cut(s)	424
BspLI	GGNNCC	1 cut(s)	643
BspMAI	CTGCAG	1 cut(s)	34
BspPI	GGATC	2 cut(s)	418, 712
BspT104I	TTCGAA	1 cut(s)	99
BsrDI	GCAATG	2 cut(s)	167, 335
BsrI	ACTGG	3 cut(s)	365, 455, 750
BssECI	CCNNGG	6 cut(s)	220, 253, 397, 620, 645, 686
BssMI	GATC	4 cut(s)	69, 327, 410, 704
BssT1I	CCWWGG	3 cut(s)	253, 397, 645
Bst2UI	CCWGG	1 cut(s)	168
BstAPI	GCANNNNNTGC	1 cut(s)	44
BstBI	TTCGAA	1 cut(s)	99
BstC8I	GCNNGC	3 cut(s)	84, 214, 291
BstDEI	CTNAG	2 cut(s)	716, 804
BstDSI	CCRYGG	3 cut(s)	220, 253, 397
BstENI	CCTNNNNNAGG	1 cut(s)	576
BstF5I	GGATG	2 cut(s)	389, 551
BstFNI	CGCG	1 cut(s)	222
BstH2I	RGCGCY	1 cut(s)	57
BstHHI	GCGC	2 cut(s)	56, 186
BstKTI	GATC	4 cut(s)	72, 330, 413, 707
BstMBI	GATC	4 cut(s)	69, 327, 410, 704
BstMWI	GCNNNNNNNGC	7 cut(s)	23, 44, 53, 83, 198, 222, 611
BstNI	CCWGG	1 cut(s)	168
BstSCI	CCNGG	2 cut(s)	166, 609
BstSFI	CTRYAG	1 cut(s)	30
BstUI	CGCG	1 cut(s)	222
BstV1I	GCAGC	1 cut(s)	203
BstX2I	RGATCY	2 cut(s)	327, 704
BstYI	RGATCY	2 cut(s)	327, 704
Bsu15I	ATCGAT	1 cut(s)	72
BsuRI	GGCC	6 cut(s)	155, 166, 265, 289, 364, 644
BsuTUI	ATCGAT	1 cut(s)	72
BtgI	CCRYGG	3 cut(s)	220, 253, 397
BtsCI	GGATG	2 cut(s)	389, 551
BtsIMutI	CAGTG	1 cut(s)	38
Cac8I	GCNNGC	3 cut(s)	84, 214, 291
CciI	TCATGA	1 cut(s)	424
CfoI	GCGC	2 cut(s)	56, 186
Cfr13I	GGNCC	2 cut(s)	154, 642
Cfr42I	CCGCGG	1 cut(s)	223
ClaI	ATCGAT	1 cut(s)	72
Csp6I	GTAC	2 cut(s)	511, 548
CviAII	CATG	5 cut(s)	241, 254, 398, 425, 744
CviQI	GTAC	2 cut(s)	511, 548
DdeI	CTNAG	2 cut(s)	716, 804
DpnI	GATC	4 cut(s)	71, 329, 412, 706
DpnII	GATC	4 cut(s)	69, 327, 410, 704
EaeI	YGGCCR	1 cut(s)	362
Eco130I	CCWWGG	3 cut(s)	253, 397, 645
Eco24I	GRGCYC	1 cut(s)	789
Eco88I	CYCGRG	2 cut(s)	619, 687
EcoNI	CCTNNNNNAGG	1 cut(s)	576
EcoO109I	RGGNCCY	1 cut(s)	642
EcoRII	CCWGG	1 cut(s)	166
EcoT14I	CCWWGG	3 cut(s)	253, 397, 645
EcoT22I	ATGCAT	1 cut(s)	778
EcoT38I	GRGCYC	1 cut(s)	789
ErhI	CCWWGG	3 cut(s)	253, 397, 645
FaeI	CATG	5 cut(s)	244, 257, 401, 428, 747
FaqI	GGGAC	1 cut(s)	732
FatI	CATG	5 cut(s)	240, 253, 397, 424, 743
FauI	CCCGC	1 cut(s)	197
FauNDI	CATATG	1 cut(s)	772
Fnu4HI	GCNGC	3 cut(s)	217, 220, 223
FokI	GGATG	2 cut(s)	376, 538
FriOI	GRGCYC	1 cut(s)	789
Fsp4HI	GCNGC	3 cut(s)	217, 220, 223
GlaI	GCGC	2 cut(s)	55, 185
GluI	GCNGC	3 cut(s)	217, 220, 223
GsuI	CTGGAG	1 cut(s)	438
HaeII	RGCGCY	1 cut(s)	57
HaeIII	GGCC	6 cut(s)	155, 166, 265, 289, 364, 644
HapII	CCGG	2 cut(s)	87, 610
HhaI	GCGC	2 cut(s)	56, 186
Hin1II	CATG	5 cut(s)	244, 257, 401, 428, 747
Hin6I	GCGC	2 cut(s)	54, 184
HinP1I	GCGC	2 cut(s)	54, 184
HinfI	GANTC	2 cut(s)	90, 96
HpaII	CCGG	2 cut(s)	87, 610
HphI	GGTGA	2 cut(s)	442, 809
Hpy166II	GTNNAC	2 cut(s)	246, 383
Hpy188I	TCNGA	3 cut(s)	95, 107, 600
Hpy188III	TCNNGA	3 cut(s)	425, 529, 689
Hpy8I	GTNNAC	2 cut(s)	246, 383
HpyCH4V	TGCA	6 cut(s)	32, 160, 300, 340, 633, 776
HpyF10VI	GCNNNNNNNGC	7 cut(s)	23, 44, 53, 83, 198, 222, 611
HpyF3I	CTNAG	2 cut(s)	716, 804
Hsp92II	CATG	5 cut(s)	244, 257, 401, 428, 747
HspAI	GCGC	2 cut(s)	54, 184
KspI	CCGCGG	1 cut(s)	223
Kzo9I	GATC	4 cut(s)	69, 327, 410, 704
LmnI	GCTCC	3 cut(s)	206, 238, 452
Lsp1109I	GCAGC	1 cut(s)	203
LweI	GCATC	3 cut(s)	64, 334, 785
MaeIII	GTNAC	2 cut(s)	517, 719
MalI	GATC	4 cut(s)	71, 329, 412, 706
MboI	GATC	4 cut(s)	69, 327, 410, 704
MflI	RGATCY	2 cut(s)	327, 704
MhlI	GDGCHC	1 cut(s)	789
MlsI	TGGCCA	1 cut(s)	364
MluCI	AATT	2 cut(s)	20, 475
MluNI	TGGCCA	1 cut(s)	364
MmeI	TCCRAC	3 cut(s)	260, 442, 682
Mox20I	TGGCCA	1 cut(s)	364
Mph1103I	ATGCAT	1 cut(s)	778
MroXI	GAANNNNTTC	1 cut(s)	605
MscI	TGGCCA	1 cut(s)	364
MseI	TTAA	2 cut(s)	371, 507
Msp20I	TGGCCA	1 cut(s)	364
MspA1I	CMGCKG	1 cut(s)	222
MspI	CCGG	2 cut(s)	87, 610
MspR9I	CCNGG	2 cut(s)	168, 611
Mva1269I	GAATGC	1 cut(s)	607
MvaI	CCWGG	1 cut(s)	168
MvnI	CGCG	1 cut(s)	222
MwoI	GCNNNNNNNGC	7 cut(s)	23, 44, 53, 83, 198, 222, 611
NciI	CCSGG	1 cut(s)	611
NcoI	CCATGG	2 cut(s)	253, 397
NdeI	CATATG	1 cut(s)	772
NdeII	GATC	4 cut(s)	69, 327, 410, 704
NlaIII	CATG	5 cut(s)	244, 257, 401, 428, 747
NlaIV	GGNNCC	1 cut(s)	643
NmeAIII	GCCGAG	1 cut(s)	768
NmuCI	GTSAC	2 cut(s)	517, 719
NsiI	ATGCAT	1 cut(s)	778
NspV	TTCGAA	1 cut(s)	99
PagI	TCATGA	1 cut(s)	424
PctI	GAATGC	1 cut(s)	607
PdmI	GAANNNNTTC	1 cut(s)	605
PfeI	GAWTC	2 cut(s)	90, 96
PflMI	CCANNNNNTGG	1 cut(s)	461
PkrI	GCNGC	3 cut(s)	218, 221, 224
Psp6I	CCWGG	1 cut(s)	166
PspGI	CCWGG	1 cut(s)	166
PspN4I	GGNNCC	1 cut(s)	643
PspPI	GGNCC	2 cut(s)	154, 642
PstI	CTGCAG	1 cut(s)	34
PsuI	RGATCY	2 cut(s)	327, 704
RsaI	GTAC	2 cut(s)	512, 549
RsaNI	GTAC	2 cut(s)	511, 548
SacII	CCGCGG	1 cut(s)	223
SaqAI	TTAA	2 cut(s)	371, 507
SatI	GCNGC	3 cut(s)	217, 220, 223
Sau3AI	GATC	4 cut(s)	69, 327, 410, 704
Sau96I	GGNCC	2 cut(s)	154, 642
ScrFI	CCNGG	2 cut(s)	168, 611
SduI	GDGCHC	1 cut(s)	789
SetI	ASST	7 cut(s)	314, 377, 432, 626, 733, 743, 805
SfaNI	GCATC	3 cut(s)	64, 334, 785
SfcI	CTRYAG	1 cut(s)	30
Sfr303I	CCGCGG	1 cut(s)	223
SfuI	TTCGAA	1 cut(s)	99
SgrBI	CCGCGG	1 cut(s)	223
Sse9I	AATT	2 cut(s)	20, 475
SsiI	CCGC	5 cut(s)	47, 190, 220, 222, 792
StyD4I	CCNGG	2 cut(s)	166, 609
StyI	CCWWGG	3 cut(s)	253, 397, 645
TaqI	TCGA	3 cut(s)	72, 99, 138
TasI	AATT	2 cut(s)	20, 475
TatI	WGTACW	1 cut(s)	510
TauI	GCSGC	2 cut(s)	222, 225
TfiI	GAWTC	2 cut(s)	90, 96
Tru1I	TTAA	2 cut(s)	371, 507
Tru9I	TTAA	2 cut(s)	371, 507
TscAI	CASTG	1 cut(s)	45
TseFI	GTSAC	2 cut(s)	517, 719
TseI	GCWGC	1 cut(s)	216
Tsp45I	GTSAC	2 cut(s)	517, 719
TspDTI	ATGAA	3 cut(s)	483, 549, 647
TspGWI	ACGGA	2 cut(s)	319, 453
TspRI	CASTG	1 cut(s)	45
Van91I	CCANNNNNTGG	1 cut(s)	461
XagI	CCTNNNNNAGG	1 cut(s)	576
XmnI	GAANNNNTTC	1 cut(s)	605
Zsp2I	ATGCAT	1 cut(s)	778

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.