Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0012525

Physical Location & Seq

Genomic Coordinates Reverse (-)

23251440 .. 23255071

Gene Structure 3632 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1146300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 819 bp

ATGGCTATCCTCTCTGCTCAATTCGCCTCTGCAGTAGCACTGTTTGCGGCGGCTTCGCTGTGCCTGATCGAAGCCAAGCAAGTCGGAATCGGAGTCGAAATCGGAAGCAGAGGAGGAGGAGGGCAGAGGGAATTCGATTACTTCAATTTGGCCCTGCAATGGCCTGGCACTTTCTGTCAGCGCACCCGCTATTGTTGCTCCTCCAATGCTTGCTGCCGCGGCTCAAATGGTCCAACTGTGTTTACAATCCATGGATTGTGGCCTGACTACAATGATGGAACCTGGCCTGCCTGTTGCACACAGAAAACCTTTGATGAGAAAGAGATCTCAACATTGCACGATGCTTTAGAGAAATACTGGCCGTCTTTAAGCTGTGGTAAACCATCATCCTGCCGTGGTGGAAAAGGATCATTTTGGGGTCATGAGGTGAAGCACGGGACTTGCTCCTCTCCAGTAGTTGGAGATGAATACAATTACTTTTTGACAACCCTCAATGTCTATTTTAAGTACAATGTCACTCAAATCCTGAATGAAGCAGGATACGTACCTTCCAATACCGAAAAGTATCCACTTGGAGGCATTGTTTCTGCTATTCAGAATGTTTTCCGGGCAACCCCCAAGTTGGTTTGCAAAAAAGGTGCTGTGGAGGAACTTCATTTATGCTTCTACAAGGATTTCAAGCCTCGGGATTGTCTGGTTGGATCTGGCAATCTAAATGACAAGTTAGCTTCAAGTAGCTCATGTCCCAATTATGTCAGCATACCAGCATATACATCATGGGGGCTCGGCGGTGGTGAAACTGAGATTTCAAGTGCCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004518 GO:0004519 GO:0004521 GO:0004540 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005783 GO:0006139 GO:0006401 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009892 GO:0009894 GO:0009895 GO:0009987 GO:0010168 GO:0010506 GO:0010507 GO:0012505 GO:0016070 GO:0016072 GO:0016075 GO:0016787 GO:0016788 GO:0019222 GO:0019439 GO:0031323 GO:0031324 GO:0031329 GO:0031330 GO:0034641 GO:0034655 GO:0034660 GO:0034661 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044248 GO:0044260 GO:0044265 GO:0044270 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0046700 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0065007 GO:0071704 GO:0090304 GO:0090305 GO:0090501 GO:0090502 GO:0140098 GO:1901360 GO:1901361 GO:1901575

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Ribonuclease_T2

Protein Analysis

273

Amino Acids

29.48

Weight (kDa)

6.91

Isoelectric Point (pI)

34.87

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Ribonuclease_T2	PF00445	45 - 231	1.4e-45	Ribonuclease T2 family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0012525)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G39780 AT2G39780
fragaria_vesca	FvH4_2g17310 FvH4_2g17310
malus_domestica	MD05G1152000.v1.1 MD10G1146300.v1.1
prunus_persica	Prupe.8G184700_v2.0.a1
pyrus_communis	pycom05g14660 pycom10g12620
rosa_chinensis	RchiOBHm_Chr6g0281821
rosa_laevigata	RLG00000012926
rosa_multiflora	Rmu_sc0001992.1_g000009 Rmu_ssc0000126.1_g000039
rosa_roxburghii	Rroxscaffold_7G00186750
rosa_rugosa	Rorug06G0143700 Rorug06G0143800
rosa_samantha	Rh6BG260100 Rh6DG251100
rosa_wichuraiana	Rw6G022260

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB7I	CCANNNNNTGG	1 cut(s)	458
AccII	CGCG	1 cut(s)	219
AciI	CCGC	6 cut(s)	47, 50, 187, 217, 219, 789
AclWI	GGATC	2 cut(s)	415, 709
AcoI	YGGCCR	1 cut(s)	359
AcsI	RAATTY	1 cut(s)	131
AfaI	GTAC	2 cut(s)	509, 546
AfiI	CCNNNNNNNGG	4 cut(s)	159, 458, 575, 622
AgsI	TTSAA	4 cut(s)	145, 679, 732, 810
AjnI	CCWGG	2 cut(s)	163, 281
AluBI	AGCT	3 cut(s)	372, 728, 738
AluI	AGCT	3 cut(s)	372, 728, 738
AlwI	GGATC	2 cut(s)	415, 709
Ama87I	CYCGRG	1 cut(s)	684
AoxI	GGCC	5 cut(s)	150, 161, 260, 284, 359
ApeKI	GCWGC	1 cut(s)	213
ApoI	RAATTY	1 cut(s)	131
Asp700I	GAANNNNTTC	1 cut(s)	602
AspLEI	GCGC	1 cut(s)	183
AspS9I	GGNCC	2 cut(s)	151, 230
AsuC2I	CCSGG	1 cut(s)	608
AsuHPI	GGTGA	2 cut(s)	439, 806
AvaI	CYCGRG	1 cut(s)	684
AvaII	GGWCC	1 cut(s)	230
BanII	GRGCYC	1 cut(s)	786
BbvI	GCAGC	1 cut(s)	200
BccI	CCATC	2 cut(s)	269, 391
BceAI	ACGGC	2 cut(s)	346, 378
BciT130I	CCWGG	2 cut(s)	165, 283
BciVI	GTATCC	2 cut(s)	533, 576
BcnI	CCSGG	1 cut(s)	608
BfmI	CTRYAG	1 cut(s)	30
BfuI	GTATCC	2 cut(s)	533, 576
BglII	AGATCT	1 cut(s)	324
BisI	GCNGC	5 cut(s)	48, 51, 214, 217, 220
BlsI	GCNGC	5 cut(s)	49, 52, 215, 218, 221
Bme1390I	CCNGG	3 cut(s)	165, 283, 608
Bme18I	GGWCC	1 cut(s)	230
BmeT110I	CYCGRG	1 cut(s)	684
BmgT120I	GGNCC	2 cut(s)	151, 230
BmiI	GGNNCC	1 cut(s)	280
BmrFI	CCNGG	3 cut(s)	165, 283, 608
BmsI	GCATC	1 cut(s)	331
BpmI	CTGGAG	1 cut(s)	435
BpuMI	CCSGG	1 cut(s)	608
BsaAI	YACGTR	1 cut(s)	544
BsaJI	CCNNGG	4 cut(s)	217, 250, 394, 683
BsaXI	ACNNNNNCTCC	2 cut(s)	567, 597
Bsc4I	CCNNNNNNNGG	4 cut(s)	159, 458, 575, 622
Bse1I	ACTGG	2 cut(s)	362, 452
Bse3DI	GCAATG	2 cut(s)	164, 332
BseBI	CCWGG	2 cut(s)	165, 283
BseDI	CCNNGG	4 cut(s)	217, 250, 394, 683
BseGI	GGATG	1 cut(s)	386
BseLI	CCNNNNNNNGG	4 cut(s)	159, 458, 575, 622
BseMI	GCAATG	2 cut(s)	164, 332
BseMII	CTCAG	1 cut(s)	792
BseNI	ACTGG	2 cut(s)	362, 452
BseRI	GAGGAG	5 cut(s)	126, 129, 132, 190, 436
BseXI	GCAGC	1 cut(s)	200
Bsh1236I	CGCG	1 cut(s)	219
BshFI	GGCC	5 cut(s)	152, 163, 262, 286, 361
BsiHKCI	CYCGRG	1 cut(s)	684
BsiSI	CCGG	1 cut(s)	607
BslFI	GGGAC	2 cut(s)	451, 729
BslI	CCNNNNNNNGG	4 cut(s)	159, 458, 575, 622
BsmFI	GGGAC	2 cut(s)	451, 729
BsnI	GGCC	5 cut(s)	152, 163, 262, 286, 361
BsoBI	CYCGRG	1 cut(s)	684
Bsp1286I	GDGCHC	1 cut(s)	786
Bsp143I	GATC	4 cut(s)	66, 324, 407, 701
Bsp19I	CCATGG	1 cut(s)	250
BspACI	CCGC	6 cut(s)	47, 50, 187, 217, 219, 789
BspANI	GGCC	5 cut(s)	152, 163, 262, 286, 361
BspCNI	CTCAG	1 cut(s)	793
BspFNI	CGCG	1 cut(s)	219
BspHI	TCATGA	1 cut(s)	421
BspLI	GGNNCC	1 cut(s)	280
BspMAI	CTGCAG	1 cut(s)	34
BspPI	GGATC	2 cut(s)	415, 709
BsrDI	GCAATG	2 cut(s)	164, 332
BsrI	ACTGG	2 cut(s)	362, 452
BssECI	CCNNGG	4 cut(s)	217, 250, 394, 683
BssMI	GATC	4 cut(s)	66, 324, 407, 701
BssT1I	CCWWGG	1 cut(s)	250
Bst2UI	CCWGG	2 cut(s)	165, 283
Bst4CI	ACNGT	2 cut(s)	42, 238
BstAPI	GCANNNNNTGC	1 cut(s)	44
BstBAI	YACGTR	1 cut(s)	544
BstC8I	GCNNGC	2 cut(s)	211, 288
BstDEI	CTNAG	1 cut(s)	801
BstDSI	CCRYGG	3 cut(s)	217, 250, 394
BstF5I	GGATG	1 cut(s)	386
BstFNI	CGCG	1 cut(s)	219
BstHHI	GCGC	1 cut(s)	183
BstKTI	GATC	4 cut(s)	69, 327, 410, 704
BstMBI	GATC	4 cut(s)	66, 324, 407, 701
BstMWI	GCNNNNNNNGC	4 cut(s)	23, 44, 195, 219
BstNI	CCWGG	2 cut(s)	165, 283
BstSCI	CCNGG	3 cut(s)	163, 281, 606
BstSFI	CTRYAG	1 cut(s)	30
BstSNI	TACGTA	1 cut(s)	544
BstUI	CGCG	1 cut(s)	219
BstV1I	GCAGC	1 cut(s)	200
BstX2I	RGATCY	2 cut(s)	324, 701
BstYI	RGATCY	2 cut(s)	324, 701
BsuI	GTATCC	2 cut(s)	533, 576
BsuRI	GGCC	5 cut(s)	152, 163, 262, 286, 361
BtgI	CCRYGG	3 cut(s)	217, 250, 394
BtsCI	GGATG	1 cut(s)	386
BtsIMutI	CAGTG	1 cut(s)	38
Cac8I	GCNNGC	2 cut(s)	211, 288
CciI	TCATGA	1 cut(s)	421
CfoI	GCGC	1 cut(s)	183
Cfr13I	GGNCC	2 cut(s)	151, 230
Cfr42I	CCGCGG	1 cut(s)	220
Csp6I	GTAC	2 cut(s)	508, 545
CviAII	CATG	4 cut(s)	251, 422, 741, 777
CviQI	GTAC	2 cut(s)	508, 545
DdeI	CTNAG	1 cut(s)	801
DpnI	GATC	4 cut(s)	68, 326, 409, 703
DpnII	GATC	4 cut(s)	66, 324, 407, 701
EaeI	YGGCCR	1 cut(s)	359
Eco105I	TACGTA	1 cut(s)	544
Eco130I	CCWWGG	1 cut(s)	250
Eco24I	GRGCYC	1 cut(s)	786
Eco47I	GGWCC	1 cut(s)	230
Eco88I	CYCGRG	1 cut(s)	684
EcoRI	GAATTC	1 cut(s)	131
EcoRII	CCWGG	2 cut(s)	163, 281
EcoT14I	CCWWGG	1 cut(s)	250
EcoT38I	GRGCYC	1 cut(s)	786
ErhI	CCWWGG	1 cut(s)	250
FaeI	CATG	4 cut(s)	254, 425, 744, 780
FaiI	YATR	9 cut(s)	252, 423, 661, 742, 753, 761, 769, 771, 778
FaqI	GGGAC	2 cut(s)	451, 729
FatI	CATG	4 cut(s)	250, 421, 740, 776
FauI	CCCGC	1 cut(s)	194
Fnu4HI	GCNGC	5 cut(s)	48, 51, 214, 217, 220
FokI	GGATG	1 cut(s)	373
FriOI	GRGCYC	1 cut(s)	786
Fsp4HI	GCNGC	5 cut(s)	48, 51, 214, 217, 220
GlaI	GCGC	1 cut(s)	182
GluI	GCNGC	5 cut(s)	48, 51, 214, 217, 220
GsuI	CTGGAG	1 cut(s)	435
HaeIII	GGCC	5 cut(s)	152, 163, 262, 286, 361
HapII	CCGG	1 cut(s)	607
HhaI	GCGC	1 cut(s)	183
Hin1II	CATG	4 cut(s)	254, 425, 744, 780
Hin6I	GCGC	1 cut(s)	181
HinP1I	GCGC	1 cut(s)	181
HinfI	GANTC	2 cut(s)	87, 93
HpaII	CCGG	1 cut(s)	607
HphI	GGTGA	2 cut(s)	439, 806
Hpy166II	GTNNAC	2 cut(s)	243, 380
Hpy188I	TCNGA	4 cut(s)	86, 92, 104, 597
Hpy188III	TCNNGA	3 cut(s)	422, 526, 686
Hpy8I	GTNNAC	2 cut(s)	243, 380
HpyAV	CCTTC	1 cut(s)	558
HpyCH4III	ACNGT	2 cut(s)	42, 238
HpyCH4IV	ACGT	1 cut(s)	543
HpyCH4V	TGCA	5 cut(s)	32, 157, 297, 337, 630
HpyF10VI	GCNNNNNNNGC	4 cut(s)	23, 44, 195, 219
HpyF3I	CTNAG	1 cut(s)	801
HpySE526I	ACGT	1 cut(s)	543
Hsp92II	CATG	4 cut(s)	254, 425, 744, 780
HspAI	GCGC	1 cut(s)	181
KspI	CCGCGG	1 cut(s)	220
Kzo9I	GATC	4 cut(s)	66, 324, 407, 701
LmnI	GCTCC	2 cut(s)	203, 449
Lsp1109I	GCAGC	1 cut(s)	200
LweI	GCATC	1 cut(s)	331
MaeII	ACGT	1 cut(s)	543
MaeIII	GTNAC	1 cut(s)	514
MalI	GATC	4 cut(s)	68, 326, 409, 703
MboI	GATC	4 cut(s)	66, 324, 407, 701
MflI	RGATCY	2 cut(s)	324, 701
MhlI	GDGCHC	1 cut(s)	786
MluCI	AATT	5 cut(s)	20, 131, 145, 472, 748
MlyI	GAGTC	1 cut(s)	102
MmeI	TCCRAC	4 cut(s)	64, 257, 439, 679
MroXI	GAANNNNTTC	1 cut(s)	602
MseI	TTAA	2 cut(s)	368, 504
MspA1I	CMGCKG	1 cut(s)	219
MspI	CCGG	1 cut(s)	607
MspR9I	CCNGG	3 cut(s)	165, 283, 608
MvaI	CCWGG	2 cut(s)	165, 283
MvnI	CGCG	1 cut(s)	219
MwoI	GCNNNNNNNGC	4 cut(s)	23, 44, 195, 219
NciI	CCSGG	1 cut(s)	608
NcoI	CCATGG	1 cut(s)	250
NdeII	GATC	4 cut(s)	66, 324, 407, 701
NlaIII	CATG	4 cut(s)	254, 425, 744, 780
NlaIV	GGNNCC	1 cut(s)	280
NmeAIII	GCCGAG	1 cut(s)	765
NmuCI	GTSAC	1 cut(s)	514
PagI	TCATGA	1 cut(s)	421
PdmI	GAANNNNTTC	1 cut(s)	602
PfeI	GAWTC	1 cut(s)	87
PflMI	CCANNNNNTGG	1 cut(s)	458
PkrI	GCNGC	5 cut(s)	49, 52, 215, 218, 221
PleI	GAGTC	1 cut(s)	101
PpsI	GAGTC	1 cut(s)	101
Ppu21I	YACGTR	1 cut(s)	544
Psp6I	CCWGG	2 cut(s)	163, 281
PspGI	CCWGG	2 cut(s)	163, 281
PspN4I	GGNNCC	1 cut(s)	280
PspPI	GGNCC	2 cut(s)	151, 230
PstI	CTGCAG	1 cut(s)	34
PsuI	RGATCY	2 cut(s)	324, 701
RsaI	GTAC	2 cut(s)	509, 546
RsaNI	GTAC	2 cut(s)	508, 545
SacII	CCGCGG	1 cut(s)	220
SaqAI	TTAA	2 cut(s)	368, 504
SatI	GCNGC	5 cut(s)	48, 51, 214, 217, 220
Sau3AI	GATC	4 cut(s)	66, 324, 407, 701
Sau96I	GGNCC	2 cut(s)	151, 230
SchI	GAGTC	1 cut(s)	102
ScrFI	CCNGG	3 cut(s)	165, 283, 608
SduI	GDGCHC	1 cut(s)	786
SetI	ASST	9 cut(s)	284, 311, 374, 429, 546, 550, 640, 730, 740
SfaNI	GCATC	1 cut(s)	331
SfcI	CTRYAG	1 cut(s)	30
Sfr303I	CCGCGG	1 cut(s)	220
SgrBI	CCGCGG	1 cut(s)	220
SinI	GGWCC	1 cut(s)	230
SnaBI	TACGTA	1 cut(s)	544
Sse9I	AATT	5 cut(s)	20, 131, 145, 472, 748
SsiI	CCGC	6 cut(s)	47, 50, 187, 217, 219, 789
StyD4I	CCNGG	3 cut(s)	163, 281, 606
StyI	CCWWGG	1 cut(s)	250
TaaI	ACNGT	2 cut(s)	42, 238
TaiI	ACGT	1 cut(s)	546
TaqI	TCGA	3 cut(s)	69, 96, 135
TasI	AATT	5 cut(s)	20, 131, 145, 472, 748
TatI	WGTACW	1 cut(s)	507
TauI	GCSGC	4 cut(s)	50, 53, 219, 222
TfiI	GAWTC	1 cut(s)	87
Tru1I	TTAA	2 cut(s)	368, 504
Tru9I	TTAA	2 cut(s)	368, 504
TscAI	CASTG	1 cut(s)	45
TseFI	GTSAC	1 cut(s)	514
TseI	GCWGC	1 cut(s)	213
Tsp45I	GTSAC	1 cut(s)	514
TspDTI	ATGAA	3 cut(s)	480, 546, 644
TspRI	CASTG	1 cut(s)	45
Van91I	CCANNNNNTGG	1 cut(s)	458
VpaK11BI	GGWCC	1 cut(s)	230
XapI	RAATTY	1 cut(s)	131
XmnI	GAANNNNTTC	1 cut(s)	602

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.