MD05G1164400.v1.1

Protein NRT1 PTR FAMILY

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0007992
Physical Location & Seq
Reverse (-)
29316397 .. 29322734
6338 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1164400.v1.1.491

Sequence Viewer

Length: 1308 bp
ATGGTGTTCTTAGCCAATGCCAGTAACTTCGTTGGATACTTCTTGTTGTCAATGCATTACCCAGATGCTACAGCAGCAAATATGGCCTTCTCGGTCTCAAGCAGCCATTCAGATGTCGTTGCTGCCGATGTAGGTGGAATCAATGCCTCCTTACCTGCACACGGTGCTGATCAGCTTGATCACAGCAACCAGAGACTTATCTCTTCCTACTTCAACTGGTTTTTCTTTTCCCTATGCCTGGGAGGCCTCCTAAGCTGTACTATGATTTGGGTGGAAGAAAACAAAGGCTGGAATTGGAACTTCACTATAACAATTATAGCCTTGAGTTTGGCACCTTCAATTTTCATCGGTGGGAGTCTATTTTATCGACACAAATGTCCTTACGGAAGCGCATTGACAAGAATCTTTAAGTGTCACACTTCAGCACAAATATGTGACCCATCAAATTTTGAGTGGGAAGAAATGGAGCCAGTATTTAAACTCTGCATACAATTTTTTTTCATTTACTTTTGGTCTAAGAGACACTCTAATTTCTCAGATTTTTTAGACAAAGCATTAATTGATAGCACAATTAGTGCCGCTCAAGTTAAGGAAACAAGAGCTTTTATAGGCCTTCTACCCATCTTTGCAAGCACAATCATGATGAATTGCTGCCTAGCCCAACTTCAGACATACTCAGTGGTACAAGGGACCACAATGAACCGAAAAATAAACAATTTCGAAATCCCAGTTCAGTCATTGGGCATACTCCCCCTCTCCGTTTTGCTTGCTTCTATTCCCTTATACGAACGATTCCAAAGAATCTTAGCATCCAAAAGCCCAGCAAAAAGTCACACCTTCCAACCACTTTGGAGGATTGGGCTAGGGCTGGCACTAGCATCTGCATCAATGGCCGTAGCTGCCCTAGTCGAAGGTAAGAGACGCGAGGCAGCTCATAAAAATGCCACATTGTCTTTCTTCTGGCTTGGCTGGCAATACCTCATCCTTGGAGTCTCAGACATCCTCATCCTTGGAGGAATGCTTGAGTTCTTTTACTCGGAAGCACCGGATAGCATGAGAAGCATGTCCACTTCGTTGCCATGGTGCTCAACATCAATGGGGTTCTTTTTAAGCTCATTTCTGGTGTCCATAGCAAATTCAGTGAGTGGAAAATTTGGTAAAGAGTGGCTTGGAGGAGCTGATGGGAACCATTCACGTTTGGATCTGTTTTATACACTCTACGCATTCTCAACACTCTCAATGATGGGCTATGAAGTATTAGTGGAACGCGGTTCTTTCTTACATGCAACTGCACATGTCAAACTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PTR2

Protein Analysis

436

Amino Acids

48.38

Weight (kDa)

7.17

Isoelectric Point (pI)

35.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PTR2 PF00854 10 - 386 1.2e-65 POT family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 375
Acc36I ACCTGC 1 cut(s) 163
AccB1I GGYRCC 1 cut(s) 331
AccBSI CCGCTC 1 cut(s) 581
AccII CGCG 2 cut(s) 924, 1269
AciI CCGC 2 cut(s) 579, 1269
AclWI GGATC 1 cut(s) 1209
AcoI YGGCCR 1 cut(s) 891
AcsI RAATTY 3 cut(s) 445, 1135, 1151
AcuI CTGAAG 2 cut(s) 405, 650
AdeI CACNNNGTG 1 cut(s) 164
AfaI GTAC 2 cut(s) 259, 684
AfiI CCNNNNNNNGG 2 cut(s) 161, 238
AflIII ACRYGT 1 cut(s) 1294
AgsI TTSAA 2 cut(s) 214, 339
AjnI CCWGG 1 cut(s) 237
AluBI AGCT 7 cut(s) 175, 255, 602, 899, 932, 1113, 1178
AluI AGCT 7 cut(s) 175, 255, 602, 899, 932, 1113, 1178
Alw21I GWGCWC 1 cut(s) 1088
Alw26I GTCTC 5 cut(s) 100, 187, 514, 913, 997
AlwI GGATC 1 cut(s) 1209
AoxI GGCC 4 cut(s) 84, 244, 610, 891
ApeKI GCWGC 6 cut(s) 74, 102, 122, 651, 899, 929
ApoI RAATTY 3 cut(s) 445, 1135, 1151
AseI ATTAAT 1 cut(s) 557
AspLEI GCGC 1 cut(s) 392
AspS9I GGNCC 1 cut(s) 690
AsuII TTCGAA 1 cut(s) 720
AvaII GGWCC 1 cut(s) 690
BanI GGYRCC 1 cut(s) 331
Bbv12I GWGCWC 1 cut(s) 1088
BbvI GCAGC 6 cut(s) 86, 109, 114, 638, 886, 941
BccI CCATC 4 cut(s) 448, 629, 1175, 1237
BceAI ACGGC 1 cut(s) 878
BciT130I CCWGG 1 cut(s) 239
BciVI GTATCC 1 cut(s) 29
BclI TGATCA 2 cut(s) 169, 178
BcoDI GTCTC 5 cut(s) 100, 187, 514, 913, 997
BfaI CTAG 4 cut(s) 656, 863, 875, 905
BfmI CTRYAG 1 cut(s) 69
BfuAI ACCTGC 1 cut(s) 163
BfuI GTATCC 1 cut(s) 29
BglI GCCNNNNNGGC 1 cut(s) 243
BisI GCNGC 7 cut(s) 75, 103, 123, 579, 652, 900, 930
BlsI GCNGC 7 cut(s) 76, 104, 124, 580, 653, 901, 931
Bme1390I CCNGG 1 cut(s) 239
Bme18I GGWCC 1 cut(s) 690
BmgT120I GGNCC 1 cut(s) 690
BmiI GGNNCC 4 cut(s) 333, 468, 691, 1187
BmrFI CCNGG 1 cut(s) 239
BmrI ACTGGG 1 cut(s) 722
BmsI GCATC 4 cut(s) 55, 818, 887, 893
BmuI ACTGGG 1 cut(s) 722
Bpu10I CCTNAGC 1 cut(s) 251
Bpu14I TTCGAA 1 cut(s) 720
BpuEI CTTGAG 4 cut(s) 82, 343, 567, 1043
BsaI GGTCTC 1 cut(s) 100
BsaJI CCNNGG 4 cut(s) 238, 985, 1009, 1079
BsaWI WCCGGW 1 cut(s) 1045
Bsc4I CCNNNNNNNGG 2 cut(s) 161, 238
Bse1I ACTGG 4 cut(s) 21, 221, 470, 728
BseBI CCWGG 1 cut(s) 239
BseDI CCNNGG 4 cut(s) 238, 985, 1009, 1079
BseGI GGATG 4 cut(s) 809, 981, 999, 1005
BseLI CCNNNNNNNGG 2 cut(s) 161, 238
BseMII CTCAG 3 cut(s) 549, 690, 1008
BseNI ACTGG 4 cut(s) 21, 221, 470, 728
BseRI GAGGAG 1 cut(s) 1188
BseXI GCAGC 6 cut(s) 86, 109, 114, 638, 886, 941
BseYI CCCAGC 1 cut(s) 820
BsgI GTGCAG 2 cut(s) 141, 1275
Bsh1236I CGCG 2 cut(s) 924, 1269
BshFI GGCC 4 cut(s) 86, 246, 612, 893
BshNI GGYRCC 1 cut(s) 331
BsiHKAI GWGCWC 1 cut(s) 1088
BsiSI CCGG 1 cut(s) 1046
BslFI GGGAC 1 cut(s) 703
BslI CCNNNNNNNGG 2 cut(s) 161, 238
BsmAI GTCTC 5 cut(s) 100, 187, 514, 913, 997
BsmBI CGTCTC 1 cut(s) 913
BsmFI GGGAC 1 cut(s) 703
BsmI GAATGC 2 cut(s) 1023, 1223
BsnI GGCC 4 cut(s) 86, 246, 612, 893
Bso31I GGTCTC 1 cut(s) 100
Bsp119I TTCGAA 1 cut(s) 720
Bsp1286I GDGCHC 1 cut(s) 1088
Bsp143I GATC 3 cut(s) 169, 178, 1201
Bsp19I CCATGG 1 cut(s) 1079
BspACI CCGC 2 cut(s) 579, 1269
BspANI GGCC 4 cut(s) 86, 246, 612, 893
BspCNI CTCAG 3 cut(s) 548, 689, 1007
BspFNI CGCG 2 cut(s) 924, 1269
BspHI TCATGA 1 cut(s) 639
BspLI GGNNCC 4 cut(s) 333, 468, 691, 1187
BspMI ACCTGC 1 cut(s) 163
BspPI GGATC 1 cut(s) 1209
BspT104I TTCGAA 1 cut(s) 720
BspT107I GGYRCC 1 cut(s) 331
BspTNI GGTCTC 1 cut(s) 100
BsrBI CCGCTC 1 cut(s) 581
BsrI ACTGG 4 cut(s) 21, 221, 470, 728
BssECI CCNNGG 4 cut(s) 238, 985, 1009, 1079
BssMI GATC 3 cut(s) 169, 178, 1201
BssT1I CCWWGG 3 cut(s) 985, 1009, 1079
Bst2UI CCWGG 1 cut(s) 239
Bst4CI ACNGT 2 cut(s) 164, 1305
Bst6I CTCTTC 1 cut(s) 208
BstAPI GCANNNNNTGC 1 cut(s) 164
BstBI TTCGAA 1 cut(s) 720
BstC8I GCNNGC 4 cut(s) 631, 768, 870, 971
BstDEI CTNAG 7 cut(s) 10, 251, 516, 535, 676, 805, 994
BstDSI CCRYGG 1 cut(s) 1079
BstF5I GGATG 4 cut(s) 809, 981, 999, 1005
BstFNI CGCG 2 cut(s) 924, 1269
BstHHI GCGC 1 cut(s) 392
BstKTI GATC 3 cut(s) 172, 181, 1204
BstMAI GTCTC 5 cut(s) 100, 187, 514, 913, 997
BstMBI GATC 3 cut(s) 169, 178, 1201
BstMWI GCNNNNNNNGC 9 cut(s) 74, 83, 164, 243, 252, 890, 899, 970, 1059
BstNI CCWGG 1 cut(s) 239
BstNSI RCATGY 3 cut(s) 1066, 1286, 1298
BstSCI CCNGG 1 cut(s) 237
BstSFI CTRYAG 1 cut(s) 69
BstUI CGCG 2 cut(s) 924, 1269
BstV1I GCAGC 6 cut(s) 86, 109, 114, 638, 886, 941
BstX2I RGATCY 1 cut(s) 1201
BstYI RGATCY 1 cut(s) 1201
BsuI GTATCC 1 cut(s) 29
BsuRI GGCC 4 cut(s) 86, 246, 612, 893
BtgI CCRYGG 1 cut(s) 1079
BtsCI GGATG 4 cut(s) 809, 981, 999, 1005
BtsIMutI CAGTG 2 cut(s) 684, 1146
BveI ACCTGC 1 cut(s) 163
Cac8I GCNNGC 4 cut(s) 631, 768, 870, 971
CciI TCATGA 1 cut(s) 639
CfoI GCGC 1 cut(s) 392
Cfr13I GGNCC 1 cut(s) 690
CseI GACGC 1 cut(s) 930
Csp6I GTAC 2 cut(s) 258, 683
CviAII CATG 6 cut(s) 640, 1054, 1063, 1080, 1283, 1295
CviQI GTAC 2 cut(s) 258, 683
DdeI CTNAG 7 cut(s) 10, 251, 516, 535, 676, 805, 994
DpnI GATC 3 cut(s) 171, 180, 1203
DpnII GATC 3 cut(s) 169, 178, 1201
DraI TTTAAA 1 cut(s) 478
DraIII CACNNNGTG 1 cut(s) 164
DrdI GACNNNNNNGTC 1 cut(s) 375
DseDI GACNNNNNNGTC 1 cut(s) 375
EaeI YGGCCR 1 cut(s) 891
Eam1104I CTCTTC 1 cut(s) 208
EarI CTCTTC 1 cut(s) 208
Eco130I CCWWGG 3 cut(s) 985, 1009, 1079
Eco147I AGGCCT 2 cut(s) 246, 612
Eco31I GGTCTC 1 cut(s) 100
Eco47I GGWCC 1 cut(s) 690
Eco57I CTGAAG 2 cut(s) 405, 650
EcoRII CCWGG 1 cut(s) 237
EcoT14I CCWWGG 3 cut(s) 985, 1009, 1079
EcoT22I ATGCAT 1 cut(s) 57
ErhI CCWWGG 3 cut(s) 985, 1009, 1079
Esp3I CGTCTC 1 cut(s) 913
FaeI CATG 6 cut(s) 643, 1057, 1066, 1083, 1286, 1298
FalI AAGNNNNNCTT 2 cut(s) 1152, 1184
FaqI GGGAC 1 cut(s) 703
FatI CATG 6 cut(s) 639, 1053, 1062, 1079, 1282, 1294
FbaI TGATCA 2 cut(s) 169, 178
Fnu4HI GCNGC 7 cut(s) 75, 103, 123, 579, 652, 900, 930
FokI GGATG 4 cut(s) 796, 968, 986, 992
Fsp4HI GCNGC 7 cut(s) 75, 103, 123, 579, 652, 900, 930
FspBI CTAG 4 cut(s) 656, 863, 875, 905
GlaI GCGC 1 cut(s) 391
GluI GCNGC 7 cut(s) 75, 103, 123, 579, 652, 900, 930
GsaI CCCAGC 1 cut(s) 824
HaeIII GGCC 4 cut(s) 86, 246, 612, 893
HapII CCGG 1 cut(s) 1046
HgaI GACGC 1 cut(s) 930
HhaI GCGC 1 cut(s) 392
Hin1II CATG 6 cut(s) 643, 1057, 1066, 1083, 1286, 1298
Hin6I GCGC 1 cut(s) 390
HinP1I GCGC 1 cut(s) 390
HinfI GANTC 6 cut(s) 138, 355, 402, 792, 801, 990
HpaII CCGG 1 cut(s) 1046
Hpy166II GTNNAC 1 cut(s) 1068
Hpy188I TCNGA 5 cut(s) 112, 538, 669, 997, 1039
Hpy188III TCNNGA 1 cut(s) 640
Hpy8I GTNNAC 1 cut(s) 1068
HpyAV CCTTC 5 cut(s) 97, 345, 623, 847, 905
HpyCH4III ACNGT 2 cut(s) 164, 1305
HpyCH4IV ACGT 1 cut(s) 1195
HpyCH4V TGCA 7 cut(s) 55, 158, 486, 629, 884, 1286, 1292
HpyF10VI GCNNNNNNNGC 9 cut(s) 74, 83, 164, 243, 252, 890, 899, 970, 1059
HpyF3I CTNAG 7 cut(s) 10, 251, 516, 535, 676, 805, 994
HpySE526I ACGT 1 cut(s) 1195
Hsp92II CATG 6 cut(s) 643, 1057, 1066, 1083, 1286, 1298
HspAI GCGC 1 cut(s) 390
Ksp22I TGATCA 2 cut(s) 169, 178
Kzo9I GATC 3 cut(s) 169, 178, 1201
LmnI GCTCC 2 cut(s) 466, 1175
Lsp1109I GCAGC 6 cut(s) 86, 109, 114, 638, 886, 941
LweI GCATC 4 cut(s) 55, 818, 887, 893
MaeI CTAG 4 cut(s) 656, 863, 875, 905
MaeII ACGT 1 cut(s) 1195
MaeIII GTNAC 4 cut(s) 23, 413, 434, 830
MalI GATC 3 cut(s) 171, 180, 1203
MbiI CCGCTC 1 cut(s) 581
MboI GATC 3 cut(s) 169, 178, 1201
MboII GAAGA 4 cut(s) 195, 287, 470, 949
MflI RGATCY 1 cut(s) 1201
MhlI GDGCHC 1 cut(s) 1088
MlyI GAGTC 2 cut(s) 364, 999
MmeI TCCRAC 2 cut(s) 13, 865
Mph1103I ATGCAT 1 cut(s) 57
MseI TTAA 5 cut(s) 408, 477, 557, 588, 1109
MslI CAYNNNNRTG 4 cut(s) 111, 430, 638, 939
MspI CCGG 1 cut(s) 1046
MspR9I CCNGG 1 cut(s) 239
Mva1269I GAATGC 2 cut(s) 1023, 1223
MvaI CCWGG 1 cut(s) 239
MvnI CGCG 2 cut(s) 924, 1269
MwoI GCNNNNNNNGC 9 cut(s) 74, 83, 164, 243, 252, 890, 899, 970, 1059
NcoI CCATGG 1 cut(s) 1079
NdeII GATC 3 cut(s) 169, 178, 1201
NlaIII CATG 6 cut(s) 643, 1057, 1066, 1083, 1286, 1298
NlaIV GGNNCC 4 cut(s) 333, 468, 691, 1187
NmuCI GTSAC 3 cut(s) 413, 434, 830
NsiI ATGCAT 1 cut(s) 57
NspI RCATGY 3 cut(s) 1066, 1286, 1298
NspV TTCGAA 1 cut(s) 720
PagI TCATGA 1 cut(s) 639
PceI AGGCCT 2 cut(s) 246, 612
PciI ACATGT 1 cut(s) 1294
PcsI WCGNNNNNNNCGW 1 cut(s) 123
PctI GAATGC 2 cut(s) 1023, 1223
PfeI GAWTC 4 cut(s) 138, 402, 792, 801
PkrI GCNGC 7 cut(s) 76, 104, 124, 580, 653, 901, 931
PleI GAGTC 2 cut(s) 363, 998
PpsI GAGTC 2 cut(s) 363, 998
PscI ACATGT 1 cut(s) 1294
PshBI ATTAAT 1 cut(s) 557
Psp6I CCWGG 1 cut(s) 237
PspFI CCCAGC 1 cut(s) 820
PspGI CCWGG 1 cut(s) 237
PspN4I GGNNCC 4 cut(s) 333, 468, 691, 1187
PspPI GGNCC 1 cut(s) 690
PsuI RGATCY 1 cut(s) 1201
RsaI GTAC 2 cut(s) 259, 684
RsaNI GTAC 2 cut(s) 258, 683
RseI CAYNNNNRTG 4 cut(s) 111, 430, 638, 939
SaqAI TTAA 5 cut(s) 408, 477, 557, 588, 1109
SatI GCNGC 7 cut(s) 75, 103, 123, 579, 652, 900, 930
Sau3AI GATC 3 cut(s) 169, 178, 1201
Sau96I GGNCC 1 cut(s) 690
SchI GAGTC 2 cut(s) 364, 999
ScrFI CCNGG 1 cut(s) 239
SduI GDGCHC 1 cut(s) 1088
SfaNI GCATC 4 cut(s) 55, 818, 887, 893
SfcI CTRYAG 1 cut(s) 69
SfuI TTCGAA 1 cut(s) 720
SinI GGWCC 1 cut(s) 690
SmiMI CAYNNNNRTG 4 cut(s) 111, 430, 638, 939
SmlI CTYRAG 4 cut(s) 97, 322, 582, 1022
SmoI CTYRAG 4 cut(s) 97, 322, 582, 1022
SseBI AGGCCT 2 cut(s) 246, 612
SsiI CCGC 2 cut(s) 579, 1269
SspMI CTAG 4 cut(s) 656, 863, 875, 905
StuI AGGCCT 2 cut(s) 246, 612
StyD4I CCNGG 1 cut(s) 237
StyI CCWWGG 3 cut(s) 985, 1009, 1079
TaaI ACNGT 2 cut(s) 164, 1305
TaiI ACGT 1 cut(s) 1198
TaqI TCGA 3 cut(s) 367, 720, 909
TaqII GACCGA 1 cut(s) 82
TatI WGTACW 1 cut(s) 257
TauI GCSGC 1 cut(s) 581
TfiI GAWTC 4 cut(s) 138, 402, 792, 801
Tru1I TTAA 5 cut(s) 408, 477, 557, 588, 1109
Tru9I TTAA 5 cut(s) 408, 477, 557, 588, 1109
TscAI CASTG 2 cut(s) 684, 1146
TseFI GTSAC 3 cut(s) 413, 434, 830
TseI GCWGC 6 cut(s) 74, 102, 122, 651, 899, 929
Tsp45I GTSAC 3 cut(s) 413, 434, 830
TspDTI ATGAA 5 cut(s) 334, 490, 659, 713, 1266
TspGWI ACGGA 2 cut(s) 399, 748
TspRI CASTG 2 cut(s) 684, 1146
VpaK11BI GGWCC 1 cut(s) 690
VspI ATTAAT 1 cut(s) 557
XapI RAATTY 3 cut(s) 445, 1135, 1151
XceI RCATGY 3 cut(s) 1066, 1286, 1298
XspI CTAG 4 cut(s) 656, 863, 875, 905
Zsp2I ATGCAT 1 cut(s) 57
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.