Rroxscaffold_7G00185560

Protein NRT1 PTR FAMILY

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0007992
Physical Location & Seq
Forward (+)
24987825 .. 24990205
2381 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00185560.1

Sequence Viewer

Length: 1458 bp
ATGAGTATGGAGATAGATACCTACCAGAAAGAAAGTGATGATCAGTATGTTGATTGGAAAGGAAGAAAGGCAAATCCTAAAATCCATGGTGGAATTAGAGCTGCTGCCTTTGCATGTGTTGTTGAGGTCTTTACCAACATGGTTTTCTTATGCAATGCTAGTAACTTTGTCACATACTTCACAAAGTCAATGCATTATCCACCTGCTAAATCAGCAAATATGGGACTAATCTTGCTGACAATCCAATCTCAAAGCAGAAGGTTACGGCCGGCGTTGAATTTAAAGCCTTCTCAGTCTCAAGCAGCAATTCTGTACACCGGCCTTTACGCAATTGCTGCTGGTGCTGCTGGACTCAATGCCAACTTACCTGCACATGGTGCTGATCAGCTTGATCACAGCAATCAGAGACAGATCTCTGCCTTCTTCAATTGGTTTTTCTTTTCCTTATGCACTGGTGGACTCATTAGCTCAACTGTCATGGTTTGGGTGGAAGAGAACAAAGGCTGGAATTGGAGCTTTCCCATAACAGTTATAGTTTTGAGTTTGGCGCTTCTTATCTTCATCAGTGGAAGTCCATTTTATAGGCACAAACGACCCACTGGGAGCTCATTAACAAGGATCATCAAGGTACTTACTTCTGCTGCACAAAACTGGAAAGCCTCACCAGACGAGAGATTGGGGAACCGGTATGCAGTAGCTGCACTCAACCTGAACAGAGCGGATGGAGATCACAAGTTTAAGTTCCTGGACAAAGCATTAATAGACAACAGAGTCAATGCAGCTGAAATTAAGGAAACAAGAACTTTTCTTGGCCTTTTACCTATATTTGCAAGCACAATCATGATGAACTGTTGCCTAGCTCAACTTCAAACATTCTCCGTGGTACAAGGGAGCATAATGAATAGGAAACTACATAACTTTGAAATTCCGACTCCATCATTAACAGTAATCCCTCTTATCATCACGCTATCCTCCATTCCCTTATACGAACGATTACATTCTGGAAAAAACATCATTTTCAAACCACTCTGGAGGATCGGACTGGGGCTAGCTCTAGCATCAGGCTCAATGGCCGTAGCTGCTCTCATCGAAGCTAAGAGGCGTGCAGAGGCTGCTGACAGCAATGTGATATCGGTATTTTGGCTCGGGTGGCAATTCCTGTTGCTAGGAGTGTCTGATATGCTCACTCTTGGAGGAATGCTCGAGTTCTTTTACTCCGAGGCACCCAATAGCATGAGAAGCACGTGCACTGCTTTGTCATGGTGCTCAACATCAATGGGGTACTTCTTGAGCTCAGTGCTGGTGTCCATAGTAAATTCAGTTAGTGGAAAATATGGGAAAGAGTGGCTTGGAGGGATGAATTTGAATCATGGGCGCTTGGATCTGTTTTACACTCTTCTTAGCATTCTCAACTCCCTGAATTTTCTCAACTACATGTATTGGGCAAAGAGGTATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PTR2

Protein Analysis

485

Amino Acids

53.94

Weight (kDa)

9.47

Isoelectric Point (pI)

39.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PTR2 PF00854 74 - 444 1.3e-76 POT family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 211
AasI GACNNNNNNGTC 1 cut(s) 770
Acc36I ACCTGC 2 cut(s) 211, 376
AccB1I GGYRCC 1 cut(s) 1222
AccBSI CCGCTC 1 cut(s) 719
AciI CCGC 1 cut(s) 719
AclWI GGATC 3 cut(s) 626, 1043, 1389
AcoI YGGCCR 2 cut(s) 266, 1071
AcsI RAATTY 5 cut(s) 277, 924, 1315, 1360, 1420
AcvI CACGTG 1 cut(s) 1245
AdeI CACNNNGTG 1 cut(s) 377
AfaI GTAC 4 cut(s) 314, 630, 885, 1283
AfiI CCNNNNNNNGG 1 cut(s) 374
AflIII ACRYGT 1 cut(s) 1434
AgeI ACCGGT 1 cut(s) 684
AgsI TTSAA 6 cut(s) 277, 427, 869, 923, 1021, 1366
AjnI CCWGG 1 cut(s) 744
AjuI GAANNNNNNNTTGG 2 cut(s) 128, 160
Alw21I GWGCWC 4 cut(s) 608, 1250, 1268, 1295
Alw26I GTCTC 2 cut(s) 300, 400
Alw44I GTGCAC 1 cut(s) 1246
AlwI GGATC 3 cut(s) 626, 1043, 1389
AlwNI CAGNNNCTG 2 cut(s) 698, 1112
Ama87I CYCGRG 2 cut(s) 1145, 1202
AoxI GGCC 4 cut(s) 266, 319, 811, 1071
ApaLI GTGCAC 1 cut(s) 1246
ApoI RAATTY 5 cut(s) 277, 924, 1315, 1360, 1420
AseI ATTAAT 1 cut(s) 758
AsiGI ACCGGT 1 cut(s) 684
AspLEI GCGC 2 cut(s) 550, 1377
AsuHPI GGTGA 1 cut(s) 654
AsuNHI GCTAGC 1 cut(s) 1048
AvaI CYCGRG 2 cut(s) 1145, 1202
BaeGI GKGCMC 1 cut(s) 1250
BanI GGYRCC 1 cut(s) 1222
BanII GRGCYC 2 cut(s) 608, 1295
BbrPI CACGTG 1 cut(s) 1245
Bbv12I GWGCWC 4 cut(s) 608, 1250, 1268, 1295
BccI CCATC 2 cut(s) 716, 943
BceAI ACGGC 2 cut(s) 281, 1058
BciT130I CCWGG 1 cut(s) 746
BclI TGATCA 3 cut(s) 40, 382, 391
BcoDI GTCTC 2 cut(s) 300, 400
BfaI CTAG 5 cut(s) 159, 857, 1049, 1055, 1166
BfoI RGCGCY 2 cut(s) 551, 1378
BfuAI ACCTGC 2 cut(s) 211, 376
BglII AGATCT 1 cut(s) 411
Bme1390I CCNGG 1 cut(s) 746
BmeT110I CYCGRG 2 cut(s) 1145, 1202
BmiI GGNNCC 2 cut(s) 683, 1224
BmrFI CCNGG 1 cut(s) 746
BmrI ACTGGG 2 cut(s) 609, 1052
BmsI GCATC 1 cut(s) 1067
BmtI GCTAGC 1 cut(s) 1052
BmuI ACTGGG 2 cut(s) 609, 1052
BplI GAGNNNNNCTC 2 cut(s) 1185, 1217
BpmI CTGGAG 1 cut(s) 1051
BpuEI CTTGAG 2 cut(s) 282, 1309
BsaAI YACGTR 1 cut(s) 1245
BsaBI GATNNNNATC 1 cut(s) 726
BsaJI CCNNGG 3 cut(s) 85, 879, 1218
BsaWI WCCGGW 1 cut(s) 684
Bsc4I CCNNNNNNNGG 1 cut(s) 374
Bse118I RCCGGY 3 cut(s) 268, 317, 684
Bse1I ACTGG 4 cut(s) 457, 604, 656, 1047
Bse3DI GCAATG 2 cut(s) 160, 1129
Bse8I GATNNNNATC 1 cut(s) 726
BseBI CCWGG 1 cut(s) 746
BseDI CCNNGG 3 cut(s) 85, 879, 1218
BseGI GGATG 2 cut(s) 727, 1362
BseJI GATNNNNATC 1 cut(s) 726
BseLI CCNNNNNNNGG 1 cut(s) 374
BseMI GCAATG 2 cut(s) 160, 1129
BseMII CTCAG 2 cut(s) 305, 1308
BseNI ACTGG 4 cut(s) 457, 604, 656, 1047
BseSI GKGCMC 1 cut(s) 1250
BseX3I CGGCCG 1 cut(s) 266
BsgI GTGCAG 4 cut(s) 354, 627, 684, 1125
Bsh1285I CGRYCG 1 cut(s) 269
BshFI GGCC 4 cut(s) 268, 321, 813, 1073
BshNI GGYRCC 1 cut(s) 1222
BshTI ACCGGT 1 cut(s) 684
BsiEI CGRYCG 1 cut(s) 269
BsiHKAI GWGCWC 4 cut(s) 608, 1250, 1268, 1295
BsiHKCI CYCGRG 2 cut(s) 1145, 1202
BsiSI CCGG 3 cut(s) 269, 318, 685
BslFI GGGAC 1 cut(s) 237
BslI CCNNNNNNNGG 1 cut(s) 374
BsmAI GTCTC 2 cut(s) 300, 400
BsmFI GGGAC 1 cut(s) 237
BsmI GAATGC 2 cut(s) 1203, 1404
BsnI GGCC 4 cut(s) 268, 321, 813, 1073
BsoBI CYCGRG 2 cut(s) 1145, 1202
Bsp1286I GDGCHC 4 cut(s) 608, 1250, 1268, 1295
Bsp1407I TGTACA 1 cut(s) 312
Bsp143I GATC 8 cut(s) 40, 382, 391, 411, 618, 727, 1035, 1381
Bsp19I CCATGG 1 cut(s) 85
BspACI CCGC 1 cut(s) 719
BspANI GGCC 4 cut(s) 268, 321, 813, 1073
BspCNI CTCAG 2 cut(s) 304, 1307
BspHI TCATGA 1 cut(s) 840
BspLI GGNNCC 2 cut(s) 683, 1224
BspMI ACCTGC 2 cut(s) 211, 376
BspOI GCTAGC 1 cut(s) 1052
BspPI GGATC 3 cut(s) 626, 1043, 1389
BspT107I GGYRCC 1 cut(s) 1222
BsrBI CCGCTC 1 cut(s) 719
BsrDI GCAATG 2 cut(s) 160, 1129
BsrFI RCCGGY 3 cut(s) 268, 317, 684
BsrGI TGTACA 1 cut(s) 312
BsrI ACTGG 4 cut(s) 457, 604, 656, 1047
BssAI RCCGGY 3 cut(s) 268, 317, 684
BssECI CCNNGG 3 cut(s) 85, 879, 1218
BssMI GATC 8 cut(s) 40, 382, 391, 411, 618, 727, 1035, 1381
BssT1I CCWWGG 1 cut(s) 85
Bst2UI CCWGG 1 cut(s) 746
Bst4CI ACNGT 4 cut(s) 475, 529, 851, 946
Bst6I CTCTTC 2 cut(s) 486, 1401
BstAPI GCANNNNNTGC 4 cut(s) 335, 377, 698, 1112
BstAUI TGTACA 1 cut(s) 312
BstBAI YACGTR 1 cut(s) 1245
BstC8I GCNNGC 4 cut(s) 270, 832, 1050, 1104
BstDEI CTNAG 4 cut(s) 291, 1095, 1294, 1400
BstDSI CCRYGG 2 cut(s) 85, 879
BstF5I GGATG 2 cut(s) 727, 1362
BstH2I RGCGCY 2 cut(s) 551, 1378
BstHHI GCGC 2 cut(s) 550, 1377
BstKTI GATC 8 cut(s) 43, 385, 394, 414, 621, 730, 1038, 1384
BstMAI GTCTC 2 cut(s) 300, 400
BstMBI GATC 8 cut(s) 40, 382, 391, 411, 618, 727, 1035, 1381
BstMCI CGRYCG 1 cut(s) 269
BstNI CCWGG 1 cut(s) 746
BstNSI RCATGY 2 cut(s) 117, 1438
BstSCI CCNGG 1 cut(s) 744
BstSLI GKGCMC 1 cut(s) 1250
BstX2I RGATCY 2 cut(s) 411, 1381
BstYI RGATCY 2 cut(s) 411, 1381
BstZI CGGCCG 1 cut(s) 266
BsuRI GGCC 4 cut(s) 268, 321, 813, 1073
BtgI CCRYGG 2 cut(s) 85, 879
BtsCI GGATG 2 cut(s) 727, 1362
BtsI GCAGTG 1 cut(s) 1248
BtsIMutI CAGTG 5 cut(s) 450, 571, 597, 1248, 1302
BveI ACCTGC 2 cut(s) 211, 376
Cac8I GCNNGC 4 cut(s) 270, 832, 1050, 1104
CaiI CAGNNNCTG 2 cut(s) 698, 1112
CciI TCATGA 1 cut(s) 840
CfoI GCGC 2 cut(s) 550, 1377
Cfr10I RCCGGY 3 cut(s) 268, 317, 684
Csp6I GTAC 4 cut(s) 313, 629, 884, 1282
CspAI ACCGGT 1 cut(s) 684
CviQI GTAC 4 cut(s) 313, 629, 884, 1282
DdeI CTNAG 4 cut(s) 291, 1095, 1294, 1400
DpnI GATC 8 cut(s) 42, 384, 393, 413, 620, 729, 1037, 1383
DpnII GATC 8 cut(s) 40, 382, 391, 411, 618, 727, 1035, 1381
DraI TTTAAA 1 cut(s) 282
DraIII CACNNNGTG 1 cut(s) 377
DrdI GACNNNNNNGTC 1 cut(s) 770
DseDI GACNNNNNNGTC 1 cut(s) 770
EaeI YGGCCR 2 cut(s) 266, 1071
EagI CGGCCG 1 cut(s) 266
Eam1104I CTCTTC 2 cut(s) 486, 1401
EarI CTCTTC 2 cut(s) 486, 1401
Ecl136II GAGCTC 2 cut(s) 606, 1293
EclXI CGGCCG 1 cut(s) 266
Eco130I CCWWGG 1 cut(s) 85
Eco24I GRGCYC 2 cut(s) 608, 1295
Eco32I GATATC 1 cut(s) 1131
Eco52I CGGCCG 1 cut(s) 266
Eco53kI GAGCTC 2 cut(s) 606, 1293
Eco72I CACGTG 1 cut(s) 1245
Eco88I CYCGRG 2 cut(s) 1145, 1202
EcoICRI GAGCTC 2 cut(s) 606, 1293
EcoRII CCWGG 1 cut(s) 744
EcoRV GATATC 1 cut(s) 1131
EcoT14I CCWWGG 1 cut(s) 85
EcoT22I ATGCAT 1 cut(s) 195
EcoT38I GRGCYC 2 cut(s) 608, 1295
ErhI CCWWGG 1 cut(s) 85
FalI AAGNNNNNCTT 2 cut(s) 1332, 1364
FaqI GGGAC 1 cut(s) 237
FbaI TGATCA 3 cut(s) 40, 382, 391
FokI GGATG 2 cut(s) 734, 1369
FriOI GRGCYC 2 cut(s) 608, 1295
FspBI CTAG 5 cut(s) 159, 857, 1049, 1055, 1166
GlaI GCGC 2 cut(s) 549, 1376
GsuI CTGGAG 1 cut(s) 1051
HaeII RGCGCY 2 cut(s) 551, 1378
HaeIII GGCC 4 cut(s) 268, 321, 813, 1073
HapII CCGG 3 cut(s) 269, 318, 685
HhaI GCGC 2 cut(s) 550, 1377
Hin6I GCGC 2 cut(s) 548, 1375
HinP1I GCGC 2 cut(s) 548, 1375
HinfI GANTC 5 cut(s) 351, 459, 771, 931, 1366
HpaII CCGG 3 cut(s) 269, 318, 685
HphI GGTGA 1 cut(s) 654
Hpy166II GTNNAC 3 cut(s) 315, 458, 1248
Hpy188I TCNGA 5 cut(s) 405, 930, 1040, 1177, 1219
Hpy188III TCNNGA 4 cut(s) 841, 1002, 1030, 1288
Hpy8I GTNNAC 3 cut(s) 315, 458, 1248
HpyAV CCTTC 3 cut(s) 252, 297, 430
HpyCH4III ACNGT 4 cut(s) 475, 529, 851, 946
HpyCH4IV ACGT 1 cut(s) 1244
HpyF3I CTNAG 4 cut(s) 291, 1095, 1294, 1400
HpySE526I ACGT 1 cut(s) 1244
HspAI GCGC 2 cut(s) 548, 1375
KroI GCCGGC 1 cut(s) 268
KroNI GCCGGC 1 cut(s) 270
Ksp22I TGATCA 3 cut(s) 40, 382, 391
Kzo9I GATC 8 cut(s) 40, 382, 391, 411, 618, 727, 1035, 1381
LmnI GCTCC 3 cut(s) 513, 603, 891
LweI GCATC 1 cut(s) 1067
MaeI CTAG 5 cut(s) 159, 857, 1049, 1055, 1166
MaeII ACGT 1 cut(s) 1244
MaeIII GTNAC 3 cut(s) 161, 169, 261
MalI GATC 8 cut(s) 42, 384, 393, 413, 620, 729, 1037, 1383
MbiI CCGCTC 1 cut(s) 719
MboI GATC 8 cut(s) 40, 382, 391, 411, 618, 727, 1035, 1381
MboII GAAGA 5 cut(s) 75, 415, 503, 550, 1388
MfeI CAATTG 2 cut(s) 330, 427
MflI RGATCY 2 cut(s) 411, 1381
MhlI GDGCHC 4 cut(s) 608, 1250, 1268, 1295
MlyI GAGTC 4 cut(s) 345, 453, 780, 925
MmeI TCCRAC 1 cut(s) 953
Mph1103I ATGCAT 1 cut(s) 195
MroNI GCCGGC 1 cut(s) 268
MseI TTAA 6 cut(s) 281, 611, 738, 758, 789, 941
MslI CAYNNNNRTG 1 cut(s) 839
MspA1I CMGCKG 1 cut(s) 782
MspI CCGG 3 cut(s) 269, 318, 685
MspR9I CCNGG 1 cut(s) 746
MunI CAATTG 2 cut(s) 330, 427
Mva1269I GAATGC 2 cut(s) 1203, 1404
MvaI CCWGG 1 cut(s) 746
NaeI GCCGGC 1 cut(s) 270
NcoI CCATGG 1 cut(s) 85
NdeII GATC 8 cut(s) 40, 382, 391, 411, 618, 727, 1035, 1381
NgoMIV GCCGGC 1 cut(s) 268
NheI GCTAGC 1 cut(s) 1048
NlaIV GGNNCC 2 cut(s) 683, 1224
NmuCI GTSAC 1 cut(s) 169
NsiI ATGCAT 1 cut(s) 195
NspI RCATGY 2 cut(s) 117, 1438
PaeR7I CTCGAG 1 cut(s) 1202
PagI TCATGA 1 cut(s) 840
PaqCI CACCTGC 1 cut(s) 211
PciI ACATGT 1 cut(s) 1434
PctI GAATGC 2 cut(s) 1203, 1404
PdiI GCCGGC 1 cut(s) 270
PfeI GAWTC 1 cut(s) 1366
PfoI TCCNGGA 1 cut(s) 744
PinAI ACCGGT 1 cut(s) 684
PleI GAGTC 4 cut(s) 345, 453, 779, 925
PmaCI CACGTG 1 cut(s) 1245
PmlI CACGTG 1 cut(s) 1245
PpsI GAGTC 4 cut(s) 345, 453, 779, 925
Ppu21I YACGTR 1 cut(s) 1245
PscI ACATGT 1 cut(s) 1434
PshBI ATTAAT 1 cut(s) 758
Psp124BI GAGCTC 2 cut(s) 608, 1295
Psp6I CCWGG 1 cut(s) 744
PspCI CACGTG 1 cut(s) 1245
PspGI CCWGG 1 cut(s) 744
PspN4I GGNNCC 2 cut(s) 683, 1224
PspXI VCTCGAGB 1 cut(s) 1202
PstNI CAGNNNCTG 2 cut(s) 698, 1112
PsuI RGATCY 2 cut(s) 411, 1381
PvuII CAGCTG 1 cut(s) 782
RsaI GTAC 4 cut(s) 314, 630, 885, 1283
RsaNI GTAC 4 cut(s) 313, 629, 884, 1282
RseI CAYNNNNRTG 1 cut(s) 839
SacI GAGCTC 2 cut(s) 608, 1295
SaqAI TTAA 6 cut(s) 281, 611, 738, 758, 789, 941
Sau3AI GATC 8 cut(s) 40, 382, 391, 411, 618, 727, 1035, 1381
SchI GAGTC 4 cut(s) 345, 453, 780, 925
ScrFI CCNGG 1 cut(s) 746
SduI GDGCHC 4 cut(s) 608, 1250, 1268, 1295
SfaNI GCATC 1 cut(s) 1067
Sfr274I CTCGAG 1 cut(s) 1202
SlaI CTCGAG 1 cut(s) 1202
SmiMI CAYNNNNRTG 1 cut(s) 839
SmlI CTYRAG 3 cut(s) 297, 1202, 1288
SmoI CTYRAG 3 cut(s) 297, 1202, 1288
SsiI CCGC 1 cut(s) 719
SspMI CTAG 5 cut(s) 159, 857, 1049, 1055, 1166
SstI GAGCTC 2 cut(s) 608, 1295
StyD4I CCNGG 1 cut(s) 744
StyI CCWWGG 1 cut(s) 85
TaaI ACNGT 4 cut(s) 475, 529, 851, 946
TaiI ACGT 1 cut(s) 1247
TaqI TCGA 2 cut(s) 1089, 1203
TatI WGTACW 1 cut(s) 312
TfiI GAWTC 1 cut(s) 1366
Tru1I TTAA 6 cut(s) 281, 611, 738, 758, 789, 941
Tru9I TTAA 6 cut(s) 281, 611, 738, 758, 789, 941
TscAI CASTG 5 cut(s) 457, 571, 604, 1255, 1302
TseFI GTSAC 1 cut(s) 169
Tsp45I GTSAC 1 cut(s) 169
TspDTI ATGAA 4 cut(s) 550, 860, 914, 1373
TspGWI ACGGA 1 cut(s) 868
TspRI CASTG 5 cut(s) 457, 571, 604, 1255, 1302
VneI GTGCAC 1 cut(s) 1246
VspI ATTAAT 1 cut(s) 758
XapI RAATTY 5 cut(s) 277, 924, 1315, 1360, 1420
XceI RCATGY 2 cut(s) 117, 1438
XhoI CTCGAG 1 cut(s) 1202
XspI CTAG 5 cut(s) 159, 857, 1049, 1055, 1166
Zsp2I ATGCAT 1 cut(s) 195
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.