Rorug06G0153600

Protein NRT1 PTR FAMILY

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0007992
Physical Location & Seq
Reverse (-)
22062522 .. 22065995
3474 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0153600.1

Sequence Viewer

Length: 1014 bp
ATGGAGGTGGGTCTTGTTTCGTTGCTGAGAACAGCTTGGATTGCAGCGACATTGCCACTACTCATAGCTTCGTTGCCCTTTTCTTGGCTCAACCCTGTTCATAAAACCGTTTTGGGGTTTGCCAAGAGAGGGAAGACCATGTCTTCTTCAAGGAGATTCACAGTTCCTCAAAAGTTCTTCTGCCATTTCTATGTGCTGGCTGTCGTGTGGACAACCTTACTTCTTGTTACAACTTGGATGTATGCATATAAAATGGTGCCAATGTATGTGGACCCATTTCTTTATCCTACGTTCGCCAGCCGCTTTACTGGAGGTTCACATATCCTTTCCTGGCGCAAGTCTCCATCAGTTCCTATGGGTTATAGATATAGTGTCTGGCGTTCTGTCTTTTTGCTTTTGTTGATGGAAGTTCAAGTTGTGAACCGCCTTATTGAGACAATATATGTATTCAAATATAGCTCCACTGCTAGGATGCACATCTTTGGGTATCTGACTGGCCTCTTTTTCTACACAGCTGCACCCTTGTCACTTTGCTGCAATAATTTTCTGGAGGTCTATAACTTTTCCGTTAATGGAGTGGCTGAATTTATTGTCAAAGGCAAGAAGAATACTATGGAAGATATGGATTTTGATTCTTTGGAATTTATTAGTTCTCTTCTGAAGCTTGGTTGGCTCCAGTGGATTGGTGCTGCTATATTCTTTTGGGGTTGGATCCATCAGCGCCGCTGTCATGAAATTCTTGGCTCATTACGAGAACACAGTGAACAAAATGTATATGTGATTCCTCATGGGGACTGGTTTAAAATTGTATCATCTCCACATTATCTTGCTGAGATAGTTATATATGCTGGTCTTGTGGTTGCTAGTGGAGGAGCAGACCTCACAATCTGGTTACTATTTGGATTCGTGTTGTCAAACTTGGTGTTTGCAGCAGCAGAAACACATAGATGGTACCTTCAAAAGTTCAAAAGTTACCCCAAAAATCGGCGTGCCATTATTCCAATTTTATACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PTR2

Protein Analysis

337

Amino Acids

38.92

Weight (kDa)

9.56

Isoelectric Point (pI)

48.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Steroid_dh PF02544 226 - 337 6.6e-26 3-oxo-5-alpha-steroid 4-dehydrogenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 951
AccB1I GGYRCC 2 cut(s) 256, 951
AciI CCGC 3 cut(s) 301, 424, 724
AclWI GGATC 2 cut(s) 706, 719
AcsI RAATTY 3 cut(s) 584, 641, 735
AcuI CTGAAG 1 cut(s) 680
AfaI GTAC 1 cut(s) 953
AfiI CCNNNNNNNGG 5 cut(s) 84, 114, 129, 468, 984
AgsI TTSAA 5 cut(s) 150, 413, 451, 959, 967
AjnI CCWGG 1 cut(s) 329
AluBI AGCT 5 cut(s) 35, 68, 459, 515, 664
AluI AGCT 5 cut(s) 35, 68, 459, 515, 664
Alw26I GTCTC 2 cut(s) 345, 428
AlwI GGATC 2 cut(s) 706, 719
AoxI GGCC 1 cut(s) 496
ApeKI GCWGC 6 cut(s) 44, 515, 534, 689, 929, 932
ApoI RAATTY 3 cut(s) 584, 641, 735
Asp718I GGTACC 1 cut(s) 951
AspLEI GCGC 2 cut(s) 336, 723
AspS9I GGNCC 1 cut(s) 271
AvaII GGWCC 1 cut(s) 271
BamHI GGATCC 1 cut(s) 711
BanI GGYRCC 2 cut(s) 256, 951
BbsI GAAGAC 2 cut(s) 135, 140
BbvI GCAGC 6 cut(s) 56, 502, 521, 676, 941, 944
BccI CCATC 4 cut(s) 352, 397, 723, 942
BciT130I CCWGG 1 cut(s) 331
BcoDI GTCTC 2 cut(s) 345, 428
BfaI CTAG 2 cut(s) 468, 864
BfoI RGCGCY 1 cut(s) 724
BisI GCNGC 8 cut(s) 45, 301, 516, 535, 690, 724, 930, 933
BlsI GCNGC 8 cut(s) 46, 302, 517, 536, 691, 725, 931, 934
Bme1390I CCNGG 1 cut(s) 331
Bme18I GGWCC 1 cut(s) 271
BmgT120I GGNCC 1 cut(s) 271
BmiI GGNNCC 5 cut(s) 258, 273, 674, 713, 953
BmrFI CCNGG 1 cut(s) 331
BmsI GCATC 1 cut(s) 462
BpiI GAAGAC 2 cut(s) 135, 140
BplI GAGNNNNNCTC 2 cut(s) 864, 896
BpmI CTGGAG 3 cut(s) 330, 569, 659
BsaBI GATNNNNATC 1 cut(s) 476
Bsc4I CCNNNNNNNGG 5 cut(s) 84, 114, 129, 468, 984
Bse1I ACTGG 4 cut(s) 313, 499, 676, 800
Bse3DI GCAATG 1 cut(s) 50
Bse8I GATNNNNATC 1 cut(s) 476
BseBI CCWGG 1 cut(s) 331
BseGI GGATG 2 cut(s) 243, 477
BseJI GATNNNNATC 1 cut(s) 476
BseLI CCNNNNNNNGG 5 cut(s) 84, 114, 129, 468, 984
BseMI GCAATG 1 cut(s) 50
BseMII CTCAG 2 cut(s) 17, 822
BseNI ACTGG 4 cut(s) 313, 499, 676, 800
BseRI GAGGAG 1 cut(s) 885
BseXI GCAGC 6 cut(s) 56, 502, 521, 676, 941, 944
BsgI GTGCAG 1 cut(s) 501
BshFI GGCC 1 cut(s) 498
BshNI GGYRCC 2 cut(s) 256, 951
BslFI GGGAC 1 cut(s) 806
BslI CCNNNNNNNGG 5 cut(s) 84, 114, 129, 468, 984
BsmAI GTCTC 2 cut(s) 345, 428
BsmFI GGGAC 1 cut(s) 806
BsnI GGCC 1 cut(s) 498
Bsp143I GATC 1 cut(s) 711
BspACI CCGC 3 cut(s) 301, 424, 724
BspANI GGCC 1 cut(s) 498
BspCNI CTCAG 2 cut(s) 18, 823
BspHI TCATGA 1 cut(s) 730
BspLI GGNNCC 5 cut(s) 258, 273, 674, 713, 953
BspPI GGATC 2 cut(s) 706, 719
BspT107I GGYRCC 2 cut(s) 256, 951
BsrDI GCAATG 1 cut(s) 50
BsrI ACTGG 4 cut(s) 313, 499, 676, 800
BssMI GATC 1 cut(s) 711
Bst2UI CCWGG 1 cut(s) 331
Bst4CI ACNGT 3 cut(s) 109, 163, 761
Bst6I CTCTTC 1 cut(s) 660
BstC8I GCNNGC 3 cut(s) 198, 298, 990
BstDEI CTNAG 2 cut(s) 26, 831
BstF5I GGATG 2 cut(s) 243, 477
BstH2I RGCGCY 1 cut(s) 724
BstHHI GCGC 2 cut(s) 336, 723
BstKTI GATC 1 cut(s) 714
BstMAI GTCTC 2 cut(s) 345, 428
BstMBI GATC 1 cut(s) 711
BstMWI GCNNNNNNNGC 2 cut(s) 41, 670
BstNI CCWGG 1 cut(s) 331
BstSCI CCNGG 1 cut(s) 329
BstV1I GCAGC 6 cut(s) 56, 502, 521, 676, 941, 944
BstV2I GAAGAC 2 cut(s) 135, 140
BstX2I RGATCY 1 cut(s) 711
BstXI CCANNNNNNTGG 1 cut(s) 683
BstYI RGATCY 1 cut(s) 711
BsuRI GGCC 1 cut(s) 498
BtsCI GGATG 2 cut(s) 243, 477
BtsI GCAGTG 1 cut(s) 462
BtsIMutI CAGTG 3 cut(s) 462, 683, 766
Cac8I GCNNGC 3 cut(s) 198, 298, 990
CciI TCATGA 1 cut(s) 730
CfoI GCGC 2 cut(s) 336, 723
Cfr13I GGNCC 1 cut(s) 271
Csp6I GTAC 1 cut(s) 952
CspCI CAANNNNNGTGG 2 cut(s) 249, 284
CviAII CATG 3 cut(s) 139, 731, 788
CviQI GTAC 1 cut(s) 952
DdeI CTNAG 2 cut(s) 26, 831
DpnI GATC 1 cut(s) 713
DpnII GATC 1 cut(s) 711
DraI TTTAAA 1 cut(s) 802
Eam1104I CTCTTC 1 cut(s) 660
EarI CTCTTC 1 cut(s) 660
Eco47I GGWCC 1 cut(s) 271
Eco57I CTGAAG 1 cut(s) 680
EcoRII CCWGG 1 cut(s) 329
EcoT22I ATGCAT 1 cut(s) 247
FaeI CATG 3 cut(s) 142, 734, 791
FaqI GGGAC 1 cut(s) 806
FatI CATG 3 cut(s) 138, 730, 787
Fnu4HI GCNGC 8 cut(s) 45, 301, 516, 535, 690, 724, 930, 933
FokI GGATG 2 cut(s) 250, 484
Fsp4HI GCNGC 8 cut(s) 45, 301, 516, 535, 690, 724, 930, 933
FspBI CTAG 2 cut(s) 468, 864
GlaI GCGC 2 cut(s) 335, 722
GluI GCNGC 8 cut(s) 45, 301, 516, 535, 690, 724, 930, 933
GsuI CTGGAG 3 cut(s) 330, 569, 659
HaeII RGCGCY 1 cut(s) 724
HaeIII GGCC 1 cut(s) 498
HhaI GCGC 2 cut(s) 336, 723
Hin1II CATG 3 cut(s) 142, 734, 791
Hin6I GCGC 2 cut(s) 334, 721
HinP1I GCGC 2 cut(s) 334, 721
HindIII AAGCTT 1 cut(s) 662
HinfI GANTC 4 cut(s) 156, 632, 781, 903
Hpy166II GTNNAC 5 cut(s) 210, 271, 317, 421, 764
Hpy188I TCNGA 2 cut(s) 492, 660
Hpy188III TCNNGA 2 cut(s) 548, 731
Hpy8I GTNNAC 5 cut(s) 210, 271, 317, 421, 764
HpyAV CCTTC 1 cut(s) 965
HpyCH4III ACNGT 3 cut(s) 109, 163, 761
HpyCH4IV ACGT 1 cut(s) 290
HpyCH4V TGCA 6 cut(s) 44, 245, 475, 518, 537, 929
HpyF10VI GCNNNNNNNGC 2 cut(s) 41, 670
HpyF3I CTNAG 2 cut(s) 26, 831
HpySE526I ACGT 1 cut(s) 290
Hsp92II CATG 3 cut(s) 142, 734, 791
HspAI GCGC 2 cut(s) 334, 721
KpnI GGTACC 1 cut(s) 955
Kzo9I GATC 1 cut(s) 711
LmnI GCTCC 3 cut(s) 464, 678, 872
Lsp1109I GCAGC 6 cut(s) 56, 502, 521, 676, 941, 944
LweI GCATC 1 cut(s) 462
MaeI CTAG 2 cut(s) 468, 864
MaeII ACGT 1 cut(s) 290
MaeIII GTNAC 4 cut(s) 226, 525, 891, 971
MalI GATC 1 cut(s) 713
MboI GATC 1 cut(s) 711
MboII GAAGA 7 cut(s) 135, 138, 145, 169, 616, 629, 647
MflI RGATCY 1 cut(s) 711
MluCI AATT 6 cut(s) 541, 584, 641, 735, 804, 1002
MmeI TCCRAC 1 cut(s) 689
MnlI CCTC 8 cut(s) 122, 177, 305, 509, 544, 795, 863, 890
Mph1103I ATGCAT 1 cut(s) 247
MseI TTAA 2 cut(s) 570, 801
MslI CAYNNNNRTG 2 cut(s) 189, 946
MspA1I CMGCKG 2 cut(s) 515, 726
MspR9I CCNGG 1 cut(s) 331
MvaI CCWGG 1 cut(s) 331
MwoI GCNNNNNNNGC 2 cut(s) 41, 670
NdeII GATC 1 cut(s) 711
NlaIII CATG 3 cut(s) 142, 734, 791
NlaIV GGNNCC 5 cut(s) 258, 273, 674, 713, 953
NmuCI GTSAC 1 cut(s) 525
NsiI ATGCAT 1 cut(s) 247
PagI TCATGA 1 cut(s) 730
PfeI GAWTC 4 cut(s) 156, 632, 781, 903
PflFI GACNNNGTC 1 cut(s) 139
PkrI GCNGC 8 cut(s) 46, 302, 517, 536, 691, 725, 931, 934
Psp6I CCWGG 1 cut(s) 329
PspGI CCWGG 1 cut(s) 329
PspN4I GGNNCC 5 cut(s) 258, 273, 674, 713, 953
PspPI GGNCC 1 cut(s) 271
PsuI RGATCY 1 cut(s) 711
PsyI GACNNNGTC 1 cut(s) 139
PvuII CAGCTG 1 cut(s) 515
RsaI GTAC 1 cut(s) 953
RsaNI GTAC 1 cut(s) 952
RseI CAYNNNNRTG 2 cut(s) 189, 946
SaqAI TTAA 2 cut(s) 570, 801
SatI GCNGC 8 cut(s) 45, 301, 516, 535, 690, 724, 930, 933
Sau3AI GATC 1 cut(s) 711
Sau96I GGNCC 1 cut(s) 271
ScrFI CCNGG 1 cut(s) 331
SfaNI GCATC 1 cut(s) 462
SinI GGWCC 1 cut(s) 271
SmiMI CAYNNNNRTG 2 cut(s) 189, 946
Sse9I AATT 6 cut(s) 541, 584, 641, 735, 804, 1002
SsiI CCGC 3 cut(s) 301, 424, 724
SspMI CTAG 2 cut(s) 468, 864
StyD4I CCNGG 1 cut(s) 329
TaaI ACNGT 3 cut(s) 109, 163, 761
TaiI ACGT 1 cut(s) 293
TasI AATT 6 cut(s) 541, 584, 641, 735, 804, 1002
TauI GCSGC 2 cut(s) 303, 726
TfiI GAWTC 4 cut(s) 156, 632, 781, 903
Tru1I TTAA 2 cut(s) 570, 801
Tru9I TTAA 2 cut(s) 570, 801
TscAI CASTG 3 cut(s) 469, 683, 766
TseFI GTSAC 1 cut(s) 525
TseI GCWGC 6 cut(s) 44, 515, 534, 689, 929, 932
Tsp45I GTSAC 1 cut(s) 525
TspDTI ATGAA 2 cut(s) 89, 747
TspGWI ACGGA 1 cut(s) 556
TspRI CASTG 3 cut(s) 469, 683, 766
Tth111I GACNNNGTC 1 cut(s) 139
VpaK11BI GGWCC 1 cut(s) 271
XapI RAATTY 3 cut(s) 584, 641, 735
XspI CTAG 2 cut(s) 468, 864
Zsp2I ATGCAT 1 cut(s) 247
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.