MD05G1164600.v1.1

Protein NRT1 PTR FAMILY

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0007992
Physical Location & Seq
Reverse (-)
29331808 .. 29332250
443 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1164600.v1.1.491

Sequence Viewer

Length: 381 bp
ATGTTCCAACCCCTTTGGAGGATCGGACTGGGGCTAACACTGGCTTCTGGGTCAATGTCTATAGCTGCCCTAGTTGAAGCTAAGAGGTGCGAAGCGGCTCACAATGATGTCATATTGCCTGTGTTTTGGCTAGGATGGCAATTCCTCTTGTTAGGAGTTTGTGACGTGCCTACTTTTGGAGGAATGTTCGAGTTCTTTTACTCAGACGCCAAGTACCATGAGGAGTATGTGCACTGCATTGTCATGGTGTTCAATATCTTTGGGAATGTCTTTGGAGGAGATGATTTGAATCTTTTGCGCTCGGATTTGTTTTGCACACTTCTTGCCATTCTTAGTACCGTCAATTTTCTTAATTACATGTATTGGGCTAAAAGGTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PTR2

Protein Analysis

127

Amino Acids

14.41

Weight (kDa)

5.12

Isoelectric Point (pI)

54.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PTR2 PF00854 2 - 94 2.2e-09 POT family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 95
AclWI GGATC 1 cut(s) 29
AcyI GRCGYC 1 cut(s) 207
AfaI GTAC 2 cut(s) 215, 337
AfiI CCNNNNNNNGG 2 cut(s) 18, 176
AflIII ACRYGT 1 cut(s) 357
AgsI TTSAA 3 cut(s) 77, 253, 289
AjiI CACGTC 1 cut(s) 166
AluBI AGCT 2 cut(s) 65, 80
AluI AGCT 2 cut(s) 65, 80
Alw21I GWGCWC 1 cut(s) 234
Alw44I GTGCAC 1 cut(s) 230
AlwI GGATC 1 cut(s) 29
ApaLI GTGCAC 1 cut(s) 230
ApeKI GCWGC 1 cut(s) 65
AspLEI GCGC 1 cut(s) 300
BaeGI GKGCMC 1 cut(s) 234
Bbv12I GWGCWC 1 cut(s) 234
BbvI GCAGC 1 cut(s) 52
BccI CCATC 1 cut(s) 129
BfaI CTAG 2 cut(s) 71, 131
BfmI CTRYAG 1 cut(s) 60
BisI GCNGC 2 cut(s) 66, 96
BlsI GCNGC 2 cut(s) 67, 97
BmgBI CACGTC 1 cut(s) 166
BmrI ACTGGG 1 cut(s) 38
BmuI ACTGGG 1 cut(s) 38
BsaBI GATNNNNATC 1 cut(s) 288
BsaHI GRCGYC 1 cut(s) 207
Bsc4I CCNNNNNNNGG 2 cut(s) 18, 176
Bse1I ACTGG 2 cut(s) 33, 45
Bse8I GATNNNNATC 1 cut(s) 288
BseGI GGATG 1 cut(s) 140
BseJI GATNNNNATC 1 cut(s) 288
BseLI CCNNNNNNNGG 2 cut(s) 18, 176
BseMII CTCAG 1 cut(s) 216
BseNI ACTGG 2 cut(s) 33, 45
BseRI GAGGAG 2 cut(s) 236, 291
BseSI GKGCMC 1 cut(s) 234
BseXI GCAGC 1 cut(s) 52
BsiHKAI GWGCWC 1 cut(s) 234
BslI CCNNNNNNNGG 2 cut(s) 18, 176
Bsp1286I GDGCHC 1 cut(s) 234
Bsp143I GATC 1 cut(s) 21
BspACI CCGC 1 cut(s) 95
BspCNI CTCAG 1 cut(s) 215
BspPI GGATC 1 cut(s) 29
BsrI ACTGG 2 cut(s) 33, 45
BssMI GATC 1 cut(s) 21
BssNI GRCGYC 1 cut(s) 207
Bst4CI ACNGT 1 cut(s) 340
BstACI GRCGYC 1 cut(s) 207
BstDEI CTNAG 3 cut(s) 81, 202, 332
BstF5I GGATG 1 cut(s) 140
BstHHI GCGC 1 cut(s) 300
BstKTI GATC 1 cut(s) 24
BstMBI GATC 1 cut(s) 21
BstMWI GCNNNNNNNGC 1 cut(s) 136
BstNSI RCATGY 1 cut(s) 361
BstSFI CTRYAG 1 cut(s) 60
BstSLI GKGCMC 1 cut(s) 234
BstV1I GCAGC 1 cut(s) 52
BtrI CACGTC 1 cut(s) 166
BtsCI GGATG 1 cut(s) 140
BtsI GCAGTG 1 cut(s) 232
BtsIMutI CAGTG 2 cut(s) 38, 232
CfoI GCGC 1 cut(s) 300
CseI GACGC 1 cut(s) 215
Csp6I GTAC 2 cut(s) 214, 336
CviAII CATG 3 cut(s) 218, 244, 358
CviJI RGCY 7 cut(s) 34, 44, 65, 80, 98, 130, 368
CviKI_1 RGCY 7 cut(s) 34, 44, 65, 80, 98, 130, 368
CviQI GTAC 2 cut(s) 214, 336
DdeI CTNAG 3 cut(s) 81, 202, 332
DpnI GATC 1 cut(s) 23
DpnII GATC 1 cut(s) 21
FaeI CATG 3 cut(s) 221, 247, 361
FaiI YATR 6 cut(s) 62, 113, 219, 228, 245, 359
FatI CATG 3 cut(s) 217, 243, 357
Fnu4HI GCNGC 2 cut(s) 66, 96
FokI GGATG 1 cut(s) 147
Fsp4HI GCNGC 2 cut(s) 66, 96
FspBI CTAG 2 cut(s) 71, 131
GlaI GCGC 1 cut(s) 299
GluI GCNGC 2 cut(s) 66, 96
HgaI GACGC 1 cut(s) 215
HhaI GCGC 1 cut(s) 300
Hin1I GRCGYC 1 cut(s) 207
Hin1II CATG 3 cut(s) 221, 247, 361
Hin6I GCGC 1 cut(s) 298
HinP1I GCGC 1 cut(s) 298
HinfI GANTC 1 cut(s) 289
Hpy166II GTNNAC 1 cut(s) 232
Hpy188I TCNGA 3 cut(s) 26, 205, 304
Hpy8I GTNNAC 1 cut(s) 232
HpyCH4III ACNGT 1 cut(s) 340
HpyCH4IV ACGT 1 cut(s) 165
HpyCH4V TGCA 3 cut(s) 232, 237, 315
HpyF10VI GCNNNNNNNGC 1 cut(s) 136
HpyF3I CTNAG 3 cut(s) 81, 202, 332
HpySE526I ACGT 1 cut(s) 165
Hsp92I GRCGYC 1 cut(s) 207
Hsp92II CATG 3 cut(s) 221, 247, 361
HspAI GCGC 1 cut(s) 298
Kzo9I GATC 1 cut(s) 21
LpnPI CCDG 4 cut(s) 14, 26, 33, 132
Lsp1109I GCAGC 1 cut(s) 52
MaeI CTAG 2 cut(s) 71, 131
MaeII ACGT 1 cut(s) 165
MaeIII GTNAC 1 cut(s) 161
MalI GATC 1 cut(s) 23
MboI GATC 1 cut(s) 21
MhlI GDGCHC 1 cut(s) 234
MluCI AATT 3 cut(s) 140, 343, 352
MmeI TCCRAC 1 cut(s) 31
MnlI CCTC 6 cut(s) 12, 78, 155, 173, 214, 269
MseI TTAA 1 cut(s) 351
MslI CAYNNNNRTG 2 cut(s) 105, 242
MwoI GCNNNNNNNGC 1 cut(s) 136
NdeII GATC 1 cut(s) 21
NlaIII CATG 3 cut(s) 221, 247, 361
NmuCI GTSAC 1 cut(s) 161
NspI RCATGY 1 cut(s) 361
PciI ACATGT 1 cut(s) 357
PfeI GAWTC 1 cut(s) 289
PkrI GCNGC 2 cut(s) 67, 97
PscI ACATGT 1 cut(s) 357
RsaI GTAC 2 cut(s) 215, 337
RsaNI GTAC 2 cut(s) 214, 336
RseI CAYNNNNRTG 2 cut(s) 105, 242
SaqAI TTAA 1 cut(s) 351
SatI GCNGC 2 cut(s) 66, 96
Sau3AI GATC 1 cut(s) 21
SduI GDGCHC 1 cut(s) 234
SetI ASST 5 cut(s) 67, 82, 89, 168, 377
SfcI CTRYAG 1 cut(s) 60
SmiMI CAYNNNNRTG 2 cut(s) 105, 242
Sse9I AATT 3 cut(s) 140, 343, 352
SsiI CCGC 1 cut(s) 95
SspMI CTAG 2 cut(s) 71, 131
TaaI ACNGT 1 cut(s) 340
TaiI ACGT 1 cut(s) 168
TaqI TCGA 1 cut(s) 189
TasI AATT 3 cut(s) 140, 343, 352
TauI GCSGC 1 cut(s) 98
TfiI GAWTC 1 cut(s) 289
Tru1I TTAA 1 cut(s) 351
Tru9I TTAA 1 cut(s) 351
TscAI CASTG 2 cut(s) 45, 239
TseFI GTSAC 1 cut(s) 161
TseI GCWGC 1 cut(s) 65
Tsp45I GTSAC 1 cut(s) 161
TspRI CASTG 2 cut(s) 45, 239
VneI GTGCAC 1 cut(s) 230
XceI RCATGY 1 cut(s) 361
XspI CTAG 2 cut(s) 71, 131
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.