MD10G1153900.v1.1

Protein NRT1 PTR FAMILY

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0007992
Physical Location & Seq
Reverse (-)
24150970 .. 24153718
2749 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1153900.v1.1.491

Sequence Viewer

Length: 1605 bp
ATGAGTATAGAAAGAGATGTCATTCAGAAAGAAAGTGATGATGACCGATATTTTGACTGGAAAGGAAGAGAGGCAGATCCTCGAAAGCACGGTGGAACCAGAGCTGCTGCCTTTGCATGTGTTGTGGAGGTGTTGACAAACATGGTGTTTTTAGCCAATGCCAGTAACTTTGTGAGATACTTCTTGTTGTCAATGCATTACCCACATGCTACAGCAGCAAATATGGTGACGAATTTTATTGGAACCTCCTTTTTGCTCTCCATCCTGGGAGGATTGATCAGTGACTCACTCTTCACAAGATTCACAACCTTAATCATCTCCTCTATCATAGAGTTGCTGGGATTAATTTTGCTGAGAATCCAAGCTCAGCACGCAAAATTTCGACCAGCATCGGGTGCTACGCCTTCTCGGTCTCAAGCAGCCATTCTATACACTGGCCTTTATGTCGTTGCTACCGGTGTAGGTGGAATCAATGCGTCCTTACCTGCACACGGTGCTGATCAGCTTGATCACTGCAATCAGAGACTCATCTCTACCTACTTCAATTGGTTTTTCTTTTCCCTGTGCCTTGGGGGCCTCCTAAGCTGTACTGTTATGGTTTGGGTGGAAGAGAACAATGGCTGGAATTCGAGCCTTACTATTACAATTATAGCCTTGAGTTTGGCACTTTCAATTTTCGTCAGTGGGAGCCCATTTTATCGACATAAACGTCCTGGAGGAAGCGCATTGACAAGAATCTTTAAGGTTCTTGCTTCTGCAGTTCGAAACTGGAAGGCCTCACCCGAAGAGAGAGTATTGGAGAACCAGTATGCAGTATCTGCAATCAATTCGAACGGAGTTAATGTGCGATCCCATAACAAGTATAAGTTCTTAGACAAAGCATTAATTGATAGCACAATCAGTGCAGCTCAAGTTAAGGAAACAAAAACTTTTATAGGCCTTCTACCCATCTTTGCAAGCACAATCATGATGAATTGCTGCCTAGCCCAACTTCAGACATACTCAGTGGTACAAGGGAGCACAATGAACCGAAAACTACACAATTTCGAAATCCCAGTTCAGTCATTAACCGTACTCCCCCTCTCCGTTATGCTTGCTTCTATTCCCTTATACGAACGATTCCGAAGAATCTTAGGAGACAAAAACTCAGCAAAAAGTCACATCTTCCAACCACTTTGGAGGATTGGAGTAGGGCTAGCACTAGCATCTGTATCAATGGCTGTAGCCACCATAGTCGAATCTCAGAGACGCAAGGTGGCTCATAAAAATGTTACATTGTCTTTCTTCTGGCTTGGCTGGCAATACCTCTTCCTTGGAGTCTCAGACATGCTCACTCTTGGAGGAATGCTCGAGTTCTTTTACTCGGAAGCGCCTGATAGCATGAGAAGCATGTCCACTTCATTGTCATGGTGCTCAACATCAATGGGTTACTTTTTGAGCTCAGTTTTGGTGTCCATAGCAAATTCATTGAGTGGAAGAATTGGTAAAGAGTGGCTTGGAGGAGCTGATGTGAATCATTCACGTTTGGATCTGTTTTACACACTTCTCTGCATTCTCAACACTCTCAATGTTCTTAATTACATATACTGGGCTAGGAAATATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PTR2

Protein Analysis

535

Amino Acids

59.31

Weight (kDa)

9.39

Isoelectric Point (pI)

38.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PTR2 PF00854 101 - 492 9.3e-82 POT family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 708
Acc36I ACCTGC 1 cut(s) 493
AclWI GGATC 3 cut(s) 71, 843, 1536
AcsI RAATTY 4 cut(s) 232, 377, 625, 1462
AcuI CTGAAG 1 cut(s) 977
AdeI CACNNNGTG 1 cut(s) 494
AfaI GTAC 3 cut(s) 589, 1011, 1074
AfiI CCNNNNNNNGG 2 cut(s) 392, 491
AgeI ACCGGT 1 cut(s) 455
AgsI TTSAA 2 cut(s) 544, 672
AjnI CCWGG 2 cut(s) 264, 712
AluBI AGCT 7 cut(s) 104, 365, 505, 585, 908, 1440, 1505
AluI AGCT 7 cut(s) 104, 365, 505, 585, 908, 1440, 1505
Alw21I GWGCWC 3 cut(s) 1022, 1415, 1442
Alw26I GTCTC 5 cut(s) 417, 517, 1131, 1240, 1324
AlwI GGATC 3 cut(s) 71, 843, 1536
AlwNI CAGNNNCTG 1 cut(s) 818
Ama87I CYCGRG 1 cut(s) 1349
AoxI GGCC 4 cut(s) 436, 574, 774, 937
ApeKI GCWGC 6 cut(s) 104, 107, 215, 419, 905, 978
ApoI RAATTY 4 cut(s) 232, 377, 625, 1462
ArsI GACNNNNNNTTYG 2 cut(s) 35, 67
AseI ATTAAT 2 cut(s) 344, 884
AsiGI ACCGGT 1 cut(s) 455
AspLEI GCGC 2 cut(s) 725, 1372
AspS9I GGNCC 1 cut(s) 574
AsuHPI GGTGA 2 cut(s) 238, 771
AsuII TTCGAA 3 cut(s) 763, 830, 1047
AsuNHI GCTAGC 1 cut(s) 1195
AvaI CYCGRG 1 cut(s) 1349
BanII GRGCYC 2 cut(s) 692, 1442
Bbv12I GWGCWC 3 cut(s) 1022, 1415, 1442
BbvI GCAGC 6 cut(s) 91, 94, 227, 431, 917, 965
BccI CCATC 2 cut(s) 269, 956
BcgI CGANNNNNNTGC 2 cut(s) 810, 844
BciT130I CCWGG 2 cut(s) 266, 714
BclI TGATCA 3 cut(s) 276, 499, 508
BcoDI GTCTC 5 cut(s) 417, 517, 1131, 1240, 1324
BfaI CTAG 4 cut(s) 983, 1196, 1202, 1593
BfmI CTRYAG 3 cut(s) 210, 756, 1221
BfoI RGCGCY 1 cut(s) 1373
BfuAI ACCTGC 1 cut(s) 493
BglI GCCNNNNNGGC 1 cut(s) 573
BisI GCNGC 6 cut(s) 105, 108, 216, 420, 906, 979
BlpI GCTNAGC 1 cut(s) 366
BlsI GCNGC 6 cut(s) 106, 109, 217, 421, 907, 980
Bme1390I CCNGG 2 cut(s) 266, 714
BmeT110I CYCGRG 1 cut(s) 1349
BmgT120I GGNCC 1 cut(s) 574
BmiI GGNNCC 4 cut(s) 97, 244, 575, 689
BmrFI CCNGG 2 cut(s) 266, 714
BmrI ACTGGG 2 cut(s) 1049, 1597
BmsI GCATC 2 cut(s) 398, 1214
BmtI GCTAGC 1 cut(s) 1199
BmuI ACTGGG 2 cut(s) 1049, 1597
BplI GAGNNNNNCTC 2 cut(s) 1332, 1364
BpmI CTGGAG 1 cut(s) 735
Bpu10I CCTNAGC 1 cut(s) 581
Bpu1102I GCTNAGC 1 cut(s) 366
Bpu14I TTCGAA 3 cut(s) 763, 830, 1047
BpuEI CTTGAG 3 cut(s) 399, 676, 894
BsaBI GATNNNNATC 1 cut(s) 1512
BsaI GGTCTC 1 cut(s) 417
BsaJI CCNNGG 3 cut(s) 265, 568, 1312
BsaWI WCCGGW 1 cut(s) 455
BsaXI ACNNNNNCTCC 6 cut(s) 791, 821, 828, 858, 1494, 1524
Bsc4I CCNNNNNNNGG 2 cut(s) 392, 491
Bse118I RCCGGY 1 cut(s) 455
Bse1I ACTGG 7 cut(s) 62, 162, 439, 773, 805, 1055, 1592
Bse8I GATNNNNATC 1 cut(s) 1512
BseBI CCWGG 2 cut(s) 266, 714
BseDI CCNNGG 3 cut(s) 265, 568, 1312
BseGI GGATG 1 cut(s) 261
BseJI GATNNNNATC 1 cut(s) 1512
BseLI CCNNNNNNNGG 2 cut(s) 392, 491
BseMII CTCAG 7 cut(s) 344, 380, 1017, 1161, 1256, 1335, 1455
BseNI ACTGG 7 cut(s) 62, 162, 439, 773, 805, 1055, 1592
BseRI GAGGAG 2 cut(s) 310, 1515
BseXI GCAGC 6 cut(s) 91, 94, 227, 431, 917, 965
BseYI CCCAGC 1 cut(s) 337
BsgI GTGCAG 2 cut(s) 471, 924
BshFI GGCC 4 cut(s) 438, 576, 776, 939
BshTI ACCGGT 1 cut(s) 455
BsiHKAI GWGCWC 3 cut(s) 1022, 1415, 1442
BsiHKCI CYCGRG 1 cut(s) 1349
BsiSI CCGG 1 cut(s) 456
BslI CCNNNNNNNGG 2 cut(s) 392, 491
BsmAI GTCTC 5 cut(s) 417, 517, 1131, 1240, 1324
BsmBI CGTCTC 1 cut(s) 1240
BsmI GAATGC 2 cut(s) 1350, 1551
BsnI GGCC 4 cut(s) 438, 576, 776, 939
Bso31I GGTCTC 1 cut(s) 417
BsoBI CYCGRG 1 cut(s) 1349
Bsp119I TTCGAA 3 cut(s) 763, 830, 1047
Bsp1286I GDGCHC 4 cut(s) 692, 1022, 1415, 1442
Bsp143I GATC 6 cut(s) 76, 276, 499, 508, 848, 1528
Bsp1720I GCTNAGC 1 cut(s) 366
BspANI GGCC 4 cut(s) 438, 576, 776, 939
BspCNI CTCAG 7 cut(s) 345, 379, 1016, 1160, 1255, 1334, 1454
BspHI TCATGA 1 cut(s) 966
BspLI GGNNCC 4 cut(s) 97, 244, 575, 689
BspMAI CTGCAG 1 cut(s) 760
BspMI ACCTGC 1 cut(s) 493
BspOI GCTAGC 1 cut(s) 1199
BspPI GGATC 3 cut(s) 71, 843, 1536
BspT104I TTCGAA 3 cut(s) 763, 830, 1047
BspTNI GGTCTC 1 cut(s) 417
BsrFI RCCGGY 1 cut(s) 455
BsrI ACTGG 7 cut(s) 62, 162, 439, 773, 805, 1055, 1592
BssAI RCCGGY 1 cut(s) 455
BssECI CCNNGG 3 cut(s) 265, 568, 1312
BssMI GATC 6 cut(s) 76, 276, 499, 508, 848, 1528
BssT1I CCWWGG 2 cut(s) 568, 1312
Bst2UI CCWGG 2 cut(s) 266, 714
Bst4CI ACNGT 4 cut(s) 92, 494, 592, 1072
Bst6I CTCTTC 5 cut(s) 61, 296, 603, 780, 1313
BstAPI GCANNNNNTGC 3 cut(s) 395, 494, 818
BstBI TTCGAA 3 cut(s) 763, 830, 1047
BstC8I GCNNGC 5 cut(s) 372, 958, 1095, 1197, 1298
BstF5I GGATG 1 cut(s) 261
BstH2I RGCGCY 1 cut(s) 1373
BstHHI GCGC 2 cut(s) 725, 1372
BstKTI GATC 6 cut(s) 79, 279, 502, 511, 851, 1531
BstMAI GTCTC 5 cut(s) 417, 517, 1131, 1240, 1324
BstMBI GATC 6 cut(s) 76, 276, 499, 508, 848, 1528
BstNI CCWGG 2 cut(s) 266, 714
BstNSI RCATGY 4 cut(s) 120, 209, 1330, 1393
BstSCI CCNGG 2 cut(s) 264, 712
BstSFI CTRYAG 3 cut(s) 210, 756, 1221
BstV1I GCAGC 6 cut(s) 91, 94, 227, 431, 917, 965
BstX2I RGATCY 2 cut(s) 76, 1528
BstYI RGATCY 2 cut(s) 76, 1528
BsuRI GGCC 4 cut(s) 438, 576, 776, 939
BtsCI GGATG 1 cut(s) 261
BtsI GCAGTG 1 cut(s) 511
BtsIMutI CAGTG 6 cut(s) 286, 432, 511, 688, 907, 1011
BveI ACCTGC 1 cut(s) 493
Cac8I GCNNGC 5 cut(s) 372, 958, 1095, 1197, 1298
CaiI CAGNNNCTG 1 cut(s) 818
CciI TCATGA 1 cut(s) 966
CfoI GCGC 2 cut(s) 725, 1372
Cfr10I RCCGGY 1 cut(s) 455
Cfr13I GGNCC 1 cut(s) 574
CseI GACGC 2 cut(s) 465, 1257
Csp6I GTAC 3 cut(s) 588, 1010, 1073
CspAI ACCGGT 1 cut(s) 455
CviAII CATG 8 cut(s) 117, 142, 206, 967, 1327, 1381, 1390, 1407
CviQI GTAC 3 cut(s) 588, 1010, 1073
DpnI GATC 6 cut(s) 78, 278, 501, 510, 850, 1530
DpnII GATC 6 cut(s) 76, 276, 499, 508, 848, 1528
DraIII CACNNNGTG 1 cut(s) 494
DrdI GACNNNNNNGTC 1 cut(s) 708
DseDI GACNNNNNNGTC 1 cut(s) 708
Eam1104I CTCTTC 5 cut(s) 61, 296, 603, 780, 1313
EarI CTCTTC 5 cut(s) 61, 296, 603, 780, 1313
Ecl136II GAGCTC 1 cut(s) 1440
Eco130I CCWWGG 2 cut(s) 568, 1312
Eco147I AGGCCT 2 cut(s) 776, 939
Eco24I GRGCYC 2 cut(s) 692, 1442
Eco31I GGTCTC 1 cut(s) 417
Eco53kI GAGCTC 1 cut(s) 1440
Eco57I CTGAAG 1 cut(s) 977
Eco88I CYCGRG 1 cut(s) 1349
EcoICRI GAGCTC 1 cut(s) 1440
EcoO109I RGGNCCY 1 cut(s) 574
EcoRI GAATTC 1 cut(s) 625
EcoRII CCWGG 2 cut(s) 264, 712
EcoT14I CCWWGG 2 cut(s) 568, 1312
EcoT22I ATGCAT 1 cut(s) 198
EcoT38I GRGCYC 2 cut(s) 692, 1442
ErhI CCWWGG 2 cut(s) 568, 1312
Esp3I CGTCTC 1 cut(s) 1240
FaeI CATG 8 cut(s) 120, 145, 209, 970, 1330, 1384, 1393, 1410
FalI AAGNNNNNCTT 2 cut(s) 1479, 1511
FatI CATG 8 cut(s) 116, 141, 205, 966, 1326, 1380, 1389, 1406
FbaI TGATCA 3 cut(s) 276, 499, 508
Fnu4HI GCNGC 6 cut(s) 105, 108, 216, 420, 906, 979
FokI GGATG 1 cut(s) 248
FriOI GRGCYC 2 cut(s) 692, 1442
Fsp4HI GCNGC 6 cut(s) 105, 108, 216, 420, 906, 979
FspBI CTAG 4 cut(s) 983, 1196, 1202, 1593
GlaI GCGC 2 cut(s) 724, 1371
GluI GCNGC 6 cut(s) 105, 108, 216, 420, 906, 979
GsaI CCCAGC 1 cut(s) 341
GsuI CTGGAG 1 cut(s) 735
HaeII RGCGCY 1 cut(s) 1373
HaeIII GGCC 4 cut(s) 438, 576, 776, 939
HapII CCGG 1 cut(s) 456
HgaI GACGC 2 cut(s) 465, 1257
HhaI GCGC 2 cut(s) 725, 1372
Hin1II CATG 8 cut(s) 120, 145, 209, 970, 1330, 1384, 1393, 1410
Hin6I GCGC 2 cut(s) 723, 1370
HinP1I GCGC 2 cut(s) 723, 1370
HincII GTYRAC 1 cut(s) 135
HindII GTYRAC 1 cut(s) 135
HpaII CCGG 1 cut(s) 456
HphI GGTGA 2 cut(s) 238, 771
Hpy166II GTNNAC 2 cut(s) 135, 1395
Hpy188I TCNGA 7 cut(s) 27, 522, 996, 1124, 1245, 1324, 1366
Hpy188III TCNNGA 1 cut(s) 967
Hpy8I GTNNAC 2 cut(s) 135, 1395
HpyAV CCTTC 3 cut(s) 414, 766, 950
HpyCH4III ACNGT 4 cut(s) 92, 494, 592, 1072
HpyCH4IV ACGT 2 cut(s) 709, 1522
HpySE526I ACGT 2 cut(s) 709, 1522
Hsp92II CATG 8 cut(s) 120, 145, 209, 970, 1330, 1384, 1393, 1410
HspAI GCGC 2 cut(s) 723, 1370
Ksp22I TGATCA 3 cut(s) 276, 499, 508
Kzo9I GATC 6 cut(s) 76, 276, 499, 508, 848, 1528
LmnI GCTCC 3 cut(s) 687, 1017, 1502
Lsp1109I GCAGC 6 cut(s) 91, 94, 227, 431, 917, 965
LweI GCATC 2 cut(s) 398, 1214
MaeI CTAG 4 cut(s) 983, 1196, 1202, 1593
MaeII ACGT 2 cut(s) 709, 1522
MaeIII GTNAC 6 cut(s) 164, 226, 281, 1157, 1270, 1427
MalI GATC 6 cut(s) 78, 278, 501, 510, 850, 1530
MboI GATC 6 cut(s) 76, 276, 499, 508, 848, 1528
MboII GAAGA 9 cut(s) 78, 283, 620, 797, 1137, 1156, 1276, 1300, 1488
MfeI CAATTG 1 cut(s) 544
MflI RGATCY 2 cut(s) 76, 1528
MhlI GDGCHC 4 cut(s) 692, 1022, 1415, 1442
MlyI GAGTC 3 cut(s) 278, 519, 1326
MmeI TCCRAC 1 cut(s) 1192
Mph1103I ATGCAT 1 cut(s) 198
MseI TTAA 8 cut(s) 311, 344, 741, 840, 884, 915, 1067, 1575
MslI CAYNNNNRTG 3 cut(s) 965, 1266, 1405
MspI CCGG 1 cut(s) 456
MspR9I CCNGG 2 cut(s) 266, 714
MunI CAATTG 1 cut(s) 544
Mva1269I GAATGC 2 cut(s) 1350, 1551
MvaI CCWGG 2 cut(s) 266, 714
NdeII GATC 6 cut(s) 76, 276, 499, 508, 848, 1528
NheI GCTAGC 1 cut(s) 1195
NlaIII CATG 8 cut(s) 120, 145, 209, 970, 1330, 1384, 1393, 1410
NlaIV GGNNCC 4 cut(s) 97, 244, 575, 689
NmuCI GTSAC 3 cut(s) 226, 281, 1157
NsiI ATGCAT 1 cut(s) 198
NspI RCATGY 4 cut(s) 120, 209, 1330, 1393
NspV TTCGAA 3 cut(s) 763, 830, 1047
PaeR7I CTCGAG 1 cut(s) 1349
PagI TCATGA 1 cut(s) 966
PceI AGGCCT 2 cut(s) 776, 939
PcsI WCGNNNNNNNCGW 1 cut(s) 706
PctI GAATGC 2 cut(s) 1350, 1551
PfeI GAWTC 8 cut(s) 300, 357, 468, 735, 1119, 1128, 1238, 1513
PfoI TCCNGGA 1 cut(s) 712
PinAI ACCGGT 1 cut(s) 455
PkrI GCNGC 6 cut(s) 106, 109, 217, 421, 907, 980
PleI GAGTC 3 cut(s) 278, 519, 1325
PpsI GAGTC 3 cut(s) 278, 519, 1325
PshBI ATTAAT 2 cut(s) 344, 884
Psp124BI GAGCTC 1 cut(s) 1442
Psp6I CCWGG 2 cut(s) 264, 712
PspFI CCCAGC 1 cut(s) 337
PspGI CCWGG 2 cut(s) 264, 712
PspN4I GGNNCC 4 cut(s) 97, 244, 575, 689
PspPI GGNCC 1 cut(s) 574
PspXI VCTCGAGB 1 cut(s) 1349
PstI CTGCAG 1 cut(s) 760
PstNI CAGNNNCTG 1 cut(s) 818
PsuI RGATCY 2 cut(s) 76, 1528
RsaI GTAC 3 cut(s) 589, 1011, 1074
RsaNI GTAC 3 cut(s) 588, 1010, 1073
RseI CAYNNNNRTG 3 cut(s) 965, 1266, 1405
SacI GAGCTC 1 cut(s) 1442
SaqAI TTAA 8 cut(s) 311, 344, 741, 840, 884, 915, 1067, 1575
SatI GCNGC 6 cut(s) 105, 108, 216, 420, 906, 979
Sau3AI GATC 6 cut(s) 76, 276, 499, 508, 848, 1528
Sau96I GGNCC 1 cut(s) 574
SchI GAGTC 3 cut(s) 278, 519, 1326
ScrFI CCNGG 2 cut(s) 266, 714
SduI GDGCHC 4 cut(s) 692, 1022, 1415, 1442
SfaNI GCATC 2 cut(s) 398, 1214
SfcI CTRYAG 3 cut(s) 210, 756, 1221
Sfr274I CTCGAG 1 cut(s) 1349
SfuI TTCGAA 3 cut(s) 763, 830, 1047
SlaI CTCGAG 1 cut(s) 1349
SmiMI CAYNNNNRTG 3 cut(s) 965, 1266, 1405
SmlI CTYRAG 4 cut(s) 414, 655, 909, 1349
SmoI CTYRAG 4 cut(s) 414, 655, 909, 1349
SseBI AGGCCT 2 cut(s) 776, 939
SspI AATATT 1 cut(s) 1601
SspMI CTAG 4 cut(s) 983, 1196, 1202, 1593
SstI GAGCTC 1 cut(s) 1442
StuI AGGCCT 2 cut(s) 776, 939
StyD4I CCNGG 2 cut(s) 264, 712
StyI CCWWGG 2 cut(s) 568, 1312
TaaI ACNGT 4 cut(s) 92, 494, 592, 1072
TaiI ACGT 2 cut(s) 712, 1525
TaqI TCGA 9 cut(s) 82, 382, 629, 700, 763, 830, 1047, 1236, 1350
TaqII GACCGA 2 cut(s) 60, 399
TatI WGTACW 1 cut(s) 587
TfiI GAWTC 8 cut(s) 300, 357, 468, 735, 1119, 1128, 1238, 1513
Tru1I TTAA 8 cut(s) 311, 344, 741, 840, 884, 915, 1067, 1575
Tru9I TTAA 8 cut(s) 311, 344, 741, 840, 884, 915, 1067, 1575
TscAI CASTG 6 cut(s) 286, 439, 518, 688, 907, 1011
TseFI GTSAC 3 cut(s) 226, 281, 1157
TseI GCWGC 6 cut(s) 104, 107, 215, 419, 905, 978
Tsp45I GTSAC 3 cut(s) 226, 281, 1157
TspDTI ATGAA 4 cut(s) 986, 1040, 1389, 1455
TspGWI ACGGA 2 cut(s) 849, 1075
TspRI CASTG 6 cut(s) 286, 439, 518, 688, 907, 1011
VspI ATTAAT 2 cut(s) 344, 884
XapI RAATTY 4 cut(s) 232, 377, 625, 1462
XceI RCATGY 4 cut(s) 120, 209, 1330, 1393
XhoI CTCGAG 1 cut(s) 1349
XspI CTAG 4 cut(s) 983, 1196, 1202, 1593
Zsp2I ATGCAT 1 cut(s) 198
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.