Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0003906

Physical Location & Seq

Genomic Coordinates Forward (+)

32211857 .. 32217145

Gene Structure 5289 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1194600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3624 bp

ATGGAGGAAGACGAAGATGCAATTTTGCTCAACAGTTTGAAAGGGGCGACGGCGGCGAATCCCGAGGATATCGAGAGGGACATTCTATCAGGGGTACATAACAATGGCAATGGTAGTAATGTTAGTGAAGTTGGAGGGAGTAATGAGGAGGAGGAACGCCTTGAGAAATCCGAAAGTATTGATCCATCGGTTGCCAGTGGGGTCAAATTGTATAGCAAATTGAGGGCAGTCGAGTTTGAAATTGATGCCGTTGAGTCCACTGTTGAACCAGAACAAACGCGGAGTGAAGAGGGTGATCGTGATGGTGGTGATGGTGCAGAGGCGGGAAATAAGGCAGATAACCTCCAGCATGCCCTGGCTACTGACCGGTTAAGAAGTCTAAAGAAAACAAAGGCGCAACTTGAGAAGGAGCTTTCGGATTTAGGCAAGGGAAGACCGTCCAAGGGTATTGCGCGTGACAAAGTCTTATTGGATATTGTCAAGGACAAGCCTGCACCCAAGAGGAAGTCGAAACAAGTTCAGATATCCGGAAAAAAGCTAGAGAAAAGGATCAAAACGGTCTCATTTGATGAGGATGATGACTTTGATGCTGTGTTGGATGCAGCATCTGCAGGCTTTGTTGAAACGGAAAGGGATGAATTAGTTAGGAAAGGAATTTTAACTCCTTTCCATAAGCTAAAGGGCTTTGAACGCGGCCTTCAAGAACAAGGGCCATCTCGGGGGCGTAATATTCCTGCAGAAGGAGGCAGGAGTGATGATTTTGTTTCAGCCAGTGTTGCTAGAGCAGTGCAGTCACTTTCAGATGCTGCTCAAGCTCGCCCAGCTACCAAGTTGCTTGATCCAGAAGCTCTACCAAAGCTTGATCCACCCACTCATCCTTTTAAGAGGCTAAGGAAACCGCTTAAAATTCCTCAATCCCTAGAAAATGGTACACAGAGGAATAAAAGTTCAGGAAAGAAAAGGAAGCGTCCTTTGCCTGACAAAAGATGGAGAAAGCGCATTTCTCTTGAGGAAAATAATTTGCATGAAAGTGAAAACACTCCCAGCTGTGAAGAAGAAAACCAAGATGATGTTGGAGATGTAGATGATGATGAGTATCCGCATGTGACCCTAGAAGGGGGGCTGAAAATTCCGGAGTACATTTTTAAACAACTCTTTGACTATCAAAAGGTAGGGGTCCAGTGGCTATGGGAACTGCATTGCCAAAGGGCGGGCGGAATTATTGGAGATGAGATGGGTCTTGGTAAAACCATCCAGGTTCTGTCTTTCCTCGGTGCATTGCATTTCAGTCATATGTATAAACCAAGCATTATTGTCTGCCCAGTTACTCTTTTGCGCCAATGGAAAAGGGAGGCTAAAAAATGGTACCCGAGCTTTCATGTGGAGTTACTTCATGATTCAGCTCGGGAGCCTGCTATTAAAAAAAAAGGATCAAAGTCTGATGAAAGTGATTCTGATAGTGAAGGTTCATCGGACAGTGATTATGAGAGACCAGAATCATCTAAAAGCACCAAAAAATGGGATTCCTTGATCAACCGTGTTTTGAGGTCGGAATCTGGGTTGCTAATTACAACTTATGAGCAACTCCGTATTGTAGGGGAAAGTTTGCTCGACGTTGATTGGGGTTATGCAGTTCTGGATGAAGGACACCGAATCAGGAACCCAAATGCAGAAGTTACTCTAGTTTGTAAGCAGTTACAAACAGTACATCGTATTATAATGACAGGTGCACCAATTCAGAACAAGCTGACTGAACTGTGGTCCTTGTTTGATTTTGTTTTCCCTGGAAAGTTGGGGGTGTTGCCCATATTTGAGGCTGAGTTTGCAGTTCCCATATCTGTTGGTGGCTATGCGAATGCTTCACCATTACAAGTATCCACTGCATACAGGTGTGCTGTGGTTTTGCGAGACTTGATCATGCCGTATCTCCTCCGGCGCATGAAGGCTGATGTGGATGCGCAGCTGACTAAGAAGACTGAACATGTCATCTTTTGCAGCCTCACTGATGAGCAAAGATCTGCATATAGAGCATTTCTTGCTAGTTCTGAGGTGGAACAGATTATGGATGGTAATAGGAATTCGCTTTATGGAATTGATGTCATGCGCAAGATCTGCAACCACCCTGATCTGCTTGAGAGGGAACACTGCGGCCAGAATCCAGATTATGGGAATCTAGAACGCAGTGGGAAATTAAAAGTTGTTTCACAAGTGCTTAAAGTTTGGAAGGATCAAGGGCATCGTGTTCTTCTTTTTACACAAACTCAGCAAATGCTTGACATTATTGAAAGTTATCTGGTTTCTGATGGTTATAGTTACCGGAGGATGGATGGTCTTACTCCCATCAAACAGAGGATGGCCTTAATAGATGAATTTAACAACTCAAGTGATGTGTTTGTTTTCATTTTAACAACTAAAGTGGGTGGTTTAGGAACAAATCTGACAGGTGCAAACAGGGTGATCATCTTTGATCCCGACTGGAACCCTTCAACTGACATGCAGGCAAGAGAGCGGGCTTGGCGTATTGGTCAAAAGCGGGATGTAACCATATATAGATTGATAACCCGTGGAACCATAGAGGAGAAGGTATATCATAGACAAATTTACAAGCATTTCCTGACCAATAAGATATTGAAGAACCCACAACAGAGAAGGTTCTTTAAAGCTAGAGACATGAAGGATCTTTTCACTCTGAGTGATGACAGAGAGAATGGGTCTACCGAAACAGCTCATCTGTTCGGTCAATTATCTGAAGATGCCAACGTCATTGGTGTGCAGAATGAACCCGATACGCAATATTCTCAGAGAGGTTCTGTACAGTCTACCAATGGTGTTGTGGCTGACAATGCAAATAACTCAGAGGCTGGACCATCCAGGAGGAATGGGAAAGAAAAGGCTGACCAGAGTGACGGTGAAGTAGATGAAGAGACAAATATTTTGAGGTGCCTTTTTGACGCCCAAGGGATACATAGTGCTATGAACCATGATGTAATCATGAATGCCCATGATGAAGAGAAGATGAAGCTTGACGAGCAAGCTTCCCGAGTTGCACAAAGAGCAGCAGAAGCGTTGCGCCAGTCACGGATGCTTCGAAGCCGTGATGGCGTGTCTGTCCCCACATGGACTGGGAAATCAGGAATGGCAGGTGCACCATCAGCTGTCCGTGGGAAATTCGGGTCTACTCTGAACTCCCAGTTGGTCAAGAATACCAAACGGTCAGATGAAGGCCCCAGCAACGGCACAAACGGTTTCGTGGCTGGGGCTCCTGCTGGGAAGGCATTATCTTCTGCTGAATTACTGGCAAAAATTAGAGGAAACCAAGAAAGAGCTGTGGAAGCAGGAATTGAGCATCAGTTTAACCGAGCAAAATCCTTTGATGTTGGACCTTCTAGGACCGGCCACAACTTAGGCGGAGTGCAACCCGAAGTAGTGATTCGTCAGATCTGTACATTCATCCAACAAAGAGGCGGAAGTACGGATTCTTCAAGCATAGTGCAGCATTTTAAGGACAGAATACCATCAAGGGATCTTCCCTTGTTTAAGAATCTTTTGAAGGAAATAGCCAAACTGGAGAAAAACCAAAACGGAGCAGTTTGGGTTCTAAAGCCAGAGTTTGATCGTCAGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001207 GO:0001208 GO:0003674 GO:0003824 GO:0006139 GO:0006259 GO:0006281 GO:0006283 GO:0006289 GO:0006325 GO:0006355 GO:0006357 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006996 GO:0008094 GO:0008150 GO:0008152 GO:0009314 GO:0009411 GO:0009416 GO:0009628 GO:0009889 GO:0009987 GO:0010212 GO:0010332 GO:0010468 GO:0010556 GO:0010767 GO:0016043 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0033554 GO:0034641 GO:0034644 GO:0034728 GO:0042623 GO:0043170 GO:0043618 GO:0043620 GO:0043933 GO:0044237 GO:0044238 GO:0044260 GO:0046483 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051252 GO:0051276 GO:0051716 GO:0060255 GO:0065007 GO:0071214 GO:0071478 GO:0071482 GO:0071704 GO:0071824 GO:0071840 GO:0080090 GO:0090304 GO:0104004 GO:1901360 GO:1903506 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

ko03420 map03420

Pfam Domains

Protein Families

Helicase_C SNF2_N

Protein Analysis

1208

Amino Acids

134.87

Weight (kDa)

6.49

Isoelectric Point (pI)

40.34

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
SNF2-rel_dom	PF00176	388 - 710	9.4e-77	SNF2-related domain
HDA2-3	PF11496	699 - 861	8.5e-07	Class II histone deacetylase complex subunits 2 and 3
Helicase_C	PF00271	730 - 842	3.7e-25	Helicase conserved C-terminal domain
WHD_Rad26_CSB	PF25875	1142 - 1204	4.8e-14	Rad26/CSB winged helix DNA-binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0003906)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G18760 AT2G18760 AT2G18760 AT2G18760 AT2G18760 AT2G18760 AT2G18760
fragaria_vesca	FvH4_2g21200 FvH4_2g21200 FvH4_2g21200 FvH4_2g21200 FvH4_2g21200 FvH4_2g21200
malus_domestica	MD05G1194600.v1.1
prunus_persica	Prupe.8G221300_v2.0.a1
pyrus_communis	pycom05g18190 pycom10g15720
rosa_chinensis	RchiOBHm_Chr6g0287701
rosa_laevigata	RLG00000012472
rosa_multiflora	Rmu_sc0003335.1_g000002 Rmu_sc0037270.1_g000001 Rmu_sc0037271.1_g000001
rosa_roxburghii	Rroxscaffold_7G00181080 Rroxscaffold_7G00181270
rosa_rugosa	Rorug06G0190800 Rorug06G0190900 Rorug06G0191000 Rorug06G0191100
rosa_samantha	Rh6AG301300 Rh6BG307000 Rh6CG302500 Rh6CG315200 Rh6DG298700
rosa_wichuraiana	Rw6G026000

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1718
AarI	CACCTGC	1 cut(s)	3131
Acc16I	TGCGCA	2 cut(s)	1959, 2105
Acc36I	ACCTGC	1 cut(s)	3131
Acc65I	GGTACC	1 cut(s)	1365
AccB1I	GGYRCC	2 cut(s)	1365, 2940
AccB7I	CCANNNNNTGG	2 cut(s)	1518, 2165
AccBSI	CCGCTC	1 cut(s)	2509
AccI	GTMKAC	3 cut(s)	2714, 2819, 3176
AccII	CGCG	3 cut(s)	280, 454, 693
AccIII	TCCGGA	2 cut(s)	527, 1132
AclWI	GGATC	9 cut(s)	176, 557, 833, 857, 1438, 2235, 2462, 2685, 3531
AcoI	YGGCCR	2 cut(s)	2149, 3394
AcsI	RAATTY	7 cut(s)	654, 906, 1128, 2077, 2369, 2598, 3167
AcuI	CTGAAG	1 cut(s)	2769
AcyI	GRCGYC	1 cut(s)	2952
AfaI	GTAC	8 cut(s)	96, 931, 1139, 1367, 1707, 2814, 3445, 3472
AflIII	ACRYGT	1 cut(s)	1981
AgeI	ACCGGT	1 cut(s)	366
AjnI	CCWGG	4 cut(s)	354, 1254, 1783, 2870
AjuI	GAANNNNNNNTTGG	2 cut(s)	3176, 3208
AloI	GAACNNNNNNTCC	2 cut(s)	931, 963
Alw21I	GWGCWC	2 cut(s)	1732, 3148
Alw26I	GTCTC	5 cut(s)	565, 1483, 1902, 2661, 2918
Alw44I	GTGCAC	2 cut(s)	1728, 3144
AlwI	GGATC	9 cut(s)	176, 557, 833, 857, 1438, 2235, 2462, 2685, 3531
AlwNI	CAGNNNCTG	4 cut(s)	608, 806, 1261, 2861
Ama87I	CYCGRG	5 cut(s)	62, 717, 1369, 1404, 3039
Aor13HI	TCCGGA	2 cut(s)	527, 1132
AoxI	GGCC	6 cut(s)	694, 710, 2149, 2355, 3223, 3394
ApaLI	GTGCAC	2 cut(s)	1728, 3144
ApeKI	GCWGC	6 cut(s)	602, 806, 1960, 1995, 3056, 3493
ApoI	RAATTY	7 cut(s)	654, 906, 1128, 2077, 2369, 2598, 3167
ArsI	GACNNNNNNTTYG	2 cut(s)	2917, 2949
AsiGI	ACCGGT	1 cut(s)	366
Asp718I	GGTACC	1 cut(s)	1365
AspLEI	GCGC	8 cut(s)	397, 454, 999, 1338, 1938, 1960, 2106, 3072
AspS9I	GGNCC	7 cut(s)	710, 1177, 1761, 2864, 3224, 3380, 3390
AsuHPI	GGTGA	5 cut(s)	305, 320, 1854, 2467, 2921
AsuII	TTCGAA	1 cut(s)	3088
AvaI	CYCGRG	5 cut(s)	62, 717, 1369, 1404, 3039
AvaII	GGWCC	5 cut(s)	1177, 1761, 2864, 3380, 3390
BaeGI	GKGCMC	2 cut(s)	1732, 3148
BanI	GGYRCC	2 cut(s)	1365, 2940
BanII	GRGCYC	1 cut(s)	3262
BarI	GAAGNNNNNNTAC	2 cut(s)	3463, 3495
BbsI	GAAGAC	3 cut(s)	15, 439, 1979
Bbv12I	GWGCWC	2 cut(s)	1732, 3148
BbvI	GCAGC	6 cut(s)	614, 793, 1972, 2007, 3068, 3505
BceAI	ACGGC	5 cut(s)	66, 233, 1906, 3078, 3250
BciT130I	CCWGG	4 cut(s)	356, 1256, 1785, 2872
BciVI	GTATCC	3 cut(s)	1107, 1885, 2955
BclI	TGATCA	3 cut(s)	1530, 1914, 2457
BcoDI	GTCTC	5 cut(s)	565, 1483, 1902, 2661, 2918
BfaI	CTAG	9 cut(s)	539, 780, 920, 1112, 1682, 2040, 2174, 2664, 3387
BfmI	CTRYAG	2 cut(s)	609, 735
BfuAI	ACCTGC	1 cut(s)	3131
BfuI	GTATCC	3 cut(s)	1107, 1885, 2955
BglI	GCCNNNNNGGC	1 cut(s)	3099
BglII	AGATCT	3 cut(s)	2015, 2109, 3438
BisI	GCNGC	9 cut(s)	54, 603, 694, 807, 1961, 1996, 2149, 3057, 3494
BlsI	GCNGC	9 cut(s)	55, 604, 695, 808, 1962, 1997, 2150, 3058, 3495
Bme1390I	CCNGG	4 cut(s)	356, 1256, 1785, 2872
Bme18I	GGWCC	5 cut(s)	1177, 1761, 2864, 3380, 3390
BmeT110I	CYCGRG	5 cut(s)	62, 717, 1369, 1404, 3039
BmgT120I	GGNCC	7 cut(s)	710, 1177, 1761, 2864, 3224, 3380, 3390
BmiI	GGNNCC	9 cut(s)	1178, 1367, 1410, 1661, 2480, 2569, 2942, 3226, 3261
BmrFI	CCNGG	4 cut(s)	356, 1256, 1785, 2872
BmrI	ACTGGG	3 cut(s)	1316, 3132, 3184
BmuI	ACTGGG	3 cut(s)	1316, 3132, 3184
BpiI	GAAGAC	3 cut(s)	15, 439, 1979
BpmI	CTGGAG	2 cut(s)	329, 3587
Bpu10I	CCTNAGC	1 cut(s)	890
Bpu14I	TTCGAA	1 cut(s)	3088
BpuEI	CTTGAG	6 cut(s)	182, 422, 795, 1028, 2153, 2365
BsaBI	GATNNNNATC	1 cut(s)	1095
BsaHI	GRCGYC	1 cut(s)	2952
BsaI	GGTCTC	2 cut(s)	565, 1483
BsaJI	CCNNGG	8 cut(s)	63, 354, 441, 1270, 1783, 2563, 2956, 3160
BsaWI	WCCGGW	4 cut(s)	366, 527, 1132, 2316
Bse118I	RCCGGY	2 cut(s)	366, 3392
Bse3DI	GCAATG	3 cut(s)	115, 1198, 1277
Bse8I	GATNNNNATC	1 cut(s)	1095
BseAI	TCCGGA	2 cut(s)	527, 1132
BseBI	CCWGG	4 cut(s)	356, 1256, 1785, 2872
BseDI	CCNNGG	8 cut(s)	63, 354, 441, 1270, 1783, 2563, 2956, 3160
BseJI	GATNNNNATC	1 cut(s)	1095
BseMI	GCAATG	3 cut(s)	115, 1198, 1277
BseMII	CTCAG	6 cut(s)	1809, 2037, 2276, 2681, 2813, 2868
BseRI	GAGGAG	4 cut(s)	161, 164, 1919, 2591
BseSI	GKGCMC	2 cut(s)	1732, 3148
BseXI	GCAGC	6 cut(s)	614, 793, 1972, 2007, 3068, 3505
BseYI	CCCAGC	5 cut(s)	820, 1043, 3227, 3254, 3266
BsgI	GTGCAG	5 cut(s)	336, 477, 809, 2792, 3512
Bsh1236I	CGCG	3 cut(s)	280, 454, 693
BshFI	GGCC	6 cut(s)	696, 712, 2151, 2357, 3225, 3396
BshNI	GGYRCC	2 cut(s)	1365, 2940
BshTI	ACCGGT	1 cut(s)	366
BsiHKAI	GWGCWC	2 cut(s)	1732, 3148
BsiHKCI	CYCGRG	5 cut(s)	62, 717, 1369, 1404, 3039
BsiSI	CCGG	6 cut(s)	367, 528, 1133, 1933, 2317, 3393
BslFI	GGGAC	2 cut(s)	92, 3095
BsmAI	GTCTC	5 cut(s)	565, 1483, 1902, 2661, 2918
BsmFI	GGGAC	2 cut(s)	92, 3095
BsmI	GAATGC	2 cut(s)	1861, 3001
BsnI	GGCC	6 cut(s)	696, 712, 2151, 2357, 3225, 3396
Bso31I	GGTCTC	2 cut(s)	565, 1483
BsoBI	CYCGRG	5 cut(s)	62, 717, 1369, 1404, 3039
Bsp119I	TTCGAA	1 cut(s)	3088
Bsp1286I	GDGCHC	3 cut(s)	1732, 3148, 3262
Bsp13I	TCCGGA	2 cut(s)	527, 1132
Bsp1407I	TGTACA	2 cut(s)	2812, 3443
BspANI	GGCC	6 cut(s)	696, 712, 2151, 2357, 3225, 3396
BspCNI	CTCAG	6 cut(s)	1810, 2038, 2275, 2682, 2812, 2867
BspEI	TCCGGA	2 cut(s)	527, 1132
BspFNI	CGCG	3 cut(s)	280, 454, 693
BspHI	TCATGA	2 cut(s)	1393, 2991
BspLI	GGNNCC	9 cut(s)	1178, 1367, 1410, 1661, 2480, 2569, 2942, 3226, 3261
BspMAI	CTGCAG	2 cut(s)	613, 739
BspMI	ACCTGC	1 cut(s)	3131
BspPI	GGATC	9 cut(s)	176, 557, 833, 857, 1438, 2235, 2462, 2685, 3531
BspT104I	TTCGAA	1 cut(s)	3088
BspT107I	GGYRCC	2 cut(s)	1365, 2940
BspTNI	GGTCTC	2 cut(s)	565, 1483
BsrBI	CCGCTC	1 cut(s)	2509
BsrDI	GCAATG	3 cut(s)	115, 1198, 1277
BsrFI	RCCGGY	2 cut(s)	366, 3392
BsrGI	TGTACA	2 cut(s)	2812, 3443
BssAI	RCCGGY	2 cut(s)	366, 3392
BssECI	CCNNGG	8 cut(s)	63, 354, 441, 1270, 1783, 2563, 2956, 3160
BssNI	GRCGYC	1 cut(s)	2952
BssT1I	CCWWGG	2 cut(s)	441, 2956
Bst2UI	CCWGG	4 cut(s)	356, 1256, 1785, 2872
Bst6I	CTCTTC	3 cut(s)	282, 2916, 3003
BstACI	GRCGYC	1 cut(s)	2952
BstAPI	GCANNNNNTGC	3 cut(s)	608, 2036, 2112
BstAUI	TGTACA	2 cut(s)	2812, 3443
BstBI	TTCGAA	1 cut(s)	3088
BstC8I	GCNNGC	9 cut(s)	351, 492, 613, 817, 1213, 1413, 2499, 2511, 3033
BstDEI	CTNAG	9 cut(s)	890, 1818, 1968, 2046, 2262, 2690, 2799, 2854, 3403
BstDSI	CCRYGG	2 cut(s)	2563, 3160
BstENI	CCTNNNNNAGG	1 cut(s)	738
BstFNI	CGCG	3 cut(s)	280, 454, 693
BstHHI	GCGC	8 cut(s)	397, 454, 999, 1338, 1938, 1960, 2106, 3072
BstMAI	GTCTC	5 cut(s)	565, 1483, 1902, 2661, 2918
BstNI	CCWGG	4 cut(s)	356, 1256, 1785, 2872
BstNSI	RCATGY	4 cut(s)	353, 1106, 1985, 2497
BstSCI	CCNGG	4 cut(s)	354, 1254, 1783, 2870
BstSFI	CTRYAG	2 cut(s)	609, 735
BstSLI	GKGCMC	2 cut(s)	1732, 3148
BstUI	CGCG	3 cut(s)	280, 454, 693
BstV1I	GCAGC	6 cut(s)	614, 793, 1972, 2007, 3068, 3505
BstV2I	GAAGAC	3 cut(s)	15, 439, 1979
BstX2I	RGATCY	5 cut(s)	2015, 2109, 2677, 3438, 3523
BstYI	RGATCY	5 cut(s)	2015, 2109, 2677, 3438, 3523
BsuI	GTATCC	3 cut(s)	1107, 1885, 2955
BsuRI	GGCC	6 cut(s)	696, 712, 2151, 2357, 3225, 3396
BtgI	CCRYGG	2 cut(s)	2563, 3160
BtsI	GCAGTG	4 cut(s)	792, 1878, 2143, 2188
BveI	ACCTGC	1 cut(s)	3131
Cac8I	GCNNGC	9 cut(s)	351, 492, 613, 817, 1213, 1413, 2499, 2511, 3033
CaiI	CAGNNNCTG	4 cut(s)	608, 806, 1261, 2861
CciI	TCATGA	2 cut(s)	1393, 2991
CfoI	GCGC	8 cut(s)	397, 454, 999, 1338, 1938, 1960, 2106, 3072
Cfr10I	RCCGGY	2 cut(s)	366, 3392
Cfr13I	GGNCC	7 cut(s)	710, 1177, 1761, 2864, 3224, 3380, 3390
CseI	GACGC	2 cut(s)	956, 2960
Csp6I	GTAC	8 cut(s)	95, 930, 1138, 1366, 1706, 2813, 3444, 3471
CspAI	ACCGGT	1 cut(s)	366
CspCI	CAANNNNNGTGG	2 cut(s)	2397, 2432
CviQI	GTAC	8 cut(s)	95, 930, 1138, 1366, 1706, 2813, 3444, 3471
DdeI	CTNAG	9 cut(s)	890, 1818, 1968, 2046, 2262, 2690, 2799, 2854, 3403
DraI	TTTAAA	2 cut(s)	1147, 2659
EaeI	YGGCCR	2 cut(s)	2149, 3394
Eam1104I	CTCTTC	3 cut(s)	282, 2916, 3003
EarI	CTCTTC	3 cut(s)	282, 2916, 3003
EciI	GGCGGA	3 cut(s)	1230, 3423, 3480
Eco130I	CCWWGG	2 cut(s)	441, 2956
Eco24I	GRGCYC	1 cut(s)	3262
Eco31I	GGTCTC	2 cut(s)	565, 1483
Eco32I	GATATC	2 cut(s)	70, 525
Eco47I	GGWCC	5 cut(s)	1177, 1761, 2864, 3380, 3390
Eco57I	CTGAAG	1 cut(s)	2769
Eco88I	CYCGRG	5 cut(s)	62, 717, 1369, 1404, 3039
EcoNI	CCTNNNNNAGG	1 cut(s)	738
EcoO109I	RGGNCCY	1 cut(s)	3224
EcoRI	GAATTC	1 cut(s)	2077
EcoRII	CCWGG	4 cut(s)	354, 1254, 1783, 2870
EcoRV	GATATC	2 cut(s)	70, 525
EcoT14I	CCWWGG	2 cut(s)	441, 2956
EcoT38I	GRGCYC	1 cut(s)	3262
ErhI	CCWWGG	2 cut(s)	441, 2956
FaqI	GGGAC	2 cut(s)	92, 3095
FauI	CCCGC	4 cut(s)	316, 1204, 2502, 2526
FauNDI	CATATG	1 cut(s)	1293
FbaI	TGATCA	3 cut(s)	1530, 1914, 2457
FblI	GTMKAC	3 cut(s)	2714, 2819, 3176
Fnu4HI	GCNGC	9 cut(s)	54, 603, 694, 807, 1961, 1996, 2149, 3057, 3494
FriOI	GRGCYC	1 cut(s)	3262
Fsp4HI	GCNGC	9 cut(s)	54, 603, 694, 807, 1961, 1996, 2149, 3057, 3494
FspBI	CTAG	9 cut(s)	539, 780, 920, 1112, 1682, 2040, 2174, 2664, 3387
FspI	TGCGCA	2 cut(s)	1959, 2105
GlaI	GCGC	8 cut(s)	396, 453, 998, 1337, 1937, 1959, 2105, 3071
GluI	GCNGC	9 cut(s)	54, 603, 694, 807, 1961, 1996, 2149, 3057, 3494
GsaI	CCCAGC	5 cut(s)	824, 1047, 3231, 3258, 3270
GsuI	CTGGAG	2 cut(s)	329, 3587
HaeIII	GGCC	6 cut(s)	696, 712, 2151, 2357, 3225, 3396
HapII	CCGG	6 cut(s)	367, 528, 1133, 1933, 2317, 3393
HgaI	GACGC	2 cut(s)	956, 2960
HhaI	GCGC	8 cut(s)	397, 454, 999, 1338, 1938, 1960, 2106, 3072
Hin1I	GRCGYC	1 cut(s)	2952
Hin6I	GCGC	8 cut(s)	395, 452, 997, 1336, 1936, 1958, 2104, 3070
HinP1I	GCGC	8 cut(s)	395, 452, 997, 1336, 1936, 1958, 2104, 3070
HindIII	AAGCTT	3 cut(s)	857, 3020, 3033
HpaII	CCGG	6 cut(s)	367, 528, 1133, 1933, 2317, 3393
HphI	GGTGA	5 cut(s)	305, 320, 1854, 2467, 2921
Hpy166II	GTNNAC	7 cut(s)	258, 932, 1730, 2715, 2820, 3146, 3177
Hpy8I	GTNNAC	7 cut(s)	258, 932, 1730, 2715, 2820, 3146, 3177
Hpy99I	CGWCG	2 cut(s)	52, 1616
HpyCH4IV	ACGT	2 cut(s)	1614, 2760
HpyF3I	CTNAG	9 cut(s)	890, 1818, 1968, 2046, 2262, 2690, 2799, 2854, 3403
HpySE526I	ACGT	2 cut(s)	1614, 2760
Hsp92I	GRCGYC	1 cut(s)	2952
HspAI	GCGC	8 cut(s)	395, 452, 997, 1336, 1936, 1958, 2104, 3070
Kpn2I	TCCGGA	2 cut(s)	527, 1132
KpnI	GGTACC	1 cut(s)	1369
Ksp22I	TGATCA	3 cut(s)	1530, 1914, 2457
LmnI	GCTCC	4 cut(s)	409, 1408, 3265, 3584
Lsp1109I	GCAGC	6 cut(s)	614, 793, 1972, 2007, 3068, 3505
MaeI	CTAG	9 cut(s)	539, 780, 920, 1112, 1682, 2040, 2174, 2664, 3387
MaeII	ACGT	2 cut(s)	1614, 2760
MbiI	CCGCTC	1 cut(s)	2509
MflI	RGATCY	5 cut(s)	2015, 2109, 2677, 3438, 3523
MhlI	GDGCHC	3 cut(s)	1732, 3148, 3262
MlyI	GAGTC	1 cut(s)	263
MmeI	TCCRAC	6 cut(s)	112, 576, 1054, 1530, 3358, 3478
MroI	TCCGGA	2 cut(s)	527, 1132
MslI	CAYNNNNRTG	4 cut(s)	102, 1029, 1888, 2768
MspA1I	CMGCKG	3 cut(s)	1047, 1963, 3155
MspI	CCGG	6 cut(s)	367, 528, 1133, 1933, 2317, 3393
MspR9I	CCNGG	4 cut(s)	356, 1256, 1785, 2872
Mva1269I	GAATGC	2 cut(s)	1861, 3001
MvaI	CCWGG	4 cut(s)	356, 1256, 1785, 2872
MvnI	CGCG	3 cut(s)	280, 454, 693
NdeI	CATATG	1 cut(s)	1293
NlaIV	GGNNCC	9 cut(s)	1178, 1367, 1410, 1661, 2480, 2569, 2942, 3226, 3261
NmuCI	GTSAC	5 cut(s)	455, 792, 1105, 2903, 3075
NsbI	TGCGCA	2 cut(s)	1959, 2105
NspI	RCATGY	4 cut(s)	353, 1106, 1985, 2497
NspV	TTCGAA	1 cut(s)	3088
PaeI	GCATGC	1 cut(s)	353
PagI	TCATGA	2 cut(s)	1393, 2991
PaqCI	CACCTGC	1 cut(s)	3131
PciI	ACATGT	1 cut(s)	1981
PcsI	WCGNNNNNNNCGW	1 cut(s)	3085
PctI	GAATGC	2 cut(s)	1861, 3001
PflFI	GACNNNGTC	1 cut(s)	461
PflMI	CCANNNNNTGG	2 cut(s)	1518, 2165
PfoI	TCCNGGA	1 cut(s)	2870
PinAI	ACCGGT	1 cut(s)	366
PkrI	GCNGC	9 cut(s)	55, 604, 695, 808, 1962, 1997, 2150, 3058, 3495
PleI	GAGTC	1 cut(s)	262
PpsI	GAGTC	1 cut(s)	262
PscI	ACATGT	1 cut(s)	1981
PsiI	TTATAA	1 cut(s)	1718
Psp6I	CCWGG	4 cut(s)	354, 1254, 1783, 2870
PspFI	CCCAGC	5 cut(s)	820, 1043, 3227, 3254, 3266
PspGI	CCWGG	4 cut(s)	354, 1254, 1783, 2870
PspN4I	GGNNCC	9 cut(s)	1178, 1367, 1410, 1661, 2480, 2569, 2942, 3226, 3261
PspPI	GGNCC	7 cut(s)	710, 1177, 1761, 2864, 3224, 3380, 3390
PstI	CTGCAG	2 cut(s)	613, 739
PstNI	CAGNNNCTG	4 cut(s)	608, 806, 1261, 2861
PsuI	RGATCY	5 cut(s)	2015, 2109, 2677, 3438, 3523
PsyI	GACNNNGTC	1 cut(s)	461
PvuII	CAGCTG	3 cut(s)	1047, 1963, 3155
RsaI	GTAC	8 cut(s)	96, 931, 1139, 1367, 1707, 2814, 3445, 3472
RsaNI	GTAC	8 cut(s)	95, 930, 1138, 1366, 1706, 2813, 3444, 3471
RseI	CAYNNNNRTG	4 cut(s)	102, 1029, 1888, 2768
SatI	GCNGC	9 cut(s)	54, 603, 694, 807, 1961, 1996, 2149, 3057, 3494
Sau96I	GGNCC	7 cut(s)	710, 1177, 1761, 2864, 3224, 3380, 3390
SchI	GAGTC	1 cut(s)	263
ScrFI	CCNGG	4 cut(s)	356, 1256, 1785, 2872
SduI	GDGCHC	3 cut(s)	1732, 3148, 3262
SfcI	CTRYAG	2 cut(s)	609, 735
SfuI	TTCGAA	1 cut(s)	3088
SinI	GGWCC	5 cut(s)	1177, 1761, 2864, 3380, 3390
SmiMI	CAYNNNNRTG	4 cut(s)	102, 1029, 1888, 2768
SmlI	CTYRAG	6 cut(s)	161, 401, 810, 1007, 2132, 2380
SmoI	CTYRAG	6 cut(s)	161, 401, 810, 1007, 2132, 2380
SphI	GCATGC	1 cut(s)	353
SspI	AATATT	3 cut(s)	730, 2795, 2932
SspMI	CTAG	9 cut(s)	539, 780, 920, 1112, 1682, 2040, 2174, 2664, 3387
StyD4I	CCNGG	4 cut(s)	354, 1254, 1783, 2870
StyI	CCWWGG	2 cut(s)	441, 2956
TaiI	ACGT	2 cut(s)	1617, 2763
TaqI	TCGA	5 cut(s)	72, 231, 509, 1611, 3088
TaqII	GACCGA	1 cut(s)	2726
TatI	WGTACW	4 cut(s)	1137, 1705, 2812, 3443
TauI	GCSGC	3 cut(s)	56, 696, 2151
TseFI	GTSAC	5 cut(s)	455, 792, 1105, 2903, 3075
TseI	GCWGC	6 cut(s)	602, 806, 1960, 1995, 3056, 3493
Tsp45I	GTSAC	5 cut(s)	455, 792, 1105, 2903, 3075
TspGWI	ACGGA	6 cut(s)	641, 1577, 3094, 3149, 3488, 3597
Tth111I	GACNNNGTC	1 cut(s)	461
Van91I	CCANNNNNTGG	2 cut(s)	1518, 2165
VneI	GTGCAC	2 cut(s)	1728, 3144
VpaK11BI	GGWCC	5 cut(s)	1177, 1761, 2864, 3380, 3390
XagI	CCTNNNNNAGG	1 cut(s)	738
XapI	RAATTY	7 cut(s)	654, 906, 1128, 2077, 2369, 2598, 3167
XbaI	TCTAGA	1 cut(s)	2173
XceI	RCATGY	4 cut(s)	353, 1106, 1985, 2497
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1070, 2830
XmiI	GTMKAC	3 cut(s)	2714, 2819, 3176
XspI	CTAG	9 cut(s)	539, 780, 920, 1112, 1682, 2040, 2174, 2664, 3387

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.