Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0012096

Physical Location & Seq

Genomic Coordinates Forward (+)

32776298 .. 32780166

Gene Structure 3869 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1200600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1656 bp

ATGGAATTCGCTAAGCGTTTCTCCAAGCGGCTCTTCCACAGCTTGTATTTTACGCAAAGCTGTGTGAAGAGGGAGGAATTTTACCAGCGGTTTGTATACAGCTACGTGAATCGGTTTCAATCGCAGCACTCGAAGGCAGAGAAAGGAGGAGAATCGGACACATCGAAAAAGCTGAAAGCTGTTGTTCCTCCGCAAGCTGCTGATGGCATTAGCAGCTATTCGAGTGAGGATGAAGGTTTGGTGGATGGTAAGGAGAAAAGGTTGGAGCTTGCTTGGCTGCCCAGAGTTCTCGAGCCAGCTCTTCAATTGTGCAGGTGGGCTCTGCCAACCCCAGCAGGAAATGGAGTGGGAAACAAAACCCCATCCACAACTCGATCAGTCTCAGAGATCATTGCAAACATCCAACGGAGTAAGATAGGAATTGAGGATTGGAGTTTGAGTGATCTCACCATTGGTTTGTTTCTTATTTATCTTCGTCAAGCTTCTGTAAATCCGTTTGAGGATGTTAATGGTGTTCAGGTCTCCTCAGATTCAATGGTGCAAGATCTCATCTACCACTCTGAGTTAGCGAAAGGTTCTTATAAGGATAATACTGCAGCTCTTGCAAGGAACACCATGCTTCGTGAAAGCAATGTCTTGAAATTTGTCAAAGATTCCAGTGTCATGAGGCCTGGATATTATATAGGGATAGATCCTCGTAAAAAACTTGTGATTTTAGGAATCCGTGGTACTCATACTGTATATGACCTTATAACTGATGTTGTTTCTTCAAGCGATGATGAAGTCACGTTTGAGGGTTATTCAACCCATTTTGGCACTGCGGAAGCTGCTCGCTGGTTTCTTAGTCACGAGATGGGAAATATAAGGAAGTGCTTGGAGAAATATAAGGGATTTAAGTTAAGACTAGTTGGTCATTCTCTCGGAGGTTCTACAGCTGCTTTACTTGCAATAATGCTCCGTAAAATGTCAGTTAAGGAGCTGGGGTTCAACCCAGAGATTGTCTCTGCCGTGGGGTATGCAACGCCACCTTGTGTCTCCAAAGAACTTGCTGAAAGCTGCTCCAGTTATGTCACAACTGTTGTGATGCAGGATGATATTATACCTAGGCTAAGTCCAGCATCTCTAACGAGGTTGAGGAGTGAAATTCTTAAAACCGATTGGATGAGTGTGATTGAGAAGGAAGATTGGAAAAGTGTCATAGATTTGGTTACAAATGCAAAGCAGGTTGTGTCTTCGGTGCAAGATGTTGCTCGGAAGCTTTCTGATTATGCAAAGTTTGGGAGCAGTAAACATTCTTCTGATGGTCCCATCATAAAGAAAATTCCTACTCCTCCCGGGGTCCCTTTAAATGAAAGAGAAGTAACAGAAAATGCTGGTGCTATTAAAAGTGGAGGAGCTGCCTGTAAAGTGCCTGAGGAATTATTTGTACCAGGTACCGTCTACTGCCTAAAGAGGAATGTGGATGCTCGTACCAGCAGCGGCGGCAGCAGAAGGGAGTATTTTACACTTTGGAAGAGGCATCCGGGTGAGCATTTTCAGAGGATTGTGCTTTCAAGCAACATAATAACTGACCACAAGTGTGTGAGCCATTACTATGCATTAAGAGATGTTCTCAAAGGTTTGCCGGCCTCCGATGATGAAGACGAATTTTCTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001505 GO:0001676 GO:0003674 GO:0003824 GO:0005575 GO:0005623 GO:0005886 GO:0006082 GO:0006629 GO:0006631 GO:0006638 GO:0006639 GO:0006690 GO:0008150 GO:0008152 GO:0009056 GO:0009058 GO:0009987 GO:0016020 GO:0016042 GO:0016298 GO:0016787 GO:0016788 GO:0019369 GO:0019752 GO:0032787 GO:0033559 GO:0042133 GO:0042136 GO:0043436 GO:0044237 GO:0044238 GO:0044242 GO:0044248 GO:0044249 GO:0044255 GO:0044281 GO:0044464 GO:0046339 GO:0046340 GO:0046461 GO:0046464 GO:0046486 GO:0046503 GO:0047372 GO:0052689 GO:0065007 GO:0065008 GO:0071704 GO:0071944 GO:1901568 GO:1901575

KEGG Pathways

Metabolic & Signaling

ko04723 ko04745 ko04925 map04723 map04745 map04925

Pfam Domains

Protein Families

Lipase_3

Protein Analysis

552

Amino Acids

61.31

Weight (kDa)

8.65

Isoelectric Point (pI)

49.97

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Lipase_3	PF01764	239 - 373	1.4e-27	Lipase (class 3)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0012096)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G42450
fragaria_vesca	FvH4_2g21920 FvH4_2g21920
malus_domestica	MD05G1200600.v1.1 MD10G1187200.v1.1
prunus_persica	Prupe.8G227200_v2.0.a1
pyrus_communis	pycom05g18740
rosa_chinensis	RchiOBHm_Chr6g0288821
rosa_laevigata	RLG00000012365
rosa_multiflora	Rmu_sc0005314.1_g000019
rosa_roxburghii	Rroxscaffold_7G00179870
rosa_rugosa	Rorug06G0198300 Rorug06G0198300 Rorug06G0198300
rosa_samantha	Rh6AG309600 Rh6BG315500 Rh6CG323000 Rh6DG308700
rosa_wichuraiana	Rw6G026760

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	582, 752
AarI	CACCTGC	1 cut(s)	303
AasI	GACNNNNNNGTC	1 cut(s)	909
Acc36I	ACCTGC	2 cut(s)	303, 1213
Acc65I	GGTACC	1 cut(s)	1433
AccB1I	GGYRCC	1 cut(s)	1433
AccI	GTMKAC	2 cut(s)	96, 1440
AciI	CCGC	6 cut(s)	28, 88, 191, 821, 1479, 1482
AclWI	GGATC	1 cut(s)	686
AcsI	RAATTY	6 cut(s)	5, 77, 641, 1143, 1320, 1646
AdeI	CACNNNGTG	1 cut(s)	1031
AfaI	GTAC	4 cut(s)	730, 1428, 1435, 1471
AgsI	TTSAA	8 cut(s)	119, 305, 534, 640, 771, 804, 988, 1554
AhlI	ACTAGT	1 cut(s)	904
AjnI	CCWGG	2 cut(s)	670, 1429
AjuI	GAANNNNNNNTTGG	4 cut(s)	17, 49, 412, 444
AleI	CACNNNNGTG	1 cut(s)	1578
AloI	GAACNNNNNNTCC	2 cut(s)	968, 1000
Alw26I	GTCTC	4 cut(s)	385, 526, 1006, 1039
AlwI	GGATC	1 cut(s)	686
Ama87I	CYCGRG	2 cut(s)	290, 1334
AoxI	GGCC	2 cut(s)	668, 1626
ApoI	RAATTY	6 cut(s)	5, 77, 641, 1143, 1320, 1646
Asp718I	GGTACC	1 cut(s)	1433
AspA2I	CCTAGG	1 cut(s)	1103
AspS9I	GGNCC	2 cut(s)	1304, 1339
AsuC2I	CCSGG	3 cut(s)	1335, 1336, 1524
AsuHPI	GGTGA	2 cut(s)	439, 1538
AvaI	CYCGRG	2 cut(s)	290, 1334
AvaII	GGWCC	2 cut(s)	1304, 1339
AvrII	CCTAGG	1 cut(s)	1103
AxyI	CCTNAGG	1 cut(s)	1413
BanI	GGYRCC	1 cut(s)	1433
BanII	GRGCYC	1 cut(s)	322
BarI	GAAGNNNNNNTAC	2 cut(s)	1279, 1311
BauI	CACGAG	1 cut(s)	848
BbsI	GAAGAC	2 cut(s)	1224, 1647
BccI	CCATC	6 cut(s)	197, 239, 370, 847, 1295, 1316
BceAI	ACGGC	1 cut(s)	992
BciT130I	CCWGG	2 cut(s)	672, 1431
BcnI	CCSGG	3 cut(s)	1335, 1336, 1524
BcoDI	GTCTC	4 cut(s)	385, 526, 1006, 1039
BcuI	ACTAGT	1 cut(s)	904
BfaI	CTAG	2 cut(s)	905, 1104
BfmI	CTRYAG	2 cut(s)	594, 930
BfuAI	ACCTGC	2 cut(s)	303, 1213
BglII	AGATCT	1 cut(s)	544
BlnI	CCTAGG	1 cut(s)	1103
BlpI	GCTNAGC	1 cut(s)	12
Bme1390I	CCNGG	5 cut(s)	672, 1335, 1336, 1431, 1524
Bme18I	GGWCC	2 cut(s)	1304, 1339
BmeT110I	CYCGRG	2 cut(s)	290, 1334
BmgT120I	GGNCC	2 cut(s)	1304, 1339
BmiI	GGNNCC	4 cut(s)	1306, 1340, 1341, 1435
BmrFI	CCNGG	5 cut(s)	672, 1335, 1336, 1431, 1524
BmsI	GCATC	4 cut(s)	1074, 1127, 1453, 1528
BpiI	GAAGAC	2 cut(s)	1224, 1647
BplI	GAGNNNNNCTC	4 cut(s)	986, 1018, 1596, 1628
BpmI	CTGGAG	1 cut(s)	1045
Bpu1102I	GCTNAGC	1 cut(s)	12
BpuMI	CCSGG	3 cut(s)	1335, 1336, 1524
BsaAI	YACGTR	1 cut(s)	106
BsaI	GGTCTC	1 cut(s)	526
BsaJI	CCNNGG	5 cut(s)	724, 1008, 1103, 1334, 1335
BsaXI	ACNNNNNCTCC	6 cut(s)	245, 275, 424, 454, 968, 998
Bse118I	RCCGGY	1 cut(s)	1624
Bse1I	ACTGG	2 cut(s)	657, 1062
Bse21I	CCTNAGG	1 cut(s)	1413
Bse3DI	GCAATG	2 cut(s)	390, 637
BseBI	CCWGG	2 cut(s)	672, 1431
BseDI	CCNNGG	5 cut(s)	724, 1008, 1103, 1334, 1335
BseGI	GGATG	9 cut(s)	235, 250, 362, 399, 508, 1096, 1167, 1468, 1519
BseMI	GCAATG	2 cut(s)	390, 637
BseMII	CTCAG	4 cut(s)	396, 540, 552, 1404
BseNI	ACTGG	2 cut(s)	657, 1062
BseRI	GAGGAG	5 cut(s)	162, 514, 1150, 1320, 1407
BseYI	CCCAGC	2 cut(s)	331, 979
BsgI	GTGCAG	1 cut(s)	331
BshFI	GGCC	2 cut(s)	670, 1628
BshNI	GGYRCC	1 cut(s)	1433
BsiHKCI	CYCGRG	2 cut(s)	290, 1334
BsiSI	CCGG	3 cut(s)	1335, 1523, 1625
BslFI	GGGAC	2 cut(s)	1290, 1325
BsmAI	GTCTC	4 cut(s)	385, 526, 1006, 1039
BsmFI	GGGAC	2 cut(s)	1290, 1325
BsnI	GGCC	2 cut(s)	670, 1628
Bso31I	GGTCTC	1 cut(s)	526
BsoBI	CYCGRG	2 cut(s)	290, 1334
Bsp1286I	GDGCHC	1 cut(s)	322
Bsp143I	GATC	5 cut(s)	374, 387, 442, 544, 691
Bsp1720I	GCTNAGC	1 cut(s)	12
BspACI	CCGC	6 cut(s)	28, 88, 191, 821, 1479, 1482
BspANI	GGCC	2 cut(s)	670, 1628
BspCNI	CTCAG	4 cut(s)	395, 539, 553, 1405
BspHI	TCATGA	1 cut(s)	663
BspLI	GGNNCC	4 cut(s)	1306, 1340, 1341, 1435
BspMAI	CTGCAG	1 cut(s)	598
BspMI	ACCTGC	2 cut(s)	303, 1213
BspPI	GGATC	1 cut(s)	686
BspQI	GCTCTTC	2 cut(s)	38, 306
BspT107I	GGYRCC	1 cut(s)	1433
BspTNI	GGTCTC	1 cut(s)	526
BsrDI	GCAATG	2 cut(s)	390, 637
BsrFI	RCCGGY	1 cut(s)	1624
BsrI	ACTGG	2 cut(s)	657, 1062
BssAI	RCCGGY	1 cut(s)	1624
BssECI	CCNNGG	5 cut(s)	724, 1008, 1103, 1334, 1335
BssMI	GATC	5 cut(s)	374, 387, 442, 544, 691
BssNAI	GTATAC	1 cut(s)	97
BssSI	CACGAG	1 cut(s)	848
BssT1I	CCWWGG	1 cut(s)	1103
Bst1107I	GTATAC	1 cut(s)	97
Bst2BI	CACGAG	1 cut(s)	848
Bst2UI	CCWGG	2 cut(s)	672, 1431
Bst4CI	ACNGT	3 cut(s)	739, 1078, 1438
Bst6I	CTCTTC	4 cut(s)	38, 62, 306, 1508
BstAPI	GCANNNNNTGC	1 cut(s)	602
BstBAI	YACGTR	1 cut(s)	106
BstC8I	GCNNGC	5 cut(s)	195, 270, 297, 832, 1626
BstDEI	CTNAG	7 cut(s)	12, 382, 526, 561, 842, 1109, 1413
BstDSI	CCRYGG	2 cut(s)	724, 1008
BstF5I	GGATG	9 cut(s)	235, 250, 362, 399, 508, 1096, 1167, 1468, 1519
BstKTI	GATC	5 cut(s)	377, 390, 445, 547, 694
BstMAI	GTCTC	4 cut(s)	385, 526, 1006, 1039
BstMBI	GATC	5 cut(s)	374, 387, 442, 544, 691
BstMWI	GCNNNNNNNGC	7 cut(s)	213, 274, 602, 827, 944, 1482, 1485
BstNI	CCWGG	2 cut(s)	672, 1431
BstSCI	CCNGG	5 cut(s)	670, 1333, 1334, 1429, 1522
BstSFI	CTRYAG	2 cut(s)	594, 930
BstV2I	GAAGAC	2 cut(s)	1224, 1647
BstX2I	RGATCY	2 cut(s)	544, 691
BstYI	RGATCY	2 cut(s)	544, 691
BstZ17I	GTATAC	1 cut(s)	97
Bsu36I	CCTNAGG	1 cut(s)	1413
BsuRI	GGCC	2 cut(s)	670, 1628
BtgI	CCRYGG	2 cut(s)	724, 1008
BtgZI	GCGATG	1 cut(s)	789
BtsCI	GGATG	9 cut(s)	235, 250, 362, 399, 508, 1096, 1167, 1468, 1519
BtsI	GCAGTG	1 cut(s)	816
BtsIMutI	CAGTG	2 cut(s)	664, 816
BveI	ACCTGC	2 cut(s)	303, 1213
Cac8I	GCNNGC	5 cut(s)	195, 270, 297, 832, 1626
CciI	TCATGA	1 cut(s)	663
Cfr10I	RCCGGY	1 cut(s)	1624
Cfr13I	GGNCC	2 cut(s)	1304, 1339
Cfr9I	CCCGGG	1 cut(s)	1334
CsiI	ACCWGGT	1 cut(s)	1429
Csp6I	GTAC	4 cut(s)	729, 1427, 1434, 1470
CviAII	CATG	2 cut(s)	616, 664
CviQI	GTAC	4 cut(s)	729, 1427, 1434, 1470
DdeI	CTNAG	7 cut(s)	12, 382, 526, 561, 842, 1109, 1413
DpnI	GATC	5 cut(s)	376, 389, 444, 546, 693
DpnII	GATC	5 cut(s)	374, 387, 442, 544, 691
DraI	TTTAAA	1 cut(s)	1347
DraIII	CACNNNGTG	1 cut(s)	1031
DrdI	GACNNNNNNGTC	1 cut(s)	909
DseDI	GACNNNNNNGTC	1 cut(s)	909
Eam1104I	CTCTTC	4 cut(s)	38, 62, 306, 1508
EarI	CTCTTC	4 cut(s)	38, 62, 306, 1508
Eco130I	CCWWGG	1 cut(s)	1103
Eco147I	AGGCCT	1 cut(s)	670
Eco24I	GRGCYC	1 cut(s)	322
Eco31I	GGTCTC	1 cut(s)	526
Eco47I	GGWCC	2 cut(s)	1304, 1339
Eco81I	CCTNAGG	1 cut(s)	1413
Eco88I	CYCGRG	2 cut(s)	290, 1334
EcoO109I	RGGNCCY	1 cut(s)	1339
EcoRI	GAATTC	1 cut(s)	5
EcoRII	CCWGG	2 cut(s)	670, 1429
EcoT14I	CCWWGG	1 cut(s)	1103
EcoT22I	ATGCAT	1 cut(s)	1600
EcoT38I	GRGCYC	1 cut(s)	322
ErhI	CCWWGG	1 cut(s)	1103
FaeI	CATG	2 cut(s)	619, 667
FalI	AAGNNNNNCTT	2 cut(s)	17, 49
FaqI	GGGAC	2 cut(s)	1290, 1325
FatI	CATG	2 cut(s)	615, 663
FblI	GTMKAC	2 cut(s)	96, 1440
FokI	GGATG	9 cut(s)	242, 257, 349, 386, 515, 1103, 1174, 1475, 1506
FriOI	GRGCYC	1 cut(s)	322
FspBI	CTAG	2 cut(s)	905, 1104
GsaI	CCCAGC	2 cut(s)	335, 983
GsuI	CTGGAG	1 cut(s)	1045
HaeIII	GGCC	2 cut(s)	670, 1628
HapII	CCGG	3 cut(s)	1335, 1523, 1625
Hin1II	CATG	2 cut(s)	619, 667
HindIII	AAGCTT	2 cut(s)	480, 1256
HinfI	GANTC	5 cut(s)	109, 152, 530, 653, 720
HpaII	CCGG	3 cut(s)	1335, 1523, 1625
HphI	GGTGA	2 cut(s)	439, 1538
Hpy166II	GTNNAC	3 cut(s)	97, 1289, 1441
Hpy188III	TCNNGA	5 cut(s)	290, 623, 637, 664, 848
Hpy8I	GTNNAC	3 cut(s)	97, 1289, 1441
HpyAV	CCTTC	4 cut(s)	127, 227, 1171, 1485
HpyCH4III	ACNGT	3 cut(s)	739, 1078, 1438
HpyCH4IV	ACGT	2 cut(s)	105, 788
HpyF10VI	GCNNNNNNNGC	7 cut(s)	213, 274, 602, 827, 944, 1482, 1485
HpyF3I	CTNAG	7 cut(s)	12, 382, 526, 561, 842, 1109, 1413
HpySE526I	ACGT	2 cut(s)	105, 788
Hsp92II	CATG	2 cut(s)	619, 667
KflI	GGGWCCC	1 cut(s)	1339
KpnI	GGTACC	1 cut(s)	1437
KroI	GCCGGC	1 cut(s)	1624
KroNI	GCCGGC	1 cut(s)	1626
Kzo9I	GATC	5 cut(s)	374, 387, 442, 544, 691
LguI	GCTCTTC	2 cut(s)	38, 306
LmnI	GCTCC	6 cut(s)	265, 960, 976, 1064, 1281, 1394
LweI	GCATC	4 cut(s)	1074, 1127, 1453, 1528
MabI	ACCWGGT	1 cut(s)	1429
MaeI	CTAG	2 cut(s)	905, 1104
MaeII	ACGT	2 cut(s)	105, 788
MaeIII	GTNAC	5 cut(s)	784, 845, 1069, 1207, 1360
MalI	GATC	5 cut(s)	376, 389, 444, 546, 693
MboI	GATC	5 cut(s)	374, 387, 442, 544, 691
MfeI	CAATTG	1 cut(s)	305
MflI	RGATCY	2 cut(s)	544, 691
MhlI	GDGCHC	1 cut(s)	322
MluCI	AATT	9 cut(s)	5, 77, 305, 420, 641, 1143, 1320, 1418, 1646
MmeI	TCCRAC	2 cut(s)	243, 427
Mph1103I	ATGCAT	1 cut(s)	1600
MroNI	GCCGGC	1 cut(s)	1624
MseI	TTAA	9 cut(s)	507, 894, 899, 972, 1149, 1346, 1383, 1601, 1654
MslI	CAYNNNNRTG	3 cut(s)	1524, 1578, 1593
MspA1I	CMGCKG	3 cut(s)	88, 935, 1479
MspI	CCGG	3 cut(s)	1335, 1523, 1625
MspR9I	CCNGG	5 cut(s)	672, 1335, 1336, 1431, 1524
MunI	CAATTG	1 cut(s)	305
MvaI	CCWGG	2 cut(s)	672, 1431
MwoI	GCNNNNNNNGC	7 cut(s)	213, 274, 602, 827, 944, 1482, 1485
NaeI	GCCGGC	1 cut(s)	1626
NciI	CCSGG	3 cut(s)	1335, 1336, 1524
NdeII	GATC	5 cut(s)	374, 387, 442, 544, 691
NgoMIV	GCCGGC	1 cut(s)	1624
NlaIII	CATG	2 cut(s)	619, 667
NlaIV	GGNNCC	4 cut(s)	1306, 1340, 1341, 1435
NmuCI	GTSAC	3 cut(s)	784, 845, 1069
NsiI	ATGCAT	1 cut(s)	1600
OliI	CACNNNNGTG	1 cut(s)	1578
PaeR7I	CTCGAG	1 cut(s)	290
PagI	TCATGA	1 cut(s)	663
PaqCI	CACCTGC	1 cut(s)	303
PceI	AGGCCT	1 cut(s)	670
PciSI	GCTCTTC	2 cut(s)	38, 306
PcsI	WCGNNNNNNNCGW	2 cut(s)	128, 161
PdiI	GCCGGC	1 cut(s)	1626
PfeI	GAWTC	5 cut(s)	109, 152, 530, 653, 720
Ppu21I	YACGTR	1 cut(s)	106
PpuMI	RGGWCCY	1 cut(s)	1339
PsiI	TTATAA	2 cut(s)	582, 752
Psp5II	RGGWCCY	1 cut(s)	1339
Psp6I	CCWGG	2 cut(s)	670, 1429
PspFI	CCCAGC	2 cut(s)	331, 979
PspGI	CCWGG	2 cut(s)	670, 1429
PspN4I	GGNNCC	4 cut(s)	1306, 1340, 1341, 1435
PspPI	GGNCC	2 cut(s)	1304, 1339
PspPPI	RGGWCCY	1 cut(s)	1339
PstI	CTGCAG	1 cut(s)	598
PsuI	RGATCY	2 cut(s)	544, 691
PvuII	CAGCTG	1 cut(s)	935
RsaI	GTAC	4 cut(s)	730, 1428, 1435, 1471
RsaNI	GTAC	4 cut(s)	729, 1427, 1434, 1470
RseI	CAYNNNNRTG	3 cut(s)	1524, 1578, 1593
SapI	GCTCTTC	2 cut(s)	38, 306
SaqAI	TTAA	9 cut(s)	507, 894, 899, 972, 1149, 1346, 1383, 1601, 1654
Sau3AI	GATC	5 cut(s)	374, 387, 442, 544, 691
Sau96I	GGNCC	2 cut(s)	1304, 1339
ScrFI	CCNGG	5 cut(s)	672, 1335, 1336, 1431, 1524
SduI	GDGCHC	1 cut(s)	322
SexAI	ACCWGGT	1 cut(s)	1429
SfaNI	GCATC	4 cut(s)	1074, 1127, 1453, 1528
SfcI	CTRYAG	2 cut(s)	594, 930
Sfr274I	CTCGAG	1 cut(s)	290
SinI	GGWCC	2 cut(s)	1304, 1339
SlaI	CTCGAG	1 cut(s)	290
SmaI	CCCGGG	1 cut(s)	1336
SmiMI	CAYNNNNRTG	3 cut(s)	1524, 1578, 1593
SmlI	CTYRAG	1 cut(s)	290
SmoI	CTYRAG	1 cut(s)	290
SpeI	ACTAGT	1 cut(s)	904
Sse9I	AATT	9 cut(s)	5, 77, 305, 420, 641, 1143, 1320, 1418, 1646
SseBI	AGGCCT	1 cut(s)	670
SsiI	CCGC	6 cut(s)	28, 88, 191, 821, 1479, 1482
SspMI	CTAG	2 cut(s)	905, 1104
StuI	AGGCCT	1 cut(s)	670
StyD4I	CCNGG	5 cut(s)	670, 1333, 1334, 1429, 1522
StyI	CCWWGG	1 cut(s)	1103
TaaI	ACNGT	3 cut(s)	739, 1078, 1438
TaiI	ACGT	2 cut(s)	108, 791
TaqI	TCGA	5 cut(s)	131, 164, 221, 291, 373
TasI	AATT	9 cut(s)	5, 77, 305, 420, 641, 1143, 1320, 1418, 1646
TauI	GCSGC	3 cut(s)	31, 1482, 1485
TfiI	GAWTC	5 cut(s)	109, 152, 530, 653, 720
Tru1I	TTAA	9 cut(s)	507, 894, 899, 972, 1149, 1346, 1383, 1601, 1654
Tru9I	TTAA	9 cut(s)	507, 894, 899, 972, 1149, 1346, 1383, 1601, 1654
TscAI	CASTG	2 cut(s)	664, 823
TseFI	GTSAC	3 cut(s)	784, 845, 1069
Tsp45I	GTSAC	3 cut(s)	784, 845, 1069
TspDTI	ATGAA	4 cut(s)	246, 795, 1365, 1653
TspGWI	ACGGA	4 cut(s)	421, 483, 713, 947
TspMI	CCCGGG	1 cut(s)	1334
TspRI	CASTG	2 cut(s)	664, 823
VpaK11BI	GGWCC	2 cut(s)	1304, 1339
XapI	RAATTY	6 cut(s)	5, 77, 641, 1143, 1320, 1646
XhoI	CTCGAG	1 cut(s)	290
XmaI	CCCGGG	1 cut(s)	1334
XmaJI	CCTAGG	1 cut(s)	1103
XmiI	GTMKAC	2 cut(s)	96, 1440
XspI	CTAG	2 cut(s)	905, 1104
Zsp2I	ATGCAT	1 cut(s)	1600

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.