Rorug06G0198300

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0005170
Physical Location & Seq
Forward (+)
31937558 .. 31940577
3020 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0198300.1

Sequence Viewer

Length: 831 bp
ATGGATCGGTATCAGAGGATAGAGAAGCCTCGGGAGGAAACGCCCATTAACGAGAATGAGATTCGCATTACCACTCAGGGCAGAATCAGGAACTACATTACCTACGCCACCACTCTCCTCCAGGAGAAAGGGTCCAATGAAATTTCTCTCAAAGCCATGGGCAGAGCTATCAACAAGACTGTGATGATCACTGAACTTATTAAGAGGAGGGTTGCCGGCCTTCATCAAAACACCTATATTGGCTCTACTGATATAACTGACATGTGGGAGCCACTAGAAGAAGGCCTTCTTCCCCTGGAAACTACTCGCCATGTTTCAGTGATTACAGTTACTTTGTCCAAGAAGGAATTGGACACGTCCTCTACAGGGTATCAGGGACCTATCCCAGCTGATCAAGTGAAACCATGGACTGAATATGATTATGAAGGACAGGGCCCTCCTAGTATGCGAGGAAGAGGACGTGGTCGAGGAAGGGGTAGACGAAGAGGGAATTTTAGTAACGGAGTGATGGTATACAATGCTGATGGCGGTTTCGATGGTGGTCGTGGCTATGGTGGGTTTGATGGTGGTCGTGGCTATGGTGGGTTTGAAGGTGGGCGTGGCTATGGTGGCAGGGGTCAGGGTCATGGAAGAGTCCGCGGTTATCGTGGGCGTGTGCGAGGATATGGCAGTGGAAGTATGCAACCAGAATCCGGTGGTTATACTGTTTATAATGATTATGGAGGTGGAGCACCTACAGCACAAGGGAGAGAGCGTGGACCAGGCAGAGCAAGGGGCGGTGGCCGCGGTGTAGGTGGTAGATTAGATGGACCTATCCAGAGTGCTGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

276

Amino Acids

29.96

Weight (kDa)

9.91

Isoelectric Point (pI)

25.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Alba PF01918 19 - 83 4.5e-17 Alba
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 711
AccI GTMKAC 2 cut(s) 478, 513
AccII CGCG 2 cut(s) 639, 786
AciI CCGC 6 cut(s) 528, 637, 639, 777, 784, 786
AclWI GGATC 1 cut(s) 12
AcoI YGGCCR 1 cut(s) 781
AcsI RAATTY 2 cut(s) 141, 490
AfiI CCNNNNNNNGG 2 cut(s) 366, 692
AflIII ACRYGT 2 cut(s) 261, 354
AgsI TTSAA 1 cut(s) 590
AjiI CACGTC 2 cut(s) 357, 461
AjnI CCWGG 3 cut(s) 120, 294, 760
AluBI AGCT 2 cut(s) 167, 389
AluI AGCT 2 cut(s) 167, 389
Alw21I GWGCWC 1 cut(s) 733
AlwI GGATC 1 cut(s) 12
Ama87I CYCGRG 1 cut(s) 30
AoxI GGCC 4 cut(s) 217, 283, 433, 781
ApaI GGGCCC 1 cut(s) 437
ApoI RAATTY 2 cut(s) 141, 490
Asp700I GAANNNNTTC 1 cut(s) 285
AspS9I GGNCC 6 cut(s) 132, 377, 433, 434, 758, 809
AvaI CYCGRG 1 cut(s) 30
AvaII GGWCC 4 cut(s) 132, 377, 758, 809
BaeGI GKGCMC 1 cut(s) 437
BanII GRGCYC 1 cut(s) 437
Bbv12I GWGCWC 1 cut(s) 733
BccI CCATC 5 cut(s) 502, 518, 530, 557, 800
BciT130I CCWGG 3 cut(s) 122, 296, 762
BclI TGATCA 2 cut(s) 186, 391
BfaI CTAG 2 cut(s) 275, 441
BfmI CTRYAG 2 cut(s) 363, 735
BisI GCNGC 1 cut(s) 784
BlsI GCNGC 1 cut(s) 785
Bme1390I CCNGG 3 cut(s) 122, 296, 762
Bme18I GGWCC 4 cut(s) 132, 377, 758, 809
BmeT110I CYCGRG 1 cut(s) 30
BmgBI CACGTC 2 cut(s) 357, 461
BmgT120I GGNCC 6 cut(s) 132, 377, 433, 434, 758, 809
BmiI GGNNCC 4 cut(s) 133, 270, 378, 435
BmrFI CCNGG 3 cut(s) 122, 296, 762
BpmI CTGGAG 1 cut(s) 104
BsaBI GATNNNNATC 1 cut(s) 9
BsaJI CCNNGG 6 cut(s) 29, 156, 294, 404, 637, 784
BsaWI WCCGGW 1 cut(s) 692
BsaXI ACNNNNNCTCC 6 cut(s) 116, 146, 495, 525, 739, 769
Bsc4I CCNNNNNNNGG 2 cut(s) 366, 692
Bse118I RCCGGY 1 cut(s) 215
Bse8I GATNNNNATC 1 cut(s) 9
BseBI CCWGG 3 cut(s) 122, 296, 762
BseDI CCNNGG 6 cut(s) 29, 156, 294, 404, 637, 784
BseJI GATNNNNATC 1 cut(s) 9
BseLI CCNNNNNNNGG 2 cut(s) 366, 692
BseMII CTCAG 1 cut(s) 89
BseRI GAGGAG 2 cut(s) 107, 220
BseSI GKGCMC 1 cut(s) 437
BseYI CCCAGC 1 cut(s) 385
Bsh1236I CGCG 2 cut(s) 639, 786
BshFI GGCC 4 cut(s) 219, 285, 435, 783
BsiHKAI GWGCWC 1 cut(s) 733
BsiHKCI CYCGRG 1 cut(s) 30
BsiSI CCGG 2 cut(s) 216, 693
BslFI GGGAC 1 cut(s) 390
BslI CCNNNNNNNGG 2 cut(s) 366, 692
BsmFI GGGAC 1 cut(s) 390
BsnI GGCC 4 cut(s) 219, 285, 435, 783
BsoBI CYCGRG 1 cut(s) 30
Bsp120I GGGCCC 1 cut(s) 433
Bsp1286I GDGCHC 2 cut(s) 437, 733
Bsp143I GATC 3 cut(s) 4, 186, 391
Bsp19I CCATGG 2 cut(s) 156, 404
BspACI CCGC 6 cut(s) 528, 637, 639, 777, 784, 786
BspANI GGCC 4 cut(s) 219, 285, 435, 783
BspCNI CTCAG 1 cut(s) 88
BspFNI CGCG 2 cut(s) 639, 786
BspLI GGNNCC 4 cut(s) 133, 270, 378, 435
BspPI GGATC 1 cut(s) 12
BsrFI RCCGGY 1 cut(s) 215
BssAI RCCGGY 1 cut(s) 215
BssECI CCNNGG 6 cut(s) 29, 156, 294, 404, 637, 784
BssMI GATC 3 cut(s) 4, 186, 391
BssNAI GTATAC 1 cut(s) 514
BssT1I CCWWGG 2 cut(s) 156, 404
Bst1107I GTATAC 1 cut(s) 514
Bst2UI CCWGG 3 cut(s) 122, 296, 762
Bst4CI ACNGT 3 cut(s) 181, 328, 706
Bst6I CTCTTC 3 cut(s) 448, 478, 625
BstC8I GCNNGC 1 cut(s) 217
BstDEI CTNAG 1 cut(s) 75
BstDSI CCRYGG 4 cut(s) 156, 404, 637, 784
BstFNI CGCG 2 cut(s) 639, 786
BstKTI GATC 3 cut(s) 7, 189, 394
BstMBI GATC 3 cut(s) 4, 186, 391
BstMWI GCNNNNNNNGC 3 cut(s) 609, 737, 783
BstNI CCWGG 3 cut(s) 122, 296, 762
BstNSI RCATGY 1 cut(s) 265
BstSCI CCNGG 3 cut(s) 120, 294, 760
BstSFI CTRYAG 2 cut(s) 363, 735
BstSLI GKGCMC 1 cut(s) 437
BstUI CGCG 2 cut(s) 639, 786
BstZ17I GTATAC 1 cut(s) 514
BsuRI GGCC 4 cut(s) 219, 285, 435, 783
BtgI CCRYGG 4 cut(s) 156, 404, 637, 784
BtrI CACGTC 2 cut(s) 357, 461
BtsI GCAGTG 1 cut(s) 676
BtsIMutI CAGTG 3 cut(s) 189, 324, 676
Cac8I GCNNGC 1 cut(s) 217
Cfr10I RCCGGY 1 cut(s) 215
Cfr13I GGNCC 6 cut(s) 132, 377, 433, 434, 758, 809
Cfr42I CCGCGG 2 cut(s) 640, 787
CviAII CATG 5 cut(s) 157, 262, 311, 405, 626
DdeI CTNAG 1 cut(s) 75
DpnI GATC 3 cut(s) 6, 188, 393
DpnII GATC 3 cut(s) 4, 186, 391
EaeI YGGCCR 1 cut(s) 781
Eam1104I CTCTTC 3 cut(s) 448, 478, 625
EarI CTCTTC 3 cut(s) 448, 478, 625
Eco130I CCWWGG 2 cut(s) 156, 404
Eco147I AGGCCT 1 cut(s) 285
Eco24I GRGCYC 1 cut(s) 437
Eco47I GGWCC 4 cut(s) 132, 377, 758, 809
Eco88I CYCGRG 1 cut(s) 30
EcoO109I RGGNCCY 3 cut(s) 377, 433, 434
EcoRII CCWGG 3 cut(s) 120, 294, 760
EcoT14I CCWWGG 2 cut(s) 156, 404
EcoT38I GRGCYC 1 cut(s) 437
ErhI CCWWGG 2 cut(s) 156, 404
FaeI CATG 5 cut(s) 160, 265, 314, 408, 629
FalI AAGNNNNNCTT 4 cut(s) 270, 302, 273, 305
FaqI GGGAC 1 cut(s) 390
FatI CATG 5 cut(s) 156, 261, 310, 404, 625
FbaI TGATCA 2 cut(s) 186, 391
FblI GTMKAC 2 cut(s) 478, 513
Fnu4HI GCNGC 1 cut(s) 784
FriOI GRGCYC 1 cut(s) 437
Fsp4HI GCNGC 1 cut(s) 784
FspBI CTAG 2 cut(s) 275, 441
GluI GCNGC 1 cut(s) 784
GsaI CCCAGC 1 cut(s) 389
GsuI CTGGAG 1 cut(s) 104
HaeIII GGCC 4 cut(s) 219, 285, 435, 783
HapII CCGG 2 cut(s) 216, 693
Hin1II CATG 5 cut(s) 160, 265, 314, 408, 629
HinfI GANTC 4 cut(s) 61, 84, 633, 689
HpaII CCGG 2 cut(s) 216, 693
Hpy166II GTNNAC 3 cut(s) 479, 514, 758
Hpy188I TCNGA 1 cut(s) 15
Hpy188III TCNNGA 3 cut(s) 32, 88, 817
Hpy8I GTNNAC 3 cut(s) 479, 514, 758
HpyAV CCTTC 7 cut(s) 230, 275, 296, 337, 419, 465, 584
HpyCH4III ACNGT 3 cut(s) 181, 328, 706
HpyCH4IV ACGT 2 cut(s) 356, 460
HpyCH4V TGCA 1 cut(s) 682
HpyF10VI GCNNNNNNNGC 3 cut(s) 609, 737, 783
HpyF3I CTNAG 1 cut(s) 75
HpySE526I ACGT 2 cut(s) 356, 460
Hsp92II CATG 5 cut(s) 160, 265, 314, 408, 629
KroI GCCGGC 1 cut(s) 215
KroNI GCCGGC 1 cut(s) 217
Ksp22I TGATCA 2 cut(s) 186, 391
KspI CCGCGG 2 cut(s) 640, 787
Kzo9I GATC 3 cut(s) 4, 186, 391
LmnI GCTCC 2 cut(s) 268, 728
MaeI CTAG 2 cut(s) 275, 441
MaeII ACGT 2 cut(s) 356, 460
MaeIII GTNAC 2 cut(s) 328, 497
MalI GATC 3 cut(s) 6, 188, 393
MboI GATC 3 cut(s) 4, 186, 391
MboII GAAGA 5 cut(s) 281, 290, 465, 495, 642
MhlI GDGCHC 2 cut(s) 437, 733
MluCI AATT 3 cut(s) 141, 347, 490
MlyI GAGTC 1 cut(s) 642
MroNI GCCGGC 1 cut(s) 215
MroXI GAANNNNTTC 1 cut(s) 285
MseI TTAA 2 cut(s) 48, 201
MspA1I CMGCKG 3 cut(s) 389, 639, 786
MspI CCGG 2 cut(s) 216, 693
MspR9I CCNGG 3 cut(s) 122, 296, 762
MvaI CCWGG 3 cut(s) 122, 296, 762
MvnI CGCG 2 cut(s) 639, 786
MwoI GCNNNNNNNGC 3 cut(s) 609, 737, 783
NaeI GCCGGC 1 cut(s) 217
NcoI CCATGG 2 cut(s) 156, 404
NdeII GATC 3 cut(s) 4, 186, 391
NgoMIV GCCGGC 1 cut(s) 215
NlaIII CATG 5 cut(s) 160, 265, 314, 408, 629
NlaIV GGNNCC 4 cut(s) 133, 270, 378, 435
NspI RCATGY 1 cut(s) 265
PceI AGGCCT 1 cut(s) 285
PciI ACATGT 1 cut(s) 261
PdiI GCCGGC 1 cut(s) 217
PdmI GAANNNNTTC 1 cut(s) 285
PfeI GAWTC 3 cut(s) 61, 84, 689
PflFI GACNNNGTC 1 cut(s) 462
PfoI TCCNGGA 1 cut(s) 120
PkrI GCNGC 1 cut(s) 785
PleI GAGTC 1 cut(s) 641
PpsI GAGTC 1 cut(s) 641
PpuMI RGGWCCY 1 cut(s) 377
PscI ACATGT 1 cut(s) 261
PsiI TTATAA 1 cut(s) 711
Psp5II RGGWCCY 1 cut(s) 377
Psp6I CCWGG 3 cut(s) 120, 294, 760
PspFI CCCAGC 1 cut(s) 385
PspGI CCWGG 3 cut(s) 120, 294, 760
PspN4I GGNNCC 4 cut(s) 133, 270, 378, 435
PspOMI GGGCCC 1 cut(s) 433
PspPI GGNCC 6 cut(s) 132, 377, 433, 434, 758, 809
PspPPI RGGWCCY 1 cut(s) 377
PsyI GACNNNGTC 1 cut(s) 462
PvuII CAGCTG 1 cut(s) 389
SacII CCGCGG 2 cut(s) 640, 787
SaqAI TTAA 2 cut(s) 48, 201
SatI GCNGC 1 cut(s) 784
Sau3AI GATC 3 cut(s) 4, 186, 391
Sau96I GGNCC 6 cut(s) 132, 377, 433, 434, 758, 809
SchI GAGTC 1 cut(s) 642
ScrFI CCNGG 3 cut(s) 122, 296, 762
SduI GDGCHC 2 cut(s) 437, 733
SfcI CTRYAG 2 cut(s) 363, 735
Sfr303I CCGCGG 2 cut(s) 640, 787
SgrBI CCGCGG 2 cut(s) 640, 787
SinI GGWCC 4 cut(s) 132, 377, 758, 809
Sse9I AATT 3 cut(s) 141, 347, 490
SseBI AGGCCT 1 cut(s) 285
SsiI CCGC 6 cut(s) 528, 637, 639, 777, 784, 786
SspMI CTAG 2 cut(s) 275, 441
StuI AGGCCT 1 cut(s) 285
StyD4I CCNGG 3 cut(s) 120, 294, 760
StyI CCWWGG 2 cut(s) 156, 404
TaaI ACNGT 3 cut(s) 181, 328, 706
TaiI ACGT 2 cut(s) 359, 463
TaqI TCGA 2 cut(s) 466, 534
TasI AATT 3 cut(s) 141, 347, 490
TauI GCSGC 1 cut(s) 786
TfiI GAWTC 3 cut(s) 61, 84, 689
Tru1I TTAA 2 cut(s) 48, 201
Tru9I TTAA 2 cut(s) 48, 201
TscAI CASTG 3 cut(s) 196, 324, 676
TspDTI ATGAA 3 cut(s) 153, 212, 438
TspGWI ACGGA 1 cut(s) 516
TspRI CASTG 3 cut(s) 196, 324, 676
Tth111I GACNNNGTC 1 cut(s) 462
VpaK11BI GGWCC 4 cut(s) 132, 377, 758, 809
XapI RAATTY 2 cut(s) 141, 490
XceI RCATGY 1 cut(s) 265
XcmI CCANNNNNNNNNTGG 1 cut(s) 346
XmiI GTMKAC 2 cut(s) 478, 513
XmnI GAANNNNTTC 1 cut(s) 285
XspI CTAG 2 cut(s) 275, 441
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.