MD05G1014100.v1.1

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0005170
Physical Location & Seq
Forward (+)
2635671 .. 2636284
614 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1014100.v1.1.491

Sequence Viewer

Length: 384 bp
ATGGTAGCTACCAAAACAAATTACGACCAGAAAAGTGAGGTAAAGGCTTTTGATGACACCAAAGAAGGAGTTAAAGGCCTCGTTGATGCGGGCATAACCGAGGTTCCTAGAATTTTTCATCAACCACTGGAGTCGGATATTGGGGATCGGGGGAGATTTAAGAGCATACGACACAAGGTACTGGCAAACTGTGATGGCCCAAGGATATCTGTTGCGAGCTTCTTCTATACAGGGATGCTACCGTTGACAAAACTCTATGGACCAATCAAAGAGTTATTAGACAATCATCCAACGTACAGGGAGACAACTGTGAGGGATTTCTTTGTTTACTACAACAAAAAAGGCCTTGATGACACATCTGGCTTGTCGGATCTCGAGCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

128

Amino Acids

14.36

Weight (kDa)

6.28

Isoelectric Point (pI)

14.35

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 89
AclWI GGATC 2 cut(s) 153, 378
AcsI RAATTY 1 cut(s) 111
AfaI GTAC 2 cut(s) 180, 296
AluBI AGCT 3 cut(s) 8, 219, 379
AluI AGCT 3 cut(s) 8, 219, 379
Alw26I GTCTC 1 cut(s) 296
AlwI GGATC 2 cut(s) 153, 378
Ama87I CYCGRG 1 cut(s) 374
AoxI GGCC 3 cut(s) 76, 196, 343
ApoI RAATTY 1 cut(s) 111
AspS9I GGNCC 2 cut(s) 197, 260
AvaI CYCGRG 1 cut(s) 374
AvaII GGWCC 1 cut(s) 260
BccI CCATC 1 cut(s) 188
BcoDI GTCTC 1 cut(s) 296
BfaI CTAG 1 cut(s) 108
Bme18I GGWCC 1 cut(s) 260
BmeT110I CYCGRG 1 cut(s) 374
BmgT120I GGNCC 2 cut(s) 197, 260
BmiI GGNNCC 1 cut(s) 105
BmsI GCATC 2 cut(s) 76, 225
BpmI CTGGAG 1 cut(s) 149
BsaJI CCNNGG 2 cut(s) 99, 200
Bse1I ACTGG 2 cut(s) 132, 186
BseDI CCNNGG 2 cut(s) 99, 200
BseGI GGATG 2 cut(s) 240, 286
BseNI ACTGG 2 cut(s) 132, 186
BshFI GGCC 3 cut(s) 78, 198, 345
BsiHKCI CYCGRG 1 cut(s) 374
BsmAI GTCTC 1 cut(s) 296
BsnI GGCC 3 cut(s) 78, 198, 345
BsoBI CYCGRG 1 cut(s) 374
Bsp143I GATC 2 cut(s) 145, 370
BspACI CCGC 1 cut(s) 89
BspANI GGCC 3 cut(s) 78, 198, 345
BspLI GGNNCC 1 cut(s) 105
BspPI GGATC 2 cut(s) 153, 378
BsrI ACTGG 2 cut(s) 132, 186
BssECI CCNNGG 2 cut(s) 99, 200
BssMI GATC 2 cut(s) 145, 370
BssT1I CCWWGG 1 cut(s) 200
Bst4CI ACNGT 3 cut(s) 191, 243, 310
BstC8I GCNNGC 2 cut(s) 91, 217
BstF5I GGATG 2 cut(s) 240, 286
BstKTI GATC 2 cut(s) 148, 373
BstMAI GTCTC 1 cut(s) 296
BstMBI GATC 2 cut(s) 145, 370
BstX2I RGATCY 1 cut(s) 370
BstYI RGATCY 1 cut(s) 370
BsuRI GGCC 3 cut(s) 78, 198, 345
BtsCI GGATG 2 cut(s) 240, 286
BtsIMutI CAGTG 1 cut(s) 125
Cac8I GCNNGC 2 cut(s) 91, 217
Cfr13I GGNCC 2 cut(s) 197, 260
Csp6I GTAC 2 cut(s) 179, 295
CviJI RGCY 8 cut(s) 8, 47, 78, 198, 219, 345, 363, 379
CviKI_1 RGCY 8 cut(s) 8, 47, 78, 198, 219, 345, 363, 379
CviQI GTAC 2 cut(s) 179, 295
DpnI GATC 2 cut(s) 147, 372
DpnII GATC 2 cut(s) 145, 370
Eco130I CCWWGG 1 cut(s) 200
Eco147I AGGCCT 2 cut(s) 78, 345
Eco32I GATATC 1 cut(s) 207
Eco47I GGWCC 1 cut(s) 260
Eco88I CYCGRG 1 cut(s) 374
EcoRV GATATC 1 cut(s) 207
EcoT14I CCWWGG 1 cut(s) 200
ErhI CCWWGG 1 cut(s) 200
FaiI YATR 4 cut(s) 95, 167, 228, 258
FauI CCCGC 1 cut(s) 82
FokI GGATG 2 cut(s) 247, 273
FspBI CTAG 1 cut(s) 108
GsuI CTGGAG 1 cut(s) 149
HaeIII GGCC 3 cut(s) 78, 198, 345
HincII GTYRAC 1 cut(s) 246
HindII GTYRAC 1 cut(s) 246
HinfI GANTC 1 cut(s) 131
Hpy166II GTNNAC 2 cut(s) 246, 328
Hpy188I TCNGA 2 cut(s) 136, 370
Hpy188III TCNNGA 1 cut(s) 374
Hpy8I GTNNAC 2 cut(s) 246, 328
HpyAV CCTTC 1 cut(s) 59
HpyCH4III ACNGT 3 cut(s) 191, 243, 310
HpyCH4IV ACGT 1 cut(s) 293
HpySE526I ACGT 1 cut(s) 293
Kzo9I GATC 2 cut(s) 145, 370
LpnPI CCDG 6 cut(s) 41, 113, 167, 216, 283, 345
LweI GCATC 2 cut(s) 76, 225
MaeI CTAG 1 cut(s) 108
MaeII ACGT 1 cut(s) 293
MalI GATC 2 cut(s) 147, 372
MboI GATC 2 cut(s) 145, 370
MboII GAAGA 1 cut(s) 214
MflI RGATCY 1 cut(s) 370
MluCI AATT 2 cut(s) 19, 111
MlyI GAGTC 1 cut(s) 140
MmeI TCCRAC 3 cut(s) 114, 314, 348
MnlI CCTC 4 cut(s) 31, 89, 94, 306
MseI TTAA 2 cut(s) 72, 159
NdeII GATC 2 cut(s) 145, 370
NlaIV GGNNCC 1 cut(s) 105
PaeR7I CTCGAG 1 cut(s) 374
PceI AGGCCT 2 cut(s) 78, 345
PleI GAGTC 1 cut(s) 139
PpsI GAGTC 1 cut(s) 139
PspN4I GGNNCC 1 cut(s) 105
PspPI GGNCC 2 cut(s) 197, 260
PsuI RGATCY 1 cut(s) 370
RsaI GTAC 2 cut(s) 180, 296
RsaNI GTAC 2 cut(s) 179, 295
SaqAI TTAA 2 cut(s) 72, 159
Sau3AI GATC 2 cut(s) 145, 370
Sau96I GGNCC 2 cut(s) 197, 260
SchI GAGTC 1 cut(s) 140
SetI ASST 7 cut(s) 10, 42, 105, 180, 221, 296, 381
SfaNI GCATC 2 cut(s) 76, 225
Sfr274I CTCGAG 1 cut(s) 374
SinI GGWCC 1 cut(s) 260
SlaI CTCGAG 1 cut(s) 374
SmlI CTYRAG 1 cut(s) 374
SmoI CTYRAG 1 cut(s) 374
Sse9I AATT 2 cut(s) 19, 111
SseBI AGGCCT 2 cut(s) 78, 345
SsiI CCGC 1 cut(s) 89
SspMI CTAG 1 cut(s) 108
StuI AGGCCT 2 cut(s) 78, 345
StyI CCWWGG 1 cut(s) 200
TaaI ACNGT 3 cut(s) 191, 243, 310
TaiI ACGT 1 cut(s) 296
TaqI TCGA 1 cut(s) 375
TasI AATT 2 cut(s) 19, 111
Tru1I TTAA 2 cut(s) 72, 159
Tru9I TTAA 2 cut(s) 72, 159
TscAI CASTG 1 cut(s) 132
TspDTI ATGAA 1 cut(s) 107
TspRI CASTG 1 cut(s) 132
VpaK11BI GGWCC 1 cut(s) 260
XapI RAATTY 1 cut(s) 111
XhoI CTCGAG 1 cut(s) 374
XspI CTAG 1 cut(s) 108
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.