Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr6C

Orthogroup ID

OG0012096

Physical Location & Seq

Genomic Coordinates Forward (+)

50911598 .. 50915390

Gene Structure 3793 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh6CG323000.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1650 bp

ATGGAAATGGCCAAGCATTCAGTGAAGCGTCTGCTCTGCAATTCACATTTCACGCAAAGCTTGATGAAGAGAGAGGAATCATACCGGAGGTTGATATATAGCTTCGTGAATCGGTTTCAATCGCAAAGGTTGAAAGCAGACAAGGTGGATTCAGAGTCACTCAAGGAATCAAATGTTGCTGTTCATCCTCATCAAGCTTCTCTGAGGCTTAGAAAGGATGGCATTGGTAGTTATAGTAGTGGGGATGAAGATTTGAGCGAGAATAAGGAGAAAAGGTTAGAGCTTTCATGGCTTCCCAAAGCGCTTGAGCCAGCTCTGCAGTTGTGCAGGTGGGCTCTGACAGGCCCAACAGGGAACGGGGTGGGAAACAAACCCCCACCAAGTACTCGATCTGTCTCAGAGATCATTGCAAGCATCCAACGCAGTAAGACAGGCATTCAAGATTGGAGTTTGAGTGATCTCACTGTTGGTTTATTTCTTATTTATCTTCGTCAAGCATCTGTAAATCCATTTGAGGATGTTACTGGCATTCAGATCTCATCAGATAGATTGGTACATGACCTTATTTACCACGTTGAGTTGGCAAAAGGTTGTTATAAGGACAATGCTGCAGCTCTCGCAAGGAACTGCATGCTTCGAGAAAGCAATGTCTTGAAGTTTGTCAAATATTCTAGTGTCATGAGGCCGGGTTATTATATAGGAATTGATACTCGTAGAAAACTTGTGATTCTAGGAATCCGTGGAACTCATACTGTGTATGACCTTATTACTGACGTTGTTTCTTCAAGTGACGGTGAAGTCACTCTTGAAGGGTTTTCAACCCACTTTGGCACTGCAGAAGCTGCTCGTTGGTTTCTTACTCACGAAATGGGAAATATAAGGAAGTGCTTGGAGAAGTATGAGGGATTTAAGTTAAGGCTTGTGGGTCATTCTCTTGGAGGTGCTACAGCTGCTCTACTAGCAATAATGCTCCGTAAAAAGTCGTATAAGGAGCTAGGGTTTAGCCCAGAGATTGTTTCTGCTGTCGGATATGCAGCGCCACCTTGTGTCTCCAAAGAACTTGCTGAAAGCTGCTCCAGTTATGTCAGAACTGTTGTGATGCAGGATGATATTATACCTAGACTTAGTGTAGCATCTCTTATGAGGTTGAGGAATGAAATTCTTCAAACTGATTGGATGAGTGTGATTGAGAAGGAAGACTGGAGAAGTGTCATAGATTTGGTGACAAATGCAAAACAGGTTGTATCTTCGGTGCAAGATGTTGCGGCAAAACTTGCTGATTATGCAAAATTCAGGAGCAATAAAGATTCCCCTGATGATCCGATGATAAAGAAGGCGGCTGCTGCTTCCAGAGCCCCTTTAAATGACAAAACTGTAAGAGATAATGCTGTTGGTAGTCCAAATGCTGCCAGTAGAGTGCCTGAGGAGTTATTCGTACCAGGTACTGTTTACTATTTAAAGAGGAATGTGGATGCTGATACTAGCGATTATTTTACACTTTGGAAGAGGAATCCGGGTGAACATTTTCAAAGGATTGTGCTTTCAAGCAACTTAATATCGGACCACAAGTGTGATAGCCATTATTATGCATTAAGAGATGTACTCAAAGGTTTGCCCTCCTCCAATGATGAAGGCAATTCTCTTAATTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001505 GO:0001676 GO:0003674 GO:0003824 GO:0005575 GO:0005623 GO:0005886 GO:0006082 GO:0006629 GO:0006631 GO:0006638 GO:0006639 GO:0006690 GO:0008150 GO:0008152 GO:0009056 GO:0009058 GO:0009987 GO:0016020 GO:0016042 GO:0016298 GO:0016787 GO:0016788 GO:0019369 GO:0019752 GO:0032787 GO:0033559 GO:0042133 GO:0042136 GO:0043436 GO:0044237 GO:0044238 GO:0044242 GO:0044248 GO:0044249 GO:0044255 GO:0044281 GO:0044464 GO:0046339 GO:0046340 GO:0046461 GO:0046464 GO:0046486 GO:0046503 GO:0047372 GO:0052689 GO:0065007 GO:0065008 GO:0071704 GO:0071944 GO:1901568 GO:1901575

KEGG Pathways

Metabolic & Signaling

ko04723 ko04745 ko04925 map04723 map04745 map04925

Pfam Domains

Protein Families

Lipase_3

Protein Analysis

549

Amino Acids

61.35

Weight (kDa)

8.85

Isoelectric Point (pI)

46.68

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Lipase_3	PF01764	244 - 377	2.8e-26	Lipase (class 3)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0012096)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G42450
fragaria_vesca	FvH4_2g21920 FvH4_2g21920
malus_domestica	MD05G1200600.v1.1 MD10G1187200.v1.1
prunus_persica	Prupe.8G227200_v2.0.a1
pyrus_communis	pycom05g18740
rosa_chinensis	RchiOBHm_Chr6g0288821
rosa_laevigata	RLG00000012365
rosa_multiflora	Rmu_sc0005314.1_g000019
rosa_roxburghii	Rroxscaffold_7G00179870
rosa_rugosa	Rorug06G0198300 Rorug06G0198300 Rorug06G0198300
rosa_samantha	Rh6AG309600 Rh6BG315500 Rh6CG323000 Rh6DG308700
rosa_wichuraiana	Rw6G026760

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	597
AarI	CACCTGC	1 cut(s)	318
AasI	GACNNNNNNGTC	1 cut(s)	797
Acc36I	ACCTGC	1 cut(s)	318
AciI	CCGC	2 cut(s)	1265, 1337
AclWI	GGATC	1 cut(s)	1313
AcoI	YGGCCR	1 cut(s)	9
AcsI	RAATTY	2 cut(s)	1158, 1289
AdeI	CACNNNGTG	1 cut(s)	1046
AfaI	GTAC	5 cut(s)	385, 555, 1437, 1444, 1602
AfeI	AGCGCT	1 cut(s)	303
AjnI	CCWGG	1 cut(s)	1438
AleI	CACNNNNGTG	1 cut(s)	1569
Alw26I	GTCTC	2 cut(s)	400, 1054
AlwI	GGATC	1 cut(s)	1313
AlwNI	CAGNNNCTG	2 cut(s)	842, 1445
Aor51HI	AGCGCT	1 cut(s)	303
AoxI	GGCC	3 cut(s)	9, 343, 683
ApeKI	GCWGC	9 cut(s)	608, 611, 842, 950, 1034, 1071, 1340, 1343, 1406
ApoI	RAATTY	2 cut(s)	1158, 1289
Asp700I	GAANNNNTTC	2 cut(s)	1161, 1524
AspLEI	GCGC	2 cut(s)	304, 1039
AspS9I	GGNCC	2 cut(s)	344, 1561
AsuC2I	CCSGG	2 cut(s)	687, 1515
AsuHPI	GGTGA	3 cut(s)	806, 1234, 1529
AvaII	GGWCC	1 cut(s)	1561
AxyI	CCTNAGG	1 cut(s)	1422
BalI	TGGCCA	1 cut(s)	11
BanII	GRGCYC	2 cut(s)	337, 1357
BbsI	GAAGAC	1 cut(s)	1203
BbvI	GCAGC	9 cut(s)	595, 623, 829, 937, 1046, 1058, 1327, 1330, 1393
BccI	CCATC	1 cut(s)	212
BciT130I	CCWGG	1 cut(s)	1440
BcnI	CCSGG	2 cut(s)	687, 1515
BcoDI	GTCTC	2 cut(s)	400, 1054
BfaI	CTAG	6 cut(s)	672, 731, 959, 995, 1119, 1482
BfmI	CTRYAG	4 cut(s)	317, 609, 834, 945
BfoI	RGCGCY	2 cut(s)	305, 1040
BfuAI	ACCTGC	1 cut(s)	318
BglII	AGATCT	1 cut(s)	534
BmcAI	AGTACT	1 cut(s)	385
Bme1390I	CCNGG	3 cut(s)	687, 1440, 1515
Bme18I	GGWCC	1 cut(s)	1561
BmgT120I	GGNCC	2 cut(s)	344, 1561
BmrFI	CCNGG	3 cut(s)	687, 1440, 1515
BmsI	GCATC	5 cut(s)	423, 506, 1089, 1142, 1462
BpiI	GAAGAC	1 cut(s)	1203
BplI	GAGNNNNNCTC	2 cut(s)	1587, 1619
BpmI	CTGGAG	2 cut(s)	1060, 1222
BpuEI	CTTGAG	2 cut(s)	146, 326
BpuMI	CCSGG	2 cut(s)	687, 1515
BsaJI	CCNNGG	1 cut(s)	739
BsaWI	WCCGGW	1 cut(s)	84
BsaXI	ACNNNNNCTCC	6 cut(s)	260, 290, 439, 469, 983, 1013
Bse1I	ACTGG	4 cut(s)	529, 1077, 1205, 1410
Bse21I	CCTNAGG	1 cut(s)	1422
Bse3DI	GCAATG	2 cut(s)	405, 652
BseBI	CCWGG	1 cut(s)	1440
BseDI	CCNNGG	1 cut(s)	739
BseGI	GGATG	8 cut(s)	184, 223, 250, 414, 523, 1111, 1182, 1477
BseMI	GCAATG	2 cut(s)	405, 652
BseMII	CTCAG	3 cut(s)	194, 411, 1413
BseNI	ACTGG	4 cut(s)	529, 1077, 1205, 1410
BseRI	GAGGAG	2 cut(s)	1439, 1609
BseXI	GCAGC	9 cut(s)	595, 623, 829, 937, 1046, 1058, 1327, 1330, 1393
BsgI	GTGCAG	1 cut(s)	346
BshFI	GGCC	3 cut(s)	11, 345, 685
BsiSI	CCGG	3 cut(s)	85, 686, 1514
BsmAI	GTCTC	2 cut(s)	400, 1054
BsmI	GAATGC	3 cut(s)	16, 435, 528
BsnI	GGCC	3 cut(s)	11, 345, 685
Bsp1286I	GDGCHC	2 cut(s)	337, 1357
Bsp143I	GATC	5 cut(s)	389, 402, 457, 534, 1318
BspACI	CCGC	2 cut(s)	1265, 1337
BspANI	GGCC	3 cut(s)	11, 345, 685
BspCNI	CTCAG	3 cut(s)	195, 410, 1414
BspHI	TCATGA	1 cut(s)	678
BspMAI	CTGCAG	3 cut(s)	321, 613, 838
BspMI	ACCTGC	1 cut(s)	318
BspPI	GGATC	1 cut(s)	1313
BsrDI	GCAATG	2 cut(s)	405, 652
BsrI	ACTGG	4 cut(s)	529, 1077, 1205, 1410
BssECI	CCNNGG	1 cut(s)	739
BssMI	GATC	5 cut(s)	389, 402, 457, 534, 1318
Bst2UI	CCWGG	1 cut(s)	1440
Bst4CI	ACNGT	6 cut(s)	466, 754, 794, 1093, 1375, 1447
Bst6I	CTCTTC	2 cut(s)	62, 1499
BstAPI	GCANNNNNTGC	2 cut(s)	842, 1274
BstC8I	GCNNGC	3 cut(s)	312, 412, 632
BstDEI	CTNAG	5 cut(s)	203, 209, 397, 1124, 1422
BstDSI	CCRYGG	1 cut(s)	739
BstF5I	GGATG	8 cut(s)	184, 223, 250, 414, 523, 1111, 1182, 1477
BstH2I	RGCGCY	2 cut(s)	305, 1040
BstHHI	GCGC	2 cut(s)	304, 1039
BstKTI	GATC	5 cut(s)	392, 405, 460, 537, 1321
BstMAI	GTCTC	2 cut(s)	400, 1054
BstMBI	GATC	5 cut(s)	389, 402, 457, 534, 1318
BstNI	CCWGG	1 cut(s)	1440
BstNSI	RCATGY	1 cut(s)	634
BstSCI	CCNGG	3 cut(s)	685, 1438, 1513
BstSFI	CTRYAG	4 cut(s)	317, 609, 834, 945
BstV1I	GCAGC	9 cut(s)	595, 623, 829, 937, 1046, 1058, 1327, 1330, 1393
BstV2I	GAAGAC	1 cut(s)	1203
BstX2I	RGATCY	1 cut(s)	534
BstYI	RGATCY	1 cut(s)	534
Bsu36I	CCTNAGG	1 cut(s)	1422
BsuRI	GGCC	3 cut(s)	11, 345, 685
BtgI	CCRYGG	1 cut(s)	739
BtsCI	GGATG	8 cut(s)	184, 223, 250, 414, 523, 1111, 1182, 1477
BtsI	GCAGTG	1 cut(s)	831
BtsIMutI	CAGTG	3 cut(s)	27, 462, 831
BveI	ACCTGC	1 cut(s)	318
Cac8I	GCNNGC	3 cut(s)	312, 412, 632
CaiI	CAGNNNCTG	2 cut(s)	842, 1445
CciI	TCATGA	1 cut(s)	678
CfoI	GCGC	2 cut(s)	304, 1039
Cfr13I	GGNCC	2 cut(s)	344, 1561
CseI	GACGC	1 cut(s)	17
CsiI	ACCWGGT	1 cut(s)	1438
Csp6I	GTAC	5 cut(s)	384, 554, 1436, 1443, 1601
CviAII	CATG	4 cut(s)	288, 557, 631, 679
CviQI	GTAC	5 cut(s)	384, 554, 1436, 1443, 1601
DdeI	CTNAG	5 cut(s)	203, 209, 397, 1124, 1422
DpnI	GATC	5 cut(s)	391, 404, 459, 536, 1320
DpnII	GATC	5 cut(s)	389, 402, 457, 534, 1318
DraI	TTTAAA	2 cut(s)	1362, 1458
DraIII	CACNNNGTG	1 cut(s)	1046
DrdI	GACNNNNNNGTC	1 cut(s)	797
DseDI	GACNNNNNNGTC	1 cut(s)	797
EaeI	YGGCCR	1 cut(s)	9
Eam1104I	CTCTTC	2 cut(s)	62, 1499
EarI	CTCTTC	2 cut(s)	62, 1499
Eco24I	GRGCYC	2 cut(s)	337, 1357
Eco47I	GGWCC	1 cut(s)	1561
Eco47III	AGCGCT	1 cut(s)	303
Eco81I	CCTNAGG	1 cut(s)	1422
EcoRII	CCWGG	1 cut(s)	1438
EcoT22I	ATGCAT	1 cut(s)	1591
EcoT38I	GRGCYC	2 cut(s)	337, 1357
FaeI	CATG	4 cut(s)	291, 560, 634, 682
FalI	AAGNNNNNCTT	2 cut(s)	789, 821
FatI	CATG	4 cut(s)	287, 556, 630, 678
FokI	GGATG	8 cut(s)	171, 230, 257, 401, 530, 1118, 1189, 1484
FriOI	GRGCYC	2 cut(s)	337, 1357
FspBI	CTAG	6 cut(s)	672, 731, 959, 995, 1119, 1482
GlaI	GCGC	2 cut(s)	303, 1038
GsuI	CTGGAG	2 cut(s)	1060, 1222
HaeII	RGCGCY	2 cut(s)	305, 1040
HaeIII	GGCC	3 cut(s)	11, 345, 685
HapII	CCGG	3 cut(s)	85, 686, 1514
HgaI	GACGC	1 cut(s)	17
HhaI	GCGC	2 cut(s)	304, 1039
Hin1II	CATG	4 cut(s)	291, 560, 634, 682
Hin6I	GCGC	2 cut(s)	302, 1037
HinP1I	GCGC	2 cut(s)	302, 1037
HindIII	AAGCTT	2 cut(s)	58, 195
HinfI	GANTC	9 cut(s)	77, 109, 149, 155, 167, 727, 735, 1307, 1510
HpaII	CCGG	3 cut(s)	85, 686, 1514
HphI	GGTGA	3 cut(s)	806, 1234, 1529
Hpy166II	GTNNAC	2 cut(s)	1450, 1520
Hpy188III	TCNNGA	9 cut(s)	106, 440, 638, 652, 679, 806, 863, 1294, 1350
Hpy8I	GTNNAC	2 cut(s)	1450, 1520
HpyAV	CCTTC	4 cut(s)	803, 1186, 1327, 1625
HpyCH4III	ACNGT	6 cut(s)	466, 754, 794, 1093, 1375, 1447
HpyCH4IV	ACGT	2 cut(s)	573, 774
HpyF3I	CTNAG	5 cut(s)	203, 209, 397, 1124, 1422
HpySE526I	ACGT	2 cut(s)	573, 774
Hsp92II	CATG	4 cut(s)	291, 560, 634, 682
HspAI	GCGC	2 cut(s)	302, 1037
Kzo9I	GATC	5 cut(s)	389, 402, 457, 534, 1318
LmnI	GCTCC	4 cut(s)	975, 991, 1079, 1296
Lsp1109I	GCAGC	9 cut(s)	595, 623, 829, 937, 1046, 1058, 1327, 1330, 1393
LweI	GCATC	5 cut(s)	423, 506, 1089, 1142, 1462
MabI	ACCWGGT	1 cut(s)	1438
MaeI	CTAG	6 cut(s)	672, 731, 959, 995, 1119, 1482
MaeII	ACGT	2 cut(s)	573, 774
MaeIII	GTNAC	5 cut(s)	156, 520, 788, 799, 1222
MalI	GATC	5 cut(s)	391, 404, 459, 536, 1320
MboI	GATC	5 cut(s)	389, 402, 457, 534, 1318
MboII	GAAGA	8 cut(s)	79, 260, 479, 774, 1154, 1208, 1239, 1516
MflI	RGATCY	1 cut(s)	534
MhlI	GDGCHC	2 cut(s)	337, 1357
MlsI	TGGCCA	1 cut(s)	11
MluCI	AATT	6 cut(s)	40, 702, 1158, 1289, 1636, 1645
MluNI	TGGCCA	1 cut(s)	11
MlyI	GAGTC	1 cut(s)	164
MmeI	TCCRAC	2 cut(s)	442, 1006
Mox20I	TGGCCA	1 cut(s)	11
Mph1103I	ATGCAT	1 cut(s)	1591
MroXI	GAANNNNTTC	2 cut(s)	1161, 1524
MscI	TGGCCA	1 cut(s)	11
MseI	TTAA	8 cut(s)	909, 914, 1361, 1457, 1553, 1592, 1644, 1648
MslI	CAYNNNNRTG	2 cut(s)	1569, 1584
Msp20I	TGGCCA	1 cut(s)	11
MspA1I	CMGCKG	1 cut(s)	950
MspI	CCGG	3 cut(s)	85, 686, 1514
MspR9I	CCNGG	3 cut(s)	687, 1440, 1515
Mva1269I	GAATGC	3 cut(s)	16, 435, 528
MvaI	CCWGG	1 cut(s)	1440
NciI	CCSGG	2 cut(s)	687, 1515
NdeII	GATC	5 cut(s)	389, 402, 457, 534, 1318
NlaIII	CATG	4 cut(s)	291, 560, 634, 682
NmuCI	GTSAC	4 cut(s)	156, 788, 799, 1222
NsiI	ATGCAT	1 cut(s)	1591
NspI	RCATGY	1 cut(s)	634
OliI	CACNNNNGTG	1 cut(s)	1569
PacI	TTAATTAA	1 cut(s)	1648
PaeI	GCATGC	1 cut(s)	634
PagI	TCATGA	1 cut(s)	678
PaqCI	CACCTGC	1 cut(s)	318
PctI	GAATGC	3 cut(s)	16, 435, 528
PdmI	GAANNNNTTC	2 cut(s)	1161, 1524
PfeI	GAWTC	8 cut(s)	77, 109, 149, 167, 727, 735, 1307, 1510
PleI	GAGTC	1 cut(s)	163
PpsI	GAGTC	1 cut(s)	163
PsiI	TTATAA	1 cut(s)	597
Psp6I	CCWGG	1 cut(s)	1438
PspGI	CCWGG	1 cut(s)	1438
PspPI	GGNCC	2 cut(s)	344, 1561
PstI	CTGCAG	3 cut(s)	321, 613, 838
PstNI	CAGNNNCTG	2 cut(s)	842, 1445
PsuI	RGATCY	1 cut(s)	534
PvuII	CAGCTG	1 cut(s)	950
RsaI	GTAC	5 cut(s)	385, 555, 1437, 1444, 1602
RsaNI	GTAC	5 cut(s)	384, 554, 1436, 1443, 1601
RseI	CAYNNNNRTG	2 cut(s)	1569, 1584
SaqAI	TTAA	8 cut(s)	909, 914, 1361, 1457, 1553, 1592, 1644, 1648
Sau3AI	GATC	5 cut(s)	389, 402, 457, 534, 1318
Sau96I	GGNCC	2 cut(s)	344, 1561
ScaI	AGTACT	1 cut(s)	385
SchI	GAGTC	1 cut(s)	164
ScrFI	CCNGG	3 cut(s)	687, 1440, 1515
SduI	GDGCHC	2 cut(s)	337, 1357
SexAI	ACCWGGT	1 cut(s)	1438
SfaNI	GCATC	5 cut(s)	423, 506, 1089, 1142, 1462
SfcI	CTRYAG	4 cut(s)	317, 609, 834, 945
SinI	GGWCC	1 cut(s)	1561
SmiMI	CAYNNNNRTG	2 cut(s)	1569, 1584
SmlI	CTYRAG	2 cut(s)	161, 305
SmoI	CTYRAG	2 cut(s)	161, 305
SphI	GCATGC	1 cut(s)	634
Sse9I	AATT	6 cut(s)	40, 702, 1158, 1289, 1636, 1645
SsiI	CCGC	2 cut(s)	1265, 1337
SspI	AATATT	1 cut(s)	668
SspMI	CTAG	6 cut(s)	672, 731, 959, 995, 1119, 1482
StyD4I	CCNGG	3 cut(s)	685, 1438, 1513
TaaI	ACNGT	6 cut(s)	466, 754, 794, 1093, 1375, 1447
TaiI	ACGT	2 cut(s)	576, 777
TaqI	TCGA	2 cut(s)	388, 637
TasI	AATT	6 cut(s)	40, 702, 1158, 1289, 1636, 1645
TatI	WGTACW	2 cut(s)	383, 1600
TauI	GCSGC	2 cut(s)	1268, 1340
TfiI	GAWTC	8 cut(s)	77, 109, 149, 167, 727, 735, 1307, 1510
Tru1I	TTAA	8 cut(s)	909, 914, 1361, 1457, 1553, 1592, 1644, 1648
Tru9I	TTAA	8 cut(s)	909, 914, 1361, 1457, 1553, 1592, 1644, 1648
TscAI	CASTG	3 cut(s)	27, 469, 838
TseFI	GTSAC	4 cut(s)	156, 788, 799, 1222
TseI	GCWGC	9 cut(s)	608, 611, 842, 950, 1034, 1071, 1340, 1343, 1406
Tsp45I	GTSAC	4 cut(s)	156, 788, 799, 1222
TspDTI	ATGAA	6 cut(s)	80, 173, 261, 276, 1170, 1644
TspGWI	ACGGA	2 cut(s)	728, 962
TspRI	CASTG	3 cut(s)	27, 469, 838
VpaK11BI	GGWCC	1 cut(s)	1561
XapI	RAATTY	2 cut(s)	1158, 1289
XceI	RCATGY	1 cut(s)	634
XmnI	GAANNNNTTC	2 cut(s)	1161, 1524
XspI	CTAG	6 cut(s)	672, 731, 959, 995, 1119, 1482
ZrmI	AGTACT	1 cut(s)	385
Zsp2I	ATGCAT	1 cut(s)	1591

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.