Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0007152

Physical Location & Seq

Genomic Coordinates Reverse (-)

32838423 .. 32845256

Gene Structure 6834 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1201000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2901 bp

ATGGAGAGCCCGCAGCCCCAAAAGCCATGGAAGGTGGAGTACGCCAAGTCGTCGAGATCGTCGTGCAAGACCTGCAAATCCCCCATTGAAAAGGAGACGCTCCGCCTCGGCAAGATGGTCCAGGCCACCCAATTCGACGGCTTCATGCCTATGTGGAACCATGCTCGTTGTATAATGAAGAAATCGAAGCAGATAAAATCAACTGATGAGGTTGAAGGCCTGGAGCTCCTTCGTTGGGAAGATCAGCAGAAAATTAGAAGTTATGTACAAAGTGGTGGACCCCTGGATACAACACTGGCAACAAGCAAGTTCGCTACTAAAGAATGTGGTATTTCAGTTTCACCAACTTCTCGCGCTACTTGCAAGCAATGCAGCCAAAAGATTTTGAAAGCGGAGGTCCGTATATCATCCAAGCCTGAAGGTCAAGGGCCTAGGGGCCTGGCATGGCACCATGCCAACTGCTTCATGGAATTATCACCATCCATCGAAGTGGAGAAGTTGTCTGGATGGGAGACCCTCCCAGTTTCTGATCAGGAAGTTGTACGTGCCTTGGTTAAGAAGGTTCCTTCTAATGCTGGCACAAAACGAAGAAAAGATGCTGGTGATGATCAGAAGTTAAAAGTTGCTAGGTCTGAAGGAGATGTGTCAATGAGCAGGGATGTATCTGTGAGGAAGGCTACTGATGTGGGGAGTAAACTGGAGGCCCAAACTAAAGAGCTGTGGGCACTGAAAGATGATCTTAAAAAGCATGTGACGAATGCAGAGATGCGTGAAATGCTTGAAGTCAATAATCAAGATTCAACAGGATCAGAGCTTGATTTGCGTGAGCGCTGTGCTGATGGAATGATGTTTGGAGCACTTAAGAACTGCCCACTCTGCTCTGGTTTTCTTCGATATTCTGGAGGTATGTACCGGTGCCAAGGCTACCTATCGGCATGGAGTAAGTGTTCTTACTCTACTCAGGAACCTGAACGTCTTGAAGGGAAGTGGAAAATCTCAAAAGATAAGGATAATCATTATCTTAATAAGTGGTTTAAATCACAAAAGCTTGCAAAACCTGTGCGGATACTGCCTCCATTAACACCTAGCAAGCCTTCTGGAAGTCAAGGCCAATCTCAGTCATCTAACAGTACAAGCTTGGCGGATTTAAAAGTTGTTTTTCGTGGATTACCAAAAGAATCAACAGAAGAATGGTGTAGAAAAATTGAGGGTGTAGCTGGAGTATTTCATTCAAAGATCAAAAAAGATACTAATTGCTTAGTTGTGAGTGGGGCATTTGATGATGATGCTGAGATGAGAAAAGCAAGGAGGATGAAGTTACCAATCGTTAGGGAAGACTATCTGGTTGATTGCTTTAAAAGACAGAAGAAGCTTCCATTTGATATTTACAAAGTCGAAGCTCTCCGTGAATCCTCTAGCATGATCACTGTCAAAGTGAAAGGTCGAAGTGCTGTTCATGAATCTTCTGGTCTGCAAGATACAGGACATATTCTTGAGGATGGGAAGAGCATCTACAACACAACTTTAAGCATGTCTGATTTGTCAACTGGTGTTAACAGTTACTACATTCTCCAAATTGTACAAGATGATAAGAGTTCAGACTGCCATGTGTTCCGTAAATGGGGAAGAGTGGGGAATGATAAAATTGGAGGAAGCAAACTGGAAGATATGTCGAAATCAGATGCGATCCATGAATTTAAACGTTTGTTCCTTGAGAAGACTGGGAATCCGTGGGAAGCATGGGAGCAAAAACAAAATTTTCAGAAGCAAGCTGGAAGATTCTTTCCATTGGATATTGATTATGGAGTTAATAAACAGGTTTCCAAGAAAAACCAGAACAATGCAGGCAGCAAATTGGCTCCTCAGTTAGCAGAATTGATGAAGATGCTTTTCAATGTTGAAACATACAGAGCTGCCATGATGGAATTTGAGATAAATATGTCAGAAATGCCGCTTGGAAAACTGAGTAAAGCCAACATCCAAAAGGGGTTTGAGGCATTAACGGAAATACAGAATTTATTGAATAATAATGGTCATGCTCCTTCCACAAAAGAAAGCTTGATTATAGATGCAAGCAACCGTTTTTTCACTGTGATCCCTTCCATACACCCACGGGTTATCAGGGATGAGGATGATTTTAAGTCAAAGGTGAAAATGTTAGAAGCTCTCCAGGACATTGAAATTGCTTCCAGATTAGTTGGTTTTGATGCTGATAGTGATGATTCCCTTGATGAGAAGTATAAGAAGCTCCGGTGTGATATTGATCCAATCCCTCATGATAGTGAAGATTATCAGTTGATTGAAAAATATCTTCTTACTACTCATGCCCCTACACATACGGATTGGAGTCTTGAACTCGAAGAAGTTTTTGCACTTGAAAGGGAAGGCGAATTCAATAAATTTGCTCCCTATCGGAAAAAGCTTAAGAACAAAATGCTTCTTTGGCATGGTTCTCGGTTTACAAATTTCGTGGGTATACTTAGCCAAGGACTGAGAATAGCTCCTCCTGAAGCTCCAGCTACTGGTTATATGTTTGGCAAGGGAATTTACTTTGCTGACCTTGTCAGCAAGAGTGCACAGTATTGCTATACAGATAAGAAAAATCCTGTTGGCCTCATGCTTTTAAGTGAAGTTGCATTGGGGGAGGTCTACGAGCTCAAGAAGGCTGCATATATGGACAAACCTCCAAAGGGAAAGCACTCTACAATGGGGCTTGGCAAGAAAATACCTCAGGAGTCGGAGTATGTAAAATGGAAGGACGATGTCATCGTGCCTTGTGGCAAACCAGTGCCATCAAATGTCAAAGCTTCTGAGCTCATGTATAACGAGTACATCGTTTATGATACAGCTCAAGTGAAGATGCAATACCTGTTGAAGGTGAGGTTTCATCACAAGAGGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0003824 GO:0003909 GO:0003910 GO:0003950 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006266 GO:0006271 GO:0006273 GO:0006281 GO:0006464 GO:0006471 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006979 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009719 GO:0009725 GO:0009737 GO:0009987 GO:0010033 GO:0016070 GO:0016740 GO:0016757 GO:0016763 GO:0016874 GO:0016886 GO:0019538 GO:0022616 GO:0033554 GO:0033993 GO:0034641 GO:0034645 GO:0036211 GO:0042221 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0046483 GO:0050896 GO:0051103 GO:0051716 GO:0071704 GO:0090304 GO:0097305 GO:0140097 GO:1901360 GO:1901564 GO:1901576 GO:1901700

KEGG Pathways

Metabolic & Signaling

ko03410 ko04210 ko04212 ko04214 ko04217 map03410 map04210 map04212 map04214 map04217

Pfam Domains

Protein Families

BRCT PADR1 PARP PARP_reg WGR zf-PARP

Protein Analysis

967

Amino Acids

109.47

Weight (kDa)

9.01

Isoelectric Point (pI)

43.71

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
zf-PARP	PF00645	13 - 88	2.8e-22	Poly(ADP-ribose) polymerase and DNA-Ligase Zn-finger region
zf-PARP	PF00645	114 - 184	8.9e-12	Poly(ADP-ribose) polymerase and DNA-Ligase Zn-finger region
PADR1_N	PF21728	231 - 285	2.6e-21	PADR1, N-terminal helical domain
Zn_ribbon_PADR1	PF08063	286 - 325	3.9e-16	PADR1 domain, zinc ribbon fold
BRCT	PF00533	378 - 452	3.2e-09	BRCA1 C Terminus (BRCT) domain
WGR	PF05406	505 - 583	2.1e-16	WGR domain
PARP_reg	PF02877	617 - 747	2.9e-37	Poly(ADP-ribose) polymerase, regulatory domain
PARP	PF00644	761 - 962	4.5e-82	Poly(ADP-ribose) polymerase catalytic domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0007152)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G31320
fragaria_vesca	FvH4_2g21980 FvH4_2g21980 FvH4_2g21980
malus_domestica	MD05G1201000.v1.1 MD10G1187800.v1.1
prunus_persica	Prupe.8G227600_v2.0.a1 Prupe.8G227600_v2.0.a1
pyrus_communis	pycom05g18780 pycom10g16230
rosa_chinensis	RchiOBHm_Chr6g0288861
rosa_laevigata	RLG00000012360
rosa_multiflora	Rmu_sc0005314.1_g000009
rosa_roxburghii	Rroxscaffold_7G00179830
rosa_rugosa	Rorug06G0198600 Rorug06G0198600 Rorug06G0198600 Rorug06G0198700 Rorug06G0198800 Rorug06G0198900 Rorug06G0198900
rosa_samantha	Rh6AG310000 Rh6BG315900 Rh6CG323400
rosa_wichuraiana	Rw6G026810

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	80
AccB1I	GGYRCC	2 cut(s)	447, 915
AccB7I	CCANNNNNTGG	1 cut(s)	2522
AccI	GTMKAC	2 cut(s)	2476, 2649
AccII	CGCG	1 cut(s)	354
AciI	CCGC	6 cut(s)	11, 103, 392, 1063, 1142, 1952
AclI	AACGTT	1 cut(s)	1702
AclWI	GGATC	4 cut(s)	814, 1681, 2089, 2258
AcsI	RAATTY	8 cut(s)	1694, 1756, 1925, 2014, 2390, 2399, 2464, 2544
AcuI	CTGAAG	3 cut(s)	438, 654, 2529
AfaI	GTAC	7 cut(s)	41, 267, 543, 911, 1132, 1581, 2831
AfeI	AGCGCT	1 cut(s)	830
AfiI	CCNNNNNNNGG	5 cut(s)	235, 1621, 2522, 2690, 2875
AflII	CTTAAG	2 cut(s)	860, 2423
AgeI	ACCGGT	1 cut(s)	912
AjnI	CCWGG	5 cut(s)	120, 219, 282, 438, 2169
AjuI	GAANNNNNNNTTGG	2 cut(s)	1938, 1970
AloI	GAACNNNNNNTCC	2 cut(s)	931, 963
Alw21I	GWGCWC	5 cut(s)	228, 859, 2578, 2658, 2817
Alw26I	GTCTC	2 cut(s)	89, 506
Alw44I	GTGCAC	1 cut(s)	2574
AlwI	GGATC	4 cut(s)	814, 1681, 2089, 2258
AlwNI	CAGNNNCTG	2 cut(s)	527, 2522
Aor51HI	AGCGCT	1 cut(s)	830
AoxI	GGCC	7 cut(s)	123, 217, 428, 436, 702, 1108, 2611
ApaLI	GTGCAC	1 cut(s)	2574
ApeKI	GCWGC	5 cut(s)	13, 372, 1848, 1913, 2666
ApoI	RAATTY	8 cut(s)	1694, 1756, 1925, 2014, 2390, 2399, 2464, 2544
AsiGI	ACCGGT	1 cut(s)	912
AspA2I	CCTAGG	1 cut(s)	431
AspLEI	GCGC	2 cut(s)	356, 831
AspS9I	GGNCC	6 cut(s)	118, 278, 397, 428, 436, 703
AsuHPI	GGTGA	5 cut(s)	333, 468, 614, 2161, 2890
AvaII	GGWCC	3 cut(s)	118, 278, 397
AvrII	CCTAGG	1 cut(s)	431
AxyI	CCTNAGG	1 cut(s)	2730
BaeGI	GKGCMC	2 cut(s)	727, 2578
BanI	GGYRCC	2 cut(s)	447, 915
BanII	GRGCYC	4 cut(s)	11, 228, 2658, 2817
BarI	GAAGNNNNNNTAC	6 cut(s)	23, 55, 1496, 1528, 2849, 2881
BbsI	GAAGAC	2 cut(s)	1341, 1724
Bbv12I	GWGCWC	5 cut(s)	228, 859, 2578, 2658, 2817
BbvI	GCAGC	5 cut(s)	25, 384, 1860, 1900, 2653
BccI	CCATC	8 cut(s)	109, 487, 491, 501, 833, 1493, 1915, 2800
BceAI	ACGGC	1 cut(s)	154
BcgI	CGANNNNNNTGC	2 cut(s)	2435, 2469
BciT130I	CCWGG	5 cut(s)	122, 221, 284, 440, 2171
BciVI	GTATCC	2 cut(s)	280, 1059
BclI	TGATCA	3 cut(s)	529, 607, 1422
BcoDI	GTCTC	2 cut(s)	89, 506
BfaI	CTAG	4 cut(s)	432, 627, 1086, 1416
BfoI	RGCGCY	1 cut(s)	832
BfrI	CTTAAG	2 cut(s)	860, 2423
BfuAI	ACCTGC	1 cut(s)	80
BfuI	GTATCC	2 cut(s)	280, 1059
BisI	GCNGC	6 cut(s)	14, 373, 1849, 1914, 1952, 2667
BlnI	CCTAGG	1 cut(s)	431
BlsI	GCNGC	6 cut(s)	15, 374, 1850, 1915, 1953, 2668
Bme1390I	CCNGG	5 cut(s)	122, 221, 284, 440, 2171
Bme18I	GGWCC	3 cut(s)	118, 278, 397
BmgT120I	GGNCC	6 cut(s)	118, 278, 397, 428, 436, 703
BmiI	GGNNCC	8 cut(s)	158, 280, 437, 449, 564, 917, 966, 1860
BmrFI	CCNGG	5 cut(s)	122, 221, 284, 440, 2171
BmrI	ACTGGG	2 cut(s)	515, 1731
BmsI	GCATC	9 cut(s)	586, 756, 1276, 1518, 1672, 1875, 2059, 2197, 2850
BmuI	ACTGGG	2 cut(s)	515, 1731
BpiI	GAAGAC	2 cut(s)	1341, 1724
BplI	GAGNNNNNCTC	2 cut(s)	2485, 2517
BpmI	CTGGAG	6 cut(s)	242, 719, 921, 1239, 2153, 2499
BpuEI	CTTGAG	4 cut(s)	1514, 1733, 2642, 2835
BsaAI	YACGTR	1 cut(s)	545
BsaBI	GATNNNNATC	1 cut(s)	2262
BsaI	GGTCTC	1 cut(s)	506
BsaJI	CCNNGG	9 cut(s)	26, 106, 282, 431, 549, 919, 1730, 2111, 2485
BsaWI	WCCGGW	2 cut(s)	912, 2250
BsaXI	ACNNNNNCTCC	6 cut(s)	485, 515, 931, 961, 2726, 2756
Bsc4I	CCNNNNNNNGG	5 cut(s)	235, 1621, 2522, 2690, 2875
Bse118I	RCCGGY	1 cut(s)	912
Bse1I	ACTGG	8 cut(s)	300, 521, 702, 1552, 1665, 1726, 2527, 2786
Bse21I	CCTNAGG	1 cut(s)	2730
Bse3DI	GCAATG	1 cut(s)	374
Bse8I	GATNNNNATC	1 cut(s)	2262
BseBI	CCWGG	5 cut(s)	122, 221, 284, 440, 2171
BseDI	CCNNGG	9 cut(s)	26, 106, 282, 431, 549, 919, 1730, 2111, 2485
BseGI	GGATG	9 cut(s)	407, 479, 512, 664, 1317, 1504, 1977, 2131, 2137
BseJI	GATNNNNATC	1 cut(s)	2262
BseLI	CCNNNNNNNGG	5 cut(s)	235, 1621, 2522, 2690, 2875
BseMI	GCAATG	1 cut(s)	374
BseMII	CTCAG	8 cut(s)	974, 1130, 1281, 1877, 1955, 2483, 2744, 2802
BseNI	ACTGG	8 cut(s)	300, 521, 702, 1552, 1665, 1726, 2527, 2786
BseRI	GAGGAG	2 cut(s)	1851, 2493
BseSI	GKGCMC	2 cut(s)	727, 2578
BseXI	GCAGC	5 cut(s)	25, 384, 1860, 1900, 2653
Bsh1236I	CGCG	1 cut(s)	354
BshFI	GGCC	7 cut(s)	125, 219, 430, 438, 704, 1110, 2613
BshNI	GGYRCC	2 cut(s)	447, 915
BshTI	ACCGGT	1 cut(s)	912
BsiHKAI	GWGCWC	5 cut(s)	228, 859, 2578, 2658, 2817
BsiSI	CCGG	2 cut(s)	913, 2251
BslI	CCNNNNNNNGG	5 cut(s)	235, 1621, 2522, 2690, 2875
BsmAI	GTCTC	2 cut(s)	89, 506
BsmBI	CGTCTC	1 cut(s)	89
BsmI	GAATGC	1 cut(s)	763
BsnI	GGCC	7 cut(s)	125, 219, 430, 438, 704, 1110, 2613
Bso31I	GGTCTC	1 cut(s)	506
Bsp1286I	GDGCHC	7 cut(s)	11, 228, 727, 859, 2578, 2658, 2817
Bsp1407I	TGTACA	2 cut(s)	265, 1579
Bsp19I	CCATGG	1 cut(s)	26
BspACI	CCGC	6 cut(s)	11, 103, 392, 1063, 1142, 1952
BspANI	GGCC	7 cut(s)	125, 219, 430, 438, 704, 1110, 2613
BspCNI	CTCAG	8 cut(s)	973, 1129, 1282, 1876, 1956, 2484, 2743, 2803
BspFNI	CGCG	1 cut(s)	354
BspHI	TCATGA	2 cut(s)	1456, 2275
BspLI	GGNNCC	8 cut(s)	158, 280, 437, 449, 564, 917, 966, 1860
BspMI	ACCTGC	1 cut(s)	80
BspPI	GGATC	4 cut(s)	814, 1681, 2089, 2258
BspQI	GCTCTTC	1 cut(s)	1499
BspT107I	GGYRCC	2 cut(s)	447, 915
BspTI	CTTAAG	2 cut(s)	860, 2423
BspTNI	GGTCTC	1 cut(s)	506
BsrDI	GCAATG	1 cut(s)	374
BsrFI	RCCGGY	1 cut(s)	912
BsrGI	TGTACA	2 cut(s)	265, 1579
BsrI	ACTGG	8 cut(s)	300, 521, 702, 1552, 1665, 1726, 2527, 2786
BssAI	RCCGGY	1 cut(s)	912
BssECI	CCNNGG	9 cut(s)	26, 106, 282, 431, 549, 919, 1730, 2111, 2485
BssNAI	GTATAC	1 cut(s)	2477
BssT1I	CCWWGG	5 cut(s)	26, 431, 549, 919, 2485
Bst1107I	GTATAC	1 cut(s)	2477
Bst2UI	CCWGG	5 cut(s)	122, 221, 284, 440, 2171
Bst4CI	ACNGT	6 cut(s)	1130, 1429, 1559, 2081, 2092, 2580
Bst6I	CTCTTC	2 cut(s)	1499, 1621
BstAFI	CTTAAG	2 cut(s)	860, 2423
BstAPI	GCANNNNNTGC	2 cut(s)	72, 369
BstAUI	TGTACA	2 cut(s)	265, 1579
BstBAI	YACGTR	1 cut(s)	545
BstC8I	GCNNGC	8 cut(s)	11, 365, 577, 1050, 1091, 1770, 1846, 2074
BstDSI	CCRYGG	3 cut(s)	26, 1730, 2111
BstENI	CCTNNNNNAGG	1 cut(s)	2873
BstF5I	GGATG	9 cut(s)	407, 479, 512, 664, 1317, 1504, 1977, 2131, 2137
BstFNI	CGCG	1 cut(s)	354
BstH2I	RGCGCY	1 cut(s)	832
BstHHI	GCGC	2 cut(s)	356, 831
BstMAI	GTCTC	2 cut(s)	89, 506
BstMWI	GCNNNNNNNGC	9 cut(s)	22, 72, 360, 369, 775, 820, 876, 1069, 2443
BstNI	CCWGG	5 cut(s)	122, 221, 284, 440, 2171
BstNSI	RCATGY	2 cut(s)	752, 1534
BstSCI	CCNGG	5 cut(s)	120, 219, 282, 438, 2169
BstSLI	GKGCMC	2 cut(s)	727, 2578
BstUI	CGCG	1 cut(s)	354
BstV1I	GCAGC	5 cut(s)	25, 384, 1860, 1900, 2653
BstV2I	GAAGAC	2 cut(s)	1341, 1724
BstXI	CCANNNNNNTGG	1 cut(s)	490
BstZ17I	GTATAC	1 cut(s)	2477
Bsu36I	CCTNAGG	1 cut(s)	2730
BsuI	GTATCC	2 cut(s)	280, 1059
BsuRI	GGCC	7 cut(s)	125, 219, 430, 438, 704, 1110, 2613
BtgI	CCRYGG	3 cut(s)	26, 1730, 2111
BtsCI	GGATG	9 cut(s)	407, 479, 512, 664, 1317, 1504, 1977, 2131, 2137
BtsIMutI	CAGTG	5 cut(s)	293, 725, 1425, 2088, 2793
BveI	ACCTGC	1 cut(s)	80
Cac8I	GCNNGC	8 cut(s)	11, 365, 577, 1050, 1091, 1770, 1846, 2074
CaiI	CAGNNNCTG	2 cut(s)	527, 2522
CciI	TCATGA	2 cut(s)	1456, 2275
CfoI	GCGC	2 cut(s)	356, 831
Cfr10I	RCCGGY	1 cut(s)	912
Cfr13I	GGNCC	6 cut(s)	118, 278, 397, 428, 436, 703
CseI	GACGC	1 cut(s)	106
Csp6I	GTAC	7 cut(s)	40, 266, 542, 910, 1131, 1580, 2830
CspAI	ACCGGT	1 cut(s)	912
CspCI	CAANNNNNGTGG	2 cut(s)	2451, 2486
CviQI	GTAC	7 cut(s)	40, 266, 542, 910, 1131, 1580, 2830
DraI	TTTAAA	4 cut(s)	1036, 1149, 1357, 1699
Eam1104I	CTCTTC	2 cut(s)	1499, 1621
EarI	CTCTTC	2 cut(s)	1499, 1621
EciI	GGCGGA	2 cut(s)	92, 1157
Ecl136II	GAGCTC	3 cut(s)	226, 2656, 2815
Eco130I	CCWWGG	5 cut(s)	26, 431, 549, 919, 2485
Eco147I	AGGCCT	1 cut(s)	219
Eco24I	GRGCYC	4 cut(s)	11, 228, 2658, 2817
Eco31I	GGTCTC	1 cut(s)	506
Eco47I	GGWCC	3 cut(s)	118, 278, 397
Eco47III	AGCGCT	1 cut(s)	830
Eco53kI	GAGCTC	3 cut(s)	226, 2656, 2815
Eco57I	CTGAAG	3 cut(s)	438, 654, 2529
Eco81I	CCTNAGG	1 cut(s)	2730
EcoICRI	GAGCTC	3 cut(s)	226, 2656, 2815
EcoNI	CCTNNNNNAGG	1 cut(s)	2873
EcoO109I	RGGNCCY	2 cut(s)	428, 436
EcoRI	GAATTC	1 cut(s)	2390
EcoRII	CCWGG	5 cut(s)	120, 219, 282, 438, 2169
EcoT14I	CCWWGG	5 cut(s)	26, 431, 549, 919, 2485
EcoT38I	GRGCYC	4 cut(s)	11, 228, 2658, 2817
ErhI	CCWWGG	5 cut(s)	26, 431, 549, 919, 2485
Esp3I	CGTCTC	1 cut(s)	89
FalI	AAGNNNNNCTT	2 cut(s)	723, 755
FauI	CCCGC	1 cut(s)	18
FbaI	TGATCA	3 cut(s)	529, 607, 1422
FblI	GTMKAC	2 cut(s)	2476, 2649
Fnu4HI	GCNGC	6 cut(s)	14, 373, 1849, 1914, 1952, 2667
FokI	GGATG	9 cut(s)	394, 466, 519, 671, 1324, 1511, 1964, 2138, 2144
FriOI	GRGCYC	4 cut(s)	11, 228, 2658, 2817
Fsp4HI	GCNGC	6 cut(s)	14, 373, 1849, 1914, 1952, 2667
FspBI	CTAG	4 cut(s)	432, 627, 1086, 1416
GlaI	GCGC	2 cut(s)	355, 830
GluI	GCNGC	6 cut(s)	14, 373, 1849, 1914, 1952, 2667
GsuI	CTGGAG	6 cut(s)	242, 719, 921, 1239, 2153, 2499
HaeII	RGCGCY	1 cut(s)	832
HaeIII	GGCC	7 cut(s)	125, 219, 430, 438, 704, 1110, 2613
HapII	CCGG	2 cut(s)	913, 2251
HgaI	GACGC	1 cut(s)	106
HhaI	GCGC	2 cut(s)	356, 831
Hin6I	GCGC	2 cut(s)	354, 829
HinP1I	GCGC	2 cut(s)	354, 829
HincII	GTYRAC	2 cut(s)	1545, 1555
HindII	GTYRAC	2 cut(s)	1545, 1555
HindIII	AAGCTT	6 cut(s)	1046, 1135, 1370, 2056, 2420, 2805
HinfI	GANTC	9 cut(s)	797, 1178, 1409, 1460, 1726, 1779, 2222, 2347, 2735
HpaI	GTTAAC	1 cut(s)	1555
HpaII	CCGG	2 cut(s)	913, 2251
HphI	GGTGA	5 cut(s)	333, 468, 614, 2161, 2890
Hpy166II	GTNNAC	8 cut(s)	278, 695, 1545, 1555, 2460, 2477, 2576, 2650
Hpy8I	GTNNAC	8 cut(s)	278, 695, 1545, 1555, 2460, 2477, 2576, 2650
Hpy99I	CGWCG	3 cut(s)	55, 64, 140
HpyCH4III	ACNGT	6 cut(s)	1130, 1429, 1559, 2081, 2092, 2580
HpyCH4IV	ACGT	3 cut(s)	544, 973, 1702
HpyF10VI	GCNNNNNNNGC	9 cut(s)	22, 72, 360, 369, 775, 820, 876, 1069, 2443
HpySE526I	ACGT	3 cut(s)	544, 973, 1702
HspAI	GCGC	2 cut(s)	354, 829
Ksp22I	TGATCA	3 cut(s)	529, 607, 1422
KspAI	GTTAAC	1 cut(s)	1555
LguI	GCTCTTC	1 cut(s)	1499
Lsp1109I	GCAGC	5 cut(s)	25, 384, 1860, 1900, 2653
LweI	GCATC	9 cut(s)	586, 756, 1276, 1518, 1672, 1875, 2059, 2197, 2850
MaeI	CTAG	4 cut(s)	432, 627, 1086, 1416
MaeII	ACGT	3 cut(s)	544, 973, 1702
MaeIII	GTNAC	3 cut(s)	751, 1317, 1559
MhlI	GDGCHC	7 cut(s)	11, 228, 727, 859, 2578, 2658, 2817
MlyI	GAGTC	2 cut(s)	2356, 2744
MmeI	TCCRAC	1 cut(s)	2718
MslI	CAYNNNNRTG	3 cut(s)	149, 488, 2280
MspCI	CTTAAG	2 cut(s)	860, 2423
MspI	CCGG	2 cut(s)	913, 2251
MspR9I	CCNGG	5 cut(s)	122, 221, 284, 440, 2171
Mva1269I	GAATGC	1 cut(s)	763
MvaI	CCWGG	5 cut(s)	122, 221, 284, 440, 2171
MvnI	CGCG	1 cut(s)	354
MwoI	GCNNNNNNNGC	9 cut(s)	22, 72, 360, 369, 775, 820, 876, 1069, 2443
NcoI	CCATGG	1 cut(s)	26
NlaIV	GGNNCC	8 cut(s)	158, 280, 437, 449, 564, 917, 966, 1860
NmeAIII	GCCGAG	1 cut(s)	87
NmuCI	GTSAC	1 cut(s)	751
NspI	RCATGY	2 cut(s)	752, 1534
PagI	TCATGA	2 cut(s)	1456, 2275
PceI	AGGCCT	1 cut(s)	219
PciSI	GCTCTTC	1 cut(s)	1499
PcsI	WCGNNNNNNNCGW	4 cut(s)	56, 59, 2766, 2832
PctI	GAATGC	1 cut(s)	763
PfeI	GAWTC	7 cut(s)	797, 1178, 1409, 1460, 1726, 1779, 2222
PflFI	GACNNNGTC	2 cut(s)	2561, 2762
PflMI	CCANNNNNTGG	1 cut(s)	2522
PfoI	TCCNGGA	1 cut(s)	2169
PinAI	ACCGGT	1 cut(s)	912
PkrI	GCNGC	6 cut(s)	15, 374, 1850, 1915, 1953, 2668
PleI	GAGTC	2 cut(s)	2355, 2743
PpsI	GAGTC	2 cut(s)	2355, 2743
Ppu21I	YACGTR	1 cut(s)	545
Psp124BI	GAGCTC	3 cut(s)	228, 2658, 2817
Psp1406I	AACGTT	1 cut(s)	1702
Psp6I	CCWGG	5 cut(s)	120, 219, 282, 438, 2169
PspGI	CCWGG	5 cut(s)	120, 219, 282, 438, 2169
PspN4I	GGNNCC	8 cut(s)	158, 280, 437, 449, 564, 917, 966, 1860
PspPI	GGNCC	6 cut(s)	118, 278, 397, 428, 436, 703
PstNI	CAGNNNCTG	2 cut(s)	527, 2522
PsyI	GACNNNGTC	2 cut(s)	2561, 2762
RsaI	GTAC	7 cut(s)	41, 267, 543, 911, 1132, 1581, 2831
RsaNI	GTAC	7 cut(s)	40, 266, 542, 910, 1131, 1580, 2830
RseI	CAYNNNNRTG	3 cut(s)	149, 488, 2280
SacI	GAGCTC	3 cut(s)	228, 2658, 2817
SapI	GCTCTTC	1 cut(s)	1499
SatI	GCNGC	6 cut(s)	14, 373, 1849, 1914, 1952, 2667
Sau96I	GGNCC	6 cut(s)	118, 278, 397, 428, 436, 703
SchI	GAGTC	2 cut(s)	2356, 2744
ScrFI	CCNGG	5 cut(s)	122, 221, 284, 440, 2171
SduI	GDGCHC	7 cut(s)	11, 228, 727, 859, 2578, 2658, 2817
SfaNI	GCATC	9 cut(s)	586, 756, 1276, 1518, 1672, 1875, 2059, 2197, 2850
SinI	GGWCC	3 cut(s)	118, 278, 397
SmiMI	CAYNNNNRTG	3 cut(s)	149, 488, 2280
SmlI	CTYRAG	6 cut(s)	860, 1493, 1712, 2423, 2657, 2850
SmoI	CTYRAG	6 cut(s)	860, 1493, 1712, 2423, 2657, 2850
SseBI	AGGCCT	1 cut(s)	219
SsiI	CCGC	6 cut(s)	11, 103, 392, 1063, 1142, 1952
SspMI	CTAG	4 cut(s)	432, 627, 1086, 1416
SstI	GAGCTC	3 cut(s)	228, 2658, 2817
StuI	AGGCCT	1 cut(s)	219
StyD4I	CCNGG	5 cut(s)	120, 219, 282, 438, 2169
StyI	CCWWGG	5 cut(s)	26, 431, 549, 919, 2485
TaaI	ACNGT	6 cut(s)	1130, 1429, 1559, 2081, 2092, 2580
TaiI	ACGT	3 cut(s)	547, 976, 1705
TaqI	TCGA	9 cut(s)	53, 135, 185, 486, 892, 1395, 1444, 1673, 2358
TatI	WGTACW	4 cut(s)	265, 1130, 1579, 2829
TauI	GCSGC	1 cut(s)	1954
TfiI	GAWTC	7 cut(s)	797, 1178, 1409, 1460, 1726, 1779, 2222
TscAI	CASTG	5 cut(s)	300, 732, 1432, 2095, 2793
TseFI	GTSAC	1 cut(s)	751
TseI	GCWGC	5 cut(s)	13, 372, 1848, 1913, 2666
Tsp45I	GTSAC	1 cut(s)	751
TspGWI	ACGGA	6 cut(s)	389, 1394, 1604, 1719, 2018, 2354
TspRI	CASTG	5 cut(s)	300, 732, 1432, 2095, 2793
Tth111I	GACNNNGTC	2 cut(s)	2561, 2762
Van91I	CCANNNNNTGG	1 cut(s)	2522
Vha464I	CTTAAG	2 cut(s)	860, 2423
VneI	GTGCAC	1 cut(s)	2574
VpaK11BI	GGWCC	3 cut(s)	118, 278, 397
XagI	CCTNNNNNAGG	1 cut(s)	2873
XapI	RAATTY	8 cut(s)	1694, 1756, 1925, 2014, 2390, 2399, 2464, 2544
XceI	RCATGY	2 cut(s)	752, 1534
XcmI	CCANNNNNNNNNTGG	1 cut(s)	463
XmaJI	CCTAGG	1 cut(s)	431
XmiI	GTMKAC	2 cut(s)	2476, 2649
XspI	CTAG	4 cut(s)	432, 627, 1086, 1416

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.