Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000006

Orthogroup ID

OG0007152

Physical Location & Seq

Genomic Coordinates Forward (+)

31992067 .. 32000772

Gene Structure 8706 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug06G0198900.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3435 bp

ATGTCGGAGGATAATCAAGAACCGGTTGATGTTGATGCCTCTGCATCTCTTCCTTCATCATCAGCTGAATCATCAGCTGAATCATCAGCTCCTAGGTGGAAGCATGATGTGTTTTTGAGCTTCAGGGGTCCAGACACTCGTAAGAGTATTGCATCCTTTTTATACTATCGACTGCAAAAAAGACAAATCAAAACATTCATGGATGACCAAGACCTTCAAGTAGGGGATGTTATTTCTCCCACTCTCCTAACGGCAATTAAAGAATCAAGGTTTGCAATTATTGTTCTCTCTCAAAACTATGCCTCTTCCACTTGGTGTTTGGAGGAACTTAGAAACATTTGTGAATGCATGGAAGAAGACAGCAATAGAATTCTGCCACTTTTTTATTATGTGGATCCTACTGATGTACGATATCAGAAGAGGAGTTTCAGAGATGCTTTTACTAGGCATGAAACATCAGGGCAACACAGATCAGAGAAGGTGCAGCAGTGGAGAGATGCTTTAAAAAAAGTGGCAAATTTCTCTGGATGGCACACACAGAATTATAAAACTGAAAAAGAACTTTTGGATGCCATTGAGGAATCTGTGTGCAATAAATTTCGACCTACTGGACTTGAGTTTAAATTGTCCATCGGAGGTTTTGAAGCATTTGCAGCAACAAAGCAAGCCATTGATAAGGTCATGAATGCGCTAAAAGATGATGAGGCCACTACCATTGGAGTCTACGGAATGGGGGGCGTTGGAAAGACAACAATGGTGAAATATGTTGGAGCACAAGCCCAAAAAAGTAGGCTTTTTAATCAAGTGATTATGGCTGTCGTATCCCAAAACCCCGACTTGATGAAAATTCAAGAGACATTTGCAGAACTGCTGGGCTTTAAATTGGAGGAGAAGACAGAAATTGGAAGAGCCATTAAATTGAAGGAGAAGATAATGAGAGGAACCGGGATCCTCATAATCTTGGATGACATTTGGAAGAGAATAGACTTTTCAAGCATAGGAATTCCGAGCCACAACGAACTCCAAAGATGCAATTCCAAAGTTCTACTGACCACCAGAAAACCGAGTGTTTGCCGTTCCATGGATTGCCATCCAAACATACATCTCAATATCTTATCACAAGAAGATTCTTGGAGCTTATTTGTGAAGGAAGCAAGGAAGTCTTTTGACAAATCATCCAATCTCTATGATGTAGCACGTAAGGTGGCTAGAGAATGCGCTGGTCTACCAATTGCTTTGATAGCAGTTGCGAGGGCCCTTCGAGATGAAGATTTGGACGGATGGAAAGAAGCTGCTCGACGACTTAAAGAGTCTCAACCTCCCATCCTTGAAGATGAGGGAGATGTGTTCAAATGCATAAAGTTAAGCTATGATTACTTGAAATCTGATGATTCCAAATCATGCTTCTTGCTTTGCTGCCTGTTCCCAGAAGATTATGATATCCCAATTGAATACTTGTTCAAGTATGGAGTTGGGAAAGGGATGTTTCAAGATTCAAACTTGCTAGATGCCCGAGCCACAACATATTTAGTGGTGAAGGCCCTTAAAGATTCTAGCTTGCTTTTGGACGCTAGAGATGACGGATGCGTAAAGATGCATGATGTCATTCGGGATATGGCAATCTCAATTTCGTTATCGGAAGGCCAGCGGGTCTTGGTGAAAGCTGGCTGTGAATTGAAGAATTGGCCACGGATTGATGCACATAAAGGCTACTATGGAATCTCACTAATGGAGAACAAGATTTGCAAGCTGCCCCAAGTGTTGGTATGTCCAAATCTCCAGATTTTATTACTGCAACACAATGCTTCTCTAAATGAGATCCCAAAATCTTTTCTCCAAAGTCAGAATGAATTAAGAGTCTTGGATCTTAGCCACACTGGTATTTCATTACTACCCCAATCAATCAGTTTCTTAACCAACCTTGAAGCTTTGTATTTAGATTACTGCCGGAACATAATTGACATTTCTGTAATCGGAAAACTTCACAAGCTTGAGATTCTTAGTATGAGAGGAACTGCTCTGAAAGAATTGTCAAGAGAAATAGGACAGTTGACCAATCTAAGGATGTTGGATGTCAGTAATGAATATAATCCACGGACATGGAGATTTGATGGACGTATTGCCACAATTCCATCTGAAGTGATATCAAAGTTGCATAAGTTAGAAGAATTGTACATGCAGTGTGGATTTTGGGACTGGGGGAGTAAAATTGATGGAGAAGGAGAAGAAACTAATATTGGTTTTGATGAATTAGCTGGTTTATCATATTTAAGCATTTTGAAAGTTTGCATATCAGATGCAAATTGCATCCCTAAAAGTGTTGAGGTCGAACCGAATTGGGTTTACTTCGATATTAGTATTTGCAGCAACAGTTATGAACAAATGGACCTTAAGAAGTTTCGAGCTAGTGATTCTGGTCATAATTGTAGATCCTTGAGTCTTAATAGTACTGACACAACCATAGGAACATTTCCGGACTGGTTTGTCAACACTGTGGTAAACAATACTGAGAGGCTGCGTTATGAAGACTGCAAAGGGCTAAGTAACATTCTTGTGGAATATGACCGTGGGAGGTTACATGGACTGAAAACTCTCTCTGTAAAAGGTCGCTGTGAGAACTTGAAAGAATTGATGAATGCAATAACATGTGTTCCAGATATGCCTGTGTTTGAGAACTTAGAAGAGTTGTATCTATATTACCTGGATGACCTGAAGCAGTTATGTGTTGGTGAGTTACCACCTGGAACTCTCTGCAGTTTGAAATTATTGGATGTCTTTGGGTGTCGTAACTTGGGTAATGTACTGCTGCCATCAAAATTGTTGCAGAAACTACCAAATCTGGAAAAACTATTCTGTTTGGGTACTAAAGTGGAATATGTGTTTGGATGTGAAGGCTTTGAGCCGGATCAAACAAATCTGAGAGAGATGGATTTATGGGATCTAGACGCAGTAAGAAGCATATGTAATGGTCCTGCTCCACGTGGAATGTTCCAGACACTAAACGATTTGGTCATTGACAATTGCAACTTCCAGGGAAGCCTCTTCACATTTGATGTAGCTCAGTGTCTTTTTCAATTGGAACAACTTTTTGTATCCCAATGCCCTTTCTTGGAAAGACTAATCGAAGCAAGGAGGGAATCATTGAACAACAACAAGACCGTTCTTCCAAATTTGAAGAGCTTATGTTTACAATATCTTCCCATGTTGTACAAGGGAAGTGCTACTATTGATTTTGAGTGTCCTTCATTGGAAATCTTGTGTATCGAGGACTGCCCCCACTTGTCATTTCCATCCTCTGCTTCTGACCACTTCCACAGCAGGAAGCAAGACCTTTTCAATTTGGGTCGGATTACGAAATGGGCTTCCGGTATGTCACTGGGTCATAAACTTAATTCATTTTTCTTTTCTCTTATAGTCTGCTTATATAATTGGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004497 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006464 GO:0006807 GO:0008150 GO:0008152 GO:0008612 GO:0009058 GO:0009987 GO:0010467 GO:0016491 GO:0016705 GO:0018193 GO:0018205 GO:0019135 GO:0019538 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0051604 GO:0055114 GO:0071704 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

HEAT_2 HEAT_PBS

Protein Analysis

1144

Amino Acids

129.95

Weight (kDa)

5.62

Isoelectric Point (pI)

42.95

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
TIR	PF01582	34 - 200	4.9e-50	TIR domain
TIR_2	PF13676	37 - 147	4.3e-16	TIR domain
NB-ARC	PF00931	221 - 386	1.4e-30	NB-ARC domain
LRR_8	PF13855	591 - 650	7.7e-08	Leucine rich repeat
LRR_14	PF23598	593 - 763	5.1e-14	Leucine-rich repeat region
LRR_RPS2	PF23247	893 - 1034	6.9e-19	Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2	PF23247	985 - 1099	6.1e-11	Plant disease resistance protein RPS2-like, leucine-rich repeats

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0007152)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G31320
fragaria_vesca	FvH4_2g21980 FvH4_2g21980 FvH4_2g21980
malus_domestica	MD05G1201000.v1.1 MD10G1187800.v1.1
prunus_persica	Prupe.8G227600_v2.0.a1 Prupe.8G227600_v2.0.a1
pyrus_communis	pycom05g18780 pycom10g16230
rosa_chinensis	RchiOBHm_Chr6g0288861
rosa_laevigata	RLG00000012360
rosa_multiflora	Rmu_sc0005314.1_g000009
rosa_roxburghii	Rroxscaffold_7G00179830
rosa_rugosa	Rorug06G0198600 Rorug06G0198600 Rorug06G0198600 Rorug06G0198700 Rorug06G0198800 Rorug06G0198900 Rorug06G0198900
rosa_samantha	Rh6AG310000 Rh6BG315900 Rh6CG323400
rosa_wichuraiana	Rw6G026810

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	546
AasI	GACNNNNNNGTC	1 cut(s)	2483
AccB7I	CCANNNNNTGG	1 cut(s)	1762
AccI	GTMKAC	2 cut(s)	723, 1225
AccIII	TCCGGA	1 cut(s)	2473
AciI	CCGC	1 cut(s)	1648
AclWI	GGATC	9 cut(s)	389, 402, 943, 956, 1813, 1872, 2424, 2913, 2946
AcoI	YGGCCR	1 cut(s)	1685
AcsI	RAATTY	6 cut(s)	369, 517, 596, 846, 1002, 3169
AcuI	CTGAAG	3 cut(s)	106, 2157, 2732
AcvI	CACGTG	1 cut(s)	2981
AdeI	CACNNNGTG	1 cut(s)	315
AfaI	GTAC	6 cut(s)	408, 2174, 2449, 2802, 2863, 3209
AfiI	CCNNNNNNNGG	4 cut(s)	1081, 1762, 2061, 3109
AflII	CTTAAG	1 cut(s)	2390
AflIII	ACRYGT	1 cut(s)	2645
AgeI	ACCGGT	1 cut(s)	22
AjnI	CCWGG	3 cut(s)	2700, 2740, 3030
AjuI	GAANNNNNNNTTGG	6 cut(s)	1388, 1420, 2220, 2252, 2865, 2897
Alw21I	GWGCWC	1 cut(s)	775
Alw26I	GTCTC	2 cut(s)	848, 1317
AlwI	GGATC	9 cut(s)	389, 402, 943, 956, 1813, 1872, 2424, 2913, 2946
Ama87I	CYCGRG	1 cut(s)	1512
Aor13HI	TCCGGA	1 cut(s)	2473
AoxI	GGCC	5 cut(s)	705, 1254, 1539, 1642, 1685
ApaI	GGGCCC	1 cut(s)	1258
ApeKI	GCWGC	8 cut(s)	484, 653, 1292, 1416, 1750, 2364, 2515, 2806
ApoI	RAATTY	6 cut(s)	369, 517, 596, 846, 1002, 3169
AsiGI	ACCGGT	1 cut(s)	22
AspA2I	CCTAGG	1 cut(s)	92
AspLEI	GCGC	2 cut(s)	691, 1220
AspS9I	GGNCC	6 cut(s)	128, 1254, 1255, 1540, 2386, 2969
AsuC2I	CCSGG	1 cut(s)	946
AsuHPI	GGTGA	4 cut(s)	769, 1546, 1669, 2741
AvaI	CYCGRG	1 cut(s)	1512
AvaII	GGWCC	3 cut(s)	128, 2386, 2969
AvrII	CCTAGG	1 cut(s)	92
BaeGI	GKGCMC	1 cut(s)	1258
BaeI	ACNNNNGTAYC	2 cut(s)	2853, 2886
BalI	TGGCCA	1 cut(s)	1687
BamHI	GGATCC	2 cut(s)	394, 948
BanII	GRGCYC	1 cut(s)	1258
BbrPI	CACGTG	1 cut(s)	2981
BbsI	GAAGAC	3 cut(s)	363, 899, 2532
Bbv12I	GWGCWC	1 cut(s)	775
BbvI	GCAGC	8 cut(s)	496, 665, 1279, 1403, 1737, 2376, 2502, 2793
BceAI	ACGGC	2 cut(s)	267, 1059
BciT130I	CCWGG	3 cut(s)	2702, 2742, 3032
BciVI	GTATCC	2 cut(s)	832, 3103
BcnI	CCSGG	1 cut(s)	946
BcoDI	GTCTC	2 cut(s)	848, 1317
BfaI	CTAG	8 cut(s)	93, 444, 1209, 1505, 1554, 1572, 2406, 2942
BfmI	CTRYAG	1 cut(s)	2752
BfrI	CTTAAG	1 cut(s)	2390
BfuI	GTATCC	2 cut(s)	832, 3103
BisI	GCNGC	8 cut(s)	485, 654, 1293, 1417, 1751, 2365, 2516, 2807
BlnI	CCTAGG	1 cut(s)	92
BlsI	GCNGC	8 cut(s)	486, 655, 1294, 1418, 1752, 2366, 2517, 2808
BmcAI	AGTACT	1 cut(s)	2449
Bme1390I	CCNGG	4 cut(s)	946, 2702, 2742, 3032
Bme18I	GGWCC	3 cut(s)	128, 2386, 2969
BmeT110I	CYCGRG	1 cut(s)	1512
BmgT120I	GGNCC	6 cut(s)	128, 1254, 1255, 1540, 2386, 2969
BmiI	GGNNCC	5 cut(s)	129, 396, 943, 950, 1256
BmrFI	CCNGG	4 cut(s)	946, 2702, 2742, 3032
BmrI	ACTGGG	2 cut(s)	2206, 3386
BmuI	ACTGGG	2 cut(s)	2206, 3386
BpiI	GAAGAC	3 cut(s)	363, 899, 2532
BpmI	CTGGAG	1 cut(s)	1763
BpuEI	CTTGAG	3 cut(s)	635, 2012, 2455
BpuMI	CCSGG	1 cut(s)	946
BsaAI	YACGTR	2 cut(s)	1199, 2981
BsaBI	GATNNNNATC	1 cut(s)	1089
BsaJI	CCNNGG	6 cut(s)	92, 1080, 1688, 2093, 2566, 3031
BsaWI	WCCGGW	3 cut(s)	22, 2473, 3365
Bsc4I	CCNNNNNNNGG	4 cut(s)	1081, 1762, 2061, 3109
Bse118I	RCCGGY	1 cut(s)	22
Bse1I	ACTGG	5 cut(s)	613, 1882, 2201, 2483, 3381
Bse8I	GATNNNNATC	1 cut(s)	1089
BseAI	TCCGGA	1 cut(s)	2473
BseBI	CCWGG	3 cut(s)	2702, 2742, 3032
BseDI	CCNNGG	6 cut(s)	92, 1080, 1688, 2093, 2566, 3031
BseJI	GATNNNNATC	1 cut(s)	1089
BseLI	CCNNNNNNNGG	4 cut(s)	1081, 1762, 2061, 3109
BseMII	CTCAG	3 cut(s)	2499, 2909, 3074
BseNI	ACTGG	5 cut(s)	613, 1882, 2201, 2483, 3381
BseRI	GAGGAG	2 cut(s)	436, 902
BseSI	GKGCMC	1 cut(s)	1258
BseXI	GCAGC	8 cut(s)	496, 665, 1279, 1403, 1737, 2376, 2502, 2793
BseYI	CCCAGC	1 cut(s)	871
BsgI	GTGCAG	1 cut(s)	503
BshFI	GGCC	5 cut(s)	707, 1256, 1541, 1644, 1687
BshTI	ACCGGT	1 cut(s)	22
BsiHKAI	GWGCWC	1 cut(s)	775
BsiHKCI	CYCGRG	1 cut(s)	1512
BsiSI	CCGG	6 cut(s)	23, 945, 1948, 2474, 2903, 3366
BslFI	GGGAC	1 cut(s)	2207
BslI	CCNNNNNNNGG	4 cut(s)	1081, 1762, 2061, 3109
BsmAI	GTCTC	2 cut(s)	848, 1317
BsmFI	GGGAC	1 cut(s)	2207
BsmI	GAATGC	4 cut(s)	350, 691, 1220, 2641
BsnI	GGCC	5 cut(s)	707, 1256, 1541, 1644, 1687
BsoBI	CYCGRG	1 cut(s)	1512
Bsp120I	GGGCCC	1 cut(s)	1254
Bsp1286I	GDGCHC	2 cut(s)	775, 1258
Bsp13I	TCCGGA	1 cut(s)	2473
Bsp1407I	TGTACA	2 cut(s)	2172, 3207
Bsp143I	GATC	8 cut(s)	394, 470, 948, 1818, 1864, 2429, 2905, 2938
Bsp19I	CCATGG	1 cut(s)	1080
BspACI	CCGC	1 cut(s)	1648
BspANI	GGCC	5 cut(s)	707, 1256, 1541, 1644, 1687
BspCNI	CTCAG	3 cut(s)	2500, 2910, 3073
BspEI	TCCGGA	1 cut(s)	2473
BspHI	TCATGA	1 cut(s)	681
BspLI	GGNNCC	5 cut(s)	129, 396, 943, 950, 1256
BspMAI	CTGCAG	1 cut(s)	2756
BspPI	GGATC	9 cut(s)	389, 402, 943, 956, 1813, 1872, 2424, 2913, 2946
BspQI	GCTCTTC	2 cut(s)	901, 3170
BspTI	CTTAAG	1 cut(s)	2390
BsrFI	RCCGGY	1 cut(s)	22
BsrGI	TGTACA	2 cut(s)	2172, 3207
BsrI	ACTGG	5 cut(s)	613, 1882, 2201, 2483, 3381
BssAI	RCCGGY	1 cut(s)	22
BssECI	CCNNGG	6 cut(s)	92, 1080, 1688, 2093, 2566, 3031
BssMI	GATC	8 cut(s)	394, 470, 948, 1818, 1864, 2429, 2905, 2938
BssT1I	CCWWGG	2 cut(s)	92, 1080
Bst2UI	CCWGG	3 cut(s)	2702, 2742, 3032
Bst4CI	ACNGT	5 cut(s)	2049, 2372, 2494, 2567, 3160
Bst6I	CTCTTC	8 cut(s)	54, 310, 413, 901, 971, 2676, 3047, 3170
BstAFI	CTTAAG	1 cut(s)	2390
BstAUI	TGTACA	2 cut(s)	2172, 3207
BstBAI	YACGTR	2 cut(s)	1199, 2981
BstC8I	GCNNGC	5 cut(s)	666, 1559, 1646, 1666, 1748
BstDEI	CTNAG	9 cut(s)	329, 1868, 2000, 2060, 2508, 2540, 2677, 2918, 3060
BstDSI	CCRYGG	4 cut(s)	1080, 1688, 2093, 2566
BstHHI	GCGC	2 cut(s)	691, 1220
BstKTI	GATC	8 cut(s)	397, 473, 951, 1821, 1867, 2432, 2908, 2941
BstMAI	GTCTC	2 cut(s)	848, 1317
BstMBI	GATC	8 cut(s)	394, 470, 948, 1818, 1864, 2429, 2905, 2938
BstMWI	GCNNNNNNNGC	2 cut(s)	653, 1241
BstNI	CCWGG	3 cut(s)	2702, 2742, 3032
BstNSI	RCATGY	2 cut(s)	2179, 2649
BstSCI	CCNGG	4 cut(s)	944, 2700, 2740, 3030
BstSFI	CTRYAG	1 cut(s)	2752
BstSLI	GKGCMC	1 cut(s)	1258
BstV1I	GCAGC	8 cut(s)	496, 665, 1279, 1403, 1737, 2376, 2502, 2793
BstV2I	GAAGAC	3 cut(s)	363, 899, 2532
BstX2I	RGATCY	6 cut(s)	394, 948, 1818, 1864, 2429, 2938
BstXI	CCANNNNNNTGG	1 cut(s)	2100
BstYI	RGATCY	6 cut(s)	394, 948, 1818, 1864, 2429, 2938
BsuI	GTATCC	2 cut(s)	832, 3103
BsuRI	GGCC	5 cut(s)	707, 1256, 1541, 1644, 1687
BtgI	CCRYGG	4 cut(s)	1080, 1688, 2093, 2566
BtsI	GCAGTG	2 cut(s)	494, 2186
BtsIMutI	CAGTG	6 cut(s)	494, 1875, 2186, 2490, 3068, 3374
Cac8I	GCNNGC	5 cut(s)	666, 1559, 1646, 1666, 1748
CciI	TCATGA	1 cut(s)	681
CfoI	GCGC	2 cut(s)	691, 1220
Cfr10I	RCCGGY	1 cut(s)	22
Cfr13I	GGNCC	6 cut(s)	128, 1254, 1255, 1540, 2386, 2969
CseI	GACGC	2 cut(s)	1577, 2954
Csp6I	GTAC	6 cut(s)	407, 2173, 2448, 2801, 2862, 3208
CspAI	ACCGGT	1 cut(s)	22
CspCI	CAANNNNNGTGG	2 cut(s)	697, 732
CviQI	GTAC	6 cut(s)	407, 2173, 2448, 2801, 2862, 3208
DdeI	CTNAG	9 cut(s)	329, 1868, 2000, 2060, 2508, 2540, 2677, 2918, 3060
DpnI	GATC	8 cut(s)	396, 472, 950, 1820, 1866, 2431, 2907, 2940
DpnII	GATC	8 cut(s)	394, 470, 948, 1818, 1864, 2429, 2905, 2938
DraI	TTTAAA	3 cut(s)	504, 622, 880
DraIII	CACNNNGTG	1 cut(s)	315
DrdI	GACNNNNNNGTC	1 cut(s)	2483
DseDI	GACNNNNNNGTC	1 cut(s)	2483
EaeI	YGGCCR	1 cut(s)	1685
Eam1104I	CTCTTC	8 cut(s)	54, 310, 413, 901, 971, 2676, 3047, 3170
EarI	CTCTTC	8 cut(s)	54, 310, 413, 901, 971, 2676, 3047, 3170
Eco130I	CCWWGG	2 cut(s)	92, 1080
Eco24I	GRGCYC	1 cut(s)	1258
Eco32I	GATATC	3 cut(s)	413, 1441, 2145
Eco47I	GGWCC	3 cut(s)	128, 2386, 2969
Eco57I	CTGAAG	3 cut(s)	106, 2157, 2732
Eco72I	CACGTG	1 cut(s)	2981
Eco88I	CYCGRG	1 cut(s)	1512
EcoO109I	RGGNCCY	3 cut(s)	1254, 1255, 1540
EcoRI	GAATTC	2 cut(s)	369, 1002
EcoRII	CCWGG	3 cut(s)	2700, 2740, 3030
EcoRV	GATATC	3 cut(s)	413, 1441, 2145
EcoT14I	CCWWGG	2 cut(s)	92, 1080
EcoT22I	ATGCAT	3 cut(s)	350, 1358, 1599
EcoT38I	GRGCYC	1 cut(s)	1258
ErhI	CCWWGG	2 cut(s)	92, 1080
FalI	AAGNNNNNCTT	2 cut(s)	1147, 1179
FaqI	GGGAC	1 cut(s)	2207
FauI	CCCGC	1 cut(s)	1641
FauNDI	CATATG	1 cut(s)	2960
FblI	GTMKAC	2 cut(s)	723, 1225
Fnu4HI	GCNGC	8 cut(s)	485, 654, 1293, 1417, 1751, 2365, 2516, 2807
FriOI	GRGCYC	1 cut(s)	1258
Fsp4HI	GCNGC	8 cut(s)	485, 654, 1293, 1417, 1751, 2365, 2516, 2807
FspBI	CTAG	8 cut(s)	93, 444, 1209, 1505, 1554, 1572, 2406, 2942
GlaI	GCGC	2 cut(s)	690, 1219
GluI	GCNGC	8 cut(s)	485, 654, 1293, 1417, 1751, 2365, 2516, 2807
GsaI	CCCAGC	1 cut(s)	875
GsuI	CTGGAG	1 cut(s)	1763
HaeIII	GGCC	5 cut(s)	707, 1256, 1541, 1644, 1687
HapII	CCGG	6 cut(s)	23, 945, 1948, 2474, 2903, 3366
HgaI	GACGC	2 cut(s)	1577, 2954
HhaI	GCGC	2 cut(s)	691, 1220
Hin6I	GCGC	2 cut(s)	689, 1218
HinP1I	GCGC	2 cut(s)	689, 1218
HincII	GTYRAC	2 cut(s)	2052, 2488
HindII	GTYRAC	2 cut(s)	2052, 2488
HindIII	AAGCTT	2 cut(s)	1926, 1988
HpaII	CCGG	6 cut(s)	23, 945, 1948, 2474, 2903, 3366
HphI	GGTGA	4 cut(s)	769, 1546, 1669, 2741
Hpy166II	GTNNAC	7 cut(s)	724, 1226, 2052, 2344, 2488, 2500, 3188
Hpy8I	GTNNAC	7 cut(s)	724, 1226, 2052, 2344, 2488, 2500, 3188
Hpy99I	CGWCG	1 cut(s)	1302
HpyCH4III	ACNGT	5 cut(s)	2049, 2372, 2494, 2567, 3160
HpyCH4IV	ACGT	3 cut(s)	1198, 2116, 2980
HpyF10VI	GCNNNNNNNGC	2 cut(s)	653, 1241
HpyF3I	CTNAG	9 cut(s)	329, 1868, 2000, 2060, 2508, 2540, 2677, 2918, 3060
HpySE526I	ACGT	3 cut(s)	1198, 2116, 2980
HspAI	GCGC	2 cut(s)	689, 1218
Kpn2I	TCCGGA	1 cut(s)	2473
Kzo9I	GATC	8 cut(s)	394, 470, 948, 1818, 1864, 2429, 2905, 2938
LguI	GCTCTTC	2 cut(s)	901, 3170
LmnI	GCTCC	4 cut(s)	94, 770, 1134, 2980
Lsp1109I	GCAGC	8 cut(s)	496, 665, 1279, 1403, 1737, 2376, 2502, 2793
MaeI	CTAG	8 cut(s)	93, 444, 1209, 1505, 1554, 1572, 2406, 2942
MaeII	ACGT	3 cut(s)	1198, 2116, 2980
MaeIII	GTNAC	5 cut(s)	2543, 2574, 2733, 2786, 3372
MalI	GATC	8 cut(s)	396, 472, 950, 1820, 1866, 2431, 2907, 2940
MboI	GATC	8 cut(s)	394, 470, 948, 1818, 1864, 2429, 2905, 2938
MfeI	CAATTG	4 cut(s)	1230, 1446, 3019, 3074
MflI	RGATCY	6 cut(s)	394, 948, 1818, 1864, 2429, 2938
MhlI	GDGCHC	2 cut(s)	775, 1258
MlsI	TGGCCA	1 cut(s)	1687
MluNI	TGGCCA	1 cut(s)	1687
MlyI	GAGTC	4 cut(s)	729, 1319, 1866, 2446
MmeI	TCCRAC	4 cut(s)	721, 748, 2049, 3326
Mox20I	TGGCCA	1 cut(s)	1687
Mph1103I	ATGCAT	3 cut(s)	350, 1358, 1599
MroI	TCCGGA	1 cut(s)	2473
MscI	TGGCCA	1 cut(s)	1687
MslI	CAYNNNNRTG	2 cut(s)	2098, 2552
Msp20I	TGGCCA	1 cut(s)	1687
MspA1I	CMGCKG	3 cut(s)	65, 77, 1648
MspCI	CTTAAG	1 cut(s)	2390
MspI	CCGG	6 cut(s)	23, 945, 1948, 2474, 2903, 3366
MspR9I	CCNGG	4 cut(s)	946, 2702, 2742, 3032
MunI	CAATTG	4 cut(s)	1230, 1446, 3019, 3074
Mva1269I	GAATGC	4 cut(s)	350, 691, 1220, 2641
MvaI	CCWGG	3 cut(s)	2702, 2742, 3032
MwoI	GCNNNNNNNGC	2 cut(s)	653, 1241
NciI	CCSGG	1 cut(s)	946
NcoI	CCATGG	1 cut(s)	1080
NdeI	CATATG	1 cut(s)	2960
NdeII	GATC	8 cut(s)	394, 470, 948, 1818, 1864, 2429, 2905, 2938
NlaIV	GGNNCC	5 cut(s)	129, 396, 943, 950, 1256
NmuCI	GTSAC	1 cut(s)	3372
NsiI	ATGCAT	3 cut(s)	350, 1358, 1599
NspI	RCATGY	2 cut(s)	2179, 2649
PagI	TCATGA	1 cut(s)	681
PciI	ACATGT	1 cut(s)	2645
PciSI	GCTCTTC	2 cut(s)	901, 3170
PctI	GAATGC	4 cut(s)	350, 691, 1220, 2641
PflMI	CCANNNNNTGG	1 cut(s)	1762
PinAI	ACCGGT	1 cut(s)	22
PkrI	GCNGC	8 cut(s)	486, 655, 1294, 1418, 1752, 2366, 2517, 2808
PleI	GAGTC	4 cut(s)	728, 1318, 1865, 2445
PmaCI	CACGTG	1 cut(s)	2981
PmlI	CACGTG	1 cut(s)	2981
PpsI	GAGTC	4 cut(s)	728, 1318, 1865, 2445
Ppu21I	YACGTR	2 cut(s)	1199, 2981
PscI	ACATGT	1 cut(s)	2645
PsiI	TTATAA	1 cut(s)	546
Psp6I	CCWGG	3 cut(s)	2700, 2740, 3030
PspCI	CACGTG	1 cut(s)	2981
PspFI	CCCAGC	1 cut(s)	871
PspGI	CCWGG	3 cut(s)	2700, 2740, 3030
PspN4I	GGNNCC	5 cut(s)	129, 396, 943, 950, 1256
PspOMI	GGGCCC	1 cut(s)	1254
PspPI	GGNCC	6 cut(s)	128, 1254, 1255, 1540, 2386, 2969
PstI	CTGCAG	1 cut(s)	2756
PsuI	RGATCY	6 cut(s)	394, 948, 1818, 1864, 2429, 2938
PvuII	CAGCTG	2 cut(s)	65, 77
RsaI	GTAC	6 cut(s)	408, 2174, 2449, 2802, 2863, 3209
RsaNI	GTAC	6 cut(s)	407, 2173, 2448, 2801, 2862, 3208
RseI	CAYNNNNRTG	2 cut(s)	2098, 2552
SapI	GCTCTTC	2 cut(s)	901, 3170
SatI	GCNGC	8 cut(s)	485, 654, 1293, 1417, 1751, 2365, 2516, 2807
Sau3AI	GATC	8 cut(s)	394, 470, 948, 1818, 1864, 2429, 2905, 2938
Sau96I	GGNCC	6 cut(s)	128, 1254, 1255, 1540, 2386, 2969
ScaI	AGTACT	1 cut(s)	2449
SchI	GAGTC	4 cut(s)	729, 1319, 1866, 2446
ScrFI	CCNGG	4 cut(s)	946, 2702, 2742, 3032
SduI	GDGCHC	2 cut(s)	775, 1258
SfcI	CTRYAG	1 cut(s)	2752
SinI	GGWCC	3 cut(s)	128, 2386, 2969
SmiMI	CAYNNNNRTG	2 cut(s)	2098, 2552
SmlI	CTYRAG	4 cut(s)	614, 1991, 2390, 2434
SmoI	CTYRAG	4 cut(s)	614, 1991, 2390, 2434
SsiI	CCGC	1 cut(s)	1648
SspI	AATATT	1 cut(s)	2236
SspMI	CTAG	8 cut(s)	93, 444, 1209, 1505, 1554, 1572, 2406, 2942
StyD4I	CCNGG	4 cut(s)	944, 2700, 2740, 3030
StyI	CCWWGG	2 cut(s)	92, 1080
TaaI	ACNGT	5 cut(s)	2049, 2372, 2494, 2567, 3160
TaiI	ACGT	3 cut(s)	1201, 2119, 2983
TaqI	TCGA	9 cut(s)	169, 601, 1261, 1297, 2328, 2349, 2401, 3123, 3264
TatI	WGTACW	4 cut(s)	2172, 2447, 2800, 3207
TscAI	CASTG	6 cut(s)	494, 1882, 2186, 2497, 3068, 3381
TseFI	GTSAC	1 cut(s)	3372
TseI	GCWGC	8 cut(s)	484, 653, 1292, 1416, 1750, 2364, 2515, 2806
Tsp45I	GTSAC	1 cut(s)	3372
TspGWI	ACGGA	5 cut(s)	741, 1293, 1596, 1705, 2110
TspRI	CASTG	6 cut(s)	494, 1882, 2186, 2497, 3068, 3381
Van91I	CCANNNNNTGG	1 cut(s)	1762
Vha464I	CTTAAG	1 cut(s)	2390
VpaK11BI	GGWCC	3 cut(s)	128, 2386, 2969
XapI	RAATTY	6 cut(s)	369, 517, 596, 846, 1002, 3169
XbaI	TCTAGA	1 cut(s)	2941
XceI	RCATGY	2 cut(s)	2179, 2649
XcmI	CCANNNNNNNNNTGG	1 cut(s)	316
XmaJI	CCTAGG	1 cut(s)	92
XmiI	GTMKAC	2 cut(s)	723, 1225
XspI	CTAG	8 cut(s)	93, 444, 1209, 1505, 1554, 1572, 2406, 2942
ZrmI	AGTACT	1 cut(s)	2449
Zsp2I	ATGCAT	3 cut(s)	350, 1358, 1599

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.