Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr6B

Orthogroup ID

OG0007152

Physical Location & Seq

Genomic Coordinates Reverse (-)

54568225 .. 54575580

Gene Structure 7356 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh6BG315900.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2607 bp

ATGGCGGACCCTCAGAACCAGAAGCCATGGAAGGTCGAGTACGCCAAGTCTTCACGATCGTCATGCAAAACCTGCAGAAACCCCATTGAAAAAGAGAACCTCCGCTTCGGCAAAATGGTCCAGGCCAAACAATTCGACGGCTTCATGCCCATGTGGAACCATGCTAGTTGCATAATGAAGAAGGCGAAGCAGATAAAATCAACTGATGATGTTGAAGGCCTTGAGCTCCTCCGGTGGGAAGATCAGAAGAAAATTAGAGATTATGTACAAAGTGGTGGATCTGGTGGACCCTCCGATACAACAACTACAAGCAAGGCCAGTGCTACAGAAAGTGGTATTGAAGTTTCGCAAACTTCTCGGGCTACTTGCAGGCTCTGCAGCCAAAAGATTTTAAAAGGGGAGGTCCGTATATCAACCAAGCCTGAAGGTCAAGGAGCTAGGGGCTTGGCATGGCACCATGCCAAGTGTTTCATGGAATCATCACCATCTACCCAAGTGGAGAAGCTGTCTGGATGGGAGACCATCCCAGTTTCTGATCAGGCAGCTGTGTCTGCCTTAGTTAAGGATGGCAAAGAAGTTAAAGAGATCCTGCCACAGTCTACTTCTAAAGCTGGCACTAAACGAAGAAAAGATGGGGATGGTGATCAGAAGTCAAAAGTTTCTAAGTCGGAAGGTGACGTGTCAACAAGCAGGGATGTGTCTGTGAGTAATGCTACTGATATGGAGAGCAAAATGGAGGCGCAAACTAAAGAGCTGTGGGCTCTGAAAGATGATCTCAAAAAGCATGTGACAACTGTAGAGCTGCGTAAAATGCTTGAAGCCAATGCTCAAGATTCAACAGGATCAGAGCTTGATTTGCGTGAGCGCTGCGCTGATGGAATGATGTTTGGAGCACTTAGTACCTGCCCACTCTGTTCCGATCATCTTCACTATTCTGGAGCCATGTACAGGTGCCATGGGTTCCTGACTGCATGGAGCAAGTGTTCTTACTCTACTCAAGAAACTGAACGTTGTAAAGGGAAGTGGAAAATCCCAGAAGATACAGATAATCAATTTCTGCAGAAGTGGTTTAAATCACAGAAGGTTAGCAAACCTACACGAATACTGCCACCGCCATCATCTAACAATCCTGGAGGTCAAGCTTTCAATGGCCAATCTCAGTCTGGTGCAAGCTTGGGGGATTTGAAAGTTTCTTTTCGTGGATTACCAAAAGAATCCATGGAAAAGTGGAATAAAGATATAGAGGGTGTAGCTGGGTCCGTTCATGCTAAGATAAAAAAAGATACTAATTGCTTGGTTGTGGGTGGAGGACTGGATGATAAAGATGCTGAGATGAAAAAAGCAAGGAGGATGAAAATACCAATTGTTAGGGAGGATTATCTGGTTGATTGCTTTAAAAGAAAGAAGAAGCTTCCATTTGATCTTTACAAAGTTGAAGCTGTTGGTGAAGCCTCTAGCATGGTCACTGTCAAAGTGAAAGGGCGAAGTGCCGTTCATGAATCTTCTGGATTGCAAGATACAGGTCACATACTTGAGGATGGAAAAAGCATATATAATACAACTTTAAGCATGTCTGATTTGTCAACCGGTGTTAATAGTTATTACATCCTCCAAATTATACAAGATGATAAAAGTTCAGACTGCCATGTGTTCCGTAAATGGGGACGAGTAGGCAATGACAAAATTGGAGGAACCAAACTAGAAGAGATGTCAAAATATGATGCCATATCTGATTTTAAACGTTTGTTTCTTGAGAAGACTGGCAATTCATGGGAAGCATGGGAGAAAAAGCAAGATTTTCGGAAGCAACCTGGAAAATTCTTTCCATTGGATATTGATTATGGTGTTAACAAAGAGGTTTCAAAGAAAAATCAGAACAATGAACCCAGCAAATTGCCTCCTCAGTTAGCAGAGTTGATGAAGATGCTCTTCAACGTTGAAACATACAGAGCTGCCATGATGGAATTTGAGATAAATATGTCAGAGATGCCACTTGGAAAACTGAGTAAAAGCAATATCCAAAAGGGGTTTGAGGCATTAACGGAAATACAGAATCTATTAAAGAGTGATGGTGCCTCTTCCATAAAAGATAGCTTGATTGTAGATGCAAGCAATCGATTTTTCACAGTGATACCTTCCATACATCCGCATATTATTAGGGATGAGGATGACTTTAAGTCAAAGTTGAAAATGCTAGAAGCTCTCCAGGACATTGAAATTGCTTCCAGATTAGTCGGTTTTGATGCTGATAGTGATGACTCTTTGGATGAGAAGTATAAGAAGCTCCGGTGTTGTATGAATCCACTTCCTCACGATAGTGAAGATTATCGGTTGATTGAAAAGTATCTTCTTACTACTCATGCCCCTACACATACGTATATGGATAAACCTCCTAAGGGAAAGCACTCTACGAAGGGACTTGGCAAGAAAAAACCTGAGGAGTCGGACTATGTTAAATGGAGAGATGATGTCACTGTGCCCTGTGGCAAACCAGTGGATTCACATGTCAGGGCCTCTGAGCTGATGTATAATGAGTATATTGTTTATGATACAGCTCAAGTTAAGATGCAATTTCTATTGAAGGTGAAGTTTCATCACAAGAGATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0003824 GO:0003909 GO:0003910 GO:0003950 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006266 GO:0006271 GO:0006273 GO:0006281 GO:0006464 GO:0006471 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006979 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009719 GO:0009725 GO:0009737 GO:0009987 GO:0010033 GO:0016070 GO:0016740 GO:0016757 GO:0016763 GO:0016874 GO:0016886 GO:0019538 GO:0022616 GO:0033554 GO:0033993 GO:0034641 GO:0034645 GO:0036211 GO:0042221 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0046483 GO:0050896 GO:0051103 GO:0051716 GO:0071704 GO:0090304 GO:0097305 GO:0140097 GO:1901360 GO:1901564 GO:1901576 GO:1901700

KEGG Pathways

Metabolic & Signaling

ko03410 ko04210 ko04212 ko04214 ko04217 map03410 map04210 map04212 map04214 map04217

Pfam Domains

Protein Families

BRCT PADR1 PARP PARP_reg WGR zf-PARP

Protein Analysis

868

Amino Acids

97.69

Weight (kDa)

8.83

Isoelectric Point (pI)

43.52

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
zf-PARP	PF00645	13 - 88	5.8e-22	Poly(ADP-ribose) polymerase and DNA-Ligase Zn-finger region
zf-PARP	PF00645	114 - 185	4.1e-12	Poly(ADP-ribose) polymerase and DNA-Ligase Zn-finger region
PADR1_N	PF21728	239 - 297	8.9e-22	PADR1, N-terminal helical domain
Zn_ribbon_PADR1	PF08063	298 - 338	9e-12	PADR1 domain, zinc ribbon fold
BRCT	PF00533	394 - 465	1.9e-08	BRCA1 C Terminus (BRCT) domain
WGR	PF05406	518 - 597	6.8e-17	WGR domain
PARP_reg	PF02877	630 - 759	4e-37	Poly(ADP-ribose) polymerase, regulatory domain
PARP	PF00644	792 - 864	6.3e-20	Poly(ADP-ribose) polymerase catalytic domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0007152)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G31320
fragaria_vesca	FvH4_2g21980 FvH4_2g21980 FvH4_2g21980
malus_domestica	MD05G1201000.v1.1 MD10G1187800.v1.1
prunus_persica	Prupe.8G227600_v2.0.a1 Prupe.8G227600_v2.0.a1
pyrus_communis	pycom05g18780 pycom10g16230
rosa_chinensis	RchiOBHm_Chr6g0288861
rosa_laevigata	RLG00000012360
rosa_multiflora	Rmu_sc0005314.1_g000009
rosa_roxburghii	Rroxscaffold_7G00179830
rosa_rugosa	Rorug06G0198600 Rorug06G0198600 Rorug06G0198600 Rorug06G0198700 Rorug06G0198800 Rorug06G0198900 Rorug06G0198900
rosa_samantha	Rh6AG310000 Rh6BG315900 Rh6CG323400
rosa_wichuraiana	Rw6G026810

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	2 cut(s)	80, 911
AccB1I	GGYRCC	3 cut(s)	453, 951, 2072
AccI	GTMKAC	1 cut(s)	599
AciI	CCGC	4 cut(s)	5, 103, 1112, 2147
AclI	AACGTT	3 cut(s)	1009, 1741, 1935
AclWI	GGATC	3 cut(s)	286, 580, 850
AcoI	YGGCCR	1 cut(s)	1150
AcsI	RAATTY	2 cut(s)	1817, 1964
AcuI	CTGAAG	1 cut(s)	444
AfaI	GTAC	4 cut(s)	41, 267, 901, 947
AfeI	AGCGCT	1 cut(s)	866
AfiI	CCNNNNNNNGG	4 cut(s)	235, 948, 1660, 2396
AflIII	ACRYGT	2 cut(s)	678, 2503
AgeI	ACCGGT	1 cut(s)	1586
AhdI	GACNNNNNGTC	1 cut(s)	2176
AjiI	CACGTC	1 cut(s)	679
AjnI	CCWGG	4 cut(s)	120, 1129, 1810, 2205
AleI	CACNNNNGTG	1 cut(s)	2316
AloI	GAACNNNNNNTCC	2 cut(s)	967, 999
Alw21I	GWGCWC	2 cut(s)	228, 895
Alw26I	GTCTC	1 cut(s)	512
AlwI	GGATC	3 cut(s)	286, 580, 850
AlwNI	CAGNNNCTG	2 cut(s)	375, 533
Ama87I	CYCGRG	1 cut(s)	357
Aor51HI	AGCGCT	1 cut(s)	866
AoxI	GGCC	5 cut(s)	123, 217, 315, 1150, 2511
ApeKI	GCWGC	5 cut(s)	378, 542, 802, 867, 1952
ApoI	RAATTY	2 cut(s)	1817, 1964
AsiGI	ACCGGT	1 cut(s)	1586
AspLEI	GCGC	3 cut(s)	742, 867, 872
AspS9I	GGNCC	6 cut(s)	7, 118, 287, 403, 1257, 2511
AsuHPI	GGTGA	5 cut(s)	474, 653, 686, 1457, 2596
AvaI	CYCGRG	1 cut(s)	357
AvaII	GGWCC	5 cut(s)	7, 118, 287, 403, 1257
AxyI	CCTNAGG	2 cut(s)	2394, 2436
BaeGI	GKGCMC	1 cut(s)	2481
BalI	TGGCCA	1 cut(s)	1152
BanI	GGYRCC	3 cut(s)	453, 951, 2072
BanII	GRGCYC	2 cut(s)	228, 763
BarI	GAAGNNNNNNTAC	2 cut(s)	23, 55
BbsI	GAAGAC	2 cut(s)	42, 1763
Bbv12I	GWGCWC	2 cut(s)	228, 895
BbvI	GCAGC	5 cut(s)	390, 554, 789, 854, 1939
BceAI	ACGGC	2 cut(s)	154, 1475
BcgI	CGANNNNNNTGC	6 cut(s)	45, 79, 338, 372, 1781, 1815
BciT130I	CCWGG	4 cut(s)	122, 1131, 1812, 2207
BclI	TGATCA	2 cut(s)	535, 643
BcoDI	GTCTC	1 cut(s)	512
BfaI	CTAG	5 cut(s)	165, 438, 1455, 1700, 2195
BfmI	CTRYAG	5 cut(s)	73, 324, 376, 795, 1058
BfoI	RGCGCY	1 cut(s)	868
BfuAI	ACCTGC	2 cut(s)	80, 911
BisI	GCNGC	5 cut(s)	379, 543, 803, 868, 1953
BlsI	GCNGC	5 cut(s)	380, 544, 804, 869, 1954
Bme1390I	CCNGG	4 cut(s)	122, 1131, 1812, 2207
Bme18I	GGWCC	5 cut(s)	7, 118, 287, 403, 1257
BmeRI	GACNNNNNGTC	1 cut(s)	2176
BmeT110I	CYCGRG	1 cut(s)	357
BmgBI	CACGTC	1 cut(s)	679
BmgT120I	GGNCC	6 cut(s)	7, 118, 287, 403, 1257, 2511
BmrFI	CCNGG	4 cut(s)	122, 1131, 1812, 2207
BmrI	ACTGGG	1 cut(s)	521
BmsI	GCATC	7 cut(s)	1315, 1711, 1914, 1977, 2095, 2233, 2556
BmuI	ACTGGG	1 cut(s)	521
BpiI	GAAGAC	2 cut(s)	42, 1763
BpmI	CTGGAG	3 cut(s)	957, 1152, 2189
BpuEI	CTTGAG	6 cut(s)	242, 813, 981, 1553, 1772, 2541
Bsa29I	ATCGAT	1 cut(s)	2116
BsaAI	YACGTR	1 cut(s)	2376
BsaBI	GATNNNNATC	1 cut(s)	642
BsaI	GGTCTC	1 cut(s)	512
BsaJI	CCNNGG	3 cut(s)	26, 955, 1218
BsaWI	WCCGGW	3 cut(s)	231, 1586, 2286
BsaXI	ACNNNNNCTCC	4 cut(s)	491, 521, 967, 997
Bsc4I	CCNNNNNNNGG	4 cut(s)	235, 948, 1660, 2396
Bse118I	RCCGGY	1 cut(s)	1586
Bse1I	ACTGG	5 cut(s)	318, 527, 1317, 1765, 2492
Bse21I	CCTNAGG	2 cut(s)	2394, 2436
Bse3DI	GCAATG	1 cut(s)	1681
Bse8I	GATNNNNATC	1 cut(s)	642
BseBI	CCWGG	4 cut(s)	122, 1131, 1812, 2207
BseCI	ATCGAT	1 cut(s)	2116
BseDI	CCNNGG	3 cut(s)	26, 955, 1218
BseJI	GATNNNNATC	1 cut(s)	642
BseLI	CCNNNNNNNGG	4 cut(s)	235, 948, 1660, 2396
BseMI	GCAATG	1 cut(s)	1681
BseMII	CTCAG	7 cut(s)	26, 1172, 1320, 1916, 1994, 2427, 2508
BseNI	ACTGG	5 cut(s)	318, 527, 1317, 1765, 2492
BseRI	GAGGAG	3 cut(s)	218, 1890, 2453
BseSI	GKGCMC	1 cut(s)	2481
BseXI	GCAGC	5 cut(s)	390, 554, 789, 854, 1939
BseYI	CCCAGC	2 cut(s)	1253, 1886
Bsh1285I	CGRYCG	1 cut(s)	59
BshFI	GGCC	5 cut(s)	125, 219, 317, 1152, 2513
BshNI	GGYRCC	3 cut(s)	453, 951, 2072
BshTI	ACCGGT	1 cut(s)	1586
BshVI	ATCGAT	1 cut(s)	2116
BsiEI	CGRYCG	1 cut(s)	59
BsiHKAI	GWGCWC	2 cut(s)	228, 895
BsiHKCI	CYCGRG	1 cut(s)	357
BsiSI	CCGG	3 cut(s)	232, 1587, 2287
BslFI	GGGAC	2 cut(s)	1677, 2430
BslI	CCNNNNNNNGG	4 cut(s)	235, 948, 1660, 2396
BsmAI	GTCTC	1 cut(s)	512
BsmFI	GGGAC	2 cut(s)	1677, 2430
BsnI	GGCC	5 cut(s)	125, 219, 317, 1152, 2513
Bso31I	GGTCTC	1 cut(s)	512
BsoBI	CYCGRG	1 cut(s)	357
Bsp1286I	GDGCHC	4 cut(s)	228, 763, 895, 2481
Bsp1407I	TGTACA	2 cut(s)	265, 945
Bsp19I	CCATGG	3 cut(s)	26, 955, 1218
BspACI	CCGC	4 cut(s)	5, 103, 1112, 2147
BspANI	GGCC	5 cut(s)	125, 219, 317, 1152, 2513
BspCNI	CTCAG	7 cut(s)	25, 1171, 1321, 1915, 1995, 2428, 2509
BspDI	ATCGAT	1 cut(s)	2116
BspHI	TCATGA	1 cut(s)	1495
BspMAI	CTGCAG	3 cut(s)	77, 380, 1062
BspMI	ACCTGC	2 cut(s)	80, 911
BspPI	GGATC	3 cut(s)	286, 580, 850
BspQI	GCTCTTC	1 cut(s)	1934
BspT107I	GGYRCC	3 cut(s)	453, 951, 2072
BspTNI	GGTCTC	1 cut(s)	512
BsrDI	GCAATG	1 cut(s)	1681
BsrFI	RCCGGY	1 cut(s)	1586
BsrGI	TGTACA	2 cut(s)	265, 945
BsrI	ACTGG	5 cut(s)	318, 527, 1317, 1765, 2492
BssAI	RCCGGY	1 cut(s)	1586
BssECI	CCNNGG	3 cut(s)	26, 955, 1218
BssT1I	CCWWGG	3 cut(s)	26, 955, 1218
Bst2UI	CCWGG	4 cut(s)	122, 1131, 1812, 2207
Bst4CI	ACNGT	5 cut(s)	597, 796, 1468, 2128, 2476
Bst6I	CTCTTC	3 cut(s)	1698, 1934, 2083
BstAPI	GCANNNNNTGC	2 cut(s)	72, 375
BstAUI	TGTACA	2 cut(s)	265, 945
BstBAI	YACGTR	1 cut(s)	2376
BstC8I	GCNNGC	4 cut(s)	371, 613, 1171, 2110
BstDSI	CCRYGG	3 cut(s)	26, 955, 1218
BstH2I	RGCGCY	1 cut(s)	868
BstHHI	GCGC	3 cut(s)	742, 867, 872
BstMAI	GTCTC	1 cut(s)	512
BstMCI	CGRYCG	1 cut(s)	59
BstMWI	GCNNNNNNNGC	5 cut(s)	72, 375, 551, 811, 856
BstNI	CCWGG	4 cut(s)	122, 1131, 1812, 2207
BstNSI	RCATGY	3 cut(s)	788, 1573, 2507
BstSCI	CCNGG	4 cut(s)	120, 1129, 1810, 2205
BstSFI	CTRYAG	5 cut(s)	73, 324, 376, 795, 1058
BstSLI	GKGCMC	1 cut(s)	2481
BstSNI	TACGTA	1 cut(s)	2376
BstV1I	GCAGC	5 cut(s)	390, 554, 789, 854, 1939
BstV2I	GAAGAC	2 cut(s)	42, 1763
BstX2I	RGATCY	2 cut(s)	278, 585
BstYI	RGATCY	2 cut(s)	278, 585
Bsu15I	ATCGAT	1 cut(s)	2116
Bsu36I	CCTNAGG	2 cut(s)	2394, 2436
BsuRI	GGCC	5 cut(s)	125, 219, 317, 1152, 2513
BsuTUI	ATCGAT	1 cut(s)	2116
BtgI	CCRYGG	3 cut(s)	26, 955, 1218
BtrI	CACGTC	1 cut(s)	679
BtsIMutI	CAGTG	5 cut(s)	325, 1464, 2133, 2472, 2499
BveI	ACCTGC	2 cut(s)	80, 911
Cac8I	GCNNGC	4 cut(s)	371, 613, 1171, 2110
CaiI	CAGNNNCTG	2 cut(s)	375, 533
CciI	TCATGA	1 cut(s)	1495
CfoI	GCGC	3 cut(s)	742, 867, 872
Cfr10I	RCCGGY	1 cut(s)	1586
Cfr13I	GGNCC	6 cut(s)	7, 118, 287, 403, 1257, 2511
ClaI	ATCGAT	1 cut(s)	2116
Csp6I	GTAC	4 cut(s)	40, 266, 900, 946
CspAI	ACCGGT	1 cut(s)	1586
CviQI	GTAC	4 cut(s)	40, 266, 900, 946
DraI	TTTAAA	4 cut(s)	393, 1072, 1396, 1738
DriI	GACNNNNNGTC	1 cut(s)	2176
EaeI	YGGCCR	1 cut(s)	1150
Eam1104I	CTCTTC	3 cut(s)	1698, 1934, 2083
Eam1105I	GACNNNNNGTC	1 cut(s)	2176
EarI	CTCTTC	3 cut(s)	1698, 1934, 2083
EciI	GGCGGA	1 cut(s)	20
Ecl136II	GAGCTC	1 cut(s)	226
Eco105I	TACGTA	1 cut(s)	2376
Eco130I	CCWWGG	3 cut(s)	26, 955, 1218
Eco147I	AGGCCT	1 cut(s)	219
Eco24I	GRGCYC	2 cut(s)	228, 763
Eco31I	GGTCTC	1 cut(s)	512
Eco47I	GGWCC	5 cut(s)	7, 118, 287, 403, 1257
Eco47III	AGCGCT	1 cut(s)	866
Eco53kI	GAGCTC	1 cut(s)	226
Eco57I	CTGAAG	1 cut(s)	444
Eco81I	CCTNAGG	2 cut(s)	2394, 2436
Eco88I	CYCGRG	1 cut(s)	357
EcoICRI	GAGCTC	1 cut(s)	226
EcoO109I	RGGNCCY	1 cut(s)	2511
EcoRII	CCWGG	4 cut(s)	120, 1129, 1810, 2205
EcoT14I	CCWWGG	3 cut(s)	26, 955, 1218
EcoT38I	GRGCYC	2 cut(s)	228, 763
ErhI	CCWWGG	3 cut(s)	26, 955, 1218
FalI	AAGNNNNNCTT	2 cut(s)	1913, 1945
FaqI	GGGAC	2 cut(s)	1677, 2430
FbaI	TGATCA	2 cut(s)	535, 643
FblI	GTMKAC	1 cut(s)	599
Fnu4HI	GCNGC	5 cut(s)	379, 543, 803, 868, 1953
FriOI	GRGCYC	2 cut(s)	228, 763
Fsp4HI	GCNGC	5 cut(s)	379, 543, 803, 868, 1953
FspBI	CTAG	5 cut(s)	165, 438, 1455, 1700, 2195
GlaI	GCGC	3 cut(s)	741, 866, 871
GluI	GCNGC	5 cut(s)	379, 543, 803, 868, 1953
GsaI	CCCAGC	2 cut(s)	1257, 1890
GsuI	CTGGAG	3 cut(s)	957, 1152, 2189
HaeII	RGCGCY	1 cut(s)	868
HaeIII	GGCC	5 cut(s)	125, 219, 317, 1152, 2513
HapII	CCGG	3 cut(s)	232, 1587, 2287
HhaI	GCGC	3 cut(s)	742, 867, 872
Hin6I	GCGC	3 cut(s)	740, 865, 870
HinP1I	GCGC	3 cut(s)	740, 865, 870
HincII	GTYRAC	3 cut(s)	684, 1584, 1849
HindII	GTYRAC	3 cut(s)	684, 1584, 1849
HindIII	AAGCTT	3 cut(s)	1140, 1171, 1409
HinfI	GANTC	9 cut(s)	476, 833, 1214, 1499, 2053, 2258, 2299, 2441, 2498
HpaI	GTTAAC	1 cut(s)	1849
HpaII	CCGG	3 cut(s)	232, 1587, 2287
HphI	GGTGA	5 cut(s)	474, 653, 686, 1457, 2596
Hpy166II	GTNNAC	5 cut(s)	287, 600, 684, 1584, 1849
Hpy8I	GTNNAC	5 cut(s)	287, 600, 684, 1584, 1849
Hpy99I	CGWCG	1 cut(s)	140
HpyAV	CCTTC	9 cut(s)	25, 175, 209, 419, 665, 1075, 2145, 2407, 2575
HpyCH4III	ACNGT	5 cut(s)	597, 796, 1468, 2128, 2476
HpyCH4IV	ACGT	5 cut(s)	678, 1009, 1741, 1935, 2375
HpyF10VI	GCNNNNNNNGC	5 cut(s)	72, 375, 551, 811, 856
HpySE526I	ACGT	5 cut(s)	678, 1009, 1741, 1935, 2375
HspAI	GCGC	3 cut(s)	740, 865, 870
Ksp22I	TGATCA	2 cut(s)	535, 643
KspAI	GTTAAC	1 cut(s)	1849
LguI	GCTCTTC	1 cut(s)	1934
LmnI	GCTCC	6 cut(s)	231, 434, 890, 938, 975, 2289
Lsp1109I	GCAGC	5 cut(s)	390, 554, 789, 854, 1939
LweI	GCATC	7 cut(s)	1315, 1711, 1914, 1977, 2095, 2233, 2556
MaeI	CTAG	5 cut(s)	165, 438, 1455, 1700, 2195
MaeII	ACGT	5 cut(s)	678, 1009, 1741, 1935, 2375
MaeIII	GTNAC	5 cut(s)	674, 787, 1462, 1523, 2470
MfeI	CAATTG	1 cut(s)	1362
MflI	RGATCY	2 cut(s)	278, 585
MhlI	GDGCHC	4 cut(s)	228, 763, 895, 2481
MlsI	TGGCCA	1 cut(s)	1152
MluNI	TGGCCA	1 cut(s)	1152
MlyI	GAGTC	2 cut(s)	2252, 2450
MmeI	TCCRAC	2 cut(s)	648, 2424
Mox20I	TGGCCA	1 cut(s)	1152
MscI	TGGCCA	1 cut(s)	1152
MslI	CAYNNNNRTG	2 cut(s)	149, 2316
Msp20I	TGGCCA	1 cut(s)	1152
MspA1I	CMGCKG	1 cut(s)	545
MspI	CCGG	3 cut(s)	232, 1587, 2287
MspR9I	CCNGG	4 cut(s)	122, 1131, 1812, 2207
MteI	GCGCNGCGC	1 cut(s)	868
MunI	CAATTG	1 cut(s)	1362
MvaI	CCWGG	4 cut(s)	122, 1131, 1812, 2207
MwoI	GCNNNNNNNGC	5 cut(s)	72, 375, 551, 811, 856
NcoI	CCATGG	3 cut(s)	26, 955, 1218
NmuCI	GTSAC	5 cut(s)	674, 787, 1462, 1523, 2470
NspI	RCATGY	3 cut(s)	788, 1573, 2507
OliI	CACNNNNGTG	1 cut(s)	2316
PagI	TCATGA	1 cut(s)	1495
PceI	AGGCCT	1 cut(s)	219
PciI	ACATGT	1 cut(s)	2503
PciSI	GCTCTTC	1 cut(s)	1934
PfeI	GAWTC	7 cut(s)	476, 833, 1214, 1499, 2053, 2299, 2498
PfoI	TCCNGGA	2 cut(s)	1129, 2205
PinAI	ACCGGT	1 cut(s)	1586
PkrI	GCNGC	5 cut(s)	380, 544, 804, 869, 1954
Ple19I	CGATCG	1 cut(s)	59
PleI	GAGTC	2 cut(s)	2252, 2449
PpsI	GAGTC	2 cut(s)	2252, 2449
Ppu21I	YACGTR	1 cut(s)	2376
PscI	ACATGT	1 cut(s)	2503
Psp124BI	GAGCTC	1 cut(s)	228
Psp1406I	AACGTT	3 cut(s)	1009, 1741, 1935
Psp6I	CCWGG	4 cut(s)	120, 1129, 1810, 2205
PspFI	CCCAGC	2 cut(s)	1253, 1886
PspGI	CCWGG	4 cut(s)	120, 1129, 1810, 2205
PspPI	GGNCC	6 cut(s)	7, 118, 287, 403, 1257, 2511
PstI	CTGCAG	3 cut(s)	77, 380, 1062
PstNI	CAGNNNCTG	2 cut(s)	375, 533
PsuI	RGATCY	2 cut(s)	278, 585
PvuI	CGATCG	1 cut(s)	59
PvuII	CAGCTG	1 cut(s)	545
RsaI	GTAC	4 cut(s)	41, 267, 901, 947
RsaNI	GTAC	4 cut(s)	40, 266, 900, 946
RseI	CAYNNNNRTG	2 cut(s)	149, 2316
SacI	GAGCTC	1 cut(s)	228
SapI	GCTCTTC	1 cut(s)	1934
SatI	GCNGC	5 cut(s)	379, 543, 803, 868, 1953
Sau96I	GGNCC	6 cut(s)	7, 118, 287, 403, 1257, 2511
SchI	GAGTC	2 cut(s)	2252, 2450
ScrFI	CCNGG	4 cut(s)	122, 1131, 1812, 2207
SduI	GDGCHC	4 cut(s)	228, 763, 895, 2481
SfaNI	GCATC	7 cut(s)	1315, 1711, 1914, 1977, 2095, 2233, 2556
SfcI	CTRYAG	5 cut(s)	73, 324, 376, 795, 1058
SinI	GGWCC	5 cut(s)	7, 118, 287, 403, 1257
SmiMI	CAYNNNNRTG	2 cut(s)	149, 2316
SmlI	CTYRAG	6 cut(s)	221, 828, 996, 1532, 1751, 2556
SmoI	CTYRAG	6 cut(s)	221, 828, 996, 1532, 1751, 2556
SnaBI	TACGTA	1 cut(s)	2376
SseBI	AGGCCT	1 cut(s)	219
SsiI	CCGC	4 cut(s)	5, 103, 1112, 2147
SspMI	CTAG	5 cut(s)	165, 438, 1455, 1700, 2195
SstI	GAGCTC	1 cut(s)	228
StuI	AGGCCT	1 cut(s)	219
StyD4I	CCNGG	4 cut(s)	120, 1129, 1810, 2205
StyI	CCWWGG	3 cut(s)	26, 955, 1218
TaaI	ACNGT	5 cut(s)	597, 796, 1468, 2128, 2476
TaiI	ACGT	5 cut(s)	681, 1012, 1744, 1938, 2378
TaqI	TCGA	3 cut(s)	36, 135, 2116
TatI	WGTACW	2 cut(s)	265, 945
TfiI	GAWTC	7 cut(s)	476, 833, 1214, 1499, 2053, 2299, 2498
TscAI	CASTG	5 cut(s)	325, 1471, 2133, 2479, 2499
TseFI	GTSAC	5 cut(s)	674, 787, 1462, 1523, 2470
TseI	GCWGC	5 cut(s)	378, 542, 802, 867, 1952
Tsp45I	GTSAC	5 cut(s)	674, 787, 1462, 1523, 2470
TspGWI	ACGGA	4 cut(s)	395, 1249, 1643, 2057
TspRI	CASTG	5 cut(s)	325, 1471, 2133, 2479, 2499
VpaK11BI	GGWCC	5 cut(s)	7, 118, 287, 403, 1257
XapI	RAATTY	2 cut(s)	1817, 1964
XceI	RCATGY	3 cut(s)	788, 1573, 2507
XcmI	CCANNNNNNNNNTGG	2 cut(s)	469, 1160
XmiI	GTMKAC	1 cut(s)	599
XspI	CTAG	5 cut(s)	165, 438, 1455, 1700, 2195

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.