Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb2

Orthogroup ID

OG0000811

Physical Location & Seq

Genomic Coordinates Reverse (-)

18139112 .. 18146949

Gene Structure 7838 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_2g21980.t3

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2874 bp

ATGGCGGACCCTCAGAATCAGAAGCCATGGAAGGTCGAGTACGCCAAGTCGTCACGATCGTCATGCAAAACTTGCAGAAGCCCCATTGAGAAAGAGAACCTCCGCTTCGGCAAGATGGTTCAGGCCACCCAATTCGACGGCTTCATACCTATGTGGAACCATGCCAGTTGCATAATGAAGAAGGCGAAGCAGATAAAATCAACTGATGATATTGAAGGCCTTGAGCTGCTCCGGTGGGAGGATCAGAAGAAAATTAGAGATTATGTACAAAGTGGTGCATCAGCGGGTCCTGCTGGACCATCATCGGATACAAAAACTACAAGCAAGGCCAGTGGTATTGAAGTTTCACAAACTTCTCGGGCTACTTGCAGGCTCTGCAGCCAAAGGATTTTAAAAGGGGAGGTTCGTATATCAACAAAGCTTGAAGGTCAAGGAGCTAGGGGGTTAGCATGGCACCATGCCAAGTGTTTCATGGAATCATCACCATCTACCCAGGTGGAGAAGCTGTCTGGATGGGAGACTATCTCAGTTTCTGATCAGGCAGCTGTTTCTGCCTTGCTTAAGGATGGCAAAAAAGTTGAGGCACAAGAGAGTAAAGAGATCCCGCCACAGTCAACTTCTAAGGCTGGCACTAAACGAAGGAAAGAGGGGGATGGTGACGATCAGAAGTCAAAAGTTTCTAAGTCGGAAGGTGATGTGTCAACAAGCAGGGATGTGTCTGTTAGTAATGCTACTGAAGTGGAGATCAAAATGGAGGTGCAAACTAAAGAGCTGTGGGCTCTGAAGGATGATCTCAAAAAGCATGTGACAACTGTAGAGATGCGTAAAATGCTTGAAGCCAATGCTCAGAGTTCAACAGGATCAGAGCTTGATTTGCGTGACCTCTGGTGTCATGGGTTCCTGACTGCATGGACCAAGTGTTCTTACTCCACTCAAGAACCTGAACGTCTTAAAGGGAAGTGGAAAGTCCCAGAAGATACAGAGAATCAATTTCTGCAAAAGTGGTTTAAATCACAGAAGGTTGGCAAACCTGCACGAATACTGCCACCACCATCATCTAACTGTCCTGGAGGTCAGGCTTTGAACGGCCAACCTCAGTCTAGTGCAAGCTTGGCTGATTTGAAAGTTTCTTTTCGTGGATTACCAAAAGAATCCATGGAAAAGTGGAATAAAGATATAGAGGGTGTAACTGGATCCGTTCATGCCAAGATAAAAAAAGATACTAATTGCTTAGTTGTGGGTGGAGAACCGGATGCTAAAGATGCTGAGATCAAAAAAGCAAGGAGGATGAAAATACCAATTGTTAGGGAGGATTATTTGGTCGAATGCTTTAAGAGAAAGAAGAAGCTTCCATTTGATCTTTACAAAGTTGAAGCTGTTGGTGAAACCTCTAGCATGGTCACTGTCAAAGTGAAAGGCCGAAGTGCCGTTCATGAATCTTCTGGATTGCAAGATACAGGTCACATTCTTGAGGATGGAAAAAGCATTTATAATACGACTTTAAGTATGTCTGATTTATCAACCGGTGTTAATAGTTATTACATCCTCCAAATTATACAAGATGATAAAAGTTCAGAGTGCCATGTGTTCCGTAAATGGGGACGAGTGGGGAATGACAAAATTGGAGGAACCAAACTGGACCAGATGTCAAAATATGATGCCATATCTGATTTTAAACGTTTGTTTCTTGAGAAGACTGGCAACTCATGGGAAGCATGGGAGCAAAAACAAGATTTTCAGAAGCAACCTGGCAAATTCTTTCCACTGGATATTGATTATGGTGTTAACAAAGAGGTCTCAAAGAAAAATCAGAACAACGCACCCAGCAAACTGCCTCCTCAGTTAGCAGAATTGATGAAGATGCTCTTCAATGTTGAAACATACAGAGCTGCCATGATGGAATTTGAGATAAATATGTCAGAAATGCCACTTGGAAAACTGAGTAAAAGCAATATCCAAAAGGGGTTTGAGGCATTAACGGAAATACAGAATCTATTAAAGAGTGATGGTGCCTCTTCCATTAAAGATAGCTTGATTGTAGATGCAAGCAATCGATTTTTCACTGTGATCCCTTCCATACATCCACATATTATTAGGGATGAGGATGACTTTAAGTCAAAGTTGAAAATGCTAGAAGCTCTCCAGGACATTGAAATTGCTTCCAGATTAGTCGGTTTTGATGCTGATAGTGATGACTCACTAGATGAGAAGTATAAGAAGCTCCGGTGTTGTATGAATCCACTTCCCCACGATAGTGAAGATTATCAGTTGATTGAAAAGTATCTTCTTACTACTCATGCCCCTACACATACGGATTGGAGTCTTGAACTTGAAGAAGTTTTTTCACTTGAAAGGGAAGGGGAATTTGATAAATACGCTCCCTACCGGAAAACGCTTAAAAACAGAATGCTGCTTTGGCATGGTTCTCGGTTTACGAATTTTGTGGGTATACTTAGCCAAGGACTAAGAATAGCTCCTCCTGAAGCTCCAGCTACTGGTTATATGTTTGGCAAGGGGATTTACTTTGCTGACCTTGTCAGTAAGAGTGCTCAGTATTGCTATACAGATAAGAAAAATCCTGTGGGCCTCATGCTTTTAAGTGAAGTTGCCCTGGGAGAGATCCATGAGCTCAAGAAGGCCACGTATATGGATAAACCTCCCAAGGGAAAGCACTCCACAAAGGGACTTGGCAAGAAAAAACCTGAGGAGTCGGACTACGTAAAATGGAGGGATGATGTTACTGTGCCGTGTGGCAAACCAGTGGACTCACATGTCAGGGCGTCTGAGCTGATGTATAATGAGTATATTGTTTATGATACAGCTCAAGTTAAGATGCAGTTTTTATTGAAGGTGAAGTTTCATCACAAGAGATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0003824 GO:0003909 GO:0003910 GO:0003950 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006266 GO:0006271 GO:0006273 GO:0006281 GO:0006464 GO:0006471 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006979 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009719 GO:0009725 GO:0009737 GO:0009987 GO:0010033 GO:0016070 GO:0016740 GO:0016757 GO:0016763 GO:0016874 GO:0016886 GO:0019538 GO:0022616 GO:0033554 GO:0033993 GO:0034641 GO:0034645 GO:0036211 GO:0042221 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0046483 GO:0050896 GO:0051103 GO:0051716 GO:0071704 GO:0090304 GO:0097305 GO:0140097 GO:1901360 GO:1901564 GO:1901576 GO:1901700

KEGG Pathways

Metabolic & Signaling

ko03410 ko04210 ko04212 ko04214 ko04217 map03410 map04210 map04212 map04214 map04217

Pfam Domains

Protein Families

BRCT PADR1 PARP PARP_reg WGR zf-PARP

Protein Analysis

958

Amino Acids

107.88

Weight (kDa)

8.83

Isoelectric Point (pI)

44.28

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
zf-PARP	PF00645	13 - 88	6.9e-22	Poly(ADP-ribose) polymerase and DNA-Ligase Zn-finger region
zf-PARP	PF00645	114 - 185	1.1e-11	Poly(ADP-ribose) polymerase and DNA-Ligase Zn-finger region
PADR1_N	PF21728	248 - 303	2.3e-19	PADR1, N-terminal helical domain
Zn_ribbon_PADR1	PF08063	304 - 343	6.5e-16	PADR1 domain, zinc ribbon fold
BRCT	PF00533	398 - 471	4.7e-08	BRCA1 C Terminus (BRCT) domain
WGR	PF05406	524 - 602	8.5e-17	WGR domain
PARP_reg	PF02877	636 - 765	4.3e-37	Poly(ADP-ribose) polymerase, regulatory domain
PARP	PF00644	779 - 908	1.3e-53	Poly(ADP-ribose) polymerase catalytic domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0000811)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G66290 AT5G66290
fragaria_vesca	FvH4_1g12000 FvH4_2g21980
malus_domestica	MD02G1134200.v1.1 MD15G1247200.v1.1
prunus_persica	Prupe.7G166600_v2.0.a1 Prupe.7G166600_v2.0.a1
pyrus_communis	pycom02g10580
rosa_chinensis	RchiOBHm_Chr0c39g0503221 RchiOBHm_Chr1g0353231 RchiOBHm_Chr2g0099641 RchiOBHm_Chr2g0126361 RchiOBHm_Chr5g0080231
rosa_laevigata	RLG00000008117 RLG00000016900 RLG00000021865
rosa_multiflora	Rmu_sc0000394.1_g000010 Rmu_sc0000543.1_g000026 Rmu_sc0005120.1_g000016 Rmu_sc0006325.1_g000056 Rmu_sc0010438.1_g000001 Rmu_sc0035741.1_g000001 Rmu_ssc0000366.1_g000005
rosa_roxburghii	Rroxscaffold_2G00142880 Rroxscaffold_3G00248750 Rroxscaffold_5G00378640 Rroxscaffold_5G00382790 Rroxscaffold_7G00190790
rosa_rugosa	Rorug02G0081700 Rorug02G0258300 Rorug06G0170500
rosa_samantha	Rh1CG034700 Rh1DG035400 Rh2AG131100 Rh2AG189600 Rh2AG189700 Rh2BG134500 Rh2BG230100 Rh2BG328200 Rh2CG135300 Rh2DG135900 Rh2DG196000 Rh2DG196100 Rh2DG196200 Rh3DG312100 Rh4CG305000 Rh4DG111800 Rh5AG369300 Rh5AG496200 Rh5AG496300 Rh5BG555300 Rh5CG578200 Rh5DG254100 Rh5DG254200 Rh6BG167500 Rh6BG167600 Rh6BG241100 Rh7AG282200 Rh7CG301400 Rh7CG301500 Rh7DG435700 Rh7DG435800
rosa_wichuraiana	Rw0G008970 Rw2G010150 Rw3G024850 Rw4G013560 Rw5G022800

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1491
AasI	GACNNNNNNGTC	1 cut(s)	2771
Acc36I	ACCTGC	1 cut(s)	1039
AccB1I	GGYRCC	2 cut(s)	453, 2009
AccB7I	CCANNNNNTGG	1 cut(s)	2495
AccI	GTMKAC	1 cut(s)	2449
AciI	CCGC	4 cut(s)	5, 103, 284, 605
AclI	AACGTT	1 cut(s)	1678
AclWI	GGATC	7 cut(s)	249, 595, 868, 1188, 1201, 2062, 2614
AcoI	YGGCCR	1 cut(s)	1087
AcsI	RAATTY	4 cut(s)	1754, 1901, 2363, 2437
AcuI	CTGAAG	3 cut(s)	756, 803, 2502
AcyI	GRCGYC	1 cut(s)	2780
AfaI	GTAC	2 cut(s)	41, 267
AfiI	CCNNNNNNNGG	4 cut(s)	238, 1597, 2495, 2663
AflII	CTTAAG	1 cut(s)	560
AflIII	ACRYGT	1 cut(s)	2770
AgeI	ACCGGT	1 cut(s)	1523
AhdI	GACNNNNNGTC	2 cut(s)	1645, 2113
AjnI	CCWGG	5 cut(s)	492, 1066, 1747, 2142, 2610
AjuI	GAANNNNNNNTTGG	4 cut(s)	1914, 1946, 2398, 2430
AleI	CACNNNNGTG	1 cut(s)	2253
AloI	GAACNNNNNNTCC	2 cut(s)	904, 936
Alw21I	GWGCWC	2 cut(s)	2551, 2631
Alw26I	GTCTC	2 cut(s)	512, 1801
AlwI	GGATC	7 cut(s)	249, 595, 868, 1188, 1201, 2062, 2614
AlwNI	CAGNNNCTG	3 cut(s)	375, 533, 2495
Ama87I	CYCGRG	1 cut(s)	357
AoxI	GGCC	7 cut(s)	123, 217, 327, 1087, 1417, 2584, 2637
ApeKI	GCWGC	5 cut(s)	226, 378, 542, 1889, 2410
ApoI	RAATTY	4 cut(s)	1754, 1901, 2363, 2437
AsiGI	ACCGGT	1 cut(s)	1523
AspS9I	GGNCC	6 cut(s)	7, 287, 296, 912, 1639, 2584
AsuHPI	GGTGA	5 cut(s)	474, 668, 704, 1394, 2863
AvaI	CYCGRG	1 cut(s)	357
AvaII	GGWCC	5 cut(s)	7, 287, 296, 912, 1639
AxyI	CCTNAGG	1 cut(s)	2703
BamHI	GGATCC	1 cut(s)	1193
BanI	GGYRCC	2 cut(s)	453, 2009
BanII	GRGCYC	2 cut(s)	781, 2631
BarI	GAAGNNNNNNTAC	2 cut(s)	23, 55
BbsI	GAAGAC	1 cut(s)	1700
Bbv12I	GWGCWC	2 cut(s)	2551, 2631
BbvI	GCAGC	5 cut(s)	213, 390, 554, 1876, 2397
BceAI	ACGGC	4 cut(s)	154, 1102, 1412, 2731
BcgI	CGANNNNNNTGC	4 cut(s)	45, 79, 2408, 2442
BciT130I	CCWGG	5 cut(s)	494, 1068, 1749, 2144, 2612
BciVI	GTATCC	1 cut(s)	301
BclI	TGATCA	1 cut(s)	535
BcoDI	GTCTC	2 cut(s)	512, 1801
BfaI	CTAG	5 cut(s)	438, 1101, 1392, 2132, 2201
BfmI	CTRYAG	2 cut(s)	376, 813
BfrI	CTTAAG	1 cut(s)	560
BfuAI	ACCTGC	1 cut(s)	1039
BfuI	GTATCC	1 cut(s)	301
BisI	GCNGC	5 cut(s)	227, 379, 543, 1890, 2411
BlsI	GCNGC	5 cut(s)	228, 380, 544, 1891, 2412
Bme1390I	CCNGG	5 cut(s)	494, 1068, 1749, 2144, 2612
Bme18I	GGWCC	5 cut(s)	7, 287, 296, 912, 1639
BmeRI	GACNNNNNGTC	2 cut(s)	1645, 2113
BmeT110I	CYCGRG	1 cut(s)	357
BmgT120I	GGNCC	6 cut(s)	7, 287, 296, 912, 1639, 2584
BmiI	GGNNCC	8 cut(s)	9, 158, 288, 455, 899, 1195, 1630, 2011
BmrFI	CCNGG	5 cut(s)	494, 1068, 1749, 2144, 2612
BmsI	GCATC	9 cut(s)	287, 810, 1243, 1252, 1648, 1851, 2032, 2170, 2823
BpiI	GAAGAC	1 cut(s)	1700
BplI	GAGNNNNNCTC	2 cut(s)	509, 541
BpmI	CTGGAG	3 cut(s)	1089, 2126, 2472
BpuEI	CTTGAG	6 cut(s)	242, 918, 1490, 1709, 2615, 2808
Bsa29I	ATCGAT	1 cut(s)	2053
BsaAI	YACGTR	2 cut(s)	2643, 2719
BsaHI	GRCGYC	1 cut(s)	2780
BsaI	GGTCTC	1 cut(s)	1801
BsaJI	CCNNGG	7 cut(s)	26, 492, 1155, 2458, 2610, 2611, 2661
BsaWI	WCCGGW	5 cut(s)	231, 1249, 1523, 2223, 2385
BsaXI	ACNNNNNCTCC	2 cut(s)	491, 521
Bsc4I	CCNNNNNNNGG	4 cut(s)	238, 1597, 2495, 2663
Bse118I	RCCGGY	1 cut(s)	1523
Bse1I	ACTGG	8 cut(s)	165, 330, 1195, 1641, 1702, 1770, 2500, 2759
Bse21I	CCTNAGG	1 cut(s)	2703
BseBI	CCWGG	5 cut(s)	494, 1068, 1749, 2144, 2612
BseCI	ATCGAT	1 cut(s)	2053
BseDI	CCNNGG	7 cut(s)	26, 492, 1155, 2458, 2610, 2611, 2661
BseLI	CCNNNNNNNGG	4 cut(s)	238, 1597, 2495, 2663
BseNI	ACTGG	8 cut(s)	165, 330, 1195, 1641, 1702, 1770, 2500, 2759
BseRI	GAGGAG	3 cut(s)	1827, 2466, 2720
BseXI	GCAGC	5 cut(s)	213, 390, 554, 1876, 2397
BseYI	CCCAGC	1 cut(s)	1823
BsgI	GTGCAG	1 cut(s)	1017
Bsh1285I	CGRYCG	1 cut(s)	59
BshFI	GGCC	7 cut(s)	125, 219, 329, 1089, 1419, 2586, 2639
BshNI	GGYRCC	2 cut(s)	453, 2009
BshTI	ACCGGT	1 cut(s)	1523
BshVI	ATCGAT	1 cut(s)	2053
BsiEI	CGRYCG	1 cut(s)	59
BsiHKAI	GWGCWC	2 cut(s)	2551, 2631
BsiHKCI	CYCGRG	1 cut(s)	357
BsiSI	CCGG	5 cut(s)	232, 1250, 1524, 2224, 2386
BslFI	GGGAC	3 cut(s)	953, 1614, 2697
BslI	CCNNNNNNNGG	4 cut(s)	238, 1597, 2495, 2663
BsmAI	GTCTC	2 cut(s)	512, 1801
BsmFI	GGGAC	3 cut(s)	953, 1614, 2697
BsmI	GAATGC	2 cut(s)	1331, 2412
BsnI	GGCC	7 cut(s)	125, 219, 329, 1089, 1419, 2586, 2639
Bso31I	GGTCTC	1 cut(s)	1801
BsoBI	CYCGRG	1 cut(s)	357
Bsp1286I	GDGCHC	3 cut(s)	781, 2551, 2631
Bsp1407I	TGTACA	1 cut(s)	265
Bsp19I	CCATGG	2 cut(s)	26, 1155
BspACI	CCGC	4 cut(s)	5, 103, 284, 605
BspANI	GGCC	7 cut(s)	125, 219, 329, 1089, 1419, 2586, 2639
BspDI	ATCGAT	1 cut(s)	2053
BspHI	TCATGA	1 cut(s)	1432
BspLI	GGNNCC	8 cut(s)	9, 158, 288, 455, 899, 1195, 1630, 2011
BspMAI	CTGCAG	1 cut(s)	380
BspMI	ACCTGC	1 cut(s)	1039
BspPI	GGATC	7 cut(s)	249, 595, 868, 1188, 1201, 2062, 2614
BspQI	GCTCTTC	1 cut(s)	1871
BspT107I	GGYRCC	2 cut(s)	453, 2009
BspTI	CTTAAG	1 cut(s)	560
BspTNI	GGTCTC	1 cut(s)	1801
BsrFI	RCCGGY	1 cut(s)	1523
BsrGI	TGTACA	1 cut(s)	265
BsrI	ACTGG	8 cut(s)	165, 330, 1195, 1641, 1702, 1770, 2500, 2759
BssAI	RCCGGY	1 cut(s)	1523
BssECI	CCNNGG	7 cut(s)	26, 492, 1155, 2458, 2610, 2611, 2661
BssNAI	GTATAC	1 cut(s)	2450
BssNI	GRCGYC	1 cut(s)	2780
BssT1I	CCWWGG	4 cut(s)	26, 1155, 2458, 2661
Bst1107I	GTATAC	1 cut(s)	2450
Bst2UI	CCWGG	5 cut(s)	494, 1068, 1749, 2144, 2612
Bst4CI	ACNGT	6 cut(s)	612, 814, 1064, 1405, 2065, 2743
Bst6I	CTCTTC	2 cut(s)	1871, 2020
BstACI	GRCGYC	1 cut(s)	2780
BstAFI	CTTAAG	1 cut(s)	560
BstAPI	GCANNNNNTGC	2 cut(s)	72, 375
BstAUI	TGTACA	1 cut(s)	265
BstBAI	YACGTR	2 cut(s)	2643, 2719
BstC8I	GCNNGC	4 cut(s)	371, 628, 1108, 2047
BstDSI	CCRYGG	2 cut(s)	26, 1155
BstMAI	GTCTC	2 cut(s)	512, 1801
BstMCI	CGRYCG	1 cut(s)	59
BstMWI	GCNNNNNNNGC	9 cut(s)	72, 290, 375, 551, 829, 874, 1112, 1262, 2416
BstNI	CCWGG	5 cut(s)	494, 1068, 1749, 2144, 2612
BstNSI	RCATGY	2 cut(s)	806, 2774
BstSCI	CCNGG	5 cut(s)	492, 1066, 1747, 2142, 2610
BstSFI	CTRYAG	2 cut(s)	376, 813
BstSNI	TACGTA	1 cut(s)	2719
BstV1I	GCAGC	5 cut(s)	213, 390, 554, 1876, 2397
BstV2I	GAAGAC	1 cut(s)	1700
BstX2I	RGATCY	3 cut(s)	600, 1193, 2619
BstXI	CCANNNNNNTGG	1 cut(s)	2647
BstYI	RGATCY	3 cut(s)	600, 1193, 2619
BstZ17I	GTATAC	1 cut(s)	2450
Bsu15I	ATCGAT	1 cut(s)	2053
Bsu36I	CCTNAGG	1 cut(s)	2703
BsuI	GTATCC	1 cut(s)	301
BsuRI	GGCC	7 cut(s)	125, 219, 329, 1089, 1419, 2586, 2639
BsuTUI	ATCGAT	1 cut(s)	2053
BtgI	CCRYGG	2 cut(s)	26, 1155
BtsIMutI	CAGTG	5 cut(s)	337, 1401, 1763, 2061, 2766
BveI	ACCTGC	1 cut(s)	1039
Cac8I	GCNNGC	4 cut(s)	371, 628, 1108, 2047
CaiI	CAGNNNCTG	3 cut(s)	375, 533, 2495
CciI	TCATGA	1 cut(s)	1432
Cfr10I	RCCGGY	1 cut(s)	1523
Cfr13I	GGNCC	6 cut(s)	7, 287, 296, 912, 1639, 2584
ClaI	ATCGAT	1 cut(s)	2053
CseI	GACGC	1 cut(s)	2769
Csp6I	GTAC	2 cut(s)	40, 266
CspAI	ACCGGT	1 cut(s)	1523
CspCI	CAANNNNNGTGG	2 cut(s)	313, 348
CviQI	GTAC	2 cut(s)	40, 266
DraI	TTTAAA	3 cut(s)	393, 1009, 1675
DrdI	GACNNNNNNGTC	1 cut(s)	2771
DriI	GACNNNNNGTC	2 cut(s)	1645, 2113
DseDI	GACNNNNNNGTC	1 cut(s)	2771
EaeI	YGGCCR	1 cut(s)	1087
Eam1104I	CTCTTC	2 cut(s)	1871, 2020
Eam1105I	GACNNNNNGTC	2 cut(s)	1645, 2113
EarI	CTCTTC	2 cut(s)	1871, 2020
EciI	GGCGGA	1 cut(s)	20
Ecl136II	GAGCTC	1 cut(s)	2629
Eco105I	TACGTA	1 cut(s)	2719
Eco130I	CCWWGG	4 cut(s)	26, 1155, 2458, 2661
Eco147I	AGGCCT	1 cut(s)	219
Eco24I	GRGCYC	2 cut(s)	781, 2631
Eco31I	GGTCTC	1 cut(s)	1801
Eco47I	GGWCC	5 cut(s)	7, 287, 296, 912, 1639
Eco53kI	GAGCTC	1 cut(s)	2629
Eco57I	CTGAAG	3 cut(s)	756, 803, 2502
Eco81I	CCTNAGG	1 cut(s)	2703
Eco88I	CYCGRG	1 cut(s)	357
EcoICRI	GAGCTC	1 cut(s)	2629
EcoO109I	RGGNCCY	1 cut(s)	287
EcoRII	CCWGG	5 cut(s)	492, 1066, 1747, 2142, 2610
EcoT14I	CCWWGG	4 cut(s)	26, 1155, 2458, 2661
EcoT38I	GRGCYC	2 cut(s)	781, 2631
ErhI	CCWWGG	4 cut(s)	26, 1155, 2458, 2661
FalI	AAGNNNNNCTT	2 cut(s)	1850, 1882
FaqI	GGGAC	3 cut(s)	953, 1614, 2697
FauI	CCCGC	2 cut(s)	277, 612
FbaI	TGATCA	1 cut(s)	535
FblI	GTMKAC	1 cut(s)	2449
Fnu4HI	GCNGC	5 cut(s)	227, 379, 543, 1890, 2411
FriOI	GRGCYC	2 cut(s)	781, 2631
Fsp4HI	GCNGC	5 cut(s)	227, 379, 543, 1890, 2411
FspBI	CTAG	5 cut(s)	438, 1101, 1392, 2132, 2201
GluI	GCNGC	5 cut(s)	227, 379, 543, 1890, 2411
GsaI	CCCAGC	1 cut(s)	1827
GsuI	CTGGAG	3 cut(s)	1089, 2126, 2472
HaeIII	GGCC	7 cut(s)	125, 219, 329, 1089, 1419, 2586, 2639
HapII	CCGG	5 cut(s)	232, 1250, 1524, 2224, 2386
HgaI	GACGC	1 cut(s)	2769
Hin1I	GRCGYC	1 cut(s)	2780
HincII	GTYRAC	3 cut(s)	615, 702, 1786
HindII	GTYRAC	3 cut(s)	615, 702, 1786
HindIII	AAGCTT	3 cut(s)	419, 1108, 1346
HpaI	GTTAAC	1 cut(s)	1786
HpaII	CCGG	5 cut(s)	232, 1250, 1524, 2224, 2386
HphI	GGTGA	5 cut(s)	474, 668, 704, 1394, 2863
Hpy166II	GTNNAC	6 cut(s)	615, 702, 1786, 2433, 2450, 2764
Hpy8I	GTNNAC	6 cut(s)	615, 702, 1786, 2433, 2450, 2764
Hpy99I	CGWCG	1 cut(s)	140
HpyCH4III	ACNGT	6 cut(s)	612, 814, 1064, 1405, 2065, 2743
HpyCH4IV	ACGT	4 cut(s)	946, 1678, 2642, 2718
HpyF10VI	GCNNNNNNNGC	9 cut(s)	72, 290, 375, 551, 829, 874, 1112, 1262, 2416
HpySE526I	ACGT	4 cut(s)	946, 1678, 2642, 2718
Hsp92I	GRCGYC	1 cut(s)	2780
Ksp22I	TGATCA	1 cut(s)	535
KspAI	GTTAAC	1 cut(s)	1786
LguI	GCTCTTC	1 cut(s)	1871
LmnI	GCTCC	7 cut(s)	234, 434, 1720, 2226, 2383, 2479, 2491
Lsp1109I	GCAGC	5 cut(s)	213, 390, 554, 1876, 2397
LweI	GCATC	9 cut(s)	287, 810, 1243, 1252, 1648, 1851, 2032, 2170, 2823
MaeI	CTAG	5 cut(s)	438, 1101, 1392, 2132, 2201
MaeII	ACGT	4 cut(s)	946, 1678, 2642, 2718
MaeIII	GTNAC	8 cut(s)	51, 656, 805, 878, 1186, 1399, 1460, 2737
MfeI	CAATTG	1 cut(s)	1299
MflI	RGATCY	3 cut(s)	600, 1193, 2619
MhlI	GDGCHC	3 cut(s)	781, 2551, 2631
MlyI	GAGTC	4 cut(s)	2189, 2329, 2717, 2759
MmeI	TCCRAC	2 cut(s)	666, 2691
MslI	CAYNNNNRTG	3 cut(s)	149, 2253, 2645
MspA1I	CMGCKG	2 cut(s)	284, 545
MspCI	CTTAAG	1 cut(s)	560
MspI	CCGG	5 cut(s)	232, 1250, 1524, 2224, 2386
MspR9I	CCNGG	5 cut(s)	494, 1068, 1749, 2144, 2612
MunI	CAATTG	1 cut(s)	1299
Mva1269I	GAATGC	2 cut(s)	1331, 2412
MvaI	CCWGG	5 cut(s)	494, 1068, 1749, 2144, 2612
MwoI	GCNNNNNNNGC	9 cut(s)	72, 290, 375, 551, 829, 874, 1112, 1262, 2416
NcoI	CCATGG	2 cut(s)	26, 1155
NlaIV	GGNNCC	8 cut(s)	9, 158, 288, 455, 899, 1195, 1630, 2011
NmuCI	GTSAC	6 cut(s)	51, 656, 805, 878, 1399, 1460
NspI	RCATGY	2 cut(s)	806, 2774
OliI	CACNNNNGTG	1 cut(s)	2253
PagI	TCATGA	1 cut(s)	1432
PasI	CCCWGGG	1 cut(s)	2611
PceI	AGGCCT	1 cut(s)	219
PciI	ACATGT	1 cut(s)	2770
PciSI	GCTCTTC	1 cut(s)	1871
PcsI	WCGNNNNNNNCGW	1 cut(s)	56
PctI	GAATGC	2 cut(s)	1331, 2412
PfeI	GAWTC	7 cut(s)	16, 476, 985, 1151, 1436, 1990, 2236
PflFI	GACNNNGTC	1 cut(s)	2534
PflMI	CCANNNNNTGG	1 cut(s)	2495
PfoI	TCCNGGA	2 cut(s)	1066, 2142
PinAI	ACCGGT	1 cut(s)	1523
PkrI	GCNGC	5 cut(s)	228, 380, 544, 1891, 2412
Ple19I	CGATCG	1 cut(s)	59
PleI	GAGTC	4 cut(s)	2189, 2328, 2716, 2759
PpsI	GAGTC	4 cut(s)	2189, 2328, 2716, 2759
Ppu21I	YACGTR	2 cut(s)	2643, 2719
PpuMI	RGGWCCY	1 cut(s)	287
PscI	ACATGT	1 cut(s)	2770
PsiI	TTATAA	1 cut(s)	1491
Psp124BI	GAGCTC	1 cut(s)	2631
Psp1406I	AACGTT	1 cut(s)	1678
Psp5II	RGGWCCY	1 cut(s)	287
Psp6I	CCWGG	5 cut(s)	492, 1066, 1747, 2142, 2610
PspFI	CCCAGC	1 cut(s)	1823
PspGI	CCWGG	5 cut(s)	492, 1066, 1747, 2142, 2610
PspN4I	GGNNCC	8 cut(s)	9, 158, 288, 455, 899, 1195, 1630, 2011
PspPI	GGNCC	6 cut(s)	7, 287, 296, 912, 1639, 2584
PspPPI	RGGWCCY	1 cut(s)	287
PstI	CTGCAG	1 cut(s)	380
PstNI	CAGNNNCTG	3 cut(s)	375, 533, 2495
PsuI	RGATCY	3 cut(s)	600, 1193, 2619
PsyI	GACNNNGTC	1 cut(s)	2534
PvuI	CGATCG	1 cut(s)	59
PvuII	CAGCTG	1 cut(s)	545
RsaI	GTAC	2 cut(s)	41, 267
RsaNI	GTAC	2 cut(s)	40, 266
RseI	CAYNNNNRTG	3 cut(s)	149, 2253, 2645
SacI	GAGCTC	1 cut(s)	2631
SapI	GCTCTTC	1 cut(s)	1871
SatI	GCNGC	5 cut(s)	227, 379, 543, 1890, 2411
Sau96I	GGNCC	6 cut(s)	7, 287, 296, 912, 1639, 2584
SchI	GAGTC	4 cut(s)	2189, 2329, 2717, 2759
ScrFI	CCNGG	5 cut(s)	494, 1068, 1749, 2144, 2612
SduI	GDGCHC	3 cut(s)	781, 2551, 2631
SfaNI	GCATC	9 cut(s)	287, 810, 1243, 1252, 1648, 1851, 2032, 2170, 2823
SfcI	CTRYAG	2 cut(s)	376, 813
SinI	GGWCC	5 cut(s)	7, 287, 296, 912, 1639
SmiMI	CAYNNNNRTG	3 cut(s)	149, 2253, 2645
SmlI	CTYRAG	7 cut(s)	221, 560, 933, 1469, 1688, 2630, 2823
SmoI	CTYRAG	7 cut(s)	221, 560, 933, 1469, 1688, 2630, 2823
SnaBI	TACGTA	1 cut(s)	2719
SseBI	AGGCCT	1 cut(s)	219
SsiI	CCGC	4 cut(s)	5, 103, 284, 605
SspMI	CTAG	5 cut(s)	438, 1101, 1392, 2132, 2201
SstI	GAGCTC	1 cut(s)	2631
StuI	AGGCCT	1 cut(s)	219
StyD4I	CCNGG	5 cut(s)	492, 1066, 1747, 2142, 2610
StyI	CCWWGG	4 cut(s)	26, 1155, 2458, 2661
TaaI	ACNGT	6 cut(s)	612, 814, 1064, 1405, 2065, 2743
TaiI	ACGT	4 cut(s)	949, 1681, 2645, 2721
TaqI	TCGA	4 cut(s)	36, 135, 1323, 2053
TatI	WGTACW	1 cut(s)	265
TfiI	GAWTC	7 cut(s)	16, 476, 985, 1151, 1436, 1990, 2236
TscAI	CASTG	5 cut(s)	337, 1408, 1770, 2068, 2766
TseFI	GTSAC	6 cut(s)	51, 656, 805, 878, 1399, 1460
TseI	GCWGC	5 cut(s)	226, 378, 542, 1889, 2410
Tsp45I	GTSAC	6 cut(s)	51, 656, 805, 878, 1399, 1460
TspGWI	ACGGA	4 cut(s)	1186, 1580, 1994, 2327
TspRI	CASTG	5 cut(s)	337, 1408, 1770, 2068, 2766
Tth111I	GACNNNGTC	1 cut(s)	2534
Van91I	CCANNNNNTGG	1 cut(s)	2495
Vha464I	CTTAAG	1 cut(s)	560
VpaK11BI	GGWCC	5 cut(s)	7, 287, 296, 912, 1639
XapI	RAATTY	4 cut(s)	1754, 1901, 2363, 2437
XceI	RCATGY	2 cut(s)	806, 2774
XcmI	CCANNNNNNNNNTGG	1 cut(s)	469
XmiI	GTMKAC	1 cut(s)	2449
XspI	CTAG	5 cut(s)	438, 1101, 1392, 2132, 2201

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.