FvH4_1g12000

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0000811
Physical Location & Seq
Reverse (-)
6562247 .. 6564569
2323 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g12000.t1

Sequence Viewer

Length: 639 bp
ATGGGTGCCACAGAATCCTCACTCTCGAGCTCACAGAGGCGGCCGGCCGATGAAATAACCACCGTGTCGGAGCGATCGGAGGTCGCCGATCCTATACTAGAGAGGCTGAAGTCGCTAAGAATTTCGACGCCGTTATTGACTTCACCTCCAAGTGAGGGTAGCTTGACTGACATTTTAGTGAGGAAGCCTTCGTCTTCTTCAGATCCCAGTATTGTGGATCCGAAAGTGCTATTCGAACTCTTTGCGATGTACCGCGAGTGGCAAGAGGAGAATGTCAAAAGGCTGAGCCAAAAACAGGAAGAGATAGAGAATAAGATAGAAATTGCGGACGCGTTGGCTGCTAAACTTCAACAACGTTTTAATTATTCAGTGACGGCAATGAAGACATCCTCACAACATTTATCTGGAGTTTATGCATTGCAGGTAGAGCTTGGGGAACTTAAAGGAAGGTTGACGGAGGTTATTAGCAACTGCGATGCAGTGTGCAAGAGAATATCTGCCGAGGGACCCGAATCTCTTCGGTCATCTGTTAAACCGTTAGTTATCTGCACCGCACAGCAAGTAGACCAATCTAACTCATCTGATATGCAAAGAGATCCAACCCCAAGTCTACCTTCTGCCGAAACCAAGTTAGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

213

Amino Acids

23.35

Weight (kDa)

5.06

Isoelectric Point (pI)

66.45

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 412
AccB1I GGYRCC 1 cut(s) 5
AccI GTMKAC 2 cut(s) 564, 610
AccII CGCG 2 cut(s) 255, 332
AciI CCGC 4 cut(s) 40, 253, 326, 552
AclI AACGTT 1 cut(s) 355
AclWI GGATC 5 cut(s) 83, 197, 212, 225, 590
AcoI YGGCCR 2 cut(s) 41, 45
AcsI RAATTY 1 cut(s) 120
AcuI CTGAAG 2 cut(s) 128, 183
AcyI GRCGYC 1 cut(s) 128
AfaI GTAC 1 cut(s) 251
AfiI CCNNNNNNNGG 2 cut(s) 155, 295
AflIII ACRYGT 1 cut(s) 330
AgsI TTSAA 1 cut(s) 350
AjuI GAANNNNNNNTTGG 2 cut(s) 598, 630
AluBI AGCT 3 cut(s) 30, 162, 430
AluI AGCT 3 cut(s) 30, 162, 430
Alw21I GWGCWC 1 cut(s) 32
AlwI GGATC 5 cut(s) 83, 197, 212, 225, 590
Ama87I CYCGRG 1 cut(s) 25
AoxI GGCC 2 cut(s) 41, 45
ApeKI GCWGC 1 cut(s) 338
ApoI RAATTY 1 cut(s) 120
Asp700I GAANNNNTTC 1 cut(s) 516
AspS9I GGNCC 1 cut(s) 506
AsuHPI GGTGA 1 cut(s) 135
AsuII TTCGAA 1 cut(s) 234
AvaI CYCGRG 1 cut(s) 25
AvaII GGWCC 1 cut(s) 506
BamHI GGATCC 1 cut(s) 217
BanI GGYRCC 1 cut(s) 5
BanII GRGCYC 1 cut(s) 32
BbsI GAAGAC 2 cut(s) 186, 389
Bbv12I GWGCWC 1 cut(s) 32
BbvI GCAGC 1 cut(s) 325
BceAI ACGGC 2 cut(s) 115, 390
BcgI CGANNNNNNTGC 2 cut(s) 224, 258
BfaI CTAG 1 cut(s) 98
BfuAI ACCTGC 1 cut(s) 412
BisI GCNGC 2 cut(s) 41, 339
BlpI GCTNAGC 1 cut(s) 284
BlsI GCNGC 2 cut(s) 42, 340
Bme18I GGWCC 1 cut(s) 506
BmeT110I CYCGRG 1 cut(s) 25
BmgT120I GGNCC 1 cut(s) 506
BmiI GGNNCC 4 cut(s) 7, 219, 507, 508
BmrI ACTGGG 1 cut(s) 201
BmsI GCATC 1 cut(s) 466
BmuI ACTGGG 1 cut(s) 201
BpiI GAAGAC 2 cut(s) 186, 389
BpmI CTGGAG 1 cut(s) 426
Bpu1102I GCTNAGC 1 cut(s) 284
Bpu14I TTCGAA 1 cut(s) 234
BsaHI GRCGYC 1 cut(s) 128
BsaJI CCNNGG 1 cut(s) 501
BsaXI ACNNNNNCTCC 2 cut(s) 130, 160
Bsc4I CCNNNNNNNGG 2 cut(s) 155, 295
Bse118I RCCGGY 1 cut(s) 43
Bse1I ACTGG 1 cut(s) 207
Bse3DI GCAATG 2 cut(s) 384, 416
BseDI CCNNGG 1 cut(s) 501
BseGI GGATG 1 cut(s) 386
BseLI CCNNNNNNNGG 2 cut(s) 155, 295
BseMI GCAATG 2 cut(s) 384, 416
BseMII CTCAG 1 cut(s) 275
BseNI ACTGG 1 cut(s) 207
BseRI GAGGAG 1 cut(s) 281
BseX3I CGGCCG 2 cut(s) 41, 45
BseXI GCAGC 1 cut(s) 325
BsgI GTGCAG 1 cut(s) 532
Bsh1236I CGCG 2 cut(s) 255, 332
Bsh1285I CGRYCG 3 cut(s) 44, 48, 77
BshFI GGCC 2 cut(s) 43, 47
BshNI GGYRCC 1 cut(s) 5
BsiEI CGRYCG 3 cut(s) 44, 48, 77
BsiHKAI GWGCWC 1 cut(s) 32
BsiHKCI CYCGRG 1 cut(s) 25
BsiSI CCGG 1 cut(s) 44
BslFI GGGAC 1 cut(s) 519
BslI CCNNNNNNNGG 2 cut(s) 155, 295
BsmFI GGGAC 1 cut(s) 519
BsnI GGCC 2 cut(s) 43, 47
BsoBI CYCGRG 1 cut(s) 25
Bsp119I TTCGAA 1 cut(s) 234
Bsp1286I GDGCHC 1 cut(s) 32
Bsp143I GATC 5 cut(s) 74, 88, 202, 217, 595
Bsp1720I GCTNAGC 1 cut(s) 284
BspACI CCGC 4 cut(s) 40, 253, 326, 552
BspANI GGCC 2 cut(s) 43, 47
BspCNI CTCAG 1 cut(s) 276
BspFNI CGCG 2 cut(s) 255, 332
BspLI GGNNCC 4 cut(s) 7, 219, 507, 508
BspMI ACCTGC 1 cut(s) 412
BspPI GGATC 5 cut(s) 83, 197, 212, 225, 590
BspT104I TTCGAA 1 cut(s) 234
BspT107I GGYRCC 1 cut(s) 5
BsrDI GCAATG 2 cut(s) 384, 416
BsrFI RCCGGY 1 cut(s) 43
BsrI ACTGG 1 cut(s) 207
BssAI RCCGGY 1 cut(s) 43
BssECI CCNNGG 1 cut(s) 501
BssMI GATC 5 cut(s) 74, 88, 202, 217, 595
BssNI GRCGYC 1 cut(s) 128
Bst4CI ACNGT 2 cut(s) 64, 537
Bst6I CTCTTC 2 cut(s) 294, 522
BstACI GRCGYC 1 cut(s) 128
BstBI TTCGAA 1 cut(s) 234
BstC8I GCNNGC 1 cut(s) 45
BstDEI CTNAG 2 cut(s) 116, 284
BstF5I GGATG 1 cut(s) 386
BstFNI CGCG 2 cut(s) 255, 332
BstKTI GATC 5 cut(s) 77, 91, 205, 220, 598
BstMBI GATC 5 cut(s) 74, 88, 202, 217, 595
BstMCI CGRYCG 3 cut(s) 44, 48, 77
BstMWI GCNNNNNNNGC 3 cut(s) 112, 338, 427
BstUI CGCG 2 cut(s) 255, 332
BstV1I GCAGC 1 cut(s) 325
BstV2I GAAGAC 2 cut(s) 186, 389
BstX2I RGATCY 3 cut(s) 202, 217, 595
BstXI CCANNNNNNTGG 1 cut(s) 214
BstYI RGATCY 3 cut(s) 202, 217, 595
BstZI CGGCCG 2 cut(s) 41, 45
BsuRI GGCC 2 cut(s) 43, 47
BtgZI GCGATG 2 cut(s) 260, 489
BtsCI GGATG 1 cut(s) 386
BtsI GCAGTG 1 cut(s) 486
BtsIMutI CAGTG 2 cut(s) 375, 486
BveI ACCTGC 1 cut(s) 412
Cac8I GCNNGC 1 cut(s) 45
Cfr10I RCCGGY 1 cut(s) 43
Cfr13I GGNCC 1 cut(s) 506
CseI GACGC 2 cut(s) 136, 338
Csp6I GTAC 1 cut(s) 250
CviQI GTAC 1 cut(s) 250
DdeI CTNAG 2 cut(s) 116, 284
DpnI GATC 5 cut(s) 76, 90, 204, 219, 597
DpnII GATC 5 cut(s) 74, 88, 202, 217, 595
EaeI YGGCCR 2 cut(s) 41, 45
EagI CGGCCG 2 cut(s) 41, 45
Eam1104I CTCTTC 2 cut(s) 294, 522
EarI CTCTTC 2 cut(s) 294, 522
Ecl136II GAGCTC 1 cut(s) 30
EclXI CGGCCG 2 cut(s) 41, 45
Eco24I GRGCYC 1 cut(s) 32
Eco47I GGWCC 1 cut(s) 506
Eco52I CGGCCG 2 cut(s) 41, 45
Eco53kI GAGCTC 1 cut(s) 30
Eco57I CTGAAG 2 cut(s) 128, 183
Eco88I CYCGRG 1 cut(s) 25
EcoICRI GAGCTC 1 cut(s) 30
EcoO109I RGGNCCY 1 cut(s) 506
EcoT22I ATGCAT 1 cut(s) 418
EcoT38I GRGCYC 1 cut(s) 32
FaiI YATR 3 cut(s) 95, 414, 587
FalI AAGNNNNNCTT 2 cut(s) 598, 630
FaqI GGGAC 1 cut(s) 519
FblI GTMKAC 2 cut(s) 564, 610
Fnu4HI GCNGC 2 cut(s) 41, 339
FokI GGATG 1 cut(s) 373
FriOI GRGCYC 1 cut(s) 32
FseI GGCCGGCC 1 cut(s) 47
Fsp4HI GCNGC 2 cut(s) 41, 339
FspBI CTAG 1 cut(s) 98
GluI GCNGC 2 cut(s) 41, 339
GsuI CTGGAG 1 cut(s) 426
HaeIII GGCC 2 cut(s) 43, 47
HapII CCGG 1 cut(s) 44
HgaI GACGC 2 cut(s) 136, 338
Hin1I GRCGYC 1 cut(s) 128
HincII GTYRAC 1 cut(s) 453
HindII GTYRAC 1 cut(s) 453
HinfI GANTC 2 cut(s) 14, 512
HpaII CCGG 1 cut(s) 44
HphI GGTGA 1 cut(s) 135
Hpy166II GTNNAC 3 cut(s) 453, 565, 611
Hpy188I TCNGA 5 cut(s) 70, 79, 202, 222, 583
Hpy188III TCNNGA 2 cut(s) 25, 405
Hpy8I GTNNAC 3 cut(s) 453, 565, 611
Hpy99I CGWCG 1 cut(s) 130
HpyAV CCTTC 3 cut(s) 198, 441, 624
HpyCH4III ACNGT 2 cut(s) 64, 537
HpyCH4IV ACGT 1 cut(s) 355
HpyCH4V TGCA 6 cut(s) 416, 421, 479, 486, 549, 589
HpyF10VI GCNNNNNNNGC 3 cut(s) 112, 338, 427
HpyF3I CTNAG 2 cut(s) 116, 284
HpySE526I ACGT 1 cut(s) 355
Hsp92I GRCGYC 1 cut(s) 128
KflI GGGWCCC 1 cut(s) 506
KroI GCCGGC 1 cut(s) 43
KroNI GCCGGC 1 cut(s) 45
Kzo9I GATC 5 cut(s) 74, 88, 202, 217, 595
LmnI GCTCC 1 cut(s) 70
LpnPI CCDG 5 cut(s) 57, 220, 281, 390, 407
Lsp1109I GCAGC 1 cut(s) 325
LweI GCATC 1 cut(s) 466
MaeI CTAG 1 cut(s) 98
MaeII ACGT 1 cut(s) 355
MaeIII GTNAC 1 cut(s) 370
MalI GATC 5 cut(s) 76, 90, 204, 219, 597
MboI GATC 5 cut(s) 74, 88, 202, 217, 595
MboII GAAGA 5 cut(s) 186, 189, 311, 394, 509
MflI RGATCY 3 cut(s) 202, 217, 595
MhlI GDGCHC 1 cut(s) 32
MluCI AATT 3 cut(s) 120, 321, 361
MluI ACGCGT 1 cut(s) 330
MmeI TCCRAC 2 cut(s) 48, 623
Mph1103I ATGCAT 1 cut(s) 418
MroNI GCCGGC 1 cut(s) 43
MroXI GAANNNNTTC 1 cut(s) 516
MseI TTAA 3 cut(s) 360, 441, 531
MslI CAYNNNNRTG 1 cut(s) 176
MspI CCGG 1 cut(s) 44
MvnI CGCG 2 cut(s) 255, 332
MwoI GCNNNNNNNGC 3 cut(s) 112, 338, 427
NaeI GCCGGC 1 cut(s) 45
NdeII GATC 5 cut(s) 74, 88, 202, 217, 595
NgoMIV GCCGGC 1 cut(s) 43
NlaIV GGNNCC 4 cut(s) 7, 219, 507, 508
NmeAIII GCCGAG 1 cut(s) 526
NmuCI GTSAC 1 cut(s) 370
NsiI ATGCAT 1 cut(s) 418
NspV TTCGAA 1 cut(s) 234
PaeR7I CTCGAG 1 cut(s) 25
PdiI GCCGGC 1 cut(s) 45
PdmI GAANNNNTTC 1 cut(s) 516
PfeI GAWTC 2 cut(s) 14, 512
PkrI GCNGC 2 cut(s) 42, 340
Ple19I CGATCG 1 cut(s) 77
PpuMI RGGWCCY 1 cut(s) 506
Psp124BI GAGCTC 1 cut(s) 32
Psp1406I AACGTT 1 cut(s) 355
Psp5II RGGWCCY 1 cut(s) 506
PspN4I GGNNCC 4 cut(s) 7, 219, 507, 508
PspPI GGNCC 1 cut(s) 506
PspPPI RGGWCCY 1 cut(s) 506
PsuI RGATCY 3 cut(s) 202, 217, 595
PvuI CGATCG 1 cut(s) 77
RigI GGCCGGCC 1 cut(s) 47
RsaI GTAC 1 cut(s) 251
RsaNI GTAC 1 cut(s) 250
RseI CAYNNNNRTG 1 cut(s) 176
SacI GAGCTC 1 cut(s) 32
SaqAI TTAA 3 cut(s) 360, 441, 531
SatI GCNGC 2 cut(s) 41, 339
Sau3AI GATC 5 cut(s) 74, 88, 202, 217, 595
Sau96I GGNCC 1 cut(s) 506
SduI GDGCHC 1 cut(s) 32
SfaNI GCATC 1 cut(s) 466
Sfr274I CTCGAG 1 cut(s) 25
SfuI TTCGAA 1 cut(s) 234
SinI GGWCC 1 cut(s) 506
SlaI CTCGAG 1 cut(s) 25
SmiMI CAYNNNNRTG 1 cut(s) 176
SmlI CTYRAG 1 cut(s) 25
SmoI CTYRAG 1 cut(s) 25
Sse9I AATT 3 cut(s) 120, 321, 361
SsiI CCGC 4 cut(s) 40, 253, 326, 552
SspMI CTAG 1 cut(s) 98
SstI GAGCTC 1 cut(s) 32
TaaI ACNGT 2 cut(s) 64, 537
TaiI ACGT 1 cut(s) 358
TaqI TCGA 3 cut(s) 26, 125, 234
TaqII GACCGA 1 cut(s) 510
TasI AATT 3 cut(s) 120, 321, 361
TauI GCSGC 1 cut(s) 43
TfiI GAWTC 2 cut(s) 14, 512
Tru1I TTAA 3 cut(s) 360, 441, 531
Tru9I TTAA 3 cut(s) 360, 441, 531
TscAI CASTG 2 cut(s) 375, 486
TseFI GTSAC 1 cut(s) 370
TseI GCWGC 1 cut(s) 338
Tsp45I GTSAC 1 cut(s) 370
TspDTI ATGAA 2 cut(s) 66, 395
TspGWI ACGGA 1 cut(s) 470
TspRI CASTG 2 cut(s) 375, 486
VpaK11BI GGWCC 1 cut(s) 506
XapI RAATTY 1 cut(s) 120
XhoI CTCGAG 1 cut(s) 25
XmiI GTMKAC 2 cut(s) 564, 610
XmnI GAANNNNTTC 1 cut(s) 516
XspI CTAG 1 cut(s) 98
Zsp2I ATGCAT 1 cut(s) 418
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.