MD15G1247200.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0000811
Physical Location & Seq
Reverse (-)
20517663 .. 20520082
2420 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1247200.v1.1.491

Sequence Viewer

Length: 642 bp
ATGGGCGCCTCAGAATCCACACTCTCAAGCTCACAGAGGCCGGCCGAAGAAATCACCACCGTGTCGGAACGATCAGAGACCGCCGATCCCGTCTTAGAGAAGCTCAAATCCCTCAAAATCTCGACGCCCCTGCTGACTTCGCCCCCGGCTGAGGGCAGCTTGACTGACATTTTAGTGAGGAAGCCTTCGTCTTCTTCAGTGCCAAGTGTTGTGGATCCGAAAGTGCTATTGGAACTCTTCTCAATGTACCGTGAGTGGCAGGAGGATAATGTCCAAAAGATAAGCAAAAGACAGGGAGAGGTAGAAAATAAAATAGAGGTTACGGATGCTTTGGCTGCTAAACTTCTTCAACGTTTTAATTATTCAGTGTCGGCAATGAAGACATCTTCACAACATTTATCCGAAGTTCATTCGTTGCAGGTGGAGATTGGGGAGCTTAAAGGAAGGTTAACAGAGGTCATTAGCAACTGTGATGCACTGTGCCGGAGAATATCTGTTGAAGGACCCGAATCCCTCCGGTCATCCATCAAGCCGTTTGCAGTCTGCACCACCGGCCAAGAAATCCAATCTAGCTCTAGCTCATCCGACGTGCAAAGAGATCCAGACCAAAGTCTACCATCCGCAGCATCCAAGTTAGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

214

Amino Acids

23.18

Weight (kDa)

5.15

Isoelectric Point (pI)

62.85

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 409
Acc36I ACCTGC 1 cut(s) 409
AccB1I GGYRCC 1 cut(s) 5
AccI GTMKAC 1 cut(s) 613
AciI CCGC 2 cut(s) 81, 621
AclI AACGTT 1 cut(s) 352
AclWI GGATC 4 cut(s) 80, 209, 222, 593
AcoI YGGCCR 2 cut(s) 42, 553
AcuI CTGAAG 1 cut(s) 180
AcyI GRCGYC 2 cut(s) 6, 125
AfaI GTAC 1 cut(s) 248
AfiI CCNNNNNNNGG 2 cut(s) 151, 152
AgsI TTSAA 2 cut(s) 350, 500
AjiI CACGTC 1 cut(s) 589
AjuI GAANNNNNNNTTGG 2 cut(s) 212, 244
AleI CACNNNNGTG 1 cut(s) 59
AluBI AGCT 6 cut(s) 30, 103, 159, 436, 573, 579
AluI AGCT 6 cut(s) 30, 103, 159, 436, 573, 579
Alw26I GTCTC 1 cut(s) 71
AlwI GGATC 4 cut(s) 80, 209, 222, 593
AoxI GGCC 3 cut(s) 38, 42, 553
ApeKI GCWGC 3 cut(s) 156, 335, 623
AspLEI GCGC 1 cut(s) 8
AspS9I GGNCC 1 cut(s) 503
AsuC2I CCSGG 1 cut(s) 146
AsuHPI GGTGA 1 cut(s) 46
AvaII GGWCC 1 cut(s) 503
BamHI GGATCC 1 cut(s) 214
BanI GGYRCC 1 cut(s) 5
BbsI GAAGAC 2 cut(s) 183, 386
BbvCI CCTCAGC 1 cut(s) 150
BbvI GCAGC 3 cut(s) 168, 322, 635
BccI CCATC 2 cut(s) 533, 625
BceAI ACGGC 1 cut(s) 517
BcgI CGANNNNNNTGC 2 cut(s) 112, 146
BcnI CCSGG 1 cut(s) 146
BcoDI GTCTC 1 cut(s) 71
BfaI CTAG 2 cut(s) 570, 576
BfoI RGCGCY 1 cut(s) 9
BfuAI ACCTGC 1 cut(s) 409
BisI GCNGC 3 cut(s) 157, 336, 624
BlsI GCNGC 3 cut(s) 158, 337, 625
Bme1390I CCNGG 1 cut(s) 146
Bme18I GGWCC 1 cut(s) 503
BmgBI CACGTC 1 cut(s) 589
BmgT120I GGNCC 1 cut(s) 503
BmiI GGNNCC 3 cut(s) 7, 216, 505
BmrFI CCNGG 1 cut(s) 146
BmsI GCATC 3 cut(s) 316, 463, 635
BoxI GACNNNNGTC 1 cut(s) 609
BpiI GAAGAC 2 cut(s) 183, 386
Bpu10I CCTNAGC 1 cut(s) 150
BpuEI CTTGAG 1 cut(s) 10
BpuMI CCSGG 1 cut(s) 146
BsaHI GRCGYC 2 cut(s) 6, 125
BsaI GGTCTC 1 cut(s) 71
BsaJI CCNNGG 1 cut(s) 144
BsaWI WCCGGW 1 cut(s) 516
Bsc4I CCNNNNNNNGG 2 cut(s) 151, 152
Bse118I RCCGGY 2 cut(s) 40, 551
Bse3DI GCAATG 1 cut(s) 381
BseDI CCNNGG 1 cut(s) 144
BseGI GGATG 5 cut(s) 331, 521, 581, 617, 626
BseLI CCNNNNNNNGG 2 cut(s) 151, 152
BseMI GCAATG 1 cut(s) 381
BseMII CTCAG 2 cut(s) 24, 141
BseX3I CGGCCG 1 cut(s) 42
BseXI GCAGC 3 cut(s) 168, 322, 635
BsgI GTGCAG 1 cut(s) 529
Bsh1285I CGRYCG 1 cut(s) 45
BshFI GGCC 3 cut(s) 40, 44, 555
BshNI GGYRCC 1 cut(s) 5
BsiEI CGRYCG 1 cut(s) 45
BsiSI CCGG 5 cut(s) 41, 146, 484, 517, 552
BslI CCNNNNNNNGG 2 cut(s) 151, 152
BsmAI GTCTC 1 cut(s) 71
BsnI GGCC 3 cut(s) 40, 44, 555
Bso31I GGTCTC 1 cut(s) 71
Bsp143I GATC 4 cut(s) 71, 85, 214, 598
BspACI CCGC 2 cut(s) 81, 621
BspANI GGCC 3 cut(s) 40, 44, 555
BspCNI CTCAG 2 cut(s) 23, 142
BspLI GGNNCC 3 cut(s) 7, 216, 505
BspMI ACCTGC 1 cut(s) 409
BspPI GGATC 4 cut(s) 80, 209, 222, 593
BspT107I GGYRCC 1 cut(s) 5
BspTNI GGTCTC 1 cut(s) 71
BsrDI GCAATG 1 cut(s) 381
BsrFI RCCGGY 2 cut(s) 40, 551
BssAI RCCGGY 2 cut(s) 40, 551
BssECI CCNNGG 1 cut(s) 144
BssMI GATC 4 cut(s) 71, 85, 214, 598
BssNI GRCGYC 2 cut(s) 6, 125
Bst4CI ACNGT 4 cut(s) 61, 251, 470, 480
Bst6I CTCTTC 1 cut(s) 242
BstACI GRCGYC 2 cut(s) 6, 125
BstC8I GCNNGC 1 cut(s) 42
BstDEI CTNAG 3 cut(s) 10, 94, 150
BstF5I GGATG 5 cut(s) 331, 521, 581, 617, 626
BstH2I RGCGCY 1 cut(s) 9
BstHHI GCGC 1 cut(s) 8
BstKTI GATC 4 cut(s) 74, 88, 217, 601
BstMAI GTCTC 1 cut(s) 71
BstMBI GATC 4 cut(s) 71, 85, 214, 598
BstMCI CGRYCG 1 cut(s) 45
BstMWI GCNNNNNNNGC 3 cut(s) 139, 335, 552
BstPAI GACNNNNGTC 1 cut(s) 609
BstSCI CCNGG 1 cut(s) 144
BstV1I GCAGC 3 cut(s) 168, 322, 635
BstV2I GAAGAC 2 cut(s) 183, 386
BstX2I RGATCY 2 cut(s) 214, 598
BstYI RGATCY 2 cut(s) 214, 598
BstZI CGGCCG 1 cut(s) 42
BsuRI GGCC 3 cut(s) 40, 44, 555
BtrI CACGTC 1 cut(s) 589
BtsCI GGATG 5 cut(s) 331, 521, 581, 617, 626
BtsIMutI CAGTG 3 cut(s) 204, 372, 476
BveI ACCTGC 1 cut(s) 409
Cac8I GCNNGC 1 cut(s) 42
CfoI GCGC 1 cut(s) 8
Cfr10I RCCGGY 2 cut(s) 40, 551
Cfr13I GGNCC 1 cut(s) 503
CseI GACGC 1 cut(s) 133
Csp6I GTAC 1 cut(s) 247
CspCI CAANNNNNGTGG 2 cut(s) 192, 227
CviQI GTAC 1 cut(s) 247
DdeI CTNAG 3 cut(s) 10, 94, 150
DinI GGCGCC 1 cut(s) 7
DpnI GATC 4 cut(s) 73, 87, 216, 600
DpnII GATC 4 cut(s) 71, 85, 214, 598
EaeI YGGCCR 2 cut(s) 42, 553
EagI CGGCCG 1 cut(s) 42
Eam1104I CTCTTC 1 cut(s) 242
EarI CTCTTC 1 cut(s) 242
EclXI CGGCCG 1 cut(s) 42
Eco31I GGTCTC 1 cut(s) 71
Eco47I GGWCC 1 cut(s) 503
Eco52I CGGCCG 1 cut(s) 42
Eco57I CTGAAG 1 cut(s) 180
EcoO109I RGGNCCY 1 cut(s) 503
EgeI GGCGCC 1 cut(s) 7
EheI GGCGCC 1 cut(s) 7
FblI GTMKAC 1 cut(s) 613
Fnu4HI GCNGC 3 cut(s) 157, 336, 624
FokI GGATG 5 cut(s) 338, 508, 568, 604, 613
FseI GGCCGGCC 1 cut(s) 44
Fsp4HI GCNGC 3 cut(s) 157, 336, 624
FspBI CTAG 2 cut(s) 570, 576
GlaI GCGC 1 cut(s) 7
GluI GCNGC 3 cut(s) 157, 336, 624
HaeII RGCGCY 1 cut(s) 9
HaeIII GGCC 3 cut(s) 40, 44, 555
HapII CCGG 5 cut(s) 41, 146, 484, 517, 552
HgaI GACGC 1 cut(s) 133
HhaI GCGC 1 cut(s) 8
Hin1I GRCGYC 2 cut(s) 6, 125
Hin6I GCGC 1 cut(s) 6
HinP1I GCGC 1 cut(s) 6
HincII GTYRAC 1 cut(s) 450
HindII GTYRAC 1 cut(s) 450
HinfI GANTC 2 cut(s) 14, 509
HpaI GTTAAC 1 cut(s) 450
HpaII CCGG 5 cut(s) 41, 146, 484, 517, 552
HphI GGTGA 1 cut(s) 46
Hpy166II GTNNAC 2 cut(s) 450, 614
Hpy188I TCNGA 6 cut(s) 13, 67, 76, 219, 403, 586
Hpy188III TCNNGA 2 cut(s) 121, 602
Hpy8I GTNNAC 2 cut(s) 450, 614
Hpy99I CGWCG 2 cut(s) 127, 590
HpyAV CCTTC 3 cut(s) 195, 438, 494
HpyCH4III ACNGT 4 cut(s) 61, 251, 470, 480
HpyCH4IV ACGT 2 cut(s) 352, 588
HpyCH4V TGCA 5 cut(s) 418, 476, 539, 546, 592
HpyF10VI GCNNNNNNNGC 3 cut(s) 139, 335, 552
HpyF3I CTNAG 3 cut(s) 10, 94, 150
HpySE526I ACGT 2 cut(s) 352, 588
Hsp92I GRCGYC 2 cut(s) 6, 125
HspAI GCGC 1 cut(s) 6
KasI GGCGCC 1 cut(s) 5
KroI GCCGGC 1 cut(s) 40
KroNI GCCGGC 1 cut(s) 42
KspAI GTTAAC 1 cut(s) 450
Kzo9I GATC 4 cut(s) 71, 85, 214, 598
LmnI GCTCC 1 cut(s) 433
Lsp1109I GCAGC 3 cut(s) 168, 322, 635
LweI GCATC 3 cut(s) 316, 463, 635
MaeI CTAG 2 cut(s) 570, 576
MaeII ACGT 2 cut(s) 352, 588
MaeIII GTNAC 1 cut(s) 319
MalI GATC 4 cut(s) 73, 87, 216, 600
MboI GATC 4 cut(s) 71, 85, 214, 598
MboII GAAGA 7 cut(s) 59, 183, 186, 229, 338, 378, 391
MflI RGATCY 2 cut(s) 214, 598
MluCI AATT 1 cut(s) 358
Mly113I GGCGCC 1 cut(s) 6
MmeI TCCRAC 2 cut(s) 45, 609
MroNI GCCGGC 1 cut(s) 40
MseI TTAA 3 cut(s) 357, 438, 449
MslI CAYNNNNRTG 2 cut(s) 59, 173
MspI CCGG 5 cut(s) 41, 146, 484, 517, 552
MspR9I CCNGG 1 cut(s) 146
MwoI GCNNNNNNNGC 3 cut(s) 139, 335, 552
NaeI GCCGGC 1 cut(s) 42
NarI GGCGCC 1 cut(s) 6
NciI CCSGG 1 cut(s) 146
NdeII GATC 4 cut(s) 71, 85, 214, 598
NgoMIV GCCGGC 1 cut(s) 40
NlaIV GGNNCC 3 cut(s) 7, 216, 505
OliI CACNNNNGTG 1 cut(s) 59
PaqCI CACCTGC 1 cut(s) 409
PdiI GCCGGC 1 cut(s) 42
PfeI GAWTC 2 cut(s) 14, 509
PkrI GCNGC 3 cut(s) 158, 337, 625
PluTI GGCGCC 1 cut(s) 9
PpuMI RGGWCCY 1 cut(s) 503
PshAI GACNNNNGTC 1 cut(s) 609
Psp1406I AACGTT 1 cut(s) 352
Psp5II RGGWCCY 1 cut(s) 503
PspN4I GGNNCC 3 cut(s) 7, 216, 505
PspPI GGNCC 1 cut(s) 503
PspPPI RGGWCCY 1 cut(s) 503
PsuI RGATCY 2 cut(s) 214, 598
RigI GGCCGGCC 1 cut(s) 44
RsaI GTAC 1 cut(s) 248
RsaNI GTAC 1 cut(s) 247
RseI CAYNNNNRTG 2 cut(s) 59, 173
SaqAI TTAA 3 cut(s) 357, 438, 449
SatI GCNGC 3 cut(s) 157, 336, 624
Sau3AI GATC 4 cut(s) 71, 85, 214, 598
Sau96I GGNCC 1 cut(s) 503
ScrFI CCNGG 1 cut(s) 146
SfaNI GCATC 3 cut(s) 316, 463, 635
SfoI GGCGCC 1 cut(s) 7
SinI GGWCC 1 cut(s) 503
SmiMI CAYNNNNRTG 2 cut(s) 59, 173
SmlI CTYRAG 1 cut(s) 25
SmoI CTYRAG 1 cut(s) 25
Sse9I AATT 1 cut(s) 358
SsiI CCGC 2 cut(s) 81, 621
SspDI GGCGCC 1 cut(s) 5
SspMI CTAG 2 cut(s) 570, 576
StyD4I CCNGG 1 cut(s) 144
TaaI ACNGT 4 cut(s) 61, 251, 470, 480
TaiI ACGT 2 cut(s) 355, 591
TaqI TCGA 1 cut(s) 122
TasI AATT 1 cut(s) 358
TfiI GAWTC 2 cut(s) 14, 509
Tru1I TTAA 3 cut(s) 357, 438, 449
Tru9I TTAA 3 cut(s) 357, 438, 449
TscAI CASTG 3 cut(s) 204, 372, 483
TseI GCWGC 3 cut(s) 156, 335, 623
TspDTI ATGAA 2 cut(s) 392, 398
TspGWI ACGGA 1 cut(s) 338
TspRI CASTG 3 cut(s) 204, 372, 483
VpaK11BI GGWCC 1 cut(s) 503
XmiI GTMKAC 1 cut(s) 613
XspI CTAG 2 cut(s) 570, 576
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.