RchiOBHm_Chr5g0080231

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0000811
Physical Location & Seq
Reverse (-)
86054610 .. 86059476
4867 bp
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UTR
Exon/CDS
Intron
PRQ35456

Sequence Viewer

Length: 486 bp
ATGCAGTATGAACCCCCAGTGTTGGAAAGAACATCGACGCCATTACTGACTTCACCCCCAAGTGAGGGTAGCTTGACTGACATTTTAGTGAGGAAACCTTCGTCTTCTTCAGCTCCCAGTATTGTGGATCCGAAAGTGTTACTGGAACTCTTCTCGATGTACCGTGAGTGGCAGGAGGAGAATGTCAAAAAGCTGAGCCAAAAACAGGAAGAGATAGAGAACAAGATAGAAATTGCGGACACGTTGTCTGTTAAACTTAAGCAACGTTTTAATTATTCAGTGACAGCAATGAAGACATCCTCGCAACATTTATGTGGAGTTCATGCACTGCAGGTAGAGATTGGGGAACTTAAAGGAAGGTTGACGGAGGTTATTAGCAACTGTGATGCAGTGTGCAAGAGAATATCCGCAGAAGGACCAGAGTCTCTTCGGTCATCTGTCAAGCCATTAGTTGTCTGCACCGCACACCAAGAACACCAATCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

161

Amino Acids

17.95

Weight (kDa)

6.1

Isoelectric Point (pI)

58.85

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 322
AciI CCGC 3 cut(s) 236, 408, 462
AclI AACGTT 1 cut(s) 265
AclWI GGATC 2 cut(s) 122, 135
AcuI CTGAAG 1 cut(s) 93
AcyI GRCGYC 1 cut(s) 38
AfaI GTAC 1 cut(s) 161
AfiI CCNNNNNNNGG 4 cut(s) 22, 64, 65, 205
AflII CTTAAG 1 cut(s) 257
AflIII ACRYGT 1 cut(s) 240
AhdI GACNNNNNGTC 1 cut(s) 244
AluBI AGCT 3 cut(s) 72, 113, 193
AluI AGCT 3 cut(s) 72, 113, 193
Alw26I GTCTC 1 cut(s) 429
AlwI GGATC 2 cut(s) 122, 135
AspS9I GGNCC 1 cut(s) 416
AsuHPI GGTGA 1 cut(s) 45
AvaII GGWCC 1 cut(s) 416
BamHI GGATCC 1 cut(s) 127
BbsI GAAGAC 2 cut(s) 96, 299
BcoDI GTCTC 1 cut(s) 429
BfmI CTRYAG 1 cut(s) 329
BfrI CTTAAG 1 cut(s) 257
BfuAI ACCTGC 1 cut(s) 322
BlpI GCTNAGC 1 cut(s) 194
Bme18I GGWCC 1 cut(s) 416
BmeRI GACNNNNNGTC 1 cut(s) 244
BmgT120I GGNCC 1 cut(s) 416
BmiI GGNNCC 1 cut(s) 129
BmrI ACTGGG 2 cut(s) 11, 111
BmsI GCATC 1 cut(s) 376
BmuI ACTGGG 2 cut(s) 11, 111
BoxI GACNNNNGTC 1 cut(s) 421
BpiI GAAGAC 2 cut(s) 96, 299
Bpu1102I GCTNAGC 1 cut(s) 194
BsaHI GRCGYC 1 cut(s) 38
Bsc4I CCNNNNNNNGG 4 cut(s) 22, 64, 65, 205
Bse1I ACTGG 3 cut(s) 17, 117, 147
Bse3DI GCAATG 1 cut(s) 294
BseGI GGATG 1 cut(s) 296
BseLI CCNNNNNNNGG 4 cut(s) 22, 64, 65, 205
BseMI GCAATG 1 cut(s) 294
BseMII CTCAG 1 cut(s) 185
BseNI ACTGG 3 cut(s) 17, 117, 147
BseRI GAGGAG 1 cut(s) 191
BsgI GTGCAG 1 cut(s) 442
BslI CCNNNNNNNGG 4 cut(s) 22, 64, 65, 205
BsmAI GTCTC 1 cut(s) 429
Bsp143I GATC 1 cut(s) 127
Bsp1720I GCTNAGC 1 cut(s) 194
BspACI CCGC 3 cut(s) 236, 408, 462
BspCNI CTCAG 1 cut(s) 186
BspLI GGNNCC 1 cut(s) 129
BspMAI CTGCAG 1 cut(s) 333
BspMI ACCTGC 1 cut(s) 322
BspPI GGATC 2 cut(s) 122, 135
BspTI CTTAAG 1 cut(s) 257
BsrDI GCAATG 1 cut(s) 294
BsrI ACTGG 3 cut(s) 17, 117, 147
BssMI GATC 1 cut(s) 127
BssNI GRCGYC 1 cut(s) 38
Bst4CI ACNGT 2 cut(s) 164, 383
Bst6I CTCTTC 3 cut(s) 155, 204, 432
BstACI GRCGYC 1 cut(s) 38
BstAFI CTTAAG 1 cut(s) 257
BstDEI CTNAG 1 cut(s) 194
BstF5I GGATG 1 cut(s) 296
BstKTI GATC 1 cut(s) 130
BstMAI GTCTC 1 cut(s) 429
BstMBI GATC 1 cut(s) 127
BstPAI GACNNNNGTC 1 cut(s) 421
BstSFI CTRYAG 1 cut(s) 329
BstV2I GAAGAC 2 cut(s) 96, 299
BstX2I RGATCY 1 cut(s) 127
BstXI CCANNNNNNTGG 1 cut(s) 124
BstYI RGATCY 1 cut(s) 127
BtsCI GGATG 1 cut(s) 296
BtsI GCAGTG 2 cut(s) 326, 396
BtsIMutI CAGTG 4 cut(s) 24, 285, 326, 396
BveI ACCTGC 1 cut(s) 322
Cfr13I GGNCC 1 cut(s) 416
CseI GACGC 1 cut(s) 46
Csp6I GTAC 1 cut(s) 160
CviAII CATG 1 cut(s) 323
CviJI RGCY 5 cut(s) 72, 113, 193, 198, 445
CviKI_1 RGCY 5 cut(s) 72, 113, 193, 198, 445
CviQI GTAC 1 cut(s) 160
DdeI CTNAG 1 cut(s) 194
DpnI GATC 1 cut(s) 129
DpnII GATC 1 cut(s) 127
DriI GACNNNNNGTC 1 cut(s) 244
Eam1104I CTCTTC 3 cut(s) 155, 204, 432
Eam1105I GACNNNNNGTC 1 cut(s) 244
EarI CTCTTC 3 cut(s) 155, 204, 432
Eco47I GGWCC 1 cut(s) 416
Eco57I CTGAAG 1 cut(s) 93
FaeI CATG 1 cut(s) 326
FaiI YATR 3 cut(s) 9, 313, 324
FatI CATG 1 cut(s) 322
FokI GGATG 1 cut(s) 283
HgaI GACGC 1 cut(s) 46
Hin1I GRCGYC 1 cut(s) 38
Hin1II CATG 1 cut(s) 326
HincII GTYRAC 1 cut(s) 363
HindII GTYRAC 1 cut(s) 363
HinfI GANTC 1 cut(s) 422
HphI GGTGA 1 cut(s) 45
Hpy166II GTNNAC 1 cut(s) 363
Hpy188I TCNGA 1 cut(s) 132
Hpy188III TCNNGA 2 cut(s) 154, 483
Hpy8I GTNNAC 1 cut(s) 363
Hpy99I CGWCG 1 cut(s) 40
HpyAV CCTTC 3 cut(s) 108, 351, 407
HpyCH4III ACNGT 2 cut(s) 164, 383
HpyCH4IV ACGT 2 cut(s) 242, 265
HpyCH4V TGCA 6 cut(s) 4, 326, 331, 389, 396, 459
HpyF3I CTNAG 1 cut(s) 194
HpySE526I ACGT 2 cut(s) 242, 265
Hsp92I GRCGYC 1 cut(s) 38
Hsp92II CATG 1 cut(s) 326
Kzo9I GATC 1 cut(s) 127
LmnI GCTCC 1 cut(s) 118
LpnPI CCDG 7 cut(s) 30, 128, 130, 158, 191, 317, 432
LweI GCATC 1 cut(s) 376
MaeII ACGT 2 cut(s) 242, 265
MaeIII GTNAC 2 cut(s) 138, 280
MalI GATC 1 cut(s) 129
MboI GATC 1 cut(s) 127
MboII GAAGA 6 cut(s) 96, 99, 142, 221, 304, 419
MflI RGATCY 1 cut(s) 127
MluCI AATT 2 cut(s) 231, 271
MlyI GAGTC 1 cut(s) 431
MnlI CCTC 5 cut(s) 58, 84, 169, 310, 361
MseI TTAA 4 cut(s) 252, 258, 270, 351
MslI CAYNNNNRTG 2 cut(s) 86, 312
MspCI CTTAAG 1 cut(s) 257
NdeII GATC 1 cut(s) 127
NlaIII CATG 1 cut(s) 326
NlaIV GGNNCC 1 cut(s) 129
NmuCI GTSAC 1 cut(s) 280
PleI GAGTC 1 cut(s) 430
PpsI GAGTC 1 cut(s) 430
PshAI GACNNNNGTC 1 cut(s) 421
Psp1406I AACGTT 1 cut(s) 265
PspN4I GGNNCC 1 cut(s) 129
PspPI GGNCC 1 cut(s) 416
PstI CTGCAG 1 cut(s) 333
PsuI RGATCY 1 cut(s) 127
RsaI GTAC 1 cut(s) 161
RsaNI GTAC 1 cut(s) 160
RseI CAYNNNNRTG 2 cut(s) 86, 312
SaqAI TTAA 4 cut(s) 252, 258, 270, 351
Sau3AI GATC 1 cut(s) 127
Sau96I GGNCC 1 cut(s) 416
SchI GAGTC 1 cut(s) 431
SetI ASST 9 cut(s) 74, 100, 115, 195, 245, 268, 336, 362, 372
SfaNI GCATC 1 cut(s) 376
SfcI CTRYAG 1 cut(s) 329
SinI GGWCC 1 cut(s) 416
SmiMI CAYNNNNRTG 2 cut(s) 86, 312
SmlI CTYRAG 1 cut(s) 257
SmoI CTYRAG 1 cut(s) 257
Sse9I AATT 2 cut(s) 231, 271
SsiI CCGC 3 cut(s) 236, 408, 462
TaaI ACNGT 2 cut(s) 164, 383
TaiI ACGT 2 cut(s) 245, 268
TaqI TCGA 2 cut(s) 35, 155
TaqII GACCGA 1 cut(s) 420
TasI AATT 2 cut(s) 231, 271
Tru1I TTAA 4 cut(s) 252, 258, 270, 351
Tru9I TTAA 4 cut(s) 252, 258, 270, 351
TscAI CASTG 4 cut(s) 24, 285, 333, 396
TseFI GTSAC 1 cut(s) 280
Tsp45I GTSAC 1 cut(s) 280
TspDTI ATGAA 3 cut(s) 24, 305, 311
TspGWI ACGGA 1 cut(s) 380
TspRI CASTG 4 cut(s) 24, 285, 333, 396
Vha464I CTTAAG 1 cut(s) 257
VpaK11BI GGWCC 1 cut(s) 416
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.