Rmu_ssc0000366.1_g000005

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000366.1
OG0000811
Physical Location & Seq
Forward (+)
27196 .. 34564
7369 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_ssc0000366.1_g000005.1.cds

Sequence Viewer

Length: 1179 bp
atgtgcaaagaacgaagggtagaaagcttgcgtcgtcaccatcaggaacgcaacttgcgacttcaacgacatgacttgacatggtctgaacgacgggtgatctggaaaagtgttgttcatggttatttaaccatcagcttctctaattttggcgatgcattcgagatcaacgatgttctatgtgtggaagcatgtgagccttcagtcttcaagagagaactgatccagaaaactcgagatagagaaccagagttctctgacccgtcgcgacccaaaactcgcaaggaggacccggctgtcgcgccaccttccggccaccccacgacggcgcgcgggcaccaatggcttcctctcctcgtcgccgacgtatctctggggtcaggtctctcaaccggcagctctggagcgagaatcgacaccatgaagtctcatgttttccgatctcatctcaagtccgccatgatcctcataccagagagaccgctagtgaagcgaccagattctgatcaggtccctatcatggagcaactggagcatactagacagggaggctatgagaagaggaacttccgtcttcttgcccactgctacttatcccatgttcgcagaaaaacttgccatggagggaatgagtttgcagcaaggagagatttgcagtaccgttataggtctatggaccctcacttcccgtctagatttgtgaatatgggaagagttatgttggggttggagatggattggatcagatatgttgtgaagttggaaggaatccatatttctctacagactgaaaagagtaacagatcagagatggtgggtctatggaccctggctacctgccgagatttgtcaatatgggtttacctatttggagaatgtggggatgttaaagacgttgaggaagagatagagaacaagatagaaattgcggacgcgttgtctgttaaacttaagcaacgttttaattattcagtgacagcaatgaagacatcctcgcaacatttatgtggagttcatgcactgcaggtagagattggggaacttaaaggaaggttgacggaggttattagcaactgtgatgcagtgtgcaagagaatatccgcagaaggaccagagtctcttcggtcatctgtcaagccgttagttatctgcaccgcacaccaagaacaccaatcttga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

392

Amino Acids

44.95

Weight (kDa)

8.9

Isoelectric Point (pI)

52.95

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 296
Acc36I ACCTGC 2 cut(s) 845, 1015
AccB1I GGYRCC 1 cut(s) 336
AccII CGCG 5 cut(s) 268, 302, 331, 333, 935
AciI CCGC 6 cut(s) 333, 456, 482, 929, 1101, 1155
AclI AACGTT 1 cut(s) 958
AclWI GGATC 3 cut(s) 217, 457, 749
AcoI YGGCCR 1 cut(s) 313
AcuI CTGAAG 1 cut(s) 186
AfaI GTAC 1 cut(s) 659
AfiI CCNNNNNNNGG 3 cut(s) 311, 325, 520
AflII CTTAAG 1 cut(s) 950
AflIII ACRYGT 1 cut(s) 933
AgsI TTSAA 2 cut(s) 65, 211
AhdI GACNNNNNGTC 1 cut(s) 937
AjnI CCWGG 1 cut(s) 828
AluBI AGCT 3 cut(s) 27, 138, 399
AluI AGCT 3 cut(s) 27, 138, 399
Alw26I GTCTC 4 cut(s) 389, 432, 472, 1122
AlwI GGATC 3 cut(s) 217, 457, 749
AlwNI CAGNNNCTG 1 cut(s) 503
Ama87I CYCGRG 1 cut(s) 234
AoxI GGCC 1 cut(s) 313
ApeKI GCWGC 2 cut(s) 396, 638
AspLEI GCGC 3 cut(s) 304, 331, 333
AspS9I GGNCC 5 cut(s) 289, 511, 676, 825, 1109
AsuC2I CCSGG 1 cut(s) 293
AsuHPI GGTGA 2 cut(s) 29, 109
AvaI CYCGRG 1 cut(s) 234
AvaII GGWCC 5 cut(s) 289, 511, 676, 825, 1109
BaeGI GKGCMC 1 cut(s) 339
BanI GGYRCC 1 cut(s) 336
BbsI GAAGAC 3 cut(s) 199, 566, 992
BbvI GCAGC 2 cut(s) 408, 650
BccI CCATC 4 cut(s) 48, 140, 727, 805
BceAI ACGGC 2 cut(s) 342, 1123
BciT130I CCWGG 1 cut(s) 830
BclI TGATCA 1 cut(s) 505
BcnI CCSGG 1 cut(s) 293
BcoDI GTCTC 4 cut(s) 389, 432, 472, 1122
BfaI CTAG 3 cut(s) 485, 540, 693
BfmI CTRYAG 2 cut(s) 782, 1022
BfrI CTTAAG 1 cut(s) 950
BfuAI ACCTGC 2 cut(s) 845, 1015
BisI GCNGC 2 cut(s) 397, 639
BlsI GCNGC 2 cut(s) 398, 640
Bme1390I CCNGG 2 cut(s) 293, 830
Bme18I GGWCC 5 cut(s) 289, 511, 676, 825, 1109
BmeRI GACNNNNNGTC 1 cut(s) 937
BmeT110I CYCGRG 1 cut(s) 234
BmgT120I GGNCC 5 cut(s) 289, 511, 676, 825, 1109
BmiI GGNNCC 5 cut(s) 291, 338, 513, 678, 827
BmrFI CCNGG 2 cut(s) 293, 830
BmsI GCATC 2 cut(s) 145, 1069
BoxI GACNNNNGTC 1 cut(s) 1114
BpiI GAAGAC 3 cut(s) 199, 566, 992
BpmI CTGGAG 2 cut(s) 423, 551
BpuEI CTTGAG 1 cut(s) 434
BpuMI CCSGG 1 cut(s) 293
BsaBI GATNNNNATC 1 cut(s) 504
BsaI GGTCTC 2 cut(s) 389, 472
BsaJI CCNNGG 2 cut(s) 619, 828
Bsc4I CCNNNNNNNGG 3 cut(s) 311, 325, 520
Bse118I RCCGGY 1 cut(s) 392
Bse1I ACTGG 1 cut(s) 534
Bse3DI GCAATG 1 cut(s) 987
Bse8I GATNNNNATC 1 cut(s) 504
BseBI CCWGG 1 cut(s) 830
BseDI CCNNGG 2 cut(s) 619, 828
BseGI GGATG 2 cut(s) 889, 989
BseJI GATNNNNATC 1 cut(s) 504
BseLI CCNNNNNNNGG 3 cut(s) 311, 325, 520
BseMI GCAATG 1 cut(s) 987
BseNI ACTGG 1 cut(s) 534
BsePI GCGCGC 1 cut(s) 329
BseRI GAGGAG 1 cut(s) 344
BseSI GKGCMC 1 cut(s) 339
BseXI GCAGC 2 cut(s) 408, 650
BsgI GTGCAG 1 cut(s) 1135
Bsh1236I CGCG 5 cut(s) 268, 302, 331, 333, 935
BshFI GGCC 1 cut(s) 315
BshNI GGYRCC 1 cut(s) 336
BsiHKCI CYCGRG 1 cut(s) 234
BsiSI CCGG 3 cut(s) 293, 312, 393
BslFI GGGAC 1 cut(s) 497
BslI CCNNNNNNNGG 3 cut(s) 311, 325, 520
BsmAI GTCTC 4 cut(s) 389, 432, 472, 1122
BsmFI GGGAC 1 cut(s) 497
BsmI GAATGC 1 cut(s) 158
BsnI GGCC 1 cut(s) 315
Bso31I GGTCTC 2 cut(s) 389, 472
BsoBI CYCGRG 1 cut(s) 234
Bsp1286I GDGCHC 1 cut(s) 339
Bsp143I GATC 8 cut(s) 99, 165, 222, 440, 462, 505, 741, 803
Bsp19I CCATGG 1 cut(s) 619
Bsp68I TCGCGA 1 cut(s) 268
BspACI CCGC 6 cut(s) 333, 456, 482, 929, 1101, 1155
BspANI GGCC 1 cut(s) 315
BspFNI CGCG 5 cut(s) 268, 302, 331, 333, 935
BspLI GGNNCC 5 cut(s) 291, 338, 513, 678, 827
BspMAI CTGCAG 1 cut(s) 1026
BspMI ACCTGC 2 cut(s) 845, 1015
BspPI GGATC 3 cut(s) 217, 457, 749
BspT107I GGYRCC 1 cut(s) 336
BspTI CTTAAG 1 cut(s) 950
BspTNI GGTCTC 2 cut(s) 389, 472
BsrDI GCAATG 1 cut(s) 987
BsrFI RCCGGY 1 cut(s) 392
BsrI ACTGG 1 cut(s) 534
BssAI RCCGGY 1 cut(s) 392
BssECI CCNNGG 2 cut(s) 619, 828
BssHII GCGCGC 1 cut(s) 329
BssMI GATC 8 cut(s) 99, 165, 222, 440, 462, 505, 741, 803
BssT1I CCWWGG 1 cut(s) 619
Bst2UI CCWGG 1 cut(s) 830
Bst4CI ACNGT 2 cut(s) 662, 1076
Bst6I CTCTTC 4 cut(s) 554, 706, 897, 1125
BstAFI CTTAAG 1 cut(s) 950
BstC8I GCNNGC 3 cut(s) 29, 331, 335
BstDSI CCRYGG 1 cut(s) 619
BstF5I GGATG 2 cut(s) 889, 989
BstFNI CGCG 5 cut(s) 268, 302, 331, 333, 935
BstHHI GCGC 3 cut(s) 304, 331, 333
BstKTI GATC 8 cut(s) 102, 168, 225, 443, 465, 508, 744, 806
BstMAI GTCTC 4 cut(s) 389, 432, 472, 1122
BstMBI GATC 8 cut(s) 99, 165, 222, 440, 462, 505, 741, 803
BstMWI GCNNNNNNNGC 3 cut(s) 343, 490, 532
BstNI CCWGG 1 cut(s) 830
BstNSI RCATGY 1 cut(s) 195
BstPAI GACNNNNGTC 1 cut(s) 1114
BstSCI CCNGG 2 cut(s) 291, 828
BstSFI CTRYAG 2 cut(s) 782, 1022
BstSLI GKGCMC 1 cut(s) 339
BstUI CGCG 5 cut(s) 268, 302, 331, 333, 935
BstV1I GCAGC 2 cut(s) 408, 650
BstV2I GAAGAC 3 cut(s) 199, 566, 992
BsuRI GGCC 1 cut(s) 315
BtgI CCRYGG 1 cut(s) 619
BtgZI GCGATG 1 cut(s) 168
BtsCI GGATG 2 cut(s) 889, 989
BtsI GCAGTG 3 cut(s) 583, 1019, 1089
BtsIMutI CAGTG 4 cut(s) 583, 978, 1019, 1089
BtuMI TCGCGA 1 cut(s) 268
BveI ACCTGC 2 cut(s) 845, 1015
Cac8I GCNNGC 3 cut(s) 29, 331, 335
CaiI CAGNNNCTG 1 cut(s) 503
CfoI GCGC 3 cut(s) 304, 331, 333
Cfr10I RCCGGY 1 cut(s) 392
Cfr13I GGNCC 5 cut(s) 289, 511, 676, 825, 1109
CseI GACGC 2 cut(s) 20, 941
Csp6I GTAC 1 cut(s) 658
CviQI GTAC 1 cut(s) 658
DpnI GATC 8 cut(s) 101, 167, 224, 442, 464, 507, 743, 805
DpnII GATC 8 cut(s) 99, 165, 222, 440, 462, 505, 741, 803
DrdI GACNNNNNNGTC 1 cut(s) 296
DriI GACNNNNNGTC 1 cut(s) 937
DseDI GACNNNNNNGTC 1 cut(s) 296
EaeI YGGCCR 1 cut(s) 313
Eam1104I CTCTTC 4 cut(s) 554, 706, 897, 1125
Eam1105I GACNNNNNGTC 1 cut(s) 937
EarI CTCTTC 4 cut(s) 554, 706, 897, 1125
EciI GGCGGA 1 cut(s) 445
Eco130I CCWWGG 1 cut(s) 619
Eco31I GGTCTC 2 cut(s) 389, 472
Eco47I GGWCC 5 cut(s) 289, 511, 676, 825, 1109
Eco57I CTGAAG 1 cut(s) 186
Eco88I CYCGRG 1 cut(s) 234
EcoO109I RGGNCCY 2 cut(s) 289, 511
EcoRII CCWGG 1 cut(s) 828
EcoT14I CCWWGG 1 cut(s) 619
EcoT22I ATGCAT 1 cut(s) 160
ErhI CCWWGG 1 cut(s) 619
FalI AAGNNNNNCTT 2 cut(s) 551, 583
FaqI GGGAC 1 cut(s) 497
FauI CCCGC 1 cut(s) 326
FbaI TGATCA 1 cut(s) 505
Fnu4HI GCNGC 2 cut(s) 397, 639
FokI GGATG 2 cut(s) 896, 976
Fsp4HI GCNGC 2 cut(s) 397, 639
FspBI CTAG 3 cut(s) 485, 540, 693
GlaI GCGC 3 cut(s) 303, 330, 332
GluI GCNGC 2 cut(s) 397, 639
GsuI CTGGAG 2 cut(s) 423, 551
HaeIII GGCC 1 cut(s) 315
HapII CCGG 3 cut(s) 293, 312, 393
HgaI GACGC 2 cut(s) 20, 941
HhaI GCGC 3 cut(s) 304, 331, 333
Hin6I GCGC 3 cut(s) 302, 329, 331
HinP1I GCGC 3 cut(s) 302, 329, 331
HincII GTYRAC 1 cut(s) 1056
HindII GTYRAC 1 cut(s) 1056
HindIII AAGCTT 1 cut(s) 25
HinfI GANTC 4 cut(s) 411, 500, 768, 1115
HpaII CCGG 3 cut(s) 293, 312, 393
HphI GGTGA 2 cut(s) 29, 109
Hpy166II GTNNAC 2 cut(s) 862, 1056
Hpy188I TCNGA 6 cut(s) 88, 259, 440, 505, 746, 808
Hpy8I GTNNAC 2 cut(s) 862, 1056
Hpy99I CGWCG 6 cut(s) 36, 96, 268, 328, 362, 368
HpyAV CCTTC 6 cut(s) 9, 210, 318, 758, 1044, 1100
HpyCH4III ACNGT 2 cut(s) 662, 1076
HpyCH4IV ACGT 3 cut(s) 366, 894, 958
HpyCH4V TGCA 9 cut(s) 6, 158, 638, 655, 1019, 1024, 1082, 1089, 1152
HpyF10VI GCNNNNNNNGC 3 cut(s) 343, 490, 532
HpySE526I ACGT 3 cut(s) 366, 894, 958
HspAI GCGC 3 cut(s) 302, 329, 331
Ksp22I TGATCA 1 cut(s) 505
Kzo9I GATC 8 cut(s) 99, 165, 222, 440, 462, 505, 741, 803
LmnI GCTCC 3 cut(s) 404, 523, 532
Lsp1109I GCAGC 2 cut(s) 408, 650
LweI GCATC 2 cut(s) 145, 1069
MaeI CTAG 3 cut(s) 485, 540, 693
MaeII ACGT 3 cut(s) 366, 894, 958
MaeIII GTNAC 3 cut(s) 35, 797, 973
MalI GATC 8 cut(s) 101, 167, 224, 442, 464, 507, 743, 805
MboI GATC 8 cut(s) 99, 165, 222, 440, 462, 505, 741, 803
MboII GAAGA 7 cut(s) 199, 566, 571, 723, 914, 997, 1112
MhlI GDGCHC 1 cut(s) 339
MluCI AATT 3 cut(s) 145, 924, 964
MluI ACGCGT 1 cut(s) 933
MlyI GAGTC 1 cut(s) 1124
MmeI TCCRAC 2 cut(s) 708, 741
Mph1103I ATGCAT 1 cut(s) 160
MseI TTAA 6 cut(s) 128, 888, 945, 951, 963, 1044
MslI CAYNNNNRTG 1 cut(s) 1005
MspCI CTTAAG 1 cut(s) 950
MspI CCGG 3 cut(s) 293, 312, 393
MspR9I CCNGG 2 cut(s) 293, 830
Mva1269I GAATGC 1 cut(s) 158
MvaI CCWGG 1 cut(s) 830
MvnI CGCG 5 cut(s) 268, 302, 331, 333, 935
MwoI GCNNNNNNNGC 3 cut(s) 343, 490, 532
NciI CCSGG 1 cut(s) 293
NcoI CCATGG 1 cut(s) 619
NdeII GATC 8 cut(s) 99, 165, 222, 440, 462, 505, 741, 803
NlaIV GGNNCC 5 cut(s) 291, 338, 513, 678, 827
NmeAIII GCCGAG 1 cut(s) 866
NmuCI GTSAC 2 cut(s) 35, 973
NruI TCGCGA 1 cut(s) 268
NsiI ATGCAT 1 cut(s) 160
NspI RCATGY 1 cut(s) 195
PaeR7I CTCGAG 1 cut(s) 234
PauI GCGCGC 1 cut(s) 329
PcsI WCGNNNNNNNCGW 3 cut(s) 55, 168, 363
PctI GAATGC 1 cut(s) 158
PfeI GAWTC 3 cut(s) 411, 500, 768
PflFI GACNNNGTC 1 cut(s) 82
PkrI GCNGC 2 cut(s) 398, 640
PleI GAGTC 1 cut(s) 1123
PpsI GAGTC 1 cut(s) 1123
PpuMI RGGWCCY 2 cut(s) 289, 511
PshAI GACNNNNGTC 1 cut(s) 1114
Psp1406I AACGTT 1 cut(s) 958
Psp5II RGGWCCY 2 cut(s) 289, 511
Psp6I CCWGG 1 cut(s) 828
PspGI CCWGG 1 cut(s) 828
PspN4I GGNNCC 5 cut(s) 291, 338, 513, 678, 827
PspPI GGNCC 5 cut(s) 289, 511, 676, 825, 1109
PspPPI RGGWCCY 2 cut(s) 289, 511
PstI CTGCAG 1 cut(s) 1026
PstNI CAGNNNCTG 1 cut(s) 503
PsyI GACNNNGTC 1 cut(s) 82
PteI GCGCGC 1 cut(s) 329
RruI TCGCGA 1 cut(s) 268
RsaI GTAC 1 cut(s) 659
RsaNI GTAC 1 cut(s) 658
RseI CAYNNNNRTG 1 cut(s) 1005
SaqAI TTAA 6 cut(s) 128, 888, 945, 951, 963, 1044
SatI GCNGC 2 cut(s) 397, 639
Sau3AI GATC 8 cut(s) 99, 165, 222, 440, 462, 505, 741, 803
Sau96I GGNCC 5 cut(s) 289, 511, 676, 825, 1109
SchI GAGTC 1 cut(s) 1124
ScrFI CCNGG 2 cut(s) 293, 830
SduI GDGCHC 1 cut(s) 339
SfaNI GCATC 2 cut(s) 145, 1069
SfcI CTRYAG 2 cut(s) 782, 1022
Sfr274I CTCGAG 1 cut(s) 234
SinI GGWCC 5 cut(s) 289, 511, 676, 825, 1109
SlaI CTCGAG 1 cut(s) 234
SmiMI CAYNNNNRTG 1 cut(s) 1005
SmlI CTYRAG 3 cut(s) 234, 449, 950
SmoI CTYRAG 3 cut(s) 234, 449, 950
Sse9I AATT 3 cut(s) 145, 924, 964
SsiI CCGC 6 cut(s) 333, 456, 482, 929, 1101, 1155
SspMI CTAG 3 cut(s) 485, 540, 693
StyD4I CCNGG 2 cut(s) 291, 828
StyI CCWWGG 1 cut(s) 619
TaaI ACNGT 2 cut(s) 662, 1076
TaiI ACGT 3 cut(s) 369, 897, 961
TaqI TCGA 3 cut(s) 162, 235, 414
TaqII GACCGA 1 cut(s) 1113
TasI AATT 3 cut(s) 145, 924, 964
TfiI GAWTC 3 cut(s) 411, 500, 768
Tru1I TTAA 6 cut(s) 128, 888, 945, 951, 963, 1044
Tru9I TTAA 6 cut(s) 128, 888, 945, 951, 963, 1044
TscAI CASTG 4 cut(s) 590, 978, 1026, 1089
TseFI GTSAC 2 cut(s) 35, 973
TseI GCWGC 2 cut(s) 396, 638
Tsp45I GTSAC 2 cut(s) 35, 973
TspDTI ATGAA 4 cut(s) 107, 437, 998, 1004
TspGWI ACGGA 2 cut(s) 560, 1073
TspRI CASTG 4 cut(s) 590, 978, 1026, 1089
Tth111I GACNNNGTC 1 cut(s) 82
Vha464I CTTAAG 1 cut(s) 950
VpaK11BI GGWCC 5 cut(s) 289, 511, 676, 825, 1109
XbaI TCTAGA 1 cut(s) 692
XceI RCATGY 1 cut(s) 195
XhoI CTCGAG 1 cut(s) 234
XspI CTAG 3 cut(s) 485, 540, 693
Zsp2I ATGCAT 1 cut(s) 160
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.