Rw4G013560

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0000811
Physical Location & Seq
Reverse (-)
31677611 .. 31680832
3222 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G013560.1

Sequence Viewer

Length: 543 bp
ATGGGCTACGGTAATACCACTCTAGATCTGGAATGGGTTCCTGAACCTCTCATTCCTCCTATCACAAAAGATATGCAGTATGAACCCCCATCGACGCCATTACTGACTTCACCCCCAAGTGAGGGTAGCTTGACTGACATTTTAGTGAGGAAACCTTCGTCTTCTTCAGCTCCCGGTATTGTGGATCCGAAAGTGTTACTGGAACTCTTCTCGATGTACCGTGAGTGGCAGGAGGAGAATGTCAAAAAGCTGAGCCAAAAACAGGAAGAGATAGAGAACAAGATAGAAATTGCGGACGCGTTGTCTGTTAAACTTAAGCAACGTTTTAATTATTCAGTGACAGCAATGAAGACATCCTCGCAACATTTATGTGGAGTTCATGCACTGCAGGTAGAGATTGGGGAACTTAAAGGAAGGTTGACGGAGGTTATTAGCAACTGTGATGCAGTGTGCAAGAGAATATCCGCAGAAGGACCAGAGTCTCTTCGGTCATCTGTCAAGCCATTAGTTATCTGCACCGCACACCAAGAACACCAATCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

180

Amino Acids

19.99

Weight (kDa)

5.44

Isoelectric Point (pI)

53.81

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 379
AccII CGCG 1 cut(s) 299
AciI CCGC 3 cut(s) 293, 465, 519
AclI AACGTT 1 cut(s) 322
AclWI GGATC 2 cut(s) 179, 192
AcuI CTGAAG 1 cut(s) 150
AcyI GRCGYC 1 cut(s) 95
AfaI GTAC 1 cut(s) 218
AfiI CCNNNNNNNGG 3 cut(s) 121, 122, 262
AflII CTTAAG 1 cut(s) 314
AflIII ACRYGT 1 cut(s) 297
AhdI GACNNNNNGTC 1 cut(s) 301
AluBI AGCT 3 cut(s) 129, 170, 250
AluI AGCT 3 cut(s) 129, 170, 250
Alw26I GTCTC 1 cut(s) 486
AlwI GGATC 2 cut(s) 179, 192
Asp700I GAANNNNTTC 1 cut(s) 36
AspS9I GGNCC 1 cut(s) 473
AsuC2I CCSGG 1 cut(s) 174
AsuHPI GGTGA 1 cut(s) 102
AvaII GGWCC 1 cut(s) 473
BamHI GGATCC 1 cut(s) 184
BbsI GAAGAC 2 cut(s) 153, 356
BccI CCATC 1 cut(s) 97
BcnI CCSGG 1 cut(s) 174
BcoDI GTCTC 1 cut(s) 486
BfaI CTAG 1 cut(s) 23
BfmI CTRYAG 1 cut(s) 386
BfrI CTTAAG 1 cut(s) 314
BfuAI ACCTGC 1 cut(s) 379
BglII AGATCT 1 cut(s) 25
BlpI GCTNAGC 1 cut(s) 251
Bme1390I CCNGG 1 cut(s) 174
Bme18I GGWCC 1 cut(s) 473
BmeRI GACNNNNNGTC 1 cut(s) 301
BmgT120I GGNCC 1 cut(s) 473
BmiI GGNNCC 2 cut(s) 39, 186
BmrFI CCNGG 1 cut(s) 174
BmsI GCATC 1 cut(s) 433
BoxI GACNNNNGTC 1 cut(s) 478
BpiI GAAGAC 2 cut(s) 153, 356
Bpu1102I GCTNAGC 1 cut(s) 251
BpuMI CCSGG 1 cut(s) 174
BsaHI GRCGYC 1 cut(s) 95
Bsc4I CCNNNNNNNGG 3 cut(s) 121, 122, 262
Bse1I ACTGG 1 cut(s) 204
Bse3DI GCAATG 1 cut(s) 351
BseGI GGATG 1 cut(s) 353
BseLI CCNNNNNNNGG 3 cut(s) 121, 122, 262
BseMI GCAATG 1 cut(s) 351
BseMII CTCAG 1 cut(s) 242
BseNI ACTGG 1 cut(s) 204
BseRI GAGGAG 1 cut(s) 248
BsgI GTGCAG 1 cut(s) 499
Bsh1236I CGCG 1 cut(s) 299
BsiSI CCGG 1 cut(s) 174
BslI CCNNNNNNNGG 3 cut(s) 121, 122, 262
BsmAI GTCTC 1 cut(s) 486
Bsp143I GATC 2 cut(s) 25, 184
Bsp1720I GCTNAGC 1 cut(s) 251
BspACI CCGC 3 cut(s) 293, 465, 519
BspCNI CTCAG 1 cut(s) 243
BspFNI CGCG 1 cut(s) 299
BspLI GGNNCC 2 cut(s) 39, 186
BspMAI CTGCAG 1 cut(s) 390
BspMI ACCTGC 1 cut(s) 379
BspPI GGATC 2 cut(s) 179, 192
BspTI CTTAAG 1 cut(s) 314
BsrDI GCAATG 1 cut(s) 351
BsrI ACTGG 1 cut(s) 204
BssMI GATC 2 cut(s) 25, 184
BssNI GRCGYC 1 cut(s) 95
Bst4CI ACNGT 3 cut(s) 11, 221, 440
Bst6I CTCTTC 3 cut(s) 212, 261, 489
BstACI GRCGYC 1 cut(s) 95
BstAFI CTTAAG 1 cut(s) 314
BstDEI CTNAG 1 cut(s) 251
BstF5I GGATG 1 cut(s) 353
BstFNI CGCG 1 cut(s) 299
BstKTI GATC 2 cut(s) 28, 187
BstMAI GTCTC 1 cut(s) 486
BstMBI GATC 2 cut(s) 25, 184
BstPAI GACNNNNGTC 1 cut(s) 478
BstSCI CCNGG 1 cut(s) 172
BstSFI CTRYAG 1 cut(s) 386
BstUI CGCG 1 cut(s) 299
BstV2I GAAGAC 2 cut(s) 153, 356
BstX2I RGATCY 2 cut(s) 25, 184
BstYI RGATCY 2 cut(s) 25, 184
BtsCI GGATG 1 cut(s) 353
BtsI GCAGTG 2 cut(s) 383, 453
BtsIMutI CAGTG 3 cut(s) 342, 383, 453
BveI ACCTGC 1 cut(s) 379
Cfr13I GGNCC 1 cut(s) 473
CseI GACGC 2 cut(s) 103, 305
Csp6I GTAC 1 cut(s) 217
CviAII CATG 1 cut(s) 380
CviJI RGCY 6 cut(s) 6, 129, 170, 250, 255, 502
CviKI_1 RGCY 6 cut(s) 6, 129, 170, 250, 255, 502
CviQI GTAC 1 cut(s) 217
DdeI CTNAG 1 cut(s) 251
DpnI GATC 2 cut(s) 27, 186
DpnII GATC 2 cut(s) 25, 184
DriI GACNNNNNGTC 1 cut(s) 301
Eam1104I CTCTTC 3 cut(s) 212, 261, 489
Eam1105I GACNNNNNGTC 1 cut(s) 301
EarI CTCTTC 3 cut(s) 212, 261, 489
Eco47I GGWCC 1 cut(s) 473
Eco57I CTGAAG 1 cut(s) 150
FaeI CATG 1 cut(s) 383
FaiI YATR 4 cut(s) 74, 81, 370, 381
FatI CATG 1 cut(s) 379
FokI GGATG 1 cut(s) 340
FspBI CTAG 1 cut(s) 23
HapII CCGG 1 cut(s) 174
HgaI GACGC 2 cut(s) 103, 305
Hin1I GRCGYC 1 cut(s) 95
Hin1II CATG 1 cut(s) 383
HincII GTYRAC 1 cut(s) 420
HindII GTYRAC 1 cut(s) 420
HinfI GANTC 1 cut(s) 479
HpaII CCGG 1 cut(s) 174
HphI GGTGA 1 cut(s) 102
Hpy166II GTNNAC 1 cut(s) 420
Hpy188I TCNGA 1 cut(s) 189
Hpy188III TCNNGA 5 cut(s) 23, 29, 41, 211, 540
Hpy8I GTNNAC 1 cut(s) 420
Hpy99I CGWCG 1 cut(s) 97
HpyAV CCTTC 3 cut(s) 165, 408, 464
HpyCH4III ACNGT 3 cut(s) 11, 221, 440
HpyCH4IV ACGT 1 cut(s) 322
HpyCH4V TGCA 6 cut(s) 76, 383, 388, 446, 453, 516
HpyF3I CTNAG 1 cut(s) 251
HpySE526I ACGT 1 cut(s) 322
Hsp92I GRCGYC 1 cut(s) 95
Hsp92II CATG 1 cut(s) 383
Kzo9I GATC 2 cut(s) 25, 184
LmnI GCTCC 1 cut(s) 175
LpnPI CCDG 8 cut(s) 14, 54, 185, 187, 215, 248, 374, 489
LweI GCATC 1 cut(s) 433
MaeI CTAG 1 cut(s) 23
MaeII ACGT 1 cut(s) 322
MaeIII GTNAC 2 cut(s) 195, 337
MalI GATC 2 cut(s) 27, 186
MboI GATC 2 cut(s) 25, 184
MboII GAAGA 6 cut(s) 153, 156, 199, 278, 361, 476
MflI RGATCY 2 cut(s) 25, 184
MluCI AATT 2 cut(s) 288, 328
MluI ACGCGT 1 cut(s) 297
MlyI GAGTC 1 cut(s) 488
MnlI CCTC 7 cut(s) 57, 66, 115, 141, 226, 367, 418
MroXI GAANNNNTTC 1 cut(s) 36
MseI TTAA 4 cut(s) 309, 315, 327, 408
MslI CAYNNNNRTG 2 cut(s) 143, 369
MspCI CTTAAG 1 cut(s) 314
MspI CCGG 1 cut(s) 174
MspR9I CCNGG 1 cut(s) 174
MvnI CGCG 1 cut(s) 299
NciI CCSGG 1 cut(s) 174
NdeII GATC 2 cut(s) 25, 184
NlaIII CATG 1 cut(s) 383
NlaIV GGNNCC 2 cut(s) 39, 186
NmuCI GTSAC 1 cut(s) 337
PdmI GAANNNNTTC 1 cut(s) 36
PleI GAGTC 1 cut(s) 487
PpsI GAGTC 1 cut(s) 487
PshAI GACNNNNGTC 1 cut(s) 478
Psp1406I AACGTT 1 cut(s) 322
PspN4I GGNNCC 2 cut(s) 39, 186
PspPI GGNCC 1 cut(s) 473
PstI CTGCAG 1 cut(s) 390
PsuI RGATCY 2 cut(s) 25, 184
RsaI GTAC 1 cut(s) 218
RsaNI GTAC 1 cut(s) 217
RseI CAYNNNNRTG 2 cut(s) 143, 369
SaqAI TTAA 4 cut(s) 309, 315, 327, 408
Sau3AI GATC 2 cut(s) 25, 184
Sau96I GGNCC 1 cut(s) 473
SchI GAGTC 1 cut(s) 488
ScrFI CCNGG 1 cut(s) 174
SetI ASST 9 cut(s) 49, 131, 157, 172, 252, 325, 393, 419, 429
SfaNI GCATC 1 cut(s) 433
SfcI CTRYAG 1 cut(s) 386
SinI GGWCC 1 cut(s) 473
SmiMI CAYNNNNRTG 2 cut(s) 143, 369
SmlI CTYRAG 1 cut(s) 314
SmoI CTYRAG 1 cut(s) 314
Sse9I AATT 2 cut(s) 288, 328
SsiI CCGC 3 cut(s) 293, 465, 519
SspMI CTAG 1 cut(s) 23
StyD4I CCNGG 1 cut(s) 172
TaaI ACNGT 3 cut(s) 11, 221, 440
TaiI ACGT 1 cut(s) 325
TaqI TCGA 2 cut(s) 92, 212
TaqII GACCGA 1 cut(s) 477
TasI AATT 2 cut(s) 288, 328
Tru1I TTAA 4 cut(s) 309, 315, 327, 408
Tru9I TTAA 4 cut(s) 309, 315, 327, 408
TscAI CASTG 3 cut(s) 342, 390, 453
TseFI GTSAC 1 cut(s) 337
Tsp45I GTSAC 1 cut(s) 337
TspDTI ATGAA 3 cut(s) 96, 362, 368
TspGWI ACGGA 1 cut(s) 437
TspRI CASTG 3 cut(s) 342, 390, 453
Vha464I CTTAAG 1 cut(s) 314
VpaK11BI GGWCC 1 cut(s) 473
XbaI TCTAGA 1 cut(s) 22
XcmI CCANNNNNNNNNTGG 1 cut(s) 25
XmnI GAANNNNTTC 1 cut(s) 36
XspI CTAG 1 cut(s) 23
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.