MD02G1134200.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0000811
Physical Location & Seq
Reverse (-)
10877525 .. 10879685
2161 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1134200.v1.1.491

Sequence Viewer

Length: 642 bp
ATGGGAGCCGCAGAATCCACACTCTCAAGCTCACAGTGGCCGGCCGACGAAATCACCACCGTGTCGGAGCGACCAGAAACCGCCGATCCCGTCTTGGAAAAGCTCAAATCCCTTAAAATTTCGACGCCCCTACTGAGTTCGCCCCCGGAAGAGGGCAGCTTGACCGACATTTTAGTTAGGAAGCCTTCGTCTTCTTCAGTGCCAAGTGTTGTGGATCCGAAAGTGCTATTGGAACTCTTCTCGATGTATCGTGAGTGGGAGGAGGATAATGTCCAGAAGATGAGCAAAAGACAGGAAGAGATAGAAAATAAAATAGACGTTACGGATGCTTTGGCTGCTAAACTTCTTCAACGTTTTAATTATTCAGTTTCAGCAATGAAGACATCTTCACAACATTTATCGGAAGTCCATTCGTTGCAGGTGGAGATTGGGGAGCTTAAAGGAAGGTTAACAGAGGTCATTAGTAACCGCGATGCACTGTGCCGGAGAATATGTGTTGAAGGACCAGAATCCCTCCGGTCATCCATCAAGCCGTTTGCTGTCTGCACCACCGGCCAAGAAATCCAATCTAGCTCAAGCTCTTCCGATCTGCAAAGAGATCCGGACCGAAGCCTACCCTCTGCCGAAGCCAGGTTAGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

214

Amino Acids

23.51

Weight (kDa)

4.93

Isoelectric Point (pI)

68.97

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 409
Acc36I ACCTGC 1 cut(s) 409
AccII CGCG 1 cut(s) 471
AccIII TCCGGA 1 cut(s) 601
AciI CCGC 3 cut(s) 9, 81, 469
AclI AACGTT 1 cut(s) 352
AclWI GGATC 4 cut(s) 80, 209, 222, 593
AcoI YGGCCR 3 cut(s) 38, 42, 553
AcsI RAATTY 1 cut(s) 117
AcuI CTGAAG 1 cut(s) 180
AcyI GRCGYC 1 cut(s) 125
AfiI CCNNNNNNNGG 3 cut(s) 151, 152, 630
AgsI TTSAA 2 cut(s) 350, 500
AjnI CCWGG 1 cut(s) 629
AjuI GAANNNNNNNTTGG 2 cut(s) 212, 244
AleI CACNNNNGTG 1 cut(s) 59
AluBI AGCT 6 cut(s) 30, 103, 159, 436, 573, 579
AluI AGCT 6 cut(s) 30, 103, 159, 436, 573, 579
AlwI GGATC 4 cut(s) 80, 209, 222, 593
Aor13HI TCCGGA 1 cut(s) 601
AoxI GGCC 3 cut(s) 38, 42, 553
ApeKI GCWGC 2 cut(s) 156, 335
ApoI RAATTY 1 cut(s) 117
AspS9I GGNCC 2 cut(s) 503, 604
AsuC2I CCSGG 1 cut(s) 146
AsuHPI GGTGA 1 cut(s) 46
AvaII GGWCC 2 cut(s) 503, 604
BamHI GGATCC 1 cut(s) 214
BbsI GAAGAC 2 cut(s) 183, 386
BbvI GCAGC 2 cut(s) 168, 322
BccI CCATC 1 cut(s) 533
BceAI ACGGC 1 cut(s) 517
BciT130I CCWGG 1 cut(s) 631
BcnI CCSGG 1 cut(s) 146
BfaI CTAG 1 cut(s) 570
BfuAI ACCTGC 1 cut(s) 409
BisI GCNGC 3 cut(s) 9, 157, 336
BlsI GCNGC 3 cut(s) 10, 158, 337
Bme1390I CCNGG 2 cut(s) 146, 631
Bme18I GGWCC 2 cut(s) 503, 604
BmgT120I GGNCC 2 cut(s) 503, 604
BmiI GGNNCC 2 cut(s) 7, 216
BmrFI CCNGG 2 cut(s) 146, 631
BmsI GCATC 2 cut(s) 316, 463
BpiI GAAGAC 2 cut(s) 183, 386
BpuEI CTTGAG 2 cut(s) 10, 559
BpuMI CCSGG 1 cut(s) 146
BsaHI GRCGYC 1 cut(s) 125
BsaJI CCNNGG 1 cut(s) 144
BsaWI WCCGGW 2 cut(s) 516, 601
BsaXI ACNNNNNCTCC 2 cut(s) 478, 508
Bsc4I CCNNNNNNNGG 3 cut(s) 151, 152, 630
Bse118I RCCGGY 2 cut(s) 40, 551
Bse3DI GCAATG 1 cut(s) 381
BseAI TCCGGA 1 cut(s) 601
BseBI CCWGG 1 cut(s) 631
BseDI CCNNGG 1 cut(s) 144
BseGI GGATG 2 cut(s) 331, 521
BseLI CCNNNNNNNGG 3 cut(s) 151, 152, 630
BseMI GCAATG 1 cut(s) 381
BseMII CTCAG 1 cut(s) 125
BseRI GAGGAG 1 cut(s) 275
BseX3I CGGCCG 1 cut(s) 42
BseXI GCAGC 2 cut(s) 168, 322
BsgI GTGCAG 1 cut(s) 529
Bsh1236I CGCG 1 cut(s) 471
Bsh1285I CGRYCG 1 cut(s) 45
BshFI GGCC 3 cut(s) 40, 44, 555
BsiEI CGRYCG 1 cut(s) 45
BsiSI CCGG 6 cut(s) 41, 146, 484, 517, 552, 602
BslI CCNNNNNNNGG 3 cut(s) 151, 152, 630
BsnI GGCC 3 cut(s) 40, 44, 555
Bsp13I TCCGGA 1 cut(s) 601
Bsp143I GATC 4 cut(s) 85, 214, 586, 598
BspACI CCGC 3 cut(s) 9, 81, 469
BspANI GGCC 3 cut(s) 40, 44, 555
BspCNI CTCAG 1 cut(s) 126
BspEI TCCGGA 1 cut(s) 601
BspFNI CGCG 1 cut(s) 471
BspLI GGNNCC 2 cut(s) 7, 216
BspMI ACCTGC 1 cut(s) 409
BspPI GGATC 4 cut(s) 80, 209, 222, 593
BspQI GCTCTTC 1 cut(s) 586
BsrDI GCAATG 1 cut(s) 381
BsrFI RCCGGY 2 cut(s) 40, 551
BssAI RCCGGY 2 cut(s) 40, 551
BssECI CCNNGG 1 cut(s) 144
BssMI GATC 4 cut(s) 85, 214, 586, 598
BssNI GRCGYC 1 cut(s) 125
Bst2UI CCWGG 1 cut(s) 631
Bst4CI ACNGT 3 cut(s) 36, 61, 480
Bst6I CTCTTC 4 cut(s) 144, 242, 291, 586
BstACI GRCGYC 1 cut(s) 125
BstC8I GCNNGC 1 cut(s) 42
BstDEI CTNAG 1 cut(s) 134
BstF5I GGATG 2 cut(s) 331, 521
BstFNI CGCG 1 cut(s) 471
BstKTI GATC 4 cut(s) 88, 217, 589, 601
BstMBI GATC 4 cut(s) 85, 214, 586, 598
BstMCI CGRYCG 1 cut(s) 45
BstMWI GCNNNNNNNGC 2 cut(s) 335, 552
BstNI CCWGG 1 cut(s) 631
BstSCI CCNGG 2 cut(s) 144, 629
BstUI CGCG 1 cut(s) 471
BstV1I GCAGC 2 cut(s) 168, 322
BstV2I GAAGAC 2 cut(s) 183, 386
BstX2I RGATCY 2 cut(s) 214, 598
BstYI RGATCY 2 cut(s) 214, 598
BstZI CGGCCG 1 cut(s) 42
BsuRI GGCC 3 cut(s) 40, 44, 555
BtgZI GCGATG 1 cut(s) 486
BtsCI GGATG 2 cut(s) 331, 521
BtsIMutI CAGTG 3 cut(s) 41, 204, 476
BveI ACCTGC 1 cut(s) 409
Cac8I GCNNGC 1 cut(s) 42
Cfr10I RCCGGY 2 cut(s) 40, 551
Cfr13I GGNCC 2 cut(s) 503, 604
CpoI CGGWCCG 1 cut(s) 604
CseI GACGC 1 cut(s) 133
CspCI CAANNNNNGTGG 2 cut(s) 192, 227
CspI CGGWCCG 1 cut(s) 604
DdeI CTNAG 1 cut(s) 134
DpnI GATC 4 cut(s) 87, 216, 588, 600
DpnII GATC 4 cut(s) 85, 214, 586, 598
EaeI YGGCCR 3 cut(s) 38, 42, 553
EagI CGGCCG 1 cut(s) 42
Eam1104I CTCTTC 4 cut(s) 144, 242, 291, 586
EarI CTCTTC 4 cut(s) 144, 242, 291, 586
EclXI CGGCCG 1 cut(s) 42
Eco47I GGWCC 2 cut(s) 503, 604
Eco52I CGGCCG 1 cut(s) 42
Eco57I CTGAAG 1 cut(s) 180
EcoRII CCWGG 1 cut(s) 629
FaiI YATR 1 cut(s) 493
Fnu4HI GCNGC 3 cut(s) 9, 157, 336
FokI GGATG 2 cut(s) 338, 508
FseI GGCCGGCC 1 cut(s) 44
Fsp4HI GCNGC 3 cut(s) 9, 157, 336
FspBI CTAG 1 cut(s) 570
GluI GCNGC 3 cut(s) 9, 157, 336
HaeIII GGCC 3 cut(s) 40, 44, 555
HapII CCGG 6 cut(s) 41, 146, 484, 517, 552, 602
HgaI GACGC 1 cut(s) 133
Hin1I GRCGYC 1 cut(s) 125
HincII GTYRAC 1 cut(s) 450
HindII GTYRAC 1 cut(s) 450
HinfI GANTC 2 cut(s) 14, 509
HpaI GTTAAC 1 cut(s) 450
HpaII CCGG 6 cut(s) 41, 146, 484, 517, 552, 602
HphI GGTGA 1 cut(s) 46
Hpy166II GTNNAC 1 cut(s) 450
Hpy188I TCNGA 4 cut(s) 67, 219, 403, 586
Hpy188III TCNNGA 4 cut(s) 241, 251, 274, 602
Hpy8I GTNNAC 1 cut(s) 450
Hpy99I CGWCG 2 cut(s) 50, 127
HpyAV CCTTC 3 cut(s) 195, 438, 494
HpyCH4III ACNGT 3 cut(s) 36, 61, 480
HpyCH4IV ACGT 2 cut(s) 318, 352
HpyCH4V TGCA 4 cut(s) 418, 476, 546, 592
HpyF10VI GCNNNNNNNGC 2 cut(s) 335, 552
HpyF3I CTNAG 1 cut(s) 134
HpySE526I ACGT 2 cut(s) 318, 352
Hsp92I GRCGYC 1 cut(s) 125
Kpn2I TCCGGA 1 cut(s) 601
KroI GCCGGC 1 cut(s) 40
KroNI GCCGGC 1 cut(s) 42
KspAI GTTAAC 1 cut(s) 450
Kzo9I GATC 4 cut(s) 85, 214, 586, 598
LguI GCTCTTC 1 cut(s) 586
LmnI GCTCC 3 cut(s) 5, 67, 433
Lsp1109I GCAGC 2 cut(s) 168, 322
LweI GCATC 2 cut(s) 316, 463
MaeI CTAG 1 cut(s) 570
MaeII ACGT 2 cut(s) 318, 352
MaeIII GTNAC 2 cut(s) 319, 464
MalI GATC 4 cut(s) 87, 216, 588, 600
MboI GATC 4 cut(s) 85, 214, 586, 598
MflI RGATCY 2 cut(s) 214, 598
MluCI AATT 2 cut(s) 117, 358
MmeI TCCRAC 1 cut(s) 45
MnlI CCTC 6 cut(s) 145, 253, 256, 448, 524, 628
MroI TCCGGA 1 cut(s) 601
MroNI GCCGGC 1 cut(s) 40
MseI TTAA 4 cut(s) 114, 357, 438, 449
MslI CAYNNNNRTG 1 cut(s) 59
MspI CCGG 6 cut(s) 41, 146, 484, 517, 552, 602
MspR9I CCNGG 2 cut(s) 146, 631
MvaI CCWGG 1 cut(s) 631
MvnI CGCG 1 cut(s) 471
MwoI GCNNNNNNNGC 2 cut(s) 335, 552
NaeI GCCGGC 1 cut(s) 42
NciI CCSGG 1 cut(s) 146
NdeII GATC 4 cut(s) 85, 214, 586, 598
NgoMIV GCCGGC 1 cut(s) 40
NlaIV GGNNCC 2 cut(s) 7, 216
OliI CACNNNNGTG 1 cut(s) 59
PaqCI CACCTGC 1 cut(s) 409
PciSI GCTCTTC 1 cut(s) 586
PdiI GCCGGC 1 cut(s) 42
PfeI GAWTC 2 cut(s) 14, 509
PkrI GCNGC 3 cut(s) 10, 158, 337
Psp1406I AACGTT 1 cut(s) 352
Psp6I CCWGG 1 cut(s) 629
PspGI CCWGG 1 cut(s) 629
PspN4I GGNNCC 2 cut(s) 7, 216
PspPI GGNCC 2 cut(s) 503, 604
PsuI RGATCY 2 cut(s) 214, 598
RigI GGCCGGCC 1 cut(s) 44
RseI CAYNNNNRTG 1 cut(s) 59
Rsr2I CGGWCCG 1 cut(s) 604
RsrII CGGWCCG 1 cut(s) 604
SapI GCTCTTC 1 cut(s) 586
SaqAI TTAA 4 cut(s) 114, 357, 438, 449
SatI GCNGC 3 cut(s) 9, 157, 336
Sau3AI GATC 4 cut(s) 85, 214, 586, 598
Sau96I GGNCC 2 cut(s) 503, 604
ScrFI CCNGG 2 cut(s) 146, 631
SfaNI GCATC 2 cut(s) 316, 463
SinI GGWCC 2 cut(s) 503, 604
SmiMI CAYNNNNRTG 1 cut(s) 59
SmlI CTYRAG 2 cut(s) 25, 574
SmoI CTYRAG 2 cut(s) 25, 574
Sse9I AATT 2 cut(s) 117, 358
SsiI CCGC 3 cut(s) 9, 81, 469
SspMI CTAG 1 cut(s) 570
StyD4I CCNGG 2 cut(s) 144, 629
TaaI ACNGT 3 cut(s) 36, 61, 480
TaiI ACGT 2 cut(s) 321, 355
TaqI TCGA 2 cut(s) 122, 242
TaqII GACCGA 2 cut(s) 179, 621
TasI AATT 2 cut(s) 117, 358
TauI GCSGC 1 cut(s) 11
TfiI GAWTC 2 cut(s) 14, 509
Tru1I TTAA 4 cut(s) 114, 357, 438, 449
Tru9I TTAA 4 cut(s) 114, 357, 438, 449
TscAI CASTG 3 cut(s) 41, 204, 483
TseI GCWGC 2 cut(s) 156, 335
TspDTI ATGAA 1 cut(s) 392
TspGWI ACGGA 1 cut(s) 338
TspRI CASTG 3 cut(s) 41, 204, 483
VpaK11BI GGWCC 2 cut(s) 503, 604
XapI RAATTY 1 cut(s) 117
XspI CTAG 1 cut(s) 570
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.