Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0007152

Physical Location & Seq

Genomic Coordinates Reverse (-)

28273319 .. 28280838

Gene Structure 7520 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1187800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2901 bp

ATGGAGAACCCGCAGGCCCAGAAGCCATGGAAGGTGGAGTACGCGAAGTCGTCGAGATCGTCGTGCAAGACCTGCAAATCCCCCATTGAAAAGGAGACGCTCCGCCTCGGCAAGATGGTCCAGGCCACCCAATTCGACGGCGTCATGCCTATGTGGAACCATGCTGATTGCATAATGAAGAAATCGAACCAGATAAAATCAACTGATGACGTTGAAGGCCTTGAGCTCATTCGTTGGGAAGATCAGCAGAAAGTTAGAAGTTATGTACAAAGTGGTGGACCCCCGGATACAACACCAGCAACAAGCAAGTTCACTACTAAAGAATGTGGTATTGAAGTTTCGCCAACTTCTCGCGCTACTTGCAAGCAATGCAGCCAAAAGATTTTAAAAACAGAGGTCCGTATATCAACCAAGCCTGAAGGTCAAGGGCCTAGGAGCCTGGCATGGAACCATGCCAACTGCTTCATGGTATTATCACCATCCATCGAAGTGGAGAAGTTGCCTGGATGGGAGACCCTCCCAGTTTCTGATCAGGAAGCTGTACGTGCCTTGGTTAAGAAGGTTCCTTCTAATGCTGGCACAAAACGAAGAAAAGATGCTGGTGATGATCAGAAGTCAAAAGTTGCTAGGTCTGAAGGAGATGTGTCAATGAGCAGGGATGTGTCTGTGAGGAGTGCTTCTGATGTGGGGGATAAACTGGAGGCCCAAACTAAAGAGCTGTGGGCACTGAAAGATGATCTTAAAAAGCATGTGACAAATGCAGAGATGAGAGAAATGCTTGAAGCCAATGATCAAGATTCAACAGGATCAGAGCTTGATTTGCGCGAGCGCTGTGCTGATGGAATGATGTTTGGAGCACTTAAGAGCTGCCCACTCTGTTCTGGTTCTCTTCGATATTCTGGAGGCATGTACCGGTGCCATGGCTACCTATCGGCCTGGAGTAAGTGTTCTTACTCCACTCAGGAACCTGAACGTCTTGAAGGGAAGTGGAAAATCCCAGAAGATACGGAGAATCAATATCTTAAGAAGTGGTTTAAATCACAAAAGCTTGCAAAACCTGTGCGGATACTGCCTCCATTGACACCTAGCAAGCCTTCTGAGAGTCCAGGCCAATCTCATTCGTCTAACAGTACAAGCTTGGTGGATTTAAAAGTTGCTTTTCGCGGATTACCTAAAGAATCAATGGAAGAATGGAGTAGAAAAATTGACGGTGTAGCTGGAGTGGTTCATTCGAAGATCAAGAAAGATACTAATTGCTTAGTTGTGAGTGGAGCATTTGATGATGATGCTGAGATGAGAAAAGCCAGGAGGATGAAATTACCAATTGTTAGGGAAGATTATCTGGTTGATTGCTTTAAAAGACAGAAGAAGCTTCCATTTGATATGTACAAAGTTGAAGCTGTCGGTGAATCCTCTAGCATGGTCACTGTCAAAGTGAAAGGCCGAAGTGCTGTTCATGAATCTTCTGGTCTGCAAGATACTGGGCATATACTTGAGGATGGGAAAAGCATCTACAATACAACTTTAAGTATGTCTGATCTATCAACTGGTGTTAACAGTTACTACATTCTCCAAATTATACAAGATGATAAGAGTTCTGACTGTTATGTGTTCCGTAAATGGGGACGAGTGGGGAATGATAAAATTGGAGGAAACAAACTGGAAGATATGTCGAAATCAGATGCGATCCGTGAATTTAAACGTTTGTTCCTTGAGAAGACTGGGAATTCGTGGGAAGCATGGGAGCAAAAACAAAATTTTCAGAAGCAAGCTGGAAAATTCTTTCCATTGGATATTGATTATGGGATTAATAAACAGGTTTCCAGAAAAAATCAGAACAATGGGGACAGCAAATTGGCTCCTCAGTTAGCAGAATTGATGAAGATGCTTTTCAATGTTGAAACATACAGAGCTGCCATGATGGAATTTGAGATAAATATGTCAGAAATGCCGCTTGGAAAACTGAGTAAAGCCAACATCCAAAAGGGATTTGAGGCATTAACAGAAATACAGAATCTGTTGAATAATAATGGCCATGCTCCTTCCATAAAAGAAAGCTTGATTATAGATGCAAGCAACCGTTTTTTCACTGTGATCCCTTCCATACATCCACGTGTTATCAGGGATGAGGATGACTTTAAGTCAAAGGTGAAAATGTTAGAAGCTCTACAGGACATTGAAATTGCTTCCAGATTAGTTGGTTTTGATGCTGATAGTGATGACTCCCTTGATGAGAAGTATAAGAAGCTCCGGTGTGATATTGATCCAATTCCTCATGATAGTGAAGATTATCGATTGATTGAAAAATACCTTCTTACTACTCATGCTCCTACACATACGGATTGGAGTCTTGAACTTGAAGAAGTTTTTGCACTTGAAAGGGAAGGAGAATTTGATAAATTTGCTCCCTATCGGAAAAGGCTTAAGAACAGAATGCTTCTTTGGCATGGTTCTCGGTTTACAAATTTCGTGGGTATACTTAGCCAAGGACTAAGAATAGCTCCTCCTGAAGCTCCAGCTACTGGTTACATGTTCGGCAAGGGGATTTACTTTGCTGACCTTGTCAGCAAGAGTGCACAGTATTGCTATACAGATAAGAAAAATCCTGTGGGCCTCATGCTTCTAAGTGAAGTTGCATTGGGGGAGGTGTATGAGCTCAAGAAGGCTGCGTATATGGACAAGCCTCCTAAAGGAAAGCACTCTACAAAGGGGCTGGGCAAGAAAATACCTCAGGAGTCGGAGTATGTAAAATGGAAGGACAATGTCATCGTGCCTTGTGGCAAACCAGTGCCATCAAATGTCAAGGCCTCTGAGCTCATGTATAACGAGTACATCGTTTATGATACAGCTCAAGTTAAGATGCAATACCTGTTGAAGGTGAGGTTTCATCAAAAGAGGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0003824 GO:0003909 GO:0003910 GO:0003950 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006266 GO:0006271 GO:0006273 GO:0006281 GO:0006464 GO:0006471 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006979 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009719 GO:0009725 GO:0009737 GO:0009987 GO:0010033 GO:0016070 GO:0016740 GO:0016757 GO:0016763 GO:0016874 GO:0016886 GO:0019538 GO:0022616 GO:0033554 GO:0033993 GO:0034641 GO:0034645 GO:0036211 GO:0042221 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0046483 GO:0050896 GO:0051103 GO:0051716 GO:0071704 GO:0090304 GO:0097305 GO:0140097 GO:1901360 GO:1901564 GO:1901576 GO:1901700

KEGG Pathways

Metabolic & Signaling

ko03410 ko04210 ko04212 ko04214 ko04217 map03410 map04210 map04212 map04214 map04217

Pfam Domains

Protein Families

BRCT PADR1 PARP PARP_reg WGR zf-PARP

Protein Analysis

967

Amino Acids

109.34

Weight (kDa)

8.77

Isoelectric Point (pI)

42.47

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
zf-PARP	PF00645	13 - 88	1.1e-21	Poly(ADP-ribose) polymerase and DNA-Ligase Zn-finger region
zf-PARP	PF00645	112 - 184	7.8e-13	Poly(ADP-ribose) polymerase and DNA-Ligase Zn-finger region
PADR1_N	PF21728	232 - 285	8.9e-22	PADR1, N-terminal helical domain
Zn_ribbon_PADR1	PF08063	286 - 325	1.7e-16	PADR1 domain, zinc ribbon fold
BRCT	PF00533	380 - 452	2.7e-07	BRCA1 C Terminus (BRCT) domain
WGR	PF05406	505 - 583	2.3e-17	WGR domain
PARP_reg	PF02877	617 - 747	3.2e-37	Poly(ADP-ribose) polymerase, regulatory domain
PARP	PF00644	761 - 962	6.6e-82	Poly(ADP-ribose) polymerase catalytic domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0007152)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G31320
fragaria_vesca	FvH4_2g21980 FvH4_2g21980 FvH4_2g21980
malus_domestica	MD05G1201000.v1.1 MD10G1187800.v1.1
prunus_persica	Prupe.8G227600_v2.0.a1 Prupe.8G227600_v2.0.a1
pyrus_communis	pycom05g18780 pycom10g16230
rosa_chinensis	RchiOBHm_Chr6g0288861
rosa_laevigata	RLG00000012360
rosa_multiflora	Rmu_sc0005314.1_g000009
rosa_roxburghii	Rroxscaffold_7G00179830
rosa_rugosa	Rorug06G0198600 Rorug06G0198600 Rorug06G0198600 Rorug06G0198700 Rorug06G0198800 Rorug06G0198900 Rorug06G0198900
rosa_samantha	Rh6AG310000 Rh6BG315900 Rh6CG323400
rosa_wichuraiana	Rw6G026810

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	80
AccB1I	GGYRCC	1 cut(s)	915
AccB7I	CCANNNNNTGG	1 cut(s)	2522
AccI	GTMKAC	1 cut(s)	2476
AccII	CGCG	4 cut(s)	44, 354, 825, 1164
AciI	CCGC	5 cut(s)	11, 103, 1063, 1164, 1952
AclI	AACGTT	1 cut(s)	1702
AclWI	GGATC	4 cut(s)	814, 1681, 2089, 2258
AcoI	YGGCCR	1 cut(s)	2032
AcsI	RAATTY	8 cut(s)	1694, 1726, 1756, 1778, 1925, 2390, 2399, 2464
AcuI	CTGAAG	3 cut(s)	438, 654, 2529
AcvI	CACGTG	1 cut(s)	2114
AcyI	GRCGYC	1 cut(s)	141
AfaI	GTAC	7 cut(s)	41, 267, 543, 911, 1132, 1388, 2831
AfeI	AGCGCT	1 cut(s)	830
AfiI	CCNNNNNNNGG	4 cut(s)	1621, 2522, 2690, 2875
AflII	CTTAAG	3 cut(s)	860, 1022, 2423
AflIII	ACRYGT	2 cut(s)	2113, 2529
AgeI	ACCGGT	1 cut(s)	912
AhdI	GACNNNNNGTC	1 cut(s)	2140
AjnI	CCWGG	6 cut(s)	120, 438, 502, 935, 1105, 1304
AjuI	GAANNNNNNNTTGG	4 cut(s)	1938, 1970, 2425, 2457
AloI	GAACNNNNNNTCC	2 cut(s)	931, 963
Alw21I	GWGCWC	5 cut(s)	228, 859, 2578, 2658, 2817
Alw26I	GTCTC	2 cut(s)	89, 506
Alw44I	GTGCAC	1 cut(s)	2574
AlwI	GGATC	4 cut(s)	814, 1681, 2089, 2258
AlwNI	CAGNNNCTG	3 cut(s)	527, 2017, 2522
Aor51HI	AGCGCT	1 cut(s)	830
ApaLI	GTGCAC	1 cut(s)	2574
ApeKI	GCWGC	4 cut(s)	372, 867, 1913, 2666
ApoI	RAATTY	8 cut(s)	1694, 1726, 1756, 1778, 1925, 2390, 2399, 2464
ArsI	GACNNNNNNTTYG	2 cut(s)	1711, 1743
AseI	ATTAAT	1 cut(s)	1809
AsiGI	ACCGGT	1 cut(s)	912
AspA2I	CCTAGG	1 cut(s)	431
AspLEI	GCGC	3 cut(s)	356, 825, 831
AspS9I	GGNCC	7 cut(s)	16, 118, 278, 397, 428, 703, 2611
AsuC2I	CCSGG	1 cut(s)	284
AsuHPI	GGTGA	5 cut(s)	468, 614, 1418, 2161, 2890
AsuII	TTCGAA	1 cut(s)	1232
AvaII	GGWCC	3 cut(s)	118, 278, 397
AvrII	CCTAGG	1 cut(s)	431
AxyI	CCTNAGG	1 cut(s)	2730
BaeGI	GKGCMC	2 cut(s)	727, 2578
BalI	TGGCCA	1 cut(s)	2034
BanI	GGYRCC	1 cut(s)	915
BanII	GRGCYC	3 cut(s)	228, 2658, 2817
BarI	GAAGNNNNNNTAC	2 cut(s)	23, 55
BbrPI	CACGTG	1 cut(s)	2114
BbsI	GAAGAC	1 cut(s)	1724
Bbv12I	GWGCWC	5 cut(s)	228, 859, 2578, 2658, 2817
BbvI	GCAGC	4 cut(s)	384, 854, 1900, 2653
BccI	CCATC	8 cut(s)	109, 487, 491, 501, 833, 1493, 1915, 2800
BceAI	ACGGC	1 cut(s)	154
BcgI	CGANNNNNNTGC	4 cut(s)	815, 849, 2435, 2469
BciT130I	CCWGG	6 cut(s)	122, 440, 504, 937, 1107, 1306
BciVI	GTATCC	2 cut(s)	280, 1059
BclI	TGATCA	3 cut(s)	529, 607, 790
BcnI	CCSGG	1 cut(s)	284
BcoDI	GTCTC	2 cut(s)	89, 506
BfaI	CTAG	4 cut(s)	432, 627, 1086, 1416
BfmI	CTRYAG	1 cut(s)	2168
BfoI	RGCGCY	1 cut(s)	832
BfrI	CTTAAG	3 cut(s)	860, 1022, 2423
BfuAI	ACCTGC	1 cut(s)	80
BfuI	GTATCC	2 cut(s)	280, 1059
BisI	GCNGC	5 cut(s)	373, 868, 1914, 1952, 2667
BlnI	CCTAGG	1 cut(s)	431
BlsI	GCNGC	5 cut(s)	374, 869, 1915, 1953, 2668
Bme1390I	CCNGG	7 cut(s)	122, 284, 440, 504, 937, 1107, 1306
Bme18I	GGWCC	3 cut(s)	118, 278, 397
BmeRI	GACNNNNNGTC	1 cut(s)	2140
BmgT120I	GGNCC	7 cut(s)	16, 118, 278, 397, 428, 703, 2611
BmiI	GGNNCC	8 cut(s)	158, 280, 437, 449, 564, 917, 966, 1860
BmrFI	CCNGG	7 cut(s)	122, 284, 440, 504, 937, 1107, 1306
BmrI	ACTGGG	3 cut(s)	515, 1491, 1731
BmsI	GCATC	8 cut(s)	586, 1276, 1518, 1672, 1875, 2059, 2197, 2850
BmuI	ACTGGG	3 cut(s)	515, 1491, 1731
BpiI	GAAGAC	1 cut(s)	1724
BpmI	CTGGAG	5 cut(s)	719, 921, 958, 1239, 2499
Bpu14I	TTCGAA	1 cut(s)	1232
BpuEI	CTTGAG	5 cut(s)	242, 1514, 1733, 2642, 2835
BpuMI	CCSGG	1 cut(s)	284
Bsa29I	ATCGAT	1 cut(s)	2293
BsaAI	YACGTR	2 cut(s)	545, 2114
BsaBI	GATNNNNATC	1 cut(s)	2262
BsaHI	GRCGYC	1 cut(s)	141
BsaI	GGTCTC	1 cut(s)	506
BsaJI	CCNNGG	7 cut(s)	26, 106, 282, 431, 549, 919, 2485
BsaWI	WCCGGW	2 cut(s)	912, 2250
BsaXI	ACNNNNNCTCC	6 cut(s)	931, 961, 2311, 2341, 2726, 2756
Bsc4I	CCNNNNNNNGG	4 cut(s)	1621, 2522, 2690, 2875
Bse118I	RCCGGY	1 cut(s)	912
Bse1I	ACTGG	8 cut(s)	521, 702, 1486, 1552, 1665, 1726, 2527, 2786
Bse21I	CCTNAGG	1 cut(s)	2730
Bse3DI	GCAATG	1 cut(s)	374
Bse8I	GATNNNNATC	1 cut(s)	2262
BseBI	CCWGG	6 cut(s)	122, 440, 504, 937, 1107, 1306
BseCI	ATCGAT	1 cut(s)	2293
BseDI	CCNNGG	7 cut(s)	26, 106, 282, 431, 549, 919, 2485
BseGI	GGATG	9 cut(s)	479, 512, 664, 1317, 1504, 1977, 2107, 2131, 2137
BseJI	GATNNNNATC	1 cut(s)	2262
BseLI	CCNNNNNNNGG	4 cut(s)	1621, 2522, 2690, 2875
BseMI	GCAATG	1 cut(s)	374
BseMII	CTCAG	7 cut(s)	974, 1089, 1281, 1877, 1955, 2744, 2802
BseNI	ACTGG	8 cut(s)	521, 702, 1486, 1552, 1665, 1726, 2527, 2786
BseRI	GAGGAG	3 cut(s)	685, 1851, 2493
BseSI	GKGCMC	2 cut(s)	727, 2578
BseXI	GCAGC	4 cut(s)	384, 854, 1900, 2653
BseYI	CCCAGC	1 cut(s)	2713
Bsh1236I	CGCG	4 cut(s)	44, 354, 825, 1164
BshNI	GGYRCC	1 cut(s)	915
BshTI	ACCGGT	1 cut(s)	912
BshVI	ATCGAT	1 cut(s)	2293
BsiHKAI	GWGCWC	5 cut(s)	228, 859, 2578, 2658, 2817
BsiSI	CCGG	3 cut(s)	284, 913, 2251
BslFI	GGGAC	2 cut(s)	1638, 1859
BslI	CCNNNNNNNGG	4 cut(s)	1621, 2522, 2690, 2875
BsmAI	GTCTC	2 cut(s)	89, 506
BsmBI	CGTCTC	1 cut(s)	89
BsmFI	GGGAC	2 cut(s)	1638, 1859
BsmI	GAATGC	1 cut(s)	2439
Bso31I	GGTCTC	1 cut(s)	506
Bsp119I	TTCGAA	1 cut(s)	1232
Bsp1286I	GDGCHC	6 cut(s)	228, 727, 859, 2578, 2658, 2817
Bsp1407I	TGTACA	2 cut(s)	265, 1386
Bsp19I	CCATGG	2 cut(s)	26, 919
BspACI	CCGC	5 cut(s)	11, 103, 1063, 1164, 1952
BspCNI	CTCAG	7 cut(s)	973, 1090, 1282, 1876, 1956, 2743, 2803
BspDI	ATCGAT	1 cut(s)	2293
BspFNI	CGCG	4 cut(s)	44, 354, 825, 1164
BspHI	TCATGA	2 cut(s)	1456, 2275
BspLI	GGNNCC	8 cut(s)	158, 280, 437, 449, 564, 917, 966, 1860
BspMI	ACCTGC	1 cut(s)	80
BspPI	GGATC	4 cut(s)	814, 1681, 2089, 2258
BspT104I	TTCGAA	1 cut(s)	1232
BspT107I	GGYRCC	1 cut(s)	915
BspTI	CTTAAG	3 cut(s)	860, 1022, 2423
BspTNI	GGTCTC	1 cut(s)	506
BsrDI	GCAATG	1 cut(s)	374
BsrFI	RCCGGY	1 cut(s)	912
BsrGI	TGTACA	2 cut(s)	265, 1386
BsrI	ACTGG	8 cut(s)	521, 702, 1486, 1552, 1665, 1726, 2527, 2786
BssAI	RCCGGY	1 cut(s)	912
BssECI	CCNNGG	7 cut(s)	26, 106, 282, 431, 549, 919, 2485
BssNAI	GTATAC	1 cut(s)	2477
BssNI	GRCGYC	1 cut(s)	141
BssT1I	CCWWGG	5 cut(s)	26, 431, 549, 919, 2485
Bst1107I	GTATAC	1 cut(s)	2477
Bst2UI	CCWGG	6 cut(s)	122, 440, 504, 937, 1107, 1306
Bst4CI	ACNGT	8 cut(s)	1130, 1211, 1429, 1559, 1604, 2081, 2092, 2580
Bst6I	CTCTTC	1 cut(s)	894
BstACI	GRCGYC	1 cut(s)	141
BstAFI	CTTAAG	3 cut(s)	860, 1022, 2423
BstAPI	GCANNNNNTGC	2 cut(s)	72, 369
BstAUI	TGTACA	2 cut(s)	265, 1386
BstBAI	YACGTR	2 cut(s)	545, 2114
BstBI	TTCGAA	1 cut(s)	1232
BstC8I	GCNNGC	8 cut(s)	15, 365, 577, 827, 1050, 1091, 1770, 2074
BstDSI	CCRYGG	2 cut(s)	26, 919
BstENI	CCTNNNNNAGG	2 cut(s)	2688, 2873
BstF5I	GGATG	9 cut(s)	479, 512, 664, 1317, 1504, 1977, 2107, 2131, 2137
BstFNI	CGCG	4 cut(s)	44, 354, 825, 1164
BstH2I	RGCGCY	1 cut(s)	832
BstHHI	GCGC	3 cut(s)	356, 825, 831
BstMAI	GTCTC	2 cut(s)	89, 506
BstMWI	GCNNNNNNNGC	7 cut(s)	72, 360, 369, 545, 820, 1069, 2443
BstNI	CCWGG	6 cut(s)	122, 440, 504, 937, 1107, 1306
BstNSI	RCATGY	3 cut(s)	752, 910, 2533
BstSCI	CCNGG	7 cut(s)	120, 282, 438, 502, 935, 1105, 1304
BstSFI	CTRYAG	1 cut(s)	2168
BstSLI	GKGCMC	2 cut(s)	727, 2578
BstUI	CGCG	4 cut(s)	44, 354, 825, 1164
BstV1I	GCAGC	4 cut(s)	384, 854, 1900, 2653
BstV2I	GAAGAC	1 cut(s)	1724
BstXI	CCANNNNNNTGG	1 cut(s)	490
BstZ17I	GTATAC	1 cut(s)	2477
Bsu15I	ATCGAT	1 cut(s)	2293
Bsu36I	CCTNAGG	1 cut(s)	2730
BsuI	GTATCC	2 cut(s)	280, 1059
BsuTUI	ATCGAT	1 cut(s)	2293
BtgI	CCRYGG	2 cut(s)	26, 919
BtsCI	GGATG	9 cut(s)	479, 512, 664, 1317, 1504, 1977, 2107, 2131, 2137
BtsIMutI	CAGTG	4 cut(s)	725, 1425, 2088, 2793
BveI	ACCTGC	1 cut(s)	80
Cac8I	GCNNGC	8 cut(s)	15, 365, 577, 827, 1050, 1091, 1770, 2074
CaiI	CAGNNNCTG	3 cut(s)	527, 2017, 2522
CciI	TCATGA	2 cut(s)	1456, 2275
CfoI	GCGC	3 cut(s)	356, 825, 831
Cfr10I	RCCGGY	1 cut(s)	912
Cfr13I	GGNCC	7 cut(s)	16, 118, 278, 397, 428, 703, 2611
ClaI	ATCGAT	1 cut(s)	2293
CseI	GACGC	2 cut(s)	106, 130
Csp6I	GTAC	7 cut(s)	40, 266, 542, 910, 1131, 1387, 2830
CspAI	ACCGGT	1 cut(s)	912
CspCI	CAANNNNNGTGG	4 cut(s)	1122, 1157, 2451, 2486
CviQI	GTAC	7 cut(s)	40, 266, 542, 910, 1131, 1387, 2830
DraI	TTTAAA	5 cut(s)	387, 1036, 1149, 1357, 1699
DriI	GACNNNNNGTC	1 cut(s)	2140
EaeI	YGGCCR	1 cut(s)	2032
Eam1104I	CTCTTC	1 cut(s)	894
Eam1105I	GACNNNNNGTC	1 cut(s)	2140
EarI	CTCTTC	1 cut(s)	894
EciI	GGCGGA	1 cut(s)	92
Ecl136II	GAGCTC	3 cut(s)	226, 2656, 2815
Eco130I	CCWWGG	5 cut(s)	26, 431, 549, 919, 2485
Eco147I	AGGCCT	2 cut(s)	219, 2807
Eco24I	GRGCYC	3 cut(s)	228, 2658, 2817
Eco31I	GGTCTC	1 cut(s)	506
Eco47I	GGWCC	3 cut(s)	118, 278, 397
Eco47III	AGCGCT	1 cut(s)	830
Eco53kI	GAGCTC	3 cut(s)	226, 2656, 2815
Eco57I	CTGAAG	3 cut(s)	438, 654, 2529
Eco72I	CACGTG	1 cut(s)	2114
Eco81I	CCTNAGG	1 cut(s)	2730
EcoICRI	GAGCTC	3 cut(s)	226, 2656, 2815
EcoNI	CCTNNNNNAGG	2 cut(s)	2688, 2873
EcoO109I	RGGNCCY	1 cut(s)	428
EcoRI	GAATTC	1 cut(s)	1726
EcoRII	CCWGG	6 cut(s)	120, 438, 502, 935, 1105, 1304
EcoT14I	CCWWGG	5 cut(s)	26, 431, 549, 919, 2485
EcoT38I	GRGCYC	3 cut(s)	228, 2658, 2817
ErhI	CCWWGG	5 cut(s)	26, 431, 549, 919, 2485
Esp3I	CGTCTC	1 cut(s)	89
FalI	AAGNNNNNCTT	2 cut(s)	723, 755
FaqI	GGGAC	2 cut(s)	1638, 1859
FauI	CCCGC	1 cut(s)	18
FbaI	TGATCA	3 cut(s)	529, 607, 790
FblI	GTMKAC	1 cut(s)	2476
Fnu4HI	GCNGC	5 cut(s)	373, 868, 1914, 1952, 2667
FokI	GGATG	9 cut(s)	466, 519, 671, 1324, 1511, 1964, 2094, 2138, 2144
FriOI	GRGCYC	3 cut(s)	228, 2658, 2817
Fsp4HI	GCNGC	5 cut(s)	373, 868, 1914, 1952, 2667
FspBI	CTAG	4 cut(s)	432, 627, 1086, 1416
GlaI	GCGC	3 cut(s)	355, 824, 830
GluI	GCNGC	5 cut(s)	373, 868, 1914, 1952, 2667
GsaI	CCCAGC	1 cut(s)	2717
GsuI	CTGGAG	5 cut(s)	719, 921, 958, 1239, 2499
HaeII	RGCGCY	1 cut(s)	832
HapII	CCGG	3 cut(s)	284, 913, 2251
HgaI	GACGC	2 cut(s)	106, 130
HhaI	GCGC	3 cut(s)	356, 825, 831
Hin1I	GRCGYC	1 cut(s)	141
Hin6I	GCGC	3 cut(s)	354, 823, 829
HinP1I	GCGC	3 cut(s)	354, 823, 829
HincII	GTYRAC	1 cut(s)	1555
HindII	GTYRAC	1 cut(s)	1555
HindIII	AAGCTT	4 cut(s)	1046, 1135, 1370, 2056
HpaI	GTTAAC	1 cut(s)	1555
HpaII	CCGG	3 cut(s)	284, 913, 2251
HphI	GGTGA	5 cut(s)	468, 614, 1418, 2161, 2890
Hpy166II	GTNNAC	6 cut(s)	278, 312, 1555, 2460, 2477, 2576
Hpy8I	GTNNAC	6 cut(s)	278, 312, 1555, 2460, 2477, 2576
Hpy99I	CGWCG	3 cut(s)	55, 64, 140
HpyCH4III	ACNGT	8 cut(s)	1130, 1211, 1429, 1559, 1604, 2081, 2092, 2580
HpyCH4IV	ACGT	5 cut(s)	210, 544, 973, 1702, 2113
HpyF10VI	GCNNNNNNNGC	7 cut(s)	72, 360, 369, 545, 820, 1069, 2443
HpySE526I	ACGT	5 cut(s)	210, 544, 973, 1702, 2113
Hsp92I	GRCGYC	1 cut(s)	141
HspAI	GCGC	3 cut(s)	354, 823, 829
Ksp22I	TGATCA	3 cut(s)	529, 607, 790
KspAI	GTTAAC	1 cut(s)	1555
Lsp1109I	GCAGC	4 cut(s)	384, 854, 1900, 2653
LweI	GCATC	8 cut(s)	586, 1276, 1518, 1672, 1875, 2059, 2197, 2850
MaeI	CTAG	4 cut(s)	432, 627, 1086, 1416
MaeII	ACGT	5 cut(s)	210, 544, 973, 1702, 2113
MaeIII	GTNAC	4 cut(s)	751, 1423, 1559, 2525
MfeI	CAATTG	1 cut(s)	1323
MhlI	GDGCHC	6 cut(s)	228, 727, 859, 2578, 2658, 2817
MlsI	TGGCCA	1 cut(s)	2034
MluNI	TGGCCA	1 cut(s)	2034
MlyI	GAGTC	4 cut(s)	1111, 2216, 2356, 2744
MmeI	TCCRAC	1 cut(s)	2718
Mox20I	TGGCCA	1 cut(s)	2034
MscI	TGGCCA	1 cut(s)	2034
MslI	CAYNNNNRTG	4 cut(s)	149, 488, 2112, 2280
Msp20I	TGGCCA	1 cut(s)	2034
MspCI	CTTAAG	3 cut(s)	860, 1022, 2423
MspI	CCGG	3 cut(s)	284, 913, 2251
MspR9I	CCNGG	7 cut(s)	122, 284, 440, 504, 937, 1107, 1306
MunI	CAATTG	1 cut(s)	1323
Mva1269I	GAATGC	1 cut(s)	2439
MvaI	CCWGG	6 cut(s)	122, 440, 504, 937, 1107, 1306
MvnI	CGCG	4 cut(s)	44, 354, 825, 1164
MwoI	GCNNNNNNNGC	7 cut(s)	72, 360, 369, 545, 820, 1069, 2443
NciI	CCSGG	1 cut(s)	284
NcoI	CCATGG	2 cut(s)	26, 919
NlaIV	GGNNCC	8 cut(s)	158, 280, 437, 449, 564, 917, 966, 1860
NmeAIII	GCCGAG	1 cut(s)	87
NmuCI	GTSAC	2 cut(s)	751, 1423
NspI	RCATGY	3 cut(s)	752, 910, 2533
NspV	TTCGAA	1 cut(s)	1232
PagI	TCATGA	2 cut(s)	1456, 2275
PceI	AGGCCT	2 cut(s)	219, 2807
PciI	ACATGT	1 cut(s)	2529
PcsI	WCGNNNNNNNCGW	3 cut(s)	56, 59, 2832
PctI	GAATGC	1 cut(s)	2439
PfeI	GAWTC	6 cut(s)	797, 1012, 1178, 1409, 1460, 2014
PflFI	GACNNNGTC	3 cut(s)	140, 2561, 2762
PflMI	CCANNNNNTGG	1 cut(s)	2522
PinAI	ACCGGT	1 cut(s)	912
PkrI	GCNGC	5 cut(s)	374, 869, 1915, 1953, 2668
PleI	GAGTC	4 cut(s)	1110, 2216, 2355, 2743
PmaCI	CACGTG	1 cut(s)	2114
PmlI	CACGTG	1 cut(s)	2114
PpsI	GAGTC	4 cut(s)	1110, 2216, 2355, 2743
Ppu21I	YACGTR	2 cut(s)	545, 2114
PscI	ACATGT	1 cut(s)	2529
PshBI	ATTAAT	1 cut(s)	1809
Psp124BI	GAGCTC	3 cut(s)	228, 2658, 2817
Psp1406I	AACGTT	1 cut(s)	1702
Psp6I	CCWGG	6 cut(s)	120, 438, 502, 935, 1105, 1304
PspCI	CACGTG	1 cut(s)	2114
PspFI	CCCAGC	1 cut(s)	2713
PspGI	CCWGG	6 cut(s)	120, 438, 502, 935, 1105, 1304
PspN4I	GGNNCC	8 cut(s)	158, 280, 437, 449, 564, 917, 966, 1860
PspPI	GGNCC	7 cut(s)	16, 118, 278, 397, 428, 703, 2611
PstNI	CAGNNNCTG	3 cut(s)	527, 2017, 2522
PsyI	GACNNNGTC	3 cut(s)	140, 2561, 2762
RsaI	GTAC	7 cut(s)	41, 267, 543, 911, 1132, 1388, 2831
RsaNI	GTAC	7 cut(s)	40, 266, 542, 910, 1131, 1387, 2830
RseI	CAYNNNNRTG	4 cut(s)	149, 488, 2112, 2280
SacI	GAGCTC	3 cut(s)	228, 2658, 2817
SatI	GCNGC	5 cut(s)	373, 868, 1914, 1952, 2667
Sau96I	GGNCC	7 cut(s)	16, 118, 278, 397, 428, 703, 2611
SchI	GAGTC	4 cut(s)	1111, 2216, 2356, 2744
ScrFI	CCNGG	7 cut(s)	122, 284, 440, 504, 937, 1107, 1306
SduI	GDGCHC	6 cut(s)	228, 727, 859, 2578, 2658, 2817
SfaNI	GCATC	8 cut(s)	586, 1276, 1518, 1672, 1875, 2059, 2197, 2850
SfcI	CTRYAG	1 cut(s)	2168
SfuI	TTCGAA	1 cut(s)	1232
SinI	GGWCC	3 cut(s)	118, 278, 397
SmiMI	CAYNNNNRTG	4 cut(s)	149, 488, 2112, 2280
SmlI	CTYRAG	8 cut(s)	221, 860, 1022, 1493, 1712, 2423, 2657, 2850
SmoI	CTYRAG	8 cut(s)	221, 860, 1022, 1493, 1712, 2423, 2657, 2850
SseBI	AGGCCT	2 cut(s)	219, 2807
SsiI	CCGC	5 cut(s)	11, 103, 1063, 1164, 1952
SspMI	CTAG	4 cut(s)	432, 627, 1086, 1416
SstI	GAGCTC	3 cut(s)	228, 2658, 2817
StuI	AGGCCT	2 cut(s)	219, 2807
StyD4I	CCNGG	7 cut(s)	120, 282, 438, 502, 935, 1105, 1304
StyI	CCWWGG	5 cut(s)	26, 431, 549, 919, 2485
TaaI	ACNGT	8 cut(s)	1130, 1211, 1429, 1559, 1604, 2081, 2092, 2580
TaiI	ACGT	5 cut(s)	213, 547, 976, 1705, 2116
TaqI	TCGA	8 cut(s)	53, 135, 185, 486, 892, 1232, 1673, 2293
TatI	WGTACW	4 cut(s)	265, 1130, 1386, 2829
TauI	GCSGC	1 cut(s)	1954
TfiI	GAWTC	6 cut(s)	797, 1012, 1178, 1409, 1460, 2014
TscAI	CASTG	4 cut(s)	732, 1432, 2095, 2793
TseFI	GTSAC	2 cut(s)	751, 1423
TseI	GCWGC	4 cut(s)	372, 867, 1913, 2666
Tsp45I	GTSAC	2 cut(s)	751, 1423
TspDTI	ATGAA	8 cut(s)	191, 454, 1217, 1328, 1445, 1473, 1895, 2876
TspGWI	ACGGA	5 cut(s)	389, 1022, 1604, 1679, 2354
TspRI	CASTG	4 cut(s)	732, 1432, 2095, 2793
Tth111I	GACNNNGTC	3 cut(s)	140, 2561, 2762
Van91I	CCANNNNNTGG	1 cut(s)	2522
Vha464I	CTTAAG	3 cut(s)	860, 1022, 2423
VneI	GTGCAC	1 cut(s)	2574
VpaK11BI	GGWCC	3 cut(s)	118, 278, 397
VspI	ATTAAT	1 cut(s)	1809
XagI	CCTNNNNNAGG	2 cut(s)	2688, 2873
XapI	RAATTY	8 cut(s)	1694, 1726, 1756, 1778, 1925, 2390, 2399, 2464
XceI	RCATGY	3 cut(s)	752, 910, 2533
XcmI	CCANNNNNNNNNTGG	1 cut(s)	463
XmaJI	CCTAGG	1 cut(s)	431
XmiI	GTMKAC	1 cut(s)	2476
XspI	CTAG	4 cut(s)	432, 627, 1086, 1416

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.