Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0008494

Physical Location & Seq

Genomic Coordinates Forward (+)

33445282 .. 33449294

Gene Structure 4013 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1204900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1875 bp

ATGAAGCTGTTTCGTCGATGCCAGAGGATCCTCATCCTCGCTCTGCTATGCGTCTCTGTTTTCACGCCCGTCGTCTTCGTCTCCCAGAGGCTCAAAAATCTCACGCCTAATTGGCGGAACGAATCCATAGAGGATTTATCGAATATTAAATACAGGACTGATAGTGTGAAACTTAGCGTCGAGCAGGAATCGAGTGAAGGCTTGAAAGAGCCGAGAAAAGTTTTATATGAAGACACATATTTGCTTTCTGCAGTTAATTATACTCTTAAAAAGGATCATGTTGATAAGGAATCCGGAAATGTTGGGGACGCTACTGATATGTTGGAAAGAACTACAAATGGAACCAAATACGAGAGCAACAAAGGAGATAAGCAACTTCAGCAGAGACAGGTATCATCCATGTCTAGGGGAAAGAAGCAATTGAACAAACAAGATCAGAATGTACACTCTCAGTCGCGGAAAGTAATAGATGAGAGAATAAGACGGAAGAAAGATGTAAGACCTAATCGTGCAGTTCAACACGATCAGAATGCACATTCGAATTTACAGAAAGTTACAAAGAAGATAAATGAGATGAAGGATCAAGTGATTAGAGCTAAAGCATACTTGAGTTCTGCACCACCAAGCAGTAAGTCAAGTTTAGTGAAGGAGTTGAAACTGCAAATCAAAGAGGTGGAACGAGCAATTGGTGGAGCCACCAAGGATTCGGATCTATCAAAGAGTGCCTTACAGAGAATGAGAAAGATGGAGTCTTCACTATCTAAAGCCAGGCGTACATACCCAGACTGCTCTTCCATGTCCAAAAAGCTTCGTGCTATGACTCAGAATGCTGAAGAGCAAATTCAGTCGCAGAAGAAGCAAGTCTCTTTTCTTTTCAGCCTCGCTTCAAGCACAACCCCAAAAGGCCTTCATTGTCTTTCTATGCAGCTGACTGCTGAATACTTTGCTCTGGCTCCCAAGGACAGGCAGTTTCCTAACCAGCAAAAGTTGCATGATTCAAAGCTCTATCATTATGCTGTTTTCTCCGACAATGTTCTGGGATGTACAGTGGTTGTGAACTCCACTGTTTCCACTGCCTTGAAACCAGAGAAGATTGTTTTCCATGTTGTGACTGATGCACTCAATTTTCCAGCAATCTCAATGTGGTTCTTATTAAATCCTCCTGGAAAAGCCACAATCCAAGTCCAGAGCATTGATAGTTTTGAATGGTTGTCTACCAAGTACAATACAAGTTTAAGGCAAAACTCCAGTGACCCTAGATATTCTTCCGAACTCAACCACCTCCGTTTCTACCTGCCAGATATCTTCCCAGCATTGAATAAGATTGTGCTCTTTGACCATGATGTGGTGGTGCAAAAGGATCTAAGTAAGCTATGGAGTGTTGATATGAAAGGAAAAGTAAATGGAGCAGTTGAGACTTGTCGGGAGAGTGAAACTTCCTTCCTTCGGATGGATACATTCATCAACTTCTCAGATCCATTTGTGGCAAAAAGGTTTGATGCCAATGCATGCACATGGGCATTTGGGATGAATTTGTTTGACCTTCAAGAATGGAGGAGAAAAGATCTGACTACACTCTACGACAAGTACTTGAAATCGGGTTACAAGAGGCCGTTGTGGGTGGCTGGGAGTTTGCCCTTAGGTTGGATCACCTTCTATAACCAGACGGTAGCTTTGGACAGGCGGTGGCACATCCTTGGGCTGGGTTATAACTCAGGTGTGGTTCGTGCTGACATTGATCGAGCGGCAGTAATACACTATGATGGAATCATGAAGCCATGGTTGGACATAGCGATTGCGAGGTACAAGGATTATTGGAGAAAATATGTCAACTATGACGACTCTTATCTGCAACGGTGCAACATTCATGCATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000139 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005794 GO:0012505 GO:0016020 GO:0031090 GO:0031984 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0098588 GO:0098791

KEGG Pathways

Metabolic & Signaling

ko00520 map00520

Pfam Domains

Protein Families

Glyco_transf_8

Protein Analysis

625

Amino Acids

71.84

Weight (kDa)

9.57

Isoelectric Point (pI)

46.33

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GAUT_1	PF25557	187 - 260	7.6e-27	Galacturonosyltransferase domain 1
Glyco_transf_8	PF01501	305 - 598	1.2e-62	Glycosyl transferase family 8

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008494)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G06780 AT1G06780 AT2G30575
fragaria_vesca	FvH4_2g22460 FvH4_2g22460
malus_domestica	MD05G1204900.v1.1 MD10G1192000.v1.1
prunus_persica	Prupe.8G231500_v2.0.a1 Prupe.8G231500_v2.0.a1
pyrus_communis	pycom05g19110 pycom10g16590
rosa_chinensis	RchiOBHm_Chr6g0289401
rosa_laevigata	RLG00000012307
rosa_multiflora	Rmu_co8414935.1_g000001
rosa_roxburghii	Rroxscaffold_7G00179240
rosa_rugosa	Rorug06G0203100 Rorug06G0203200 Rorug06G0203300
rosa_samantha	Rh6AG314200 Rh6BG321800 Rh6CG328000 Rh6DG313700
rosa_wichuraiana	Rw6G027160

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1710
Acc36I	ACCTGC	1 cut(s)	1302
AccB7I	CCANNNNNTGG	1 cut(s)	1345
AccBSI	CCGCTC	1 cut(s)	1745
AccI	GTMKAC	1 cut(s)	1214
AccII	CGCG	1 cut(s)	457
AccIII	TCCGGA	1 cut(s)	293
AciI	CCGC	4 cut(s)	115, 457, 1684, 1745
AclWI	GGATC	8 cut(s)	22, 35, 282, 588, 717, 1368, 1469, 1655
AcsI	RAATTY	3 cut(s)	541, 840, 1531
AcuI	CTGAAG	2 cut(s)	362, 852
AfaI	GTAC	6 cut(s)	444, 775, 1045, 1223, 1589, 1805
AfiI	CCNNNNNNNGG	7 cut(s)	405, 963, 1345, 1446, 1450, 1644, 1702
AjnI	CCWGG	2 cut(s)	767, 1162
AjuI	GAANNNNNNNTTGG	4 cut(s)	669, 701, 1766, 1798
AluBI	AGCT	7 cut(s)	7, 596, 808, 928, 1003, 1372, 1673
AluI	AGCT	7 cut(s)	7, 596, 808, 928, 1003, 1372, 1673
Alw21I	GWGCWC	1 cut(s)	1332
Alw26I	GTCTC	5 cut(s)	58, 85, 379, 868, 1409
AlwI	GGATC	8 cut(s)	22, 35, 282, 588, 717, 1368, 1469, 1655
Aor13HI	TCCGGA	1 cut(s)	293
AoxI	GGCC	2 cut(s)	904, 1610
ApeKI	GCWGC	1 cut(s)	925
ApoI	RAATTY	3 cut(s)	541, 840, 1531
ArsI	GACNNNNNNTTYG	2 cut(s)	1657, 1689
AsuHPI	GGTGA	1 cut(s)	1642
AsuII	TTCGAA	1 cut(s)	539
AxyI	CCTNAGG	1 cut(s)	1639
BamHI	GGATCC	1 cut(s)	27
BbsI	GAAGAC	3 cut(s)	67, 237, 744
Bbv12I	GWGCWC	1 cut(s)	1332
BbvI	GCAGC	1 cut(s)	937
BccI	CCATC	3 cut(s)	739, 1444, 1757
BceAI	ACGGC	1 cut(s)	1597
BcgI	CGANNNNNNTGC	2 cut(s)	512, 546
BciT130I	CCWGG	2 cut(s)	769, 1164
BciVI	GTATCC	1 cut(s)	1447
BcoDI	GTCTC	5 cut(s)	58, 85, 379, 868, 1409
BfaI	CTAG	2 cut(s)	405, 1257
BfmI	CTRYAG	1 cut(s)	249
BfuAI	ACCTGC	1 cut(s)	1302
BfuI	GTATCC	1 cut(s)	1447
BglI	GCCNNNNNGGC	1 cut(s)	112
BglII	AGATCT	1 cut(s)	1564
BisI	GCNGC	2 cut(s)	926, 1746
BlsI	GCNGC	2 cut(s)	927, 1747
BmcAI	AGTACT	1 cut(s)	1589
Bme1390I	CCNGG	2 cut(s)	769, 1164
BmiI	GGNNCC	4 cut(s)	29, 343, 694, 954
BmrFI	CCNGG	2 cut(s)	769, 1164
BmsI	GCATC	3 cut(s)	8, 1105, 1489
BpiI	GAAGAC	3 cut(s)	67, 237, 744
BpmI	CTGGAG	1 cut(s)	1231
Bpu14I	TTCGAA	1 cut(s)	539
BpuEI	CTTGAG	1 cut(s)	628
BsaBI	GATNNNNATC	2 cut(s)	32, 708
BsaJI	CCNNGG	4 cut(s)	699, 957, 1696, 1778
BsaWI	WCCGGW	1 cut(s)	293
Bsc4I	CCNNNNNNNGG	7 cut(s)	405, 963, 1345, 1446, 1450, 1644, 1702
Bse1I	ACTGG	1 cut(s)	1248
Bse21I	CCTNAGG	1 cut(s)	1639
Bse8I	GATNNNNATC	2 cut(s)	32, 708
BseAI	TCCGGA	1 cut(s)	293
BseBI	CCWGG	2 cut(s)	769, 1164
BseDI	CCNNGG	4 cut(s)	699, 957, 1696, 1778
BseGI	GGATG	6 cut(s)	33, 395, 1046, 1455, 1533, 1692
BseJI	GATNNNNATC	2 cut(s)	32, 708
BseLI	CCNNNNNNNGG	7 cut(s)	405, 963, 1345, 1446, 1450, 1644, 1702
BseMII	CTCAG	4 cut(s)	464, 836, 1485, 1728
BseNI	ACTGG	1 cut(s)	1248
BseRI	GAGGAG	1 cut(s)	1570
BseXI	GCAGC	1 cut(s)	937
BseYI	CCCAGC	3 cut(s)	1309, 1625, 1702
BsgI	GTGCAG	2 cut(s)	531, 600
Bsh1236I	CGCG	1 cut(s)	457
BshFI	GGCC	2 cut(s)	906, 1612
BsiHKAI	GWGCWC	1 cut(s)	1332
BsiSI	CCGG	1 cut(s)	294
BslFI	GGGAC	1 cut(s)	320
BslI	CCNNNNNNNGG	7 cut(s)	405, 963, 1345, 1446, 1450, 1644, 1702
BsmAI	GTCTC	5 cut(s)	58, 85, 379, 868, 1409
BsmBI	CGTCTC	2 cut(s)	58, 85
BsmFI	GGGAC	1 cut(s)	320
BsmI	GAATGC	2 cut(s)	535, 832
BsnI	GGCC	2 cut(s)	906, 1612
Bsp119I	TTCGAA	1 cut(s)	539
Bsp1286I	GDGCHC	1 cut(s)	1332
Bsp13I	TCCGGA	1 cut(s)	293
Bsp1407I	TGTACA	2 cut(s)	442, 1043
Bsp19I	CCATGG	1 cut(s)	1778
BspACI	CCGC	4 cut(s)	115, 457, 1684, 1745
BspANI	GGCC	2 cut(s)	906, 1612
BspCNI	CTCAG	4 cut(s)	463, 835, 1484, 1727
BspEI	TCCGGA	1 cut(s)	293
BspFNI	CGCG	1 cut(s)	457
BspHI	TCATGA	1 cut(s)	1770
BspLI	GGNNCC	4 cut(s)	29, 343, 694, 954
BspMAI	CTGCAG	1 cut(s)	253
BspMI	ACCTGC	1 cut(s)	1302
BspPI	GGATC	8 cut(s)	22, 35, 282, 588, 717, 1368, 1469, 1655
BspQI	GCTCTTC	2 cut(s)	796, 828
BspT104I	TTCGAA	1 cut(s)	539
BsrBI	CCGCTC	1 cut(s)	1745
BsrGI	TGTACA	2 cut(s)	442, 1043
BsrI	ACTGG	1 cut(s)	1248
BssECI	CCNNGG	4 cut(s)	699, 957, 1696, 1778
BssT1I	CCWWGG	4 cut(s)	699, 957, 1696, 1778
Bst2UI	CCWGG	2 cut(s)	769, 1164
Bst4CI	ACNGT	4 cut(s)	1048, 1066, 1669, 1857
Bst6I	CTCTTC	2 cut(s)	796, 828
BstAPI	GCANNNNNTGC	1 cut(s)	988
BstAUI	TGTACA	2 cut(s)	442, 1043
BstBI	TTCGAA	1 cut(s)	539
BstC8I	GCNNGC	1 cut(s)	1510
BstDEI	CTNAG	7 cut(s)	173, 450, 822, 1364, 1471, 1639, 1714
BstDSI	CCRYGG	1 cut(s)	1778
BstF5I	GGATG	6 cut(s)	33, 395, 1046, 1455, 1533, 1692
BstFNI	CGCG	1 cut(s)	457
BstMAI	GTCTC	5 cut(s)	58, 85, 379, 868, 1409
BstMWI	GCNNNNNNNGC	4 cut(s)	112, 379, 856, 988
BstNI	CCWGG	2 cut(s)	769, 1164
BstNSI	RCATGY	1 cut(s)	1512
BstSCI	CCNGG	2 cut(s)	767, 1162
BstSFI	CTRYAG	1 cut(s)	249
BstUI	CGCG	1 cut(s)	457
BstV1I	GCAGC	1 cut(s)	937
BstV2I	GAAGAC	3 cut(s)	67, 237, 744
BstX2I	RGATCY	5 cut(s)	27, 709, 1360, 1474, 1564
BstYI	RGATCY	5 cut(s)	27, 709, 1360, 1474, 1564
Bsu36I	CCTNAGG	1 cut(s)	1639
BsuI	GTATCC	1 cut(s)	1447
BsuRI	GGCC	2 cut(s)	906, 1612
BtgI	CCRYGG	1 cut(s)	1778
BtsCI	GGATG	6 cut(s)	33, 395, 1046, 1455, 1533, 1692
BtsI	GCAGTG	1 cut(s)	1071
BtsIMutI	CAGTG	4 cut(s)	1053, 1062, 1071, 1255
BveI	ACCTGC	1 cut(s)	1302
Cac8I	GCNNGC	1 cut(s)	1510
CciI	TCATGA	1 cut(s)	1770
CseI	GACGC	3 cut(s)	40, 166, 317
Csp6I	GTAC	6 cut(s)	443, 774, 1044, 1222, 1588, 1804
CviQI	GTAC	6 cut(s)	443, 774, 1044, 1222, 1588, 1804
DdeI	CTNAG	7 cut(s)	173, 450, 822, 1364, 1471, 1639, 1714
Eam1104I	CTCTTC	2 cut(s)	796, 828
EarI	CTCTTC	2 cut(s)	796, 828
EciI	GGCGGA	1 cut(s)	130
Eco130I	CCWWGG	4 cut(s)	699, 957, 1696, 1778
Eco147I	AGGCCT	1 cut(s)	906
Eco32I	GATATC	1 cut(s)	1303
Eco57I	CTGAAG	2 cut(s)	362, 852
Eco81I	CCTNAGG	1 cut(s)	1639
EcoRII	CCWGG	2 cut(s)	767, 1162
EcoRV	GATATC	1 cut(s)	1303
EcoT14I	CCWWGG	4 cut(s)	699, 957, 1696, 1778
EcoT22I	ATGCAT	2 cut(s)	1510, 1873
ErhI	CCWWGG	4 cut(s)	699, 957, 1696, 1778
Esp3I	CGTCTC	2 cut(s)	58, 85
FalI	AAGNNNNNCTT	2 cut(s)	710, 742
FaqI	GGGAC	1 cut(s)	320
FblI	GTMKAC	1 cut(s)	1214
Fnu4HI	GCNGC	2 cut(s)	926, 1746
FokI	GGATG	6 cut(s)	20, 382, 1053, 1462, 1540, 1679
Fsp4HI	GCNGC	2 cut(s)	926, 1746
FspBI	CTAG	2 cut(s)	405, 1257
GluI	GCNGC	2 cut(s)	926, 1746
GsaI	CCCAGC	3 cut(s)	1313, 1629, 1706
GsuI	CTGGAG	1 cut(s)	1231
HaeIII	GGCC	2 cut(s)	906, 1612
HapII	CCGG	1 cut(s)	294
HgaI	GACGC	3 cut(s)	40, 166, 317
HincII	GTYRAC	1 cut(s)	1831
HindII	GTYRAC	1 cut(s)	1831
HindIII	AAGCTT	1 cut(s)	806
HinfI	GANTC	9 cut(s)	122, 188, 290, 704, 749, 820, 995, 1767, 1841
HpaII	CCGG	1 cut(s)	294
HphI	GGTGA	1 cut(s)	1642
Hpy166II	GTNNAC	4 cut(s)	445, 1057, 1215, 1831
Hpy188I	TCNGA	9 cut(s)	438, 528, 709, 825, 1027, 1270, 1449, 1474, 1569
Hpy188III	TCNNGA	5 cut(s)	294, 1186, 1424, 1547, 1771
Hpy8I	GTNNAC	4 cut(s)	445, 1057, 1215, 1831
Hpy99I	CGWCG	3 cut(s)	18, 74, 182
HpyAV	CCTTC	8 cut(s)	191, 571, 640, 917, 1450, 1454, 1553, 1663
HpyCH4III	ACNGT	4 cut(s)	1048, 1066, 1669, 1857
HpyF10VI	GCNNNNNNNGC	4 cut(s)	112, 379, 856, 988
HpyF3I	CTNAG	7 cut(s)	173, 450, 822, 1364, 1471, 1639, 1714
Kpn2I	TCCGGA	1 cut(s)	293
LguI	GCTCTTC	2 cut(s)	796, 828
LmnI	GCTCC	3 cut(s)	692, 958, 1406
Lsp1109I	GCAGC	1 cut(s)	937
LweI	GCATC	3 cut(s)	8, 1105, 1489
MaeI	CTAG	2 cut(s)	405, 1257
MaeIII	GTNAC	4 cut(s)	553, 1108, 1250, 1601
MbiI	CCGCTC	1 cut(s)	1745
MfeI	CAATTG	2 cut(s)	419, 684
MflI	RGATCY	5 cut(s)	27, 709, 1360, 1474, 1564
MhlI	GDGCHC	1 cut(s)	1332
MluCI	AATT	8 cut(s)	109, 256, 419, 541, 684, 840, 1123, 1531
MlyI	GAGTC	3 cut(s)	758, 814, 1835
MmeI	TCCRAC	4 cut(s)	303, 1050, 1625, 1764
Mph1103I	ATGCAT	2 cut(s)	1510, 1873
MroI	TCCGGA	1 cut(s)	293
MseI	TTAA	5 cut(s)	147, 255, 267, 1154, 1235
MslI	CAYNNNNRTG	2 cut(s)	1513, 1761
MspA1I	CMGCKG	1 cut(s)	928
MspI	CCGG	1 cut(s)	294
MspR9I	CCNGG	2 cut(s)	769, 1164
MunI	CAATTG	2 cut(s)	419, 684
Mva1269I	GAATGC	2 cut(s)	535, 832
MvaI	CCWGG	2 cut(s)	769, 1164
MvnI	CGCG	1 cut(s)	457
MwoI	GCNNNNNNNGC	4 cut(s)	112, 379, 856, 988
NcoI	CCATGG	1 cut(s)	1778
NlaIV	GGNNCC	4 cut(s)	29, 343, 694, 954
NmeAIII	GCCGAG	1 cut(s)	237
NmuCI	GTSAC	2 cut(s)	1108, 1250
NsiI	ATGCAT	2 cut(s)	1510, 1873
NspI	RCATGY	1 cut(s)	1512
NspV	TTCGAA	1 cut(s)	539
PaeI	GCATGC	1 cut(s)	1512
PagI	TCATGA	1 cut(s)	1770
PceI	AGGCCT	1 cut(s)	906
PciSI	GCTCTTC	2 cut(s)	796, 828
PctI	GAATGC	2 cut(s)	535, 832
PfeI	GAWTC	6 cut(s)	122, 188, 290, 704, 995, 1767
PflMI	CCANNNNNTGG	1 cut(s)	1345
PfoI	TCCNGGA	1 cut(s)	1162
PkrI	GCNGC	2 cut(s)	927, 1747
PleI	GAGTC	3 cut(s)	757, 814, 1835
PpsI	GAGTC	3 cut(s)	757, 814, 1835
PsiI	TTATAA	1 cut(s)	1710
Psp6I	CCWGG	2 cut(s)	767, 1162
PspFI	CCCAGC	3 cut(s)	1309, 1625, 1702
PspGI	CCWGG	2 cut(s)	767, 1162
PspN4I	GGNNCC	4 cut(s)	29, 343, 694, 954
PstI	CTGCAG	1 cut(s)	253
PsuI	RGATCY	5 cut(s)	27, 709, 1360, 1474, 1564
PvuII	CAGCTG	1 cut(s)	928
RsaI	GTAC	6 cut(s)	444, 775, 1045, 1223, 1589, 1805
RsaNI	GTAC	6 cut(s)	443, 774, 1044, 1222, 1588, 1804
RseI	CAYNNNNRTG	2 cut(s)	1513, 1761
SapI	GCTCTTC	2 cut(s)	796, 828
SaqAI	TTAA	5 cut(s)	147, 255, 267, 1154, 1235
SatI	GCNGC	2 cut(s)	926, 1746
ScaI	AGTACT	1 cut(s)	1589
SchI	GAGTC	3 cut(s)	758, 814, 1835
ScrFI	CCNGG	2 cut(s)	769, 1164
SduI	GDGCHC	1 cut(s)	1332
SfaNI	GCATC	3 cut(s)	8, 1105, 1489
SfcI	CTRYAG	1 cut(s)	249
SfuI	TTCGAA	1 cut(s)	539
SmiMI	CAYNNNNRTG	2 cut(s)	1513, 1761
SmlI	CTYRAG	1 cut(s)	607
SmoI	CTYRAG	1 cut(s)	607
SphI	GCATGC	1 cut(s)	1512
Sse9I	AATT	8 cut(s)	109, 256, 419, 541, 684, 840, 1123, 1531
SseBI	AGGCCT	1 cut(s)	906
SsiI	CCGC	4 cut(s)	115, 457, 1684, 1745
SspI	AATATT	1 cut(s)	145
SspMI	CTAG	2 cut(s)	405, 1257
StuI	AGGCCT	1 cut(s)	906
StyD4I	CCNGG	2 cut(s)	767, 1162
StyI	CCWWGG	4 cut(s)	699, 957, 1696, 1778
TaaI	ACNGT	4 cut(s)	1048, 1066, 1669, 1857
TaqI	TCGA	6 cut(s)	16, 140, 180, 191, 539, 1741
TasI	AATT	8 cut(s)	109, 256, 419, 541, 684, 840, 1123, 1531
TatI	WGTACW	4 cut(s)	442, 1043, 1221, 1587
TauI	GCSGC	1 cut(s)	1748
TfiI	GAWTC	6 cut(s)	122, 188, 290, 704, 995, 1767
Tru1I	TTAA	5 cut(s)	147, 255, 267, 1154, 1235
Tru9I	TTAA	5 cut(s)	147, 255, 267, 1154, 1235
TscAI	CASTG	4 cut(s)	1053, 1069, 1078, 1255
TseFI	GTSAC	2 cut(s)	1108, 1250
TseI	GCWGC	1 cut(s)	925
Tsp45I	GTSAC	2 cut(s)	1108, 1250
TspDTI	ATGAA	9 cut(s)	17, 243, 590, 899, 1403, 1450, 1544, 1787, 1856
TspGWI	ACGGA	2 cut(s)	499, 1274
TspRI	CASTG	4 cut(s)	1053, 1069, 1078, 1255
Van91I	CCANNNNNTGG	1 cut(s)	1345
XapI	RAATTY	3 cut(s)	541, 840, 1531
XceI	RCATGY	1 cut(s)	1512
XmiI	GTMKAC	1 cut(s)	1214
XspI	CTAG	2 cut(s)	405, 1257
ZrmI	AGTACT	1 cut(s)	1589
Zsp2I	ATGCAT	2 cut(s)	1510, 1873

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.