Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0008494

Physical Location & Seq

Genomic Coordinates Forward (+)

28854446 .. 28858421

Gene Structure 3976 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1192000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1875 bp

ATGAAGCTGTTTCGTCGATGCCAGAGGATCCTCATCCTCGCTCTGCTCTGCGTCTCTGTTTTCGCGCCCATCGTCTTCGTCTCCCAGAGACTAAAAAATGTCATGCCTACTGGGCGGGAGGAGTTCATAGAGGATTTGTCGAATATTAAATACCGGACTGATAGTGTGAAGCTTAGCGTCGAGCAGGAATCGAGTGAAAGCTTGAAAGAGCCGAGAAAAGTTTTATACGAAGATGCAGATTTGCTTTCCACAGTTAATTATACTCGTAAAGAGATTAATGTTGATAAGGAATCCGGAAATGTTAGGGACGCTACTGATATGTTGGAAAGAACTGGAGACGGAATCAAATATGGGAGCAACAAAGGAGATAAGCAACTTCAGCTGAGAGAAATATTAACCATGTCTGGGGAAAAGAAGCAATTGAACAAACAGGATCAGAATGTACACTCTCAGTCAGGGGAAGTAATAGATGAGAGAATAAGACAGATGAAAGACGTAAAACCTAATCGTGCAGTTCAACACGATCAGAATGCACATTTAAATTTAAAGAAAGTTACAGAGAAGATAAATGAGATGAAAGATCAAGTGATTAGAGCTAAAGCATACTTGAGTTTTGCACCACCAGGCAGTAAGTCAAGTTTAGTGAAGGAGTTGAAACTGCGAATTAAAGAGGTAGAACGAGCAATTGGTGGAGCCACCAAGGATTCAGATTTATCAAGGAGTGCCTTGCAGAGAATGAGAAACATGGAGTCTTCACTATCTAAAGCCAGCCATACATACCCAGATTGCTCTTCCATGTCCACAAAGCTCCGTGCTATGACTCAAAATGCTGAAGAGCAAGTTCAGTCGCAGAAGAAGCAAGTCTCTTTTCTGTTCAACCTCGCTGCAAGCACAGCCCCGAAAGGTCTACATTGTCTTTCTATGCAGCTGACTGCAGAATACTTTGCTCTGGATTCCAAGGAGAGGCAGTTTCCTAACCAGAAAAAGTTGCATGATTCAAAGCTCTATCATTATGCTGTTTTCTCCAACAATGTTCTGGCATGTACAGTGGTTGTGAACTCCACTGTTTCCACTGCCTTGAAACCAGAGAAGATTGTTTTCCATGTGGTGACTGATGCACTCAATTTTCCAGCAATCTCAATGTGGTTCTTATTAAATCCTCCTGGAAAAGCCACAATCCAAGTCCAGAGCATAGATAGTTTCAAATGGTTGTCGACCAAGTACAATACAAGATTTAGGCAAAACTCCAGTGACCCTAGGTATACTTCCGAACTCAACCACCTCCGTTTCTATCTGCCATATATCTTCCCGGCATTGAAAAAGATTGTGCTGTTTGACCATGATGTGGTGGTGCAAAAGGATCTAAGTAGATTATGGAGTGTTGATATGAAGGGAAAAGTAAATGGAGCAGTTGAAAATTGTCAGGAGAGTGAAACTTCCTTCCTTCGGATGGATACATACATCAACTTCTCAGATCCATTTGTGGCAAAGAAGTTTGATGCCAATGCATGCACGTGGGCATTTGGGATGAATTTGTTTGACCTTCAAGAGTGGAGGAGAAAAGATCTGACTAAACTCTACCACAAATACTTGAAAATGGGTTATAGAAGGCCATTGTGGGTGGCTGGGAGTTTGCCCTTAGGTTGGGTCACCTTCTATAACCAGACGGTAGCTTTGGACAGGCGGTGGCACATCCTTGGGCTGGGTTACGACTCAGATGTGGTTCATGCTGACATTGATCGAGCGGCAGTAATACACTATGATGGAATCATGAAGCCATGGTTAGACATCGCGATTGCGAGGTACAAGGATTATTGGAGAAAATATGTCAACTATGACGACTCTCATCTGCAACAGTGCAACATTCATGCGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000139 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005794 GO:0012505 GO:0016020 GO:0031090 GO:0031984 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0098588 GO:0098791

KEGG Pathways

Metabolic & Signaling

ko00520 map00520

Pfam Domains

Protein Families

Glyco_transf_8

Protein Analysis

625

Amino Acids

71.91

Weight (kDa)

9.29

Isoelectric Point (pI)

45.13

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GAUT_1	PF25557	187 - 259	1.9e-28	Galacturonosyltransferase domain 1
Glyco_transf_8	PF01501	305 - 598	2.1e-61	Glycosyl transferase family 8

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008494)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G06780 AT1G06780 AT2G30575
fragaria_vesca	FvH4_2g22460 FvH4_2g22460
malus_domestica	MD05G1204900.v1.1 MD10G1192000.v1.1
prunus_persica	Prupe.8G231500_v2.0.a1 Prupe.8G231500_v2.0.a1
pyrus_communis	pycom05g19110 pycom10g16590
rosa_chinensis	RchiOBHm_Chr6g0289401
rosa_laevigata	RLG00000012307
rosa_multiflora	Rmu_co8414935.1_g000001
rosa_roxburghii	Rroxscaffold_7G00179240
rosa_rugosa	Rorug06G0203100 Rorug06G0203200 Rorug06G0203300
rosa_samantha	Rh6AG314200 Rh6BG321800 Rh6CG328000 Rh6DG313700
rosa_wichuraiana	Rw6G027160

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB7I	CCANNNNNTGG	1 cut(s)	1345
AccBSI	CCGCTC	1 cut(s)	1745
AccI	GTMKAC	3 cut(s)	907, 1214, 1262
AccII	CGCG	2 cut(s)	65, 1793
AccIII	TCCGGA	1 cut(s)	293
AciI	CCGC	3 cut(s)	115, 1684, 1745
AclWI	GGATC	5 cut(s)	22, 35, 441, 1368, 1469
AcsI	RAATTY	2 cut(s)	541, 1531
AcuI	CTGAAG	2 cut(s)	362, 852
AcvI	CACGTG	1 cut(s)	1515
AfaI	GTAC	4 cut(s)	444, 1045, 1223, 1805
AfiI	CCNNNNNNNGG	7 cut(s)	405, 963, 1345, 1446, 1450, 1644, 1702
AjnI	CCWGG	2 cut(s)	622, 1162
AjuI	GAANNNNNNNTTGG	2 cut(s)	669, 701
AluBI	AGCT	9 cut(s)	7, 172, 201, 382, 596, 808, 928, 1003, 1673
AluI	AGCT	9 cut(s)	7, 172, 201, 382, 596, 808, 928, 1003, 1673
Alw26I	GTCTC	5 cut(s)	58, 82, 85, 330, 868
AlwI	GGATC	5 cut(s)	22, 35, 441, 1368, 1469
Aor13HI	TCCGGA	1 cut(s)	293
AoxI	GGCC	1 cut(s)	1610
ApeKI	GCWGC	2 cut(s)	884, 925
ApoI	RAATTY	2 cut(s)	541, 1531
ArsI	GACNNNNNNTTYG	2 cut(s)	1657, 1689
AseI	ATTAAT	1 cut(s)	276
AspA2I	CCTAGG	1 cut(s)	1256
AspLEI	GCGC	1 cut(s)	67
AsuC2I	CCSGG	1 cut(s)	1310
AsuHPI	GGTGA	2 cut(s)	1120, 1642
AvrII	CCTAGG	1 cut(s)	1256
AxyI	CCTNAGG	1 cut(s)	1639
BamHI	GGATCC	1 cut(s)	27
BbrPI	CACGTG	1 cut(s)	1515
BbsI	GAAGAC	2 cut(s)	67, 744
BbvI	GCAGC	2 cut(s)	871, 937
BccI	CCATC	3 cut(s)	77, 1444, 1757
BcgI	CGANNNNNNTGC	2 cut(s)	512, 546
BciT130I	CCWGG	2 cut(s)	624, 1164
BciVI	GTATCC	1 cut(s)	1447
BcnI	CCSGG	1 cut(s)	1310
BcoDI	GTCTC	5 cut(s)	58, 82, 85, 330, 868
BfaI	CTAG	1 cut(s)	1257
BfmI	CTRYAG	1 cut(s)	933
BfuI	GTATCC	1 cut(s)	1447
BglI	GCCNNNNNGGC	1 cut(s)	112
BglII	AGATCT	1 cut(s)	1564
BisI	GCNGC	3 cut(s)	885, 926, 1746
BlnI	CCTAGG	1 cut(s)	1256
BlpI	GCTNAGC	1 cut(s)	173
BlsI	GCNGC	3 cut(s)	886, 927, 1747
Bme1390I	CCNGG	3 cut(s)	624, 1164, 1310
BmiI	GGNNCC	2 cut(s)	29, 694
BmrFI	CCNGG	3 cut(s)	624, 1164, 1310
BmrI	ACTGGG	1 cut(s)	120
BmsI	GCATC	4 cut(s)	8, 223, 1105, 1489
BmuI	ACTGGG	1 cut(s)	120
BpiI	GAAGAC	2 cut(s)	67, 744
BpmI	CTGGAG	2 cut(s)	354, 1231
Bpu1102I	GCTNAGC	1 cut(s)	173
BpuEI	CTTGAG	1 cut(s)	628
BpuMI	CCSGG	1 cut(s)	1310
BsaAI	YACGTR	1 cut(s)	1515
BsaBI	GATNNNNATC	1 cut(s)	32
BsaJI	CCNNGG	5 cut(s)	699, 957, 1256, 1696, 1778
BsaWI	WCCGGW	2 cut(s)	153, 293
BsaXI	ACNNNNNCTCC	2 cut(s)	953, 983
Bsc4I	CCNNNNNNNGG	7 cut(s)	405, 963, 1345, 1446, 1450, 1644, 1702
Bse1I	ACTGG	3 cut(s)	115, 337, 1248
Bse21I	CCTNAGG	1 cut(s)	1639
Bse8I	GATNNNNATC	1 cut(s)	32
BseAI	TCCGGA	1 cut(s)	293
BseBI	CCWGG	2 cut(s)	624, 1164
BseDI	CCNNGG	5 cut(s)	699, 957, 1256, 1696, 1778
BseGI	GGATG	4 cut(s)	33, 1455, 1533, 1692
BseJI	GATNNNNATC	1 cut(s)	32
BseLI	CCNNNNNNNGG	7 cut(s)	405, 963, 1345, 1446, 1450, 1644, 1702
BseMII	CTCAG	4 cut(s)	374, 464, 1485, 1728
BseNI	ACTGG	3 cut(s)	115, 337, 1248
BseRI	GAGGAG	2 cut(s)	134, 1570
BseXI	GCAGC	2 cut(s)	871, 937
BseYI	CCCAGC	2 cut(s)	1625, 1702
BsgI	GTGCAG	1 cut(s)	531
Bsh1236I	CGCG	2 cut(s)	65, 1793
BshFI	GGCC	1 cut(s)	1612
BsiSI	CCGG	3 cut(s)	154, 294, 1310
BslFI	GGGAC	1 cut(s)	320
BslI	CCNNNNNNNGG	7 cut(s)	405, 963, 1345, 1446, 1450, 1644, 1702
BsmAI	GTCTC	5 cut(s)	58, 82, 85, 330, 868
BsmBI	CGTCTC	3 cut(s)	58, 85, 330
BsmFI	GGGAC	1 cut(s)	320
BsmI	GAATGC	1 cut(s)	535
BsnI	GGCC	1 cut(s)	1612
Bsp13I	TCCGGA	1 cut(s)	293
Bsp1407I	TGTACA	2 cut(s)	442, 1043
Bsp143I	GATC	8 cut(s)	27, 433, 523, 580, 1360, 1474, 1564, 1738
Bsp1720I	GCTNAGC	1 cut(s)	173
Bsp19I	CCATGG	1 cut(s)	1778
Bsp68I	TCGCGA	1 cut(s)	1793
BspACI	CCGC	3 cut(s)	115, 1684, 1745
BspANI	GGCC	1 cut(s)	1612
BspCNI	CTCAG	4 cut(s)	375, 463, 1484, 1727
BspEI	TCCGGA	1 cut(s)	293
BspFNI	CGCG	2 cut(s)	65, 1793
BspHI	TCATGA	1 cut(s)	1770
BspLI	GGNNCC	2 cut(s)	29, 694
BspMAI	CTGCAG	1 cut(s)	937
BspPI	GGATC	5 cut(s)	22, 35, 441, 1368, 1469
BspQI	GCTCTTC	2 cut(s)	796, 828
BsrBI	CCGCTC	1 cut(s)	1745
BsrGI	TGTACA	2 cut(s)	442, 1043
BsrI	ACTGG	3 cut(s)	115, 337, 1248
BssECI	CCNNGG	5 cut(s)	699, 957, 1256, 1696, 1778
BssMI	GATC	8 cut(s)	27, 433, 523, 580, 1360, 1474, 1564, 1738
BssNAI	GTATAC	1 cut(s)	1263
BssT1I	CCWWGG	5 cut(s)	699, 957, 1256, 1696, 1778
Bst1107I	GTATAC	1 cut(s)	1263
Bst2UI	CCWGG	2 cut(s)	624, 1164
Bst4CI	ACNGT	5 cut(s)	253, 1048, 1066, 1669, 1857
Bst6I	CTCTTC	2 cut(s)	796, 828
BstAUI	TGTACA	2 cut(s)	442, 1043
BstBAI	YACGTR	1 cut(s)	1515
BstC8I	GCNNGC	3 cut(s)	769, 889, 1510
BstDEI	CTNAG	7 cut(s)	173, 383, 450, 1364, 1471, 1639, 1714
BstDSI	CCRYGG	1 cut(s)	1778
BstEII	GGTNACC	1 cut(s)	1648
BstF5I	GGATG	4 cut(s)	33, 1455, 1533, 1692
BstFNI	CGCG	2 cut(s)	65, 1793
BstHHI	GCGC	1 cut(s)	67
BstKTI	GATC	8 cut(s)	30, 436, 526, 583, 1363, 1477, 1567, 1741
BstMAI	GTCTC	5 cut(s)	58, 82, 85, 330, 868
BstMBI	GATC	8 cut(s)	27, 433, 523, 580, 1360, 1474, 1564, 1738
BstMWI	GCNNNNNNNGC	4 cut(s)	112, 379, 856, 893
BstNI	CCWGG	2 cut(s)	624, 1164
BstNSI	RCATGY	2 cut(s)	1044, 1512
BstPI	GGTNACC	1 cut(s)	1648
BstSCI	CCNGG	3 cut(s)	622, 1162, 1308
BstSFI	CTRYAG	1 cut(s)	933
BstUI	CGCG	2 cut(s)	65, 1793
BstV1I	GCAGC	2 cut(s)	871, 937
BstV2I	GAAGAC	2 cut(s)	67, 744
BstX2I	RGATCY	4 cut(s)	27, 1360, 1474, 1564
BstYI	RGATCY	4 cut(s)	27, 1360, 1474, 1564
BstZ17I	GTATAC	1 cut(s)	1263
Bsu36I	CCTNAGG	1 cut(s)	1639
BsuI	GTATCC	1 cut(s)	1447
BsuRI	GGCC	1 cut(s)	1612
BtgI	CCRYGG	1 cut(s)	1778
BtgZI	GCGATG	1 cut(s)	1774
BtsCI	GGATG	4 cut(s)	33, 1455, 1533, 1692
BtsI	GCAGTG	1 cut(s)	1071
BtsIMutI	CAGTG	5 cut(s)	1053, 1062, 1071, 1255, 1862
BtuMI	TCGCGA	1 cut(s)	1793
Cac8I	GCNNGC	3 cut(s)	769, 889, 1510
CciI	TCATGA	1 cut(s)	1770
CfoI	GCGC	1 cut(s)	67
CseI	GACGC	3 cut(s)	40, 166, 317
Csp6I	GTAC	4 cut(s)	443, 1044, 1222, 1804
CviQI	GTAC	4 cut(s)	443, 1044, 1222, 1804
DdeI	CTNAG	7 cut(s)	173, 383, 450, 1364, 1471, 1639, 1714
DpnI	GATC	8 cut(s)	29, 435, 525, 582, 1362, 1476, 1566, 1740
DpnII	GATC	8 cut(s)	27, 433, 523, 580, 1360, 1474, 1564, 1738
DraI	TTTAAA	2 cut(s)	540, 546
Eam1104I	CTCTTC	2 cut(s)	796, 828
EarI	CTCTTC	2 cut(s)	796, 828
Eco130I	CCWWGG	5 cut(s)	699, 957, 1256, 1696, 1778
Eco57I	CTGAAG	2 cut(s)	362, 852
Eco72I	CACGTG	1 cut(s)	1515
Eco81I	CCTNAGG	1 cut(s)	1639
Eco91I	GGTNACC	1 cut(s)	1648
EcoO65I	GGTNACC	1 cut(s)	1648
EcoRII	CCWGG	2 cut(s)	622, 1162
EcoT14I	CCWWGG	5 cut(s)	699, 957, 1256, 1696, 1778
EcoT22I	ATGCAT	1 cut(s)	1510
ErhI	CCWWGG	5 cut(s)	699, 957, 1256, 1696, 1778
Esp3I	CGTCTC	3 cut(s)	58, 85, 330
FaqI	GGGAC	1 cut(s)	320
FauI	CCCGC	1 cut(s)	108
FblI	GTMKAC	3 cut(s)	907, 1214, 1262
Fnu4HI	GCNGC	3 cut(s)	885, 926, 1746
FokI	GGATG	4 cut(s)	20, 1462, 1540, 1679
Fsp4HI	GCNGC	3 cut(s)	885, 926, 1746
FspBI	CTAG	1 cut(s)	1257
GlaI	GCGC	1 cut(s)	66
GluI	GCNGC	3 cut(s)	885, 926, 1746
GsaI	CCCAGC	2 cut(s)	1629, 1706
GsuI	CTGGAG	2 cut(s)	354, 1231
HaeIII	GGCC	1 cut(s)	1612
HapII	CCGG	3 cut(s)	154, 294, 1310
HgaI	GACGC	3 cut(s)	40, 166, 317
HhaI	GCGC	1 cut(s)	67
Hin6I	GCGC	1 cut(s)	65
HinP1I	GCGC	1 cut(s)	65
HincII	GTYRAC	2 cut(s)	1215, 1831
HindII	GTYRAC	2 cut(s)	1215, 1831
HindIII	AAGCTT	2 cut(s)	170, 199
HpaII	CCGG	3 cut(s)	154, 294, 1310
HphI	GGTGA	2 cut(s)	1120, 1642
Hpy166II	GTNNAC	7 cut(s)	445, 801, 908, 1057, 1215, 1263, 1831
Hpy188I	TCNGA	8 cut(s)	438, 528, 709, 1270, 1449, 1474, 1569, 1717
Hpy188III	TCNNGA	7 cut(s)	294, 950, 1186, 1424, 1547, 1771, 1792
Hpy8I	GTNNAC	7 cut(s)	445, 801, 908, 1057, 1215, 1263, 1831
Hpy99I	CGWCG	2 cut(s)	18, 182
HpyAV	CCTTC	7 cut(s)	640, 1384, 1450, 1454, 1553, 1602, 1663
HpyCH4III	ACNGT	5 cut(s)	253, 1048, 1066, 1669, 1857
HpyCH4IV	ACGT	2 cut(s)	495, 1514
HpyF10VI	GCNNNNNNNGC	4 cut(s)	112, 379, 856, 893
HpyF3I	CTNAG	7 cut(s)	173, 383, 450, 1364, 1471, 1639, 1714
HpySE526I	ACGT	2 cut(s)	495, 1514
HspAI	GCGC	1 cut(s)	65
Kpn2I	TCCGGA	1 cut(s)	293
Kzo9I	GATC	8 cut(s)	27, 433, 523, 580, 1360, 1474, 1564, 1738
LguI	GCTCTTC	2 cut(s)	796, 828
LmnI	GCTCC	4 cut(s)	354, 692, 813, 1406
Lsp1109I	GCAGC	2 cut(s)	871, 937
LweI	GCATC	4 cut(s)	8, 223, 1105, 1489
MaeI	CTAG	1 cut(s)	1257
MaeII	ACGT	2 cut(s)	495, 1514
MaeIII	GTNAC	5 cut(s)	553, 1108, 1250, 1648, 1706
MalI	GATC	8 cut(s)	29, 435, 525, 582, 1362, 1476, 1566, 1740
MbiI	CCGCTC	1 cut(s)	1745
MboI	GATC	8 cut(s)	27, 433, 523, 580, 1360, 1474, 1564, 1738
MboII	GAAGA	9 cut(s)	67, 242, 574, 744, 783, 845, 865, 1102, 1297
MfeI	CAATTG	2 cut(s)	419, 684
MflI	RGATCY	4 cut(s)	27, 1360, 1474, 1564
MluCI	AATT	8 cut(s)	256, 419, 541, 663, 684, 1123, 1417, 1531
MlyI	GAGTC	4 cut(s)	758, 814, 1706, 1835
MmeI	TCCRAC	2 cut(s)	303, 1050
Mph1103I	ATGCAT	1 cut(s)	1510
MroI	TCCGGA	1 cut(s)	293
MseI	TTAA	8 cut(s)	147, 255, 276, 395, 539, 545, 666, 1154
MslI	CAYNNNNRTG	2 cut(s)	1513, 1761
MspA1I	CMGCKG	2 cut(s)	382, 928
MspI	CCGG	3 cut(s)	154, 294, 1310
MspR9I	CCNGG	3 cut(s)	624, 1164, 1310
MunI	CAATTG	2 cut(s)	419, 684
Mva1269I	GAATGC	1 cut(s)	535
MvaI	CCWGG	2 cut(s)	624, 1164
MvnI	CGCG	2 cut(s)	65, 1793
MwoI	GCNNNNNNNGC	4 cut(s)	112, 379, 856, 893
NciI	CCSGG	1 cut(s)	1310
NcoI	CCATGG	1 cut(s)	1778
NdeII	GATC	8 cut(s)	27, 433, 523, 580, 1360, 1474, 1564, 1738
NlaIV	GGNNCC	2 cut(s)	29, 694
NmeAIII	GCCGAG	1 cut(s)	237
NmuCI	GTSAC	3 cut(s)	1108, 1250, 1648
NruI	TCGCGA	1 cut(s)	1793
NsiI	ATGCAT	1 cut(s)	1510
NspI	RCATGY	2 cut(s)	1044, 1512
PaeI	GCATGC	1 cut(s)	1512
PagI	TCATGA	1 cut(s)	1770
PciSI	GCTCTTC	2 cut(s)	796, 828
PcsI	WCGNNNNNNNCGW	1 cut(s)	69
PctI	GAATGC	1 cut(s)	535
PfeI	GAWTC	7 cut(s)	188, 290, 342, 704, 953, 995, 1767
PflMI	CCANNNNNTGG	1 cut(s)	1345
PfoI	TCCNGGA	1 cut(s)	1162
PkrI	GCNGC	3 cut(s)	886, 927, 1747
PleI	GAGTC	4 cut(s)	757, 814, 1706, 1835
PmaCI	CACGTG	1 cut(s)	1515
PmlI	CACGTG	1 cut(s)	1515
PpsI	GAGTC	4 cut(s)	757, 814, 1706, 1835
Ppu21I	YACGTR	1 cut(s)	1515
PshBI	ATTAAT	1 cut(s)	276
Psp6I	CCWGG	2 cut(s)	622, 1162
PspCI	CACGTG	1 cut(s)	1515
PspEI	GGTNACC	1 cut(s)	1648
PspFI	CCCAGC	2 cut(s)	1625, 1702
PspGI	CCWGG	2 cut(s)	622, 1162
PspN4I	GGNNCC	2 cut(s)	29, 694
PstI	CTGCAG	1 cut(s)	937
PsuI	RGATCY	4 cut(s)	27, 1360, 1474, 1564
PvuII	CAGCTG	2 cut(s)	382, 928
RruI	TCGCGA	1 cut(s)	1793
RsaI	GTAC	4 cut(s)	444, 1045, 1223, 1805
RsaNI	GTAC	4 cut(s)	443, 1044, 1222, 1804
RseI	CAYNNNNRTG	2 cut(s)	1513, 1761
SalI	GTCGAC	1 cut(s)	1213
SapI	GCTCTTC	2 cut(s)	796, 828
SaqAI	TTAA	8 cut(s)	147, 255, 276, 395, 539, 545, 666, 1154
SatI	GCNGC	3 cut(s)	885, 926, 1746
Sau3AI	GATC	8 cut(s)	27, 433, 523, 580, 1360, 1474, 1564, 1738
SchI	GAGTC	4 cut(s)	758, 814, 1706, 1835
ScrFI	CCNGG	3 cut(s)	624, 1164, 1310
SfaNI	GCATC	4 cut(s)	8, 223, 1105, 1489
SfcI	CTRYAG	1 cut(s)	933
SmiI	ATTTAAAT	1 cut(s)	540
SmiMI	CAYNNNNRTG	2 cut(s)	1513, 1761
SmlI	CTYRAG	1 cut(s)	607
SmoI	CTYRAG	1 cut(s)	607
SphI	GCATGC	1 cut(s)	1512
Sse9I	AATT	8 cut(s)	256, 419, 541, 663, 684, 1123, 1417, 1531
SsiI	CCGC	3 cut(s)	115, 1684, 1745
SspI	AATATT	2 cut(s)	145, 393
SspMI	CTAG	1 cut(s)	1257
StyD4I	CCNGG	3 cut(s)	622, 1162, 1308
StyI	CCWWGG	5 cut(s)	699, 957, 1256, 1696, 1778
SwaI	ATTTAAAT	1 cut(s)	540
TaaI	ACNGT	5 cut(s)	253, 1048, 1066, 1669, 1857
TaiI	ACGT	2 cut(s)	498, 1517
TaqI	TCGA	6 cut(s)	16, 140, 180, 191, 1214, 1741
TasI	AATT	8 cut(s)	256, 419, 541, 663, 684, 1123, 1417, 1531
TatI	WGTACW	3 cut(s)	442, 1043, 1221
TauI	GCSGC	1 cut(s)	1748
TfiI	GAWTC	7 cut(s)	188, 290, 342, 704, 953, 995, 1767
Tru1I	TTAA	8 cut(s)	147, 255, 276, 395, 539, 545, 666, 1154
Tru9I	TTAA	8 cut(s)	147, 255, 276, 395, 539, 545, 666, 1154
TscAI	CASTG	5 cut(s)	1053, 1069, 1078, 1255, 1862
TseFI	GTSAC	3 cut(s)	1108, 1250, 1648
TseI	GCWGC	2 cut(s)	884, 925
Tsp45I	GTSAC	3 cut(s)	1108, 1250, 1648
TspDTI	ATGAA	9 cut(s)	17, 115, 503, 590, 1403, 1544, 1715, 1787, 1856
TspGWI	ACGGA	3 cut(s)	354, 800, 1274
TspRI	CASTG	5 cut(s)	1053, 1069, 1078, 1255, 1862
Van91I	CCANNNNNTGG	1 cut(s)	1345
VspI	ATTAAT	1 cut(s)	276
XapI	RAATTY	2 cut(s)	541, 1531
XceI	RCATGY	2 cut(s)	1044, 1512
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1033
XmaJI	CCTAGG	1 cut(s)	1256
XmiI	GTMKAC	3 cut(s)	907, 1214, 1262
XspI	CTAG	1 cut(s)	1257
Zsp2I	ATGCAT	1 cut(s)	1510

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.