Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0014936

Physical Location & Seq

Genomic Coordinates Reverse (-)

35364371 .. 35366978

Gene Structure 2608 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1221100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 981 bp

ATGGATAACACATATTATAGTCCTAGCACTAAGGATGATCACCAGTATACTGATGATGATGAAGATGATGTTCAACTACCAGGATTTCGGTTTCACCCAACAGATGAAGAACTTGTAGACTTCTATCTTCGTCGGAAGATTCAGAAGAAACCGATCAGCTTCGAGCTCATCAAATCAATTGATATCTACAAATATGATCCTTGGGATCTTCCAAAAACCGCAGGAGATAAGGAATGGTACTTCTTCTGCAAAAGAGGGAGAAAGTACAAGAACAGCTTCAGACCCAACAGAGTAACAGGTTCTGGATTTTGGAAGGCAACTGGAATCGACAAGCCGATACATTCCCACGGAGGAGAGGGCCATGCCTGCAATGGCCTAAAGAAAACATTGGTTTACTACCGTGGAAGTGCAGGCAAAGGCAGCAAAACCGATTGGATGATGCACGAGTTTCGCCTTCCTAATTCCTCCAGTTCGAATGAATACAATAACCGAAGCTCTAACCCTAAGAATATGAATACTGATCCTCAACAAGAAGCTGAAATATGGACATTGTGTCGGATATTCAAAAGAAACGTTTCATACAGAAAGTACACCCCAGATTGGAGAGATCTATCTGCAGCCAAACGTCATCCAACGGACACCGTAAACTCCAAGAAATTCGCTACTGATCGACAAGACCTTCTCGACCAATCTAACACTTATATCAGTTTCGGTGCTTCAGACATTTGCTACGAGGAGAAGCCTCTTGTGAATCATACCAACAATATAAACAGTACTAATGGAAGTCACCAATTGCATGCGCATGCAGTTGGGCAGTTGAGCTCCAACATGCAGCAGCCTAATTCAACACATCCATACATGGACACGTTCTCAAACTTTTCGTTTCAAGATCTTGAGAATGATCTTTTGAATGAAAATTGGGATGAGCTTAGATCAGTCGTACAACAGCTTGCTTTTGATCCGACGCCATTTCCTGTATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003700 GO:0005975 GO:0005984 GO:0005991 GO:0005992 GO:0006082 GO:0006355 GO:0006520 GO:0006560 GO:0006561 GO:0006725 GO:0006790 GO:0006807 GO:0006950 GO:0006970 GO:0006972 GO:0007275 GO:0007568 GO:0008150 GO:0008152 GO:0008652 GO:0009058 GO:0009064 GO:0009084 GO:0009311 GO:0009312 GO:0009403 GO:0009404 GO:0009628 GO:0009651 GO:0009700 GO:0009718 GO:0009812 GO:0009813 GO:0009889 GO:0009987 GO:0010120 GO:0010150 GO:0010468 GO:0010556 GO:0016051 GO:0016053 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0019748 GO:0019752 GO:0031323 GO:0031326 GO:0032501 GO:0032502 GO:0034637 GO:0034641 GO:0042430 GO:0042435 GO:0042440 GO:0042538 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044262 GO:0044271 GO:0044272 GO:0044281 GO:0044283 GO:0044550 GO:0046148 GO:0046217 GO:0046283 GO:0046351 GO:0046394 GO:0046483 GO:0048366 GO:0048367 GO:0048519 GO:0048731 GO:0048827 GO:0048831 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051093 GO:0051171 GO:0051239 GO:0051241 GO:0051252 GO:0052314 GO:0052315 GO:0052317 GO:0060255 GO:0065007 GO:0071704 GO:0080090 GO:0090693 GO:0099402 GO:0140110 GO:1900055 GO:1900056 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576 GO:1901605 GO:1901607 GO:1903506 GO:1905622 GO:2000024 GO:2000026 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

NAM

Protein Analysis

327

Amino Acids

37.9

Weight (kDa)

6.46

Isoelectric Point (pI)

33.45

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
NAM	PF02365	27 - 152	7.6e-46	No apical meristem (NAM) protein

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Orthologous Genes (Group: OG0014936)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G12910
fragaria_vesca	FvH4_3g15610
malus_domestica	MD00G1130200.v1.1 MD05G1221100.v1.1
prunus_persica	Prupe.4G138500_v2.0.a1
pyrus_communis	pycom05g20440 pycom10g17400
rosa_chinensis	RchiOBHm_Chr5g0026231
rosa_laevigata	RLG00000032915
rosa_multiflora	Rmu_sc0000632.1_g000004
rosa_roxburghii	Rroxscaffold_1G00053080
rosa_rugosa	Rorug05G0091600
rosa_samantha	Rh5AG184700 Rh5CG200800 Rh5DG183300
rosa_wichuraiana	Rw5G016750

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	801
AccI	GTMKAC	2 cut(s)	47, 117
AciI	CCGC	1 cut(s)	219
AclI	AACGTT	1 cut(s)	573
AclWI	GGATC	4 cut(s)	191, 213, 515, 953
AcsI	RAATTY	1 cut(s)	656
AcuI	CTGAAG	2 cut(s)	262, 702
AcyI	GRCGYC	1 cut(s)	965
AfaI	GTAC	5 cut(s)	239, 266, 590, 775, 942
AfiI	CCNNNNNNNGG	1 cut(s)	600
AflIII	ACRYGT	1 cut(s)	864
AgsI	TTSAA	5 cut(s)	74, 565, 846, 887, 910
AhdI	GACNNNNNGTC	1 cut(s)	552
AjnI	CCWGG	1 cut(s)	79
AluBI	AGCT	8 cut(s)	159, 166, 276, 495, 536, 822, 928, 949
AluI	AGCT	8 cut(s)	159, 166, 276, 495, 536, 822, 928, 949
Alw21I	GWGCWC	2 cut(s)	168, 824
AlwI	GGATC	4 cut(s)	191, 213, 515, 953
AlwNI	CAGNNNCTG	1 cut(s)	302
AoxI	GGCC	2 cut(s)	358, 373
ApeKI	GCWGC	4 cut(s)	420, 617, 832, 835
ApoI	RAATTY	1 cut(s)	656
Asp700I	GAANNNNTTC	2 cut(s)	275, 574
AspLEI	GCGC	1 cut(s)	802
AspS9I	GGNCC	1 cut(s)	358
AsuHPI	GGTGA	3 cut(s)	32, 86, 779
AsuII	TTCGAA	1 cut(s)	473
BanII	GRGCYC	2 cut(s)	168, 824
BauI	CACGAG	1 cut(s)	443
Bbv12I	GWGCWC	2 cut(s)	168, 824
BbvI	GCAGC	4 cut(s)	432, 629, 844, 847
BcgI	CGANNNNNNTGC	2 cut(s)	431, 465
BciT130I	CCWGG	1 cut(s)	81
BclI	TGATCA	1 cut(s)	37
BfaI	CTAG	1 cut(s)	24
BfmI	CTRYAG	1 cut(s)	615
BglII	AGATCT	2 cut(s)	607, 889
BisI	GCNGC	4 cut(s)	421, 618, 833, 836
BlsI	GCNGC	4 cut(s)	422, 619, 834, 837
BmcAI	AGTACT	1 cut(s)	775
Bme1390I	CCNGG	1 cut(s)	81
BmeRI	GACNNNNNGTC	1 cut(s)	552
BmgT120I	GGNCC	1 cut(s)	358
BmrFI	CCNGG	1 cut(s)	81
BmsI	GCATC	1 cut(s)	429
BpmI	CTGGAG	1 cut(s)	451
Bpu14I	TTCGAA	1 cut(s)	473
BpuEI	CTTGAG	1 cut(s)	914
BsaHI	GRCGYC	1 cut(s)	965
BsaJI	CCNNGG	3 cut(s)	200, 346, 400
Bsc4I	CCNNNNNNNGG	1 cut(s)	600
Bse1I	ACTGG	3 cut(s)	43, 325, 468
Bse3DI	GCAATG	1 cut(s)	376
BseBI	CCWGG	1 cut(s)	81
BseDI	CCNNGG	3 cut(s)	200, 346, 400
BseGI	GGATG	5 cut(s)	40, 441, 628, 850, 928
BseLI	CCNNNNNNNGG	1 cut(s)	600
BseMI	GCAATG	1 cut(s)	376
BseNI	ACTGG	3 cut(s)	43, 325, 468
BseRI	GAGGAG	2 cut(s)	366, 749
BseXI	GCAGC	4 cut(s)	432, 629, 844, 847
BsgI	GTGCAG	1 cut(s)	429
BshFI	GGCC	2 cut(s)	360, 375
BsiHKAI	GWGCWC	2 cut(s)	168, 824
BslI	CCNNNNNNNGG	1 cut(s)	600
BsnI	GGCC	2 cut(s)	360, 375
Bsp119I	TTCGAA	1 cut(s)	473
Bsp1286I	GDGCHC	2 cut(s)	168, 824
BspACI	CCGC	1 cut(s)	219
BspANI	GGCC	2 cut(s)	360, 375
BspMAI	CTGCAG	1 cut(s)	619
BspPI	GGATC	4 cut(s)	191, 213, 515, 953
BspT104I	TTCGAA	1 cut(s)	473
BsrDI	GCAATG	1 cut(s)	376
BsrI	ACTGG	3 cut(s)	43, 325, 468
BssECI	CCNNGG	3 cut(s)	200, 346, 400
BssNAI	GTATAC	1 cut(s)	48
BssNI	GRCGYC	1 cut(s)	965
BssSI	CACGAG	1 cut(s)	443
BssT1I	CCWWGG	1 cut(s)	200
Bst1107I	GTATAC	1 cut(s)	48
Bst2BI	CACGAG	1 cut(s)	443
Bst2UI	CCWGG	1 cut(s)	81
Bst4CI	ACNGT	3 cut(s)	401, 643, 773
BstACI	GRCGYC	1 cut(s)	965
BstBI	TTCGAA	1 cut(s)	473
BstC8I	GCNNGC	5 cut(s)	367, 412, 798, 804, 951
BstDEI	CTNAG	3 cut(s)	30, 504, 929
BstDSI	CCRYGG	2 cut(s)	346, 400
BstF5I	GGATG	5 cut(s)	40, 441, 628, 850, 928
BstHHI	GCGC	1 cut(s)	802
BstMWI	GCNNNNNNNGC	2 cut(s)	366, 420
BstNI	CCWGG	1 cut(s)	81
BstNSI	RCATGY	3 cut(s)	800, 806, 832
BstSCI	CCNGG	1 cut(s)	79
BstSFI	CTRYAG	1 cut(s)	615
BstV1I	GCAGC	4 cut(s)	432, 629, 844, 847
BstX2I	RGATCY	3 cut(s)	205, 607, 889
BstYI	RGATCY	3 cut(s)	205, 607, 889
BstZ17I	GTATAC	1 cut(s)	48
BsuRI	GGCC	2 cut(s)	360, 375
BtgI	CCRYGG	2 cut(s)	346, 400
BtsCI	GGATG	5 cut(s)	40, 441, 628, 850, 928
Cac8I	GCNNGC	5 cut(s)	367, 412, 798, 804, 951
CaiI	CAGNNNCTG	1 cut(s)	302
CfoI	GCGC	1 cut(s)	802
Cfr13I	GGNCC	1 cut(s)	358
CseI	GACGC	1 cut(s)	973
Csp6I	GTAC	5 cut(s)	238, 265, 589, 774, 941
CviAII	CATG	5 cut(s)	362, 797, 803, 829, 859
CviQI	GTAC	5 cut(s)	238, 265, 589, 774, 941
DdeI	CTNAG	3 cut(s)	30, 504, 929
DriI	GACNNNNNGTC	1 cut(s)	552
Eam1105I	GACNNNNNGTC	1 cut(s)	552
Ecl136II	GAGCTC	2 cut(s)	166, 822
Eco130I	CCWWGG	1 cut(s)	200
Eco24I	GRGCYC	2 cut(s)	168, 824
Eco32I	GATATC	1 cut(s)	184
Eco53kI	GAGCTC	2 cut(s)	166, 822
Eco57I	CTGAAG	2 cut(s)	262, 702
EcoICRI	GAGCTC	2 cut(s)	166, 822
EcoRII	CCWGG	1 cut(s)	79
EcoRV	GATATC	1 cut(s)	184
EcoT14I	CCWWGG	1 cut(s)	200
EcoT38I	GRGCYC	2 cut(s)	168, 824
ErhI	CCWWGG	1 cut(s)	200
FaeI	CATG	5 cut(s)	365, 800, 806, 832, 862
FalI	AAGNNNNNCTT	2 cut(s)	260, 292
FatI	CATG	5 cut(s)	361, 796, 802, 828, 858
FbaI	TGATCA	1 cut(s)	37
FblI	GTMKAC	2 cut(s)	47, 117
Fnu4HI	GCNGC	4 cut(s)	421, 618, 833, 836
FokI	GGATG	5 cut(s)	47, 448, 615, 837, 935
FriOI	GRGCYC	2 cut(s)	168, 824
Fsp4HI	GCNGC	4 cut(s)	421, 618, 833, 836
FspAI	RTGCGCAY	1 cut(s)	801
FspBI	CTAG	1 cut(s)	24
FspI	TGCGCA	1 cut(s)	801
GlaI	GCGC	1 cut(s)	801
GluI	GCNGC	4 cut(s)	421, 618, 833, 836
GsuI	CTGGAG	1 cut(s)	451
HaeIII	GGCC	2 cut(s)	360, 375
HgaI	GACGC	1 cut(s)	973
HhaI	GCGC	1 cut(s)	802
Hin1I	GRCGYC	1 cut(s)	965
Hin1II	CATG	5 cut(s)	365, 800, 806, 832, 862
Hin6I	GCGC	1 cut(s)	800
HinP1I	GCGC	1 cut(s)	800
HinfI	GANTC	3 cut(s)	139, 324, 751
HphI	GGTGA	3 cut(s)	32, 86, 779
Hpy166II	GTNNAC	5 cut(s)	48, 118, 394, 591, 646
Hpy188I	TCNGA	6 cut(s)	135, 144, 281, 558, 721, 963
Hpy188III	TCNNGA	4 cut(s)	303, 683, 887, 893
Hpy8I	GTNNAC	5 cut(s)	48, 118, 394, 591, 646
Hpy99I	CGWCG	2 cut(s)	135, 967
HpyAV	CCTTC	3 cut(s)	307, 464, 689
HpyCH4III	ACNGT	3 cut(s)	401, 643, 773
HpyCH4IV	ACGT	3 cut(s)	573, 625, 866
HpyCH4V	TGCA	8 cut(s)	249, 369, 410, 442, 617, 796, 806, 832
HpyF10VI	GCNNNNNNNGC	2 cut(s)	366, 420
HpyF3I	CTNAG	3 cut(s)	30, 504, 929
HpySE526I	ACGT	3 cut(s)	573, 625, 866
Hsp92I	GRCGYC	1 cut(s)	965
Hsp92II	CATG	5 cut(s)	365, 800, 806, 832, 862
HspAI	GCGC	1 cut(s)	800
Ksp22I	TGATCA	1 cut(s)	37
LmnI	GCTCC	1 cut(s)	827
Lsp1109I	GCAGC	4 cut(s)	432, 629, 844, 847
LweI	GCATC	1 cut(s)	429
MaeI	CTAG	1 cut(s)	24
MaeII	ACGT	3 cut(s)	573, 625, 866
MaeIII	GTNAC	2 cut(s)	292, 785
MboII	GAAGA	7 cut(s)	74, 119, 119, 148, 157, 200, 235
MfeI	CAATTG	2 cut(s)	177, 791
MflI	RGATCY	3 cut(s)	205, 607, 889
MhlI	GDGCHC	2 cut(s)	168, 824
MluCI	AATT	6 cut(s)	177, 460, 656, 791, 841, 916
MmeI	TCCRAC	4 cut(s)	113, 536, 656, 849
MnlI	CCTC	7 cut(s)	248, 344, 349, 475, 534, 727, 753
MroXI	GAANNNNTTC	2 cut(s)	275, 574
MslI	CAYNNNNRTG	1 cut(s)	801
MspR9I	CCNGG	1 cut(s)	81
MunI	CAATTG	2 cut(s)	177, 791
MvaI	CCWGG	1 cut(s)	81
MwoI	GCNNNNNNNGC	2 cut(s)	366, 420
NlaIII	CATG	5 cut(s)	365, 800, 806, 832, 862
NmuCI	GTSAC	1 cut(s)	785
NsbI	TGCGCA	1 cut(s)	801
NspI	RCATGY	3 cut(s)	800, 806, 832
NspV	TTCGAA	1 cut(s)	473
PaeI	GCATGC	2 cut(s)	800, 806
PdmI	GAANNNNTTC	2 cut(s)	275, 574
PfeI	GAWTC	3 cut(s)	139, 324, 751
PkrI	GCNGC	4 cut(s)	422, 619, 834, 837
Psp124BI	GAGCTC	2 cut(s)	168, 824
Psp1406I	AACGTT	1 cut(s)	573
Psp6I	CCWGG	1 cut(s)	79
PspGI	CCWGG	1 cut(s)	79
PspPI	GGNCC	1 cut(s)	358
PstI	CTGCAG	1 cut(s)	619
PstNI	CAGNNNCTG	1 cut(s)	302
PsuI	RGATCY	3 cut(s)	205, 607, 889
RsaI	GTAC	5 cut(s)	239, 266, 590, 775, 942
RsaNI	GTAC	5 cut(s)	238, 265, 589, 774, 941
RseI	CAYNNNNRTG	1 cut(s)	801
SacI	GAGCTC	2 cut(s)	168, 824
SatI	GCNGC	4 cut(s)	421, 618, 833, 836
Sau96I	GGNCC	1 cut(s)	358
ScaI	AGTACT	1 cut(s)	775
ScrFI	CCNGG	1 cut(s)	81
SduI	GDGCHC	2 cut(s)	168, 824
SfaNI	GCATC	1 cut(s)	429
SfcI	CTRYAG	1 cut(s)	615
SfuI	TTCGAA	1 cut(s)	473
SmiMI	CAYNNNNRTG	1 cut(s)	801
SmlI	CTYRAG	1 cut(s)	893
SmoI	CTYRAG	1 cut(s)	893
SphI	GCATGC	2 cut(s)	800, 806
Sse9I	AATT	6 cut(s)	177, 460, 656, 791, 841, 916
SsiI	CCGC	1 cut(s)	219
SspMI	CTAG	1 cut(s)	24
SstI	GAGCTC	2 cut(s)	168, 824
StyD4I	CCNGG	1 cut(s)	79
StyI	CCWWGG	1 cut(s)	200
TaaI	ACNGT	3 cut(s)	401, 643, 773
TaiI	ACGT	3 cut(s)	576, 628, 869
TaqI	TCGA	5 cut(s)	162, 327, 473, 670, 684
TasI	AATT	6 cut(s)	177, 460, 656, 791, 841, 916
TatI	WGTACW	3 cut(s)	264, 588, 773
TfiI	GAWTC	3 cut(s)	139, 324, 751
TseFI	GTSAC	1 cut(s)	785
TseI	GCWGC	4 cut(s)	420, 617, 832, 835
Tsp45I	GTSAC	1 cut(s)	785
TspDTI	ATGAA	6 cut(s)	75, 120, 492, 527, 567, 927
TspGWI	ACGGA	2 cut(s)	363, 650
XapI	RAATTY	1 cut(s)	656
XceI	RCATGY	3 cut(s)	800, 806, 832
XcmI	CCANNNNNNNNNTGG	1 cut(s)	368
XmiI	GTMKAC	2 cut(s)	47, 117
XmnI	GAANNNNTTC	2 cut(s)	275, 574
XspI	CTAG	1 cut(s)	24
ZrmI	AGTACT	1 cut(s)	775

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.