MD05G1232900.v1.1

Serine threonine-protein phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0016414
Physical Location & Seq
Forward (+)
36572845 .. 36576146
3302 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1232900.v1.1.491

Sequence Viewer

Length: 432 bp
ATGGATTCGGTCCCTTCGAACTCACATGCGAACCTGGATGAACAGATTGCTCAGCTCATGCAGTGCAAGTCCTTGTCTGAACAAGAGGTTTCTTGGGTAGCTGTGGAACATCAGAATACATCACTCTCAAACACCCACTCTTTGGAACCGACCTTCTATGCTGGGTTTCTCAAATGTCTCTCTTATTTTTTAAGGGTGCTCTGCGAGAAGACCAAGGAAATATTAATGGAAGAAAGCAATGTCCAGCCTGTGAAAAGCCCTGTAACTATCTGTGGTGATATTCATGGACAATTCCATGATCTGGCGGAGCTTTTCCGCATTGGAGGAAAGTGCCCAGATACAAACTATTTGTTTATGGGAGATTATGTGGACCGTGGCTATTATTCAGTTGAAACAGTGACATCAAATGTGGGGGCTGCTCTCTTCGTCTAA

Protein Analysis

144

Amino Acids

16.0

Weight (kDa)

4.82

Isoelectric Point (pI)

31.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Metallophos PF00149 87 - 132 3.6e-10 Calcineurin-like phosphoesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 142, 301
AciI CCGC 2 cut(s) 305, 316
AfiI CCNNNNNNNGG 2 cut(s) 142, 301
AgsI TTSAA 1 cut(s) 392
AjnI CCWGG 1 cut(s) 33
AluBI AGCT 3 cut(s) 55, 101, 310
AluI AGCT 3 cut(s) 55, 101, 310
Alw21I GWGCWC 1 cut(s) 201
Alw26I GTCTC 1 cut(s) 182
ApeKI GCWGC 1 cut(s) 416
AseI ATTAAT 1 cut(s) 224
AspS9I GGNCC 2 cut(s) 10, 370
AsuHPI GGTGA 1 cut(s) 287
AsuII TTCGAA 1 cut(s) 17
AvaII GGWCC 2 cut(s) 10, 370
BaeGI GKGCMC 1 cut(s) 335
BbsI GAAGAC 1 cut(s) 215
Bbv12I GWGCWC 1 cut(s) 201
BbvI GCAGC 1 cut(s) 403
BciT130I CCWGG 1 cut(s) 35
BcoDI GTCTC 1 cut(s) 182
BisI GCNGC 1 cut(s) 417
BlpI GCTNAGC 1 cut(s) 51
BlsI GCNGC 1 cut(s) 418
Bme1390I CCNGG 1 cut(s) 35
Bme18I GGWCC 2 cut(s) 10, 370
BmgT120I GGNCC 2 cut(s) 10, 370
BmiI GGNNCC 2 cut(s) 12, 147
BmrFI CCNGG 1 cut(s) 35
BpiI GAAGAC 1 cut(s) 215
Bpu1102I GCTNAGC 1 cut(s) 51
Bpu14I TTCGAA 1 cut(s) 17
BsaJI CCNNGG 2 cut(s) 213, 373
BsaXI ACNNNNNCTCC 2 cut(s) 351, 381
Bsc4I CCNNNNNNNGG 2 cut(s) 142, 301
Bse3DI GCAATG 1 cut(s) 244
BseBI CCWGG 1 cut(s) 35
BseDI CCNNGG 2 cut(s) 213, 373
BseGI GGATG 1 cut(s) 43
BseLI CCNNNNNNNGG 2 cut(s) 142, 301
BseMI GCAATG 1 cut(s) 244
BseMII CTCAG 1 cut(s) 65
BseSI GKGCMC 1 cut(s) 335
BseXI GCAGC 1 cut(s) 403
BseYI CCCAGC 1 cut(s) 161
BsiHKAI GWGCWC 1 cut(s) 201
BslI CCNNNNNNNGG 2 cut(s) 142, 301
BsmAI GTCTC 1 cut(s) 182
Bsp119I TTCGAA 1 cut(s) 17
Bsp1286I GDGCHC 2 cut(s) 201, 335
Bsp143I GATC 1 cut(s) 298
Bsp1720I GCTNAGC 1 cut(s) 51
BspACI CCGC 2 cut(s) 305, 316
BspCNI CTCAG 1 cut(s) 64
BspLI GGNNCC 2 cut(s) 12, 147
BspT104I TTCGAA 1 cut(s) 17
BsrDI GCAATG 1 cut(s) 244
BssECI CCNNGG 2 cut(s) 213, 373
BssMI GATC 1 cut(s) 298
BssT1I CCWWGG 1 cut(s) 213
Bst2UI CCWGG 1 cut(s) 35
Bst4CI ACNGT 2 cut(s) 374, 397
Bst6I CTCTTC 1 cut(s) 428
BstBI TTCGAA 1 cut(s) 17
BstDEI CTNAG 1 cut(s) 51
BstDSI CCRYGG 1 cut(s) 373
BstF5I GGATG 1 cut(s) 43
BstKTI GATC 1 cut(s) 301
BstMAI GTCTC 1 cut(s) 182
BstMBI GATC 1 cut(s) 298
BstNI CCWGG 1 cut(s) 35
BstNSI RCATGY 1 cut(s) 29
BstSCI CCNGG 1 cut(s) 33
BstSLI GKGCMC 1 cut(s) 335
BstV1I GCAGC 1 cut(s) 403
BstV2I GAAGAC 1 cut(s) 215
BtgI CCRYGG 1 cut(s) 373
BtsCI GGATG 1 cut(s) 43
BtsI GCAGTG 1 cut(s) 68
BtsIMutI CAGTG 2 cut(s) 68, 402
Cfr13I GGNCC 2 cut(s) 10, 370
CviAII CATG 4 cut(s) 26, 58, 284, 296
CviJI RGCY 7 cut(s) 55, 101, 247, 258, 310, 378, 416
CviKI_1 RGCY 7 cut(s) 55, 101, 247, 258, 310, 378, 416
DdeI CTNAG 1 cut(s) 51
DpnI GATC 1 cut(s) 300
DpnII GATC 1 cut(s) 298
Eam1104I CTCTTC 1 cut(s) 428
EarI CTCTTC 1 cut(s) 428
EciI GGCGGA 1 cut(s) 320
Eco130I CCWWGG 1 cut(s) 213
Eco47I GGWCC 2 cut(s) 10, 370
EcoRII CCWGG 1 cut(s) 33
EcoT14I CCWWGG 1 cut(s) 213
ErhI CCWWGG 1 cut(s) 213
FaeI CATG 4 cut(s) 29, 61, 287, 299
FaiI YATR 7 cut(s) 27, 59, 159, 285, 297, 356, 366
FatI CATG 4 cut(s) 25, 57, 283, 295
Fnu4HI GCNGC 1 cut(s) 417
FokI GGATG 1 cut(s) 50
Fsp4HI GCNGC 1 cut(s) 417
GluI GCNGC 1 cut(s) 417
GsaI CCCAGC 1 cut(s) 165
Hin1II CATG 4 cut(s) 29, 61, 287, 299
HinfI GANTC 1 cut(s) 5
HphI GGTGA 1 cut(s) 287
Hpy166II GTNNAC 1 cut(s) 370
Hpy188I TCNGA 2 cut(s) 79, 114
Hpy8I GTNNAC 1 cut(s) 370
HpyAV CCTTC 2 cut(s) 24, 163
HpyCH4III ACNGT 2 cut(s) 374, 397
HpyCH4V TGCA 2 cut(s) 61, 66
HpyF3I CTNAG 1 cut(s) 51
Hsp92II CATG 4 cut(s) 29, 61, 287, 299
Kzo9I GATC 1 cut(s) 298
LmnI GCTCC 1 cut(s) 307
LpnPI CCDG 8 cut(s) 20, 47, 147, 257, 261, 273, 287, 348
Lsp1109I GCAGC 1 cut(s) 403
MaeIII GTNAC 2 cut(s) 262, 397
MalI GATC 1 cut(s) 300
MboI GATC 1 cut(s) 298
MboII GAAGA 3 cut(s) 220, 242, 415
MhlI GDGCHC 2 cut(s) 201, 335
MluCI AATT 1 cut(s) 290
MnlI CCTC 2 cut(s) 79, 317
MseI TTAA 2 cut(s) 191, 224
MspR9I CCNGG 1 cut(s) 35
MvaI CCWGG 1 cut(s) 35
NdeII GATC 1 cut(s) 298
NlaIII CATG 4 cut(s) 29, 61, 287, 299
NlaIV GGNNCC 2 cut(s) 12, 147
NmuCI GTSAC 1 cut(s) 397
NspI RCATGY 1 cut(s) 29
NspV TTCGAA 1 cut(s) 17
PcsI WCGNNNNNNNCGW 1 cut(s) 14
PfeI GAWTC 1 cut(s) 5
PflMI CCANNNNNTGG 2 cut(s) 142, 301
PkrI GCNGC 1 cut(s) 418
PshBI ATTAAT 1 cut(s) 224
Psp6I CCWGG 1 cut(s) 33
PspFI CCCAGC 1 cut(s) 161
PspGI CCWGG 1 cut(s) 33
PspN4I GGNNCC 2 cut(s) 12, 147
PspPI GGNCC 2 cut(s) 10, 370
SaqAI TTAA 2 cut(s) 191, 224
SatI GCNGC 1 cut(s) 417
Sau3AI GATC 1 cut(s) 298
Sau96I GGNCC 2 cut(s) 10, 370
ScrFI CCNGG 1 cut(s) 35
SduI GDGCHC 2 cut(s) 201, 335
SetI ASST 6 cut(s) 36, 57, 90, 103, 155, 312
SfuI TTCGAA 1 cut(s) 17
SinI GGWCC 2 cut(s) 10, 370
Sse9I AATT 1 cut(s) 290
SsiI CCGC 2 cut(s) 305, 316
SspI AATATT 1 cut(s) 222
StyD4I CCNGG 1 cut(s) 33
StyI CCWWGG 1 cut(s) 213
TaaI ACNGT 2 cut(s) 374, 397
TaqI TCGA 1 cut(s) 17
TasI AATT 1 cut(s) 290
TfiI GAWTC 1 cut(s) 5
Tru1I TTAA 2 cut(s) 191, 224
Tru9I TTAA 2 cut(s) 191, 224
TscAI CASTG 2 cut(s) 68, 402
TseFI GTSAC 1 cut(s) 397
TseI GCWGC 1 cut(s) 416
Tsp45I GTSAC 1 cut(s) 397
TspDTI ATGAA 2 cut(s) 54, 272
TspRI CASTG 2 cut(s) 68, 402
Van91I CCANNNNNTGG 2 cut(s) 142, 301
VpaK11BI GGWCC 2 cut(s) 10, 370
VspI ATTAAT 1 cut(s) 224
XceI RCATGY 1 cut(s) 29
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.