Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0012868

Physical Location & Seq

Genomic Coordinates Forward (+)

37010706 .. 37012167

Gene Structure 1462 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1239200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 987 bp

ATGGGCCACCGCTGCTGCGGCAAACAGAAGGTGAAGAGAGGTCTATGGTCACCTGAAGAAGATGAGAAGCTCAGGAAACACATTACCACCCATGGTCATGGCAGCTGGAGTTCTGTCCCAAAACATGCAGGCTTGCAGAGGTGTGGCAAGAGTTGCAGGTTGAGATGGATAAACTATTTGAGACCTGAACTCAGGAGGGGATCCTTTACTCCTGAAGAAGAACAGACTATCATTGATGTTCACAGAATTTTGGGTAACAAATGGGCTCAGATTGCCAAGCATCTCCCTGGAAGAACAGACAATGAGGTGAAGAATTTCTGGAATTCATGCATTAAGAAAAAGCTCATGTCACAAGGTTTAGACCCAAAAACCCACAACTTGCTTCCTAAATCTCATCATCAGAGGTCAGCTTCTAACAACGCTGCAAGCAGCAGCCAATCTCCACTGCTACAGTCACATAATCAACAGCAACCCATTTCAGTACATTTAACTGAAATAAATGCATCACCTATTAATGTGACATTATCTACTGATCAATATCATGATCATGGTCAAAACCCTAGTAATGCTTCTTGGACTAGGGTTGACGATCCTACCTTCAATGACATCACCAGGTTGCCTCATCAACAATCCACTGATCACAGCACACTGATCAATAATATTTCCTCTTCATCGCCGTCTTCCTCGGTGAACCTATCAGTGTTTGGACTCTTGGAAAATAGTAACATCTGGGCTTCTGGTACTGAACCATTTGAAGCACCAAGAATATTAGTGGGAGAGCAGTCAAAGGGAGAAGAAGTAGTTCTACAGCAAGAAAAGGATACGCTCTTGGATCAGATGGAAATTAAGGGTATTGAGGATATGGATCATGCTTCAATATTTGAAAGTTCTAGTGTTGATTTTAGCTTTGTCGAGTCTACACTTATGTCGTGTGGAGAAATATCTCAGGATCTGAACTCTATGTGTAATTTTGCATGGAGATATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000981 GO:0000988 GO:0000989 GO:0001067 GO:0001076 GO:0001134 GO:0001135 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0006357 GO:0008150 GO:0009889 GO:0009987 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0030154 GO:0031323 GO:0031326 GO:0032502 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044212 GO:0044424 GO:0044464 GO:0048869 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0097159 GO:0140110 GO:1901363 GO:1903506 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Myb_DNA-binding

Protein Analysis

329

Amino Acids

36.97

Weight (kDa)

6.62

Isoelectric Point (pI)

67.56

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Myb_DNA-binding	PF00249	13 - 60	2.7e-16	Myb-like DNA-binding domain
Myb_DNA-bind_6	PF13921	16 - 76	2.9e-13	Myb-like DNA-binding domain
Myb_DNA-binding	PF00249	66 - 109	5.8e-18	Myb-like DNA-binding domain
Myb_DNA-bind_6	PF13921	69 - 114	1.1e-08	Myb-like DNA-binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0012868)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G12720
fragaria_vesca	FvH4_3g13990
malus_domestica	MD05G1239200.v1.1 MD10G1217300.v1.1
prunus_persica	Prupe.4G126900_v2.0.a1
pyrus_communis	pycom05g21550 pycom10g18330
rosa_chinensis	RchiOBHm_Chr5g0023221
rosa_laevigata	RLG00000032710
rosa_multiflora	Rmu_sc0001735.1_g000008 Rmu_sc0007300.1_g000006
rosa_roxburghii	Rroxscaffold_1G00056110
rosa_rugosa	Rorug05G0078100
rosa_samantha	Rh5AG164200 Rh5BG163700 Rh5CG178900 Rh5DG165500
rosa_wichuraiana	Rw5G014830

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	147
AccI	GTMKAC	1 cut(s)	917
AciI	CCGC	2 cut(s)	10, 18
AclWI	GGATC	6 cut(s)	195, 208, 584, 840, 873, 957
AcsI	RAATTY	3 cut(s)	246, 313, 322
AcuI	CTGAAG	2 cut(s)	75, 234
AfaI	GTAC	2 cut(s)	483, 742
AgsI	TTSAA	4 cut(s)	601, 755, 876, 884
AjnI	CCWGG	2 cut(s)	286, 611
AluBI	AGCT	5 cut(s)	70, 105, 343, 410, 906
AluI	AGCT	5 cut(s)	70, 105, 343, 410, 906
Alw26I	GTCTC	1 cut(s)	175
AlwI	GGATC	6 cut(s)	195, 208, 584, 840, 873, 957
AlwNI	CAGNNNCTG	1 cut(s)	952
AoxI	GGCC	1 cut(s)	4
ApeKI	GCWGC	6 cut(s)	12, 15, 102, 422, 429, 432
ApoI	RAATTY	3 cut(s)	246, 313, 322
AseI	ATTAAT	1 cut(s)	513
Asp700I	GAANNNNTTC	2 cut(s)	314, 801
AspS9I	GGNCC	1 cut(s)	4
AsuHPI	GGTGA	6 cut(s)	42, 43, 319, 498, 601, 700
BamHI	GGATCC	1 cut(s)	200
BanII	GRGCYC	1 cut(s)	268
BarI	GAAGNNNNNNTAC	2 cut(s)	789, 821
BbsI	GAAGAC	1 cut(s)	672
BbvI	GCAGC	5 cut(s)	2, 114, 409, 441, 444
BccI	CCATC	2 cut(s)	159, 832
BceAI	ACGGC	1 cut(s)	661
BciT130I	CCWGG	2 cut(s)	288, 613
BciVI	GTATCC	1 cut(s)	814
BclI	TGATCA	4 cut(s)	532, 544, 637, 651
BcoDI	GTCTC	1 cut(s)	175
BfaI	CTAG	3 cut(s)	561, 579, 891
BfmI	CTRYAG	2 cut(s)	449, 806
BfuAI	ACCTGC	1 cut(s)	147
BfuI	GTATCC	1 cut(s)	814
BisI	GCNGC	7 cut(s)	13, 16, 19, 103, 423, 430, 433
BlsI	GCNGC	7 cut(s)	14, 17, 20, 104, 424, 431, 434
Bme1390I	CCNGG	2 cut(s)	288, 613
BmgT120I	GGNCC	1 cut(s)	4
BmiI	GGNNCC	1 cut(s)	202
BmrFI	CCNGG	2 cut(s)	288, 613
BmsI	GCATC	2 cut(s)	289, 512
BpiI	GAAGAC	1 cut(s)	672
BpmI	CTGGAG	1 cut(s)	127
Bpu10I	CCTNAGC	1 cut(s)	71
BsaBI	GATNNNNATC	2 cut(s)	537, 864
BsaI	GGTCTC	1 cut(s)	175
BsaJI	CCNNGG	3 cut(s)	91, 286, 684
BsaXI	ACNNNNNCTCC	2 cut(s)	783, 813
Bse8I	GATNNNNATC	2 cut(s)	537, 864
BseBI	CCWGG	2 cut(s)	288, 613
BseDI	CCNNGG	3 cut(s)	91, 286, 684
BseJI	GATNNNNATC	2 cut(s)	537, 864
BseMII	CTCAG	4 cut(s)	85, 205, 281, 959
BseXI	GCAGC	5 cut(s)	2, 114, 409, 441, 444
BshFI	GGCC	1 cut(s)	6
BslFI	GGGAC	1 cut(s)	101
BsmAI	GTCTC	1 cut(s)	175
BsmFI	GGGAC	1 cut(s)	101
BsnI	GGCC	1 cut(s)	6
Bso31I	GGTCTC	1 cut(s)	175
Bsp1286I	GDGCHC	1 cut(s)	268
Bsp143I	GATC	9 cut(s)	200, 532, 544, 589, 637, 651, 832, 865, 949
Bsp19I	CCATGG	1 cut(s)	91
BspACI	CCGC	2 cut(s)	10, 18
BspANI	GGCC	1 cut(s)	6
BspCNI	CTCAG	4 cut(s)	84, 204, 280, 958
BspHI	TCATGA	1 cut(s)	541
BspLI	GGNNCC	1 cut(s)	202
BspMI	ACCTGC	1 cut(s)	147
BspPI	GGATC	6 cut(s)	195, 208, 584, 840, 873, 957
BspTNI	GGTCTC	1 cut(s)	175
BssECI	CCNNGG	3 cut(s)	91, 286, 684
BssMI	GATC	9 cut(s)	200, 532, 544, 589, 637, 651, 832, 865, 949
BssT1I	CCWWGG	1 cut(s)	91
Bst2UI	CCWGG	2 cut(s)	288, 613
Bst4CI	ACNGT	1 cut(s)	453
Bst6I	CTCTTC	2 cut(s)	29, 673
BstAPI	GCANNNNNTGC	1 cut(s)	153
BstC8I	GCNNGC	3 cut(s)	130, 134, 427
BstDEI	CTNAG	4 cut(s)	71, 191, 267, 945
BstDSI	CCRYGG	1 cut(s)	91
BstEII	GGTNACC	1 cut(s)	48
BstKTI	GATC	9 cut(s)	203, 535, 547, 592, 640, 654, 835, 868, 952
BstMAI	GTCTC	1 cut(s)	175
BstMBI	GATC	9 cut(s)	200, 532, 544, 589, 637, 651, 832, 865, 949
BstMWI	GCNNNNNNNGC	4 cut(s)	12, 18, 153, 272
BstNI	CCWGG	2 cut(s)	288, 613
BstNSI	RCATGY	1 cut(s)	128
BstPI	GGTNACC	1 cut(s)	48
BstSCI	CCNGG	2 cut(s)	286, 611
BstSFI	CTRYAG	2 cut(s)	449, 806
BstV1I	GCAGC	5 cut(s)	2, 114, 409, 441, 444
BstV2I	GAAGAC	1 cut(s)	672
BstX2I	RGATCY	2 cut(s)	200, 949
BstXI	CCANNNNNNTGG	1 cut(s)	98
BstYI	RGATCY	2 cut(s)	200, 949
BsuI	GTATCC	1 cut(s)	814
BsuRI	GGCC	1 cut(s)	6
BtgI	CCRYGG	1 cut(s)	91
BtgZI	GCGATG	1 cut(s)	657
BtsI	GCAGTG	1 cut(s)	443
BtsIMutI	CAGTG	4 cut(s)	443, 633, 647, 705
BveI	ACCTGC	1 cut(s)	147
Cac8I	GCNNGC	3 cut(s)	130, 134, 427
CaiI	CAGNNNCTG	1 cut(s)	952
CciI	TCATGA	1 cut(s)	541
Cfr13I	GGNCC	1 cut(s)	4
CsiI	ACCWGGT	1 cut(s)	611
Csp6I	GTAC	2 cut(s)	482, 741
CviAII	CATG	9 cut(s)	92, 98, 125, 327, 346, 542, 548, 869, 975
CviQI	GTAC	2 cut(s)	482, 741
DdeI	CTNAG	4 cut(s)	71, 191, 267, 945
DpnI	GATC	9 cut(s)	202, 534, 546, 591, 639, 653, 834, 867, 951
DpnII	GATC	9 cut(s)	200, 532, 544, 589, 637, 651, 832, 865, 949
Eam1104I	CTCTTC	2 cut(s)	29, 673
EarI	CTCTTC	2 cut(s)	29, 673
Eco130I	CCWWGG	1 cut(s)	91
Eco24I	GRGCYC	1 cut(s)	268
Eco31I	GGTCTC	1 cut(s)	175
Eco57I	CTGAAG	2 cut(s)	75, 234
Eco91I	GGTNACC	1 cut(s)	48
EcoO65I	GGTNACC	1 cut(s)	48
EcoRI	GAATTC	1 cut(s)	322
EcoRII	CCWGG	2 cut(s)	286, 611
EcoT14I	CCWWGG	1 cut(s)	91
EcoT22I	ATGCAT	2 cut(s)	332, 505
EcoT38I	GRGCYC	1 cut(s)	268
ErhI	CCWWGG	1 cut(s)	91
FaeI	CATG	9 cut(s)	95, 101, 128, 330, 349, 545, 551, 872, 978
FaqI	GGGAC	1 cut(s)	101
FatI	CATG	9 cut(s)	91, 97, 124, 326, 345, 541, 547, 868, 974
FbaI	TGATCA	4 cut(s)	532, 544, 637, 651
FblI	GTMKAC	1 cut(s)	917
Fnu4HI	GCNGC	7 cut(s)	13, 16, 19, 103, 423, 430, 433
FriOI	GRGCYC	1 cut(s)	268
Fsp4HI	GCNGC	7 cut(s)	13, 16, 19, 103, 423, 430, 433
FspBI	CTAG	3 cut(s)	561, 579, 891
GluI	GCNGC	7 cut(s)	13, 16, 19, 103, 423, 430, 433
GsuI	CTGGAG	1 cut(s)	127
HaeIII	GGCC	1 cut(s)	6
Hin1II	CATG	9 cut(s)	95, 101, 128, 330, 349, 545, 551, 872, 978
HincII	GTYRAC	1 cut(s)	586
HindII	GTYRAC	1 cut(s)	586
HinfI	GANTC	2 cut(s)	708, 914
HphI	GGTGA	6 cut(s)	42, 43, 319, 498, 601, 700
Hpy166II	GTNNAC	4 cut(s)	241, 586, 691, 918
Hpy188I	TCNGA	4 cut(s)	270, 402, 837, 954
Hpy188III	TCNNGA	6 cut(s)	73, 193, 212, 319, 542, 947
Hpy8I	GTNNAC	4 cut(s)	241, 586, 691, 918
HpyAV	CCTTC	2 cut(s)	22, 607
HpyCH4III	ACNGT	1 cut(s)	453
HpyCH4V	TGCA	7 cut(s)	128, 136, 156, 330, 425, 503, 974
HpyF10VI	GCNNNNNNNGC	4 cut(s)	12, 18, 153, 272
HpyF3I	CTNAG	4 cut(s)	71, 191, 267, 945
Hsp92II	CATG	9 cut(s)	95, 101, 128, 330, 349, 545, 551, 872, 978
Ksp22I	TGATCA	4 cut(s)	532, 544, 637, 651
Kzo9I	GATC	9 cut(s)	200, 532, 544, 589, 637, 651, 832, 865, 949
Lsp1109I	GCAGC	5 cut(s)	2, 114, 409, 441, 444
LweI	GCATC	2 cut(s)	289, 512
MabI	ACCWGGT	1 cut(s)	611
MaeI	CTAG	3 cut(s)	561, 579, 891
MaeIII	GTNAC	6 cut(s)	48, 254, 348, 453, 517, 722
MalI	GATC	9 cut(s)	202, 534, 546, 591, 639, 653, 834, 867, 951
MboI	GATC	9 cut(s)	200, 532, 544, 589, 637, 651, 832, 865, 949
MflI	RGATCY	2 cut(s)	200, 949
MhlI	GDGCHC	1 cut(s)	268
MluCI	AATT	5 cut(s)	246, 313, 322, 843, 967
MlyI	GAGTC	2 cut(s)	702, 923
MnlI	CCTC	9 cut(s)	32, 132, 189, 298, 396, 630, 676, 694, 850
Mph1103I	ATGCAT	2 cut(s)	332, 505
MroXI	GAANNNNTTC	2 cut(s)	314, 801
MseI	TTAA	4 cut(s)	333, 488, 513, 846
MslI	CAYNNNNRTG	2 cut(s)	96, 546
MspA1I	CMGCKG	2 cut(s)	12, 105
MspR9I	CCNGG	2 cut(s)	288, 613
MvaI	CCWGG	2 cut(s)	288, 613
MwoI	GCNNNNNNNGC	4 cut(s)	12, 18, 153, 272
NcoI	CCATGG	1 cut(s)	91
NdeII	GATC	9 cut(s)	200, 532, 544, 589, 637, 651, 832, 865, 949
NlaIII	CATG	9 cut(s)	95, 101, 128, 330, 349, 545, 551, 872, 978
NlaIV	GGNNCC	1 cut(s)	202
NmuCI	GTSAC	4 cut(s)	48, 348, 453, 517
NsiI	ATGCAT	2 cut(s)	332, 505
NspI	RCATGY	1 cut(s)	128
PagI	TCATGA	1 cut(s)	541
PdmI	GAANNNNTTC	2 cut(s)	314, 801
PkrI	GCNGC	7 cut(s)	14, 17, 20, 104, 424, 431, 434
PleI	GAGTC	2 cut(s)	702, 922
PpsI	GAGTC	2 cut(s)	702, 922
PshBI	ATTAAT	1 cut(s)	513
Psp6I	CCWGG	2 cut(s)	286, 611
PspEI	GGTNACC	1 cut(s)	48
PspGI	CCWGG	2 cut(s)	286, 611
PspN4I	GGNNCC	1 cut(s)	202
PspPI	GGNCC	1 cut(s)	4
PstNI	CAGNNNCTG	1 cut(s)	952
PsuI	RGATCY	2 cut(s)	200, 949
PvuII	CAGCTG	1 cut(s)	105
RsaI	GTAC	2 cut(s)	483, 742
RsaNI	GTAC	2 cut(s)	482, 741
RseI	CAYNNNNRTG	2 cut(s)	96, 546
SaqAI	TTAA	4 cut(s)	333, 488, 513, 846
SatI	GCNGC	7 cut(s)	13, 16, 19, 103, 423, 430, 433
Sau3AI	GATC	9 cut(s)	200, 532, 544, 589, 637, 651, 832, 865, 949
Sau96I	GGNCC	1 cut(s)	4
SchI	GAGTC	2 cut(s)	702, 923
ScrFI	CCNGG	2 cut(s)	288, 613
SduI	GDGCHC	1 cut(s)	268
SexAI	ACCWGGT	1 cut(s)	611
SfaNI	GCATC	2 cut(s)	289, 512
SfcI	CTRYAG	2 cut(s)	449, 806
SmiMI	CAYNNNNRTG	2 cut(s)	96, 546
Sse9I	AATT	5 cut(s)	246, 313, 322, 843, 967
SsiI	CCGC	2 cut(s)	10, 18
SspI	AATATT	3 cut(s)	661, 768, 879
SspMI	CTAG	3 cut(s)	561, 579, 891
StyD4I	CCNGG	2 cut(s)	286, 611
StyI	CCWWGG	1 cut(s)	91
TaaI	ACNGT	1 cut(s)	453
TaqI	TCGA	1 cut(s)	912
TasI	AATT	5 cut(s)	246, 313, 322, 843, 967
TatI	WGTACW	1 cut(s)	481
TauI	GCSGC	1 cut(s)	21
Tru1I	TTAA	4 cut(s)	333, 488, 513, 846
Tru9I	TTAA	4 cut(s)	333, 488, 513, 846
TscAI	CASTG	4 cut(s)	450, 640, 654, 705
TseFI	GTSAC	4 cut(s)	48, 348, 453, 517
TseI	GCWGC	6 cut(s)	12, 15, 102, 422, 429, 432
Tsp45I	GTSAC	4 cut(s)	48, 348, 453, 517
TspDTI	ATGAA	2 cut(s)	315, 660
TspRI	CASTG	4 cut(s)	450, 640, 654, 705
VspI	ATTAAT	1 cut(s)	513
XapI	RAATTY	3 cut(s)	246, 313, 322
XceI	RCATGY	1 cut(s)	128
XmiI	GTMKAC	1 cut(s)	917
XmnI	GAANNNNTTC	2 cut(s)	314, 801
XspI	CTAG	3 cut(s)	561, 579, 891
Zsp2I	ATGCAT	2 cut(s)	332, 505

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.