Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0006854

Physical Location & Seq

Genomic Coordinates Forward (+)

40907989 .. 40924095

Gene Structure 16107 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1274500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 5142 bp

ATGGAGGACAATTTAGTTGTGGGGGGCGAAGAGACGGTTGAAAAGGCCCCAATTTCGGAGAAACAGGCCTCTGTTCGTTCATCAGAAACTGCAAAAGGAGTTACCAAAACTGTGAAAACCGGCAGTGCAGTGACATCAAAGGTCTCGGTTCCAACTGTTAGAAAGAAAGTAGACCCCAAAAGTGGTGTGGATCCTAGTTCAAGGGCTACCAAGTCTAGTGCAAATGGGTCTACCCGAAGTTTGAATTCAGTTCCGATAAGGCGAAACAGCACTGGAGGTTTGCCGCAGAAGCCATCAGTCTCCACCACAAGACAGCAAAACAATGCCACTACGACTGCAGTAAAAAAGACTGTGGACCCTGTCAGGCGGTCTCTCCCAGAGCTTAGAAGGAGTTCATTGCCTTCAGCTGCTGCTACTAAATCTTCGACTAGAATCAGTGTTTCAGAAGTTAGAAAGTCGGTTTCAGGATCGCCATTGGAGAGGAGTCTGAATAAATCATCCGGGTCAAATGTGAGTGTAACTAAACAAGAGACTGTCAGGAAGCCTGCTGTCAAACCTGCATTGTCCGCTTCATCTTCCTCTTCAAGAAGGGCGACTTCCTCATTGGATAGTAGTGCTAGCAGTGGAGTCAGGAAGTCGGTCTCAAAGCTTTCTTCCCCATTAGCTCGAACACCTACAGTTACTAGTAGGCTGAAAACCGGTTCTTTGTCCTCTTCTCTTGATAGAAGTTCTAGTTTATCTGGGCGTAGGAAAACTGCAACTCATGAAAGCCGGGATTCTCGGTTCATTGTCCTTCCACAAGTGGAAATCAAAGCCAGTGATGATCTGAGGTTAGATCTTAGGGGTCACAGAGTTCGCAGCCTCAAGGCTAATGGGTTGAACTTGTCACCAAATTTAGAGTTCGTTTATCTCAGAGACAATCTCTTGTCTACATTGGAGGGAGTGGAAATACTGGCACGAGTAAAGGTTCTTGATTTGAGTTTCAATGATTTCAAAGGGCCTGGATTTGAACCTCTTGAAACTTGCAAAGTCTTACAGCAACTTTATCTTGCTGGAAATCAACTCACGTCACTTGCAAGCCTTCCTCAGCTCCCTAATTTGGAGTTTCTCTCTGTCGCTCAAAATAAATTGAAGTCTCTTACAATGGCAAGCCAACCTAGACTACAGGTATTAGCTGCCAGCAAGAACAAAATTTCAACTCTAAAAGGTTTCCCCTATTTACCGGTCCTTGAGCATTTACGAGTGGAGGAAAATCCCATACTTAAAATGCCTCACCTAGAAGCAGCTTCCATTTTGCTTGTTGGTCCTACATTAAAAAAGTTCAATGACAGAGATCTTTCCCGAGAAGAGCTTACAATTGCAAAGCGCTATCCGGCACACACCTCTTTGTGCATTAGAGATGGTTGGGAGTTTTGCCGTCCAGATCATGCAGCAGACTCTACTTTTCGTTTTCTTGTTGAGCAATGGAAGGATCATTTGCCTCCCGGCTTCCTGGTCAAGAAGGTCTCTGTTGAGAAACCATTTGAAGAAGATACTTGTCGCTGCCAGTTCACTTTTGTACAAGAGAACACTTTGGCAACTGATCCACAATTAATCTTGAAATACCAATGGTTTGTAGGAGAAAGAACTCCTTCGAATTTCACCATTATTCCTGATGCCACTGGAGAGGTCTACTGGCCTAAGCATGAAGATATCGGTAGAATTTTAAAAGTGGAGTGTACTCCTGTATTAGGAGAAACGGAGTACCCTTCTATTTTTGCTATATCTTCTCCTGTTAAACGAGGAAGCAGAATCCCAAAGGTTGTGAATCTTGATGTTCATGGAAATCTAGTGGAGGGAAATATTGTTAAAGGTCATGCAGAAGTTGCGTGGTGTGGTGGCACTCCAGGGAAAGGTGTTTCTAGTTGGTTGAGGAGAAAATGGAATAGCAGTCCTGTGGTTATTGCGGGAGCTGAAGATGAGGAGTACAAATTGACCATAGATGATATTGACTCAAGTTTGGTTTTCATGTATACACCAGTGACAGAAGAAGGTGCCAAAGGAGAACCTCATTATGAATACACTGATTTTGTAAAATCAGCCCCTCCATCAGTCAATAATGTACACATTGTTGGAGATGTTGTTGAAGGCAGTACTATCAGAGGAGTTGGTGATTACTTTGGAGGAAGAGAGGGTCCCAGCAAGTTTGAATGGTTATGTGAGGATAAAGACACTGGAGACCTTGTTTTGTTATCAACGGGTACACCTGAGTATACTTTGACCAAGGAAGATGTTGGCCATCGCTTGGTTTTTGTGTATATACCCATCAATTTTGAAGGTCAAGAAGGGGAATCTGTTTCGATAAGGTCCCAAGTTGTGAAGCCAGCGCCTCCAAAGGTAATAGATTTGAAGATAGTTGGTGATCTAAGGGAGAACAGTAAGGTTACTGCAACCGGTACTGTCACTGGAGGAACTGAAGGGTCTAGCAGAGTACAGTGGTTTAAAACAAGTTCGTCTACTTTGGATGGTGAGAAAGGCCTTGAATCTTTAAGCACATCTAAGATTGCAAAGGCTTTTCGCGTACCCTTAGGAGCTGTTGGCTATTACATTGTTGTAAAATTTACTCCTATGACTCCAGATGGTGAATCTGGTGAACCAGCATATGCCATATCAGATAGAGCAGTTGAGACTCTGCCTCCAAGCCTAAATTTCTTATCAATTACTGGTGATTACACCGAAGGTGGAATGTTGACGGCATCATATGGTTACATTGGTGGTCATGAAGGAAAAAGTACCTGTAGTTGGTATCTTCACGAGGTTGAAACTGACTCCGGATCTCTAATACCTGAGGTCACGGGGCTTCTTCAGTATCGTATTGCAAAAGATGCAATTGGTAAATTCATTTCATTCAAATGCACCCCAGTGCGTGATGATGGGATTGTGGGTGAGCCAAGAACATGCATGGGCCAGGAACGTGTTCGCCCTGGGAACCCTAGATTGCTTTCTCTACAAATCGTTGGGAATGCCACTGAGGGAACTGCACTTAGTGTAGAAAAGAAATATTGGGGTGGTGAAGAGGGAAATTCAGTTTTCTACTGGTTCCGGACTAATTCAGATGGAACACAAACCGAAATCAGGGGTGCAACAACTGCTTCCTACACACTCTCAATTGATGATATTGGCTTCTTTGTCTCAGTATCTTGTGAGCCTGTCAGAAGTGACTGGGCACGTGGTCCTACTGTTCTATCCGAACACATTGGACCTGTAATACCTGGGCCCCCAACTTGTCGGTCTTTGGAGTTTCTTGGTTCAATGATAGAAGGGCAACGTCTGAGCTTTACTGCTTCTTATAGTGGAGGGGAGCAAGGCGATTGCTCGCATGAATGGTTTAGAGTTAAACGTAATGGTGTCAAGGAGAAGTTGAGTGCTCAGGATTTTGTGGATTTGACTCTTGATGATGTGGGTACATGTGTTGAACTTGTTTACACTCCCATGCGCAAAGATGGGATGAGAGGAAATCCTAAGAGCATACAATCTGATGTGATTGCTCCTGCGGATCCAGTAGGACTGGAGCTTGTTATTCCCGATTGCTGTGAGAATGAGAATTTATTCCCCCAAAAAACATATTTTGGTGGAGAAGAAGGTGTTGGGGAATATATTTGGTACAGAACAAAGAATAAGCTGCATGGATCTGCATTGCAGGACATATATAATGCATGTGAAGATTTGGTTATTTGTGGTAAAACATTAACATACACACCAGTACTTGAAGATGTGGGTGCATATTTGGCCTTACATTGGGTCCCCACTCGTTCTGATGGCAAATGTGGACAGGCACTAATTGCCATTTGCAACTTTCCAGTTGCACCAGCTCTTCCTGTGGTTTCTAATGTTCGTGCGAAGGAGTTATCACAAAGCATTTATTCTGGAGAAGGAGAGTATTTTGGTGGATATGAAGGATCGAGCCTGTTTAGCTGGTATAGAGAAACCAATGAAGGAACTATTGTTCTTATTAATGGAGCCAACACTAACACTTATGAAGTCACTGATGCAGACTACAACTGCCGTCTATTGTTTGGATACACACCAGTTCGATCAGATTCGGTGGTTGGAGAACTGAAGTTGTCTGAGCCAACTGATATTATTCTTCCAGAGCTTCCAAGACTAGAGATGCTTGCTCTCACGGGAAAGGCAATCGAAGGTGATGTTCTTACAGTTGTTCAAGTGATCCCAGAGAGTGAGACTCAACAGATTGTTTGGAGCAAGTACAAGAAAGATGTCAGATATCAATGGTATTTTTCTTCAAAAGAGGGGGATGAAAAAACCTTCGAAATATTACCTCCACAGCAATCTTGCTCCTACAAGATGCGTCTAGAAGATGTTGGGCGCTGTCTAAAATGCGAATGCGTTGTGACTGATGTTTTTGGAAGATCAACTGAGCCTGTCTATGCTGAAACCGGACCCATATTACCAGGAATCCCTAGAATAGATAAGCTGGAGATTGAAGGGAGGGGTTTCCACACCAACTTATACGCCGTACGTGGAATTTATAGTGGAGGGAAGGAAGGAAAAAGTAGAATCCAGTGGCTCAGATCAATGGTTGGCAGTCCTGATCTTATCTCTATAGCAGGGGAGGTAGGGCGAATGTACGAGTCTAATGTTGACGACGTAGGGTACAGGCTTGTTGCCATTTACACTCCTGTAAGGGAGGATGGAGTGGAGGGGCATCCTGTTTCTGCATCAACGGAGCCGATTGCAGTTGAACCTGATGTTCTTAAAGAAGTGAAGCAGAAGCTTGATCTTGGAACTGTGAAATTTGAGACATTGTGTGACAAGGACCAATCTACGAAAAAGGCTCCTGCAGTGGGAAGTCTTGAGAGAAGAATCCTCGAAGTCAACAGAAAAAGAGTCAAGGTGGTAAAGCCCGGTTCTAAGACTTCCTTCCCAACCACTGAAATTCGTGGAAGTTATGCGCCTCCCTTTCATGTGGAGCTGTTCCGAAACGACCAACACCGTCTGAAAATCGTCGTGGACGGCGAGAATAATGTCGACCTGATGGTGCAGTCGAGGCACCTTCGGGATGTAACCGTGCTGGTGATCAGAGGGCTTGCTCAGAAATTCAACAGTACATCACTCAATACCCTCCTCAAGATAGAGGCTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000226 GO:0001578 GO:0001669 GO:0002177 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005856 GO:0006996 GO:0007010 GO:0007017 GO:0007276 GO:0007283 GO:0008150 GO:0009987 GO:0012505 GO:0015630 GO:0016043 GO:0019953 GO:0022414 GO:0022607 GO:0030030 GO:0030031 GO:0030141 GO:0031410 GO:0031982 GO:0032501 GO:0032504 GO:0035082 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043232 GO:0044085 GO:0044422 GO:0044424 GO:0044430 GO:0044444 GO:0044446 GO:0044464 GO:0044703 GO:0044782 GO:0048232 GO:0048609 GO:0051704 GO:0060271 GO:0070925 GO:0071840 GO:0097223 GO:0097708 GO:0099503 GO:0120031 GO:0120036

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

LRR_4

Protein Analysis

1714

Amino Acids

187.88

Weight (kDa)

6.63

Isoelectric Point (pI)

38.76

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LRR_4	PF12799	345 - 381	2.7e-06	Leucine Rich repeats (2 copies)
IG_AIR9	PF23197	504 - 589	2.5e-10	AIR9 A9 domain
IG_AIR9	PF23197	601 - 685	2.7e-21	AIR9 A9 domain
IG_AIR9	PF23197	697 - 782	2.7e-33	AIR9 A9 domain
IG_AIR9	PF23197	793 - 884	1.4e-16	AIR9 A9 domain
IG_AIR9	PF23197	895 - 981	9.5e-22	AIR9 A9 domain
IG_AIR9	PF23197	995 - 1074	5.2e-22	AIR9 A9 domain
IG_AIR9	PF23197	1089 - 1171	4.6e-23	AIR9 A9 domain
IG_AIR9	PF23197	1186 - 1273	3.5e-15	AIR9 A9 domain
IG_AIR9	PF23197	1297 - 1367	1.5e-18	AIR9 A9 domain
IG_AIR9	PF23197	1489 - 1574	2.8e-20	AIR9 A9 domain
PH_AIR9	PF23218	1604 - 1700	5.9e-52	AIR9 PH-like domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0006854)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G34680 AT2G34680
fragaria_vesca	FvH4_3g09950 FvH4_3g09950 FvH4_3g09950 FvH4_3g09950 FvH4_3g09950
malus_domestica	MD05G1274500.v1.1 MD10G1254200.v1.1
prunus_persica	Prupe.4G089200_v2.0.a1 Prupe.4G089200_v2.0.a1 Prupe.4G089200_v2.0.a1
pyrus_communis	pycom05g25590 pycom10g20990
rosa_chinensis	RchiOBHm_Chr5g0015701
rosa_laevigata	RLG00000032220
rosa_multiflora	Rmu_sc0000843.1_g000009
rosa_roxburghii	Rroxscaffold_1G00060820
rosa_rugosa	Rorug05G0029200 Rorug05G0029200 Rorug05G0029300
rosa_samantha	Rh5AG122500 Rh5BG121800 Rh5CG132000 Rh5DG120500
rosa_wichuraiana	Rw5G010580

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	3476
Acc36I	ACCTGC	1 cut(s)	565
AccB1I	GGYRCC	2 cut(s)	2033, 5049
AccB7I	CCANNNNNTGG	1 cut(s)	2284
AccI	GTMKAC	8 cut(s)	171, 230, 929, 1671, 2012, 2252, 2495, 5028
AccII	CGCG	1 cut(s)	2559
AccIII	TCCGGA	2 cut(s)	2810, 3081
AciI	CCGC	5 cut(s)	284, 367, 567, 1946, 3533
AcoI	YGGCCR	1 cut(s)	2275
AcuI	CTGAAG	5 cut(s)	387, 1974, 2475, 2828, 4118
AcvI	CACGTG	1 cut(s)	3209
AdeI	CACNNNGTG	1 cut(s)	3026
AfeI	AGCGCT	1 cut(s)	1367
AflIII	ACRYGT	2 cut(s)	2953, 3446
AgeI	ACCGGT	3 cut(s)	698, 1222, 2432
AhlI	ACTAGT	1 cut(s)	683
AjiI	CACGTC	1 cut(s)	1068
AjnI	CCWGG	7 cut(s)	1000, 1491, 1885, 2946, 2962, 3250, 4450
AjuI	GAANNNNNNNTTGG	2 cut(s)	3609, 3641
AleI	CACNNNNGTG	1 cut(s)	2900
AloI	GAACNNNNNNTCC	4 cut(s)	767, 799, 3969, 4001
Alw21I	GWGCWC	1 cut(s)	3409
AlwNI	CAGNNNCTG	2 cut(s)	89, 410
Ama87I	CYCGRG	1 cut(s)	1341
Aor13HI	TCCGGA	2 cut(s)	2810, 3081
Aor51HI	AGCGCT	1 cut(s)	1367
AoxI	GGCC	9 cut(s)	45, 66, 998, 1676, 2275, 2515, 2944, 3254, 3768
ApaI	GGGCCC	1 cut(s)	3258
ApeKI	GCWGC	8 cut(s)	407, 410, 858, 1175, 1283, 1430, 1542, 3661
AseI	ATTAAT	2 cut(s)	1592, 3993
AsiGI	ACCGGT	3 cut(s)	698, 1222, 2432
Asp700I	GAANNNNTTC	4 cut(s)	391, 1630, 2955, 3587
AspLEI	GCGC	5 cut(s)	1368, 2368, 3477, 4368, 4954
AsuC2I	CCSGG	4 cut(s)	502, 773, 1485, 4905
AsuII	TTCGAA	2 cut(s)	1634, 4308
AsuNHI	GCTAGC	1 cut(s)	617
AvaI	CYCGRG	1 cut(s)	1341
AxyI	CCTNAGG	2 cut(s)	2566, 2826
BaeGI	GKGCMC	2 cut(s)	3208, 3258
BaeI	ACNNNNGTAYC	2 cut(s)	3435, 3468
BalI	TGGCCA	1 cut(s)	2277
BamHI	GGATCC	2 cut(s)	190, 3535
BanI	GGYRCC	2 cut(s)	2033, 5049
BanII	GRGCYC	1 cut(s)	3258
BauI	CACGAG	2 cut(s)	957, 2792
BbrPI	CACGTG	1 cut(s)	3209
Bbv12I	GWGCWC	1 cut(s)	3409
BbvCI	CCTCAGC	1 cut(s)	1086
BbvI	GCAGC	8 cut(s)	394, 397, 870, 1162, 1295, 1442, 1529, 3648
BceAI	ACGGC	5 cut(s)	1401, 2748, 4029, 4499, 5029
BciT130I	CCWGG	7 cut(s)	1002, 1493, 1887, 2948, 2964, 3252, 4452
BciVI	GTATCC	1 cut(s)	4052
BclI	TGATCA	1 cut(s)	5076
BcnI	CCSGG	4 cut(s)	502, 773, 1485, 4905
BcuI	ACTAGT	1 cut(s)	683
BfmI	CTRYAG	6 cut(s)	336, 675, 1163, 2776, 4602, 4839
BfoI	RGCGCY	3 cut(s)	1369, 2369, 4369
BfuAI	ACCTGC	1 cut(s)	565
BfuI	GTATCC	1 cut(s)	4052
BglII	AGATCT	2 cut(s)	835, 1333
BisI	GCNGC	9 cut(s)	284, 408, 411, 859, 1176, 1284, 1431, 1543, 3662
BlsI	GCNGC	9 cut(s)	285, 409, 412, 860, 1177, 1285, 1432, 1544, 3663
BmcAI	AGTACT	2 cut(s)	2134, 3744
BmeT110I	CYCGRG	1 cut(s)	1341
BmgBI	CACGTC	1 cut(s)	1068
BmrI	ACTGGG	2 cut(s)	2894, 3211
BmsI	GCATC	8 cut(s)	1645, 2744, 2854, 4018, 4140, 4335, 4714, 4727
BmtI	GCTAGC	1 cut(s)	621
BmuI	ACTGGG	2 cut(s)	2894, 3211
BplI	GAGNNNNNCTC	6 cut(s)	906, 938, 1094, 1126, 4207, 4239
BpmI	CTGGAG	9 cut(s)	294, 1683, 1869, 2235, 2466, 2598, 3569, 3927, 4496
Bpu10I	CCTNAGC	3 cut(s)	1086, 1680, 3408
Bpu14I	TTCGAA	2 cut(s)	1634, 4308
BpuEI	CTTGAG	5 cut(s)	848, 1250, 1978, 4874, 5111
BpuMI	CCSGG	4 cut(s)	502, 773, 1485, 4905
BsaAI	YACGTR	2 cut(s)	3209, 4520
BsaI	GGTCTC	5 cut(s)	148, 375, 646, 1510, 2211
BsaJI	CCNNGG	5 cut(s)	1886, 2262, 2962, 2963, 3251
BsaWI	WCCGGW	6 cut(s)	698, 1222, 2432, 2810, 3081, 4436
BsaXI	ACNNNNNCTCC	6 cut(s)	2106, 2136, 2208, 2238, 2659, 2689
Bse118I	RCCGGY	4 cut(s)	119, 698, 1222, 2432
Bse21I	CCTNAGG	2 cut(s)	2566, 2826
Bse3DI	GCAATG	3 cut(s)	395, 1469, 3674
BseAI	TCCGGA	2 cut(s)	2810, 3081
BseBI	CCWGG	7 cut(s)	1002, 1493, 1887, 2948, 2964, 3252, 4452
BseDI	CCNNGG	5 cut(s)	1886, 2262, 2962, 2963, 3251
BseGI	GGATG	7 cut(s)	497, 2509, 3492, 4300, 4696, 4705, 5065
BseMI	GCAATG	3 cut(s)	395, 1469, 3674
BseRI	GAGGAG	5 cut(s)	496, 1927, 1976, 2157, 5114
BseSI	GKGCMC	2 cut(s)	3208, 3258
BseXI	GCAGC	8 cut(s)	394, 397, 870, 1162, 1295, 1442, 1529, 3648
BseYI	CCCAGC	1 cut(s)	2177
BsgI	GTGCAG	3 cut(s)	147, 3003, 5060
Bsh1236I	CGCG	1 cut(s)	2559
BshFI	GGCC	9 cut(s)	47, 68, 1000, 1678, 2277, 2517, 2946, 3256, 3770
BshNI	GGYRCC	2 cut(s)	2033, 5049
BshTI	ACCGGT	3 cut(s)	698, 1222, 2432
BsiHKAI	GWGCWC	1 cut(s)	3409
BsiHKCI	CYCGRG	1 cut(s)	1341
BsiWI	CGTACG	1 cut(s)	4516
BslFI	GGGAC	3 cut(s)	2160, 2332, 3767
BsmBI	CGTCTC	1 cut(s)	26
BsmFI	GGGAC	3 cut(s)	2160, 2332, 3767
BsmI	GAATGC	2 cut(s)	3007, 4388
BsnI	GGCC	9 cut(s)	47, 68, 1000, 1678, 2277, 2517, 2946, 3256, 3770
Bso31I	GGTCTC	5 cut(s)	148, 375, 646, 1510, 2211
BsoBI	CYCGRG	1 cut(s)	1341
Bsp119I	TTCGAA	2 cut(s)	1634, 4308
Bsp120I	GGGCCC	1 cut(s)	3254
Bsp1286I	GDGCHC	3 cut(s)	3208, 3258, 3409
Bsp13I	TCCGGA	2 cut(s)	2810, 3081
Bsp1407I	TGTACA	2 cut(s)	1558, 2101
BspACI	CCGC	5 cut(s)	284, 367, 567, 1946, 3533
BspANI	GGCC	9 cut(s)	47, 68, 1000, 1678, 2277, 2517, 2946, 3256, 3770
BspEI	TCCGGA	2 cut(s)	2810, 3081
BspFNI	CGCG	1 cut(s)	2559
BspHI	TCATGA	2 cut(s)	763, 2758
BspMAI	CTGCAG	2 cut(s)	340, 4843
BspMI	ACCTGC	1 cut(s)	565
BspOI	GCTAGC	1 cut(s)	621
BspQI	GCTCTTC	2 cut(s)	1341, 3858
BspT104I	TTCGAA	2 cut(s)	1634, 4308
BspT107I	GGYRCC	2 cut(s)	2033, 5049
BspTNI	GGTCTC	5 cut(s)	148, 375, 646, 1510, 2211
BsrDI	GCAATG	3 cut(s)	395, 1469, 3674
BsrFI	RCCGGY	4 cut(s)	119, 698, 1222, 2432
BsrGI	TGTACA	2 cut(s)	1558, 2101
BssAI	RCCGGY	4 cut(s)	119, 698, 1222, 2432
BssECI	CCNNGG	5 cut(s)	1886, 2262, 2962, 2963, 3251
BssNAI	GTATAC	2 cut(s)	2013, 2253
BssSI	CACGAG	2 cut(s)	957, 2792
BssT1I	CCWWGG	1 cut(s)	2262
Bst1107I	GTATAC	2 cut(s)	2013, 2253
Bst2BI	CACGAG	2 cut(s)	957, 2792
Bst2UI	CCWGG	7 cut(s)	1002, 1493, 1887, 2948, 2964, 3252, 4452
Bst6I	CTCTTC	7 cut(s)	24, 586, 718, 1341, 2161, 3048, 3858
BstAPI	GCANNNNNTGC	4 cut(s)	1865, 2864, 3128, 3821
BstAUI	TGTACA	2 cut(s)	1558, 2101
BstBAI	YACGTR	2 cut(s)	3209, 4520
BstBI	TTCGAA	2 cut(s)	1634, 4308
BstC8I	GCNNGC	9 cut(s)	546, 619, 1078, 1150, 1180, 2364, 3356, 4155, 5088
BstENI	CCTNNNNNAGG	1 cut(s)	1728
BstF5I	GGATG	7 cut(s)	497, 2509, 3492, 4300, 4696, 4705, 5065
BstFNI	CGCG	1 cut(s)	2559
BstH2I	RGCGCY	3 cut(s)	1369, 2369, 4369
BstHHI	GCGC	5 cut(s)	1368, 2368, 3477, 4368, 4954
BstMWI	GCNNNNNNNGC	9 cut(s)	289, 566, 1865, 2864, 3128, 3767, 3821, 3951, 5047
BstNI	CCWGG	7 cut(s)	1002, 1493, 1887, 2948, 2964, 3252, 4452
BstNSI	RCATGY	3 cut(s)	2940, 3450, 3699
BstSFI	CTRYAG	6 cut(s)	336, 675, 1163, 2776, 4602, 4839
BstSLI	GKGCMC	2 cut(s)	3208, 3258
BstUI	CGCG	1 cut(s)	2559
BstV1I	GCAGC	8 cut(s)	394, 397, 870, 1162, 1295, 1442, 1529, 3648
BstX2I	RGATCY	6 cut(s)	190, 835, 1333, 2813, 3535, 3668
BstYI	RGATCY	6 cut(s)	190, 835, 1333, 2813, 3535, 3668
BstZ17I	GTATAC	2 cut(s)	2013, 2253
Bsu36I	CCTNAGG	2 cut(s)	2566, 2826
BsuI	GTATCC	1 cut(s)	4052
BsuRI	GGCC	9 cut(s)	47, 68, 1000, 1678, 2277, 2517, 2946, 3256, 3770
BtgZI	GCGATG	1 cut(s)	2264
BtrI	CACGTC	1 cut(s)	1068
BtsCI	GGATG	7 cut(s)	497, 2509, 3492, 4300, 4696, 4705, 5065
BtsI	GCAGTG	4 cut(s)	130, 135, 628, 4848
BveI	ACCTGC	1 cut(s)	565
Cac8I	GCNNGC	9 cut(s)	546, 619, 1078, 1150, 1180, 2364, 3356, 4155, 5088
CaiI	CAGNNNCTG	2 cut(s)	89, 410
CciI	TCATGA	2 cut(s)	763, 2758
CfoI	GCGC	5 cut(s)	1368, 2368, 3477, 4368, 4954
Cfr10I	RCCGGY	4 cut(s)	119, 698, 1222, 2432
CseI	GACGC	1 cut(s)	4337
CspAI	ACCGGT	3 cut(s)	698, 1222, 2432
DraI	TTTAAA	2 cut(s)	1707, 2482
DraIII	CACNNNGTG	1 cut(s)	3026
EaeI	YGGCCR	1 cut(s)	2275
Eam1104I	CTCTTC	7 cut(s)	24, 586, 718, 1341, 2161, 3048, 3858
EarI	CTCTTC	7 cut(s)	24, 586, 718, 1341, 2161, 3048, 3858
Eco130I	CCWWGG	1 cut(s)	2262
Eco147I	AGGCCT	2 cut(s)	68, 2517
Eco24I	GRGCYC	1 cut(s)	3258
Eco31I	GGTCTC	5 cut(s)	148, 375, 646, 1510, 2211
Eco32I	GATATC	2 cut(s)	1693, 4265
Eco47III	AGCGCT	1 cut(s)	1367
Eco57I	CTGAAG	5 cut(s)	387, 1974, 2475, 2828, 4118
Eco72I	CACGTG	1 cut(s)	3209
Eco81I	CCTNAGG	2 cut(s)	2566, 2826
Eco88I	CYCGRG	1 cut(s)	1341
EcoNI	CCTNNNNNAGG	1 cut(s)	1728
EcoO109I	RGGNCCY	6 cut(s)	46, 998, 2174, 2346, 3255, 3781
EcoRI	GAATTC	1 cut(s)	244
EcoRII	CCWGG	7 cut(s)	1000, 1491, 1885, 2946, 2962, 3250, 4450
EcoRV	GATATC	2 cut(s)	1693, 4265
EcoT14I	CCWWGG	1 cut(s)	2262
EcoT22I	ATGCAT	2 cut(s)	2942, 3697
EcoT38I	GRGCYC	1 cut(s)	3258
ErhI	CCWWGG	1 cut(s)	2262
Esp3I	CGTCTC	1 cut(s)	26
FalI	AAGNNNNNCTT	6 cut(s)	580, 612, 1615, 1647, 4904, 4936
FaqI	GGGAC	3 cut(s)	2160, 2332, 3767
FauI	CCCGC	1 cut(s)	1939
FauNDI	CATATG	2 cut(s)	2641, 2740
FbaI	TGATCA	1 cut(s)	5076
FblI	GTMKAC	8 cut(s)	171, 230, 929, 1671, 2012, 2252, 2495, 5028
Fnu4HI	GCNGC	9 cut(s)	284, 408, 411, 859, 1176, 1284, 1431, 1543, 3662
FokI	GGATG	7 cut(s)	484, 2516, 3499, 4307, 4692, 4703, 5072
FriOI	GRGCYC	1 cut(s)	3258
Fsp4HI	GCNGC	9 cut(s)	284, 408, 411, 859, 1176, 1284, 1431, 1543, 3662
FspI	TGCGCA	1 cut(s)	3476
GlaI	GCGC	5 cut(s)	1367, 2367, 3476, 4367, 4953
GluI	GCNGC	9 cut(s)	284, 408, 411, 859, 1176, 1284, 1431, 1543, 3662
GsaI	CCCAGC	1 cut(s)	2181
GsuI	CTGGAG	9 cut(s)	294, 1683, 1869, 2235, 2466, 2598, 3569, 3927, 4496
HaeII	RGCGCY	3 cut(s)	1369, 2369, 4369
HaeIII	GGCC	9 cut(s)	47, 68, 1000, 1678, 2277, 2517, 2946, 3256, 3770
HgaI	GACGC	1 cut(s)	4337
HhaI	GCGC	5 cut(s)	1368, 2368, 3477, 4368, 4954
Hin6I	GCGC	5 cut(s)	1366, 2366, 3475, 4366, 4952
HinP1I	GCGC	5 cut(s)	1366, 2366, 3475, 4366, 4952
HincII	GTYRAC	4 cut(s)	2730, 4642, 4876, 5029
HindII	GTYRAC	4 cut(s)	2730, 4642, 4876, 5029
HindIII	AAGCTT	2 cut(s)	647, 4772
Hpy99I	CGWCG	2 cut(s)	4649, 5009
HpyCH4IV	ACGT	7 cut(s)	1067, 2953, 3208, 3307, 3379, 4519, 4647
HpyF10VI	GCNNNNNNNGC	9 cut(s)	289, 566, 1865, 2864, 3128, 3767, 3821, 3951, 5047
HpySE526I	ACGT	7 cut(s)	1067, 2953, 3208, 3307, 3379, 4519, 4647
HspAI	GCGC	5 cut(s)	1366, 2366, 3475, 4366, 4952
KflI	GGGWCCC	2 cut(s)	2174, 3781
Kpn2I	TCCGGA	2 cut(s)	2810, 3081
Ksp22I	TGATCA	1 cut(s)	5076
LguI	GCTCTTC	2 cut(s)	1341, 3858
Lsp1109I	GCAGC	8 cut(s)	394, 397, 870, 1162, 1295, 1442, 1529, 3648
LweI	GCATC	8 cut(s)	1645, 2744, 2854, 4018, 4140, 4335, 4714, 4727
MaeII	ACGT	7 cut(s)	1067, 2953, 3208, 3307, 3379, 4519, 4647
MfeI	CAATTG	3 cut(s)	1356, 2868, 3147
MflI	RGATCY	6 cut(s)	190, 835, 1333, 2813, 3535, 3668
MhlI	GDGCHC	3 cut(s)	3208, 3258, 3409
MlsI	TGGCCA	1 cut(s)	2277
MluNI	TGGCCA	1 cut(s)	2277
MmeI	TCCRAC	3 cut(s)	176, 2092, 4069
Mox20I	TGGCCA	1 cut(s)	2277
Mph1103I	ATGCAT	2 cut(s)	2942, 3697
MroI	TCCGGA	2 cut(s)	2810, 3081
MroXI	GAANNNNTTC	4 cut(s)	391, 1630, 2955, 3587
MscI	TGGCCA	1 cut(s)	2277
MslI	CAYNNNNRTG	3 cut(s)	2890, 2900, 3470
Msp20I	TGGCCA	1 cut(s)	2277
MspA1I	CMGCKG	1 cut(s)	407
MunI	CAATTG	3 cut(s)	1356, 2868, 3147
Mva1269I	GAATGC	2 cut(s)	3007, 4388
MvaI	CCWGG	7 cut(s)	1002, 1493, 1887, 2948, 2964, 3252, 4452
MvnI	CGCG	1 cut(s)	2559
MwoI	GCNNNNNNNGC	9 cut(s)	289, 566, 1865, 2864, 3128, 3767, 3821, 3951, 5047
NciI	CCSGG	4 cut(s)	502, 773, 1485, 4905
NdeI	CATATG	2 cut(s)	2641, 2740
NheI	GCTAGC	1 cut(s)	617
NsbI	TGCGCA	1 cut(s)	3476
NsiI	ATGCAT	2 cut(s)	2942, 3697
NspI	RCATGY	3 cut(s)	2940, 3450, 3699
NspV	TTCGAA	2 cut(s)	1634, 4308
OliI	CACNNNNGTG	1 cut(s)	2900
PagI	TCATGA	2 cut(s)	763, 2758
PasI	CCCWGGG	1 cut(s)	2963
PceI	AGGCCT	2 cut(s)	68, 2517
PciI	ACATGT	1 cut(s)	3446
PciSI	GCTCTTC	2 cut(s)	1341, 3858
PcsI	WCGNNNNNNNCGW	1 cut(s)	5013
PctI	GAATGC	2 cut(s)	3007, 4388
PdmI	GAANNNNTTC	4 cut(s)	391, 1630, 2955, 3587
Pfl23II	CGTACG	1 cut(s)	4516
PflFI	GACNNNGTC	1 cut(s)	359
PflMI	CCANNNNNTGG	1 cut(s)	2284
PinAI	ACCGGT	3 cut(s)	698, 1222, 2432
PkrI	GCNGC	9 cut(s)	285, 409, 412, 860, 1177, 1285, 1432, 1544, 3663
PmaCI	CACGTG	1 cut(s)	3209
PmlI	CACGTG	1 cut(s)	3209
Ppu21I	YACGTR	2 cut(s)	3209, 4520
PpuMI	RGGWCCY	3 cut(s)	2174, 2346, 3781
PscI	ACATGT	1 cut(s)	3446
PshBI	ATTAAT	2 cut(s)	1592, 3993
Psp5II	RGGWCCY	3 cut(s)	2174, 2346, 3781
Psp6I	CCWGG	7 cut(s)	1000, 1491, 1885, 2946, 2962, 3250, 4450
PspCI	CACGTG	1 cut(s)	3209
PspFI	CCCAGC	1 cut(s)	2177
PspGI	CCWGG	7 cut(s)	1000, 1491, 1885, 2946, 2962, 3250, 4450
PspLI	CGTACG	1 cut(s)	4516
PspOMI	GGGCCC	1 cut(s)	3254
PspPPI	RGGWCCY	3 cut(s)	2174, 2346, 3781
PstI	CTGCAG	2 cut(s)	340, 4843
PstNI	CAGNNNCTG	2 cut(s)	89, 410
PsuI	RGATCY	6 cut(s)	190, 835, 1333, 2813, 3535, 3668
PsyI	GACNNNGTC	1 cut(s)	359
PvuII	CAGCTG	1 cut(s)	407
RseI	CAYNNNNRTG	3 cut(s)	2890, 2900, 3470
SalI	GTCGAC	1 cut(s)	5027
SapI	GCTCTTC	2 cut(s)	1341, 3858
SatI	GCNGC	9 cut(s)	284, 408, 411, 859, 1176, 1284, 1431, 1543, 3662
ScaI	AGTACT	2 cut(s)	2134, 3744
SduI	GDGCHC	3 cut(s)	3208, 3258, 3409
SfaNI	GCATC	8 cut(s)	1645, 2744, 2854, 4018, 4140, 4335, 4714, 4727
SfcI	CTRYAG	6 cut(s)	336, 675, 1163, 2776, 4602, 4839
SfuI	TTCGAA	2 cut(s)	1634, 4308
SmiMI	CAYNNNNRTG	3 cut(s)	2890, 2900, 3470
SmlI	CTYRAG	5 cut(s)	863, 1229, 1993, 4853, 5126
SmoI	CTYRAG	5 cut(s)	863, 1229, 1993, 4853, 5126
SpeI	ACTAGT	1 cut(s)	683
SseBI	AGGCCT	2 cut(s)	68, 2517
SsiI	CCGC	5 cut(s)	284, 367, 567, 1946, 3533
SspI	AATATT	3 cut(s)	1843, 3041, 4314
StuI	AGGCCT	2 cut(s)	68, 2517
StyI	CCWWGG	1 cut(s)	2262
TaiI	ACGT	7 cut(s)	1070, 2956, 3211, 3310, 3382, 4522, 4650
TaqII	GACCGA	2 cut(s)	628, 3258
TatI	WGTACW	9 cut(s)	1558, 1718, 1965, 2101, 2132, 2470, 3742, 4245, 5105
TauI	GCSGC	1 cut(s)	286
TseI	GCWGC	8 cut(s)	407, 410, 858, 1175, 1283, 1430, 1542, 3661
TspGWI	ACGGA	2 cut(s)	1754, 4739
Tth111I	GACNNNGTC	1 cut(s)	359
Van91I	CCANNNNNTGG	1 cut(s)	2284
VspI	ATTAAT	2 cut(s)	1592, 3993
XagI	CCTNNNNNAGG	1 cut(s)	1728
XbaI	TCTAGA	1 cut(s)	4351
XceI	RCATGY	3 cut(s)	2940, 3450, 3699
XcmI	CCANNNNNNNNNTGG	1 cut(s)	184
XmiI	GTMKAC	8 cut(s)	171, 230, 929, 1671, 2012, 2252, 2495, 5028
XmnI	GAANNNNTTC	4 cut(s)	391, 1630, 2955, 3587
ZrmI	AGTACT	2 cut(s)	2134, 3744
Zsp2I	ATGCAT	2 cut(s)	2942, 3697

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.