Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr5

Orthogroup ID

OG0006854

Physical Location & Seq

Genomic Coordinates Forward (+)

26927088 .. 26938939

Gene Structure 11852 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom05g25590.9

Sequence Viewer

CDS Protein Promoter (2k)

Length: 5142 bp

ATGGAGGACAATTTAGTGGTGGGGGGCGAAGAGACGGTTGAAAAGGCCCCAATTTCGGAGAAACATGCCTCTGTTCGTTCATCAGAAACTGCCAAAGGAGTTACCAAAACTGTGAAAACCGGCAATGCAGTGACATCAAGGGTCTCGGTTCCAACTGTTAGAAAGAAAGTAGACCCCAAAAGTGGCGTGGATCCTAGTTCAAGGGCTACCAAGTCTAGTGCAAATGGGTCTACCCGAAGTTTGAATTCAGTCCCGATAAGGCGAAACAGCACTGGAGGTTTGCCGCAGAAGCCATCAGTCTCCACCGCAAGACAGCAAAACAATGCCACTACGACTGCAGTAAAAAAGACTCTGGATCCTGTCAGGCGGTCCCTCCCAGAGCTTAGAAGGAGTTCATTGCCTTCAGCTGCTACCACTAAATCTTTGACTAGAATCAGTGTTTCAGAAGTTAGAAAGTCGGTTTCAGGATCGCCATTGGACCGGAGTCTGAATAAATCATCCGGGTCAAATGTGAGTGTAACTAAACAAGAGACTGTCAGGAAGCCTGCTGTCAAACCTGCATTGTCTACTTCATCTTCCTCTTCACGAAGGGCGACTTCCTCATTGGATAGTAGTGCTAGCAGTGGAGTCAGGAAGTCGGTCCCAAAGCTTTCTTCCCCATCAGCTCGAACACCTACAGTTACTAGTGGGCTGAAAACCGGTTCTTTGTCCTCTTCTCTTGATAGAAGTTCTAGTTTATCTGGGCGTAGGAAAACTGCAACTCCTCAAAGCCGGGATTCTCGGTTCATTGTCCTTCCACAAGTGGAAGTCAAAGCCAGTGATGATGTGAGGTTAGATCTTAGGGGTCACAGAGTTCGCAGCCTCAAGGCTAGTGGGTTGAACTTGTCACCAAATTTAGAGTTCGTTTATCTCAGAGACAATCTCTTGTCTACATTGGAGGGAGTGGAAATACTGGCACGAGTAAAGGTTCTTGATTTGAGTTTCAATGATTTCAAAGGGCCTGGATTTGAACCTCTTGAAACTTGCAAAGTCTTACAGCAACTTTATCTTGCCGGAAACCAACTCACGTCACTTGCAAGCCTTCCTCAGCTCCCTAATTTGGAGTTTCTCTCTGTTGCTCAAAATAAATTGAAGTCTCTTACAATGGCAAGCCAACCTAGACTACAGGTATTAGCTGCCAGCAAGAACAAAATTTCAACTCTGAAAGGTTTCCCCTATTTACCAGTCCTTGAGCATTTACGAGTGGAGGAAAATCCCATACTTAAAATGCCTCACCTGGAAGCAGCTTCCATTTTGCTTGTTGGTACTACATTAAAAAAGTTCAATGACAGAGATCTTTCCCGAGAAGAGCTTACAATTGCAAAGCGCTATCCGGCACACACCTCTTTGTGCATTAGAGATGGTTGGGAGTTTTGCCATCCAGACCATGCTGCAGACTCTACTTTTCGTTTTCTTGTTGAGCAATGGAAGGATCATTTGCCTCCCGGCTTCCTGGTCAAGGAGGCCTCTGTTGAGAAACCATTTGAAGAAGATACTTGTCGCTGCCAGTTCACTTTTGTACAAGAGAACACTTTGGCAGCTGATCCTCAATTAACCTTGAAATACCAATGGTTTGTAGGAGAAAGAACCCCTTCGAATTTCACCATTATTCCTGATGCCACTGGAGAGGTCTACTGGCCTAAGCATGAAGATATTGGTAGAATTTTAAAAGTGGAGTGTACTCCTGTATTAGGAGAAACAGAGTACCCTTCTATTTTTGCTATATCTTCTCCAGTTAAACGAGGAAGTGGAATCCCAAAGGTTGTGAATCTTGATGTTCGTGGAAATCTAGTGGAGGGAAATATTGTTAGAGGTCATGCAAAGGTTGCGTGGTGTGGTGGCACTCCAGGGAAAGGTGTTTCTAGTTGGTTGAGGAGAAAATGGAATAGCAGTCCTGTGGTTATTGCAGGAGCTGAAGATGAGGAGTACAAATTGACCATAGATGATGTTGACTCCAGTTTGGTTTTCATGTATACACCAGTGACAGAAGAAGGTGCCAAAGGAGAACCTCATTATGAATACACCGATTTTGTAAAATCAGCCCCTCCATCAGTCAATAATGTACACATTGTTGGAGATGTTGTTGAAGGCGGTACTATCAGAGGAGTTGGTGATTACTTTGGAGGAAGAGAGGGTCCCAGCAAGTTTGAATGGTTATGTGAGGATAAAGACACTGGAGACCTTGTTTTGGTATCAATGGGTACACCTGAGTATACTTTGACCAAGGAAGATGTTGGCCATCGCTTGGCTTTTGTGTATATACCCATCAATTTGGAAGGTCAAGAAGGGGAATCTGCTTCGATAAGGTCCCATGTTGTGAAGCCAGCGCCTCCAAAGGTAATAGATTTGAAGATAGTTGGTGATCTAAGGGAGAACAGTAAGGTTACTGCAACTGGTACTGTCACTGGAGGAACTGAAGGGTCTAGCAGAGTACAGTGGTTTAAAACAAGTTCGTCTACTTTGGATGGTGAGAAAGGTCTTGAATCTTTAAGCACATCTAAGATTGCAAAGGCTTTTCGCGTACCCTTAGGAGCTGTTGGCTATTACATTGTTGTAAAATTTACTCCTATGACTCCAGATGGTGAATCTGGTGAACCAGCATATGCTATATCAGATAGAGCAGTTGAGACTTTGCCTCCAAGCCTAAATTTCTTATCAATTACTGGTGATTACACCGAAGGTGGAATGTTGACGGCATCATATGGTTACATTGGTGGTCATGAAGGAAAAAGTATCTATAGTTGGTATCTTCACGAGGTTGAAACTGACTCCGGATCTCTATTACCTGAGGTCACAGGGCTTCTTCAGTATCGTATAGCAAAAGATGCAATTGGTAAATTCATTTCATTCAAATGCACCCCTGTGCGTGATGATGGGATTGTGGGTGAGCTAAGAACATGCATGGGCCAGGAACGTGTTCGCCCTGGGAACCCTAGATTGCTTTCTCTACAAATCGTTGGGAATGCCACTGAGGGAACTGCACTTAGTGTAGAAAAGAAATATTGGGGCGGTGAAGAGGGGAATTCAGTTTTCTACTGGTTCCGGACTAATTCAGATGGAACACAAACCGAAATCAGGGGTGCAACAACTGCTTCATACACACTCTCAATTGATGATATTGGCTTCTTTGTCTCAGTATCTTGTGAGCCTGTCAGAAGTGACTGGGCGCGTGGTCCTACTGTTCTATCCGAACAAATTGGACCTGTAATACCTGGGCCCCCAACTTGTCGGTCTTTGGAGTTTCTTGGTTCAATGATAGAAGGGCAACGTCTGAGCTTTACTGCTTCTTATAGTGGAGGGGAGAAAGGAGATTGCTCGCACGAATGGTTTAGAGTTAAACGTAATGGTGTCAAGGAGAAGTTGAGTACTCAGGATTTTGTGGATTTGACTCTTGATGATGTGGGTACATGTGTTGAACTTGTTTACACTCCCATGCGCAAAGATGGGATGAGAGGAAATCCTAAGAGCATACAATCTGATGTGATTGCTCCTGCGGATCCAGTAGGACTGGAGCTTGTTATTTCCGATTGCTGTGAGAATGAGAATTTATTCCCCAAAAAAACATATTTTGGTGGAGAAGAAGGTGTTGGGGAATATATTTGGTACAGAACAAAGAATAAGCTGCATGGATCTGCATTGCAGGACATATATAATGCATGTGAAGATTTGGTTATTTGTGGTAAAACATTAACATACACACCAGTACTTGAAGATGTGGGGGCATATTTGGCCTTACATTGGGTCCCCACTCGTTCTGATGGCAAATGTGGACATGCACTAGTTGCCATTTGCAACTTTCCAGTTGCACCTGCTCTTCCTGTGGTTTCTAATGTTCGTGTGAAGGAGTTGTCACAAAGCGTTTATTCTGGAGAAGGAGAGTATTTTGGTGGATATGAAGGATCGAGCCTTTTTAGCTGGTATAGAGAAACTAATGAAGGAACTATTGTTCTTATTAATGGAGCCAATACTAACACTTATGAAGTCACTGATGCAGACTACAACTGCCGTCTATTGTTTGGATACACACCAGTTCGTTCAGATTCGGTGGTTGGAGAACTGAAGTTGTCTGAGCCAACTGATATTATTCTTCCAGAGCTTCCAAGACTAGAGATGCTTGCTCTCACGGGAAAGGCAATCGAAGGTGGTGTTCTTACAGTTGTTCAAGTGATCCCAGAGAGTGAGACTCAACAGATTGTTTGGAGCAAGTACAAGAAAGATGTCACATATCAATGGTATTTTTCTTCAAAAGAGGGAGATGAGAAAACCTTCGAATTATTACCTGCACAGAAATCTTGCTCCTACAAGATGCGTCTAGAAGATGTTGGGCGCTGTCTAAAATGCGAATGCGTTGTGACTGATGTCTTTGGAAGATCAACTGAGCCTGTGTATGCTGAAACGGGACCCATATTACCAGGAATCCCTAGAATAGATAAGCTGGAGATTGAAGGGAGGGGCTTCCACACCAACTTATACGCCGTACGTGGAATTTATAGTGGAGGGAAGGAAGGAAAAAGTAGAATCCAGTGGCTCAGATCAATGGTTGGCAGTCCTGATCTTATCTCTATACCAGGGGAGGTAGGGCGAATGTACGAGTCTAATGTTGACGACGTAGGGTACAGGCTTGTTGCCATTTACACTCCAGTAAGAGAGGATGGAGTGGAGGGGCAACCTGTTTCTGCATCAACGGAGCCGATTGCAGTTGAACCTGATGTTCTTAAAGAAGTGAAGCAGAAGCTTGATCTTGGAACTGTGAAATTTGAGACATTGTGTGACAAGGACCAATCTACGAAAAAGGCTCCTGCAGTGGGAAGTCTTGAGAGAAGAATCCTCGAAGTCAACAGAAAGAGAGTCAAGGTGGTAAAGCCCGGTTCTAAGACTTCCTTCCCAACCACTGAAATTCGTGGAAGTTACGCGCCTCCCTTTCATGTGGAGCTGTTCCGAAACGACCAACACCGTCTGAAAATCGTCGTGGACGGCGAGAATAATGTCGACTTGATGGTGCAGTCAAGGCACCTTCGGGATGTAACTGTGCTGGTGATCAGAGGGCTTGCTCAGAGATTCAACAGTACATCACTCAATACCCTCCTCAAGATAGAGGCTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000226 GO:0001578 GO:0001669 GO:0002177 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005856 GO:0006996 GO:0007010 GO:0007017 GO:0007276 GO:0007283 GO:0008150 GO:0009987 GO:0012505 GO:0015630 GO:0016043 GO:0019953 GO:0022414 GO:0022607 GO:0030030 GO:0030031 GO:0030141 GO:0031410 GO:0031982 GO:0032501 GO:0032504 GO:0035082 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043232 GO:0044085 GO:0044422 GO:0044424 GO:0044430 GO:0044444 GO:0044446 GO:0044464 GO:0044703 GO:0044782 GO:0048232 GO:0048609 GO:0051704 GO:0060271 GO:0070925 GO:0071840 GO:0097223 GO:0097708 GO:0099503 GO:0120031 GO:0120036

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

LRR_4

Protein Analysis

1714

Amino Acids

187.56

Weight (kDa)

6.82

Isoelectric Point (pI)

37.88

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006854)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G34680 AT2G34680
fragaria_vesca	FvH4_3g09950 FvH4_3g09950 FvH4_3g09950 FvH4_3g09950 FvH4_3g09950
malus_domestica	MD05G1274500.v1.1 MD10G1254200.v1.1
prunus_persica	Prupe.4G089200_v2.0.a1 Prupe.4G089200_v2.0.a1 Prupe.4G089200_v2.0.a1
pyrus_communis	pycom05g25590 pycom10g20990
rosa_chinensis	RchiOBHm_Chr5g0015701
rosa_laevigata	RLG00000032220
rosa_multiflora	Rmu_sc0000843.1_g000009
rosa_roxburghii	Rroxscaffold_1G00060820
rosa_rugosa	Rorug05G0029200 Rorug05G0029200 Rorug05G0029300
rosa_samantha	Rh5AG122500 Rh5BG121800 Rh5CG132000 Rh5DG120500
rosa_wichuraiana	Rw5G010580

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	3856
Acc16I	TGCGCA	1 cut(s)	3476
Acc36I	ACCTGC	3 cut(s)	565, 3856, 4327
AccB1I	GGYRCC	2 cut(s)	2033, 5049
AccB7I	CCANNNNNTGG	1 cut(s)	2284
AccI	GTMKAC	9 cut(s)	171, 230, 566, 929, 1671, 2012, 2252, 2495, 5028
AccII	CGCG	3 cut(s)	2559, 3208, 4952
AccIII	TCCGGA	2 cut(s)	2810, 3081
AciI	CCGC	6 cut(s)	284, 306, 367, 2130, 3048, 3533
AcoI	YGGCCR	1 cut(s)	2275
AcuI	CTGAAG	5 cut(s)	387, 1974, 2475, 2828, 4118
AdeI	CACNNNGTG	1 cut(s)	3026
AfeI	AGCGCT	1 cut(s)	1367
AflIII	ACRYGT	2 cut(s)	2953, 3446
AgeI	ACCGGT	1 cut(s)	698
AhlI	ACTAGT	2 cut(s)	683, 3817
AjiI	CACGTC	1 cut(s)	1068
AjnI	CCWGG	9 cut(s)	1000, 1275, 1491, 1885, 2946, 2962, 3250, 4450, 4606
AjuI	GAANNNNNNNTTGG	4 cut(s)	637, 669, 3609, 3641
AleI	CACNNNNGTG	1 cut(s)	2900
AloI	GAACNNNNNNTCC	4 cut(s)	767, 799, 3969, 4001
AlwNI	CAGNNNCTG	2 cut(s)	89, 1952
Ama87I	CYCGRG	1 cut(s)	1341
Aor13HI	TCCGGA	2 cut(s)	2810, 3081
Aor51HI	AGCGCT	1 cut(s)	1367
AoxI	GGCC	8 cut(s)	45, 998, 1503, 1676, 2275, 2944, 3254, 3768
ApaI	GGGCCC	1 cut(s)	3258
ApeKI	GCWGC	8 cut(s)	407, 858, 1175, 1283, 1430, 1542, 1577, 3661
AseI	ATTAAT	1 cut(s)	3993
AsiGI	ACCGGT	1 cut(s)	698
Asp700I	GAANNNNTTC	6 cut(s)	391, 1208, 1630, 2955, 3587, 4304
AspLEI	GCGC	6 cut(s)	1368, 2368, 3208, 3477, 4368, 4954
AsuC2I	CCSGG	4 cut(s)	502, 773, 1485, 4905
AsuII	TTCGAA	2 cut(s)	1634, 4308
AsuNHI	GCTAGC	1 cut(s)	617
AvaI	CYCGRG	1 cut(s)	1341
AxyI	CCTNAGG	2 cut(s)	2566, 2826
BaeGI	GKGCMC	1 cut(s)	3258
BaeI	ACNNNNGTAYC	2 cut(s)	3435, 3468
BalI	TGGCCA	1 cut(s)	2277
BamHI	GGATCC	3 cut(s)	190, 355, 3535
BanI	GGYRCC	2 cut(s)	2033, 5049
BanII	GRGCYC	1 cut(s)	3258
BauI	CACGAG	2 cut(s)	957, 2792
BbvCI	CCTCAGC	1 cut(s)	1086
BbvI	GCAGC	8 cut(s)	394, 870, 1162, 1295, 1417, 1529, 1589, 3648
BceAI	ACGGC	4 cut(s)	2748, 4029, 4499, 5029
BciT130I	CCWGG	9 cut(s)	1002, 1277, 1493, 1887, 2948, 2964, 3252, 4452, 4608
BciVI	GTATCC	1 cut(s)	4052
BclI	TGATCA	1 cut(s)	5076
BcnI	CCSGG	4 cut(s)	502, 773, 1485, 4905
BcuI	ACTAGT	2 cut(s)	683, 3817
BfmI	CTRYAG	6 cut(s)	336, 675, 1163, 1431, 2776, 4839
BfoI	RGCGCY	3 cut(s)	1369, 2369, 4369
BfuAI	ACCTGC	3 cut(s)	565, 3856, 4327
BfuI	GTATCC	1 cut(s)	4052
BglII	AGATCT	2 cut(s)	835, 1333
BisI	GCNGC	9 cut(s)	284, 408, 859, 1176, 1284, 1431, 1543, 1578, 3662
BlsI	GCNGC	9 cut(s)	285, 409, 860, 1177, 1285, 1432, 1544, 1579, 3663
BmcAI	AGTACT	2 cut(s)	3406, 3744
BmeT110I	CYCGRG	1 cut(s)	1341
BmgBI	CACGTC	1 cut(s)	1068
BmrI	ACTGGG	1 cut(s)	3211
BmsI	GCATC	7 cut(s)	1645, 2744, 2854, 4018, 4140, 4335, 4727
BmtI	GCTAGC	1 cut(s)	621
BmuI	ACTGGG	1 cut(s)	3211
BoxI	GACNNNNGTC	2 cut(s)	483, 4397
BplI	GAGNNNNNCTC	6 cut(s)	906, 938, 1094, 1126, 4207, 4239
Bpu10I	CCTNAGC	2 cut(s)	1086, 1680
Bpu14I	TTCGAA	2 cut(s)	1634, 4308
BpuEI	CTTGAG	4 cut(s)	848, 1250, 4874, 5111
BpuMI	CCSGG	4 cut(s)	502, 773, 1485, 4905
BsaAI	YACGTR	1 cut(s)	4520
BsaI	GGTCTC	2 cut(s)	148, 2211
BsaJI	CCNNGG	6 cut(s)	1886, 2262, 2962, 2963, 3251, 4607
BsaWI	WCCGGW	4 cut(s)	480, 698, 2810, 3081
BsaXI	ACNNNNNCTCC	8 cut(s)	745, 775, 2106, 2136, 2208, 2238, 2659, 2689
Bse118I	RCCGGY	2 cut(s)	119, 698
Bse21I	CCTNAGG	2 cut(s)	2566, 2826
Bse3DI	GCAATG	4 cut(s)	130, 395, 1469, 3674
BseAI	TCCGGA	2 cut(s)	2810, 3081
BseBI	CCWGG	9 cut(s)	1002, 1277, 1493, 1887, 2948, 2964, 3252, 4452, 4608
BseDI	CCNNGG	6 cut(s)	1886, 2262, 2962, 2963, 3251, 4607
BseGI	GGATG	6 cut(s)	497, 1417, 2509, 3492, 4696, 5065
BseMI	GCAATG	4 cut(s)	130, 395, 1469, 3674
BseRI	GAGGAG	5 cut(s)	753, 1927, 1976, 2157, 5114
BseSI	GKGCMC	1 cut(s)	3258
BseXI	GCAGC	8 cut(s)	394, 870, 1162, 1295, 1417, 1529, 1589, 3648
BseYI	CCCAGC	1 cut(s)	2177
BsgI	GTGCAG	3 cut(s)	3003, 4305, 5060
Bsh1236I	CGCG	3 cut(s)	2559, 3208, 4952
BshFI	GGCC	8 cut(s)	47, 1000, 1505, 1678, 2277, 2946, 3256, 3770
BshNI	GGYRCC	2 cut(s)	2033, 5049
BshTI	ACCGGT	1 cut(s)	698
BsiHKCI	CYCGRG	1 cut(s)	1341
BsiWI	CGTACG	1 cut(s)	4516
BslFI	GGGAC	7 cut(s)	236, 355, 626, 2160, 2332, 3767, 4452
BsmBI	CGTCTC	1 cut(s)	26
BsmFI	GGGAC	7 cut(s)	236, 355, 626, 2160, 2332, 3767, 4452
BsmI	GAATGC	2 cut(s)	3007, 4388
BsnI	GGCC	8 cut(s)	47, 1000, 1505, 1678, 2277, 2946, 3256, 3770
Bso31I	GGTCTC	2 cut(s)	148, 2211
BsoBI	CYCGRG	1 cut(s)	1341
Bsp119I	TTCGAA	2 cut(s)	1634, 4308
Bsp120I	GGGCCC	1 cut(s)	3254
Bsp1286I	GDGCHC	1 cut(s)	3258
Bsp13I	TCCGGA	2 cut(s)	2810, 3081
Bsp1407I	TGTACA	2 cut(s)	1558, 2101
BspACI	CCGC	6 cut(s)	284, 306, 367, 2130, 3048, 3533
BspANI	GGCC	8 cut(s)	47, 1000, 1505, 1678, 2277, 2946, 3256, 3770
BspEI	TCCGGA	2 cut(s)	2810, 3081
BspFNI	CGCG	3 cut(s)	2559, 3208, 4952
BspHI	TCATGA	1 cut(s)	2758
BspMAI	CTGCAG	3 cut(s)	340, 1435, 4843
BspMI	ACCTGC	3 cut(s)	565, 3856, 4327
BspOI	GCTAGC	1 cut(s)	621
BspQI	GCTCTTC	2 cut(s)	1341, 3858
BspT104I	TTCGAA	2 cut(s)	1634, 4308
BspT107I	GGYRCC	2 cut(s)	2033, 5049
BspTNI	GGTCTC	2 cut(s)	148, 2211
BsrDI	GCAATG	4 cut(s)	130, 395, 1469, 3674
BsrFI	RCCGGY	2 cut(s)	119, 698
BsrGI	TGTACA	2 cut(s)	1558, 2101
BssAI	RCCGGY	2 cut(s)	119, 698
BssECI	CCNNGG	6 cut(s)	1886, 2262, 2962, 2963, 3251, 4607
BssNAI	GTATAC	2 cut(s)	2013, 2253
BssSI	CACGAG	2 cut(s)	957, 2792
BssT1I	CCWWGG	1 cut(s)	2262
Bst1107I	GTATAC	2 cut(s)	2013, 2253
Bst2BI	CACGAG	2 cut(s)	957, 2792
Bst2UI	CCWGG	9 cut(s)	1002, 1277, 1493, 1887, 2948, 2964, 3252, 4452, 4608
Bst6I	CTCTTC	7 cut(s)	24, 586, 718, 1341, 2161, 3048, 3858
BstAPI	GCANNNNNTGC	4 cut(s)	1865, 2864, 3128, 3821
BstAUI	TGTACA	2 cut(s)	1558, 2101
BstBAI	YACGTR	1 cut(s)	4520
BstBI	TTCGAA	2 cut(s)	1634, 4308
BstC8I	GCNNGC	9 cut(s)	546, 619, 1078, 1150, 1180, 2364, 3356, 4155, 5088
BstENI	CCTNNNNNAGG	2 cut(s)	1496, 1728
BstF5I	GGATG	6 cut(s)	497, 1417, 2509, 3492, 4696, 5065
BstFNI	CGCG	3 cut(s)	2559, 3208, 4952
BstH2I	RGCGCY	3 cut(s)	1369, 2369, 4369
BstHHI	GCGC	6 cut(s)	1368, 2368, 3208, 3477, 4368, 4954
BstMWI	GCNNNNNNNGC	8 cut(s)	289, 1865, 2864, 3128, 3767, 3821, 3951, 5047
BstNI	CCWGG	9 cut(s)	1002, 1277, 1493, 1887, 2948, 2964, 3252, 4452, 4608
BstNSI	RCATGY	5 cut(s)	68, 2940, 3450, 3699, 3815
BstPAI	GACNNNNGTC	2 cut(s)	483, 4397
BstSFI	CTRYAG	6 cut(s)	336, 675, 1163, 1431, 2776, 4839
BstSLI	GKGCMC	1 cut(s)	3258
BstUI	CGCG	3 cut(s)	2559, 3208, 4952
BstV1I	GCAGC	8 cut(s)	394, 870, 1162, 1295, 1417, 1529, 1589, 3648
BstX2I	RGATCY	7 cut(s)	190, 355, 835, 1333, 2813, 3535, 3668
BstXI	CCANNNNNNTGG	1 cut(s)	2311
BstYI	RGATCY	7 cut(s)	190, 355, 835, 1333, 2813, 3535, 3668
BstZ17I	GTATAC	2 cut(s)	2013, 2253
Bsu36I	CCTNAGG	2 cut(s)	2566, 2826
BsuI	GTATCC	1 cut(s)	4052
BsuRI	GGCC	8 cut(s)	47, 1000, 1505, 1678, 2277, 2946, 3256, 3770
BtgZI	GCGATG	1 cut(s)	2264
BtrI	CACGTC	1 cut(s)	1068
BtsCI	GGATG	6 cut(s)	497, 1417, 2509, 3492, 4696, 5065
BtsI	GCAGTG	3 cut(s)	135, 628, 4848
BveI	ACCTGC	3 cut(s)	565, 3856, 4327
Cac8I	GCNNGC	9 cut(s)	546, 619, 1078, 1150, 1180, 2364, 3356, 4155, 5088
CaiI	CAGNNNCTG	2 cut(s)	89, 1952
CciI	TCATGA	1 cut(s)	2758
CfoI	GCGC	6 cut(s)	1368, 2368, 3208, 3477, 4368, 4954
Cfr10I	RCCGGY	2 cut(s)	119, 698
CseI	GACGC	1 cut(s)	4337
CspAI	ACCGGT	1 cut(s)	698
CspCI	CAANNNNNGTGG	2 cut(s)	853, 888
DraI	TTTAAA	2 cut(s)	1707, 2482
DraIII	CACNNNGTG	1 cut(s)	3026
EaeI	YGGCCR	1 cut(s)	2275
Eam1104I	CTCTTC	7 cut(s)	24, 586, 718, 1341, 2161, 3048, 3858
EarI	CTCTTC	7 cut(s)	24, 586, 718, 1341, 2161, 3048, 3858
Eco130I	CCWWGG	1 cut(s)	2262
Eco147I	AGGCCT	1 cut(s)	1505
Eco24I	GRGCYC	1 cut(s)	3258
Eco31I	GGTCTC	2 cut(s)	148, 2211
Eco47III	AGCGCT	1 cut(s)	1367
Eco57I	CTGAAG	5 cut(s)	387, 1974, 2475, 2828, 4118
Eco81I	CCTNAGG	2 cut(s)	2566, 2826
Eco88I	CYCGRG	1 cut(s)	1341
EcoNI	CCTNNNNNAGG	2 cut(s)	1496, 1728
EcoO109I	RGGNCCY	7 cut(s)	46, 998, 2174, 2346, 3255, 3781, 4439
EcoRI	GAATTC	2 cut(s)	244, 3061
EcoRII	CCWGG	9 cut(s)	1000, 1275, 1491, 1885, 2946, 2962, 3250, 4450, 4606
EcoT14I	CCWWGG	1 cut(s)	2262
EcoT22I	ATGCAT	2 cut(s)	2942, 3697
EcoT38I	GRGCYC	1 cut(s)	3258
ErhI	CCWWGG	1 cut(s)	2262
Esp3I	CGTCTC	1 cut(s)	26
FalI	AAGNNNNNCTT	6 cut(s)	580, 612, 1615, 1647, 4904, 4936
FaqI	GGGAC	7 cut(s)	236, 355, 626, 2160, 2332, 3767, 4452
FauNDI	CATATG	2 cut(s)	2641, 2740
FbaI	TGATCA	1 cut(s)	5076
FblI	GTMKAC	9 cut(s)	171, 230, 566, 929, 1671, 2012, 2252, 2495, 5028
Fnu4HI	GCNGC	9 cut(s)	284, 408, 859, 1176, 1284, 1431, 1543, 1578, 3662
FokI	GGATG	6 cut(s)	484, 1404, 2516, 3499, 4703, 5072
FriOI	GRGCYC	1 cut(s)	3258
Fsp4HI	GCNGC	9 cut(s)	284, 408, 859, 1176, 1284, 1431, 1543, 1578, 3662
FspI	TGCGCA	1 cut(s)	3476
GlaI	GCGC	6 cut(s)	1367, 2367, 3207, 3476, 4367, 4953
GluI	GCNGC	9 cut(s)	284, 408, 859, 1176, 1284, 1431, 1543, 1578, 3662
GsaI	CCCAGC	1 cut(s)	2181
HaeII	RGCGCY	3 cut(s)	1369, 2369, 4369
HaeIII	GGCC	8 cut(s)	47, 1000, 1505, 1678, 2277, 2946, 3256, 3770
HgaI	GACGC	1 cut(s)	4337
HhaI	GCGC	6 cut(s)	1368, 2368, 3208, 3477, 4368, 4954
Hin6I	GCGC	6 cut(s)	1366, 2366, 3206, 3475, 4366, 4952
HinP1I	GCGC	6 cut(s)	1366, 2366, 3206, 3475, 4366, 4952
HincII	GTYRAC	5 cut(s)	1990, 2730, 4642, 4876, 5029
HindII	GTYRAC	5 cut(s)	1990, 2730, 4642, 4876, 5029
HindIII	AAGCTT	2 cut(s)	647, 4772
Hpy99I	CGWCG	2 cut(s)	4649, 5009
HpyCH4IV	ACGT	6 cut(s)	1067, 2953, 3307, 3379, 4519, 4647
HpyF10VI	GCNNNNNNNGC	8 cut(s)	289, 1865, 2864, 3128, 3767, 3821, 3951, 5047
HpySE526I	ACGT	6 cut(s)	1067, 2953, 3307, 3379, 4519, 4647
HspAI	GCGC	6 cut(s)	1366, 2366, 3206, 3475, 4366, 4952
KflI	GGGWCCC	3 cut(s)	2174, 3781, 4439
Kpn2I	TCCGGA	2 cut(s)	2810, 3081
Ksp22I	TGATCA	1 cut(s)	5076
LguI	GCTCTTC	2 cut(s)	1341, 3858
Lsp1109I	GCAGC	8 cut(s)	394, 870, 1162, 1295, 1417, 1529, 1589, 3648
LweI	GCATC	7 cut(s)	1645, 2744, 2854, 4018, 4140, 4335, 4727
MaeII	ACGT	6 cut(s)	1067, 2953, 3307, 3379, 4519, 4647
MfeI	CAATTG	3 cut(s)	1356, 2868, 3147
MflI	RGATCY	7 cut(s)	190, 355, 835, 1333, 2813, 3535, 3668
MhlI	GDGCHC	1 cut(s)	3258
MlsI	TGGCCA	1 cut(s)	2277
MluNI	TGGCCA	1 cut(s)	2277
MmeI	TCCRAC	3 cut(s)	176, 2092, 4069
Mox20I	TGGCCA	1 cut(s)	2277
Mph1103I	ATGCAT	2 cut(s)	2942, 3697
MroI	TCCGGA	2 cut(s)	2810, 3081
MroXI	GAANNNNTTC	6 cut(s)	391, 1208, 1630, 2955, 3587, 4304
MscI	TGGCCA	1 cut(s)	2277
MslI	CAYNNNNRTG	4 cut(s)	2890, 2900, 3470, 4267
Msp20I	TGGCCA	1 cut(s)	2277
MspA1I	CMGCKG	2 cut(s)	407, 1580
MunI	CAATTG	3 cut(s)	1356, 2868, 3147
Mva1269I	GAATGC	2 cut(s)	3007, 4388
MvaI	CCWGG	9 cut(s)	1002, 1277, 1493, 1887, 2948, 2964, 3252, 4452, 4608
MvnI	CGCG	3 cut(s)	2559, 3208, 4952
MwoI	GCNNNNNNNGC	8 cut(s)	289, 1865, 2864, 3128, 3767, 3821, 3951, 5047
NciI	CCSGG	4 cut(s)	502, 773, 1485, 4905
NdeI	CATATG	2 cut(s)	2641, 2740
NheI	GCTAGC	1 cut(s)	617
NsbI	TGCGCA	1 cut(s)	3476
NsiI	ATGCAT	2 cut(s)	2942, 3697
NspI	RCATGY	5 cut(s)	68, 2940, 3450, 3699, 3815
NspV	TTCGAA	2 cut(s)	1634, 4308
OliI	CACNNNNGTG	1 cut(s)	2900
PagI	TCATGA	1 cut(s)	2758
PaqCI	CACCTGC	1 cut(s)	3856
PasI	CCCWGGG	1 cut(s)	2963
PceI	AGGCCT	1 cut(s)	1505
PciI	ACATGT	1 cut(s)	3446
PciSI	GCTCTTC	2 cut(s)	1341, 3858
PcsI	WCGNNNNNNNCGW	1 cut(s)	5013
PctI	GAATGC	2 cut(s)	3007, 4388
PdmI	GAANNNNTTC	6 cut(s)	391, 1208, 1630, 2955, 3587, 4304
Pfl23II	CGTACG	1 cut(s)	4516
PflMI	CCANNNNNTGG	1 cut(s)	2284
PinAI	ACCGGT	1 cut(s)	698
PkrI	GCNGC	9 cut(s)	285, 409, 860, 1177, 1285, 1432, 1544, 1579, 3663
Ppu21I	YACGTR	1 cut(s)	4520
PpuMI	RGGWCCY	4 cut(s)	2174, 2346, 3781, 4439
PscI	ACATGT	1 cut(s)	3446
PshAI	GACNNNNGTC	2 cut(s)	483, 4397
PshBI	ATTAAT	1 cut(s)	3993
Psp5II	RGGWCCY	4 cut(s)	2174, 2346, 3781, 4439
Psp6I	CCWGG	9 cut(s)	1000, 1275, 1491, 1885, 2946, 2962, 3250, 4450, 4606
PspFI	CCCAGC	1 cut(s)	2177
PspGI	CCWGG	9 cut(s)	1000, 1275, 1491, 1885, 2946, 2962, 3250, 4450, 4606
PspLI	CGTACG	1 cut(s)	4516
PspOMI	GGGCCC	1 cut(s)	3254
PspPPI	RGGWCCY	4 cut(s)	2174, 2346, 3781, 4439
PstI	CTGCAG	3 cut(s)	340, 1435, 4843
PstNI	CAGNNNCTG	2 cut(s)	89, 1952
PsuI	RGATCY	7 cut(s)	190, 355, 835, 1333, 2813, 3535, 3668
PvuII	CAGCTG	2 cut(s)	407, 1580
RseI	CAYNNNNRTG	4 cut(s)	2890, 2900, 3470, 4267
SalI	GTCGAC	1 cut(s)	5027
SapI	GCTCTTC	2 cut(s)	1341, 3858
SatI	GCNGC	9 cut(s)	284, 408, 859, 1176, 1284, 1431, 1543, 1578, 3662
ScaI	AGTACT	2 cut(s)	3406, 3744
SduI	GDGCHC	1 cut(s)	3258
SfaNI	GCATC	7 cut(s)	1645, 2744, 2854, 4018, 4140, 4335, 4727
SfcI	CTRYAG	6 cut(s)	336, 675, 1163, 1431, 2776, 4839
SfuI	TTCGAA	2 cut(s)	1634, 4308
SmiMI	CAYNNNNRTG	4 cut(s)	2890, 2900, 3470, 4267
SmlI	CTYRAG	4 cut(s)	863, 1229, 4853, 5126
SmoI	CTYRAG	4 cut(s)	863, 1229, 4853, 5126
SpeI	ACTAGT	2 cut(s)	683, 3817
SseBI	AGGCCT	1 cut(s)	1505
SsiI	CCGC	6 cut(s)	284, 306, 367, 2130, 3048, 3533
SspI	AATATT	2 cut(s)	1843, 3041
StuI	AGGCCT	1 cut(s)	1505
StyI	CCWWGG	1 cut(s)	2262
TaiI	ACGT	6 cut(s)	1070, 2956, 3310, 3382, 4522, 4650
TaqI	TCGA	8 cut(s)	667, 1634, 2339, 3941, 4176, 4308, 4869, 5028
TaqII	GACCGA	2 cut(s)	628, 3258
TatI	WGTACW	9 cut(s)	1558, 1718, 1965, 2101, 2470, 3404, 3742, 4245, 5105
TauI	GCSGC	1 cut(s)	286
TseI	GCWGC	8 cut(s)	407, 858, 1175, 1283, 1430, 1542, 1577, 3661
TspGWI	ACGGA	1 cut(s)	4739
Van91I	CCANNNNNTGG	1 cut(s)	2284
VspI	ATTAAT	1 cut(s)	3993
XagI	CCTNNNNNAGG	2 cut(s)	1496, 1728
XbaI	TCTAGA	1 cut(s)	4351
XceI	RCATGY	5 cut(s)	68, 2940, 3450, 3699, 3815
XcmI	CCANNNNNNNNNTGG	1 cut(s)	184
XmiI	GTMKAC	9 cut(s)	171, 230, 566, 929, 1671, 2012, 2252, 2495, 5028
XmnI	GAANNNNTTC	6 cut(s)	391, 1208, 1630, 2955, 3587, 4304
ZrmI	AGTACT	2 cut(s)	3406, 3744
Zsp2I	ATGCAT	2 cut(s)	2942, 3697

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.