Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr5D

Orthogroup ID

OG0006854

Physical Location & Seq

Genomic Coordinates Reverse (-)

10884149 .. 10901797

Gene Structure 17649 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh5DG120500.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4797 bp

ATGGAGGACAATGTAGAGCAACCAGGCAAAGACCCAGTTGAGAGTCCCCAAGTTTCTGAGAAGCAGGCCTCTGTTGGTTCATCAGAAACTGCAAAAAGAGTTACCAAGACAGTGAAACCTGGGGCTTCAGTGACATCCAAGGCCTCGGGTCCCATTAGTAGTGTTAGAAAGAAAGTAGAGGCGAAAAGTGGTTTGGATTCTAGCTCGAGTGCAACGAAGTCAAGTGCAACTGGGACTTCCAGAAGTTTGAATTCAGTTCCAGTAGCAAGGCGAAACAGCACTGGTGGTTTGCCTCAGAAGCCGTCAGTCTCCACTGCAAGACAGCAGAGCAATGCCACTCCAATTTCTGGCAATAAGTCTGCAGACCCTGTAAGGCGGTCTCTCCCCGAGCTTAGGAGAAGTTCTTTGCCTTCAGTAGTTACAAAATCAACCAGTGTTTCACAGGCTGTAAAGTTGTCTGCCGGGTCACCATTGGACCGGAGTCTGAATAAACCATCGGGTTCTCTTGCAAGTAGACAAGAGACTGTCAGGAAGCCTTCTGTCAAGCCAGCATCGTCCGTTTCATCTTCGTCTTCAAGGATGACAACTTCTTCATTGGACAGTAGTGCTAGTAGTGGAGTCAGGAAGTCAGTCTCAAAGCTTTCTTCCTCATCAGCTCGATCACCTACAGTTACCAGTGGACTGAGATCCGGGTCTTTGTCTTCGTCTATGAATAGTAGTGCTAGTTTATCTGGACGCAGGAAAGCGGCAACACCTCAAGGCCGAGATTCTCGGTTTATTGTTCTTCCAAAGGTGGAAATCAAAGCTGGTGATGATCTGAGGTTGGACCTTAGGGGTCACAGAGTTCGCAGTCTCAAGGCCAGTGGGTTGAACTTGTCACCAAATTTAGAGTTTGTTTATCTCAGAGATAATCTTTTATCTACGTTGGAAGGGGTTGAAATACTGACGAGAGTAAAGGTTCTTGATTTGAGTTTCAATGATTTCAAAGGGCCTGGATTTGAACCTCTTGAAAATTGCAGAGTCTTACAGCAACTTTATCTGGCTGGAAATCAAATCACGTCGCTTGCAAACCTCCCTCAGCTCCCCAATTTGGAGTTCCTCTCTGTTGCTCAAAATAAGTTGAAGAATCTTACAATGGCAAGTCAACCTCGTCTACAGGTATTAGCTGCCAGCAAAAACAAAATTTCAACTCTAAAGGGATTCCCGTATTTACCGGTTCTTGAGCATTTACGAGTGGAGGAGAATCCTATACTAAAAATGCCTCATGTAGAAGCAGCTTCCATTTTGCTTGCTGGTCCTACCTTAAAAAAGTTCAATGACAGAGATCTTTCCCGAGAACAGGTTGCAATTGCAAAGCGTTATCCTGCACACACATCATTATGCATTAGAGATGGTTGGGAGTTTTGCCGTCCAGAGCATGCAGCAGACTCAACGTTTCGCTTTCTAGTTGAGCAATGGAAGGATAACTTGCCTCCGGGCTTCCTGGTCAAGGAGGCCTTTATTGATCAGCCATTTGAAGAAGATACTTGTCGCTGTCACTTCTCTTTTGTACAAGACAGCACTTTGGTCACTGATCCACAATTAATCTTAAAATATCAGTGGTTTGTAGGAGAAAGAACACCTTCCAATTTCAACAGCATTCCTGATGCCACTGGAGAGGTATACTGGCCTAAGCATGAAGATATTGGTAAAATTTTGAAAGTGGAATGTACTCCAATATTAGGAGAAATGGAGTACGCTCCTATTTTTGCTATATCTTCATCAGTTTCACCAGGTACTGGAATTCCCAAGGTTGTGAACCTTGATGTTCGTGGAGAACTGGTGGAGGGAAATATTATAAGGGGCCATGCAGAGATTGCGTGGTGTGGCGGCACTCCAGCAAAAGGTGTTTCTAGTTGGTTGAGGAGGAAATGGAACAGCAGTCCTGTGGTTATTGCTGGAGCTGAAGATGAGGAGTATAAGTTGACCATAGATGATATCGATACAAGTTTGGTTTTCATGTATACACCAGTGACAGAAGAAGGTGCCAAAGGAGAACCCCATTATAAATACACCGATTTTGTAAAATCAGCACCGCCGTCGGTCAATAACGTTCAAATCGTCGGAGATCTTGTTGAAGGAACTACCATCAGAGGAGTTGGTGATTACTTTGGAGGAAGAGAGGGCCCTAGCAAATTTGAATGGTTATGTGAGCATAGCGACAGTGGAAACTTTGTTTTGGTATCAACTGGTACATCTGAGTATACTTTGAGCAAGGAGGATGTTGGGCATCGCTTGGCTTTTGCATATATACCTATCAATTTTGAAGGGCAAGAAGGGGAATCTGTTTCAATACTGTCCGATGTGGTGAAGCAAGCACCTCCGAAGGTATTAAATCTGAAGATAATAGGTGATATGAAGGAGAACAGTAAGGTTACTGCCACTGGTATTGTCACTGGAGGAACTGAAGGTTCTAGCAGGGTACAGTGGTTTAAAACAAGGTTCTCAACTTTGGAGGGTGAGAAAGGTCTTGAAGCGTTGAGCACATCCAAGATTGCCAAGGCGTTTCGCATACCATTGGGAGCTGTTGGCTATTACATTGTTGCAAAATTCACTCCTATGACTCCAGATGGTGAATCTGGTGACCCAGCATATGTTATTTCAGATACAGCAGTTGAGACTCTGCCTCCTAGCCTGAATTTCTTATCTATTACTGGAGATTACTCGGAAGGTGGAATATTGACTGGATCATATGGTTATATCGGTGGTCATGAAGGAAAAAGTCTCTATAATTGGTATATTCATGAGGTTGAAACTGATTCCGGATCTCTAATACCTGAGGTCACTGGGCTTCTTCAGTATCGTATTACAAAAGATGCAATTGGTAAATTCATTTCATTCCAATGTACCCCAGTGCGTGATGATGGGATTGTGGGTGAACCAACAACGTGCATGGGCCAGGAACGTGTTCGCCCTGGAAGCCCTAGATTGCTTTCCCTACGAATTGTCGGGGATGCCACTGAAGGTACTTCACTTACTGTTGGCAAAAAATATTGGGGCGGTGAAGAGGGAGATTCACTTTTCTACTGGTTCCGGAGTACTTCAGATGGAACTCCAACGGAAATTAGGGGTGCAACTACTGCTTCATACATGCTCTCAATTGATGACATTGGCTTCTTTATCTCAGTGTCGTGTGAGCCTGTCAGAAGTGACTGGGCGCGTGGTCCTACTGTTCTTTCCGAACAAATTGGACCTATAATACCTGGTCCCCCAACATGCCACTCTTTGGAGTTTCTTGGTTCAATGATTGAAGGACAACGCTTGAGCTTTATTGCTTCTTATACTGGAGGGGAGAAAGGAAATTGCTTCCATGAATGGTTTAGAGTGAAAAGTAATGGTGTCAAGGAGAAATTGAGTACTCAGGATTTTCTAGATTTGACTCTTGATGATGTGGGAAGATGCATTGAACTTGTTTACACCCCTATGCGCAAGGATGGGATGAGAGGAAATCCTATGAGCATAAAATCTGATGTGGTTGGCCCTGCGGATCCTGAAGGACTGGAGCTTATCATTCCTGTTTGCTGTGAGGATGAAGAATTGGTTCCAGAAAAAACATACTTTGGTGGAGAGGAGGGTGTTGGAGAATATATTTGGTACAGAACGAAGAATAAGCTTCATGGATCTGCATTGCTGGATATATCTAATTTGAATGAAGATGTTGGTATATGCAGTAAAACACTAACATATAAACCAGCACTTGAAGATGTTGGGGCTTATTTGGCCCTATATTGGGTGCCCACCCGTAAAGATGGTAAATGTGGGAAGGCACTAGTTGCAATTTGCAACTCTCCGGTTGCACCAGCTCTTCCTGTGGTTTCTAATGTTCGTGTGAAGAAGGTCTCTCTGTCTGTTTATTCTGGGGAAGGAGAGTATTTTGGTGGATATGAGGGATGGAGCCTTTTTAGCTGGTATAGAGAAACTAATGATGGAACTATCAGTCTTATTAATGGGGCCAACTCTCGAACTTATGAAGTCATTGATGCAGACTACAACTGCCGTTTATTGTTTGGATATACACCAGTTCGTTCAGATTCAGTGGTTGGTGAACTGAGGTTGTCTGAGCCAACTGACATTATTCTTCCAGAGCTTCCGAGACTAGAGATGCTTGCTCTCACTGGAAAAGCAATCGAAGGTGATGTTCTTACCGTTGTTGAAGTGATCCCAGAGAGTTTGACTCAACAGCTTGTTTGGCACAAGTACAAGCAAGATGTCAGATATCAATGGTTCTTTTCTTCAGCAGTGGGAGATAATAAGACCTTTGAACCATTACCTGCTCAGCGTTCTTGCTCCTACAAGATGCGATTAGAGGACGTCGGGCACTGTCTAAAATGTGAGTGCATTGTGACTGATGTGTTTGGAAGGTCAGCTGAGCCTGCATATGCTGAAACTGGACCAATACTACCAGGAATTCCTAGAATTGATAAGCTGGAGATCGAAGGGAGGGGTTTCCACACCAACTTATATGCTGTACGTGGCATTTATAGTGGAGGGAAGGAAGGCAAAAGTAGAATACAGTGGCTCAGATCAATGGTTGGGAGTCCTGATCTTATCTCTATACCCGGGGAGGTAGGGAGAATGTATGAATCCAATGTGGATGATGTAGGATATAGGCTTGTAGCCATTTACACTCCTGTAAGAGAGGATGGAGTGGAGGGGTCACCTGTTTCAGCATCGACAGAGCCGATTGCAGTCGGTAATAAAGAATCTCATCTTTTATTGAATTTTAGTTTTAATAAATTGCACATTATTTTGATCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000226 GO:0001578 GO:0001669 GO:0002177 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005856 GO:0006996 GO:0007010 GO:0007017 GO:0007276 GO:0007283 GO:0008150 GO:0009987 GO:0012505 GO:0015630 GO:0016043 GO:0019953 GO:0022414 GO:0022607 GO:0030030 GO:0030031 GO:0030141 GO:0031410 GO:0031982 GO:0032501 GO:0032504 GO:0035082 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043232 GO:0044085 GO:0044422 GO:0044424 GO:0044430 GO:0044444 GO:0044446 GO:0044464 GO:0044703 GO:0044782 GO:0048232 GO:0048609 GO:0051704 GO:0060271 GO:0070925 GO:0071840 GO:0097223 GO:0097708 GO:0099503 GO:0120031 GO:0120036

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

LRR_4

Protein Analysis

1598

Amino Acids

174.17

Weight (kDa)

5.96

Isoelectric Point (pI)

40.16

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LRR_4	PF12799	342 - 378	1.2e-06	Leucine Rich repeats (2 copies)
IG_AIR9	PF23197	497 - 579	3.3e-09	AIR9 A9 domain
IG_AIR9	PF23197	598 - 682	4.5e-20	AIR9 A9 domain
IG_AIR9	PF23197	694 - 780	9.7e-34	AIR9 A9 domain
IG_AIR9	PF23197	790 - 881	5e-14	AIR9 A9 domain
IG_AIR9	PF23197	892 - 978	1.2e-22	AIR9 A9 domain
IG_AIR9	PF23197	992 - 1071	2.8e-20	AIR9 A9 domain
IG_AIR9	PF23197	1086 - 1168	3.5e-24	AIR9 A9 domain
IG_AIR9	PF23197	1183 - 1271	2.2e-15	AIR9 A9 domain
IG_AIR9	PF23197	1296 - 1364	8.9e-16	AIR9 A9 domain
IG_AIR9	PF23197	1486 - 1571	5.7e-20	AIR9 A9 domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0006854)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G34680 AT2G34680
fragaria_vesca	FvH4_3g09950 FvH4_3g09950 FvH4_3g09950 FvH4_3g09950 FvH4_3g09950
malus_domestica	MD05G1274500.v1.1 MD10G1254200.v1.1
prunus_persica	Prupe.4G089200_v2.0.a1 Prupe.4G089200_v2.0.a1 Prupe.4G089200_v2.0.a1
pyrus_communis	pycom05g25590 pycom10g20990
rosa_chinensis	RchiOBHm_Chr5g0015701
rosa_laevigata	RLG00000032220
rosa_multiflora	Rmu_sc0000843.1_g000009
rosa_roxburghii	Rroxscaffold_1G00060820
rosa_rugosa	Rorug05G0029200 Rorug05G0029200 Rorug05G0029300
rosa_samantha	Rh5AG122500 Rh5BG121800 Rh5CG132000 Rh5DG120500
rosa_wichuraiana	Rw5G010580

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	1838, 2046
AatII	GACGTC	1 cut(s)	4353
Acc16I	TGCGCA	1 cut(s)	3467
Acc36I	ACCTGC	1 cut(s)	4318
AccB1I	GGYRCC	2 cut(s)	2024, 3772
AccB7I	CCANNNNNTGG	3 cut(s)	347, 1778, 3265
AccI	GTMKAC	5 cut(s)	514, 1153, 1662, 2003, 2243
AccII	CGCG	1 cut(s)	3199
AccIII	TCCGGA	2 cut(s)	2801, 3072
AciI	CCGC	6 cut(s)	376, 746, 1869, 2075, 3039, 3524
AclI	AACGTT	2 cut(s)	1433, 2091
AclWI	GGATC	8 cut(s)	681, 1568, 2734, 2812, 3521, 3534, 3667, 4192
AcyI	GRCGYC	1 cut(s)	4350
AflIII	ACRYGT	1 cut(s)	2944
AgeI	ACCGGT	1 cut(s)	1213
AhlI	ACTAGT	1 cut(s)	3808
AjiI	CACGTC	1 cut(s)	1059
AjnI	CCWGG	9 cut(s)	22, 118, 991, 1482, 1771, 2937, 2953, 3241, 4441
AjuI	GAANNNNNNNTTGG	4 cut(s)	176, 208, 1079, 1111
Alw21I	GWGCWC	1 cut(s)	2525
Alw26I	GTCTC	9 cut(s)	313, 384, 515, 637, 857, 2651, 2768, 3883, 4126
AlwI	GGATC	8 cut(s)	681, 1568, 2734, 2812, 3521, 3534, 3667, 4192
AlwNI	CAGNNNCTG	3 cut(s)	89, 368, 1778
Ama87I	CYCGRG	5 cut(s)	145, 205, 386, 1332, 4598
Aor13HI	TCCGGA	2 cut(s)	2801, 3072
ApaI	GGGCCC	1 cut(s)	2168
ApeKI	GCWGC	3 cut(s)	1166, 1274, 1421
AseI	ATTAAT	2 cut(s)	1583, 3984
AsiGI	ACCGGT	1 cut(s)	1213
Asp700I	GAANNNNTTC	3 cut(s)	1621, 2946, 3579
AspLEI	GCGC	2 cut(s)	3199, 3468
AsuC2I	CCSGG	5 cut(s)	463, 691, 1476, 4599, 4600
AvaI	CYCGRG	5 cut(s)	145, 205, 386, 1332, 4598
AvaII	GGWCC	8 cut(s)	149, 475, 826, 1295, 3203, 3230, 3245, 4430
AxyI	CCTNAGG	2 cut(s)	830, 2817
BaeGI	GKGCMC	3 cut(s)	2168, 3777, 4359
BaeI	ACNNNNGTAYC	4 cut(s)	2453, 2486, 2637, 2670
BamHI	GGATCC	1 cut(s)	3526
BanI	GGYRCC	2 cut(s)	2024, 3772
BanII	GRGCYC	1 cut(s)	2168
BbsI	GAAGAC	2 cut(s)	564, 693
Bbv12I	GWGCWC	1 cut(s)	2525
BbvCI	CCTCAGC	1 cut(s)	1077
BbvI	GCAGC	3 cut(s)	1153, 1286, 1433
BceAI	ACGGC	4 cut(s)	286, 1392, 2062, 4020
BcgI	CGANNNNNNTGC	2 cut(s)	2061, 2095
BciT130I	CCWGG	9 cut(s)	24, 120, 993, 1484, 1773, 2939, 2955, 3243, 4443
BclI	TGATCA	1 cut(s)	1504
BcnI	CCSGG	5 cut(s)	463, 691, 1476, 4599, 4600
BcoDI	GTCTC	9 cut(s)	313, 384, 515, 637, 857, 2651, 2768, 3883, 4126
BcuI	ACTAGT	1 cut(s)	3808
BfmI	CTRYAG	3 cut(s)	360, 666, 1154
BfuAI	ACCTGC	1 cut(s)	4318
BglII	AGATCT	2 cut(s)	1324, 2107
BisI	GCNGC	5 cut(s)	747, 1167, 1275, 1422, 1870
BlpI	GCTNAGC	2 cut(s)	4314, 4407
BlsI	GCNGC	5 cut(s)	748, 1168, 1276, 1423, 1871
BmcAI	AGTACT	2 cut(s)	3079, 3397
Bme18I	GGWCC	8 cut(s)	149, 475, 826, 1295, 3203, 3230, 3245, 4430
BmeT110I	CYCGRG	5 cut(s)	145, 205, 386, 1332, 4598
BmgBI	CACGTC	1 cut(s)	1059
BmrI	ACTGGG	5 cut(s)	29, 240, 2835, 2885, 3202
BmuI	ACTGGG	5 cut(s)	29, 240, 2835, 2885, 3202
BoxI	GACNNNNGTC	1 cut(s)	480
BpiI	GAAGAC	2 cut(s)	564, 693
BplI	GAGNNNNNCTC	4 cut(s)	1085, 1117, 4198, 4230
BpmI	CTGGAG	9 cut(s)	1674, 1860, 1959, 2457, 2589, 2715, 3345, 3560, 4487
Bpu10I	CCTNAGC	3 cut(s)	392, 1077, 1671
Bpu1102I	GCTNAGC	2 cut(s)	4314, 4407
BpuEI	CTTGAG	4 cut(s)	741, 839, 1241, 3322
BpuMI	CCSGG	5 cut(s)	463, 691, 1476, 4599, 4600
Bsa29I	ATCGAT	1 cut(s)	1980
BsaAI	YACGTR	1 cut(s)	4511
BsaHI	GRCGYC	1 cut(s)	4350
BsaI	GGTCTC	2 cut(s)	384, 3883
BsaJI	CCNNGG	8 cut(s)	119, 138, 144, 1788, 2538, 2953, 4598, 4599
BsaWI	WCCGGW	5 cut(s)	477, 1213, 2801, 3072, 3829
BsaXI	ACNNNNNCTCC	8 cut(s)	1806, 1836, 1946, 1976, 2097, 2127, 2650, 2680
Bse118I	RCCGGY	1 cut(s)	1213
Bse21I	CCTNAGG	2 cut(s)	830, 2817
Bse3DI	GCAATG	3 cut(s)	337, 1460, 3665
BseAI	TCCGGA	2 cut(s)	2801, 3072
BseBI	CCWGG	9 cut(s)	24, 120, 993, 1484, 1773, 2939, 2955, 3243, 4443
BseCI	ATCGAT	1 cut(s)	1980
BseDI	CCNNGG	8 cut(s)	119, 138, 144, 1788, 2538, 2953, 4598, 4599
BseMI	GCAATG	3 cut(s)	337, 1460, 3665
BseRI	GAGGAG	5 cut(s)	1253, 1918, 1967, 2148, 3623
BseSI	GKGCMC	3 cut(s)	2168, 3777, 4359
BseXI	GCAGC	3 cut(s)	1153, 1286, 1433
BseYI	CCCAGC	1 cut(s)	2626
BsgI	GTGCAG	1 cut(s)	1350
Bsh1236I	CGCG	1 cut(s)	3199
BshNI	GGYRCC	2 cut(s)	2024, 3772
BshTI	ACCGGT	1 cut(s)	1213
BshVI	ATCGAT	1 cut(s)	1980
BsiHKAI	GWGCWC	1 cut(s)	2525
BsiHKCI	CYCGRG	5 cut(s)	145, 205, 386, 1332, 4598
BsiSI	CCGG	9 cut(s)	462, 478, 690, 1214, 1475, 2802, 3073, 3830, 4599
BslFI	GGGAC	4 cut(s)	30, 135, 247, 3231
BsmAI	GTCTC	9 cut(s)	313, 384, 515, 637, 857, 2651, 2768, 3883, 4126
BsmFI	GGGAC	4 cut(s)	30, 135, 247, 3231
BsmI	GAATGC	1 cut(s)	1638
Bso31I	GGTCTC	2 cut(s)	384, 3883
BsoBI	CYCGRG	5 cut(s)	145, 205, 386, 1332, 4598
Bsp120I	GGGCCC	1 cut(s)	2164
Bsp1286I	GDGCHC	4 cut(s)	2168, 2525, 3777, 4359
Bsp13I	TCCGGA	2 cut(s)	2801, 3072
Bsp1407I	TGTACA	1 cut(s)	1549
Bsp1720I	GCTNAGC	2 cut(s)	4314, 4407
BspACI	CCGC	6 cut(s)	376, 746, 1869, 2075, 3039, 3524
BspDI	ATCGAT	1 cut(s)	1980
BspEI	TCCGGA	2 cut(s)	2801, 3072
BspFNI	CGCG	1 cut(s)	3199
BspHI	TCATGA	2 cut(s)	2749, 2782
BspMAI	CTGCAG	1 cut(s)	364
BspMI	ACCTGC	1 cut(s)	4318
BspPI	GGATC	8 cut(s)	681, 1568, 2734, 2812, 3521, 3534, 3667, 4192
BspQI	GCTCTTC	1 cut(s)	3849
BspT107I	GGYRCC	2 cut(s)	2024, 3772
BspTNI	GGTCTC	2 cut(s)	384, 3883
BsrDI	GCAATG	3 cut(s)	337, 1460, 3665
BsrFI	RCCGGY	1 cut(s)	1213
BsrGI	TGTACA	1 cut(s)	1549
BssAI	RCCGGY	1 cut(s)	1213
BssECI	CCNNGG	8 cut(s)	119, 138, 144, 1788, 2538, 2953, 4598, 4599
BssNAI	GTATAC	3 cut(s)	1663, 2004, 2244
BssNI	GRCGYC	1 cut(s)	4350
BssT1I	CCWWGG	3 cut(s)	138, 1788, 2538
Bst1107I	GTATAC	3 cut(s)	1663, 2004, 2244
Bst2UI	CCWGG	9 cut(s)	24, 120, 993, 1484, 1773, 2939, 2955, 3243, 4443
Bst6I	CTCTTC	3 cut(s)	2152, 3039, 3849
BstACI	GRCGYC	1 cut(s)	4350
BstAPI	GCANNNNNTGC	3 cut(s)	1856, 3119, 3812
BstAUI	TGTACA	1 cut(s)	1549
BstBAI	YACGTR	1 cut(s)	4511
BstC8I	GCNNGC	9 cut(s)	66, 549, 1065, 1171, 1290, 1419, 2355, 4146, 4413
BstEII	GGTNACC	3 cut(s)	465, 2621, 4695
BstENI	CCTNNNNNAGG	1 cut(s)	1487
BstFNI	CGCG	1 cut(s)	3199
BstHHI	GCGC	2 cut(s)	3199, 3468
BstMAI	GTCTC	9 cut(s)	313, 384, 515, 637, 857, 2651, 2768, 3883, 4126
BstMWI	GCNNNNNNNGC	9 cut(s)	298, 1856, 2958, 3119, 3758, 3812, 3942, 4228, 4412
BstNI	CCWGG	9 cut(s)	24, 120, 993, 1484, 1773, 2939, 2955, 3243, 4443
BstNSI	RCATGY	3 cut(s)	1421, 3133, 3258
BstPAI	GACNNNNGTC	1 cut(s)	480
BstPI	GGTNACC	3 cut(s)	465, 2621, 4695
BstSFI	CTRYAG	3 cut(s)	360, 666, 1154
BstSLI	GKGCMC	3 cut(s)	2168, 3777, 4359
BstUI	CGCG	1 cut(s)	3199
BstV1I	GCAGC	3 cut(s)	1153, 1286, 1433
BstV2I	GAAGAC	2 cut(s)	564, 693
BstX2I	RGATCY	6 cut(s)	686, 1324, 2107, 2804, 3526, 3659
BstYI	RGATCY	6 cut(s)	686, 1324, 2107, 2804, 3526, 3659
BstZ17I	GTATAC	3 cut(s)	1663, 2004, 2244
Bsu15I	ATCGAT	1 cut(s)	1980
Bsu36I	CCTNAGG	2 cut(s)	830, 2817
BsuTUI	ATCGAT	1 cut(s)	1980
BtgZI	GCGATG	1 cut(s)	2255
BtrI	CACGTC	1 cut(s)	1059
BtsI	GCAGTG	2 cut(s)	312, 4284
BveI	ACCTGC	1 cut(s)	4318
Cac8I	GCNNGC	9 cut(s)	66, 549, 1065, 1171, 1290, 1419, 2355, 4146, 4413
CaiI	CAGNNNCTG	3 cut(s)	89, 368, 1778
CciI	TCATGA	2 cut(s)	2749, 2782
CfoI	GCGC	2 cut(s)	3199, 3468
Cfr10I	RCCGGY	1 cut(s)	1213
Cfr9I	CCCGGG	1 cut(s)	4598
ClaI	ATCGAT	1 cut(s)	1980
CseI	GACGC	1 cut(s)	744
CsiI	ACCWGGT	2 cut(s)	1771, 3241
CspAI	ACCGGT	1 cut(s)	1213
CspCI	CAANNNNNGTGG	4 cut(s)	844, 879, 2410, 2445
DraI	TTTAAA	1 cut(s)	2473
Eam1104I	CTCTTC	3 cut(s)	2152, 3039, 3849
EarI	CTCTTC	3 cut(s)	2152, 3039, 3849
Eco130I	CCWWGG	3 cut(s)	138, 1788, 2538
Eco147I	AGGCCT	3 cut(s)	68, 143, 1496
Eco24I	GRGCYC	1 cut(s)	2168
Eco31I	GGTCTC	2 cut(s)	384, 3883
Eco32I	GATATC	2 cut(s)	1978, 4256
Eco47I	GGWCC	8 cut(s)	149, 475, 826, 1295, 3203, 3230, 3245, 4430
Eco81I	CCTNAGG	2 cut(s)	830, 2817
Eco88I	CYCGRG	5 cut(s)	145, 205, 386, 1332, 4598
Eco91I	GGTNACC	3 cut(s)	465, 2621, 4695
EcoNI	CCTNNNNNAGG	1 cut(s)	1487
EcoO109I	RGGNCCY	4 cut(s)	149, 989, 2164, 2165
EcoO65I	GGTNACC	3 cut(s)	465, 2621, 4695
EcoRI	GAATTC	3 cut(s)	250, 1782, 4446
EcoRII	CCWGG	9 cut(s)	22, 118, 991, 1482, 1771, 2937, 2953, 3241, 4441
EcoRV	GATATC	2 cut(s)	1978, 4256
EcoT14I	CCWWGG	3 cut(s)	138, 1788, 2538
EcoT22I	ATGCAT	2 cut(s)	1385, 3443
EcoT38I	GRGCYC	1 cut(s)	2168
ErhI	CCWWGG	3 cut(s)	138, 1788, 2538
FalI	AAGNNNNNCTT	6 cut(s)	1451, 1483, 1481, 1513, 1606, 1638
FaqI	GGGAC	4 cut(s)	30, 135, 247, 3231
FauNDI	CATATG	3 cut(s)	2632, 2731, 4417
FbaI	TGATCA	1 cut(s)	1504
FblI	GTMKAC	5 cut(s)	514, 1153, 1662, 2003, 2243
Fnu4HI	GCNGC	5 cut(s)	747, 1167, 1275, 1422, 1870
FriOI	GRGCYC	1 cut(s)	2168
Fsp4HI	GCNGC	5 cut(s)	747, 1167, 1275, 1422, 1870
FspI	TGCGCA	1 cut(s)	3467
GlaI	GCGC	2 cut(s)	3198, 3467
GluI	GCNGC	5 cut(s)	747, 1167, 1275, 1422, 1870
GsaI	CCCAGC	1 cut(s)	2630
GsuI	CTGGAG	9 cut(s)	1674, 1860, 1959, 2457, 2589, 2715, 3345, 3560, 4487
HapII	CCGG	9 cut(s)	462, 478, 690, 1214, 1475, 2802, 3073, 3830, 4599
HgaI	GACGC	1 cut(s)	744
HhaI	GCGC	2 cut(s)	3199, 3468
Hin1I	GRCGYC	1 cut(s)	4350
Hin6I	GCGC	2 cut(s)	3197, 3466
HinP1I	GCGC	2 cut(s)	3197, 3466
HincII	GTYRAC	2 cut(s)	1145, 1965
HindII	GTYRAC	2 cut(s)	1145, 1965
HindIII	AAGCTT	2 cut(s)	638, 3650
HpaII	CCGG	9 cut(s)	462, 478, 690, 1214, 1475, 2802, 3073, 3830, 4599
Hpy99I	CGWCG	4 cut(s)	1063, 2083, 2105, 4355
HpyCH4IV	ACGT	8 cut(s)	923, 1058, 1433, 2091, 2927, 2944, 4350, 4510
HpyF10VI	GCNNNNNNNGC	9 cut(s)	298, 1856, 2958, 3119, 3758, 3812, 3942, 4228, 4412
HpySE526I	ACGT	8 cut(s)	923, 1058, 1433, 2091, 2927, 2944, 4350, 4510
Hsp92I	GRCGYC	1 cut(s)	4350
HspAI	GCGC	2 cut(s)	3197, 3466
KflI	GGGWCCC	1 cut(s)	149
Kpn2I	TCCGGA	2 cut(s)	2801, 3072
Ksp22I	TGATCA	1 cut(s)	1504
LguI	GCTCTTC	1 cut(s)	3849
LmnI	GCTCC	7 cut(s)	1086, 1744, 1940, 2561, 3541, 3933, 4331
Lsp1109I	GCAGC	3 cut(s)	1153, 1286, 1433
MabI	ACCWGGT	2 cut(s)	1771, 3241
MaeII	ACGT	8 cut(s)	923, 1058, 1433, 2091, 2927, 2944, 4350, 4510
MfeI	CAATTG	3 cut(s)	1347, 2859, 3138
MflI	RGATCY	6 cut(s)	686, 1324, 2107, 2804, 3526, 3659
MhlI	GDGCHC	4 cut(s)	2168, 2525, 3777, 4359
MmeI	TCCRAC	5 cut(s)	804, 906, 2083, 3119, 3598
Mph1103I	ATGCAT	2 cut(s)	1385, 3443
MroI	TCCGGA	2 cut(s)	2801, 3072
MroXI	GAANNNNTTC	3 cut(s)	1621, 2946, 3579
MseI	TTAA	7 cut(s)	1304, 1583, 1589, 2372, 2472, 3984, 4770
MslI	CAYNNNNRTG	4 cut(s)	1378, 2597, 2881, 3461
MspA1I	CMGCKG	1 cut(s)	4406
MspI	CCGG	9 cut(s)	462, 478, 690, 1214, 1475, 2802, 3073, 3830, 4599
MunI	CAATTG	3 cut(s)	1347, 2859, 3138
Mva1269I	GAATGC	1 cut(s)	1638
MvaI	CCWGG	9 cut(s)	24, 120, 993, 1484, 1773, 2939, 2955, 3243, 4443
MvnI	CGCG	1 cut(s)	3199
MwoI	GCNNNNNNNGC	9 cut(s)	298, 1856, 2958, 3119, 3758, 3812, 3942, 4228, 4412
NciI	CCSGG	5 cut(s)	463, 691, 1476, 4599, 4600
NdeI	CATATG	3 cut(s)	2632, 2731, 4417
NmeAIII	GCCGAG	1 cut(s)	788
NsbI	TGCGCA	1 cut(s)	3467
NsiI	ATGCAT	2 cut(s)	1385, 3443
NspI	RCATGY	3 cut(s)	1421, 3133, 3258
PaeI	GCATGC	1 cut(s)	1421
PaeR7I	CTCGAG	1 cut(s)	205
PagI	TCATGA	2 cut(s)	2749, 2782
PceI	AGGCCT	3 cut(s)	68, 143, 1496
PciSI	GCTCTTC	1 cut(s)	3849
PcsI	WCGNNNNNNNCGW	3 cut(s)	212, 2097, 4718
PctI	GAATGC	1 cut(s)	1638
PdmI	GAANNNNTTC	3 cut(s)	1621, 2946, 3579
PflMI	CCANNNNNTGG	3 cut(s)	347, 1778, 3265
PinAI	ACCGGT	1 cut(s)	1213
PkrI	GCNGC	5 cut(s)	748, 1168, 1276, 1423, 1871
Ppu21I	YACGTR	1 cut(s)	4511
PpuMI	RGGWCCY	1 cut(s)	149
PshAI	GACNNNNGTC	1 cut(s)	480
PshBI	ATTAAT	2 cut(s)	1583, 3984
PsiI	TTATAA	2 cut(s)	1838, 2046
Psp1406I	AACGTT	2 cut(s)	1433, 2091
Psp5II	RGGWCCY	1 cut(s)	149
Psp6I	CCWGG	9 cut(s)	22, 118, 991, 1482, 1771, 2937, 2953, 3241, 4441
PspEI	GGTNACC	3 cut(s)	465, 2621, 4695
PspFI	CCCAGC	1 cut(s)	2626
PspGI	CCWGG	9 cut(s)	22, 118, 991, 1482, 1771, 2937, 2953, 3241, 4441
PspOMI	GGGCCC	1 cut(s)	2164
PspPPI	RGGWCCY	1 cut(s)	149
PspXI	VCTCGAGB	1 cut(s)	205
PstI	CTGCAG	1 cut(s)	364
PstNI	CAGNNNCTG	3 cut(s)	89, 368, 1778
PsuI	RGATCY	6 cut(s)	686, 1324, 2107, 2804, 3526, 3659
PvuII	CAGCTG	1 cut(s)	4406
RseI	CAYNNNNRTG	4 cut(s)	1378, 2597, 2881, 3461
SapI	GCTCTTC	1 cut(s)	3849
SaqAI	TTAA	7 cut(s)	1304, 1583, 1589, 2372, 2472, 3984, 4770
SatI	GCNGC	5 cut(s)	747, 1167, 1275, 1422, 1870
ScaI	AGTACT	2 cut(s)	3079, 3397
SduI	GDGCHC	4 cut(s)	2168, 2525, 3777, 4359
SexAI	ACCWGGT	2 cut(s)	1771, 3241
SfcI	CTRYAG	3 cut(s)	360, 666, 1154
Sfr274I	CTCGAG	1 cut(s)	205
SinI	GGWCC	8 cut(s)	149, 475, 826, 1295, 3203, 3230, 3245, 4430
SlaI	CTCGAG	1 cut(s)	205
SmaI	CCCGGG	1 cut(s)	4600
SmiMI	CAYNNNNRTG	4 cut(s)	1378, 2597, 2881, 3461
SmlI	CTYRAG	5 cut(s)	205, 756, 854, 1220, 3301
SmoI	CTYRAG	5 cut(s)	205, 756, 854, 1220, 3301
SpeI	ACTAGT	1 cut(s)	3808
SphI	GCATGC	1 cut(s)	1421
SseBI	AGGCCT	3 cut(s)	68, 143, 1496
SsiI	CCGC	6 cut(s)	376, 746, 1869, 2075, 3039, 3524
SspI	AATATT	4 cut(s)	1719, 1834, 2718, 3032
StuI	AGGCCT	3 cut(s)	68, 143, 1496
StyI	CCWWGG	3 cut(s)	138, 1788, 2538
TaiI	ACGT	8 cut(s)	926, 1061, 1436, 2094, 2930, 2947, 4353, 4513
TaqI	TCGA	7 cut(s)	206, 658, 1980, 4000, 4167, 4473, 4712
TaqII	GACCGA	1 cut(s)	2071
TatI	WGTACW	5 cut(s)	1549, 1709, 3077, 3395, 4236
TauI	GCSGC	2 cut(s)	749, 1872
Tru1I	TTAA	7 cut(s)	1304, 1583, 1589, 2372, 2472, 3984, 4770
Tru9I	TTAA	7 cut(s)	1304, 1583, 1589, 2372, 2472, 3984, 4770
TseI	GCWGC	3 cut(s)	1166, 1274, 1421
TspGWI	ACGGA	2 cut(s)	547, 3113
TspMI	CCCGGG	1 cut(s)	4598
Van91I	CCANNNNNTGG	3 cut(s)	347, 1778, 3265
VpaK11BI	GGWCC	8 cut(s)	149, 475, 826, 1295, 3203, 3230, 3245, 4430
VspI	ATTAAT	2 cut(s)	1583, 3984
XagI	CCTNNNNNAGG	1 cut(s)	1487
XbaI	TCTAGA	1 cut(s)	3409
XceI	RCATGY	3 cut(s)	1421, 3133, 3258
XhoI	CTCGAG	1 cut(s)	205
XmaI	CCCGGG	1 cut(s)	4598
XmiI	GTMKAC	5 cut(s)	514, 1153, 1662, 2003, 2243
XmnI	GAANNNNTTC	3 cut(s)	1621, 2946, 3579
ZraI	GACGTC	1 cut(s)	4351
ZrmI	AGTACT	2 cut(s)	3079, 3397
Zsp2I	ATGCAT	2 cut(s)	1385, 3443

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.