Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0006854

Physical Location & Seq

Genomic Coordinates Forward (+)

34657411 .. 34670126

Gene Structure 12716 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1254200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 5148 bp

ATGGAGGACAATTTAGTGCCTGCGGGCGAAGACACAGTTGAAAAGGTCCCAATTCCAAAGAAGCAGTCCTCTGTTTGTTCATCAGAAACTGCAAAAAGTGTTTCCAAAAAGGTGAAACCTGGTGGTGCAGTGACATCGAAGATCTCGGTTCCAACTAGTTCCATTAGACCGAAAGTAGACCCGAAAAGTGGTTTGGATCCTAATTTAAGTGTCACCAAGCCAAGTGCAACTGGCTCTACCCGGAGTTTGAATTCAGTCCCTGTAAGGCGCAACAGCACTGGAGGTTTGCCTCAGAAGCCATCAGTCTCCGCCGCAAGAATGCAAAACAATACTACTACGGCTGCAGTTAAAAAGACTCCGGACGCTGTAAGGCGGTCTCTCCCAGAGCTTAGAAGAAGTTCAGTGCCTTCAGCTGCTACTGCTAAATCTTTGACTAGAACCAGCGTTTCAGAGGTTAGAAAGTCGGTTTCAGGATCGCCATTGGACCGGAGCCTGAATAAATCAACTGGATCTAATGTGAGTGTAACTAAACAAGAGACTATCAGGAAGCCTGCTGTGAGGCCAGTATTGTCCACTTCTTCTTCATCTTCAAGAAGGCCGACTTCCTCATTGGACAGTAGTGCTAGCAGTGGTGTCAGGAAGTCGGTCTCAAAGCTTTCTTCCCCATCAGCTCGATCACCTGCAGTTACTAGTGGGCTGAGAACTTTGTCCTCTTCTCTTGATAGAAATTCCAGTTTATCTGGACGTAGGAAAACAGCAACTCCTGAACACCGGGATTCTCGGTTCATTGTACTTCCACAAGTGGAAATCAAAGCCGGTGATGATCTGAGGTTAGATCTTAGGGGTCACAGAGTTCGTAGCCTCAAGGCTAGCGGGTTGAACTTGTCGCCAAATTTAGAGTTTGTTTATCTTAGAGACAATCTCTTGTCTACGTTGGAGGGAGTGGAAATATTGACACGAGTAAAGGTTCTGGATTTGAGCTTCAATGATTTCAAAGGGCCTGGATTTGAACCTCTTGAAAATTGCAAAGTCTTACAGCAACTTTATCTTGCTGGAAACCAAATCACATCACTTGCAAGCCTCCCTCAGCTCCCTAATTTGGAGTTTCTCTCTGTCGCTCAAAATAAGTTGAAGTCTCTTACAATGGCAAGCCAACCTAGATTACAGGTATTAGCTGCCAGCAAAAACAAAATTTCAACTCTAAAGGGTTTCCCCTACTTACCAGTCCTTGAGCATTTACGAGTGGAGGAGAATCCCATTCTTAAAATGCCTCACTTAGAAGCAGCTTCCATTTTGCTTGTTGGCCCTACCTTAAAAAAGTTCAATGATAGAGATCTTTCCCGAGAAGAGCTTACAATTGCAAAGCGCTATCCTGCACACACCTCTTTGTGCATTAGAGATGGTTGGGAGTTTTGCCGTCCAGACCATGCAGCAGACTCAACTTTTCGTTTCCTTGTTGAGCAATGGAAGGATCATTTGCCTCCTGGCTTCCTGGTCAAGGAGGCCTCTGTTGAGAAACCATTTGAAGAAGATACCTGTCGCTGCCAGTTCACTTTTGTACAAGAGAACACTTTGGCAACTGATCCACAATTAATCTTAAAATACCAATGGTTTGTAGGAGAAAGAACTCCTTCAAATTTCACCATTATTCCTGATGCCACTGGAGAGGTCTACTGGCCTAAGCATGACGATATCGGTAGAATTTTAAAAGTGGAGTGTACTCCCGTATTAGGAGAAATGGAGTATCCTCCTATTTTTGCTATATCTTCTCCAGTTAAACGAGGAAGTGGAATCCCAAAGGTTGTGAATCTTGATGTTCGTGGAGATCTGATGGAGGGAAATATTATGAGAGGCCATGCAGAGGTTGCGTGGTGTGGTGGTACTCCAGGGAAAGGTGTTTCTAGTTGGTTGAGGAGAAAATGGAATAGCAGTCCTGTGGTTATTGCTGGAGCTGAAGATGAGGAGTACAAATTGACTATAGATGATATTGATTCGAGTTTGGTTTTTATGTACACACCAGTGACAGAAGAAGGTGCCAAAGGAGAACCTCATTATAAATACACCGATTTTGTAAAATCAGCCCCTCCATCAGTCAATAATGTACACATTGTTGGAGATGTTGTTGAAGGCAGTACCATCAGAGGAGTTGGTGATTACTTTGGAGGAAGAGAGGGTCCCAGCAAGTTTGAATGGTTATGTGAGCATAAAGACACTGGAGACCTCGTTTTGGTATCAACTGGTACATCTGAGTATACTTTGACTAAGGAAGATGTTGGCCATCACTTGGCTTTTGTGTATATACCTATCAATTTTGAAGGTCAAGAAGGGGAATCTGTTTCGATAAGGTCCCATGTTGTGAAGCCAGCACCTCCAAAGGTAATAGATTTGAAGATAGTTGGTGATCTAAGGGAGAACAGTAAGGTTACTGCAGTCGGTACTGTCACTGGAGGAACTGAAGGGTCTAGCAGAGTACAGTGGTTTAAAACAAGTTCGTCTACTTTGGATGGTGAGAAAGGTCTTGAATCTTTGAGCACCTCTAAGATTGCAAAGGCGTTTCGCGTACCCTTAGGAGCTGTTGGCTATTACATCGTTGCAAAATTTACTCCTATGACTCTAGACGGTGAATCTGGTGAACCAGCGTATGCTATATCAGATAGAGCAGTTGAGACTCTGCCTCCAAGCCTAAATTTCTTATCAATTACCGGTGATTACACCGAAGGTGGAATGTTGACGGCATCATATGGTTACATTGGTGGTCATGAAGGAAAAAGTATCTATAATTGGTATCTTCATGAGGTTGAGGCTGACTGCGGATCTCTAATACCTGAGGTCACAGGACTTCTTCAGTATCGTATTGCAAAAGATGCAATTGGTAAATTCATTTCATTCCAATGCACACCAGTGCGTGATGATGGGATTGTGGGTGAGCCAAGAACATGCATGGGCCAGGAATGTGTTCGCCCTGGGAACCCTAGATTGCTTTCTCTACAAATCGTTGGGAATGCCACTGAAGGAACTACACTTAGTGTGGAAAAAAAATATTGGGGCGGTGAAGAGGGAGATTCAGTTTTCTACTGGTTCCGGACTACCTCAGATGGAGCACAAACTGAAATTAGGGGTGCAAAAGCTGCTTCATACACTCTCTCAATTGATGATATTGGCTTCTTTGTCTCAGTGTCATGTGAGCCTGTCAGAAGTGACTGGGCTCGTGGTCCCACTGTTCTATCCGAACAAATTGGACCTGTTATACCTGGCCCCCCAACTTGTCGATCTCTGGAGTTTCTTGGTTCCATGATAGAAGGGCAACGTCTCAGCTTTACTGCTTCTTATAGTGGAGGGGAGAAAGGAAATTGCTCCCACGAATGGTTTAGAGTGAAGCGTAATGGTGTCAAGGAGAAGTTGAGTACTCAAGGACACGATTTTGTGGATTTGACCCTTGATGATGTGGGAACATGTGTTGAACTTGTTTACACTCCTATGCGCAAAGATGGGATGAAAGGAAATTCTAAGAGTATACAATCTGATGTGATTGCTCCTGCGGATCCTGAAGGGCTGGAGCTTGTCATTCCCGACTGCTGTGAGGCTGAAAATTTACACCCAAAAAAAAGATATTTTGGTGGAGAAGAAGGTGTTGGAGAATACATTTGGTACAGAACAAAGAATAAGCTGCATGGATCTGCATTGCAGGATATATCTAATGTGTGTGAAGATTTGGTTATATGTGGTAAAGCATTAACATACACACCAGTACTTGAAGATGTGGAGGCATATTTAGCCTTGCATTGGCTCCCCACTCGTTCTGATGGCAAATGTGGACAGCCACTAGTTGCGATTTGCAACTTTCCAGTTGCACCAGCTCTTCCTGTGGTTTCTAATGTTCGTGTGAAGGAGTTGTCACGAAGTGTTTATTCTGGAGAAGGAGAGTATTTTGGTGGATATGAAGGATCGAGCCTGTTTAGCTGGTATAGAGAAAATAATGAAGGAACTATTGCTCTTATTAATGGAGCCAACTCTAACACTTATGAAGTCACCGATGCAGACTACAACTGCCGTCTATTGTTTGGATACACACCGGTTCGTTCAGATTCGGTGGTTGGAGAACTGAAGTTGTCTGAGCCAACTGATATTATTCTTCCAGAGCTTCCAAGACTAGAGATGCTCGCTCTCACTGGAAAGGCAATCGAAGGTGATGTTCTTACAGTTGTTCAAGTGATCCCAGAGAGTGAGACTCAACAGATTGTTTGGAGCAAGTACAAGAAAGATGTCAGATATCAATGGTATTTTTCTTCAAAGGAGGAAGATATGAAAACCTTTGAAATATTACCTGCGCAGCAATCTTGCTCCTACAAGATGCGTCTAGAAGATGTTGGGCGCTGTCTAAAATGTGAGTGCATTGTGACTGATGTCTTTGGAAGATCAACTGAGCCTGTATATGCTGAAACTGGACCTATATTACCAGGGATCCCTAGGATAGATAAGCTGGAGATTGAAGGGAGGGGTTTCCACACCAACTTATACGCTGTACGTGGAATTTATAGCGGAGGGAAGGAAGGCAAAAGTAGAATCCAGTGGCTCAGATCAATGGTTGGGAGTCCTGATCTTATCTCTATACCAGGGGAGGTAGGGAGAATGTATGAGTCTAATGTGGACGACGTAGGGTACAGGCTTGTTGTGGTTTACACTCCTGTAAGAGAGGATGGAGTGGAGGGGCAACCTGTTTCTGCATCAACAGACCCGATTGCAGTTGAACCTGATGTTCTTAAAGAAGTGAGGCAGAAGCTTGATCTTGGAACTGTGAAATTTGAGACATTGTGTGACAAGGACCAATCTACGAAAAAGGCTCCTGCAGTGGGAAGTCTTGAGAGAAGAATCCTCGAAGTCAATAGAAAAAGAGTCAAGGTGGTAAAGCCTGGTTCCAAGACTTCCTTCCCAACGACTGAAATTCGTGGAAGTTATGCGCCTCCCTTTCACGTGGAGCTGTTCCGAAACGACCAACATCGTCTGAAAATCGTGGTGGACGGCGAGAATGAGGTGGACTTGATGGTGCAGTCGAGGCACCTGCGGGATGTGACAGTGCTTGTGATCCGAGGGCTCGCTCAGAGATTCAACAGTACATCCCTCAACACCCTCCTCAAAATCGAGACATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000226 GO:0001578 GO:0001669 GO:0002177 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005856 GO:0006996 GO:0007010 GO:0007017 GO:0007276 GO:0007283 GO:0008150 GO:0009987 GO:0012505 GO:0015630 GO:0016043 GO:0019953 GO:0022414 GO:0022607 GO:0030030 GO:0030031 GO:0030141 GO:0031410 GO:0031982 GO:0032501 GO:0032504 GO:0035082 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043232 GO:0044085 GO:0044422 GO:0044424 GO:0044430 GO:0044444 GO:0044446 GO:0044464 GO:0044703 GO:0044782 GO:0048232 GO:0048609 GO:0051704 GO:0060271 GO:0070925 GO:0071840 GO:0097223 GO:0097708 GO:0099503 GO:0120031 GO:0120036

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

LRR_4

Protein Analysis

1716

Amino Acids

188.24

Weight (kDa)

6.5

Isoelectric Point (pI)

38.81

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LRR_4	PF12799	345 - 381	1.1e-06	Leucine Rich repeats (2 copies)
IG_AIR9	PF23197	503 - 588	1.5e-09	AIR9 A9 domain
IG_AIR9	PF23197	601 - 685	7e-21	AIR9 A9 domain
IG_AIR9	PF23197	697 - 782	7.3e-34	AIR9 A9 domain
IG_AIR9	PF23197	793 - 884	5.9e-16	AIR9 A9 domain
IG_AIR9	PF23197	895 - 981	6e-22	AIR9 A9 domain
IG_AIR9	PF23197	995 - 1074	1.6e-21	AIR9 A9 domain
IG_AIR9	PF23197	1089 - 1173	1.6e-23	AIR9 A9 domain
IG_AIR9	PF23197	1188 - 1277	5.3e-13	AIR9 A9 domain
IG_AIR9	PF23197	1301 - 1369	4.5e-18	AIR9 A9 domain
IG_AIR9	PF23197	1491 - 1576	7.1e-21	AIR9 A9 domain
PH_AIR9	PF23218	1606 - 1702	1.6e-52	AIR9 PH-like domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0006854)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G34680 AT2G34680
fragaria_vesca	FvH4_3g09950 FvH4_3g09950 FvH4_3g09950 FvH4_3g09950 FvH4_3g09950
malus_domestica	MD05G1274500.v1.1 MD10G1254200.v1.1
prunus_persica	Prupe.4G089200_v2.0.a1 Prupe.4G089200_v2.0.a1 Prupe.4G089200_v2.0.a1
pyrus_communis	pycom05g25590 pycom10g20990
rosa_chinensis	RchiOBHm_Chr5g0015701
rosa_laevigata	RLG00000032220
rosa_multiflora	Rmu_sc0000843.1_g000009
rosa_roxburghii	Rroxscaffold_1G00060820
rosa_rugosa	Rorug05G0029200 Rorug05G0029200 Rorug05G0029300
rosa_samantha	Rh5AG122500 Rh5BG121800 Rh5CG132000 Rh5DG120500
rosa_wichuraiana	Rw5G010580

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2055
AarI	CACCTGC	2 cut(s)	688, 5067
Acc16I	TGCGCA	2 cut(s)	3482, 4329
Acc36I	ACCTGC	3 cut(s)	688, 4333, 5067
AccB1I	GGYRCC	2 cut(s)	2033, 5055
AccB7I	CCANNNNNTGG	1 cut(s)	2284
AccI	GTMKAC	6 cut(s)	177, 929, 1671, 2252, 2495, 3514
AccII	CGCG	1 cut(s)	2559
AccIII	TCCGGA	2 cut(s)	358, 3081
AcoI	YGGCCR	1 cut(s)	2275
AcuI	CTGAAG	7 cut(s)	393, 1974, 2475, 2828, 3030, 3567, 4124
AcvI	CACGTG	1 cut(s)	4972
AdeI	CACNNNGTG	2 cut(s)	3026, 3902
AfeI	AGCGCT	1 cut(s)	1367
AflIII	ACRYGT	1 cut(s)	3452
AgeI	ACCGGT	2 cut(s)	2702, 4072
AhlI	ACTAGT	3 cut(s)	155, 689, 3823
AjuI	GAANNNNNNNTTGG	6 cut(s)	176, 208, 3615, 3647, 4910, 4942
AleI	CACNNNNGTG	2 cut(s)	2018, 2900
AloI	GAACNNNNNNTCC	2 cut(s)	767, 799
Alw21I	GWGCWC	2 cut(s)	2534, 3103
AlwNI	CAGNNNCTG	2 cut(s)	89, 260
Ama87I	CYCGRG	1 cut(s)	1341
Aor13HI	TCCGGA	2 cut(s)	358, 3081
Aor51HI	AGCGCT	1 cut(s)	1367
ApeKI	GCWGC	9 cut(s)	341, 413, 1175, 1283, 1430, 1542, 3128, 3667, 4330
ArsI	GACNNNNNNTTYG	2 cut(s)	50, 82
AseI	ATTAAT	2 cut(s)	1592, 3999
AsiGI	ACCGGT	2 cut(s)	2702, 4072
Asp700I	GAANNNNTTC	3 cut(s)	397, 1630, 2955
AspA2I	CCTAGG	1 cut(s)	4466
AspLEI	GCGC	6 cut(s)	270, 1368, 3483, 4330, 4374, 4960
AsuC2I	CCSGG	2 cut(s)	241, 773
AsuNHI	GCTAGC	2 cut(s)	623, 869
AvaI	CYCGRG	1 cut(s)	1341
AvaII	GGWCC	8 cut(s)	46, 484, 2174, 2346, 3212, 3239, 4445, 4822
AvrII	CCTAGG	1 cut(s)	4466
AxyI	CCTNAGG	2 cut(s)	2566, 2826
BalI	TGGCCA	1 cut(s)	2277
BamHI	GGATCC	3 cut(s)	196, 3541, 4461
BanI	GGYRCC	2 cut(s)	2033, 5055
BanII	GRGCYC	2 cut(s)	3208, 5094
BauI	CACGAG	2 cut(s)	957, 3207
BbrPI	CACGTG	1 cut(s)	4972
BbsI	GAAGAC	1 cut(s)	36
Bbv12I	GWGCWC	2 cut(s)	2534, 3103
BbvCI	CCTCAGC	1 cut(s)	1086
BbvI	GCAGC	9 cut(s)	328, 400, 1162, 1295, 1442, 1529, 3115, 3654, 4342
BceAI	ACGGC	5 cut(s)	354, 1401, 2748, 4035, 5035
BcgI	CGANNNNNNTGC	4 cut(s)	117, 151, 5041, 5075
BciVI	GTATCC	2 cut(s)	1755, 4058
BcnI	CCSGG	2 cut(s)	241, 773
BcuI	ACTAGT	3 cut(s)	155, 689, 3823
BfmI	CTRYAG	5 cut(s)	342, 681, 1977, 2427, 4845
BfoI	RGCGCY	2 cut(s)	1369, 4375
BfuAI	ACCTGC	3 cut(s)	688, 4333, 5067
BfuI	GTATCC	2 cut(s)	1755, 4058
BglII	AGATCT	4 cut(s)	141, 835, 1333, 1825
BlnI	CCTAGG	1 cut(s)	4466
BmcAI	AGTACT	2 cut(s)	3406, 3750
Bme18I	GGWCC	8 cut(s)	46, 484, 2174, 2346, 3212, 3239, 4445, 4822
BmeT110I	CYCGRG	1 cut(s)	1341
BmrI	ACTGGG	1 cut(s)	3211
BmsI	GCATC	7 cut(s)	1645, 2744, 2854, 4024, 4146, 4341, 4733
BmtI	GCTAGC	2 cut(s)	627, 873
BmuI	ACTGGG	1 cut(s)	3211
BoxI	GACNNNNGTC	1 cut(s)	4403
BpiI	GAAGAC	1 cut(s)	36
BplI	GAGNNNNNCTC	6 cut(s)	906, 938, 1094, 1126, 4213, 4245
Bpu10I	CCTNAGC	2 cut(s)	1086, 1680
BpuEI	CTTGAG	4 cut(s)	848, 1250, 3393, 4880
BpuMI	CCSGG	2 cut(s)	241, 773
BsaAI	YACGTR	2 cut(s)	4526, 4972
BsaBI	GATNNNNATC	1 cut(s)	1332
BsaI	GGTCTC	3 cut(s)	381, 652, 2211
BsaJI	CCNNGG	7 cut(s)	1886, 2962, 2963, 4457, 4466, 4613, 5086
BsaWI	WCCGGW	5 cut(s)	358, 486, 2702, 3081, 4072
Bse118I	RCCGGY	3 cut(s)	815, 2702, 4072
Bse21I	CCTNAGG	2 cut(s)	2566, 2826
Bse3DI	GCAATG	2 cut(s)	1469, 3680
Bse8I	GATNNNNATC	1 cut(s)	1332
BseAI	TCCGGA	2 cut(s)	358, 3081
BseDI	CCNNGG	7 cut(s)	1886, 2962, 2963, 4457, 4466, 4613, 5086
BseGI	GGATG	5 cut(s)	2509, 3498, 4702, 5071, 5114
BseJI	GATNNNNATC	1 cut(s)	1332
BseMI	GCAATG	2 cut(s)	1469, 3680
BseRI	GAGGAG	5 cut(s)	1262, 1927, 1976, 2157, 5120
BseXI	GCAGC	9 cut(s)	328, 400, 1162, 1295, 1442, 1529, 3115, 3654, 4342
BseYI	CCCAGC	1 cut(s)	2177
BsgI	GTGCAG	3 cut(s)	147, 1359, 5066
Bsh1236I	CGCG	1 cut(s)	2559
BshNI	GGYRCC	2 cut(s)	2033, 5055
BshTI	ACCGGT	2 cut(s)	2702, 4072
BsiHKAI	GWGCWC	2 cut(s)	2534, 3103
BsiHKCI	CYCGRG	1 cut(s)	1341
BsiSI	CCGG	8 cut(s)	241, 359, 487, 772, 816, 2703, 3082, 4073
BslFI	GGGAC	5 cut(s)	32, 242, 2160, 2332, 3198
BsmBI	CGTCTC	1 cut(s)	3314
BsmFI	GGGAC	5 cut(s)	32, 242, 2160, 2332, 3198
BsmI	GAATGC	2 cut(s)	324, 3007
Bso31I	GGTCTC	3 cut(s)	381, 652, 2211
BsoBI	CYCGRG	1 cut(s)	1341
Bsp1286I	GDGCHC	4 cut(s)	2534, 3103, 3208, 5094
Bsp13I	TCCGGA	2 cut(s)	358, 3081
Bsp1407I	TGTACA	3 cut(s)	1558, 2010, 2101
BspEI	TCCGGA	2 cut(s)	358, 3081
BspFNI	CGCG	1 cut(s)	2559
BspHI	TCATGA	2 cut(s)	2758, 2791
BspMAI	CTGCAG	4 cut(s)	346, 685, 2431, 4849
BspMI	ACCTGC	3 cut(s)	688, 4333, 5067
BspOI	GCTAGC	2 cut(s)	627, 873
BspQI	GCTCTTC	2 cut(s)	1341, 3864
BspT107I	GGYRCC	2 cut(s)	2033, 5055
BspTNI	GGTCTC	3 cut(s)	381, 652, 2211
BsrDI	GCAATG	2 cut(s)	1469, 3680
BsrFI	RCCGGY	3 cut(s)	815, 2702, 4072
BsrGI	TGTACA	3 cut(s)	1558, 2010, 2101
BssAI	RCCGGY	3 cut(s)	815, 2702, 4072
BssECI	CCNNGG	7 cut(s)	1886, 2962, 2963, 4457, 4466, 4613, 5086
BssNAI	GTATAC	2 cut(s)	2253, 3515
BssSI	CACGAG	2 cut(s)	957, 3207
BssT1I	CCWWGG	1 cut(s)	4466
Bst1107I	GTATAC	2 cut(s)	2253, 3515
Bst2BI	CACGAG	2 cut(s)	957, 3207
Bst6I	CTCTTC	5 cut(s)	718, 1341, 2161, 3048, 3864
BstAPI	GCANNNNNTGC	3 cut(s)	1865, 2864, 3128
BstAUI	TGTACA	3 cut(s)	1558, 2010, 2101
BstBAI	YACGTR	2 cut(s)	4526, 4972
BstENI	CCTNNNNNAGG	1 cut(s)	1496
BstF5I	GGATG	5 cut(s)	2509, 3498, 4702, 5071, 5114
BstFNI	CGCG	1 cut(s)	2559
BstH2I	RGCGCY	2 cut(s)	1369, 4375
BstHHI	GCGC	6 cut(s)	270, 1368, 3483, 4330, 4374, 4960
BstMWI	GCNNNNNNNGC	8 cut(s)	295, 419, 1865, 2864, 3128, 3773, 3957, 5053
BstNSI	RCATGY	2 cut(s)	2940, 3456
BstPAI	GACNNNNGTC	1 cut(s)	4403
BstSFI	CTRYAG	5 cut(s)	342, 681, 1977, 2427, 4845
BstUI	CGCG	1 cut(s)	2559
BstV1I	GCAGC	9 cut(s)	328, 400, 1162, 1295, 1442, 1529, 3115, 3654, 4342
BstV2I	GAAGAC	1 cut(s)	36
BstZ17I	GTATAC	2 cut(s)	2253, 3515
Bsu36I	CCTNAGG	2 cut(s)	2566, 2826
BsuI	GTATCC	2 cut(s)	1755, 4058
BtsCI	GGATG	5 cut(s)	2509, 3498, 4702, 5071, 5114
BtsI	GCAGTG	3 cut(s)	135, 634, 4854
BveI	ACCTGC	3 cut(s)	688, 4333, 5067
CaiI	CAGNNNCTG	2 cut(s)	89, 260
CciI	TCATGA	2 cut(s)	2758, 2791
CfoI	GCGC	6 cut(s)	270, 1368, 3483, 4330, 4374, 4960
Cfr10I	RCCGGY	3 cut(s)	815, 2702, 4072
CseI	GACGC	2 cut(s)	371, 4343
CsiI	ACCWGGT	1 cut(s)	118
CspAI	ACCGGT	2 cut(s)	2702, 4072
DraI	TTTAAA	2 cut(s)	1707, 2482
DraIII	CACNNNGTG	2 cut(s)	3026, 3902
EaeI	YGGCCR	1 cut(s)	2275
Eam1104I	CTCTTC	5 cut(s)	718, 1341, 2161, 3048, 3864
EarI	CTCTTC	5 cut(s)	718, 1341, 2161, 3048, 3864
EciI	GGCGGA	1 cut(s)	298
Eco130I	CCWWGG	1 cut(s)	4466
Eco147I	AGGCCT	1 cut(s)	1505
Eco24I	GRGCYC	2 cut(s)	3208, 5094
Eco31I	GGTCTC	3 cut(s)	381, 652, 2211
Eco32I	GATATC	2 cut(s)	1693, 4271
Eco47I	GGWCC	8 cut(s)	46, 484, 2174, 2346, 3212, 3239, 4445, 4822
Eco47III	AGCGCT	1 cut(s)	1367
Eco57I	CTGAAG	7 cut(s)	393, 1974, 2475, 2828, 3030, 3567, 4124
Eco72I	CACGTG	1 cut(s)	4972
Eco81I	CCTNAGG	2 cut(s)	2566, 2826
Eco88I	CYCGRG	1 cut(s)	1341
EcoNI	CCTNNNNNAGG	1 cut(s)	1496
EcoO109I	RGGNCCY	4 cut(s)	46, 998, 2174, 2346
EcoRI	GAATTC	1 cut(s)	250
EcoRV	GATATC	2 cut(s)	1693, 4271
EcoT14I	CCWWGG	1 cut(s)	4466
EcoT22I	ATGCAT	1 cut(s)	2942
EcoT38I	GRGCYC	2 cut(s)	3208, 5094
ErhI	CCWWGG	1 cut(s)	4466
Esp3I	CGTCTC	1 cut(s)	3314
FalI	AAGNNNNNCTT	6 cut(s)	586, 618, 1615, 1647, 4910, 4942
FaqI	GGGAC	5 cut(s)	32, 242, 2160, 2332, 3198
FauI	CCCGC	3 cut(s)	16, 866, 5055
FauNDI	CATATG	1 cut(s)	2740
FblI	GTMKAC	6 cut(s)	177, 929, 1671, 2252, 2495, 3514
FokI	GGATG	5 cut(s)	2516, 3505, 4709, 5078, 5101
FriOI	GRGCYC	2 cut(s)	3208, 5094
FspI	TGCGCA	2 cut(s)	3482, 4329
GlaI	GCGC	6 cut(s)	269, 1367, 3482, 4329, 4373, 4959
GsaI	CCCAGC	1 cut(s)	2181
HaeII	RGCGCY	2 cut(s)	1369, 4375
HapII	CCGG	8 cut(s)	241, 359, 487, 772, 816, 2703, 3082, 4073
HgaI	GACGC	2 cut(s)	371, 4343
HhaI	GCGC	6 cut(s)	270, 1368, 3483, 4330, 4374, 4960
Hin6I	GCGC	6 cut(s)	268, 1366, 3481, 4328, 4372, 4958
HinP1I	GCGC	6 cut(s)	268, 1366, 3481, 4328, 4372, 4958
HincII	GTYRAC	1 cut(s)	2730
HindII	GTYRAC	1 cut(s)	2730
HindIII	AAGCTT	2 cut(s)	653, 4778
HpaII	CCGG	8 cut(s)	241, 359, 487, 772, 816, 2703, 3082, 4073
Hpy99I	CGWCG	1 cut(s)	4655
HpyCH4IV	ACGT	6 cut(s)	745, 932, 3307, 4525, 4653, 4971
HpyF10VI	GCNNNNNNNGC	8 cut(s)	295, 419, 1865, 2864, 3128, 3773, 3957, 5053
HpySE526I	ACGT	6 cut(s)	745, 932, 3307, 4525, 4653, 4971
HspAI	GCGC	6 cut(s)	268, 1366, 3481, 4328, 4372, 4958
KflI	GGGWCCC	1 cut(s)	2174
Kpn2I	TCCGGA	2 cut(s)	358, 3081
LguI	GCTCTTC	2 cut(s)	1341, 3864
Lsp1109I	GCAGC	9 cut(s)	328, 400, 1162, 1295, 1442, 1529, 3115, 3654, 4342
LweI	GCATC	7 cut(s)	1645, 2744, 2854, 4024, 4146, 4341, 4733
MabI	ACCWGGT	1 cut(s)	118
MaeII	ACGT	6 cut(s)	745, 932, 3307, 4525, 4653, 4971
MfeI	CAATTG	3 cut(s)	1356, 2868, 3147
MhlI	GDGCHC	4 cut(s)	2534, 3103, 3208, 5094
MlsI	TGGCCA	1 cut(s)	2277
MluNI	TGGCCA	1 cut(s)	2277
MlyI	GAGTC	8 cut(s)	349, 1430, 2605, 2662, 4222, 4600, 4646, 4902
MmeI	TCCRAC	5 cut(s)	176, 915, 2092, 3613, 4075
Mox20I	TGGCCA	1 cut(s)	2277
Mph1103I	ATGCAT	1 cut(s)	2942
MroI	TCCGGA	2 cut(s)	358, 3081
MroXI	GAANNNNTTC	3 cut(s)	397, 1630, 2955
MscI	TGGCCA	1 cut(s)	2277
MslI	CAYNNNNRTG	4 cut(s)	2018, 2890, 2900, 3476
Msp20I	TGGCCA	1 cut(s)	2277
MspA1I	CMGCKG	1 cut(s)	413
MspI	CCGG	8 cut(s)	241, 359, 487, 772, 816, 2703, 3082, 4073
MunI	CAATTG	3 cut(s)	1356, 2868, 3147
Mva1269I	GAATGC	2 cut(s)	324, 3007
MvnI	CGCG	1 cut(s)	2559
MwoI	GCNNNNNNNGC	8 cut(s)	295, 419, 1865, 2864, 3128, 3773, 3957, 5053
NciI	CCSGG	2 cut(s)	241, 773
NdeI	CATATG	1 cut(s)	2740
NheI	GCTAGC	2 cut(s)	623, 869
NsbI	TGCGCA	2 cut(s)	3482, 4329
NsiI	ATGCAT	1 cut(s)	2942
NspI	RCATGY	2 cut(s)	2940, 3456
OliI	CACNNNNGTG	2 cut(s)	2018, 2900
PagI	TCATGA	2 cut(s)	2758, 2791
PaqCI	CACCTGC	2 cut(s)	688, 5067
PasI	CCCWGGG	1 cut(s)	2963
PceI	AGGCCT	1 cut(s)	1505
PciI	ACATGT	1 cut(s)	3452
PciSI	GCTCTTC	2 cut(s)	1341, 3864
PctI	GAATGC	2 cut(s)	324, 3007
PdmI	GAANNNNTTC	3 cut(s)	397, 1630, 2955
PflMI	CCANNNNNTGG	1 cut(s)	2284
PinAI	ACCGGT	2 cut(s)	2702, 4072
PleI	GAGTC	8 cut(s)	349, 1430, 2605, 2662, 4222, 4599, 4645, 4901
PmaCI	CACGTG	1 cut(s)	4972
PmlI	CACGTG	1 cut(s)	4972
PpsI	GAGTC	8 cut(s)	349, 1430, 2605, 2662, 4222, 4599, 4645, 4901
Ppu21I	YACGTR	2 cut(s)	4526, 4972
PpuMI	RGGWCCY	3 cut(s)	46, 2174, 2346
PscI	ACATGT	1 cut(s)	3452
PshAI	GACNNNNGTC	1 cut(s)	4403
PshBI	ATTAAT	2 cut(s)	1592, 3999
PsiI	TTATAA	1 cut(s)	2055
Psp5II	RGGWCCY	3 cut(s)	46, 2174, 2346
PspCI	CACGTG	1 cut(s)	4972
PspFI	CCCAGC	1 cut(s)	2177
PspPPI	RGGWCCY	3 cut(s)	46, 2174, 2346
PstI	CTGCAG	4 cut(s)	346, 685, 2431, 4849
PstNI	CAGNNNCTG	2 cut(s)	89, 260
PvuII	CAGCTG	1 cut(s)	413
RseI	CAYNNNNRTG	4 cut(s)	2018, 2890, 2900, 3476
SapI	GCTCTTC	2 cut(s)	1341, 3864
ScaI	AGTACT	2 cut(s)	3406, 3750
SchI	GAGTC	8 cut(s)	349, 1430, 2605, 2662, 4222, 4600, 4646, 4902
SduI	GDGCHC	4 cut(s)	2534, 3103, 3208, 5094
SexAI	ACCWGGT	1 cut(s)	118
SfaNI	GCATC	7 cut(s)	1645, 2744, 2854, 4024, 4146, 4341, 4733
SfcI	CTRYAG	5 cut(s)	342, 681, 1977, 2427, 4845
SinI	GGWCC	8 cut(s)	46, 484, 2174, 2346, 3212, 3239, 4445, 4822
SmiMI	CAYNNNNRTG	4 cut(s)	2018, 2890, 2900, 3476
SmlI	CTYRAG	4 cut(s)	863, 1229, 3408, 4859
SmoI	CTYRAG	4 cut(s)	863, 1229, 3408, 4859
SpeI	ACTAGT	3 cut(s)	155, 689, 3823
SseBI	AGGCCT	1 cut(s)	1505
SspI	AATATT	4 cut(s)	951, 1843, 3041, 4320
StuI	AGGCCT	1 cut(s)	1505
StyI	CCWWGG	1 cut(s)	4466
TaiI	ACGT	6 cut(s)	748, 935, 3310, 4528, 4656, 4974
TaqII	GACCGA	2 cut(s)	184, 634
TauI	GCSGC	1 cut(s)	314
TseI	GCWGC	9 cut(s)	341, 413, 1175, 1283, 1430, 1542, 3128, 3667, 4330
Van91I	CCANNNNNTGG	1 cut(s)	2284
VpaK11BI	GGWCC	8 cut(s)	46, 484, 2174, 2346, 3212, 3239, 4445, 4822
VspI	ATTAAT	2 cut(s)	1592, 3999
XagI	CCTNNNNNAGG	1 cut(s)	1496
XbaI	TCTAGA	2 cut(s)	2614, 4357
XceI	RCATGY	2 cut(s)	2940, 3456
XmaJI	CCTAGG	1 cut(s)	4466
XmiI	GTMKAC	6 cut(s)	177, 929, 1671, 2252, 2495, 3514
XmnI	GAANNNNTTC	3 cut(s)	397, 1630, 2955
ZrmI	AGTACT	2 cut(s)	3406, 3750
Zsp2I	ATGCAT	1 cut(s)	2942

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.