Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0007430

Physical Location & Seq

Genomic Coordinates Reverse (-)

41936553 .. 41941145

Gene Structure 4593 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1286600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2310 bp

ATGGACATCAGCGAAGAAGTTAGGGCAAATCACCGCCGAGAGCTCCTTGCTTTCTTGGAAGATGGCATATACATGGACGAAATCAAGGCCGTCCTCAACCACAACCGCCGTCGCCTCATCGTCGACATCTCCGATCTCCACTCCTACGGCGAAATTGGCTCCAGGATTCTGAGAAATCCAAGTGATTATATGCAATCCTTCAACGACGCGGCCACTGAAATCGCCAACAGGATCGACCCGAGGTACCTGAAGCAAGGAGAACAACTTCTGGTGGGGTTTGAAGGTGCTTTCGTCTCGCGCCGTGTCACGCCGAGAGATCTGCTCTCCGAGTACATTGGTTCCATGGTTTGCGTCCAGGGCATTGTCACCAAATGTTCTCTTGTGAGGCCAAAAGTTGTTAAGAGTGTTCACTTCTGCCCTAGTACCGGAAACTTCACCTCTCGAGAATACCGAGACATTACATCCAATATGGGTTTGCCTACGGGAGCAGTTTATCCTACTAGGGACGAAAATGGTAACTTATTGGTGACTGAGTATGGACTATGCAAATACAAAGATCATCAAACTTTATCTATGCAAGAAGTTCCTGAGAACTCTGCTCCTGGTCAGCTTCCGCGGACAGTGGATGTCATAGTTGAGGATGACCTCGTTGATTCATGCAAACCAGGAGACCGTGTGGCGATTGTAGGGATTTACAAAGCTCTTCCTGGTAAAAGCAAGGGAAGCGTGAATGGAGTATTCAGGACTGTTCTCATAGCGAACAGCGTTTCGCTGCTCAACAAAGAAGCAAATGCACCAATTTACAGTCCTGATGACATAAAGAACATAAAAAAGATAGCAGAAAGAAGTGATACATTTGACCTGCTTGGGAATTCAATTGCACCCTCTATTTATGGACATTCATGGATAAAGAAAGCAGTAATATTGCTAATGCTTGGTGGAATGGAAAAGAACTTAAGGAATGGTACTCACTTACGAGGTGACATTAACATGATGATGGTTGGTGATCCTTCTGTTGCCAAATCTCAACTTCTAAGAGCAATTATGAACATCGCTCCATTGGCAATATCAACTACAGGCCGGGGTTCTTCTGGTGTTGGTTTGACAGCTGCTGTTACTTCGGATCAAGAGACGGGGGAGAGAAGGCTAGAAGCTGGTGCGATGGTTCTTGCTGATCGTGGTGTTGTCTGTATTGATGAATTTGACAAAATGAATGATCAAGATCGTGTTGCCATACATGAAGTTATGGAGCAGCAGACTGTAACAATTGCCAAAGCTGGTATCCATGCATCCCTCAATGCACGGTGCAGTGTGGTGGCTGCTGCAAATCCCATATATGGTTCTTATGATCGCTCATTGACACCAACAAAGAATATTGGACTTCCAGACTCTTTGCTCTCTCGTTTTGATTTACTGTTTATCGTATTGGATCAAATGGACCCTGACATTGACCGTCAAGTCTCAGAGCATGTGTTGCGCATGCATCGGTATCGTTCTGCAACTGGAGGTGAGGCAATGCTTGATGGGAGCTCGGCTTTTGGAAGAGAAGATGAAACTGATAATGACTCATCTGTGTTTGTTAAGTATAACCGAATGCTACATGGGAAAAAAACTGATAGGGGTCGAAAGCGTGATACGCTCACAATCAAGTTTCTTAAAAAGTATATTCATTATGCAAAGAATAGGATTGAGCCTGATCTGACTGATGAGGCCTCTGAACAAATTGCAACAGCGTATGCAGAACTCAGAAATGCCAGTTCAAATGCAAAGACTGGAGGAACACTTCCAATCACCGCCAGAACATTAGAAACCATCATTCGTCTCTCAACTGCTCATGCAAAATTAAAGTTGAGCAGAAAGGTTTCAAAGTCAGATGTTGACGCTGCTCTCAAAGTTTTAAATTTTGCAATTTATCACAAAGAATTGACTGACATGGAGGAGCGCGAGCAAGAAAGAGAGCGAGAATTGGAGAGGAACGGCAGAGCTAACCATCCCGCAGGTCAAGATAATGGGTCTGGCAATGGTACTGGGAATGAAGGTTCAACAACTGAACCCATGGATGTCGACCCTCACACTGAATCTGCTGCTGCTGATAACTCTTCCGAAAGGTTAAAAGCACTTACTGATGTTTTAAATCAAGTACGTACAGTACAGCGCTCCGACAGCATATCTGTTGAGGCCTTAGAGAATGCTGTCAATGACGGAGCAAGTGTTCCTTACTCAAGTACAGAGATAACCTTCCTATTAGAGGAACTCCGAAAGAGAAACATAGTGATGGTAGATGGTGGAACAGTGTTCATAATATCATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000347 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006268 GO:0006725 GO:0006807 GO:0006996 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0016043 GO:0032392 GO:0032508 GO:0032991 GO:0034641 GO:0034645 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0046483 GO:0051276 GO:0071103 GO:0071704 GO:0071840 GO:0090304 GO:1901360 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko03030 ko04110 ko04111 ko04113 map03030 map04110 map04111 map04113

Pfam Domains

Protein Families

MCM MCM_N MCM_OB

Protein Analysis

770

Amino Acids

84.77

Weight (kDa)

5.95

Isoelectric Point (pI)

34.62

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MCM_N	PF14551	19 - 94	1.4e-10	MCM N-terminal domain
MCM_OB	PF17207	105 - 235	7.6e-31	MCM OB domain
MCM	PF00493	272 - 495	1.8e-96	MCM P-loop domain
Mg_chelatase	PF01078	379 - 446	3.5e-07	Magnesium chelatase, subunit ChlI
MCM_lid	PF17855	551 - 634	4.1e-26	MCM AAA-lid domain
WHD_MCM3_C	PF23191	700 - 768	1.5e-09	MCM3-like, winged helix domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0007430)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G46280 AT5G46280 AT5G46280 AT5G46280
fragaria_vesca	FvH4_3g05860
malus_domestica	MD05G1286600.v1.1 MD10G1264100.v1.1
prunus_persica	Prupe.4G078300_v2.0.a1
pyrus_communis	pycom10g22020
rosa_chinensis	RchiOBHm_Chr5g0013601 RchiOBHm_Chr5g0013661
rosa_laevigata	RLG00000032038
rosa_multiflora	Rmu_sc0002573.1_g000006 Rmu_sc0002573.1_g000015
rosa_roxburghii	Rroxscaffold_1G00062940
rosa_rugosa	Rorug05G0011700 Rorug05G0011800
rosa_samantha	Rh5AG106100 Rh5AG106300 Rh5BG102500 Rh5CG114000 Rh5CG114400 Rh5DG100800 Rh5DG101200
rosa_wichuraiana	Rw5G009270

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1457
Acc16I	TGCGCA	1 cut(s)	1478
Acc36I	ACCTGC	2 cut(s)	870, 1988
Acc65I	GGTACC	1 cut(s)	243
AccB1I	GGYRCC	1 cut(s)	243
AccI	GTMKAC	2 cut(s)	123, 2064
AccII	CGCG	4 cut(s)	209, 298, 616, 1944
AciI	CCGC	7 cut(s)	34, 106, 209, 614, 616, 1794, 1995
AclWI	GGATC	4 cut(s)	239, 1001, 1131, 1437
AcoI	YGGCCR	1 cut(s)	210
AcsI	RAATTY	3 cut(s)	871, 1199, 1900
AcuI	CTGAAG	1 cut(s)	269
AfaI	GTAC	9 cut(s)	245, 332, 424, 967, 2026, 2142, 2146, 2151, 2227
AfeI	AGCGCT	1 cut(s)	2156
AfiI	CCNNNNNNNGG	3 cut(s)	425, 1337, 2248
AflII	CTTAAG	1 cut(s)	955
AgsI	TTSAA	6 cut(s)	202, 281, 876, 1761, 1866, 2043
AjnI	CCWGG	5 cut(s)	161, 354, 601, 664, 706
AloI	GAACNNNNNNTCC	4 cut(s)	2023, 2055, 2196, 2228
AluBI	AGCT	8 cut(s)	43, 610, 701, 1109, 1154, 1277, 1530, 1985
AluI	AGCT	8 cut(s)	43, 610, 701, 1109, 1154, 1277, 1530, 1985
Alw21I	GWGCWC	2 cut(s)	45, 1532
Alw26I	GTCTC	6 cut(s)	298, 447, 663, 1124, 1465, 1826
AlwI	GGATC	4 cut(s)	239, 1001, 1131, 1437
AlwNI	CAGNNNCTG	1 cut(s)	1112
Ama87I	CYCGRG	2 cut(s)	238, 441
Aor51HI	AGCGCT	1 cut(s)	2156
AoxI	GGCC	6 cut(s)	87, 210, 386, 1078, 1710, 2178
ApeKI	GCWGC	8 cut(s)	772, 1109, 1252, 1319, 1322, 1883, 2084, 2087
ApoI	RAATTY	3 cut(s)	871, 1199, 1900
Asp700I	GAANNNNTTC	2 cut(s)	264, 1861
Asp718I	GGTACC	1 cut(s)	243
AspLEI	GCGC	4 cut(s)	300, 1479, 1944, 2157
AspS9I	GGNCC	1 cut(s)	1438
AsuC2I	CCSGG	1 cut(s)	1082
AsuHPI	GGTGA	8 cut(s)	23, 358, 427, 538, 992, 1016, 1520, 1783
AvaI	CYCGRG	2 cut(s)	238, 441
AvaII	GGWCC	1 cut(s)	1438
BanI	GGYRCC	1 cut(s)	243
BanII	GRGCYC	2 cut(s)	45, 1532
Bbv12I	GWGCWC	2 cut(s)	45, 1532
BbvI	GCAGC	8 cut(s)	759, 1096, 1264, 1306, 1309, 1870, 2071, 2074
BccI	CCATC	8 cut(s)	56, 991, 1156, 1517, 1820, 1998, 2269, 2276
BceAI	ACGGC	5 cut(s)	74, 93, 163, 285, 1993
BcgI	CGANNNNNNTGC	4 cut(s)	301, 335, 1472, 1506
BciT130I	CCWGG	5 cut(s)	163, 356, 603, 666, 708
BciVI	GTATCC	1 cut(s)	1292
BclI	TGATCA	1 cut(s)	1216
BcnI	CCSGG	1 cut(s)	1082
BcoDI	GTCTC	6 cut(s)	298, 447, 663, 1124, 1465, 1826
BfaI	CTAG	3 cut(s)	420, 501, 1148
BfmI	CTRYAG	1 cut(s)	1074
BfoI	RGCGCY	1 cut(s)	2158
BfrI	CTTAAG	1 cut(s)	955
BfuAI	ACCTGC	2 cut(s)	870, 1988
BfuI	GTATCC	1 cut(s)	1292
BglII	AGATCT	1 cut(s)	316
BisI	GCNGC	9 cut(s)	210, 773, 1110, 1253, 1320, 1323, 1884, 2085, 2088
BlsI	GCNGC	9 cut(s)	211, 774, 1111, 1254, 1321, 1324, 1885, 2086, 2089
Bme1390I	CCNGG	6 cut(s)	163, 356, 603, 666, 708, 1082
Bme18I	GGWCC	1 cut(s)	1438
BmeT110I	CYCGRG	2 cut(s)	238, 441
BmgT120I	GGNCC	1 cut(s)	1438
BmiI	GGNNCC	4 cut(s)	160, 245, 340, 1440
BmrFI	CCNGG	6 cut(s)	163, 356, 603, 666, 708, 1082
BmrI	ACTGGG	1 cut(s)	2037
BmsI	GCATC	2 cut(s)	1298, 1492
BmuI	ACTGGG	1 cut(s)	2037
BplI	GAGNNNNNCTC	2 cut(s)	306, 338
BpmI	CTGGAG	3 cut(s)	145, 1524, 1794
BpuEI	CTTGAG	1 cut(s)	2206
BpuMI	CCSGG	1 cut(s)	1082
BsaAI	YACGTR	1 cut(s)	2144
BsaBI	GATNNNNATC	1 cut(s)	1221
BsaI	GGTCTC	1 cut(s)	663
BsaJI	CCNNGG	6 cut(s)	239, 342, 355, 614, 1081, 2055
BsaWI	WCCGGW	1 cut(s)	425
BsaXI	ACNNNNNCTCC	4 cut(s)	660, 690, 2196, 2226
Bsc4I	CCNNNNNNNGG	3 cut(s)	425, 1337, 2248
Bse1I	ACTGG	4 cut(s)	1507, 1755, 1777, 2032
Bse3DI	GCAATG	2 cut(s)	1521, 2026
Bse8I	GATNNNNATC	1 cut(s)	1221
BseBI	CCWGG	5 cut(s)	163, 356, 603, 666, 708
BseDI	CCNNGG	6 cut(s)	239, 342, 355, 614, 1081, 2055
BseGI	GGATG	6 cut(s)	461, 631, 646, 1289, 1990, 2065
BseJI	GATNNNNATC	1 cut(s)	1221
BseLI	CCNNNNNNNGG	3 cut(s)	425, 1337, 2248
BseMI	GCAATG	2 cut(s)	1521, 2026
BseMII	CTCAG	5 cut(s)	161, 522, 579, 1476, 1759
BseNI	ACTGG	4 cut(s)	1507, 1755, 1777, 2032
BseRI	GAGGAG	1 cut(s)	1952
BseXI	GCAGC	8 cut(s)	759, 1096, 1264, 1306, 1309, 1870, 2071, 2074
BsgI	GTGCAG	1 cut(s)	1327
Bsh1236I	CGCG	4 cut(s)	209, 298, 616, 1944
BshFI	GGCC	6 cut(s)	89, 212, 388, 1080, 1712, 2180
BshNI	GGYRCC	1 cut(s)	243
BsiHKAI	GWGCWC	2 cut(s)	45, 1532
BsiHKCI	CYCGRG	2 cut(s)	238, 441
BsiSI	CCGG	2 cut(s)	426, 1081
BslFI	GGGAC	1 cut(s)	518
BslI	CCNNNNNNNGG	3 cut(s)	425, 1337, 2248
BsmAI	GTCTC	6 cut(s)	298, 447, 663, 1124, 1465, 1826
BsmBI	CGTCTC	3 cut(s)	298, 1124, 1826
BsmFI	GGGAC	1 cut(s)	518
BsmI	GAATGC	2 cut(s)	1599, 2194
BsnI	GGCC	6 cut(s)	89, 212, 388, 1080, 1712, 2180
Bso31I	GGTCTC	1 cut(s)	663
BsoBI	CYCGRG	2 cut(s)	238, 441
Bsp1286I	GDGCHC	2 cut(s)	45, 1532
Bsp19I	CCATGG	2 cut(s)	342, 2055
BspACI	CCGC	7 cut(s)	34, 106, 209, 614, 616, 1794, 1995
BspANI	GGCC	6 cut(s)	89, 212, 388, 1080, 1712, 2180
BspCNI	CTCAG	5 cut(s)	162, 523, 580, 1475, 1758
BspFNI	CGCG	4 cut(s)	209, 298, 616, 1944
BspHI	TCATGA	1 cut(s)	2306
BspLI	GGNNCC	4 cut(s)	160, 245, 340, 1440
BspMI	ACCTGC	2 cut(s)	870, 1988
BspPI	GGATC	4 cut(s)	239, 1001, 1131, 1437
BspQI	GCTCTTC	1 cut(s)	708
BspT107I	GGYRCC	1 cut(s)	243
BspTI	CTTAAG	1 cut(s)	955
BspTNI	GGTCTC	1 cut(s)	663
BsrDI	GCAATG	2 cut(s)	1521, 2026
BsrI	ACTGG	4 cut(s)	1507, 1755, 1777, 2032
BssECI	CCNNGG	6 cut(s)	239, 342, 355, 614, 1081, 2055
BssT1I	CCWWGG	2 cut(s)	342, 2055
Bst2UI	CCWGG	5 cut(s)	163, 356, 603, 666, 708
Bst6I	CTCTTC	3 cut(s)	708, 1537, 2104
BstAFI	CTTAAG	1 cut(s)	955
BstAPI	GCANNNNNTGC	1 cut(s)	1474
BstBAI	YACGTR	1 cut(s)	2144
BstC8I	GCNNGC	2 cut(s)	1481, 1946
BstDEI	CTNAG	7 cut(s)	170, 531, 588, 1034, 1462, 1745, 2182
BstDSI	CCRYGG	3 cut(s)	342, 614, 2055
BstENI	CCTNNNNNAGG	1 cut(s)	2246
BstF5I	GGATG	6 cut(s)	461, 631, 646, 1289, 1990, 2065
BstFNI	CGCG	4 cut(s)	209, 298, 616, 1944
BstH2I	RGCGCY	1 cut(s)	2158
BstHHI	GCGC	4 cut(s)	300, 1479, 1944, 2157
BstMAI	GTCTC	6 cut(s)	298, 447, 663, 1124, 1465, 1826
BstMWI	GCNNNNNNNGC	7 cut(s)	156, 357, 723, 1061, 1474, 1636, 2163
BstNI	CCWGG	5 cut(s)	163, 356, 603, 666, 708
BstNSI	RCATGY	2 cut(s)	1472, 1483
BstSCI	CCNGG	6 cut(s)	161, 354, 601, 664, 706, 1080
BstSFI	CTRYAG	1 cut(s)	1074
BstSNI	TACGTA	1 cut(s)	2144
BstUI	CGCG	4 cut(s)	209, 298, 616, 1944
BstV1I	GCAGC	8 cut(s)	759, 1096, 1264, 1306, 1309, 1870, 2071, 2074
BstX2I	RGATCY	1 cut(s)	316
BstYI	RGATCY	1 cut(s)	316
BsuI	GTATCC	1 cut(s)	1292
BsuRI	GGCC	6 cut(s)	89, 212, 388, 1080, 1712, 2180
BtgI	CCRYGG	3 cut(s)	342, 614, 2055
BtgZI	GCGATG	2 cut(s)	1036, 1175
BtsCI	GGATG	6 cut(s)	461, 631, 646, 1289, 1990, 2065
BtsI	GCAGTG	1 cut(s)	1315
BtsIMutI	CAGTG	5 cut(s)	213, 627, 1315, 2073, 2298
BveI	ACCTGC	2 cut(s)	870, 1988
Cac8I	GCNNGC	2 cut(s)	1481, 1946
CaiI	CAGNNNCTG	1 cut(s)	1112
CciI	TCATGA	1 cut(s)	2306
CfoI	GCGC	4 cut(s)	300, 1479, 1944, 2157
Cfr13I	GGNCC	1 cut(s)	1438
Cfr42I	CCGCGG	1 cut(s)	617
CseI	GACGC	3 cut(s)	215, 340, 1889
Csp6I	GTAC	9 cut(s)	244, 331, 423, 966, 2025, 2141, 2145, 2150, 2226
CviQI	GTAC	9 cut(s)	244, 331, 423, 966, 2025, 2141, 2145, 2150, 2226
DdeI	CTNAG	7 cut(s)	170, 531, 588, 1034, 1462, 1745, 2182
DraI	TTTAAA	2 cut(s)	1899, 2133
DrdI	GACNNNNNNGTC	1 cut(s)	1457
DseDI	GACNNNNNNGTC	1 cut(s)	1457
EaeI	YGGCCR	1 cut(s)	210
Eam1104I	CTCTTC	3 cut(s)	708, 1537, 2104
EarI	CTCTTC	3 cut(s)	708, 1537, 2104
Ecl136II	GAGCTC	2 cut(s)	43, 1530
Eco105I	TACGTA	1 cut(s)	2144
Eco130I	CCWWGG	2 cut(s)	342, 2055
Eco147I	AGGCCT	2 cut(s)	1712, 2180
Eco24I	GRGCYC	2 cut(s)	45, 1532
Eco31I	GGTCTC	1 cut(s)	663
Eco47I	GGWCC	1 cut(s)	1438
Eco47III	AGCGCT	1 cut(s)	2156
Eco53kI	GAGCTC	2 cut(s)	43, 1530
Eco57I	CTGAAG	1 cut(s)	269
Eco88I	CYCGRG	2 cut(s)	238, 441
EcoICRI	GAGCTC	2 cut(s)	43, 1530
EcoNI	CCTNNNNNAGG	1 cut(s)	2246
EcoRI	GAATTC	1 cut(s)	871
EcoRII	CCWGG	5 cut(s)	161, 354, 601, 664, 706
EcoT14I	CCWWGG	2 cut(s)	342, 2055
EcoT22I	ATGCAT	2 cut(s)	1291, 1485
EcoT38I	GRGCYC	2 cut(s)	45, 1532
ErhI	CCWWGG	2 cut(s)	342, 2055
Esp3I	CGTCTC	3 cut(s)	298, 1124, 1826
FalI	AAGNNNNNCTT	2 cut(s)	2200, 2232
FaqI	GGGAC	1 cut(s)	518
FauI	CCCGC	1 cut(s)	2002
FbaI	TGATCA	1 cut(s)	1216
FblI	GTMKAC	2 cut(s)	123, 2064
Fnu4HI	GCNGC	9 cut(s)	210, 773, 1110, 1253, 1320, 1323, 1884, 2085, 2088
FokI	GGATG	6 cut(s)	448, 638, 653, 1276, 1977, 2072
FriOI	GRGCYC	2 cut(s)	45, 1532
Fsp4HI	GCNGC	9 cut(s)	210, 773, 1110, 1253, 1320, 1323, 1884, 2085, 2088
FspBI	CTAG	3 cut(s)	420, 501, 1148
FspI	TGCGCA	1 cut(s)	1478
GlaI	GCGC	4 cut(s)	299, 1478, 1943, 2156
GluI	GCNGC	9 cut(s)	210, 773, 1110, 1253, 1320, 1323, 1884, 2085, 2088
GsuI	CTGGAG	3 cut(s)	145, 1524, 1794
HaeII	RGCGCY	1 cut(s)	2158
HaeIII	GGCC	6 cut(s)	89, 212, 388, 1080, 1712, 2180
HapII	CCGG	2 cut(s)	426, 1081
HgaI	GACGC	3 cut(s)	215, 340, 1889
HhaI	GCGC	4 cut(s)	300, 1479, 1944, 2157
Hin6I	GCGC	4 cut(s)	298, 1477, 1942, 2155
HinP1I	GCGC	4 cut(s)	298, 1477, 1942, 2155
HincII	GTYRAC	3 cut(s)	124, 1879, 2065
HindII	GTYRAC	3 cut(s)	124, 1879, 2065
HinfI	GANTC	5 cut(s)	166, 653, 1388, 1565, 2078
HpaII	CCGG	2 cut(s)	426, 1081
HphI	GGTGA	8 cut(s)	23, 358, 427, 538, 992, 1016, 1520, 1783
Hpy166II	GTNNAC	4 cut(s)	124, 409, 1879, 2065
Hpy8I	GTNNAC	4 cut(s)	124, 409, 1879, 2065
Hpy99I	CGWCG	3 cut(s)	114, 125, 209
HpyAV	CCTTC	6 cut(s)	208, 275, 1020, 1137, 2030, 2248
HpyCH4IV	ACGT	1 cut(s)	2143
HpyF10VI	GCNNNNNNNGC	7 cut(s)	156, 357, 723, 1061, 1474, 1636, 2163
HpyF3I	CTNAG	7 cut(s)	170, 531, 588, 1034, 1462, 1745, 2182
HpySE526I	ACGT	1 cut(s)	2143
HspAI	GCGC	4 cut(s)	298, 1477, 1942, 2155
KpnI	GGTACC	1 cut(s)	247
Ksp22I	TGATCA	1 cut(s)	1216
KspI	CCGCGG	1 cut(s)	617
LguI	GCTCTTC	1 cut(s)	708
Lsp1109I	GCAGC	8 cut(s)	759, 1096, 1264, 1306, 1309, 1870, 2071, 2074
LweI	GCATC	2 cut(s)	1298, 1492
MaeI	CTAG	3 cut(s)	420, 501, 1148
MaeII	ACGT	1 cut(s)	2143
MaeIII	GTNAC	7 cut(s)	304, 364, 515, 526, 980, 1114, 1261
MboII	GAAGA	7 cut(s)	26, 71, 695, 1080, 1554, 1559, 2091
MfeI	CAATTG	2 cut(s)	876, 1266
MflI	RGATCY	1 cut(s)	316
MhlI	GDGCHC	2 cut(s)	45, 1532
MlyI	GAGTC	2 cut(s)	1382, 1559
MmeI	TCCRAC	1 cut(s)	2184
Mph1103I	ATGCAT	2 cut(s)	1291, 1485
MroXI	GAANNNNTTC	2 cut(s)	264, 1861
MseI	TTAA	9 cut(s)	399, 956, 987, 1581, 1656, 1844, 1898, 2111, 2132
MslI	CAYNNNNRTG	4 cut(s)	71, 989, 995, 2273
MspA1I	CMGCKG	2 cut(s)	616, 1109
MspCI	CTTAAG	1 cut(s)	955
MspI	CCGG	2 cut(s)	426, 1081
MspR9I	CCNGG	6 cut(s)	163, 356, 603, 666, 708, 1082
MunI	CAATTG	2 cut(s)	876, 1266
Mva1269I	GAATGC	2 cut(s)	1599, 2194
MvaI	CCWGG	5 cut(s)	163, 356, 603, 666, 708
MvnI	CGCG	4 cut(s)	209, 298, 616, 1944
MwoI	GCNNNNNNNGC	7 cut(s)	156, 357, 723, 1061, 1474, 1636, 2163
NciI	CCSGG	1 cut(s)	1082
NcoI	CCATGG	2 cut(s)	342, 2055
NlaIV	GGNNCC	4 cut(s)	160, 245, 340, 1440
NmeAIII	GCCGAG	3 cut(s)	62, 336, 1511
NmuCI	GTSAC	4 cut(s)	304, 364, 526, 980
NsbI	TGCGCA	1 cut(s)	1478
NsiI	ATGCAT	2 cut(s)	1291, 1485
NspI	RCATGY	2 cut(s)	1472, 1483
PaeI	GCATGC	1 cut(s)	1483
PaeR7I	CTCGAG	1 cut(s)	441
PagI	TCATGA	1 cut(s)	2306
PceI	AGGCCT	2 cut(s)	1712, 2180
PciSI	GCTCTTC	1 cut(s)	708
PcsI	WCGNNNNNNNCGW	2 cut(s)	129, 448
PctI	GAATGC	2 cut(s)	1599, 2194
PdmI	GAANNNNTTC	2 cut(s)	264, 1861
PfeI	GAWTC	3 cut(s)	166, 653, 2078
PfoI	TCCNGGA	1 cut(s)	161
PkrI	GCNGC	9 cut(s)	211, 774, 1111, 1254, 1321, 1324, 1885, 2086, 2089
PleI	GAGTC	2 cut(s)	1382, 1559
PpsI	GAGTC	2 cut(s)	1382, 1559
Ppu21I	YACGTR	1 cut(s)	2144
Psp124BI	GAGCTC	2 cut(s)	45, 1532
Psp6I	CCWGG	5 cut(s)	161, 354, 601, 664, 706
PspGI	CCWGG	5 cut(s)	161, 354, 601, 664, 706
PspN4I	GGNNCC	4 cut(s)	160, 245, 340, 1440
PspPI	GGNCC	1 cut(s)	1438
PstNI	CAGNNNCTG	1 cut(s)	1112
PsuI	RGATCY	1 cut(s)	316
PvuII	CAGCTG	1 cut(s)	1109
RsaI	GTAC	9 cut(s)	245, 332, 424, 967, 2026, 2142, 2146, 2151, 2227
RsaNI	GTAC	9 cut(s)	244, 331, 423, 966, 2025, 2141, 2145, 2150, 2226
RseI	CAYNNNNRTG	4 cut(s)	71, 989, 995, 2273
SacI	GAGCTC	2 cut(s)	45, 1532
SacII	CCGCGG	1 cut(s)	617
SalI	GTCGAC	2 cut(s)	122, 2063
SapI	GCTCTTC	1 cut(s)	708
SaqAI	TTAA	9 cut(s)	399, 956, 987, 1581, 1656, 1844, 1898, 2111, 2132
SatI	GCNGC	9 cut(s)	210, 773, 1110, 1253, 1320, 1323, 1884, 2085, 2088
Sau96I	GGNCC	1 cut(s)	1438
SchI	GAGTC	2 cut(s)	1382, 1559
ScrFI	CCNGG	6 cut(s)	163, 356, 603, 666, 708, 1082
SduI	GDGCHC	2 cut(s)	45, 1532
SfaNI	GCATC	2 cut(s)	1298, 1492
SfcI	CTRYAG	1 cut(s)	1074
Sfr274I	CTCGAG	1 cut(s)	441
Sfr303I	CCGCGG	1 cut(s)	617
SgrBI	CCGCGG	1 cut(s)	617
SinI	GGWCC	1 cut(s)	1438
SlaI	CTCGAG	1 cut(s)	441
SmiMI	CAYNNNNRTG	4 cut(s)	71, 989, 995, 2273
SmlI	CTYRAG	3 cut(s)	441, 955, 2221
SmoI	CTYRAG	3 cut(s)	441, 955, 2221
SnaBI	TACGTA	1 cut(s)	2144
SphI	GCATGC	1 cut(s)	1483
SseBI	AGGCCT	2 cut(s)	1712, 2180
SsiI	CCGC	7 cut(s)	34, 106, 209, 614, 616, 1794, 1995
SspI	AATATT	2 cut(s)	924, 1375
SspMI	CTAG	3 cut(s)	420, 501, 1148
SstI	GAGCTC	2 cut(s)	45, 1532
StuI	AGGCCT	2 cut(s)	1712, 2180
StyD4I	CCNGG	6 cut(s)	161, 354, 601, 664, 706, 1080
StyI	CCWWGG	2 cut(s)	342, 2055
TaiI	ACGT	1 cut(s)	2146
TaqI	TCGA	5 cut(s)	123, 234, 442, 1624, 2064
TatI	WGTACW	3 cut(s)	330, 2149, 2225
TauI	GCSGC	1 cut(s)	212
TfiI	GAWTC	3 cut(s)	166, 653, 2078
Tru1I	TTAA	9 cut(s)	399, 956, 987, 1581, 1656, 1844, 1898, 2111, 2132
Tru9I	TTAA	9 cut(s)	399, 956, 987, 1581, 1656, 1844, 1898, 2111, 2132
TscAI	CASTG	5 cut(s)	220, 627, 1315, 2080, 2298
TseFI	GTSAC	4 cut(s)	304, 364, 526, 980
TseI	GCWGC	8 cut(s)	772, 1109, 1252, 1319, 1322, 1883, 2084, 2087
Tsp45I	GTSAC	4 cut(s)	304, 364, 526, 980
TspGWI	ACGGA	1 cut(s)	2217
TspRI	CASTG	5 cut(s)	220, 627, 1315, 2080, 2298
Vha464I	CTTAAG	1 cut(s)	955
VpaK11BI	GGWCC	1 cut(s)	1438
XagI	CCTNNNNNAGG	1 cut(s)	2246
XapI	RAATTY	3 cut(s)	871, 1199, 1900
XceI	RCATGY	2 cut(s)	1472, 1483
XhoI	CTCGAG	1 cut(s)	441
XmiI	GTMKAC	2 cut(s)	123, 2064
XmnI	GAANNNNTTC	2 cut(s)	264, 1861
XspI	CTAG	3 cut(s)	420, 501, 1148
Zsp2I	ATGCAT	2 cut(s)	1291, 1485

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.