Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0007430

Physical Location & Seq

Genomic Coordinates Reverse (-)

35780556 .. 35785798

Gene Structure 5243 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1264100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2340 bp

ATGGACGTCAGCGAAGAAGTTAGGGCAGTTCACCGGCGAGATCTCCTTGCTTTCTTGGAAGATGGCATATACATGGACGAAATCAAGGCCGTCCTCAACCACAACCGCCGCCGCCTCATTGTCGACATCTCCGATCTCCACTCCTACGGTGACATTGGTTCCAGGATTCTGAGAAATCCAAGTGATTATATGCAATCCTTCAACGACGCGGCCACTGAAATCGCCAACAGGATCGACCCGAGGTACCTGAAGCAAGGAGAACAACTTCTGGTGGGGTTTGAAGGCGCTTTTGTCTCGCGCCGTGTCACACCGAGAGATCTGCTCTCCGAGTACATTGGTTCCATGGTCTGCGTCCAAGGCATTGTCACCAAATGTTCACTTGTGAGGCCAAAAGTTGTTAAGAGTGTTCACTTCTGCCCTACTACTGGAAACTTCACCTCTCGAGAATACCGAGACATTACATCCAACATGGGTTTGCCTACAGGATCAGTTTATCCTACAAGTGATGAAAATGGTAACTTGTTGGTGACTGAGTACGGACTGTGCAAATACAAAGACCATCAAACTTTATCCATGCAAGAAGTTCCTGAGAACTCTGCTCCTGGTCAGCTTCCGCGGACAGTGGATGTCATAGTTGAGGATGACCTTGTTGATTCATGCAAACCAGGAGACCGTGTTGCGATTGTAGGGATATACAAAGCTCTTCCTGGTAAAAGCAAGGGAAGCGTAAATGGAGTATTCAGGACTGTTCTCATAGCGAACAATGTTTCGCTGCTCAACAAAGAAGCAAATGCACCAATTTACAGTCCTGATGACATAAAGAACATAAAAAAGATAGCAGAAAGAAGTGATACGTTTGACCTGCTTGGGAATTCAATTGCACCCTCTATTTATGGACATTCATGGATAAAGAAAGCAGTAATATTACTAATGCTTGGTGGAATGGAAAAGAACTTAAAGAATGGTACTCACTTACGAGGTGACATTAACATGATGATGGTTGGTGATCCGTCTGTTGCAAAATCTCAACTTCTAAGAGCAATTATGAACATTGCTCCCTTGGCAATATCAACTACAGGCCGGGGTTCTTCTGGTGTTGGCTTGACAGCTGCTGTTACTTCGGATCAAGAGACAGGGGAGAGAAGGCTAGAAGCTGGTGCGATGGTTCTTGCTGATCGTGGTGTTGTCTGTATTGATGAATTTGACAAAATGAATGATCAAGATCGTGTTGCCATACATGAAGTTATGGAGCAGCAGACTGTAACTATTGCCAAAGCTGGTATCCATGCATCCCTCAATGCACGGTGCAGTGTGGTGGCTGCTGCAAATCCCATATATGGTTCTTATGATCGCTCGTTGACACCAACAAAGAATATTGGACTTCCAGACTCTCTGCTTTCTCGTTTTGATTTACTGTTTATCGTATTGGATCAAATGGATCCTGACATTGACCGTCATGTCTCAGAGCATGTGTTGCGCATGCATCGGTATCGTTCTGCAACTGGAGGTGAGGCAATGCTTGATGGGAGCTCGGCATTTGGAAGAGAAGATGAAACTAATAACGACTCATCTGTATTTGTTAAGTATAACCGAATGCTACATGGGAAAAAAACCGATAGGGGTCGGAAGCGTGATACGCTCACAATCAAGTTTCTTAAAAAGTATATTCATTATGCAAAGAATAGGATTGAGCCTGATCTGACTGATGAGGCCTCTGAACAAATTGCAACAACATATGCAGAACTCAGAAATGCCAGTTCAAATGCAAAGACTGGAGGAACACTTCCAATTACAGCCAGAACTTTAGAAACCATCATTCGTCTCTCAACTGCTCATGCAAAATTAAAGTTGAGCAGAAAGGTTTCAAAGTCTGATGTTGACGCTGCTCTTAAAGTTTTAAATTTTGCAATATATCACAAAGAGTTGACTGACATGGAGGAGCGGGAGCAAGAAAGAGAGCGAGAATTGGAGAGGAATGGCAGACCTAACCATTCCGCAGGTCAGGAAGGAGCTGACCATTCCGCGGGTCAACATGATGGGTCTGGCAATGGTACTACTAATAATCAAGATTCAACAACTGAACCCATGGATGTTGACCCTCGCACTGAATCTGCTGCTACTAATAACTCTTCCGAAAGGTTAAAAGCACTTACTGATGTTTTAAATCAAGTACGGACGGTACAGCGCGCGGAAAGCATATCCGTTGAGGCCTTAGAGAATGCTGTCAACGACGGAGCAAGTGTTCCTTACTCAAGGACAGAGATAACCTTCCTATTAGAGGAACTCCGAAGGAGAAACATAGTGATGGTAGATGGTGGAACAGTGTTCATAATATCGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000347 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006268 GO:0006725 GO:0006807 GO:0006996 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0016043 GO:0032392 GO:0032508 GO:0032991 GO:0034641 GO:0034645 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0046483 GO:0051276 GO:0071103 GO:0071704 GO:0071840 GO:0090304 GO:1901360 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko03030 ko04110 ko04111 ko04113 map03030 map04110 map04111 map04113

Pfam Domains

Protein Families

MCM MCM_N MCM_OB

Protein Analysis

780

Amino Acids

85.96

Weight (kDa)

6.08

Isoelectric Point (pI)

34.24

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MCM_N	PF14551	21 - 94	1.1e-10	MCM N-terminal domain
MCM_OB	PF17207	105 - 235	5.3e-31	MCM OB domain
MCM	PF00493	272 - 495	1.6e-96	MCM P-loop domain
Mg_chelatase	PF01078	379 - 446	3.6e-07	Magnesium chelatase, subunit ChlI
MCM_lid	PF17855	551 - 634	6.1e-26	MCM AAA-lid domain
WHD_MCM3_C	PF23191	710 - 778	1.7e-09	MCM3-like, winged helix domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007430)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G46280 AT5G46280 AT5G46280 AT5G46280
fragaria_vesca	FvH4_3g05860
malus_domestica	MD05G1286600.v1.1 MD10G1264100.v1.1
prunus_persica	Prupe.4G078300_v2.0.a1
pyrus_communis	pycom10g22020
rosa_chinensis	RchiOBHm_Chr5g0013601 RchiOBHm_Chr5g0013661
rosa_laevigata	RLG00000032038
rosa_multiflora	Rmu_sc0002573.1_g000006 Rmu_sc0002573.1_g000015
rosa_roxburghii	Rroxscaffold_1G00062940
rosa_rugosa	Rorug05G0011700 Rorug05G0011800
rosa_samantha	Rh5AG106100 Rh5AG106300 Rh5BG102500 Rh5CG114000 Rh5CG114400 Rh5DG100800 Rh5DG101200
rosa_wichuraiana	Rw5G009270

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1457
AatII	GACGTC	1 cut(s)	9
Acc16I	TGCGCA	1 cut(s)	1478
Acc36I	ACCTGC	2 cut(s)	870, 1988
Acc65I	GGTACC	1 cut(s)	243
AccB1I	GGYRCC	1 cut(s)	243
AccBSI	CCGCTC	1 cut(s)	1942
AccI	GTMKAC	1 cut(s)	123
AccII	CGCG	6 cut(s)	209, 298, 616, 2024, 2187, 2189
AclWI	GGATC	7 cut(s)	239, 493, 1001, 1131, 1433, 1437, 1446
AcoI	YGGCCR	1 cut(s)	210
AcsI	RAATTY	3 cut(s)	871, 1199, 1900
AcuI	CTGAAG	1 cut(s)	269
AcyI	GRCGYC	1 cut(s)	6
AfaI	GTAC	7 cut(s)	245, 332, 536, 967, 2053, 2172, 2181
AfiI	CCNNNNNNNGG	4 cut(s)	425, 1337, 2023, 2278
AgsI	TTSAA	6 cut(s)	202, 281, 876, 1761, 1866, 2073
AjnI	CCWGG	4 cut(s)	161, 601, 664, 706
AloI	GAACNNNNNNTCC	2 cut(s)	2226, 2258
AluBI	AGCT	7 cut(s)	610, 701, 1109, 1154, 1277, 1530, 2012
AluI	AGCT	7 cut(s)	610, 701, 1109, 1154, 1277, 1530, 2012
Alw21I	GWGCWC	1 cut(s)	1532
Alw26I	GTCTC	6 cut(s)	298, 447, 663, 1124, 1465, 1826
AlwI	GGATC	7 cut(s)	239, 493, 1001, 1131, 1433, 1437, 1446
AlwNI	CAGNNNCTG	1 cut(s)	1112
Ama87I	CYCGRG	2 cut(s)	238, 441
AoxI	GGCC	6 cut(s)	87, 210, 386, 1078, 1710, 2208
ApeKI	GCWGC	7 cut(s)	772, 1109, 1252, 1319, 1322, 1883, 2114
ApoI	RAATTY	3 cut(s)	871, 1199, 1900
Asp700I	GAANNNNTTC	2 cut(s)	264, 1861
Asp718I	GGTACC	1 cut(s)	243
AspLEI	GCGC	5 cut(s)	287, 300, 1479, 2187, 2189
AsuC2I	CCSGG	1 cut(s)	1082
AsuHPI	GGTGA	8 cut(s)	23, 161, 358, 427, 538, 992, 1016, 1520
AvaI	CYCGRG	2 cut(s)	238, 441
BamHI	GGATCC	1 cut(s)	1438
BanI	GGYRCC	1 cut(s)	243
BanII	GRGCYC	1 cut(s)	1532
Bbv12I	GWGCWC	1 cut(s)	1532
BbvI	GCAGC	7 cut(s)	759, 1096, 1264, 1306, 1309, 1870, 2101
BccI	CCATC	9 cut(s)	56, 567, 991, 1156, 1517, 1820, 2030, 2299, 2306
BceAI	ACGGC	2 cut(s)	74, 285
BcgI	CGANNNNNNTGC	4 cut(s)	301, 335, 1472, 1506
BciT130I	CCWGG	4 cut(s)	163, 603, 666, 708
BciVI	GTATCC	1 cut(s)	1292
BclI	TGATCA	1 cut(s)	1216
BcnI	CCSGG	1 cut(s)	1082
BcoDI	GTCTC	6 cut(s)	298, 447, 663, 1124, 1465, 1826
BfaI	CTAG	1 cut(s)	1148
BfmI	CTRYAG	2 cut(s)	480, 1074
BfoI	RGCGCY	1 cut(s)	288
BfuAI	ACCTGC	2 cut(s)	870, 1988
BfuI	GTATCC	1 cut(s)	1292
BglII	AGATCT	2 cut(s)	40, 316
Bme1390I	CCNGG	5 cut(s)	163, 603, 666, 708, 1082
BmeT110I	CYCGRG	2 cut(s)	238, 441
BmiI	GGNNCC	4 cut(s)	160, 245, 340, 1440
BmrFI	CCNGG	5 cut(s)	163, 603, 666, 708, 1082
BmsI	GCATC	2 cut(s)	1298, 1492
BplI	GAGNNNNNCTC	2 cut(s)	306, 338
BpmI	CTGGAG	2 cut(s)	1524, 1794
BpuEI	CTTGAG	1 cut(s)	2236
BpuMI	CCSGG	1 cut(s)	1082
BsaBI	GATNNNNATC	1 cut(s)	1221
BsaHI	GRCGYC	1 cut(s)	6
BsaI	GGTCTC	1 cut(s)	663
BsaJI	CCNNGG	8 cut(s)	239, 342, 355, 614, 1059, 1081, 2022, 2085
BsaXI	ACNNNNNCTCC	4 cut(s)	660, 690, 2226, 2256
Bsc4I	CCNNNNNNNGG	4 cut(s)	425, 1337, 2023, 2278
Bse118I	RCCGGY	1 cut(s)	33
Bse1I	ACTGG	4 cut(s)	430, 1507, 1755, 1777
Bse3DI	GCAATG	3 cut(s)	1050, 1521, 2053
Bse8I	GATNNNNATC	1 cut(s)	1221
BseBI	CCWGG	4 cut(s)	163, 603, 666, 708
BseDI	CCNNGG	8 cut(s)	239, 342, 355, 614, 1059, 1081, 2022, 2085
BseGI	GGATG	5 cut(s)	461, 631, 646, 1289, 2095
BseJI	GATNNNNATC	1 cut(s)	1221
BseLI	CCNNNNNNNGG	4 cut(s)	425, 1337, 2023, 2278
BseMI	GCAATG	3 cut(s)	1050, 1521, 2053
BseMII	CTCAG	5 cut(s)	161, 522, 579, 1476, 1759
BseNI	ACTGG	4 cut(s)	430, 1507, 1755, 1777
BsePI	GCGCGC	1 cut(s)	2185
BseRI	GAGGAG	1 cut(s)	1952
BseXI	GCAGC	7 cut(s)	759, 1096, 1264, 1306, 1309, 1870, 2101
BsgI	GTGCAG	1 cut(s)	1327
Bsh1236I	CGCG	6 cut(s)	209, 298, 616, 2024, 2187, 2189
BshFI	GGCC	6 cut(s)	89, 212, 388, 1080, 1712, 2210
BshNI	GGYRCC	1 cut(s)	243
BsiHKAI	GWGCWC	1 cut(s)	1532
BsiHKCI	CYCGRG	2 cut(s)	238, 441
BsiSI	CCGG	2 cut(s)	34, 1081
BslI	CCNNNNNNNGG	4 cut(s)	425, 1337, 2023, 2278
BsmAI	GTCTC	6 cut(s)	298, 447, 663, 1124, 1465, 1826
BsmBI	CGTCTC	1 cut(s)	1826
BsmI	GAATGC	2 cut(s)	1599, 2224
BsnI	GGCC	6 cut(s)	89, 212, 388, 1080, 1712, 2210
Bso31I	GGTCTC	1 cut(s)	663
BsoBI	CYCGRG	2 cut(s)	238, 441
Bsp1286I	GDGCHC	1 cut(s)	1532
Bsp19I	CCATGG	2 cut(s)	342, 2085
BspANI	GGCC	6 cut(s)	89, 212, 388, 1080, 1712, 2210
BspCNI	CTCAG	5 cut(s)	162, 523, 580, 1475, 1758
BspFNI	CGCG	6 cut(s)	209, 298, 616, 2024, 2187, 2189
BspLI	GGNNCC	4 cut(s)	160, 245, 340, 1440
BspMI	ACCTGC	2 cut(s)	870, 1988
BspPI	GGATC	7 cut(s)	239, 493, 1001, 1131, 1433, 1437, 1446
BspQI	GCTCTTC	1 cut(s)	708
BspT107I	GGYRCC	1 cut(s)	243
BspTNI	GGTCTC	1 cut(s)	663
BsrBI	CCGCTC	1 cut(s)	1942
BsrDI	GCAATG	3 cut(s)	1050, 1521, 2053
BsrFI	RCCGGY	1 cut(s)	33
BsrI	ACTGG	4 cut(s)	430, 1507, 1755, 1777
BssAI	RCCGGY	1 cut(s)	33
BssECI	CCNNGG	8 cut(s)	239, 342, 355, 614, 1059, 1081, 2022, 2085
BssHII	GCGCGC	1 cut(s)	2185
BssNI	GRCGYC	1 cut(s)	6
BssT1I	CCWWGG	4 cut(s)	342, 355, 1059, 2085
Bst2UI	CCWGG	4 cut(s)	163, 603, 666, 708
Bst6I	CTCTTC	3 cut(s)	708, 1537, 2134
BstACI	GRCGYC	1 cut(s)	6
BstAPI	GCANNNNNTGC	1 cut(s)	1474
BstC8I	GCNNGC	2 cut(s)	1481, 2187
BstDEI	CTNAG	7 cut(s)	170, 531, 588, 1034, 1462, 1745, 2212
BstDSI	CCRYGG	4 cut(s)	342, 614, 2022, 2085
BstENI	CCTNNNNNAGG	1 cut(s)	2276
BstF5I	GGATG	5 cut(s)	461, 631, 646, 1289, 2095
BstFNI	CGCG	6 cut(s)	209, 298, 616, 2024, 2187, 2189
BstH2I	RGCGCY	1 cut(s)	288
BstHHI	GCGC	5 cut(s)	287, 300, 1479, 2187, 2189
BstMAI	GTCTC	6 cut(s)	298, 447, 663, 1124, 1465, 1826
BstMWI	GCNNNNNNNGC	6 cut(s)	357, 723, 1061, 1474, 1636, 2193
BstNI	CCWGG	4 cut(s)	163, 603, 666, 708
BstNSI	RCATGY	2 cut(s)	1472, 1483
BstSCI	CCNGG	5 cut(s)	161, 601, 664, 706, 1080
BstSFI	CTRYAG	2 cut(s)	480, 1074
BstUI	CGCG	6 cut(s)	209, 298, 616, 2024, 2187, 2189
BstV1I	GCAGC	7 cut(s)	759, 1096, 1264, 1306, 1309, 1870, 2101
BstX2I	RGATCY	3 cut(s)	40, 316, 1438
BstYI	RGATCY	3 cut(s)	40, 316, 1438
BsuI	GTATCC	1 cut(s)	1292
BsuRI	GGCC	6 cut(s)	89, 212, 388, 1080, 1712, 2210
BtgI	CCRYGG	4 cut(s)	342, 614, 2022, 2085
BtgZI	GCGATG	1 cut(s)	1175
BtsCI	GGATG	5 cut(s)	461, 631, 646, 1289, 2095
BtsI	GCAGTG	1 cut(s)	1315
BtsIMutI	CAGTG	5 cut(s)	213, 627, 1315, 2103, 2328
BveI	ACCTGC	2 cut(s)	870, 1988
Cac8I	GCNNGC	2 cut(s)	1481, 2187
CaiI	CAGNNNCTG	1 cut(s)	1112
CfoI	GCGC	5 cut(s)	287, 300, 1479, 2187, 2189
Cfr10I	RCCGGY	1 cut(s)	33
Cfr42I	CCGCGG	2 cut(s)	617, 2025
CseI	GACGC	3 cut(s)	215, 340, 1889
Csp6I	GTAC	7 cut(s)	244, 331, 535, 966, 2052, 2171, 2180
CviQI	GTAC	7 cut(s)	244, 331, 535, 966, 2052, 2171, 2180
DdeI	CTNAG	7 cut(s)	170, 531, 588, 1034, 1462, 1745, 2212
DraI	TTTAAA	2 cut(s)	1899, 2163
DrdI	GACNNNNNNGTC	1 cut(s)	1457
DseDI	GACNNNNNNGTC	1 cut(s)	1457
EaeI	YGGCCR	1 cut(s)	210
Eam1104I	CTCTTC	3 cut(s)	708, 1537, 2134
EarI	CTCTTC	3 cut(s)	708, 1537, 2134
Ecl136II	GAGCTC	1 cut(s)	1530
Eco130I	CCWWGG	4 cut(s)	342, 355, 1059, 2085
Eco147I	AGGCCT	2 cut(s)	1712, 2210
Eco24I	GRGCYC	1 cut(s)	1532
Eco31I	GGTCTC	1 cut(s)	663
Eco53kI	GAGCTC	1 cut(s)	1530
Eco57I	CTGAAG	1 cut(s)	269
Eco88I	CYCGRG	2 cut(s)	238, 441
EcoICRI	GAGCTC	1 cut(s)	1530
EcoNI	CCTNNNNNAGG	1 cut(s)	2276
EcoRI	GAATTC	1 cut(s)	871
EcoRII	CCWGG	4 cut(s)	161, 601, 664, 706
EcoT14I	CCWWGG	4 cut(s)	342, 355, 1059, 2085
EcoT22I	ATGCAT	2 cut(s)	1291, 1485
EcoT38I	GRGCYC	1 cut(s)	1532
ErhI	CCWWGG	4 cut(s)	342, 355, 1059, 2085
Esp3I	CGTCTC	1 cut(s)	1826
FalI	AAGNNNNNCTT	2 cut(s)	2230, 2262
FauI	CCCGC	2 cut(s)	1935, 2017
FauNDI	CATATG	1 cut(s)	1735
FbaI	TGATCA	1 cut(s)	1216
FblI	GTMKAC	1 cut(s)	123
FokI	GGATG	5 cut(s)	448, 638, 653, 1276, 2102
FriOI	GRGCYC	1 cut(s)	1532
FspBI	CTAG	1 cut(s)	1148
FspI	TGCGCA	1 cut(s)	1478
GlaI	GCGC	5 cut(s)	286, 299, 1478, 2186, 2188
GsuI	CTGGAG	2 cut(s)	1524, 1794
HaeII	RGCGCY	1 cut(s)	288
HaeIII	GGCC	6 cut(s)	89, 212, 388, 1080, 1712, 2210
HapII	CCGG	2 cut(s)	34, 1081
HgaI	GACGC	3 cut(s)	215, 340, 1889
HhaI	GCGC	5 cut(s)	287, 300, 1479, 2187, 2189
Hin1I	GRCGYC	1 cut(s)	6
Hin6I	GCGC	5 cut(s)	285, 298, 1477, 2185, 2187
HinP1I	GCGC	5 cut(s)	285, 298, 1477, 2185, 2187
HincII	GTYRAC	7 cut(s)	124, 1359, 1879, 1926, 2030, 2095, 2227
HindII	GTYRAC	7 cut(s)	124, 1359, 1879, 1926, 2030, 2095, 2227
HinfI	GANTC	6 cut(s)	166, 653, 1388, 1565, 2069, 2108
HpaII	CCGG	2 cut(s)	34, 1081
HphI	GGTGA	8 cut(s)	23, 161, 358, 427, 538, 992, 1016, 1520
Hpy99I	CGWCG	2 cut(s)	209, 2234
HpyAV	CCTTC	6 cut(s)	208, 275, 1137, 2000, 2278, 2283
HpyCH4IV	ACGT	2 cut(s)	6, 854
HpyF10VI	GCNNNNNNNGC	6 cut(s)	357, 723, 1061, 1474, 1636, 2193
HpyF3I	CTNAG	7 cut(s)	170, 531, 588, 1034, 1462, 1745, 2212
HpySE526I	ACGT	2 cut(s)	6, 854
Hsp92I	GRCGYC	1 cut(s)	6
HspAI	GCGC	5 cut(s)	285, 298, 1477, 2185, 2187
KpnI	GGTACC	1 cut(s)	247
Ksp22I	TGATCA	1 cut(s)	1216
KspI	CCGCGG	2 cut(s)	617, 2025
LguI	GCTCTTC	1 cut(s)	708
LmnI	GCTCC	8 cut(s)	604, 1060, 1249, 1527, 1939, 1945, 2009, 2234
Lsp1109I	GCAGC	7 cut(s)	759, 1096, 1264, 1306, 1309, 1870, 2101
LweI	GCATC	2 cut(s)	1298, 1492
MaeI	CTAG	1 cut(s)	1148
MaeII	ACGT	2 cut(s)	6, 854
MaeIII	GTNAC	8 cut(s)	149, 304, 364, 515, 526, 980, 1114, 1261
MbiI	CCGCTC	1 cut(s)	1942
MboII	GAAGA	7 cut(s)	26, 71, 695, 1080, 1554, 1559, 2121
MfeI	CAATTG	1 cut(s)	876
MflI	RGATCY	3 cut(s)	40, 316, 1438
MhlI	GDGCHC	1 cut(s)	1532
MlyI	GAGTC	2 cut(s)	1382, 1559
MmeI	TCCRAC	2 cut(s)	489, 1604
Mph1103I	ATGCAT	2 cut(s)	1291, 1485
MroXI	GAANNNNTTC	2 cut(s)	264, 1861
MslI	CAYNNNNRTG	4 cut(s)	71, 989, 995, 2303
MspA1I	CMGCKG	3 cut(s)	616, 1109, 2024
MspI	CCGG	2 cut(s)	34, 1081
MspR9I	CCNGG	5 cut(s)	163, 603, 666, 708, 1082
MunI	CAATTG	1 cut(s)	876
Mva1269I	GAATGC	2 cut(s)	1599, 2224
MvaI	CCWGG	4 cut(s)	163, 603, 666, 708
MvnI	CGCG	6 cut(s)	209, 298, 616, 2024, 2187, 2189
MwoI	GCNNNNNNNGC	6 cut(s)	357, 723, 1061, 1474, 1636, 2193
NciI	CCSGG	1 cut(s)	1082
NcoI	CCATGG	2 cut(s)	342, 2085
NdeI	CATATG	1 cut(s)	1735
NlaIV	GGNNCC	4 cut(s)	160, 245, 340, 1440
NmeAIII	GCCGAG	1 cut(s)	1511
NmuCI	GTSAC	5 cut(s)	149, 304, 364, 526, 980
NsbI	TGCGCA	1 cut(s)	1478
NsiI	ATGCAT	2 cut(s)	1291, 1485
NspI	RCATGY	2 cut(s)	1472, 1483
PaeI	GCATGC	1 cut(s)	1483
PaeR7I	CTCGAG	1 cut(s)	441
PauI	GCGCGC	1 cut(s)	2185
PceI	AGGCCT	2 cut(s)	1712, 2210
PciSI	GCTCTTC	1 cut(s)	708
PcsI	WCGNNNNNNNCGW	2 cut(s)	129, 448
PctI	GAATGC	2 cut(s)	1599, 2224
PdmI	GAANNNNTTC	2 cut(s)	264, 1861
PfeI	GAWTC	4 cut(s)	166, 653, 2069, 2108
PfoI	TCCNGGA	1 cut(s)	161
PleI	GAGTC	2 cut(s)	1382, 1559
PpsI	GAGTC	2 cut(s)	1382, 1559
Psp124BI	GAGCTC	1 cut(s)	1532
Psp6I	CCWGG	4 cut(s)	161, 601, 664, 706
PspGI	CCWGG	4 cut(s)	161, 601, 664, 706
PspN4I	GGNNCC	4 cut(s)	160, 245, 340, 1440
PstNI	CAGNNNCTG	1 cut(s)	1112
PsuI	RGATCY	3 cut(s)	40, 316, 1438
PteI	GCGCGC	1 cut(s)	2185
PvuII	CAGCTG	1 cut(s)	1109
RsaI	GTAC	7 cut(s)	245, 332, 536, 967, 2053, 2172, 2181
RsaNI	GTAC	7 cut(s)	244, 331, 535, 966, 2052, 2171, 2180
RseI	CAYNNNNRTG	4 cut(s)	71, 989, 995, 2303
SacI	GAGCTC	1 cut(s)	1532
SacII	CCGCGG	2 cut(s)	617, 2025
SalI	GTCGAC	1 cut(s)	122
SapI	GCTCTTC	1 cut(s)	708
SchI	GAGTC	2 cut(s)	1382, 1559
ScrFI	CCNGG	5 cut(s)	163, 603, 666, 708, 1082
SduI	GDGCHC	1 cut(s)	1532
SfaNI	GCATC	2 cut(s)	1298, 1492
SfcI	CTRYAG	2 cut(s)	480, 1074
Sfr274I	CTCGAG	1 cut(s)	441
Sfr303I	CCGCGG	2 cut(s)	617, 2025
SgrAI	CRCCGGYG	1 cut(s)	33
SgrBI	CCGCGG	2 cut(s)	617, 2025
SlaI	CTCGAG	1 cut(s)	441
SmiMI	CAYNNNNRTG	4 cut(s)	71, 989, 995, 2303
SmlI	CTYRAG	2 cut(s)	441, 2251
SmoI	CTYRAG	2 cut(s)	441, 2251
SphI	GCATGC	1 cut(s)	1483
SseBI	AGGCCT	2 cut(s)	1712, 2210
SspI	AATATT	2 cut(s)	924, 1375
SspMI	CTAG	1 cut(s)	1148
SstI	GAGCTC	1 cut(s)	1532
StuI	AGGCCT	2 cut(s)	1712, 2210
StyD4I	CCNGG	5 cut(s)	161, 601, 664, 706, 1080
StyI	CCWWGG	4 cut(s)	342, 355, 1059, 2085
TaiI	ACGT	2 cut(s)	9, 857
TaqI	TCGA	3 cut(s)	123, 234, 442
TatI	WGTACW	1 cut(s)	330
TauI	GCSGC	3 cut(s)	111, 114, 212
TfiI	GAWTC	4 cut(s)	166, 653, 2069, 2108
TscAI	CASTG	5 cut(s)	220, 627, 1315, 2110, 2328
TseFI	GTSAC	5 cut(s)	149, 304, 364, 526, 980
TseI	GCWGC	7 cut(s)	772, 1109, 1252, 1319, 1322, 1883, 2114
Tsp45I	GTSAC	5 cut(s)	149, 304, 364, 526, 980
TspGWI	ACGGA	5 cut(s)	552, 999, 2188, 2191, 2247
TspRI	CASTG	5 cut(s)	220, 627, 1315, 2110, 2328
XagI	CCTNNNNNAGG	1 cut(s)	2276
XapI	RAATTY	3 cut(s)	871, 1199, 1900
XceI	RCATGY	2 cut(s)	1472, 1483
XhoI	CTCGAG	1 cut(s)	441
XmiI	GTMKAC	1 cut(s)	123
XmnI	GAANNNNTTC	2 cut(s)	264, 1861
XspI	CTAG	1 cut(s)	1148
ZraI	GACGTC	1 cut(s)	7
Zsp2I	ATGCAT	2 cut(s)	1291, 1485

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.