Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr10

Orthogroup ID

OG0007430

Physical Location & Seq

Genomic Coordinates Reverse (-)

24530286 .. 24535033

Gene Structure 4748 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom10g22020.9

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2259 bp

ATGGACATCAGCGAAGAAGTTAGGGCGGTTCACCGGCGAGATCTCCTTGCTTTCTTGGAAGATGGCATATACATGGACGAAATCAAGGCCGTCCTCAACCACAACCGCCGCCGCCTCATTGTTGACATCTCCGATCTCCACTCCTACGGCGACATTGGTTCCAGGATTCTGAGAAATCCAAGTGATTATATGCAATCATTCAACGACGCGGCCACAGAAATCGCCAACAGGATCGACCCGAGGTACCTGAAGCAAGGAGAACAACTTCTGGTGGGGTTTGAAGGCGCTTTTGTCTCACGCCGTGTCACGCCGAGAGATCTGCTCTCCGAGTACATTGGTTCCATGGTTTGCGTCCAAGGAATTGTCACCAAATGTTCTCTTGTGAGGCCAAAAGTTGTTAAGAGTGTTCACTTCTGCCCTACTACCGGAAACTTCACCTCTCGAGAATACCGAGACATTACATCCAACATGGGTTTGCCTACAGGATCAGTTTATCCTACAAGGGATGAAAATGGTAACTTGTTGGTGACTGAGTACGGACTGTGCAAATACAAAGACCATCAAACTTTATCTATGCAAGAAGTTCCTGAGAACTCTGCTCCTGGTCAGCTTCCGCGGACGGTGGATGTCATAGTTGAGGATGACCTTGTTGATTCATGCAAACCAGGAGACCGTGTTGCGATTGTAGGGATATACAAAGCTCTTCCTGGCAAAAGCAAGGGAAGCGTAAATGGAGTATTCAGGACTGTTCTCATAGCGAACAATGTTTCGCTGCTCAACAAAGAAGCAAATGCACCAATTTACAGTCCTGATGACATAAAGAATATAAAAAAGATAGCAGAAAGAAGTGATACGTTTGACCTGCTTGGGAATTCAATTGCACCCTCTATTTATGGACATTCATGGATAAAGAAAGCAGTAATATTACTAATGCTTGGTGGAATGGAAAAGAACTTAAAGAATGGTACTCACTTACGAGGTGACATTAACATGATGATGGTTGGTGATCCATCTGTTGCCAAATCTCAACTTCTAAGAGCAATTATGAAGATCGCTCCCTTGGCAATATCAACTACAGGCCGGGGTTCTTCTGGTGTTGGCTTGACAGCGGCTGTTACTTCGGATCAAGAGACAGGGGAGAGAAGGCTAGAAGCTGGTGCGATGGTTCTTGCTGATCGTGGTGTTGTCTGTATTGATGAATTTGACAAAATGAATGATCAAGATCGTGTTGCCATACATGAAGTTATGGAGCAGCAGACTGTAACTATTGCCAAAGCTGGTATCCATGCATCCCTCAATGCACGGTGCAGTGTGGTGGCTGCTGCAAATCCCATATATGGTTCTTATGATCGCTCTTTGACACCAACAAAGAATATTGGACTTCCAGACTCTTTGCTTTCTCGTTTTGATTTACTATTTATCGTATTGGATCAAATGGATCCTGACATTGACCGTCATGTCTCAGAGCATGTGTTGCGCATGCATCGGTATCGTTCTGCAACTGGAGGTGAGGCAATGCTTGATGGGAGCTCGGCATTTGGAAGAGAAGATGAAACTGATAACGACTCATCTGTATTTGTTAAGTATTACCGAATGCTACATGGGAAAAAAACCGATAGGGGTCGGAAGCGTGATACGCTCACAATCAAGTTTCTTAAAAAGTATATTCATTATGCAAAGAACAGGATTGAGCCTGATCTCACTGATGAGGCCTCTGAACAAATTGCAACAGCATATGCAGAACTCAGAAATGCCAGTTCAAATGCAAAGACCGGAGGAACACTTCCAATTACAGCCAGAACTTTAGAAACCATCATTCGTCTCTCAACTGCTCATGCGAAATTAAAGTTGAGCAGAAAGGTTTCAAAGTCTGATGTTGACGCTGCTCTTAAAGTTTTAAATTTTGCAATATATCACAAAGAGTTGACTGACATGGAGGAGCGGGAGCAAGAAAGAGAGAGAGAATTGGAGAGGAACGGCGCAGCTAACCATTCCGCAGGTCAAGAAGGGTTAAAAGCACTTACTGATGTTCTAAATCAAGTACGGACAGTACAGCGCTCGGAAAGCATATCCGTTGAGGCCTTAGAGAATGCTGTCAATGACGGAGCAAGTGTTCCTTACTCAAGGGCAGAGATAACCTTCCTACTAGAGGGAGAGCAGAATTTGGCAACAGATCGGAACCATCACGCTTCGATGGAGGAGAATACTGAATTTACTTCCGGTGTTGACCTCATATGTCTTCTTGTCTGTGGTTCTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000347 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006268 GO:0006725 GO:0006807 GO:0006996 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0016043 GO:0032392 GO:0032508 GO:0032991 GO:0034641 GO:0034645 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0046483 GO:0051276 GO:0071103 GO:0071704 GO:0071840 GO:0090304 GO:1901360 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko03030 ko04110 ko04111 ko04113 map03030 map04110 map04111 map04113

Pfam Domains

Protein Families

MCM MCM_N MCM_OB

Protein Analysis

753

Amino Acids

83.13

Weight (kDa)

6.07

Isoelectric Point (pI)

32.42

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007430)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G46280 AT5G46280 AT5G46280 AT5G46280
fragaria_vesca	FvH4_3g05860
malus_domestica	MD05G1286600.v1.1 MD10G1264100.v1.1
prunus_persica	Prupe.4G078300_v2.0.a1
pyrus_communis	pycom10g22020
rosa_chinensis	RchiOBHm_Chr5g0013601 RchiOBHm_Chr5g0013661
rosa_laevigata	RLG00000032038
rosa_multiflora	Rmu_sc0002573.1_g000006 Rmu_sc0002573.1_g000015
rosa_roxburghii	Rroxscaffold_1G00062940
rosa_rugosa	Rorug05G0011700 Rorug05G0011800
rosa_samantha	Rh5AG106100 Rh5AG106300 Rh5BG102500 Rh5CG114000 Rh5CG114400 Rh5DG100800 Rh5DG101200
rosa_wichuraiana	Rw5G009270

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1457
Acc16I	TGCGCA	1 cut(s)	1478
Acc36I	ACCTGC	2 cut(s)	870, 1988
Acc65I	GGTACC	1 cut(s)	243
AccB1I	GGYRCC	1 cut(s)	243
AccBSI	CCGCTC	1 cut(s)	1942
AccII	CGCG	2 cut(s)	209, 616
AclWI	GGATC	7 cut(s)	239, 493, 1001, 1131, 1433, 1437, 1446
AcoI	YGGCCR	1 cut(s)	210
AcsI	RAATTY	5 cut(s)	871, 1199, 1900, 2161, 2210
AcuI	CTGAAG	1 cut(s)	269
AdeI	CACNNNGTG	1 cut(s)	302
AfaI	GTAC	6 cut(s)	245, 332, 536, 967, 2043, 2052
AfeI	AGCGCT	1 cut(s)	2057
AfiI	CCNNNNNNNGG	3 cut(s)	425, 1337, 2149
AgsI	TTSAA	5 cut(s)	202, 281, 876, 1761, 1866
AjnI	CCWGG	4 cut(s)	161, 601, 664, 706
AloI	GAACNNNNNNTCC	2 cut(s)	2097, 2129
AluBI	AGCT	6 cut(s)	610, 701, 1154, 1277, 1530, 1985
AluI	AGCT	6 cut(s)	610, 701, 1154, 1277, 1530, 1985
Alw21I	GWGCWC	1 cut(s)	1532
Alw26I	GTCTC	6 cut(s)	298, 447, 663, 1124, 1465, 1826
AlwI	GGATC	7 cut(s)	239, 493, 1001, 1131, 1433, 1437, 1446
AlwNI	CAGNNNCTG	1 cut(s)	1112
Ama87I	CYCGRG	2 cut(s)	238, 441
Aor51HI	AGCGCT	1 cut(s)	2057
AoxI	GGCC	6 cut(s)	87, 210, 386, 1078, 1710, 2079
ApeKI	GCWGC	6 cut(s)	772, 1252, 1319, 1322, 1883, 1982
ApoI	RAATTY	5 cut(s)	871, 1199, 1900, 2161, 2210
Asp700I	GAANNNNTTC	2 cut(s)	264, 1861
Asp718I	GGTACC	1 cut(s)	243
AspLEI	GCGC	4 cut(s)	287, 1479, 1982, 2058
AsuC2I	CCSGG	1 cut(s)	1082
AsuHPI	GGTGA	7 cut(s)	23, 358, 427, 538, 992, 1016, 1520
AvaI	CYCGRG	2 cut(s)	238, 441
BamHI	GGATCC	1 cut(s)	1438
BanI	GGYRCC	1 cut(s)	243
BanII	GRGCYC	1 cut(s)	1532
BbsI	GAAGAC	1 cut(s)	2231
Bbv12I	GWGCWC	1 cut(s)	1532
BbvI	GCAGC	6 cut(s)	759, 1264, 1306, 1309, 1870, 1994
BccI	CCATC	9 cut(s)	56, 567, 991, 1018, 1156, 1517, 1820, 2188, 2190
BceAI	ACGGC	4 cut(s)	74, 163, 285, 1993
BcgI	CGANNNNNNTGC	4 cut(s)	301, 335, 1472, 1506
BciT130I	CCWGG	4 cut(s)	163, 603, 666, 708
BciVI	GTATCC	1 cut(s)	1292
BclI	TGATCA	1 cut(s)	1216
BcnI	CCSGG	1 cut(s)	1082
BcoDI	GTCTC	6 cut(s)	298, 447, 663, 1124, 1465, 1826
BfaI	CTAG	2 cut(s)	1148, 2147
BfmI	CTRYAG	2 cut(s)	480, 1074
BfoI	RGCGCY	2 cut(s)	288, 2059
BfuAI	ACCTGC	2 cut(s)	870, 1988
BfuI	GTATCC	1 cut(s)	1292
BglII	AGATCT	2 cut(s)	40, 316
Bme1390I	CCNGG	5 cut(s)	163, 603, 666, 708, 1082
BmeT110I	CYCGRG	2 cut(s)	238, 441
BmiI	GGNNCC	5 cut(s)	160, 245, 340, 1440, 2180
BmrFI	CCNGG	5 cut(s)	163, 603, 666, 708, 1082
BmsI	GCATC	2 cut(s)	1298, 1492
BpiI	GAAGAC	1 cut(s)	2231
BplI	GAGNNNNNCTC	2 cut(s)	306, 338
BpmI	CTGGAG	1 cut(s)	1524
BpuEI	CTTGAG	1 cut(s)	2107
BpuMI	CCSGG	1 cut(s)	1082
BsaBI	GATNNNNATC	1 cut(s)	1221
BsaI	GGTCTC	1 cut(s)	663
BsaJI	CCNNGG	6 cut(s)	239, 342, 355, 614, 1059, 1081
BsaWI	WCCGGW	3 cut(s)	425, 1772, 2219
BsaXI	ACNNNNNCTCC	4 cut(s)	660, 690, 2097, 2127
Bsc4I	CCNNNNNNNGG	3 cut(s)	425, 1337, 2149
Bse118I	RCCGGY	1 cut(s)	33
Bse1I	ACTGG	2 cut(s)	1507, 1755
Bse3DI	GCAATG	1 cut(s)	1521
Bse8I	GATNNNNATC	1 cut(s)	1221
BseBI	CCWGG	4 cut(s)	163, 603, 666, 708
BseDI	CCNNGG	6 cut(s)	239, 342, 355, 614, 1059, 1081
BseGI	GGATG	5 cut(s)	461, 511, 631, 646, 1289
BseJI	GATNNNNATC	1 cut(s)	1221
BseLI	CCNNNNNNNGG	3 cut(s)	425, 1337, 2149
BseMI	GCAATG	1 cut(s)	1521
BseMII	CTCAG	5 cut(s)	161, 522, 579, 1476, 1759
BseNI	ACTGG	2 cut(s)	1507, 1755
BseRI	GAGGAG	2 cut(s)	1952, 2213
BseXI	GCAGC	6 cut(s)	759, 1264, 1306, 1309, 1870, 1994
BsgI	GTGCAG	1 cut(s)	1327
Bsh1236I	CGCG	2 cut(s)	209, 616
BshFI	GGCC	6 cut(s)	89, 212, 388, 1080, 1712, 2081
BshNI	GGYRCC	1 cut(s)	243
BsiHKAI	GWGCWC	1 cut(s)	1532
BsiHKCI	CYCGRG	2 cut(s)	238, 441
BsiSI	CCGG	5 cut(s)	34, 426, 1081, 1773, 2220
BslI	CCNNNNNNNGG	3 cut(s)	425, 1337, 2149
BsmAI	GTCTC	6 cut(s)	298, 447, 663, 1124, 1465, 1826
BsmBI	CGTCTC	1 cut(s)	1826
BsmI	GAATGC	2 cut(s)	1599, 2095
BsnI	GGCC	6 cut(s)	89, 212, 388, 1080, 1712, 2081
Bso31I	GGTCTC	1 cut(s)	663
BsoBI	CYCGRG	2 cut(s)	238, 441
Bsp1286I	GDGCHC	1 cut(s)	1532
Bsp19I	CCATGG	1 cut(s)	342
BspANI	GGCC	6 cut(s)	89, 212, 388, 1080, 1712, 2081
BspCNI	CTCAG	5 cut(s)	162, 523, 580, 1475, 1758
BspFNI	CGCG	2 cut(s)	209, 616
BspLI	GGNNCC	5 cut(s)	160, 245, 340, 1440, 2180
BspMI	ACCTGC	2 cut(s)	870, 1988
BspPI	GGATC	7 cut(s)	239, 493, 1001, 1131, 1433, 1437, 1446
BspQI	GCTCTTC	1 cut(s)	708
BspT107I	GGYRCC	1 cut(s)	243
BspTNI	GGTCTC	1 cut(s)	663
BsrBI	CCGCTC	1 cut(s)	1942
BsrDI	GCAATG	1 cut(s)	1521
BsrFI	RCCGGY	1 cut(s)	33
BsrI	ACTGG	2 cut(s)	1507, 1755
BssAI	RCCGGY	1 cut(s)	33
BssECI	CCNNGG	6 cut(s)	239, 342, 355, 614, 1059, 1081
BssT1I	CCWWGG	3 cut(s)	342, 355, 1059
Bst2UI	CCWGG	4 cut(s)	163, 603, 666, 708
Bst4CI	ACNGT	9 cut(s)	543, 622, 674, 748, 806, 1261, 1305, 1454, 2050
Bst6I	CTCTTC	2 cut(s)	708, 1537
BstAPI	GCANNNNNTGC	1 cut(s)	1474
BstC8I	GCNNGC	1 cut(s)	1481
BstDEI	CTNAG	8 cut(s)	170, 531, 588, 1034, 1462, 1745, 2083, 2256
BstDSI	CCRYGG	2 cut(s)	342, 614
BstENI	CCTNNNNNAGG	1 cut(s)	2147
BstF5I	GGATG	5 cut(s)	461, 511, 631, 646, 1289
BstFNI	CGCG	2 cut(s)	209, 616
BstH2I	RGCGCY	2 cut(s)	288, 2059
BstHHI	GCGC	4 cut(s)	287, 1479, 1982, 2058
BstMAI	GTCTC	6 cut(s)	298, 447, 663, 1124, 1465, 1826
BstMWI	GCNNNNNNNGC	5 cut(s)	723, 1061, 1474, 1636, 2064
BstNI	CCWGG	4 cut(s)	163, 603, 666, 708
BstNSI	RCATGY	2 cut(s)	1472, 1483
BstSCI	CCNGG	5 cut(s)	161, 601, 664, 706, 1080
BstSFI	CTRYAG	2 cut(s)	480, 1074
BstUI	CGCG	2 cut(s)	209, 616
BstV1I	GCAGC	6 cut(s)	759, 1264, 1306, 1309, 1870, 1994
BstV2I	GAAGAC	1 cut(s)	2231
BstX2I	RGATCY	3 cut(s)	40, 316, 1438
BstYI	RGATCY	3 cut(s)	40, 316, 1438
BsuI	GTATCC	1 cut(s)	1292
BsuRI	GGCC	6 cut(s)	89, 212, 388, 1080, 1712, 2081
BtgI	CCRYGG	2 cut(s)	342, 614
BtgZI	GCGATG	1 cut(s)	1175
BtsCI	GGATG	5 cut(s)	461, 511, 631, 646, 1289
BtsI	GCAGTG	1 cut(s)	1315
BtsIMutI	CAGTG	2 cut(s)	1315, 1701
BveI	ACCTGC	2 cut(s)	870, 1988
Cac8I	GCNNGC	1 cut(s)	1481
CaiI	CAGNNNCTG	1 cut(s)	1112
CfoI	GCGC	4 cut(s)	287, 1479, 1982, 2058
Cfr10I	RCCGGY	1 cut(s)	33
Cfr42I	CCGCGG	1 cut(s)	617
CseI	GACGC	3 cut(s)	215, 340, 1889
Csp6I	GTAC	6 cut(s)	244, 331, 535, 966, 2042, 2051
CviQI	GTAC	6 cut(s)	244, 331, 535, 966, 2042, 2051
DdeI	CTNAG	8 cut(s)	170, 531, 588, 1034, 1462, 1745, 2083, 2256
DraI	TTTAAA	1 cut(s)	1899
DraIII	CACNNNGTG	1 cut(s)	302
DrdI	GACNNNNNNGTC	1 cut(s)	1457
DseDI	GACNNNNNNGTC	1 cut(s)	1457
EaeI	YGGCCR	1 cut(s)	210
Eam1104I	CTCTTC	2 cut(s)	708, 1537
EarI	CTCTTC	2 cut(s)	708, 1537
Ecl136II	GAGCTC	1 cut(s)	1530
Eco130I	CCWWGG	3 cut(s)	342, 355, 1059
Eco147I	AGGCCT	2 cut(s)	1712, 2081
Eco24I	GRGCYC	1 cut(s)	1532
Eco31I	GGTCTC	1 cut(s)	663
Eco47III	AGCGCT	1 cut(s)	2057
Eco53kI	GAGCTC	1 cut(s)	1530
Eco57I	CTGAAG	1 cut(s)	269
Eco88I	CYCGRG	2 cut(s)	238, 441
EcoICRI	GAGCTC	1 cut(s)	1530
EcoNI	CCTNNNNNAGG	1 cut(s)	2147
EcoRI	GAATTC	1 cut(s)	871
EcoRII	CCWGG	4 cut(s)	161, 601, 664, 706
EcoT14I	CCWWGG	3 cut(s)	342, 355, 1059
EcoT22I	ATGCAT	2 cut(s)	1291, 1485
EcoT38I	GRGCYC	1 cut(s)	1532
ErhI	CCWWGG	3 cut(s)	342, 355, 1059
Esp3I	CGTCTC	1 cut(s)	1826
FalI	AAGNNNNNCTT	2 cut(s)	2101, 2133
FauI	CCCGC	1 cut(s)	1935
FauNDI	CATATG	2 cut(s)	1735, 2234
FbaI	TGATCA	1 cut(s)	1216
FokI	GGATG	5 cut(s)	448, 518, 638, 653, 1276
FriOI	GRGCYC	1 cut(s)	1532
FspBI	CTAG	2 cut(s)	1148, 2147
FspI	TGCGCA	1 cut(s)	1478
GlaI	GCGC	4 cut(s)	286, 1478, 1981, 2057
GsuI	CTGGAG	1 cut(s)	1524
HaeII	RGCGCY	2 cut(s)	288, 2059
HaeIII	GGCC	6 cut(s)	89, 212, 388, 1080, 1712, 2081
HapII	CCGG	5 cut(s)	34, 426, 1081, 1773, 2220
HgaI	GACGC	3 cut(s)	215, 340, 1889
HhaI	GCGC	4 cut(s)	287, 1479, 1982, 2058
Hin6I	GCGC	4 cut(s)	285, 1477, 1980, 2056
HinP1I	GCGC	4 cut(s)	285, 1477, 1980, 2056
HincII	GTYRAC	4 cut(s)	124, 1879, 1926, 2227
HindII	GTYRAC	4 cut(s)	124, 1879, 1926, 2227
HinfI	GANTC	4 cut(s)	166, 653, 1388, 1565
HpaII	CCGG	5 cut(s)	34, 426, 1081, 1773, 2220
HphI	GGTGA	7 cut(s)	23, 358, 427, 538, 992, 1016, 1520
Hpy166II	GTNNAC	6 cut(s)	31, 124, 409, 1879, 1926, 2227
Hpy8I	GTNNAC	6 cut(s)	31, 124, 409, 1879, 1926, 2227
Hpy99I	CGWCG	1 cut(s)	209
HpyAV	CCTTC	4 cut(s)	275, 1137, 2000, 2149
HpyCH4III	ACNGT	9 cut(s)	543, 622, 674, 748, 806, 1261, 1305, 1454, 2050
HpyCH4IV	ACGT	1 cut(s)	854
HpyF10VI	GCNNNNNNNGC	5 cut(s)	723, 1061, 1474, 1636, 2064
HpyF3I	CTNAG	8 cut(s)	170, 531, 588, 1034, 1462, 1745, 2083, 2256
HpySE526I	ACGT	1 cut(s)	854
HspAI	GCGC	4 cut(s)	285, 1477, 1980, 2056
KpnI	GGTACC	1 cut(s)	247
Ksp22I	TGATCA	1 cut(s)	1216
KspI	CCGCGG	1 cut(s)	617
LguI	GCTCTTC	1 cut(s)	708
LmnI	GCTCC	7 cut(s)	604, 1060, 1249, 1527, 1939, 1945, 2105
Lsp1109I	GCAGC	6 cut(s)	759, 1264, 1306, 1309, 1870, 1994
LweI	GCATC	2 cut(s)	1298, 1492
MaeI	CTAG	2 cut(s)	1148, 2147
MaeII	ACGT	1 cut(s)	854
MaeIII	GTNAC	7 cut(s)	304, 364, 515, 526, 980, 1114, 1261
MbiI	CCGCTC	1 cut(s)	1942
MboII	GAAGA	8 cut(s)	26, 71, 695, 1060, 1080, 1554, 1559, 2231
MfeI	CAATTG	1 cut(s)	876
MflI	RGATCY	3 cut(s)	40, 316, 1438
MhlI	GDGCHC	1 cut(s)	1532
MlyI	GAGTC	2 cut(s)	1382, 1559
MmeI	TCCRAC	2 cut(s)	489, 1604
Mph1103I	ATGCAT	2 cut(s)	1291, 1485
MroXI	GAANNNNTTC	2 cut(s)	264, 1861
MseI	TTAA	9 cut(s)	399, 956, 987, 1581, 1656, 1844, 1890, 1898, 2012
MslI	CAYNNNNRTG	3 cut(s)	71, 989, 995
MspA1I	CMGCKG	2 cut(s)	616, 1109
MspI	CCGG	5 cut(s)	34, 426, 1081, 1773, 2220
MspR9I	CCNGG	5 cut(s)	163, 603, 666, 708, 1082
MunI	CAATTG	1 cut(s)	876
Mva1269I	GAATGC	2 cut(s)	1599, 2095
MvaI	CCWGG	4 cut(s)	163, 603, 666, 708
MvnI	CGCG	2 cut(s)	209, 616
MwoI	GCNNNNNNNGC	5 cut(s)	723, 1061, 1474, 1636, 2064
NciI	CCSGG	1 cut(s)	1082
NcoI	CCATGG	1 cut(s)	342
NdeI	CATATG	2 cut(s)	1735, 2234
NlaIV	GGNNCC	5 cut(s)	160, 245, 340, 1440, 2180
NmeAIII	GCCGAG	2 cut(s)	336, 1511
NmuCI	GTSAC	4 cut(s)	304, 364, 526, 980
NsbI	TGCGCA	1 cut(s)	1478
NsiI	ATGCAT	2 cut(s)	1291, 1485
NspI	RCATGY	2 cut(s)	1472, 1483
PaeI	GCATGC	1 cut(s)	1483
PaeR7I	CTCGAG	1 cut(s)	441
PceI	AGGCCT	2 cut(s)	1712, 2081
PciSI	GCTCTTC	1 cut(s)	708
PcsI	WCGNNNNNNNCGW	1 cut(s)	448
PctI	GAATGC	2 cut(s)	1599, 2095
PdmI	GAANNNNTTC	2 cut(s)	264, 1861
PfeI	GAWTC	2 cut(s)	166, 653
PfoI	TCCNGGA	1 cut(s)	161
PleI	GAGTC	2 cut(s)	1382, 1559
PpsI	GAGTC	2 cut(s)	1382, 1559
Psp124BI	GAGCTC	1 cut(s)	1532
Psp6I	CCWGG	4 cut(s)	161, 601, 664, 706
PspGI	CCWGG	4 cut(s)	161, 601, 664, 706
PspN4I	GGNNCC	5 cut(s)	160, 245, 340, 1440, 2180
PstNI	CAGNNNCTG	1 cut(s)	1112
PsuI	RGATCY	3 cut(s)	40, 316, 1438
RsaI	GTAC	6 cut(s)	245, 332, 536, 967, 2043, 2052
RsaNI	GTAC	6 cut(s)	244, 331, 535, 966, 2042, 2051
RseI	CAYNNNNRTG	3 cut(s)	71, 989, 995
SacI	GAGCTC	1 cut(s)	1532
SacII	CCGCGG	1 cut(s)	617
SapI	GCTCTTC	1 cut(s)	708
SaqAI	TTAA	9 cut(s)	399, 956, 987, 1581, 1656, 1844, 1890, 1898, 2012
SchI	GAGTC	2 cut(s)	1382, 1559
ScrFI	CCNGG	5 cut(s)	163, 603, 666, 708, 1082
SduI	GDGCHC	1 cut(s)	1532
SfaNI	GCATC	2 cut(s)	1298, 1492
SfcI	CTRYAG	2 cut(s)	480, 1074
Sfr274I	CTCGAG	1 cut(s)	441
Sfr303I	CCGCGG	1 cut(s)	617
SgrAI	CRCCGGYG	1 cut(s)	33
SgrBI	CCGCGG	1 cut(s)	617
SlaI	CTCGAG	1 cut(s)	441
SmiMI	CAYNNNNRTG	3 cut(s)	71, 989, 995
SmlI	CTYRAG	2 cut(s)	441, 2122
SmoI	CTYRAG	2 cut(s)	441, 2122
SphI	GCATGC	1 cut(s)	1483
SseBI	AGGCCT	2 cut(s)	1712, 2081
SspI	AATATT	2 cut(s)	924, 1375
SspMI	CTAG	2 cut(s)	1148, 2147
SstI	GAGCTC	1 cut(s)	1532
StuI	AGGCCT	2 cut(s)	1712, 2081
StyD4I	CCNGG	5 cut(s)	161, 601, 664, 706, 1080
StyI	CCWWGG	3 cut(s)	342, 355, 1059
TaaI	ACNGT	9 cut(s)	543, 622, 674, 748, 806, 1261, 1305, 1454, 2050
TaiI	ACGT	1 cut(s)	857
TaqI	TCGA	3 cut(s)	234, 442, 2192
TatI	WGTACW	2 cut(s)	330, 2050
TauI	GCSGC	4 cut(s)	111, 114, 212, 1112
TfiI	GAWTC	2 cut(s)	166, 653
Tru1I	TTAA	9 cut(s)	399, 956, 987, 1581, 1656, 1844, 1890, 1898, 2012
Tru9I	TTAA	9 cut(s)	399, 956, 987, 1581, 1656, 1844, 1890, 1898, 2012
TscAI	CASTG	2 cut(s)	1315, 1708
TseFI	GTSAC	4 cut(s)	304, 364, 526, 980
TseI	GCWGC	6 cut(s)	772, 1252, 1319, 1322, 1883, 1982
Tsp45I	GTSAC	4 cut(s)	304, 364, 526, 980
TspDTI	ATGAA	9 cut(s)	522, 645, 891, 1061, 1212, 1226, 1254, 1566, 1658
TspGWI	ACGGA	4 cut(s)	552, 2059, 2062, 2118
TspRI	CASTG	2 cut(s)	1315, 1708
XagI	CCTNNNNNAGG	1 cut(s)	2147
XapI	RAATTY	5 cut(s)	871, 1199, 1900, 2161, 2210
XceI	RCATGY	2 cut(s)	1472, 1483
XhoI	CTCGAG	1 cut(s)	441
XmnI	GAANNNNTTC	2 cut(s)	264, 1861
XspI	CTAG	2 cut(s)	1148, 2147
Zsp2I	ATGCAT	2 cut(s)	1291, 1485

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.