Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr5A

Orthogroup ID

OG0007430

Physical Location & Seq

Genomic Coordinates Forward (+)

9742853 .. 9747090

Gene Structure 4238 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh5AG106300.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2307 bp

ATGGACATCAGCGAAGAAGTCAGGGCCGCTCACCGCCGAGAGCTCACTACCTTCTTGGAAGATGGAACGTACATGGACGACATCAAGGCCGTCCTCAACCGCAAGGACCGCCGCCTCATCGTCAACATCTCCGATCTCCACTCCTACGGCGACATCGGCAACAGGATTCTGAGAAATCCGAGTGATTATATGCAAGCGTTTAACGATGCGGCCACGGAGGTCGCCAGCAAGATCGATCCTAAGTACTTGAAGTCAGGGGATCAGCTTCTTGTGGGGTTTAATGGCCCCTTTGTATCTCGCGGCGTCACGCCGAGAGATCTGCTCTCTGAGTACATTGGATCCATGGTTTCGGTTCGAGGCATTGTCACCAAATGTTCTCTTGTGAGGCCAAAAGTTGTTAAGAGTGTTCATTTCTGCCCTACAACCGGAAACTTTATGACTCGTGAATACCGAGACATTACATCCAACATGGGTTTGCCTACAGGAACAGTTTATCCCACCAGGGATGAAAATGGCAACTTATTGGTGACTGAGTATGGATTGTGCAAATACAAAGATCATCAGACTTTGTCAATGCAAGAAGTGCCTGAAAACTCTGCTCCTGGTCAGCTCCCAAGAACAGTGGATGTCATAGTTGAGGATGACCTTGTAGATAAGTGCAAGCCAGGAGATCGTGTGGCTGTTGTAGGGATTTACAAAGCTCTTCCTGGTAAAAGTAAGGGAAGTGTGAACGGAGTATTCAGGACGGTTCTCATAGCTAACCATGTTACTCAGCTCAACAAAGAGGCACAATCGCCACAATACAGTGCTGAAGACCTGACTAATATAAAAAAGATAGCAGAGAGAGATGATGCATTTGATCTGCTTGGCAATTCACTTGCACCTTCTATTTATGGGCATTCGTGGATAAAGAAGGCAGTGATACTATTAATGCTTGGTGGAGTAGAAAAGAACCTAAAGAATGGCACCCACTTACGAGGTGACATTAACATGATGATGGTTGGTGATCCTTCTGTTGCCAAGTCTCAGCTTTTAAGAGCAATTATGAATATCGCTCCATTGGCAATATCAACTACTGGCCGGGGTTCTTCTGGTGTTGGTTTGACAGCTGCTGTTACTTCAGATCAAGAGACAGGAGAGAGAAGGCTAGAAGCTGGTGCCATGGTTCTTGCTGATCGCGGTGTTGTCTGTATTGATGAATTTGACAAAATGAATGATCAAGATCGCGTTGCCATACATGAAGTTATGGAACAGCAGACTGTTACTATTGCCAAAGCTGGAATCCATGCATCTCTCAACGCGCGGTGCAGTGTGGTGGCTGCTGCAAATCCCATATATGGCTCTTATGATCGTTCATTGACACCAACAAAGAATATCGGACTTCCAGACTCTTTGCTTTCTCGTTTTGATTTGCTGTTTATTGTATTGGATCAAATGGATCCTGATATTGACCGTCAAGTCTCAGAGCATGTATTGCGTATGCACCGGTATCGTTCTGCATCTGGAGGTGAAGCAATGCTTGATGCGAACTCAGGATATGGAGGAGAAGATGAAATTGATAACGACTCCACTGTATTTGTCAAGTATAACCGGATGCTTCATGGAAAGAAAACATCTAGGGGTCAGAAGCGCGATACCCTCACAATCAAGTTTCTAAAGAAGTACATCCATTATGCAAAGCATAGGATTCAACCTGATCTGACTGATGAGGCATCTGAACAAATTGCAACAGCATATGCAGAACTCAGAGATGCCAGTTCAAATGCAAAGACTGGAGGAACACTTCCAATTACTGCCAGAACCTTGGAAACAATCATTCGTCTCTCAACAGCTCATGCAAAATTGAAGTTGAGCAGAAAGGTTACAAAATCTGATGTTGACGCTGCTCTTAAAGTTTTAAACTTCGCAATATATCACAAGGAGTTGACTGACATGGAGGAACGGGAGCAAGAAAGAGAAATGGAGAGGAATGGCAGGGATGTTCATCGTTCAGGTGAAAATGATGGGTCTCGCAATAGAACTGCTAATAGAGATGGTGCAACAATTGATGCGATGGAAATAGATGACCCTCCTACCCAATCTGCTAGTAATATTTCTTCTGATAGAAAAGAAGCATTTAATTCTTTATTTGGGGGGCATATGCGAGAAAAGCGCTTGGATAGCATATCCATTGAGGATATTGAGATGGCTGTCAATGAAAGAGCAGATTCCCCGTACTCAAGGGCAGAGATAATAGTCCTGTTAGAGAAACTGCAAGAGGATAACAGAGTGATGATCGCTGATGGAACAGTGGTTATGATCTCATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000347 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006268 GO:0006725 GO:0006807 GO:0006996 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0016043 GO:0032392 GO:0032508 GO:0032991 GO:0034641 GO:0034645 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0046483 GO:0051276 GO:0071103 GO:0071704 GO:0071840 GO:0090304 GO:1901360 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko03030 ko04110 ko04111 ko04113 map03030 map04110 map04111 map04113

Pfam Domains

Protein Families

MCM MCM_N MCM_OB

Protein Analysis

768

Amino Acids

84.99

Weight (kDa)

6.05

Isoelectric Point (pI)

31.8

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MCM_N	PF14551	16 - 93	9.3e-11	MCM N-terminal domain
MCM_OB	PF17207	105 - 235	5e-31	MCM OB domain
MCM	PF00493	273 - 495	2.9e-96	MCM P-loop domain
Mg_chelatase	PF01078	379 - 446	3.5e-07	Magnesium chelatase, subunit ChlI
MCM_lid	PF17855	551 - 634	2.2e-26	MCM AAA-lid domain
WHD_MCM3_C	PF23191	697 - 767	3.5e-13	MCM3-like, winged helix domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0007430)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G46280 AT5G46280 AT5G46280 AT5G46280
fragaria_vesca	FvH4_3g05860
malus_domestica	MD05G1286600.v1.1 MD10G1264100.v1.1
prunus_persica	Prupe.4G078300_v2.0.a1
pyrus_communis	pycom10g22020
rosa_chinensis	RchiOBHm_Chr5g0013601 RchiOBHm_Chr5g0013661
rosa_laevigata	RLG00000032038
rosa_multiflora	Rmu_sc0002573.1_g000006 Rmu_sc0002573.1_g000015
rosa_roxburghii	Rroxscaffold_1G00062940
rosa_rugosa	Rorug05G0011700 Rorug05G0011800
rosa_samantha	Rh5AG106100 Rh5AG106300 Rh5BG102500 Rh5CG114000 Rh5CG114400 Rh5DG100800 Rh5DG101200
rosa_wichuraiana	Rw5G009270

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1457
AccB1I	GGYRCC	2 cut(s)	965, 1157
AccBSI	CCGCTC	1 cut(s)	29
AccII	CGCG	6 cut(s)	300, 1179, 1227, 1301, 1303, 1632
AciI	CCGC	9 cut(s)	27, 34, 100, 109, 112, 209, 300, 1179, 1303
AclWI	GGATC	8 cut(s)	230, 267, 333, 346, 1001, 1433, 1437, 1446
AcoI	YGGCCR	2 cut(s)	210, 1078
AcsI	RAATTY	1 cut(s)	1199
AcuI	CTGAAG	2 cut(s)	831, 1104
AcyI	GRCGYC	1 cut(s)	303
AfaI	GTAC	5 cut(s)	71, 245, 332, 1664, 2216
AfeI	AGCGCT	1 cut(s)	2153
AfiI	CCNNNNNNNGG	2 cut(s)	425, 1337
AgeI	ACCGGT	1 cut(s)	1485
AgsI	TTSAA	4 cut(s)	250, 1691, 1761, 1846
AjnI	CCWGG	4 cut(s)	500, 601, 664, 706
AloI	GAACNNNNNNTCC	2 cut(s)	478, 510
Alw21I	GWGCWC	1 cut(s)	45
Alw26I	GTCTC	6 cut(s)	447, 1029, 1124, 1465, 1826, 2013
AlwI	GGATC	8 cut(s)	230, 267, 333, 346, 1001, 1433, 1437, 1446
AlwNI	CAGNNNCTG	1 cut(s)	1112
Aor51HI	AGCGCT	1 cut(s)	2153
AoxI	GGCC	6 cut(s)	24, 87, 210, 283, 386, 1078
ApeKI	GCWGC	4 cut(s)	1109, 1319, 1322, 1883
ApoI	RAATTY	1 cut(s)	1199
AseI	ATTAAT	1 cut(s)	929
AsiGI	ACCGGT	1 cut(s)	1485
AspLEI	GCGC	3 cut(s)	1303, 1632, 2154
AspS9I	GGNCC	3 cut(s)	24, 106, 284
AsuC2I	CCSGG	1 cut(s)	1082
AsuHPI	GGTGA	7 cut(s)	23, 358, 538, 992, 1016, 1520, 2006
AvaII	GGWCC	1 cut(s)	106
BamHI	GGATCC	2 cut(s)	338, 1438
BanI	GGYRCC	2 cut(s)	965, 1157
BanII	GRGCYC	1 cut(s)	45
BarI	GAAGNNNNNNTAC	2 cut(s)	2080, 2112
BauI	CACGAG	1 cut(s)	441
BbsI	GAAGAC	1 cut(s)	819
Bbv12I	GWGCWC	1 cut(s)	45
BbvI	GCAGC	4 cut(s)	1096, 1306, 1309, 1870
BccI	CCATC	7 cut(s)	56, 991, 1997, 2027, 2047, 2179, 2276
BceAI	ACGGC	2 cut(s)	74, 163
BcgI	CGANNNNNNTGC	4 cut(s)	301, 335, 1472, 1506
BciT130I	CCWGG	4 cut(s)	502, 603, 666, 708
BclI	TGATCA	1 cut(s)	1216
BcnI	CCSGG	1 cut(s)	1082
BcoDI	GTCTC	6 cut(s)	447, 1029, 1124, 1465, 1826, 2013
BfaI	CTAG	3 cut(s)	1148, 1617, 2085
BfmI	CTRYAG	1 cut(s)	480
BfoI	RGCGCY	1 cut(s)	2155
BglII	AGATCT	1 cut(s)	316
BisI	GCNGC	8 cut(s)	27, 112, 210, 301, 1110, 1320, 1323, 1884
BlsI	GCNGC	8 cut(s)	28, 113, 211, 302, 1111, 1321, 1324, 1885
BmcAI	AGTACT	1 cut(s)	245
Bme1390I	CCNGG	5 cut(s)	502, 603, 666, 708, 1082
Bme18I	GGWCC	1 cut(s)	106
BmgT120I	GGNCC	3 cut(s)	24, 106, 284
BmiI	GGNNCC	5 cut(s)	286, 340, 967, 1159, 1440
BmrFI	CCNGG	5 cut(s)	502, 603, 666, 708, 1082
BmsI	GCATC	9 cut(s)	196, 841, 1298, 1508, 1513, 1584, 1721, 1741, 2038
BpiI	GAAGAC	1 cut(s)	819
BplI	GAGNNNNNCTC	2 cut(s)	306, 338
BpmI	CTGGAG	2 cut(s)	1524, 1794
BpuEI	CTTGAG	1 cut(s)	2203
BpuMI	CCSGG	1 cut(s)	1082
Bsa29I	ATCGAT	1 cut(s)	234
BsaBI	GATNNNNATC	2 cut(s)	1221, 1983
BsaHI	GRCGYC	1 cut(s)	303
BsaI	GGTCTC	1 cut(s)	2013
BsaJI	CCNNGG	6 cut(s)	213, 342, 501, 1081, 1161, 1803
BsaWI	WCCGGW	3 cut(s)	425, 1485, 1590
BsaXI	ACNNNNNCTCC	2 cut(s)	660, 690
Bsc4I	CCNNNNNNNGG	2 cut(s)	425, 1337
Bse118I	RCCGGY	1 cut(s)	1485
Bse1I	ACTGG	3 cut(s)	1081, 1755, 1777
Bse3DI	GCAATG	1 cut(s)	1521
Bse8I	GATNNNNATC	2 cut(s)	1221, 1983
BseBI	CCWGG	4 cut(s)	502, 603, 666, 708
BseCI	ATCGAT	1 cut(s)	234
BseDI	CCNNGG	6 cut(s)	213, 342, 501, 1081, 1161, 1803
BseGI	GGATG	7 cut(s)	461, 511, 631, 646, 1599, 1665, 1984
BseJI	GATNNNNATC	2 cut(s)	1221, 1983
BseLI	CCNNNNNNNGG	2 cut(s)	425, 1337
BseMI	GCAATG	1 cut(s)	1521
BseMII	CTCAG	8 cut(s)	161, 318, 522, 785, 1040, 1476, 1545, 1759
BseNI	ACTGG	3 cut(s)	1081, 1755, 1777
BseRI	GAGGAG	1 cut(s)	1557
BseXI	GCAGC	4 cut(s)	1096, 1306, 1309, 1870
BsgI	GTGCAG	1 cut(s)	1327
Bsh1236I	CGCG	6 cut(s)	300, 1179, 1227, 1301, 1303, 1632
BshFI	GGCC	6 cut(s)	26, 89, 212, 285, 388, 1080
BshNI	GGYRCC	2 cut(s)	965, 1157
BshTI	ACCGGT	1 cut(s)	1485
BshVI	ATCGAT	1 cut(s)	234
BsiHKAI	GWGCWC	1 cut(s)	45
BsiSI	CCGG	4 cut(s)	426, 1081, 1486, 1591
BslI	CCNNNNNNNGG	2 cut(s)	425, 1337
BsmAI	GTCTC	6 cut(s)	447, 1029, 1124, 1465, 1826, 2013
BsmBI	CGTCTC	1 cut(s)	1826
BsmI	GAATGC	1 cut(s)	898
BsnI	GGCC	6 cut(s)	26, 89, 212, 285, 388, 1080
Bso31I	GGTCTC	1 cut(s)	2013
Bsp1286I	GDGCHC	1 cut(s)	45
Bsp19I	CCATGG	2 cut(s)	342, 1161
BspACI	CCGC	9 cut(s)	27, 34, 100, 109, 112, 209, 300, 1179, 1303
BspANI	GGCC	6 cut(s)	26, 89, 212, 285, 388, 1080
BspCNI	CTCAG	8 cut(s)	162, 319, 523, 784, 1039, 1475, 1544, 1758
BspDI	ATCGAT	1 cut(s)	234
BspFNI	CGCG	6 cut(s)	300, 1179, 1227, 1301, 1303, 1632
BspLI	GGNNCC	5 cut(s)	286, 340, 967, 1159, 1440
BspPI	GGATC	8 cut(s)	230, 267, 333, 346, 1001, 1433, 1437, 1446
BspQI	GCTCTTC	1 cut(s)	708
BspT107I	GGYRCC	2 cut(s)	965, 1157
BspTNI	GGTCTC	1 cut(s)	2013
BsrBI	CCGCTC	1 cut(s)	29
BsrDI	GCAATG	1 cut(s)	1521
BsrFI	RCCGGY	1 cut(s)	1485
BsrI	ACTGG	3 cut(s)	1081, 1755, 1777
BssAI	RCCGGY	1 cut(s)	1485
BssECI	CCNNGG	6 cut(s)	213, 342, 501, 1081, 1161, 1803
BssNI	GRCGYC	1 cut(s)	303
BssSI	CACGAG	1 cut(s)	441
BssT1I	CCWWGG	3 cut(s)	342, 1161, 1803
Bst2BI	CACGAG	1 cut(s)	441
Bst2UI	CCWGG	4 cut(s)	502, 603, 666, 708
Bst4CI	ACNGT	8 cut(s)	490, 622, 748, 806, 1261, 1454, 1573, 2290
Bst6I	CTCTTC	1 cut(s)	708
BstACI	GRCGYC	1 cut(s)	303
BstAPI	GCANNNNNTGC	2 cut(s)	583, 1474
BstC8I	GCNNGC	3 cut(s)	195, 226, 662
BstDEI	CTNAG	9 cut(s)	170, 240, 327, 531, 771, 1026, 1462, 1531, 1745
BstDSI	CCRYGG	3 cut(s)	213, 342, 1161
BstF5I	GGATG	7 cut(s)	461, 511, 631, 646, 1599, 1665, 1984
BstFNI	CGCG	6 cut(s)	300, 1179, 1227, 1301, 1303, 1632
BstH2I	RGCGCY	1 cut(s)	2155
BstHHI	GCGC	3 cut(s)	1303, 1632, 2154
BstMAI	GTCTC	6 cut(s)	447, 1029, 1124, 1465, 1826, 2013
BstMWI	GCNNNNNNNGC	7 cut(s)	108, 156, 583, 1061, 1474, 2149, 2160
BstNI	CCWGG	4 cut(s)	502, 603, 666, 708
BstNSI	RCATGY	1 cut(s)	1472
BstSCI	CCNGG	5 cut(s)	500, 601, 664, 706, 1080
BstSFI	CTRYAG	1 cut(s)	480
BstUI	CGCG	6 cut(s)	300, 1179, 1227, 1301, 1303, 1632
BstV1I	GCAGC	4 cut(s)	1096, 1306, 1309, 1870
BstV2I	GAAGAC	1 cut(s)	819
BstX2I	RGATCY	3 cut(s)	316, 338, 1438
BstXI	CCANNNNNNTGG	1 cut(s)	1804
BstYI	RGATCY	3 cut(s)	316, 338, 1438
Bsu15I	ATCGAT	1 cut(s)	234
BsuRI	GGCC	6 cut(s)	26, 89, 212, 285, 388, 1080
BsuTUI	ATCGAT	1 cut(s)	234
BtgI	CCRYGG	3 cut(s)	213, 342, 1161
BtgZI	GCGATG	1 cut(s)	2066
BtsCI	GGATG	7 cut(s)	461, 511, 631, 646, 1599, 1665, 1984
BtsI	GCAGTG	2 cut(s)	924, 1315
BtsIMutI	CAGTG	6 cut(s)	627, 811, 924, 1315, 1569, 2295
Cac8I	GCNNGC	3 cut(s)	195, 226, 662
CaiI	CAGNNNCTG	1 cut(s)	1112
CfoI	GCGC	3 cut(s)	1303, 1632, 2154
Cfr10I	RCCGGY	1 cut(s)	1485
Cfr13I	GGNCC	3 cut(s)	24, 106, 284
ClaI	ATCGAT	1 cut(s)	234
CseI	GACGC	2 cut(s)	292, 1889
Csp6I	GTAC	5 cut(s)	70, 244, 331, 1663, 2215
CspAI	ACCGGT	1 cut(s)	1485
CspCI	CAANNNNNGTGG	4 cut(s)	603, 638, 1558, 1593
CviQI	GTAC	5 cut(s)	70, 244, 331, 1663, 2215
DdeI	CTNAG	9 cut(s)	170, 240, 327, 531, 771, 1026, 1462, 1531, 1745
DraI	TTTAAA	1 cut(s)	1899
DrdI	GACNNNNNNGTC	1 cut(s)	1457
DseDI	GACNNNNNNGTC	1 cut(s)	1457
EaeI	YGGCCR	2 cut(s)	210, 1078
Eam1104I	CTCTTC	1 cut(s)	708
EarI	CTCTTC	1 cut(s)	708
Ecl136II	GAGCTC	1 cut(s)	43
Eco130I	CCWWGG	3 cut(s)	342, 1161, 1803
Eco24I	GRGCYC	1 cut(s)	45
Eco31I	GGTCTC	1 cut(s)	2013
Eco47I	GGWCC	1 cut(s)	106
Eco47III	AGCGCT	1 cut(s)	2153
Eco53kI	GAGCTC	1 cut(s)	43
Eco57I	CTGAAG	2 cut(s)	831, 1104
EcoICRI	GAGCTC	1 cut(s)	43
EcoRII	CCWGG	4 cut(s)	500, 601, 664, 706
EcoT14I	CCWWGG	3 cut(s)	342, 1161, 1803
EcoT22I	ATGCAT	2 cut(s)	856, 1291
EcoT38I	GRGCYC	1 cut(s)	45
ErhI	CCWWGG	3 cut(s)	342, 1161, 1803
Esp3I	CGTCTC	1 cut(s)	1826
FalI	AAGNNNNNCTT	2 cut(s)	1503, 1535
FauNDI	CATATG	2 cut(s)	1735, 2139
FbaI	TGATCA	1 cut(s)	1216
Fnu4HI	GCNGC	8 cut(s)	27, 112, 210, 301, 1110, 1320, 1323, 1884
FokI	GGATG	7 cut(s)	448, 518, 638, 653, 1606, 1652, 1991
FriOI	GRGCYC	1 cut(s)	45
Fsp4HI	GCNGC	8 cut(s)	27, 112, 210, 301, 1110, 1320, 1323, 1884
FspBI	CTAG	3 cut(s)	1148, 1617, 2085
GlaI	GCGC	3 cut(s)	1302, 1631, 2153
GluI	GCNGC	8 cut(s)	27, 112, 210, 301, 1110, 1320, 1323, 1884
GsuI	CTGGAG	2 cut(s)	1524, 1794
HaeII	RGCGCY	1 cut(s)	2155
HaeIII	GGCC	6 cut(s)	26, 89, 212, 285, 388, 1080
HapII	CCGG	4 cut(s)	426, 1081, 1486, 1591
HgaI	GACGC	2 cut(s)	292, 1889
HhaI	GCGC	3 cut(s)	1303, 1632, 2154
Hin1I	GRCGYC	1 cut(s)	303
Hin6I	GCGC	3 cut(s)	1301, 1630, 2152
HinP1I	GCGC	3 cut(s)	1301, 1630, 2152
HincII	GTYRAC	3 cut(s)	124, 1879, 1926
HindII	GTYRAC	3 cut(s)	124, 1879, 1926
HinfI	GANTC	7 cut(s)	166, 439, 1281, 1388, 1565, 1687, 2207
HpaII	CCGG	4 cut(s)	426, 1081, 1486, 1591
HphI	GGTGA	7 cut(s)	23, 358, 538, 992, 1016, 1520, 2006
Hpy166II	GTNNAC	4 cut(s)	124, 730, 1879, 1926
Hpy188III	TCNNGA	8 cut(s)	443, 742, 1127, 1220, 1385, 1442, 1503, 1533
Hpy8I	GTNNAC	4 cut(s)	124, 730, 1879, 1926
HpyAV	CCTTC	5 cut(s)	61, 894, 907, 1020, 1137
HpyCH4III	ACNGT	8 cut(s)	490, 622, 748, 806, 1261, 1454, 1573, 2290
HpyCH4IV	ACGT	1 cut(s)	68
HpyF10VI	GCNNNNNNNGC	7 cut(s)	108, 156, 583, 1061, 1474, 2149, 2160
HpyF3I	CTNAG	9 cut(s)	170, 240, 327, 531, 771, 1026, 1462, 1531, 1745
HpySE526I	ACGT	1 cut(s)	68
Hsp92I	GRCGYC	1 cut(s)	303
HspAI	GCGC	3 cut(s)	1301, 1630, 2152
Ksp22I	TGATCA	1 cut(s)	1216
LguI	GCTCTTC	1 cut(s)	708
LmnI	GCTCC	4 cut(s)	604, 615, 1060, 1945
Lsp1109I	GCAGC	4 cut(s)	1096, 1306, 1309, 1870
LweI	GCATC	9 cut(s)	196, 841, 1298, 1508, 1513, 1584, 1721, 1741, 2038
MaeI	CTAG	3 cut(s)	1148, 1617, 2085
MaeII	ACGT	1 cut(s)	68
MaeIII	GTNAC	8 cut(s)	304, 364, 526, 766, 980, 1114, 1261, 1861
MbiI	CCGCTC	1 cut(s)	29
MboII	GAAGA	7 cut(s)	26, 71, 695, 824, 1080, 1559, 2088
MfeI	CAATTG	1 cut(s)	2043
MflI	RGATCY	3 cut(s)	316, 338, 1438
MhlI	GDGCHC	1 cut(s)	45
MluCI	AATT	9 cut(s)	871, 1041, 1199, 1554, 1722, 1788, 1841, 2043, 2119
MlyI	GAGTC	3 cut(s)	433, 1382, 1559
MmeI	TCCRAC	1 cut(s)	489
Mph1103I	ATGCAT	2 cut(s)	856, 1291
MseI	TTAA	9 cut(s)	201, 279, 399, 929, 987, 1034, 1890, 1898, 2118
MslI	CAYNNNNRTG	2 cut(s)	989, 995
MspA1I	CMGCKG	1 cut(s)	1109
MspI	CCGG	4 cut(s)	426, 1081, 1486, 1591
MspR9I	CCNGG	5 cut(s)	502, 603, 666, 708, 1082
MunI	CAATTG	1 cut(s)	2043
Mva1269I	GAATGC	1 cut(s)	898
MvaI	CCWGG	4 cut(s)	502, 603, 666, 708
MvnI	CGCG	6 cut(s)	300, 1179, 1227, 1301, 1303, 1632
MwoI	GCNNNNNNNGC	7 cut(s)	108, 156, 583, 1061, 1474, 2149, 2160
NciI	CCSGG	1 cut(s)	1082
NcoI	CCATGG	2 cut(s)	342, 1161
NdeI	CATATG	2 cut(s)	1735, 2139
NlaIV	GGNNCC	5 cut(s)	286, 340, 967, 1159, 1440
NmeAIII	GCCGAG	2 cut(s)	62, 336
NmuCI	GTSAC	4 cut(s)	304, 364, 526, 980
NsiI	ATGCAT	2 cut(s)	856, 1291
NspI	RCATGY	1 cut(s)	1472
PciSI	GCTCTTC	1 cut(s)	708
PcsI	WCGNNNNNNNCGW	1 cut(s)	448
PctI	GAATGC	1 cut(s)	898
PfeI	GAWTC	4 cut(s)	166, 1281, 1687, 2207
PflFI	GACNNNGTC	1 cut(s)	568
PinAI	ACCGGT	1 cut(s)	1485
PkrI	GCNGC	8 cut(s)	28, 113, 211, 302, 1111, 1321, 1324, 1885
PleI	GAGTC	3 cut(s)	433, 1382, 1559
PpsI	GAGTC	3 cut(s)	433, 1382, 1559
PshBI	ATTAAT	1 cut(s)	929
Psp124BI	GAGCTC	1 cut(s)	45
Psp6I	CCWGG	4 cut(s)	500, 601, 664, 706
PspGI	CCWGG	4 cut(s)	500, 601, 664, 706
PspN4I	GGNNCC	5 cut(s)	286, 340, 967, 1159, 1440
PspPI	GGNCC	3 cut(s)	24, 106, 284
PstNI	CAGNNNCTG	1 cut(s)	1112
PsuI	RGATCY	3 cut(s)	316, 338, 1438
PsyI	GACNNNGTC	1 cut(s)	568
PvuII	CAGCTG	1 cut(s)	1109
RsaI	GTAC	5 cut(s)	71, 245, 332, 1664, 2216
RsaNI	GTAC	5 cut(s)	70, 244, 331, 1663, 2215
RseI	CAYNNNNRTG	2 cut(s)	989, 995
SacI	GAGCTC	1 cut(s)	45
SapI	GCTCTTC	1 cut(s)	708
SaqAI	TTAA	9 cut(s)	201, 279, 399, 929, 987, 1034, 1890, 1898, 2118
SatI	GCNGC	8 cut(s)	27, 112, 210, 301, 1110, 1320, 1323, 1884
Sau96I	GGNCC	3 cut(s)	24, 106, 284
ScaI	AGTACT	1 cut(s)	245
SchI	GAGTC	3 cut(s)	433, 1382, 1559
ScrFI	CCNGG	5 cut(s)	502, 603, 666, 708, 1082
SduI	GDGCHC	1 cut(s)	45
SfaNI	GCATC	9 cut(s)	196, 841, 1298, 1508, 1513, 1584, 1721, 1741, 2038
SfcI	CTRYAG	1 cut(s)	480
SinI	GGWCC	1 cut(s)	106
SmiMI	CAYNNNNRTG	2 cut(s)	989, 995
SmlI	CTYRAG	1 cut(s)	2218
SmoI	CTYRAG	1 cut(s)	2218
Sse9I	AATT	9 cut(s)	871, 1041, 1199, 1554, 1722, 1788, 1841, 2043, 2119
SsiI	CCGC	9 cut(s)	27, 34, 100, 109, 112, 209, 300, 1179, 1303
SspI	AATATT	1 cut(s)	2092
SspMI	CTAG	3 cut(s)	1148, 1617, 2085
SstI	GAGCTC	1 cut(s)	45
StyD4I	CCNGG	5 cut(s)	500, 601, 664, 706, 1080
StyI	CCWWGG	3 cut(s)	342, 1161, 1803
TaaI	ACNGT	8 cut(s)	490, 622, 748, 806, 1261, 1454, 1573, 2290
TaiI	ACGT	1 cut(s)	71
TaqI	TCGA	2 cut(s)	234, 355
TasI	AATT	9 cut(s)	871, 1041, 1199, 1554, 1722, 1788, 1841, 2043, 2119
TatI	WGTACW	3 cut(s)	243, 330, 1662
TauI	GCSGC	4 cut(s)	29, 114, 212, 303
TfiI	GAWTC	4 cut(s)	166, 1281, 1687, 2207
Tru1I	TTAA	9 cut(s)	201, 279, 399, 929, 987, 1034, 1890, 1898, 2118
Tru9I	TTAA	9 cut(s)	201, 279, 399, 929, 987, 1034, 1890, 1898, 2118
TscAI	CASTG	6 cut(s)	627, 811, 924, 1315, 1576, 2295
TseFI	GTSAC	4 cut(s)	304, 364, 526, 980
TseI	GCWGC	4 cut(s)	1109, 1319, 1322, 1883
Tsp45I	GTSAC	4 cut(s)	304, 364, 526, 980
TspGWI	ACGGA	2 cut(s)	230, 747
TspRI	CASTG	6 cut(s)	627, 811, 924, 1315, 1576, 2295
Tth111I	GACNNNGTC	1 cut(s)	568
VpaK11BI	GGWCC	1 cut(s)	106
VspI	ATTAAT	1 cut(s)	929
XapI	RAATTY	1 cut(s)	1199
XceI	RCATGY	1 cut(s)	1472
XspI	CTAG	3 cut(s)	1148, 1617, 2085
ZrmI	AGTACT	1 cut(s)	245
Zsp2I	ATGCAT	2 cut(s)	856, 1291

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.