MD05G1301300.v1.1

Olee1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0009996
Physical Location & Seq
Reverse (-)
43422274 .. 43423135
862 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1301300.v1.1.491

Sequence Viewer

Length: 519 bp
ATGGCTAATTCCAATAGTATCGTTCTTGTCTCTGCCCTTTGCTTCTTGTCGCTCGCCGGCGTTGCATACTGCGGCGAGACCCTCAATGTGCACGGCATTGTTTACTGCGACAACTGCCGCATCCAGTTCGTAACCAGAATCAGCGAGACCCTCAAAGGTGCAAAGGTGAGATTGGAATGCAGAGAGAATGAAGGGGGAAAAATAACTCTAAGCAAAGAGGCAGACACCGACGACTTAGGAACTTACTCCATTCCGGTGGAAGGAGACCATGAAGAGGAGGTTTGCGAGGTGATCTTGGTGAAGAGTCCCAAGGAGGACTGCAGCGAGATCAGCAACGAGCTTCATGTGAAGCTCAGCGCGAGGATCAGCCTCACGAATCACAACGGCATCACCGGGCCTCATCGCATGGCGAACCCTCTCGGTTTCATGAAGAAGGAGGTTGATCCTAAGTGCGCTGAGGTCCTCAAGGAGCTCGGACTCACCCCCGATGGCGACATTGATGATAGCAGTGCTGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pollen_Ole_e_I

Protein Analysis

173

Amino Acids

18.67

Weight (kDa)

4.83

Isoelectric Point (pI)

17.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pollen_Ole_e_1 PF01190 30 - 113 8.4e-20 Pollen protein Ole e 1 like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0009996)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G29140 AT4G18596 AT4G18596 AT5G45880
fragaria_vesca FvH4_3g07791
malus_domestica MD05G1301300.v1.1 MD10G1280200.v1.1
prunus_persica Prupe.4G061100_v2.0.a1
pyrus_communis pycom05g27910
rosa_chinensis RchiOBHm_Chr5g0010771
rosa_laevigata RLG00000031778
rosa_multiflora Rmu_sc0000109.1_g000021
rosa_roxburghii Rroxscaffold_1G00065610
rosa_rugosa Rorug04G0452100
rosa_samantha Rh5AG084000 Rh5AG084400 Rh5BG079200 Rh5CG092000 Rh5DG079400
rosa_wichuraiana Rw0G000580 Rw0G022280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 359
AciI CCGC 2 cut(s) 72, 118
AclWI GGATC 2 cut(s) 371, 437
AfiI CCNNNNNNNGG 2 cut(s) 260, 274
AluBI AGCT 3 cut(s) 340, 352, 472
AluI AGCT 3 cut(s) 340, 352, 472
Alw21I GWGCWC 2 cut(s) 93, 474
Alw26I GTCTC 4 cut(s) 34, 71, 140, 258
Alw44I GTGCAC 1 cut(s) 89
AlwI GGATC 2 cut(s) 371, 437
AoxI GGCC 1 cut(s) 395
ApaLI GTGCAC 1 cut(s) 89
ApeKI GCWGC 1 cut(s) 321
AspLEI GCGC 2 cut(s) 359, 455
AspS9I GGNCC 2 cut(s) 395, 460
AsuC2I CCSGG 1 cut(s) 394
AsuHPI GGTGA 5 cut(s) 178, 301, 310, 382, 472
AvaII GGWCC 1 cut(s) 460
BaeGI GKGCMC 1 cut(s) 93
BanII GRGCYC 1 cut(s) 474
Bbv12I GWGCWC 2 cut(s) 93, 474
BbvCI CCTCAGC 1 cut(s) 456
BbvI GCAGC 1 cut(s) 333
BccI CCATC 1 cut(s) 482
BceAI ACGGC 2 cut(s) 109, 400
BcgI CGANNNNNNTGC 2 cut(s) 109, 143
BcnI CCSGG 1 cut(s) 394
BcoDI GTCTC 4 cut(s) 34, 71, 140, 258
BfmI CTRYAG 1 cut(s) 319
BisI GCNGC 3 cut(s) 73, 118, 322
BlpI GCTNAGC 1 cut(s) 353
BlsI GCNGC 3 cut(s) 74, 119, 323
Bme1390I CCNGG 1 cut(s) 394
Bme18I GGWCC 1 cut(s) 460
BmgT120I GGNCC 2 cut(s) 395, 460
BmrFI CCNGG 1 cut(s) 394
BmsI GCATC 2 cut(s) 129, 396
Bpu10I CCTNAGC 1 cut(s) 456
Bpu1102I GCTNAGC 1 cut(s) 353
BpuEI CTTGAG 1 cut(s) 449
BpuMI CCSGG 1 cut(s) 394
BsaI GGTCTC 3 cut(s) 71, 140, 258
BsaJI CCNNGG 1 cut(s) 309
BsaWI WCCGGW 1 cut(s) 253
Bsc4I CCNNNNNNNGG 2 cut(s) 260, 274
Bse118I RCCGGY 1 cut(s) 56
Bse1I ACTGG 1 cut(s) 124
BseDI CCNNGG 1 cut(s) 309
BseGI GGATG 1 cut(s) 120
BseLI CCNNNNNNNGG 2 cut(s) 260, 274
BseMII CTCAG 2 cut(s) 367, 447
BseNI ACTGG 1 cut(s) 124
BseRI GAGGAG 1 cut(s) 290
BseSI GKGCMC 1 cut(s) 93
BseXI GCAGC 1 cut(s) 333
Bsh1236I CGCG 1 cut(s) 359
BshFI GGCC 1 cut(s) 397
BsiHKAI GWGCWC 2 cut(s) 93, 474
BsiSI CCGG 3 cut(s) 57, 254, 393
BslFI GGGAC 1 cut(s) 291
BslI CCNNNNNNNGG 2 cut(s) 260, 274
BsmAI GTCTC 4 cut(s) 34, 71, 140, 258
BsmFI GGGAC 1 cut(s) 291
BsmI GAATGC 1 cut(s) 182
BsnI GGCC 1 cut(s) 397
Bso31I GGTCTC 3 cut(s) 71, 140, 258
Bsp1286I GDGCHC 2 cut(s) 93, 474
Bsp143I GATC 4 cut(s) 291, 327, 363, 442
Bsp1720I GCTNAGC 1 cut(s) 353
BspACI CCGC 2 cut(s) 72, 118
BspANI GGCC 1 cut(s) 397
BspCNI CTCAG 2 cut(s) 366, 448
BspFNI CGCG 1 cut(s) 359
BspHI TCATGA 1 cut(s) 426
BspMAI CTGCAG 1 cut(s) 323
BspPI GGATC 2 cut(s) 371, 437
BspTNI GGTCTC 3 cut(s) 71, 140, 258
BsrFI RCCGGY 1 cut(s) 56
BsrI ACTGG 1 cut(s) 124
BssAI RCCGGY 1 cut(s) 56
BssECI CCNNGG 1 cut(s) 309
BssMI GATC 4 cut(s) 291, 327, 363, 442
BssT1I CCWWGG 1 cut(s) 309
Bst6I CTCTTC 2 cut(s) 267, 296
BstC8I GCNNGC 2 cut(s) 54, 58
BstDEI CTNAG 5 cut(s) 209, 235, 353, 447, 456
BstF5I GGATG 1 cut(s) 120
BstFNI CGCG 1 cut(s) 359
BstHHI GCGC 2 cut(s) 359, 455
BstKTI GATC 4 cut(s) 294, 330, 366, 445
BstMAI GTCTC 4 cut(s) 34, 71, 140, 258
BstMBI GATC 4 cut(s) 291, 327, 363, 442
BstMWI GCNNNNNNNGC 3 cut(s) 62, 114, 330
BstSCI CCNGG 1 cut(s) 392
BstSFI CTRYAG 1 cut(s) 319
BstSLI GKGCMC 1 cut(s) 93
BstUI CGCG 1 cut(s) 359
BstV1I GCAGC 1 cut(s) 333
BstXI CCANNNNNNTGG 1 cut(s) 256
BsuRI GGCC 1 cut(s) 397
BtgZI GCGATG 1 cut(s) 386
BtsCI GGATG 1 cut(s) 120
BtsI GCAGTG 1 cut(s) 514
BtsIMutI CAGTG 1 cut(s) 514
Cac8I GCNNGC 2 cut(s) 54, 58
CciI TCATGA 1 cut(s) 426
CfoI GCGC 2 cut(s) 359, 455
Cfr10I RCCGGY 1 cut(s) 56
Cfr13I GGNCC 2 cut(s) 395, 460
CviAII CATG 4 cut(s) 269, 344, 406, 427
CviJI RGCY 6 cut(s) 5, 340, 352, 369, 397, 472
CviKI_1 RGCY 6 cut(s) 5, 340, 352, 369, 397, 472
DdeI CTNAG 5 cut(s) 209, 235, 353, 447, 456
DpnI GATC 4 cut(s) 293, 329, 365, 444
DpnII GATC 4 cut(s) 291, 327, 363, 442
Eam1104I CTCTTC 2 cut(s) 267, 296
EarI CTCTTC 2 cut(s) 267, 296
Ecl136II GAGCTC 1 cut(s) 472
Eco130I CCWWGG 1 cut(s) 309
Eco24I GRGCYC 1 cut(s) 474
Eco31I GGTCTC 3 cut(s) 71, 140, 258
Eco47I GGWCC 1 cut(s) 460
Eco53kI GAGCTC 1 cut(s) 472
EcoICRI GAGCTC 1 cut(s) 472
EcoO109I RGGNCCY 1 cut(s) 460
EcoT14I CCWWGG 1 cut(s) 309
EcoT38I GRGCYC 1 cut(s) 474
ErhI CCWWGG 1 cut(s) 309
FaeI CATG 4 cut(s) 272, 347, 409, 430
FaiI YATR 5 cut(s) 67, 270, 345, 407, 428
FaqI GGGAC 1 cut(s) 291
FatI CATG 4 cut(s) 268, 343, 405, 426
Fnu4HI GCNGC 3 cut(s) 73, 118, 322
FokI GGATG 1 cut(s) 107
FriOI GRGCYC 1 cut(s) 474
Fsp4HI GCNGC 3 cut(s) 73, 118, 322
GlaI GCGC 2 cut(s) 358, 454
GluI GCNGC 3 cut(s) 73, 118, 322
HaeIII GGCC 1 cut(s) 397
HapII CCGG 3 cut(s) 57, 254, 393
HhaI GCGC 2 cut(s) 359, 455
Hin1II CATG 4 cut(s) 272, 347, 409, 430
Hin6I GCGC 2 cut(s) 357, 453
HinP1I GCGC 2 cut(s) 357, 453
HinfI GANTC 4 cut(s) 138, 304, 376, 477
HpaII CCGG 3 cut(s) 57, 254, 393
HphI GGTGA 5 cut(s) 178, 301, 310, 382, 472
Hpy166II GTNNAC 2 cut(s) 91, 103
Hpy188I TCNGA 1 cut(s) 476
Hpy188III TCNNGA 2 cut(s) 373, 427
Hpy8I GTNNAC 2 cut(s) 91, 103
Hpy99I CGWCG 1 cut(s) 233
HpyAV CCTTC 3 cut(s) 185, 254, 427
HpyCH4V TGCA 5 cut(s) 65, 91, 161, 180, 321
HpyF10VI GCNNNNNNNGC 3 cut(s) 62, 114, 330
HpyF3I CTNAG 5 cut(s) 209, 235, 353, 447, 456
Hsp92II CATG 4 cut(s) 272, 347, 409, 430
HspAI GCGC 2 cut(s) 357, 453
KroI GCCGGC 1 cut(s) 56
KroNI GCCGGC 1 cut(s) 58
Kzo9I GATC 4 cut(s) 291, 327, 363, 442
LmnI GCTCC 1 cut(s) 469
LpnPI CCDG 5 cut(s) 70, 137, 148, 267, 406
Lsp1109I GCAGC 1 cut(s) 333
LweI GCATC 2 cut(s) 129, 396
MaeIII GTNAC 1 cut(s) 130
MalI GATC 4 cut(s) 293, 329, 365, 444
MboI GATC 4 cut(s) 291, 327, 363, 442
MboII GAAGA 3 cut(s) 284, 313, 442
MhlI GDGCHC 2 cut(s) 93, 474
MluCI AATT 1 cut(s) 7
MlyI GAGTC 2 cut(s) 313, 471
MreI CGCCGGCG 1 cut(s) 56
MroNI GCCGGC 1 cut(s) 56
MslI CAYNNNNRTG 1 cut(s) 254
MspI CCGG 3 cut(s) 57, 254, 393
MspR9I CCNGG 1 cut(s) 394
Mva1269I GAATGC 1 cut(s) 182
MvnI CGCG 1 cut(s) 359
MwoI GCNNNNNNNGC 3 cut(s) 62, 114, 330
NaeI GCCGGC 1 cut(s) 58
NciI CCSGG 1 cut(s) 394
NdeII GATC 4 cut(s) 291, 327, 363, 442
NgoMIV GCCGGC 1 cut(s) 56
NlaIII CATG 4 cut(s) 272, 347, 409, 430
PagI TCATGA 1 cut(s) 426
PctI GAATGC 1 cut(s) 182
PdiI GCCGGC 1 cut(s) 58
PfeI GAWTC 2 cut(s) 138, 376
PkrI GCNGC 3 cut(s) 74, 119, 323
PleI GAGTC 2 cut(s) 312, 471
PpsI GAGTC 2 cut(s) 312, 471
PpuMI RGGWCCY 1 cut(s) 460
Psp124BI GAGCTC 1 cut(s) 474
Psp5II RGGWCCY 1 cut(s) 460
PspPI GGNCC 2 cut(s) 395, 460
PspPPI RGGWCCY 1 cut(s) 460
PstI CTGCAG 1 cut(s) 323
RseI CAYNNNNRTG 1 cut(s) 254
SacI GAGCTC 1 cut(s) 474
SatI GCNGC 3 cut(s) 73, 118, 322
Sau3AI GATC 4 cut(s) 291, 327, 363, 442
Sau96I GGNCC 2 cut(s) 395, 460
SchI GAGTC 2 cut(s) 313, 471
ScrFI CCNGG 1 cut(s) 394
SduI GDGCHC 2 cut(s) 93, 474
SetI ASST 9 cut(s) 160, 168, 282, 291, 342, 354, 441, 462, 474
SfaNI GCATC 2 cut(s) 129, 396
SfcI CTRYAG 1 cut(s) 319
SgrAI CRCCGGYG 1 cut(s) 56
SinI GGWCC 1 cut(s) 460
SmiMI CAYNNNNRTG 1 cut(s) 254
SmlI CTYRAG 1 cut(s) 464
SmoI CTYRAG 1 cut(s) 464
Sse9I AATT 1 cut(s) 7
SsiI CCGC 2 cut(s) 72, 118
SstI GAGCTC 1 cut(s) 474
StyD4I CCNGG 1 cut(s) 392
StyI CCWWGG 1 cut(s) 309
TasI AATT 1 cut(s) 7
TauI GCSGC 2 cut(s) 75, 120
TfiI GAWTC 2 cut(s) 138, 376
TscAI CASTG 1 cut(s) 514
TseI GCWGC 1 cut(s) 321
TspDTI ATGAA 5 cut(s) 204, 285, 332, 415, 443
TspRI CASTG 1 cut(s) 514
VneI GTGCAC 1 cut(s) 89
VpaK11BI GGWCC 1 cut(s) 460
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.