RLG00000031778

Olee1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0009996
Physical Location & Seq
Forward (+)
8170553 .. 8171143
591 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000031778

Sequence Viewer

Length: 510 bp
ATGGCCAAGTCTAGTATTATCATCCTTGCTTCAGCCCTTTGCTTCTTGTCCTTGATCGACAACGCTTACAGCGAAGACAAGACAACACTCCAGGTTGAAGGCGTAGTCTTTTGTGACAGCTGCCGCATCCAGTTCATCACCAGAATCAGCGAGCTTATACAAGGTGCAAAGGTGAAGTTGGAATGCAAGGACCGCGAAAGTGGTAGCATAACGTACAGCAAGGAGGCCGAGACCAACGAGCAAGGAAGGTACTCAATTGAAGTGGATAGTGACCATGAGGAGGAGGATTGCGAGGTCGTCCTGGTGAGCAGCCCCAACGAGGACTGCAACGAGATTAACCACGACTTACACCAACAGACCAGCGCGAGGATTAGTCTCACAAAACACAATGGAATTGCCGGACCGGTGCGCATTCCGAACCCTCTTGGCTTCATGAAGAAGGAAGCTGCTCCGGAGTGCCCTGAGGTGCTCAAGGAGCTTGGACTCACCCCGGATGGCGACATCGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pollen_Ole_e_I

Protein Analysis

170

Amino Acids

18.64

Weight (kDa)

4.61

Isoelectric Point (pI)

46.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pollen_Ole_e_1 PF01190 32 - 115 1.8e-21 Pollen protein Ole e 1 like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0009996)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G29140 AT4G18596 AT4G18596 AT5G45880
fragaria_vesca FvH4_3g07791
malus_domestica MD05G1301300.v1.1 MD10G1280200.v1.1
prunus_persica Prupe.4G061100_v2.0.a1
pyrus_communis pycom05g27910
rosa_chinensis RchiOBHm_Chr5g0010771
rosa_laevigata RLG00000031778
rosa_multiflora Rmu_sc0000109.1_g000021
rosa_roxburghii Rroxscaffold_1G00065610
rosa_rugosa Rorug04G0452100
rosa_samantha Rh5AG084000 Rh5AG084400 Rh5BG079200 Rh5CG092000 Rh5DG079400
rosa_wichuraiana Rw0G000580 Rw0G022280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 410
AccII CGCG 2 cut(s) 195, 365
AccIII TCCGGA 1 cut(s) 451
AciI CCGC 2 cut(s) 124, 193
AcoI YGGCCR 1 cut(s) 3
AcuI CTGAAG 1 cut(s) 15
AfaI GTAC 2 cut(s) 215, 251
AfiI CCNNNNNNNGG 3 cut(s) 280, 319, 366
AgeI ACCGGT 1 cut(s) 403
AgsI TTSAA 2 cut(s) 98, 260
AjnI CCWGG 2 cut(s) 90, 300
AluBI AGCT 4 cut(s) 120, 154, 446, 478
AluI AGCT 4 cut(s) 120, 154, 446, 478
Alw21I GWGCWC 1 cut(s) 471
Alw26I GTCTC 2 cut(s) 224, 380
Aor13HI TCCGGA 1 cut(s) 451
AoxI GGCC 2 cut(s) 3, 225
ApeKI GCWGC 3 cut(s) 120, 309, 446
AsiGI ACCGGT 1 cut(s) 403
AspLEI GCGC 2 cut(s) 365, 411
AspS9I GGNCC 2 cut(s) 190, 401
AsuC2I CCSGG 1 cut(s) 491
AsuHPI GGTGA 4 cut(s) 130, 184, 316, 478
AvaII GGWCC 2 cut(s) 190, 401
AxyI CCTNAGG 1 cut(s) 462
BaeGI GKGCMC 1 cut(s) 461
BalI TGGCCA 1 cut(s) 5
BbsI GAAGAC 1 cut(s) 81
Bbv12I GWGCWC 1 cut(s) 471
BbvI GCAGC 3 cut(s) 107, 321, 433
BccI CCATC 1 cut(s) 488
BciT130I CCWGG 2 cut(s) 92, 302
BcnI CCSGG 1 cut(s) 491
BcoDI GTCTC 2 cut(s) 224, 380
BfaI CTAG 1 cut(s) 12
BisI GCNGC 4 cut(s) 121, 124, 310, 447
BlsI GCNGC 4 cut(s) 122, 125, 311, 448
Bme1390I CCNGG 3 cut(s) 92, 302, 491
Bme18I GGWCC 2 cut(s) 190, 401
BmgT120I GGNCC 2 cut(s) 190, 401
BmrFI CCNGG 3 cut(s) 92, 302, 491
BmsI GCATC 1 cut(s) 135
BpiI GAAGAC 1 cut(s) 81
BpmI CTGGAG 1 cut(s) 74
BpuEI CTTGAG 1 cut(s) 455
BpuMI CCSGG 1 cut(s) 491
BsaI GGTCTC 1 cut(s) 224
BsaJI CCNNGG 1 cut(s) 489
BsaWI WCCGGW 2 cut(s) 403, 451
Bsc4I CCNNNNNNNGG 3 cut(s) 280, 319, 366
Bse118I RCCGGY 1 cut(s) 403
Bse1I ACTGG 1 cut(s) 130
Bse21I CCTNAGG 1 cut(s) 462
BseAI TCCGGA 1 cut(s) 451
BseBI CCWGG 2 cut(s) 92, 302
BseDI CCNNGG 1 cut(s) 489
BseGI GGATG 3 cut(s) 21, 126, 499
BseLI CCNNNNNNNGG 3 cut(s) 280, 319, 366
BseMII CTCAG 1 cut(s) 453
BseNI ACTGG 1 cut(s) 130
BseRI GAGGAG 2 cut(s) 293, 296
BseSI GKGCMC 1 cut(s) 461
BseXI GCAGC 3 cut(s) 107, 321, 433
Bsh1236I CGCG 2 cut(s) 195, 365
BshFI GGCC 2 cut(s) 5, 227
BshTI ACCGGT 1 cut(s) 403
BsiHKAI GWGCWC 1 cut(s) 471
BsiSI CCGG 4 cut(s) 399, 404, 452, 491
BslI CCNNNNNNNGG 3 cut(s) 280, 319, 366
BsmAI GTCTC 2 cut(s) 224, 380
BsmI GAATGC 2 cut(s) 188, 411
BsnI GGCC 2 cut(s) 5, 227
Bso31I GGTCTC 1 cut(s) 224
Bsp1286I GDGCHC 2 cut(s) 461, 471
Bsp13I TCCGGA 1 cut(s) 451
Bsp143I GATC 1 cut(s) 54
BspACI CCGC 2 cut(s) 124, 193
BspANI GGCC 2 cut(s) 5, 227
BspCNI CTCAG 1 cut(s) 454
BspEI TCCGGA 1 cut(s) 451
BspFNI CGCG 2 cut(s) 195, 365
BspHI TCATGA 1 cut(s) 432
BspTNI GGTCTC 1 cut(s) 224
BsrFI RCCGGY 1 cut(s) 403
BsrI ACTGG 1 cut(s) 130
BssAI RCCGGY 1 cut(s) 403
BssECI CCNNGG 1 cut(s) 489
BssMI GATC 1 cut(s) 54
Bst2UI CCWGG 2 cut(s) 92, 302
BstC8I GCNNGC 1 cut(s) 152
BstDEI CTNAG 1 cut(s) 462
BstF5I GGATG 3 cut(s) 21, 126, 499
BstFNI CGCG 2 cut(s) 195, 365
BstHHI GCGC 2 cut(s) 365, 411
BstKTI GATC 1 cut(s) 57
BstMAI GTCTC 2 cut(s) 224, 380
BstMBI GATC 1 cut(s) 54
BstMWI GCNNNNNNNGC 2 cut(s) 192, 475
BstNI CCWGG 2 cut(s) 92, 302
BstSCI CCNGG 3 cut(s) 90, 300, 489
BstSLI GKGCMC 1 cut(s) 461
BstUI CGCG 2 cut(s) 195, 365
BstV1I GCAGC 3 cut(s) 107, 321, 433
BstV2I GAAGAC 1 cut(s) 81
Bsu36I CCTNAGG 1 cut(s) 462
BsuRI GGCC 2 cut(s) 5, 227
BtsCI GGATG 3 cut(s) 21, 126, 499
Cac8I GCNNGC 1 cut(s) 152
CciI TCATGA 1 cut(s) 432
CfoI GCGC 2 cut(s) 365, 411
Cfr10I RCCGGY 1 cut(s) 403
Cfr13I GGNCC 2 cut(s) 190, 401
CpoI CGGWCCG 1 cut(s) 401
Csp6I GTAC 2 cut(s) 214, 250
CspAI ACCGGT 1 cut(s) 403
CspCI CAANNNNNGTGG 2 cut(s) 243, 278
CspI CGGWCCG 1 cut(s) 401
CviAII CATG 2 cut(s) 275, 433
CviJI RGCY 9 cut(s) 5, 35, 120, 154, 227, 312, 429, 446, 478
CviKI_1 RGCY 9 cut(s) 5, 35, 120, 154, 227, 312, 429, 446, 478
CviQI GTAC 2 cut(s) 214, 250
DdeI CTNAG 1 cut(s) 462
DpnI GATC 1 cut(s) 56
DpnII GATC 1 cut(s) 54
EaeI YGGCCR 1 cut(s) 3
Eco31I GGTCTC 1 cut(s) 224
Eco47I GGWCC 2 cut(s) 190, 401
Eco57I CTGAAG 1 cut(s) 15
Eco81I CCTNAGG 1 cut(s) 462
EcoRII CCWGG 2 cut(s) 90, 300
FaeI CATG 2 cut(s) 278, 436
FaiI YATR 4 cut(s) 158, 209, 276, 434
FatI CATG 2 cut(s) 274, 432
Fnu4HI GCNGC 4 cut(s) 121, 124, 310, 447
FokI GGATG 3 cut(s) 8, 113, 506
Fsp4HI GCNGC 4 cut(s) 121, 124, 310, 447
FspAI RTGCGCAY 1 cut(s) 410
FspBI CTAG 1 cut(s) 12
FspI TGCGCA 1 cut(s) 410
GlaI GCGC 2 cut(s) 364, 410
GluI GCNGC 4 cut(s) 121, 124, 310, 447
GsuI CTGGAG 1 cut(s) 74
HaeIII GGCC 2 cut(s) 5, 227
HapII CCGG 4 cut(s) 399, 404, 452, 491
HhaI GCGC 2 cut(s) 365, 411
Hin1II CATG 2 cut(s) 278, 436
Hin6I GCGC 2 cut(s) 363, 409
HinP1I GCGC 2 cut(s) 363, 409
HinfI GANTC 2 cut(s) 144, 483
HpaII CCGG 4 cut(s) 399, 404, 452, 491
HphI GGTGA 4 cut(s) 130, 184, 316, 478
Hpy188I TCNGA 1 cut(s) 417
Hpy188III TCNNGA 2 cut(s) 433, 452
HpyAV CCTTC 3 cut(s) 92, 240, 433
HpyCH4IV ACGT 1 cut(s) 212
HpyCH4V TGCA 3 cut(s) 167, 186, 327
HpyF10VI GCNNNNNNNGC 2 cut(s) 192, 475
HpyF3I CTNAG 1 cut(s) 462
HpySE526I ACGT 1 cut(s) 212
Hsp92II CATG 2 cut(s) 278, 436
HspAI GCGC 2 cut(s) 363, 409
Kpn2I TCCGGA 1 cut(s) 451
Kzo9I GATC 1 cut(s) 54
LmnI GCTCC 2 cut(s) 454, 475
Lsp1109I GCAGC 3 cut(s) 107, 321, 433
LweI GCATC 1 cut(s) 135
MaeI CTAG 1 cut(s) 12
MaeII ACGT 1 cut(s) 212
MaeIII GTNAC 2 cut(s) 113, 269
MalI GATC 1 cut(s) 56
MboI GATC 1 cut(s) 54
MboII GAAGA 2 cut(s) 86, 448
MfeI CAATTG 1 cut(s) 255
MhlI GDGCHC 2 cut(s) 461, 471
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 2 cut(s) 255, 393
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 1 cut(s) 477
MmeI TCCRAC 1 cut(s) 159
MnlI CCTC 9 cut(s) 217, 271, 274, 277, 286, 313, 360, 432, 457
Mox20I TGGCCA 1 cut(s) 5
MroI TCCGGA 1 cut(s) 451
MscI TGGCCA 1 cut(s) 5
MseI TTAA 1 cut(s) 336
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 1 cut(s) 120
MspI CCGG 4 cut(s) 399, 404, 452, 491
MspR9I CCNGG 3 cut(s) 92, 302, 491
MunI CAATTG 1 cut(s) 255
Mva1269I GAATGC 2 cut(s) 188, 411
MvaI CCWGG 2 cut(s) 92, 302
MvnI CGCG 2 cut(s) 195, 365
MwoI GCNNNNNNNGC 2 cut(s) 192, 475
NciI CCSGG 1 cut(s) 491
NdeII GATC 1 cut(s) 54
NlaIII CATG 2 cut(s) 278, 436
NmeAIII GCCGAG 1 cut(s) 253
NmuCI GTSAC 2 cut(s) 113, 269
NsbI TGCGCA 1 cut(s) 410
PagI TCATGA 1 cut(s) 432
PcsI WCGNNNNNNNCGW 1 cut(s) 69
PctI GAATGC 2 cut(s) 188, 411
PfeI GAWTC 1 cut(s) 144
PinAI ACCGGT 1 cut(s) 403
PkrI GCNGC 4 cut(s) 122, 125, 311, 448
PleI GAGTC 1 cut(s) 477
PpsI GAGTC 1 cut(s) 477
Psp6I CCWGG 2 cut(s) 90, 300
PspGI CCWGG 2 cut(s) 90, 300
PspPI GGNCC 2 cut(s) 190, 401
PvuII CAGCTG 1 cut(s) 120
RsaI GTAC 2 cut(s) 215, 251
RsaNI GTAC 2 cut(s) 214, 250
Rsr2I CGGWCCG 1 cut(s) 401
RsrII CGGWCCG 1 cut(s) 401
SaqAI TTAA 1 cut(s) 336
SatI GCNGC 4 cut(s) 121, 124, 310, 447
Sau3AI GATC 1 cut(s) 54
Sau96I GGNCC 2 cut(s) 190, 401
SchI GAGTC 1 cut(s) 477
ScrFI CCNGG 3 cut(s) 92, 302, 491
SduI GDGCHC 2 cut(s) 461, 471
SfaNI GCATC 1 cut(s) 135
SinI GGWCC 2 cut(s) 190, 401
SmlI CTYRAG 1 cut(s) 470
SmoI CTYRAG 1 cut(s) 470
Sse9I AATT 2 cut(s) 255, 393
SsiI CCGC 2 cut(s) 124, 193
SspMI CTAG 1 cut(s) 12
StyD4I CCNGG 3 cut(s) 90, 300, 489
TaiI ACGT 1 cut(s) 215
TaqI TCGA 2 cut(s) 57, 504
TasI AATT 2 cut(s) 255, 393
TauI GCSGC 1 cut(s) 126
TfiI GAWTC 1 cut(s) 144
Tru1I TTAA 1 cut(s) 336
Tru9I TTAA 1 cut(s) 336
TseFI GTSAC 2 cut(s) 113, 269
TseI GCWGC 3 cut(s) 120, 309, 446
Tsp45I GTSAC 2 cut(s) 113, 269
TspDTI ATGAA 3 cut(s) 124, 421, 449
VpaK11BI GGWCC 2 cut(s) 190, 401
XspI CTAG 1 cut(s) 12
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.