pycom05g27910

Olee1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0009996
Physical Location & Seq
Reverse (-)
28780574 .. 28781542
969 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g27910.1

Sequence Viewer

Length: 597 bp
ATGCTCAAGATGAAGGAATTCTGCTCTGGCATGTCTCTTTTATTGAACAAAAAAAAAAACGCGAAATTTAACCTGACGATGGCTAATTCTAATAGTATCGTTCTTGTCTCTGCCCTTTGCTTCTTGTCGCTCGTCGGCGTCGCCTACTGCGGCGAGACCCTCAATGTGCACGGCATTGTTTACTGCGACAACTGCCGCATCCAATTCGTAACCAGAATCAGCGAGACCCTCAAAGGTGCAAAGGTGAGATTGGAATGCAGAGAGAATGAAGGGGGGAAAATAACTCTAAGCAAAGAGGCAGACACCGACGACTTAGGAACTTACTCCATTCCGGTGGAAGGAGACCATGAAGAGGAGGTTTGCGAGGTGATCTTGGTGAAGAGTCCCAAGGAGGACTGCAGCGAGATCAGCAACGAGCTTCATGTGAAGCTCAGCGCGAGGATCAGTCTCACGAACCACAACGGCATCACCGGGCCTCATCGCATGGCGAACCCTCTCGGTTTCATGAAGAAGGAGGTTGATCCTAAGTGCGCTGAGGTCCTCAAGGAGCTCGGACTCACCCCCGATGGCGACATTGATGATGGCGGTGCTGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pollen_Ole_e_I

Protein Analysis

199

Amino Acids

21.63

Weight (kDa)

5.33

Isoelectric Point (pI)

19.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pollen_Ole_e_1 PF01190 56 - 139 1.2e-19 Pollen protein Ole e 1 like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0009996)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G29140 AT4G18596 AT4G18596 AT5G45880
fragaria_vesca FvH4_3g07791
malus_domestica MD05G1301300.v1.1 MD10G1280200.v1.1
prunus_persica Prupe.4G061100_v2.0.a1
pyrus_communis pycom05g27910
rosa_chinensis RchiOBHm_Chr5g0010771
rosa_laevigata RLG00000031778
rosa_multiflora Rmu_sc0000109.1_g000021
rosa_roxburghii Rroxscaffold_1G00065610
rosa_rugosa Rorug04G0452100
rosa_samantha Rh5AG084000 Rh5AG084400 Rh5BG079200 Rh5CG092000 Rh5DG079400
rosa_wichuraiana Rw0G000580 Rw0G022280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 62, 437
AciI CCGC 3 cut(s) 150, 196, 585
AclWI GGATC 2 cut(s) 449, 515
AcsI RAATTY 2 cut(s) 17, 65
AcyI GRCGYC 1 cut(s) 138
AfiI CCNNNNNNNGG 3 cut(s) 79, 338, 352
AgsI TTSAA 1 cut(s) 46
AluBI AGCT 3 cut(s) 418, 430, 550
AluI AGCT 3 cut(s) 418, 430, 550
Alw21I GWGCWC 2 cut(s) 171, 552
Alw26I GTCTC 6 cut(s) 39, 112, 149, 218, 336, 452
Alw44I GTGCAC 1 cut(s) 167
AlwI GGATC 2 cut(s) 449, 515
AoxI GGCC 1 cut(s) 473
ApaLI GTGCAC 1 cut(s) 167
ApeKI GCWGC 1 cut(s) 399
ApoI RAATTY 2 cut(s) 17, 65
Asp700I GAANNNNTTC 1 cut(s) 17
AspLEI GCGC 2 cut(s) 437, 533
AspS9I GGNCC 2 cut(s) 473, 538
AsuC2I CCSGG 1 cut(s) 472
AsuHPI GGTGA 5 cut(s) 256, 379, 388, 460, 550
AvaII GGWCC 1 cut(s) 538
BaeGI GKGCMC 1 cut(s) 171
BanII GRGCYC 1 cut(s) 552
Bbv12I GWGCWC 2 cut(s) 171, 552
BbvCI CCTCAGC 1 cut(s) 534
BbvI GCAGC 1 cut(s) 411
BccI CCATC 3 cut(s) 73, 560, 575
BceAI ACGGC 2 cut(s) 187, 478
BcgI CGANNNNNNTGC 2 cut(s) 187, 221
BcnI CCSGG 1 cut(s) 472
BcoDI GTCTC 6 cut(s) 39, 112, 149, 218, 336, 452
BfmI CTRYAG 1 cut(s) 397
BisI GCNGC 3 cut(s) 151, 196, 400
BlpI GCTNAGC 1 cut(s) 431
BlsI GCNGC 3 cut(s) 152, 197, 401
Bme1390I CCNGG 1 cut(s) 472
Bme18I GGWCC 1 cut(s) 538
BmgT120I GGNCC 2 cut(s) 473, 538
BmrFI CCNGG 1 cut(s) 472
BmsI GCATC 2 cut(s) 207, 474
Bpu10I CCTNAGC 1 cut(s) 534
Bpu1102I GCTNAGC 1 cut(s) 431
BpuEI CTTGAG 1 cut(s) 527
BpuMI CCSGG 1 cut(s) 472
BsaHI GRCGYC 1 cut(s) 138
BsaI GGTCTC 3 cut(s) 149, 218, 336
BsaJI CCNNGG 1 cut(s) 387
BsaWI WCCGGW 1 cut(s) 331
Bsc4I CCNNNNNNNGG 3 cut(s) 79, 338, 352
BseDI CCNNGG 1 cut(s) 387
BseGI GGATG 1 cut(s) 198
BseLI CCNNNNNNNGG 3 cut(s) 79, 338, 352
BseMII CTCAG 2 cut(s) 445, 525
BseRI GAGGAG 1 cut(s) 368
BseSI GKGCMC 1 cut(s) 171
BseXI GCAGC 1 cut(s) 411
Bsh1236I CGCG 2 cut(s) 62, 437
BshFI GGCC 1 cut(s) 475
BsiHKAI GWGCWC 2 cut(s) 171, 552
BsiSI CCGG 2 cut(s) 332, 471
BslFI GGGAC 1 cut(s) 369
BslI CCNNNNNNNGG 3 cut(s) 79, 338, 352
BsmAI GTCTC 6 cut(s) 39, 112, 149, 218, 336, 452
BsmFI GGGAC 1 cut(s) 369
BsmI GAATGC 1 cut(s) 260
BsnI GGCC 1 cut(s) 475
Bso31I GGTCTC 3 cut(s) 149, 218, 336
Bsp1286I GDGCHC 2 cut(s) 171, 552
Bsp143I GATC 4 cut(s) 369, 405, 441, 520
Bsp1720I GCTNAGC 1 cut(s) 431
BspACI CCGC 3 cut(s) 150, 196, 585
BspANI GGCC 1 cut(s) 475
BspCNI CTCAG 2 cut(s) 444, 526
BspFNI CGCG 2 cut(s) 62, 437
BspHI TCATGA 1 cut(s) 504
BspMAI CTGCAG 1 cut(s) 401
BspPI GGATC 2 cut(s) 449, 515
BspTNI GGTCTC 3 cut(s) 149, 218, 336
BssECI CCNNGG 1 cut(s) 387
BssMI GATC 4 cut(s) 369, 405, 441, 520
BssNI GRCGYC 1 cut(s) 138
BssT1I CCWWGG 1 cut(s) 387
Bst6I CTCTTC 2 cut(s) 345, 374
BstACI GRCGYC 1 cut(s) 138
BstDEI CTNAG 5 cut(s) 287, 313, 431, 525, 534
BstF5I GGATG 1 cut(s) 198
BstFNI CGCG 2 cut(s) 62, 437
BstHHI GCGC 2 cut(s) 437, 533
BstKTI GATC 4 cut(s) 372, 408, 444, 523
BstMAI GTCTC 6 cut(s) 39, 112, 149, 218, 336, 452
BstMBI GATC 4 cut(s) 369, 405, 441, 520
BstMWI GCNNNNNNNGC 2 cut(s) 192, 408
BstNSI RCATGY 1 cut(s) 34
BstSCI CCNGG 1 cut(s) 470
BstSFI CTRYAG 1 cut(s) 397
BstSLI GKGCMC 1 cut(s) 171
BstUI CGCG 2 cut(s) 62, 437
BstV1I GCAGC 1 cut(s) 411
BstXI CCANNNNNNTGG 1 cut(s) 334
BsuRI GGCC 1 cut(s) 475
BtgZI GCGATG 1 cut(s) 464
BtsCI GGATG 1 cut(s) 198
CciI TCATGA 1 cut(s) 504
CfoI GCGC 2 cut(s) 437, 533
Cfr13I GGNCC 2 cut(s) 473, 538
CseI GACGC 1 cut(s) 127
CviAII CATG 5 cut(s) 31, 347, 422, 484, 505
CviJI RGCY 5 cut(s) 83, 418, 430, 475, 550
CviKI_1 RGCY 5 cut(s) 83, 418, 430, 475, 550
DdeI CTNAG 5 cut(s) 287, 313, 431, 525, 534
DpnI GATC 4 cut(s) 371, 407, 443, 522
DpnII GATC 4 cut(s) 369, 405, 441, 520
Eam1104I CTCTTC 2 cut(s) 345, 374
EarI CTCTTC 2 cut(s) 345, 374
Ecl136II GAGCTC 1 cut(s) 550
Eco130I CCWWGG 1 cut(s) 387
Eco24I GRGCYC 1 cut(s) 552
Eco31I GGTCTC 3 cut(s) 149, 218, 336
Eco47I GGWCC 1 cut(s) 538
Eco53kI GAGCTC 1 cut(s) 550
EcoICRI GAGCTC 1 cut(s) 550
EcoO109I RGGNCCY 1 cut(s) 538
EcoRI GAATTC 1 cut(s) 17
EcoT14I CCWWGG 1 cut(s) 387
EcoT38I GRGCYC 1 cut(s) 552
ErhI CCWWGG 1 cut(s) 387
FaeI CATG 5 cut(s) 34, 350, 425, 487, 508
FaiI YATR 5 cut(s) 32, 348, 423, 485, 506
FaqI GGGAC 1 cut(s) 369
FatI CATG 5 cut(s) 30, 346, 421, 483, 504
Fnu4HI GCNGC 3 cut(s) 151, 196, 400
FokI GGATG 1 cut(s) 185
FriOI GRGCYC 1 cut(s) 552
Fsp4HI GCNGC 3 cut(s) 151, 196, 400
GlaI GCGC 2 cut(s) 436, 532
GluI GCNGC 3 cut(s) 151, 196, 400
HaeIII GGCC 1 cut(s) 475
HapII CCGG 2 cut(s) 332, 471
HgaI GACGC 1 cut(s) 127
HhaI GCGC 2 cut(s) 437, 533
Hin1I GRCGYC 1 cut(s) 138
Hin1II CATG 5 cut(s) 34, 350, 425, 487, 508
Hin6I GCGC 2 cut(s) 435, 531
HinP1I GCGC 2 cut(s) 435, 531
HinfI GANTC 3 cut(s) 216, 382, 555
HpaII CCGG 2 cut(s) 332, 471
HphI GGTGA 5 cut(s) 256, 379, 388, 460, 550
Hpy166II GTNNAC 2 cut(s) 169, 181
Hpy188I TCNGA 1 cut(s) 554
Hpy188III TCNNGA 3 cut(s) 7, 451, 505
Hpy8I GTNNAC 2 cut(s) 169, 181
Hpy99I CGWCG 3 cut(s) 137, 143, 311
HpyAV CCTTC 4 cut(s) 7, 263, 332, 505
HpyCH4V TGCA 4 cut(s) 169, 239, 258, 399
HpyF10VI GCNNNNNNNGC 2 cut(s) 192, 408
HpyF3I CTNAG 5 cut(s) 287, 313, 431, 525, 534
Hsp92I GRCGYC 1 cut(s) 138
Hsp92II CATG 5 cut(s) 34, 350, 425, 487, 508
HspAI GCGC 2 cut(s) 435, 531
Kzo9I GATC 4 cut(s) 369, 405, 441, 520
LmnI GCTCC 1 cut(s) 547
LpnPI CCDG 5 cut(s) 12, 86, 226, 345, 484
Lsp1109I GCAGC 1 cut(s) 411
LweI GCATC 2 cut(s) 207, 474
MaeIII GTNAC 1 cut(s) 208
MalI GATC 4 cut(s) 371, 407, 443, 522
MboI GATC 4 cut(s) 369, 405, 441, 520
MboII GAAGA 3 cut(s) 362, 391, 520
MhlI GDGCHC 2 cut(s) 171, 552
MluCI AATT 4 cut(s) 17, 65, 85, 203
MlyI GAGTC 2 cut(s) 391, 549
MroXI GAANNNNTTC 1 cut(s) 17
MseI TTAA 1 cut(s) 69
MslI CAYNNNNRTG 1 cut(s) 332
MspI CCGG 2 cut(s) 332, 471
MspR9I CCNGG 1 cut(s) 472
Mva1269I GAATGC 1 cut(s) 260
MvnI CGCG 2 cut(s) 62, 437
MwoI GCNNNNNNNGC 2 cut(s) 192, 408
NciI CCSGG 1 cut(s) 472
NdeII GATC 4 cut(s) 369, 405, 441, 520
NlaIII CATG 5 cut(s) 34, 350, 425, 487, 508
NspI RCATGY 1 cut(s) 34
PagI TCATGA 1 cut(s) 504
PctI GAATGC 1 cut(s) 260
PdmI GAANNNNTTC 1 cut(s) 17
PfeI GAWTC 1 cut(s) 216
PkrI GCNGC 3 cut(s) 152, 197, 401
PleI GAGTC 2 cut(s) 390, 549
PpsI GAGTC 2 cut(s) 390, 549
PpuMI RGGWCCY 1 cut(s) 538
Psp124BI GAGCTC 1 cut(s) 552
Psp5II RGGWCCY 1 cut(s) 538
PspPI GGNCC 2 cut(s) 473, 538
PspPPI RGGWCCY 1 cut(s) 538
PstI CTGCAG 1 cut(s) 401
RseI CAYNNNNRTG 1 cut(s) 332
SacI GAGCTC 1 cut(s) 552
SaqAI TTAA 1 cut(s) 69
SatI GCNGC 3 cut(s) 151, 196, 400
Sau3AI GATC 4 cut(s) 369, 405, 441, 520
Sau96I GGNCC 2 cut(s) 473, 538
SchI GAGTC 2 cut(s) 391, 549
ScrFI CCNGG 1 cut(s) 472
SduI GDGCHC 2 cut(s) 171, 552
SfaNI GCATC 2 cut(s) 207, 474
SfcI CTRYAG 1 cut(s) 397
SinI GGWCC 1 cut(s) 538
SmiMI CAYNNNNRTG 1 cut(s) 332
SmlI CTYRAG 2 cut(s) 5, 542
SmoI CTYRAG 2 cut(s) 5, 542
Sse9I AATT 4 cut(s) 17, 65, 85, 203
SsiI CCGC 3 cut(s) 150, 196, 585
SstI GAGCTC 1 cut(s) 552
StyD4I CCNGG 1 cut(s) 470
StyI CCWWGG 1 cut(s) 387
TasI AATT 4 cut(s) 17, 65, 85, 203
TauI GCSGC 2 cut(s) 153, 198
TfiI GAWTC 1 cut(s) 216
Tru1I TTAA 1 cut(s) 69
Tru9I TTAA 1 cut(s) 69
TseI GCWGC 1 cut(s) 399
TspDTI ATGAA 6 cut(s) 26, 282, 363, 410, 493, 521
VneI GTGCAC 1 cut(s) 167
VpaK11BI GGWCC 1 cut(s) 538
XapI RAATTY 2 cut(s) 17, 65
XceI RCATGY 1 cut(s) 34
XmnI GAANNNNTTC 1 cut(s) 17
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.