Rorug04G0452100

Putative serine esterase (DUF676)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0004001
Physical Location & Seq
Reverse (-)
60675874 .. 60679726
3853 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0452100.1

Sequence Viewer

Length: 1425 bp
ATGGCGGCTTCCACGAAATCGCTTAAAGAGTATTTGAAACGATACGAATCTAGTAATAACGAGGACGAGACGAAGAAGAAGAGGAAGAAGAAGCAGAAGAGCAAGGCGGAGGCAATGGGGGTTAAAGTGGTAGATGAAGACCCTGTGTGGCAGAAACCGGTGAAAGCTGAGAGAGAAGATGATAACAATTCAGCTGACGAAGAGGAGAAGCCTTTAGTTGATGAAGACATTGAGGTGAAGAGGATGAAGAGACTTGAGTTATTAAGGGCTCGGCGTCCCTATGATGCTATATCTGAAGATGGCAGTGGTTGGGTTAAGGTCCCTCTGAAACGAGAAGGCTCCGAAGAGGCTGACTTGTCACCTCCGCGGAAGCGCTTTCATTCTCCCTCACCTGAGCCTGGAATGACTTCGAGGAAAAGTAGGAGGGGTGGTGATGAAGATCTTTCTCCACCGAGGAAGAGTAGGAACAATGTTGAAGATCTTTCTCCACCGAGGAAGAGTAGGAACAATGTTGAAGATCTTTCTCCACCGAGGAAGAGGAGGAACAATGTTGCGGATCTTTCACCACCGAGGAAGAGTAGGAACAATGGTGATGGAGATCTTTCTCCACCAAGGAAGAGTCGGAACATTGTTGCAGATCTTTCAATACCAAGGAAGAGAAGGAACAATGGTGATGCAGATCTTTCTCCACCAAGGAAAAGTAGGAGGGGTATTGATGAAGATCTATCTCCACCAAGGAAAAAGAAGCAAGTATCTCCGGGTCCTTTGAAAGAGAAGCCAAAAAGTGGTTTGATATCTGGCCGAGATATCAGCGAAGAGATTTCTAAAACAAAGAGAGCTGAGCAGTCGAGATTTGAACAGATGGATCCTTCTGAAACTGGGCAACAAGCAGCAGTTGTATTTCGTAATAAGAAAACAGGAGAACGCATGTCGGAGGAGGAGCGATTGAAATCAGAAAAGAAACTTGATGAAAAGCCTAAGGAGATAAAGTTGGAATGGGGTAAGGGCTTGGCTCAAAAGCGGGAAGCTGAAGCTAAGCTAGAGGAAATAGAACGTGAGAAGGAAAAACCATTTGCACGGACTAGAGATGATCCAGAACTTGACAAAATGATGAAGGAGAGAGTAAGATGGGGTGACCCAATGGCACATTTGGTAAAGAAAAAGTATCCTGAAGCAGTTCTCCCTAACCTAGGGGATAGCGAGCAAATGAAGGAATCTGGATTTATTATCCCTCAGGATATTCCTAATCATAGCTGGTTAAAAAGAGGATTAGATGCTGCACCAAATCGATATGGTATAAAACCAGGGAGACATTGGGATGGAGTTGATCGCAGTAACGGATTTGAGAAAGGATTGTTCAAACGGACTAACGAGAAACAAGCTACTCAGACGGAGGCTTATCTTTGGTCTGTCGCAGATATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF676

Protein Analysis

474

Amino Acids

54.39

Weight (kDa)

9.63

Isoelectric Point (pI)

64.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Bud13 PF09736 299 - 457 9.4e-40 Pre-mRNA-splicing factor of RES complex
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 785
AccII CGCG 1 cut(s) 367
AciI CCGC 6 cut(s) 5, 107, 365, 367, 554, 1021
AclWI GGATC 4 cut(s) 564, 860, 873, 1085
AcoI YGGCCR 1 cut(s) 799
AcuI CTGAAG 3 cut(s) 315, 1050, 1191
AcyI GRCGYC 1 cut(s) 274
AfeI AGCGCT 1 cut(s) 374
AfiI CCNNNNNNNGG 3 cut(s) 398, 785, 1190
AgeI ACCGGT 1 cut(s) 157
AgsI TTSAA 8 cut(s) 37, 476, 515, 645, 769, 857, 949, 1360
AjnI CCWGG 2 cut(s) 397, 1303
AluBI AGCT 8 cut(s) 167, 194, 839, 1028, 1034, 1039, 1254, 1382
AluI AGCT 8 cut(s) 167, 194, 839, 1028, 1034, 1039, 1254, 1382
Alw26I GTCTC 3 cut(s) 62, 244, 1303
AlwI GGATC 4 cut(s) 564, 860, 873, 1085
Aor51HI AGCGCT 1 cut(s) 374
AoxI GGCC 1 cut(s) 799
ApeKI GCWGC 2 cut(s) 890, 1277
AsiGI ACCGGT 1 cut(s) 157
Asp700I GAANNNNTTC 2 cut(s) 406, 1176
AspA2I CCTAGG 1 cut(s) 1189
AspLEI GCGC 1 cut(s) 375
AspS9I GGNCC 2 cut(s) 319, 761
AsuC2I CCSGG 1 cut(s) 759
AsuHPI GGTGA 9 cut(s) 172, 247, 351, 381, 443, 555, 602, 683, 1145
AvaII GGWCC 2 cut(s) 319, 761
AvrII CCTAGG 1 cut(s) 1189
AxyI CCTNAGG 2 cut(s) 978, 1233
BamHI GGATCC 1 cut(s) 865
BanII GRGCYC 1 cut(s) 271
BbsI GAAGAC 2 cut(s) 144, 231
BbvI GCAGC 2 cut(s) 902, 1264
BccI CCATC 5 cut(s) 293, 587, 856, 1122, 1313
BciT130I CCWGG 2 cut(s) 399, 1305
BciVI GTATCC 1 cut(s) 1176
BcnI CCSGG 1 cut(s) 759
BcoDI GTCTC 3 cut(s) 62, 244, 1303
BfaI CTAG 4 cut(s) 51, 1040, 1083, 1190
BfoI RGCGCY 1 cut(s) 376
BfuI GTATCC 1 cut(s) 1176
BglII AGATCT 7 cut(s) 439, 478, 517, 598, 637, 679, 721
BisI GCNGC 3 cut(s) 6, 891, 1278
BlnI CCTAGG 1 cut(s) 1189
BlpI GCTNAGC 2 cut(s) 840, 1035
BlsI GCNGC 3 cut(s) 7, 892, 1279
Bme1390I CCNGG 3 cut(s) 399, 759, 1305
Bme18I GGWCC 2 cut(s) 319, 761
BmgT120I GGNCC 2 cut(s) 319, 761
BmiI GGNNCC 4 cut(s) 321, 340, 762, 867
BmrFI CCNGG 3 cut(s) 399, 759, 1305
BmrI ACTGGG 1 cut(s) 888
BmsI GCATC 3 cut(s) 274, 664, 1264
BmuI ACTGGG 1 cut(s) 888
BpiI GAAGAC 2 cut(s) 144, 231
Bpu10I CCTNAGC 1 cut(s) 393
Bpu1102I GCTNAGC 2 cut(s) 840, 1035
BpuEI CTTGAG 1 cut(s) 275
BpuMI CCSGG 1 cut(s) 759
Bsa29I ATCGAT 1 cut(s) 1288
BsaBI GATNNNNATC 6 cut(s) 46, 438, 597, 678, 720, 949
BsaHI GRCGYC 1 cut(s) 274
BsaWI WCCGGW 1 cut(s) 157
BsaXI ACNNNNNCTCC 4 cut(s) 415, 445, 974, 1004
Bsc4I CCNNNNNNNGG 3 cut(s) 398, 785, 1190
Bse118I RCCGGY 1 cut(s) 157
Bse1I ACTGG 1 cut(s) 883
Bse21I CCTNAGG 2 cut(s) 978, 1233
Bse3DI GCAATG 1 cut(s) 120
Bse8I GATNNNNATC 6 cut(s) 46, 438, 597, 678, 720, 949
BseBI CCWGG 2 cut(s) 399, 1305
BseCI ATCGAT 1 cut(s) 1288
BseGI GGATG 2 cut(s) 249, 1324
BseJI GATNNNNATC 6 cut(s) 46, 438, 597, 678, 720, 949
BseLI CCNNNNNNNGG 3 cut(s) 398, 785, 1190
BseMI GCAATG 1 cut(s) 120
BseMII CTCAG 5 cut(s) 159, 384, 831, 1247, 1400
BseNI ACTGG 1 cut(s) 883
BseRI GAGGAG 4 cut(s) 218, 553, 950, 953
BseXI GCAGC 2 cut(s) 902, 1264
BsgI GTGCAG 1 cut(s) 1263
Bsh1236I CGCG 1 cut(s) 367
BshFI GGCC 1 cut(s) 801
BshTI ACCGGT 1 cut(s) 157
BshVI ATCGAT 1 cut(s) 1288
BsiSI CCGG 2 cut(s) 158, 758
BslFI GGGAC 2 cut(s) 261, 305
BslI CCNNNNNNNGG 3 cut(s) 398, 785, 1190
BsmAI GTCTC 3 cut(s) 62, 244, 1303
BsmBI CGTCTC 1 cut(s) 62
BsmFI GGGAC 2 cut(s) 261, 305
BsnI GGCC 1 cut(s) 801
Bsp1286I GDGCHC 1 cut(s) 271
Bsp1720I GCTNAGC 2 cut(s) 840, 1035
BspACI CCGC 6 cut(s) 5, 107, 365, 367, 554, 1021
BspANI GGCC 1 cut(s) 801
BspCNI CTCAG 5 cut(s) 160, 385, 832, 1246, 1399
BspDI ATCGAT 1 cut(s) 1288
BspFNI CGCG 1 cut(s) 367
BspLI GGNNCC 4 cut(s) 321, 340, 762, 867
BspPI GGATC 4 cut(s) 564, 860, 873, 1085
BspQI GCTCTTC 1 cut(s) 92
BsrDI GCAATG 1 cut(s) 120
BsrFI RCCGGY 1 cut(s) 157
BsrI ACTGG 1 cut(s) 883
BssAI RCCGGY 1 cut(s) 157
BssNI GRCGYC 1 cut(s) 274
BssT1I CCWWGG 5 cut(s) 611, 650, 692, 734, 1189
Bst2UI CCWGG 2 cut(s) 399, 1305
BstACI GRCGYC 1 cut(s) 274
BstC8I GCNNGC 1 cut(s) 1202
BstDEI CTNAG 7 cut(s) 168, 393, 840, 978, 1035, 1233, 1386
BstDSI CCRYGG 1 cut(s) 365
BstEII GGTNACC 1 cut(s) 1133
BstENI CCTNNNNNAGG 1 cut(s) 1188
BstF5I GGATG 2 cut(s) 249, 1324
BstFNI CGCG 1 cut(s) 367
BstH2I RGCGCY 1 cut(s) 376
BstHHI GCGC 1 cut(s) 375
BstMAI GTCTC 3 cut(s) 62, 244, 1303
BstNI CCWGG 2 cut(s) 399, 1305
BstNSI RCATGY 1 cut(s) 931
BstPI GGTNACC 1 cut(s) 1133
BstSCI CCNGG 3 cut(s) 397, 757, 1303
BstUI CGCG 1 cut(s) 367
BstV1I GCAGC 2 cut(s) 902, 1264
BstV2I GAAGAC 2 cut(s) 144, 231
BstX2I RGATCY 9 cut(s) 439, 478, 517, 556, 598, 637, 679, 721, 865
BstYI RGATCY 9 cut(s) 439, 478, 517, 556, 598, 637, 679, 721, 865
Bsu15I ATCGAT 1 cut(s) 1288
Bsu36I CCTNAGG 2 cut(s) 978, 1233
BsuI GTATCC 1 cut(s) 1176
BsuRI GGCC 1 cut(s) 801
BsuTUI ATCGAT 1 cut(s) 1288
BtgI CCRYGG 1 cut(s) 365
BtsCI GGATG 2 cut(s) 249, 1324
BtsI GCAGTG 1 cut(s) 310
BtsIMutI CAGTG 1 cut(s) 310
Cac8I GCNNGC 1 cut(s) 1202
CfoI GCGC 1 cut(s) 375
Cfr10I RCCGGY 1 cut(s) 157
Cfr13I GGNCC 2 cut(s) 319, 761
Cfr42I CCGCGG 1 cut(s) 368
ClaI ATCGAT 1 cut(s) 1288
CseI GACGC 1 cut(s) 263
CspAI ACCGGT 1 cut(s) 157
CviAII CATG 1 cut(s) 928
DdeI CTNAG 7 cut(s) 168, 393, 840, 978, 1035, 1233, 1386
EaeI YGGCCR 1 cut(s) 799
EciI GGCGGA 1 cut(s) 122
Eco130I CCWWGG 5 cut(s) 611, 650, 692, 734, 1189
Eco24I GRGCYC 1 cut(s) 271
Eco32I GATATC 2 cut(s) 795, 808
Eco47I GGWCC 2 cut(s) 319, 761
Eco47III AGCGCT 1 cut(s) 374
Eco57I CTGAAG 3 cut(s) 315, 1050, 1191
Eco81I CCTNAGG 2 cut(s) 978, 1233
Eco91I GGTNACC 1 cut(s) 1133
EcoNI CCTNNNNNAGG 1 cut(s) 1188
EcoO109I RGGNCCY 2 cut(s) 319, 761
EcoO65I GGTNACC 1 cut(s) 1133
EcoRII CCWGG 2 cut(s) 397, 1303
EcoRV GATATC 2 cut(s) 795, 808
EcoT14I CCWWGG 5 cut(s) 611, 650, 692, 734, 1189
EcoT38I GRGCYC 1 cut(s) 271
ErhI CCWWGG 5 cut(s) 611, 650, 692, 734, 1189
Esp3I CGTCTC 1 cut(s) 62
FaeI CATG 1 cut(s) 931
FaiI YATR 7 cut(s) 282, 290, 929, 1251, 1293, 1298, 1421
FaqI GGGAC 2 cut(s) 261, 305
FatI CATG 1 cut(s) 927
FauI CCCGC 1 cut(s) 1014
Fnu4HI GCNGC 3 cut(s) 6, 891, 1278
FokI GGATG 2 cut(s) 256, 1331
FriOI GRGCYC 1 cut(s) 271
Fsp4HI GCNGC 3 cut(s) 6, 891, 1278
FspBI CTAG 4 cut(s) 51, 1040, 1083, 1190
GlaI GCGC 1 cut(s) 374
GluI GCNGC 3 cut(s) 6, 891, 1278
HaeII RGCGCY 1 cut(s) 376
HaeIII GGCC 1 cut(s) 801
HapII CCGG 2 cut(s) 158, 758
HgaI GACGC 1 cut(s) 263
HhaI GCGC 1 cut(s) 375
Hin1I GRCGYC 1 cut(s) 274
Hin1II CATG 1 cut(s) 931
Hin6I GCGC 1 cut(s) 373
HinP1I GCGC 1 cut(s) 373
HinfI GANTC 3 cut(s) 47, 619, 1214
HpaII CCGG 2 cut(s) 158, 758
HphI GGTGA 9 cut(s) 172, 247, 351, 381, 443, 555, 602, 683, 1145
Hpy188I TCNGA 8 cut(s) 295, 327, 343, 624, 874, 934, 955, 1389
Hpy188III TCNNGA 5 cut(s) 849, 1094, 1169, 1218, 1235
HpyAV CCTTC 6 cut(s) 329, 654, 879, 1054, 1108, 1204
HpyCH4IV ACGT 1 cut(s) 1054
HpyCH4V TGCA 4 cut(s) 635, 677, 1076, 1280
HpyF3I CTNAG 7 cut(s) 168, 393, 840, 978, 1035, 1233, 1386
HpySE526I ACGT 1 cut(s) 1054
Hsp92I GRCGYC 1 cut(s) 274
Hsp92II CATG 1 cut(s) 931
HspAI GCGC 1 cut(s) 373
KspI CCGCGG 1 cut(s) 368
LguI GCTCTTC 1 cut(s) 92
LmnI GCTCC 2 cut(s) 344, 940
Lsp1109I GCAGC 2 cut(s) 902, 1264
LweI GCATC 3 cut(s) 274, 664, 1264
MaeI CTAG 4 cut(s) 51, 1040, 1083, 1190
MaeII ACGT 1 cut(s) 1054
MaeIII GTNAC 3 cut(s) 357, 1133, 1334
MflI RGATCY 9 cut(s) 439, 478, 517, 556, 598, 637, 679, 721, 865
MhlI GDGCHC 1 cut(s) 271
MluCI AATT 1 cut(s) 187
MlyI GAGTC 1 cut(s) 628
MmeI TCCRAC 3 cut(s) 602, 912, 972
MroXI GAANNNNTTC 2 cut(s) 406, 1176
MseI TTAA 5 cut(s) 24, 123, 263, 315, 1259
MslI CAYNNNNRTG 2 cut(s) 233, 1317
MspA1I CMGCKG 2 cut(s) 194, 367
MspI CCGG 2 cut(s) 158, 758
MspR9I CCNGG 3 cut(s) 399, 759, 1305
MvaI CCWGG 2 cut(s) 399, 1305
MvnI CGCG 1 cut(s) 367
NciI CCSGG 1 cut(s) 759
NlaIII CATG 1 cut(s) 931
NlaIV GGNNCC 4 cut(s) 321, 340, 762, 867
NmeAIII GCCGAG 2 cut(s) 250, 827
NmuCI GTSAC 2 cut(s) 357, 1133
NspI RCATGY 1 cut(s) 931
PciSI GCTCTTC 1 cut(s) 92
PdmI GAANNNNTTC 2 cut(s) 406, 1176
PfeI GAWTC 2 cut(s) 47, 1214
PflMI CCANNNNNTGG 1 cut(s) 785
PinAI ACCGGT 1 cut(s) 157
PkrI GCNGC 3 cut(s) 7, 892, 1279
PleI GAGTC 1 cut(s) 627
PpsI GAGTC 1 cut(s) 627
PpuMI RGGWCCY 2 cut(s) 319, 761
Psp5II RGGWCCY 2 cut(s) 319, 761
Psp6I CCWGG 2 cut(s) 397, 1303
PspEI GGTNACC 1 cut(s) 1133
PspGI CCWGG 2 cut(s) 397, 1303
PspN4I GGNNCC 4 cut(s) 321, 340, 762, 867
PspPI GGNCC 2 cut(s) 319, 761
PspPPI RGGWCCY 2 cut(s) 319, 761
PsuI RGATCY 9 cut(s) 439, 478, 517, 556, 598, 637, 679, 721, 865
PvuII CAGCTG 1 cut(s) 194
RseI CAYNNNNRTG 2 cut(s) 233, 1317
SacII CCGCGG 1 cut(s) 368
SapI GCTCTTC 1 cut(s) 92
SaqAI TTAA 5 cut(s) 24, 123, 263, 315, 1259
SatI GCNGC 3 cut(s) 6, 891, 1278
Sau96I GGNCC 2 cut(s) 319, 761
SchI GAGTC 1 cut(s) 628
ScrFI CCNGG 3 cut(s) 399, 759, 1305
SduI GDGCHC 1 cut(s) 271
SfaNI GCATC 3 cut(s) 274, 664, 1264
Sfr303I CCGCGG 1 cut(s) 368
SgrBI CCGCGG 1 cut(s) 368
SinI GGWCC 2 cut(s) 319, 761
SmiMI CAYNNNNRTG 2 cut(s) 233, 1317
SmlI CTYRAG 1 cut(s) 254
SmoI CTYRAG 1 cut(s) 254
Sse9I AATT 1 cut(s) 187
SsiI CCGC 6 cut(s) 5, 107, 365, 367, 554, 1021
SspMI CTAG 4 cut(s) 51, 1040, 1083, 1190
StyD4I CCNGG 3 cut(s) 397, 757, 1303
StyI CCWWGG 5 cut(s) 611, 650, 692, 734, 1189
TaiI ACGT 1 cut(s) 1057
TaqI TCGA 3 cut(s) 410, 848, 1288
TasI AATT 1 cut(s) 187
TauI GCSGC 1 cut(s) 8
TfiI GAWTC 2 cut(s) 47, 1214
Tru1I TTAA 5 cut(s) 24, 123, 263, 315, 1259
Tru9I TTAA 5 cut(s) 24, 123, 263, 315, 1259
TscAI CASTG 1 cut(s) 310
TseFI GTSAC 2 cut(s) 357, 1133
TseI GCWGC 2 cut(s) 890, 1277
Tsp45I GTSAC 2 cut(s) 357, 1133
TspDTI ATGAA 9 cut(s) 150, 237, 260, 368, 450, 732, 984, 1127, 1223
TspGWI ACGGA 4 cut(s) 1093, 1353, 1378, 1406
TspRI CASTG 1 cut(s) 310
Van91I CCANNNNNTGG 1 cut(s) 785
VpaK11BI GGWCC 2 cut(s) 319, 761
XagI CCTNNNNNAGG 1 cut(s) 1188
XceI RCATGY 1 cut(s) 931
XcmI CCANNNNNNNNNTGG 1 cut(s) 1311
XmaJI CCTAGG 1 cut(s) 1189
XmnI GAANNNNTTC 2 cut(s) 406, 1176
XspI CTAG 4 cut(s) 51, 1040, 1083, 1190
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.