MD05G1301400.v1.1

Belongs to the 14-3-3 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0004001
Physical Location & Seq
Reverse (-)
43423732 .. 43426889
3158 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1301400.v1.1.491

Sequence Viewer

Length: 786 bp
ATGGCTTCCCCCAAGGAGAGAGAGAACTACGTCTACACTGCTAAGCTCGCCGAGCAAGCCGAGCGTTACGACGAAATGGTGGAGGCGATGGCGAAAGTAGCGAAGCTTGACGTGGAGCTCACCGTAGAGGAGCGGAATCTGCTTTCGGTTGGGTACAAGAACGTGATCGGAGCTCGCAGGGCTTCGTGGAGAATTCTGTCCTCGATTGAGCAGAAGGAGGAGGGGAAAGGGAACGATCAGAATGTGAGCCGTATCAAGGAGTACAGGCAAAAGGTTGAGTCCGAGCTCTCCAGCATTTGCAGCGACATCATGAGGGTTATCGATGAGCATCTCATTCCCTCGTGCACCGCTTTTGAGTCCACTGTGTTTTTCTACAAAATGAAAGGAGATTACTATCGCTACTTGGCGGAGTTTAAGACTGGTGATGATAGGAAAGAGGTTGCTGATCAGTCAATGAAAGCTTATCAGGCAGCTTCTAGTAAGGCAGAGAGTGACTTACCCCCCACACACCCCATCAGACTCGGTTTGGCCTTGAACTTCTCTGTCTTTTACTATGAAATATTGAACTCTCCGGAAAGGGCTTGTCACCTTGCAAAGCAAGCTTTTGATGAAGCTATCTCAGAATTAGATACTTTGAGCGAGGAATCGTACAAGGATAGCACCTTGATCATGCAGCTTTTAAGGGACAACCTTACTTTGTGGACCTCTGACATTCCAGAGGATGGAGCCGAGGATATCCAGAAGGTGGATGGCACTGCTAAACCTGGAGGTGAAGATGCAGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04011 ko04110 ko04114 ko04151 ko04390 ko04391 ko04722 ko05169 ko05203 map04011 map04110 map04114 map04151 map04390 map04391 map04722 map05169 map05203
Pfam Domains
Protein Families
14-3-3

Protein Analysis

262

Amino Acids

29.62

Weight (kDa)

4.77

Isoelectric Point (pI)

44.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
14-3-3 PF00244 13 - 235 1.7e-108 14-3-3 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 722, 745
AccBSI CCGCTC 1 cut(s) 133
AccI GTMKAC 1 cut(s) 33
AccIII TCCGGA 1 cut(s) 571
AciI CCGC 3 cut(s) 133, 348, 407
AcsI RAATTY 1 cut(s) 192
AfaI GTAC 3 cut(s) 155, 263, 650
AfiI CCNNNNNNNGG 3 cut(s) 256, 722, 745
AgsI TTSAA 2 cut(s) 535, 565
AjiI CACGTC 1 cut(s) 112
AjnI CCWGG 1 cut(s) 763
Alw21I GWGCWC 4 cut(s) 120, 175, 288, 347
Alw44I GTGCAC 1 cut(s) 343
Aor13HI TCCGGA 1 cut(s) 571
AoxI GGCC 1 cut(s) 528
ApaLI GTGCAC 1 cut(s) 343
ApeKI GCWGC 3 cut(s) 300, 470, 673
ApoI RAATTY 1 cut(s) 192
AspS9I GGNCC 1 cut(s) 702
AsuHPI GGTGA 4 cut(s) 112, 434, 578, 782
AvaII GGWCC 1 cut(s) 702
BaeGI GKGCMC 1 cut(s) 347
BanII GRGCYC 3 cut(s) 120, 175, 288
BauI CACGAG 1 cut(s) 340
Bbv12I GWGCWC 4 cut(s) 120, 175, 288, 347
BbvI GCAGC 3 cut(s) 312, 482, 685
BccI CCATC 4 cut(s) 82, 521, 716, 743
BceAI ACGGC 1 cut(s) 234
BciT130I CCWGG 1 cut(s) 765
BclI TGATCA 2 cut(s) 445, 666
BfaI CTAG 1 cut(s) 477
BisI GCNGC 3 cut(s) 301, 471, 674
BlpI GCTNAGC 1 cut(s) 42
BlsI GCNGC 3 cut(s) 302, 472, 675
Bme1390I CCNGG 1 cut(s) 765
Bme18I GGWCC 1 cut(s) 702
BmgBI CACGTC 1 cut(s) 112
BmgT120I GGNCC 1 cut(s) 702
BmiI GGNNCC 1 cut(s) 727
BmrFI CCNGG 1 cut(s) 765
BmsI GCATC 2 cut(s) 337, 766
BpmI CTGGAG 2 cut(s) 274, 786
Bpu1102I GCTNAGC 1 cut(s) 42
Bsa29I ATCGAT 1 cut(s) 321
BsaBI GATNNNNATC 2 cut(s) 327, 393
BsaJI CCNNGG 2 cut(s) 12, 729
BsaWI WCCGGW 1 cut(s) 571
Bsc4I CCNNNNNNNGG 3 cut(s) 256, 722, 745
Bse1I ACTGG 1 cut(s) 424
Bse8I GATNNNNATC 2 cut(s) 327, 393
BseAI TCCGGA 1 cut(s) 571
BseBI CCWGG 1 cut(s) 765
BseCI ATCGAT 1 cut(s) 321
BseDI CCNNGG 2 cut(s) 12, 729
BseGI GGATG 2 cut(s) 727, 754
BseJI GATNNNNATC 2 cut(s) 327, 393
BseLI CCNNNNNNNGG 3 cut(s) 256, 722, 745
BseMII CTCAG 1 cut(s) 633
BseNI ACTGG 1 cut(s) 424
BseRI GAGGAG 2 cut(s) 143, 233
BseSI GKGCMC 1 cut(s) 347
BseXI GCAGC 3 cut(s) 312, 482, 685
BshFI GGCC 1 cut(s) 530
BshVI ATCGAT 1 cut(s) 321
BsiHKAI GWGCWC 4 cut(s) 120, 175, 288, 347
BsiSI CCGG 1 cut(s) 572
BslFI GGGAC 1 cut(s) 698
BslI CCNNNNNNNGG 3 cut(s) 256, 722, 745
BsmFI GGGAC 1 cut(s) 698
BsnI GGCC 1 cut(s) 530
Bsp1286I GDGCHC 4 cut(s) 120, 175, 288, 347
Bsp13I TCCGGA 1 cut(s) 571
Bsp143I GATC 4 cut(s) 165, 235, 445, 666
Bsp1720I GCTNAGC 1 cut(s) 42
BspACI CCGC 3 cut(s) 133, 348, 407
BspANI GGCC 1 cut(s) 530
BspCNI CTCAG 1 cut(s) 632
BspDI ATCGAT 1 cut(s) 321
BspEI TCCGGA 1 cut(s) 571
BspHI TCATGA 1 cut(s) 309
BspLI GGNNCC 1 cut(s) 727
BsrBI CCGCTC 1 cut(s) 133
BsrI ACTGG 1 cut(s) 424
BssECI CCNNGG 2 cut(s) 12, 729
BssMI GATC 4 cut(s) 165, 235, 445, 666
BssSI CACGAG 1 cut(s) 340
BssT1I CCWWGG 1 cut(s) 12
Bst2BI CACGAG 1 cut(s) 340
Bst2UI CCWGG 1 cut(s) 765
Bst4CI ACNGT 2 cut(s) 124, 364
BstC8I GCNNGC 4 cut(s) 48, 57, 175, 600
BstDEI CTNAG 2 cut(s) 42, 619
BstF5I GGATG 2 cut(s) 727, 754
BstKTI GATC 4 cut(s) 168, 238, 448, 669
BstMBI GATC 4 cut(s) 165, 235, 445, 666
BstNI CCWGG 1 cut(s) 765
BstSCI CCNGG 1 cut(s) 763
BstSLI GKGCMC 1 cut(s) 347
BstV1I GCAGC 3 cut(s) 312, 482, 685
Bsu15I ATCGAT 1 cut(s) 321
BsuRI GGCC 1 cut(s) 530
BsuTUI ATCGAT 1 cut(s) 321
BtgZI GCGATG 1 cut(s) 101
BtrI CACGTC 1 cut(s) 112
BtsCI GGATG 2 cut(s) 727, 754
BtsI GCAGTG 2 cut(s) 36, 753
BtsIMutI CAGTG 3 cut(s) 36, 360, 753
Cac8I GCNNGC 4 cut(s) 48, 57, 175, 600
CciI TCATGA 1 cut(s) 309
Cfr13I GGNCC 1 cut(s) 702
ClaI ATCGAT 1 cut(s) 321
Csp6I GTAC 3 cut(s) 154, 262, 649
CviAII CATG 2 cut(s) 310, 670
CviQI GTAC 3 cut(s) 154, 262, 649
DdeI CTNAG 2 cut(s) 42, 619
DpnI GATC 4 cut(s) 167, 237, 447, 668
DpnII GATC 4 cut(s) 165, 235, 445, 666
EciI GGCGGA 1 cut(s) 422
Ecl136II GAGCTC 3 cut(s) 118, 173, 286
Eco130I CCWWGG 1 cut(s) 12
Eco24I GRGCYC 3 cut(s) 120, 175, 288
Eco32I GATATC 1 cut(s) 736
Eco47I GGWCC 1 cut(s) 702
Eco53kI GAGCTC 3 cut(s) 118, 173, 286
EcoICRI GAGCTC 3 cut(s) 118, 173, 286
EcoRI GAATTC 1 cut(s) 192
EcoRII CCWGG 1 cut(s) 763
EcoRV GATATC 1 cut(s) 736
EcoT14I CCWWGG 1 cut(s) 12
EcoT38I GRGCYC 3 cut(s) 120, 175, 288
ErhI CCWWGG 1 cut(s) 12
FaeI CATG 2 cut(s) 313, 673
FaiI YATR 3 cut(s) 311, 555, 671
FaqI GGGAC 1 cut(s) 698
FatI CATG 2 cut(s) 309, 669
FbaI TGATCA 2 cut(s) 445, 666
FblI GTMKAC 1 cut(s) 33
Fnu4HI GCNGC 3 cut(s) 301, 471, 674
FokI GGATG 2 cut(s) 734, 761
FriOI GRGCYC 3 cut(s) 120, 175, 288
Fsp4HI GCNGC 3 cut(s) 301, 471, 674
FspBI CTAG 1 cut(s) 477
GluI GCNGC 3 cut(s) 301, 471, 674
GsuI CTGGAG 2 cut(s) 274, 786
HaeIII GGCC 1 cut(s) 530
HapII CCGG 1 cut(s) 572
Hin1II CATG 2 cut(s) 313, 673
HindIII AAGCTT 3 cut(s) 104, 459, 600
HinfI GANTC 5 cut(s) 136, 278, 356, 519, 644
HpaII CCGG 1 cut(s) 572
HphI GGTGA 4 cut(s) 112, 434, 578, 782
Hpy166II GTNNAC 4 cut(s) 34, 345, 360, 702
Hpy188I TCNGA 6 cut(s) 170, 240, 283, 518, 622, 709
Hpy188III TCNNGA 4 cut(s) 310, 572, 716, 739
Hpy8I GTNNAC 4 cut(s) 34, 345, 360, 702
Hpy99I CGWCG 1 cut(s) 74
HpyAV CCTTC 2 cut(s) 208, 736
HpyCH4III ACNGT 2 cut(s) 124, 364
HpyCH4IV ACGT 3 cut(s) 30, 111, 162
HpyCH4V TGCA 5 cut(s) 300, 345, 593, 673, 779
HpyF3I CTNAG 2 cut(s) 42, 619
HpySE526I ACGT 3 cut(s) 30, 111, 162
Hsp92II CATG 2 cut(s) 313, 673
Kpn2I TCCGGA 1 cut(s) 571
Ksp22I TGATCA 2 cut(s) 445, 666
Kzo9I GATC 4 cut(s) 165, 235, 445, 666
LmnI GCTCC 4 cut(s) 115, 130, 170, 725
Lsp1109I GCAGC 3 cut(s) 312, 482, 685
LweI GCATC 2 cut(s) 337, 766
MaeI CTAG 1 cut(s) 477
MaeII ACGT 3 cut(s) 30, 111, 162
MaeIII GTNAC 3 cut(s) 65, 491, 584
MalI GATC 4 cut(s) 167, 237, 447, 668
MbiI CCGCTC 1 cut(s) 133
MboI GATC 4 cut(s) 165, 235, 445, 666
MboII GAAGA 1 cut(s) 785
MhlI GDGCHC 4 cut(s) 120, 175, 288, 347
MluCI AATT 2 cut(s) 192, 623
MlyI GAGTC 3 cut(s) 287, 365, 513
MroI TCCGGA 1 cut(s) 571
MseI TTAA 2 cut(s) 414, 680
MspI CCGG 1 cut(s) 572
MspR9I CCNGG 1 cut(s) 765
MvaI CCWGG 1 cut(s) 765
NdeII GATC 4 cut(s) 165, 235, 445, 666
NlaIII CATG 2 cut(s) 313, 673
NlaIV GGNNCC 1 cut(s) 727
NmeAIII GCCGAG 3 cut(s) 76, 85, 754
NmuCI GTSAC 2 cut(s) 491, 584
PagI TCATGA 1 cut(s) 309
PfeI GAWTC 2 cut(s) 136, 644
PflMI CCANNNNNTGG 2 cut(s) 722, 745
PkrI GCNGC 3 cut(s) 302, 472, 675
PleI GAGTC 3 cut(s) 286, 364, 513
PpsI GAGTC 3 cut(s) 286, 364, 513
Psp124BI GAGCTC 3 cut(s) 120, 175, 288
Psp6I CCWGG 1 cut(s) 763
PspGI CCWGG 1 cut(s) 763
PspN4I GGNNCC 1 cut(s) 727
PspPI GGNCC 1 cut(s) 702
PsrI GAACNNNNNNTAC 2 cut(s) 17, 49
RsaI GTAC 3 cut(s) 155, 263, 650
RsaNI GTAC 3 cut(s) 154, 262, 649
SacI GAGCTC 3 cut(s) 120, 175, 288
SaqAI TTAA 2 cut(s) 414, 680
SatI GCNGC 3 cut(s) 301, 471, 674
Sau3AI GATC 4 cut(s) 165, 235, 445, 666
Sau96I GGNCC 1 cut(s) 702
SchI GAGTC 3 cut(s) 287, 365, 513
ScrFI CCNGG 1 cut(s) 765
SduI GDGCHC 4 cut(s) 120, 175, 288, 347
SfaNI GCATC 2 cut(s) 337, 766
SinI GGWCC 1 cut(s) 702
Sse9I AATT 2 cut(s) 192, 623
SsiI CCGC 3 cut(s) 133, 348, 407
SspI AATATT 1 cut(s) 561
SspMI CTAG 1 cut(s) 477
SstI GAGCTC 3 cut(s) 120, 175, 288
StyD4I CCNGG 1 cut(s) 763
StyI CCWWGG 1 cut(s) 12
TaaI ACNGT 2 cut(s) 124, 364
TaiI ACGT 3 cut(s) 33, 114, 165
TaqI TCGA 2 cut(s) 203, 321
TasI AATT 2 cut(s) 192, 623
TatI WGTACW 1 cut(s) 261
TfiI GAWTC 2 cut(s) 136, 644
Tru1I TTAA 2 cut(s) 414, 680
Tru9I TTAA 2 cut(s) 414, 680
TscAI CASTG 3 cut(s) 43, 367, 760
TseFI GTSAC 2 cut(s) 491, 584
TseI GCWGC 3 cut(s) 300, 470, 673
Tsp45I GTSAC 2 cut(s) 491, 584
TspDTI ATGAA 4 cut(s) 395, 470, 570, 624
TspRI CASTG 3 cut(s) 43, 367, 760
Van91I CCANNNNNTGG 2 cut(s) 722, 745
VneI GTGCAC 1 cut(s) 343
VpaK11BI GGWCC 1 cut(s) 702
XapI RAATTY 1 cut(s) 192
XcmI CCANNNNNNNNNTGG 1 cut(s) 746
XmiI GTMKAC 1 cut(s) 33
XspI CTAG 1 cut(s) 477
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.