MD17G1231000.v1.1

Belongs to the 14-3-3 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0004001
Physical Location & Seq
Forward (+)
27941075 .. 27945515
4441 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1231000.v1.1.491

Sequence Viewer

Length: 783 bp
ATGGGTTTCGCTACTGAACGTGAGAACTTCGTCTACCTTGCCAAGCTCGCTGAGCAGGCAGAGCGATACGATGAGATGGTCGATGCAATGAAGAAGGTAGCAAATCTTGATGTTGAATTGACAGTTGAAGAGAGAAACTTACTTTCTGTTGGGTACAAGAATGTTGTTGGTGCTCGTCGGGCATCATGGAGGATCCTATCATCAATAGAACAGAAAGAGGAATCAAGAGGAAATGAGAGTCATGTGAAGCAACTCAAGGAGTATAGACAGAAGGTTGAATCAGAGCTCTCAACCATTTGTGTTGATATCATGGCAGTGATCGATGAGCATCTCATTCCTTCAGCTACAGTTGGTGAATCAACTGTTTTCTATTATAAGATGAAAGGAGATTATTATAGGTACCTTGCAGAATTCAAGAGTGGTGACGATAAAACAGAGGCTGCTGAACAGTCGAAGAAAGCATACGAGACTGCCACCACCACTGCTGAGGCCGAGTTGCCTTCTACACATCCCATCCGTTTGGGTCTGGCTCTGAATTTCTCAGTCTTCTATTATGAGATCATGAATTCTCCAGAAAGGGCTTGCCACCTTGCAAAGACAGCTTTTGATGAAGCTATTGCTGAGCTTGACTCTTTGAATGAGGAGTCATACAAGGATAGCACCTTAATCATGCAGCTTCTAAGGGACAACCTCACGTTGTGGACTTCTGACCTTCCAGAAGATGGAGATTCCCAAAAGGTGAATGGCAATGCCAAGGTTGGCGGGCTGGAAGAGGCAACCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04011 ko04110 ko04114 ko04151 ko04390 ko04391 ko04722 ko05169 ko05203 map04011 map04110 map04114 map04151 map04390 map04391 map04722 map05169 map05203
Pfam Domains
Protein Families
14-3-3

Protein Analysis

261

Amino Acids

29.36

Weight (kDa)

4.75

Isoelectric Point (pI)

41.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
14-3-3 PF00244 13 - 235 3.4e-108 14-3-3 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 375
Acc65I GGTACC 1 cut(s) 399
AccB1I GGYRCC 1 cut(s) 399
AccB7I CCANNNNNTGG 1 cut(s) 722
AccI GTMKAC 1 cut(s) 33
AciI CCGC 1 cut(s) 762
AclWI GGATC 2 cut(s) 187, 200
AcsI RAATTY 3 cut(s) 410, 535, 565
AcuI CTGAAG 1 cut(s) 324
AdeI CACNNNGTG 1 cut(s) 699
AfaI GTAC 2 cut(s) 155, 401
AfiI CCNNNNNNNGG 1 cut(s) 722
AgsI TTSAA 5 cut(s) 116, 128, 278, 415, 637
AluBI AGCT 7 cut(s) 46, 286, 344, 602, 614, 625, 676
AluI AGCT 7 cut(s) 46, 286, 344, 602, 614, 625, 676
Alw21I GWGCWC 2 cut(s) 175, 288
Alw26I GTCTC 1 cut(s) 461
AlwI GGATC 2 cut(s) 187, 200
AlwNI CAGNNNCTG 1 cut(s) 440
AoxI GGCC 1 cut(s) 489
ApeKI GCWGC 2 cut(s) 440, 673
ApoI RAATTY 3 cut(s) 410, 535, 565
Asp718I GGTACC 1 cut(s) 399
AsuHPI GGTGA 3 cut(s) 365, 434, 751
BamHI GGATCC 1 cut(s) 192
BanI GGYRCC 1 cut(s) 399
BanII GRGCYC 1 cut(s) 288
BarI GAAGNNNNNNTAC 2 cut(s) 446, 478
BbsI GAAGAC 1 cut(s) 538
Bbv12I GWGCWC 2 cut(s) 175, 288
BbvCI CCTCAGC 1 cut(s) 486
BbvI GCAGC 2 cut(s) 427, 685
BccI CCATC 3 cut(s) 70, 521, 716
BcoDI GTCTC 1 cut(s) 461
BfmI CTRYAG 1 cut(s) 345
BisI GCNGC 2 cut(s) 441, 674
BlpI GCTNAGC 2 cut(s) 51, 621
BlsI GCNGC 2 cut(s) 442, 675
BmiI GGNNCC 2 cut(s) 194, 401
BmsI GCATC 3 cut(s) 73, 191, 337
BpiI GAAGAC 1 cut(s) 538
BplI GAGNNNNNCTC 2 cut(s) 614, 646
BpmI CTGGAG 1 cut(s) 555
Bpu10I CCTNAGC 1 cut(s) 486
Bpu1102I GCTNAGC 2 cut(s) 51, 621
BpuEI CTTGAG 1 cut(s) 239
Bsa29I ATCGAT 1 cut(s) 321
BsaBI GATNNNNATC 1 cut(s) 327
BsaJI CCNNGG 1 cut(s) 753
Bsc4I CCNNNNNNNGG 1 cut(s) 722
Bse3DI GCAATG 2 cut(s) 93, 754
Bse8I GATNNNNATC 1 cut(s) 327
BseCI ATCGAT 1 cut(s) 321
BseDI CCNNGG 1 cut(s) 753
BseGI GGATG 2 cut(s) 508, 513
BseJI GATNNNNATC 1 cut(s) 327
BseLI CCNNNNNNNGG 1 cut(s) 722
BseMI GCAATG 2 cut(s) 93, 754
BseMII CTCAG 4 cut(s) 42, 477, 555, 612
BseRI GAGGAG 1 cut(s) 656
BseXI GCAGC 2 cut(s) 427, 685
BshFI GGCC 1 cut(s) 491
BshNI GGYRCC 1 cut(s) 399
BshVI ATCGAT 1 cut(s) 321
BsiHKAI GWGCWC 2 cut(s) 175, 288
BslFI GGGAC 1 cut(s) 698
BslI CCNNNNNNNGG 1 cut(s) 722
BsmAI GTCTC 1 cut(s) 461
BsmFI GGGAC 1 cut(s) 698
BsnI GGCC 1 cut(s) 491
Bsp1286I GDGCHC 2 cut(s) 175, 288
Bsp143I GATC 3 cut(s) 192, 318, 558
Bsp1720I GCTNAGC 2 cut(s) 51, 621
BspACI CCGC 1 cut(s) 762
BspANI GGCC 1 cut(s) 491
BspCNI CTCAG 4 cut(s) 43, 478, 554, 613
BspDI ATCGAT 1 cut(s) 321
BspHI TCATGA 1 cut(s) 561
BspLI GGNNCC 2 cut(s) 194, 401
BspPI GGATC 2 cut(s) 187, 200
BspT107I GGYRCC 1 cut(s) 399
BsrDI GCAATG 2 cut(s) 93, 754
BssECI CCNNGG 1 cut(s) 753
BssMI GATC 3 cut(s) 192, 318, 558
BssT1I CCWWGG 1 cut(s) 753
Bst4CI ACNGT 4 cut(s) 124, 349, 364, 450
Bst6I CTCTTC 2 cut(s) 123, 765
BstC8I GCNNGC 4 cut(s) 48, 57, 583, 764
BstDEI CTNAG 5 cut(s) 51, 486, 541, 621, 680
BstF5I GGATG 2 cut(s) 508, 513
BstKTI GATC 3 cut(s) 195, 321, 561
BstMAI GTCTC 1 cut(s) 461
BstMBI GATC 3 cut(s) 192, 318, 558
BstMWI GCNNNNNNNGC 6 cut(s) 47, 52, 56, 61, 179, 599
BstSFI CTRYAG 1 cut(s) 345
BstV1I GCAGC 2 cut(s) 427, 685
BstV2I GAAGAC 1 cut(s) 538
BstX2I RGATCY 1 cut(s) 192
BstXI CCANNNNNNTGG 1 cut(s) 520
BstYI RGATCY 1 cut(s) 192
Bsu15I ATCGAT 1 cut(s) 321
BsuRI GGCC 1 cut(s) 491
BsuTUI ATCGAT 1 cut(s) 321
BtsCI GGATG 2 cut(s) 508, 513
BtsI GCAGTG 2 cut(s) 321, 480
BtsIMutI CAGTG 2 cut(s) 321, 480
Cac8I GCNNGC 4 cut(s) 48, 57, 583, 764
CaiI CAGNNNCTG 1 cut(s) 440
CciI TCATGA 1 cut(s) 561
ClaI ATCGAT 1 cut(s) 321
Csp6I GTAC 2 cut(s) 154, 400
CviAII CATG 5 cut(s) 186, 242, 310, 562, 670
CviQI GTAC 2 cut(s) 154, 400
DdeI CTNAG 5 cut(s) 51, 486, 541, 621, 680
DpnI GATC 3 cut(s) 194, 320, 560
DpnII GATC 3 cut(s) 192, 318, 558
DraIII CACNNNGTG 1 cut(s) 699
Eam1104I CTCTTC 2 cut(s) 123, 765
EarI CTCTTC 2 cut(s) 123, 765
Ecl136II GAGCTC 1 cut(s) 286
Eco130I CCWWGG 1 cut(s) 753
Eco24I GRGCYC 1 cut(s) 288
Eco32I GATATC 1 cut(s) 307
Eco53kI GAGCTC 1 cut(s) 286
Eco57I CTGAAG 1 cut(s) 324
EcoICRI GAGCTC 1 cut(s) 286
EcoRI GAATTC 2 cut(s) 410, 565
EcoRV GATATC 1 cut(s) 307
EcoT14I CCWWGG 1 cut(s) 753
EcoT38I GRGCYC 1 cut(s) 288
ErhI CCWWGG 1 cut(s) 753
FaeI CATG 5 cut(s) 189, 245, 313, 565, 673
FaqI GGGAC 1 cut(s) 698
FatI CATG 5 cut(s) 185, 241, 309, 561, 669
FauI CCCGC 1 cut(s) 755
FblI GTMKAC 1 cut(s) 33
Fnu4HI GCNGC 2 cut(s) 441, 674
FokI GGATG 2 cut(s) 495, 500
FriOI GRGCYC 1 cut(s) 288
Fsp4HI GCNGC 2 cut(s) 441, 674
GluI GCNGC 2 cut(s) 441, 674
GsuI CTGGAG 1 cut(s) 555
HaeIII GGCC 1 cut(s) 491
Hin1II CATG 5 cut(s) 189, 245, 313, 565, 673
HinfI GANTC 7 cut(s) 221, 238, 278, 356, 629, 644, 728
HphI GGTGA 3 cut(s) 365, 434, 751
Hpy166II GTNNAC 2 cut(s) 34, 702
Hpy188I TCNGA 3 cut(s) 283, 534, 709
Hpy188III TCNNGA 6 cut(s) 107, 225, 415, 562, 572, 716
Hpy8I GTNNAC 2 cut(s) 34, 702
Hpy99I CGWCG 1 cut(s) 180
HpyAV CCTTC 5 cut(s) 88, 265, 348, 510, 722
HpyCH4III ACNGT 4 cut(s) 124, 349, 364, 450
HpyCH4IV ACGT 2 cut(s) 19, 695
HpyCH4V TGCA 4 cut(s) 86, 407, 593, 673
HpyF10VI GCNNNNNNNGC 6 cut(s) 47, 52, 56, 61, 179, 599
HpyF3I CTNAG 5 cut(s) 51, 486, 541, 621, 680
HpySE526I ACGT 2 cut(s) 19, 695
Hsp92II CATG 5 cut(s) 189, 245, 313, 565, 673
KpnI GGTACC 1 cut(s) 403
Kzo9I GATC 3 cut(s) 192, 318, 558
LpnPI CCDG 5 cut(s) 41, 512, 585, 729, 752
Lsp1109I GCAGC 2 cut(s) 427, 685
LweI GCATC 3 cut(s) 73, 191, 337
MaeII ACGT 2 cut(s) 19, 695
MaeIII GTNAC 1 cut(s) 422
MalI GATC 3 cut(s) 194, 320, 560
MboI GATC 3 cut(s) 192, 318, 558
MboII GAAGA 6 cut(s) 103, 140, 466, 538, 731, 782
MflI RGATCY 1 cut(s) 192
MhlI GDGCHC 2 cut(s) 175, 288
MluCI AATT 4 cut(s) 116, 410, 535, 565
MlyI GAGTC 3 cut(s) 247, 623, 653
MnlI CCTC 8 cut(s) 183, 211, 221, 430, 481, 634, 701, 766
MseI TTAA 1 cut(s) 665
MslI CAYNNNNRTG 1 cut(s) 314
MwoI GCNNNNNNNGC 6 cut(s) 47, 52, 56, 61, 179, 599
NdeII GATC 3 cut(s) 192, 318, 558
NlaIII CATG 5 cut(s) 189, 245, 313, 565, 673
NlaIV GGNNCC 2 cut(s) 194, 401
NmeAIII GCCGAG 1 cut(s) 517
NmuCI GTSAC 1 cut(s) 422
PagI TCATGA 1 cut(s) 561
PfeI GAWTC 4 cut(s) 221, 278, 356, 728
PflMI CCANNNNNTGG 1 cut(s) 722
PkrI GCNGC 2 cut(s) 442, 675
PleI GAGTC 3 cut(s) 246, 623, 652
PpsI GAGTC 3 cut(s) 246, 623, 652
PsiI TTATAA 1 cut(s) 375
Psp124BI GAGCTC 1 cut(s) 288
PspN4I GGNNCC 2 cut(s) 194, 401
PsrI GAACNNNNNNTAC 2 cut(s) 17, 49
PstNI CAGNNNCTG 1 cut(s) 440
PsuI RGATCY 1 cut(s) 192
RsaI GTAC 2 cut(s) 155, 401
RsaNI GTAC 2 cut(s) 154, 400
RseI CAYNNNNRTG 1 cut(s) 314
SacI GAGCTC 1 cut(s) 288
SaqAI TTAA 1 cut(s) 665
SatI GCNGC 2 cut(s) 441, 674
Sau3AI GATC 3 cut(s) 192, 318, 558
SchI GAGTC 3 cut(s) 247, 623, 653
SduI GDGCHC 2 cut(s) 175, 288
SfaNI GCATC 3 cut(s) 73, 191, 337
SfcI CTRYAG 1 cut(s) 345
SmiMI CAYNNNNRTG 1 cut(s) 314
SmlI CTYRAG 1 cut(s) 254
SmoI CTYRAG 1 cut(s) 254
Sse9I AATT 4 cut(s) 116, 410, 535, 565
SsiI CCGC 1 cut(s) 762
SstI GAGCTC 1 cut(s) 288
StyI CCWWGG 1 cut(s) 753
TaaI ACNGT 4 cut(s) 124, 349, 364, 450
TaiI ACGT 2 cut(s) 22, 698
TaqI TCGA 3 cut(s) 81, 321, 452
TasI AATT 4 cut(s) 116, 410, 535, 565
TfiI GAWTC 4 cut(s) 221, 278, 356, 728
Tru1I TTAA 1 cut(s) 665
Tru9I TTAA 1 cut(s) 665
TscAI CASTG 2 cut(s) 321, 487
TseFI GTSAC 1 cut(s) 422
TseI GCWGC 2 cut(s) 440, 673
Tsp45I GTSAC 1 cut(s) 422
TspDTI ATGAA 4 cut(s) 104, 395, 578, 624
TspGWI ACGGA 1 cut(s) 506
TspRI CASTG 2 cut(s) 321, 487
Van91I CCANNNNNTGG 1 cut(s) 722
XapI RAATTY 3 cut(s) 410, 535, 565
XcmI CCANNNNNNNNNTGG 1 cut(s) 740
XmiI GTMKAC 1 cut(s) 33
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.